Biological organic bacterial fertilizer capable of improving plant disease resistance

Abstract

The invention relates to the technical field of agriculture, and discloses a biological organic bacterial fertilizer capable of improving plant disease resistance, which comprises the following components in percentage by mass: 30-50% of bacillus liquid; 30%-50% of a trichoderma bacteria solution; 5%-10% of a chitosan solution; 5%-15% of a sodium alginate solution; 2%-5% of a composite solution; 1% to 5% of a nanocomposite; the composite solution comprises the following components in percentage by mass: 15%-18% of artemisia capillaris; 7%-12% of tripterygium wilfordii; 9%-17% of radix astragali; 15%-19% of fructus cnidii; and 12%-17% of raw licorice. Through effective combination of traditional Chinese medicine components and beneficial bacteria, the insect pest resistance and disease resistance of crops are remarkably enhanced, breeding of aphids, red spiders and other pests can be effectively inhibited, fungal diseases such as downy mildew, powdery mildew and the like are prevented, the immunoreaction of plants is enhanced, and the disease incidence rate is fundamentally reduced.

Description

Technical Field

[0001] The invention relates to the technical field of agriculture, in particular to a biological organic fertilizer capable of improving plant disease resistance. Background Art

[0002] In modern agricultural production, crop pest management is a long-term challenge. Over-reliance on chemical pesticides not only easily leads to pest resistance, but also may cause problems such as pesticide residues and environmental pollution, affecting the balance of the ecosystem and human health. As the concepts of green agriculture and sustainable development are deeply rooted in people's hearts, reducing the use of chemical pesticides and adopting more eco-friendly and pollution-free biological control methods have become an important direction for the development of modern agriculture.

[0003] Existing biofertilizers are not effective in improving crop resistance to pests and diseases. Although some biofertilizers can improve soil health through the action of microorganisms, their inhibitory effects on specific pests and pathogens are limited. Especially in the case of severe pests and diseases, crops still rely on a large amount of chemical pesticides to control pests and diseases, which not only increases the use of chemical pesticides, but also leads to serious pesticide residue problems, and crop quality and safety cannot be guaranteed.

[0004] To this end, those skilled in the art provide a biological organic fertilizer that can improve plant disease resistance to solve the problems raised in the above background technology. Summary of the invention

[0005] In view of the deficiencies of the prior art, the present invention provides a biological organic fertilizer with the function of improving plant disease resistance, thereby solving the problem of poor pest and disease resistance in the prior art.

[0006] To achieve the above object, the present invention is implemented by the following technical scheme: a biological organic fertilizer with improved plant disease resistance, comprising the following components in percentage by mass:

[0007] 30%-50% Bacillus liquid;

[0008] 30%-50% Trichoderma liquid;

[0009] 5%-10% chitosan solution;

[0010] 5%-15% sodium alginate solution;

[0011] 2%-5% compound solution;

[0012] 1%-5% nanocomposite material.

[0013] Preferably, the composite solution comprises the following components in percentage by mass:

[0014] 15%-18% Artemisia capillaris;

[0015] 7%-12% Tripterygium wilfordii;

[0016] 9%-17% astragalus;

[0017] 15%-19% Cnidium monnieri;

[0018] 12%-17% raw licorice;

[0019] 15%-20% of Radix Trichosanthis.

[0020] Preferably, the composite solution is prepared by the following extraction process:

[0021] Mix Artemisia capillaris, Tripterygium wilfordii, Astragalus membranaceus, Cnidium monnieri, Licorice root and Stemona tuber according to the mass ratio, and add water to 8-10 times the weight of the medicinal materials;

[0022] The above mixture is subjected to hot reflux extraction at 60°C-80°C for 2H-4H;

[0023] The extract is filtered, and the filtrate is concentrated under reduced pressure to 1 / 5-1 / 10 of the original volume;

[0024] Place the concentrated solution in a cooling device, cool it to 5°C-10°C, and let it stand for 12H-24H to promote precipitation;

[0025] The cooled precipitate is filtered, and the filtrate is taken as the Chinese medicinal material extraction part of the composite solution;

[0026] The obtained extract is evenly mixed with potassium humate to prepare a composite solution.

[0027] Preferably, the concentration range of the Bacillus and Trichoderma liquid is 1×10 7 -1×10 9 CFU / mL.

[0028] Preferably, the chitosan solution has a concentration range of 2%-3% (w / v), is dissolved in an acetic acid aqueous solution, and the pH value is adjusted to 5.0-6.0.

[0029] Preferably, the concentration of the sodium alginate solution is in the range of 2%-5% (w / v), the nanocomposite material comprises nano-titanium dioxide and nano-silicon, the particle size of which is in the range of 20nm-50nm, and Tween 80 is used as a dispersant.

[0030] A method for preparing a biological organic fertilizer capable of improving plant disease resistance, which is used for a biological organic fertilizer capable of improving plant disease resistance, comprises the following steps:

[0031] S1. Cultivate Bacillus and Trichoderma to appropriate concentrations respectively, and mix the two at a mass percentage of 30%-50% to obtain a mixed bacterial solution;

[0032] S2, preparing coating materials, including chitosan solution, sodium alginate solution, Artemisia capillaris, Tripterygium wilfordii, Astragalus, Stemona tuber, Cnidium monnieri, raw licorice and nanocomposite dispersion;

[0033] S3, mixing the mixed bacterial solution with the chitosan solution in a ratio of 1:1, and performing microencapsulation treatment using a spray drying technique to obtain coated particles;

[0034] S4, coating the coated particles with the nanocomposite material by electrostatic spraying;

[0035] S5. Form a multi-layer coating structure through a layer-by-layer deposition process.

[0036] Preferably, the feed temperature during spray drying in step S3 is 50°C-60°C, the discharge temperature is 40°C-60°C, and the gas flow rate is set to 1.0m 3 / min-1.5m 3 / min.

[0037] Preferably, when the nanomaterial is coated by electrostatic spraying in step S4, the spraying voltage is 12kV-15kV, and after coating, it is dried at 30°C-40°C, each drying time is 15min-20min, and repeated 3-5 times.

[0038] Preferably, the multi-layer coating structure is formed by sequentially depositing chitosan, sodium alginate, Artemisia capillaris, Tripterygium wilfordii, Astragalus, Stemona tuber, Cnidium monnieri, raw liquorice and nanocomposite materials to ultimately form a 3-5-layer coating structure.

[0039] The present invention provides a biological organic fertilizer capable of improving plant disease resistance. It has the following beneficial effects:

[0040] The invention significantly enhances the pest and disease resistance of crops through the effective combination of traditional Chinese medicine components and beneficial bacteria. Traditional Chinese medicine components such as stemonae, tripterygium wilfordii and cnidium monnieri have good insecticidal and antibacterial effects, can effectively inhibit the reproduction of pests such as aphids and red spiders, and prevent fungal diseases such as downy mildew and powdery mildew, enhance the immune response of plants, and fundamentally reduce the incidence of diseases. Experiments show that after using the bacterial fertilizer, the disease inhibition rate of crops reaches 85%-90%, and the pest reduction rate reaches 80%-85%, which significantly improves the health level and stress resistance of crops. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 It is a schematic diagram of the preparation process of the present invention. DETAILED DESCRIPTION

[0042] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0043] Please refer to the attached Figure 1 Example 1: Preparation of biological organic fertilizer with astragalus as the main ingredient

[0044] Recipe composition:

[0045] Bacillus liquid: 40%

[0046] Trichoderma liquid: 35%

[0047] Chitosan solution: 7%

[0048] Sodium alginate solution: 10%

[0049] Compound solution: 5%

[0050] Nanocomposite materials: 3%

[0051] Composite solution ingredients:

[0052] Artemisia capillaris: 16%

[0053] Tripterygium wilfordii: 10%

[0054] Astragalus: 15%

[0055] Cnidium monnieri: 18%

[0056] Raw licorice: 14%

[0057] 100: 17%

[0058] Preparation steps:

[0059] Bacterial culture:

[0060] Bacillus and Trichoderma were cultured to appropriate concentrations, respectively, controlled at 1×10 8 CFU / mL, and mixed at 40% and 35% by mass to obtain a mixed bacterial solution.

[0061] Extraction of Chinese herbal medicine:

[0062] Artemisia capillaris, Tripterygium wilfordii, Astragalus membranaceus, Cnidium monnieri, Licorice root and Stemona tuberosa were mixed in proportion, water was added to 10 times the weight of the medicinal materials, and hot reflux extraction was performed at 70°C for 3 hours. After the extract was filtered, it was concentrated to 1 / 7 of the original volume under reduced pressure, cooled to 5°C, and allowed to stand for 16 hours. The filtrate was filtered and mixed with potassium humate to obtain a composite solution.

[0063] Preparation of Nanocomposites:

[0064] Nano titanium dioxide and nano silicon are mixed in proportion, the particle size is controlled at 20nm-50nm, and Tween 80 is used as a dispersant to disperse into a solution for later use.

[0065] Mixing and coating:

[0066] The mixed bacterial solution and chitosan solution were mixed in a ratio of 1:1 and microencapsulated using spray drying technology. The feed temperature was 55°C, the discharge temperature was 45°C, and the gas flow rate was 1.2m 3 / min to obtain coated particles.

[0067] Electrostatic spraying of nanomaterials:

[0068] The coated particles were coated with the nanocomposite material by electrostatic spraying, the spraying voltage was set to 12 kV, and after coating, they were dried at 30° C. for 20 minutes, which was repeated 3 times.

[0069] Layer-by-layer deposition forms a multi-layer coating:

[0070] Chitosan, sodium alginate, composite solution (including Chinese medicinal ingredients such as astragalus) and nanocomposite materials are deposited layer by layer through the layer-by-layer process to eventually form a three-layer coating structure.

[0071] Summary: Astragalus, as the main Chinese medicinal ingredient, combined with Bacillus and Trichoderma, not only significantly enhances the immunity of plants, but also promotes the growth and yield of crops through its growth-promoting effect. The polysaccharides and flavonoids in Astragalus enhance the activity of plant roots, promote the root system's ability to absorb water and nutrients, and thus accelerate the growth of crops.

[0072] Growth-promoting effect: Plants grow faster, their roots are stronger, and yields are significantly increased (in the experiment, the growth-promoting effect of Astragalus increased yields by about 15%). It improves the plant microbial environment and further promotes plant nutrient absorption.

[0073] Example 2: Preparation of biological organic fertilizer with Tripterygium wilfordii as the main ingredient

[0074] Recipe composition:

[0075] Bacillus liquid: 35%

[0076] Trichoderma liquid: 40%

[0077] Chitosan solution: 7%

[0078] Sodium alginate solution: 10%

[0079] Compound solution: 5%

[0080] Nanocomposite materials: 3%

[0081] Composite solution ingredients:

[0082] Artemisia capillaris: 15%

[0083] Tripterygium wilfordii: 9%

[0084] Astragalus: 17%

[0085] Cnidium monnieri: 19%

[0086] Raw licorice: 12%

[0087] Centipede: 18%

[0088] Preparation steps:

[0089] Bacterial culture:

[0090] Bacillus and Trichoderma were cultured separately to a concentration of 1×10 8 CFU / mL, and mixed in a ratio of 35% and 40% to obtain a mixed bacterial solution.

[0091] Extraction of Chinese herbal medicine:

[0092] Artemisia capillaris, Tripterygium wilfordii, Astragalus membranaceus, Cnidium monnieri, Licorice root and Stemona tuberosa were mixed in proportion, water was added to 8 times the weight of the medicinal materials, and hot reflux extraction was performed at 60°C for 3 hours. The extract was filtered, concentrated under reduced pressure to 1 / 7 of the original volume, cooled to 5°C, allowed to stand for 12 hours, filtered, and mixed with potassium humate to obtain a composite solution.

[0093] Preparation of Nanocomposites:

[0094] Nano titanium dioxide and nano silicon oxide are used, the particle size is controlled at 30nm, and Tween 80 dispersant is used.

[0095] Mixing and coating:

[0096] The mixed bacterial solution was mixed with chitosan solution in a ratio of 1:1 and spray dried at a feed temperature of 60°C, a discharge temperature of 50°C, and a gas flow rate of 1.5 m 3 / min to obtain coated particles.

[0097] Electrostatic spraying treatment:

[0098] The coated particles were electrostatically sprayed with nanocomposites, the spraying voltage was set to 13 kV, and after spraying, they were dried at 35° C. for 15 minutes, which was repeated 4 times.

[0099] Layer-by-layer deposition forms a multi-layer coating:

[0100] Chitosan, sodium alginate and composite solution (such as Tripterygium wilfordii) are deposited in sequence using a layer-by-layer process to form a four-layer coating structure.

[0101] Summary: The combination of the powerful antibacterial and anti-inflammatory properties of Tripterygium wilfordii with Bacillus and Trichoderma can form a dual inhibitory effect on fungi and bacteria. The active ingredients in Tripterygium wilfordii have broad-spectrum antibacterial properties, which can directly destroy the cell walls of pathogens and inhibit the growth of fungi and bacteria. Combined with Bacillus and Trichoderma in the beneficial bacterial liquid, this bacterial fertilizer can effectively reduce the number of pathogens in the soil and establish a dominant community of beneficial microorganisms. This formula is particularly effective in combating diseases caused by mold (such as powdery mildew, downy mildew, etc.) and can significantly reduce the frequency of disease occurrence.

[0102] Example 3: Preparation of biological organic fertilizer with Stemona tuberosa as main component

[0103] Recipe composition:

[0104] Bacillus liquid: 40%

[0105] Trichoderma liquid: 35%

[0106] Chitosan solution: 6%

[0107] Sodium alginate solution: 10%

[0108] Compound solution: 5%

[0109] Nanocomposite materials: 4%

[0110] Composite solution ingredients:

[0111] Artemisia capillaris: 18%

[0112] Tripterygium wilfordii: 12%

[0113] Astragalus: 16%

[0114] Cnidium monnieri: 15%

[0115] Raw licorice: 17%

[0116] 100: 17%

[0117] Preparation steps:

[0118] Bacterial culture:

[0119] Bacillus and Trichoderma are cultured separately to appropriate concentrations and mixed according to proportion to obtain a mixed bacterial solution.

[0120] Extraction of Chinese herbal medicine:

[0121] Artemisia capillaris, Tripterygium wilfordii, Astragalus membranaceus, Cnidium monnieri, Licorice root and Stemona tuberosa were mixed in proportion, water was added to 10 times the weight of the medicinal materials, and hot reflux extraction was performed at 70°C for 3 hours. After the extract was filtered, it was concentrated to 1 / 8 volume under reduced pressure, cooled to 7°C, allowed to stand for 18 hours, and filtered to obtain a composite solution.

[0122] Preparation of Nanocomposites:

[0123] A mixture of nano-silicon dioxide and nano-zinc oxide was used as the nanocomposite material, the particle size was controlled at 20 nm, and Tween 80 was used for dispersion.

[0124] Mixing and coating:

[0125] The mixed bacterial solution was mixed with the chitosan solution, and subjected to spray drying treatment, with a feed temperature of 50° C. and a discharge temperature of 45° C., to obtain coated particles.

[0126] Electrostatic spraying treatment:

[0127] The nanocomposite material was electrostatically sprayed at a spraying voltage of 15 kV, a drying temperature set at 40°C, and each drying time was 20 minutes, which was repeated 3 times.

[0128] Layer-by-layer deposition forms a multi-layer coating:

[0129] Chitosan, sodium alginate and composite solution are deposited in sequence to eventually form a four-layer coating structure.

[0130] Summary: As the main Chinese medicine ingredient, Stemona officinalis, in combination with beneficial bacteria liquid, exhibits powerful insecticidal and antibacterial effects. The alkaloid components contained in Stemona officinalis not only have a strong killing effect on a variety of plant pests (such as aphids, red spiders, etc.), but also can inhibit the spread of pathogens by combining with Trichoderma. Beneficial bacteria improve the soil microenvironment and promote the reproduction of beneficial bacteria, thereby further enhancing the control effect on pests. This embodiment is particularly suitable for preventing and controlling pests and inhibiting secondary diseases caused by pests, reducing the use of pesticides, and improving plant resistance to insects.

[0131] Example 4: Preparation of biological organic fertilizer with Artemisia capillaris as the main ingredient

[0132] Recipe composition:

[0133] Bacillus liquid: 45%

[0134] Trichoderma liquid: 30%

[0135] Chitosan solution: 7%

[0136] Sodium alginate solution: 10%

[0137] Compound solution: 5%

[0138] Nanocomposite materials: 3%

[0139] Composite solution ingredients:

[0140] Artemisia capillaris: 17%

[0141] Tripterygium wilfordii: 8%

[0142] Astragalus: 14%

[0143] Cnidium monnieri: 19%

[0144] Raw licorice: 15%

[0145] 100: 17%

[0146] Preparation steps:

[0147] Bacillus and Trichoderma are cultured separately to appropriate concentrations and mixed according to proportion to obtain a mixed bacterial solution.

[0148] Extraction of Chinese herbal medicine:

[0149] Artemisia capillaris, Tripterygium wilfordii, Astragalus membranaceus, Cnidium monnieri, Licorice root and Stemona tuberosa were mixed in proportion, water was added to 9 times the weight of the medicinal materials, and hot reflux extraction was performed at a temperature of 60°C for 3 hours. After the extract was filtered, it was concentrated under reduced pressure to 1 / 6 of the original volume, cooled to 5°C, allowed to stand for 16 hours, and filtered to obtain a composite solution.

[0150] Preparation of Nanocomposites:

[0151] A mixture of nano zinc oxide and nano titanium dioxide is used as a nano composite material, the particle size is controlled at 20-40 nm, and Tween 80 is used as a dispersant.

[0152] Mixing and coating:

[0153] The mixed bacterial solution and chitosan solution were mixed in a ratio of 1:1 and spray dried at a feed temperature of 55°C, a discharge temperature of 50°C, and a gas flow rate of 1.0 m 3 / min to obtain coated particles.

[0154] Electrostatic spraying treatment:

[0155] The coated particles were electrostatically sprayed with nanocomposites, the spraying voltage was set to 13 kV, the drying temperature after spraying was set to 35°C, each drying time was 20 minutes, and repeated 3 times.

[0156] Layer-by-layer deposition forms a multi-layer coating:

[0157] Chitosan, sodium alginate and the composite solution are deposited in sequence to form a 3-4 layer coating structure.

[0158] Summary: The combination of Artemisia capillaris and beneficial bacteria (Bacillus, Trichoderma) is effective in improving plant stress resistance. Artemisia capillaris has antioxidant and anti-inflammatory properties, which can enhance the adaptability of plants to harsh environments (such as drought and saline-alkali land). The use of Bacillus and Trichoderma can reduce the physiological stress of plants in harsh environments.

[0159] Example 5: Preparation of biological organic fertilizer with Cnidium monnieri as main ingredient

[0160] Recipe composition:

[0161] Bacillus liquid: 40%

[0162] Trichoderma liquid: 40%

[0163] Chitosan solution: 6%

[0164] Sodium alginate solution: 9%

[0165] Compound solution: 4%

[0166] Nanocomposite materials: 1%

[0167] Composite solution ingredients:

[0168] Artemisia capillaris: 15%

[0169] Tripterygium wilfordii: 10%

[0170] Astragalus: 16%

[0171] Cnidium monnieri: 18%

[0172] Raw licorice: 15%

[0173] 100: 16%

[0174] Preparation steps:

[0175] Bacterial culture:

[0176] Bacillus and Trichoderma were cultured to a concentration of 1×108 CFU / mL respectively, and mixed according to proportion to obtain a 40% and 40% mixed bacterial solution.

[0177] Extraction of Chinese herbal medicine:

[0178] Artemisia capillaris, Tripterygium wilfordii, Astragalus membranaceus, Cnidium monnieri, Licorice root and Stemona tuberosa were mixed in proportion, water was added to 10 times the weight of the medicinal materials, and hot reflux extraction was performed at 65°C for 3 hours. After the extract was filtered, it was concentrated under reduced pressure to 1 / 7 of the original volume, cooled to 5°C, allowed to stand for 12 hours, and filtered to obtain a composite solution.

[0179] Preparation of Nanocomposites:

[0180] A mixture of nano-titanium dioxide and nano-silicon dioxide is used, the particle size is controlled at 25-35nm, and Tween 80 is used as a dispersant.

[0181] Mixing and coating:

[0182] The mixed bacterial solution and chitosan solution were mixed in a ratio of 1:1, and spray-dried at a feed temperature of 60°C and a discharge temperature of 50°C to obtain coated particles.

[0183] Electrostatic spraying treatment:

[0184] The coated particles were electrostatically sprayed with nanocomposites, the spraying voltage was set to 12 kV, and dried at 30° C. after spraying, each drying time was 15 minutes, and repeated 4 times.

[0185] Layer-by-layer deposition forms a multi-layer coating:

[0186] Chitosan, sodium alginate, composite solution and nanocomposite materials are deposited in sequence through a layer-by-layer process to form a 3-5-layer coating structure.

[0187] Summary: As the main ingredient of traditional Chinese medicine, Cnidium monnieri combined with beneficial bacteria (especially Trichoderma) exhibits a strong antifungal effect. The active ingredients in Cnidium monnieri have significant antibacterial and antifungal properties, which can effectively prevent and treat fungal diseases on plant roots and leaves. The role of Trichoderma in inhibiting the growth of pathogens in the soil further enhances the performance of Cnidium monnieri in bacterial fertilizer. This formula is particularly effective in preventing and treating fungal diseases such as plant root rot, powdery mildew, and rust. By promoting the growth of beneficial bacteria, it significantly reduces the occurrence of diseases and enhances the health of plant roots.

[0188] Experimental Example 1: Experiment on the inhibitory effect of biological organic fertilizer on plant fungal diseases

[0189] Purpose:

[0190] The inhibitory effects of the biological organic fertilizers of five different embodiments on plant fungal diseases (such as downy mildew and powdery mildew) were compared.

[0191] Experimental Materials:

[0192] Test plants: cucumber, tomato (plants susceptible to fungal diseases)

[0193] Diseases: downy mildew, powdery mildew

[0194] Experimental fertilizer: biological organic fertilizer of Examples 1-5

[0195] Control group: treatment group without using bacterial fertilizer, treatment group using only chemical fungicide

[0196] Experimental methods:

[0197] Pretreatment: Cucumber and tomato seeds were cultivated under the same conditions and grown to the same height. Then, they were inoculated with downy mildew and powdery mildew pathogens in a greenhouse, respectively.

[0198] Experimental group treatment:

[0199] The biological organic fertilizers of Examples 1-5 were applied to the diseased plants respectively, sprayed once every 7 days, for 4 consecutive times.

[0200] Control group 1: No bacterial fertilizer was used, only basic irrigation was performed.

[0201] Control group 2: Standard chemical fungicide was used (compared with traditional treatment).

[0202] Observation indicators:

[0203] Percentage of lesion area: record the percentage of lesion area on plant leaves after the disease occurs.

[0204] Disease Development Rate: The rate of disease spread was assessed 2 and 4 weeks after inoculation.

[0205] Plant health score: Rated from 1 (severe infection) to 5 (no infection).

[0206] Experimental results (data table):

[0207]

[0208] Experimental analysis:

[0209] Example 2 (Tripterygium wilfordii) and Example 5 (Fructus Cnidium monnieri) have the best effect in inhibiting fungal diseases (downy mildew and powdery mildew), showing excellent antifungal properties.

[0210] Example 1 (Astragalus) also effectively slowed down the spread of diseases and enhanced plant immunity.

[0211] The disease in the control group 1 (untreated group) was severe and spread rapidly, indicating that the plant was highly susceptible to fungi.

[0212] Although the control group 2 (chemical fungicide group) has the best disease suppression effect, long-term use may lead to drug resistance problems and pollute the environment.

[0213] Experimental Example 2: Experiment on the control effect of biological organic fertilizer on plant pests

[0214] Purpose:

[0215] The control effects of the biological organic fertilizers in the five examples on common plant pests (such as aphids and red spiders) were evaluated.

[0216] Experimental Materials:

[0217] Test plants: tomatoes, peppers

[0218] Pest types: aphids, spider mites

[0219] Experimental fertilizer: biological organic fertilizer of Examples 1-5

[0220] Control group: untreated plants, conventional chemical pesticides

[0221] Experimental methods:

[0222] Pretreatment: Grow tomatoes and peppers in a greenhouse, inoculate with aphids and spider mites, and start treatment after the pests have obviously multiplied.

[0223] Experimental group treatment:

[0224] The biological organic fertilizers of Examples 1-5 were sprayed on the plants respectively, and the spraying was continued for 4 times, with an interval of 7 days between each spraying.

[0225] Control group 1: No control measures were applied.

[0226] Control group 2: sprayed with conventional chemical insecticides.

[0227] Observation indicators:

[0228] Pest population: The number of pests on each leaf.

[0229] Plant damage degree: By visual inspection, the degree of leaf damage was assessed (1-5 points).

[0230] Plant recovery: Assess the health of plants after pest control.

[0231] Experimental results (data table):

[0232]

[0233]

[0234] Experimental analysis:

[0235] Example 3 (Stemella) performed best in controlling pests, especially against aphids and red spiders, with a pest reduction rate of 85%.

[0236] Example 5 (Fructus Cnidium monnieri) and Example 2 (Tripterygium wilfordii) also showed good insect repellent effects, which could significantly reduce the number of pests and restore the health of the plants.

[0237] Control group 2 (chemical pesticide group) showed the highest pest control ability, but the prolonged use of chemicals may have adverse effects on the environment and is not good for the sustainable health of plants.

[0238] Comparative experimental design:

[0239] In order to further verify the effect of the embodiment and compare it with the conventional treatment method, the following comparative experiment was designed:

[0240] Comparative experiment 1: Comparison of disease resistance between biological organic fertilizer and chemical fertilizer

[0241] Purpose:

[0242] Compare the effects of biological organic fertilizer and chemical fertilizer on plant disease resistance and evaluate the difference in their effects.

[0243] Experimental Materials:

[0244] Test plants: tomatoes, peppers (susceptible to diseases)

[0245] Experimental bacterial fertilizer: Example 1 and Example 5

[0246] Control group: conventional chemical fertilizer (nitrogen, phosphorus and potassium compound fertilizer)

[0247] Experimental methods:

[0248] Under the same planting environment, apply the same amount of chemical fertilizer and bacterial fertilizer.

[0249] Common diseases of inoculated plants (e.g., gray mold, wilt).

[0250] Regularly test plants for disease development and health scores after fertilization.

[0251] Observation indicators:

[0252] Disease suppression rate, plant health score, plant yield;

[0253] Experimental results:

[0254] Experimental Group Disease inhibition rate (%) Health score (1-5) Change in output (%) Example 1 85 4.3 +15 Example 5 88 4.5 +14 Chemical fertilizers 65 3.5 +8

[0255] Experimental analysis:

[0256] The bacterial fertilizers of Example 1 and Example 5 both showed excellent disease inhibition ability. Compared with chemical fertilizers, the disease inhibition rate was more than 20% higher, and the health score and yield were also significantly better than those of the chemical fertilizer group.

[0257] Although chemical fertilizers have certain effects, their long-term use may lead to soil acidification and microbial imbalance, affecting the long-term health of crops.

[0258] Comparative experiment 2: Comparison of biological organic fertilizer and chemical fungicide in resistance to fungal diseases

[0259] Purpose:

[0260] To evaluate the difference in effectiveness between bio-organic fertilizer and conventional chemical fungicides in resisting fungal diseases.

[0261] Experimental Materials:

[0262] Test plants: wheat, soybean

[0263] Experimental bacterial fertilizer: Example 2 and Example 4

[0264] Control group: standard chemical fungicide

[0265] Experimental methods:

[0266] After inoculation of fungal diseases (such as powdery mildew and rust), use bacterial fertilizers and fungicides to treat them respectively.

[0267] Monitor disease development regularly.

[0268] Observation indicators:

[0269] Fungal disease inhibition rate, plant growth, and plant yield;

[0270] Experimental results:

[0271]

[0272] Experimental analysis:

[0273] The inhibitory effects of Example 2 (Tripterygium wilfordii) and Example 4 (Artemisia capillaris) on fungal diseases are close to those of chemical fungicides.

[0274] Although fungicides have slightly higher disease suppression rates, they may have long-term negative impacts on the environment and plant health.

[0275] Comparative experiment 3: Comparison between biological organic fertilizer and chemical pesticides in pest control

[0276] Purpose:

[0277] Compare the effects of biological organic fertilizer and chemical pesticides in controlling plant pests (such as aphids and mites).

[0278] Experimental Materials:

[0279] Test plants: cucumber, eggplant

[0280] Pests: aphids, spider mites

[0281] Experimental bacterial fertilizer: Example 3 and Example 5

[0282] Control group: conventional chemical pesticides

[0283] Experimental methods:

[0284] Inoculate aphids and red spiders, and apply bacterial fertilizers and pesticides for prevention and control.

[0285] Monitor pest populations and plant health weekly.

[0286] Observation indicators:

[0287] Pest reduction rate, plant damage score, plant yield;

[0288] Experimental results:

[0289] Experimental Group Pest reduction rate (%) Victimization score (1-5) Change in output (%) Example 3 85 4.2 +13 Example 5 80 4.0 +12 Insecticides 95 4.6 +10

[0290] Experimental analysis:

[0291] Example 3 (Stemella) showed a very strong insecticidal effect, close to the chemical insecticide group, but it had less negative impact on the environment and plant health, and improved the overall health of the plant through the action of beneficial bacteria.

[0292] Example 5 (Cnidium monnieri) also has a good inhibitory effect on pests, and the plant yield is significantly increased.

[0293] Although chemical pesticides have high pest reduction rates, they may have adverse effects on the environment and soil microorganisms.

[0294] Summarize:

[0295] These three sets of comparative experiments fully demonstrate the excellent performance of bio-organic fertilizer in disease resistance, insect prevention, improving plant health and adapting to the environment. The effect of bio-organic fertilizer is close to or even better than chemical fertilizers and chemical pesticides. At the same time, it is more environmentally friendly, can maintain soil health and biodiversity, and is suitable for long-term sustainable agricultural production.

[0296] Research system:

[0297] This includes research on plant performance in different areas: yield, disease resistance, pest control, fungal inhibition, cold resistance, and high temperature tolerance. The following is how to incorporate these research directions into the research system, combined with the specific examples of the above-mentioned Chinese herbal fungi.

[0298] Research system framework

[0299] 1. Yield improvement research

[0300] Research objective: To evaluate the effect of Chinese herbal fungi on increasing crop yields, especially the effect of combining Chinese herbal ingredients (such as Astragalus and Stemona) with beneficial bacteria on crop growth rate and final yield.

[0301] Research direction:

[0302] Experimental group: Examples of bacterial fertilizers using different Chinese medicinal ingredients (such as Astragalus, Artemisia capillaris, and Cnidium monnieri) (Examples 1-5).

[0303] Metrics: Crop growth rate, root development, plant height, leaf area, and total harvested yield.

[0304] Data Analysis:

[0305] The bacterial fertilizer group containing growth-promoting Chinese herbal ingredients (such as astragalus) was compared with the control group without using Chinese herbal medicine to analyze the increase in yield.

[0306] Expected results: The examples containing Astragalus (such as Example 1) are expected to significantly increase crop yields by promoting root development and enhancing photosynthesis (yield increased by 15%-20% in the experiment).

[0307] 2. Disease resistance research

[0308] Research objective: To verify the effectiveness of traditional Chinese medicine in combating plant diseases, especially the inhibitory effects on fungal and bacterial diseases.

[0309] Research direction:

[0310] Experimental group: The bacterial fertilizers in Examples 1-5 were tested for their resistance to different diseases (such as wilt, downy mildew, rust, etc.).

[0311] Indicators: disease inhibition rate, lesion area, disease spread rate, plant health score.

[0312] Data Analysis:

[0313] The inhibitory effects of each bacterial fertilizer group on different diseases were compared, and the effects of bacterial fertilizers containing antibacterial ingredients such as Tripterygium wilfordii and Cnidium monnieri (such as Examples 2 and 5) were studied in detail.

[0314] Expected results: The bacterial fertilizer containing Tripterygium wilfordii (Example 2) has a significant inhibitory effect on fungal diseases, and the disease inhibition rate can reach more than 90%.

[0315] 3. Pest control research

[0316] Research objective: To evaluate the efficacy of Chinese herbal fungi on pest control, especially the inhibition of common pests such as aphids and mites.

[0317] Research direction:

[0318] Experimental group: Compare the control effects of Example 3 (using Stemona as the main ingredient) and other examples on plant pests.

[0319] Indicators: Pest reduction rate, plant damage area, and plant recovery status.

[0320] Data Analysis:

[0321] The inhibitory effect of Stemona officinalis combined with beneficial bacteria on pests was analyzed and compared with the effect of conventional chemical pesticides.

[0322] Expected results: As an insecticide, the combination of Stemona japonica and beneficial bacteria can significantly reduce the number of pests, and the pest reduction rate is expected to be between 80% and 85%.

[0323] 4. Fungal inhibition study

[0324] Research objective: To test the inhibitory effect of Chinese herbal agents on fungal diseases, especially the prevention and control of fungal diseases such as root rot and mildew.

[0325] Research direction:

[0326] Experimental group: Example 2 (Tripterygium wilfordii) and Example 5 (Cnidium monnieri) were used to test common fungal diseases.

[0327] Indicators: inhibition rate of fungal diseases, number of fungal spores, and health of plant roots.

[0328] Data Analysis:

[0329] The differences in fungal inhibition effects among the examples of the bacterial fertilizers were analyzed, the inhibition mechanisms of Tripterygium wilfordii and Fructus cnidium were studied, and their synergistic effects with Trichoderma were evaluated.

[0330] Expected results: Tripterygium wilfordii and Cnidium monnieri can effectively inhibit fungal diseases such as mold, with a disease inhibition rate of 85%-90%.

[0331] Research direction:

[0332] Experimental group: Example 1 (Astragalus) and Example 4 (Artemisia capillaris) were tested for their effects on crop growth under high temperature conditions.

[0333] Indicators: plant survival rate, leaf wilting rate, and plant photosynthesis efficiency under high temperature conditions.

[0334] Data Analysis:

[0335] The health status of plants in each bacterial fertilizer group under high temperature conditions was analyzed, focusing on the role of Astragalus and Artemisia capillaris in high temperature tolerance.

[0336] Expected results: Astragalus and Artemisia capillaris significantly enhance the tolerance of crops to high temperatures, maintain the stability of plant growth, and increase the survival rate by 10%-15%.

[0337] Comprehensive data analysis Based on the experimental data of the above research directions and combined with the performance of Chinese herbal fungi on plants in different environments, the research system will cover the following important aspects:

[0338] Yield increase: Chinese herbal ingredients such as astragalus, when combined with beneficial bacteria, can significantly increase crop yields by 10%-20% by enhancing plant root activity and photosynthesis efficiency.

[0339] Disease resistance: The addition of Chinese medicinal ingredients such as Tripterygium wilfordii and Cnidium monnieri enhances the disease resistance of the fertilizer, especially its inhibitory effect on fungal and bacterial diseases. The disease inhibition rate can be as high as 85%-90%.

[0340] Pest control: Fertilizers containing Stemona japonica ingredients show good insecticidal effects, with a pest reduction rate of 80%-85%, and can effectively prevent and control pests such as aphids and mites.

[0341] Fungal inhibition: The combination of Chinese herbs such as Tripterygium wilfordii and Cnidium monnieri with Trichoderma can significantly inhibit fungal diseases and help plants resist fungal diseases such as root rot and powdery mildew.

[0342] Cold resistance and stress resistance: Ingredients such as Artemisia capillaris and Astragalus membranaceus improve the plant's ability to adapt to low and high temperature environments, enhance the plant's stress resistance, and enable it to continue to grow normally in harsh environments.

[0343] High temperature tolerance: Astragalus and Artemisia capillaris perform well in high temperature environments and help improve the growth stability of crops under hot conditions.

[0344] Through the above-mentioned research directions and experiments, the advantages of Chinese herbal fungi in promoting crop growth, improving disease and insect resistance, and adapting to the environment can be fully demonstrated.

[0345] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A biological organic fertilizer capable of improving plant disease resistance, characterized in that: The following components are included in mass percentage: 30%-50% Bacillus liquid; 30%-50% Trichoderma liquid; 5%-10% chitosan solution; 5%-15% sodium alginate solution; 2%-5% compound solution; 1%-5% nanocomposite material.

2. The biological organic fertilizer for improving plant disease resistance according to claim 1, characterized in that: The composite solution includes the following components in percentage by mass: 15%-18% Artemisia capillaris; 7%-12% Tripterygium wilfordii; 9%-17% astragalus; 15%-19% Cnidium monnieri; 12%-17% raw liquorice; 15%-20% of Radix Stemonae.

3. The biological organic fertilizer for improving plant disease resistance according to claim 1, characterized in that: The composite solution is prepared by the following extraction process: Mix Artemisia capillaris, Tripterygium wilfordii, Astragalus membranaceus, Cnidium monnieri, Licorice root and Stemona tuber according to the mass ratio, and add water to 8-10 times the weight of the medicinal materials; The above mixture is subjected to hot reflux extraction at 60°C-80°C for 2H-4H; The extract was filtered and the filtrate was concentrated under reduced pressure to 1 / 5-1 / 10 of the original volume; Place the concentrated solution in a cooling device, cool it to 5°C-10°C, and let it stand for 12H-24H to promote precipitation; The cooled precipitate is filtered, and the filtrate is taken as the Chinese medicinal material extraction part of the composite solution; The obtained extract is evenly mixed with potassium humate to prepare a composite solution.

4. The biological organic fertilizer for improving plant disease resistance according to claim 1, characterized in that: The concentration range of the Bacillus and Trichoderma liquid is 1×10 7 -1×10 9 CFU / mL.

5. The biological organic fertilizer for improving plant disease resistance according to claim 1, characterized in that: The chitosan solution has a concentration range of 2%-3% (w / v), is dissolved in an acetic acid aqueous solution, and the pH value is adjusted to 5.0-6.

0.

6. The biological organic fertilizer for improving plant disease resistance according to claim 1, characterized in that: The concentration range of the sodium alginate solution is 2%-5% (w / v), the nano composite material comprises nano titanium dioxide and nano silicon, the particle size range is 20nm-50nm, and Tween 80 is used as a dispersant.

7. A method for preparing a biological organic fertilizer capable of improving plant disease resistance, used for the biological organic fertilizer capable of improving plant disease resistance as claimed in any one of claims 1 to 6, characterized in that: The following steps are involved: S1, culturing Bacillus and Trichoderma to appropriate concentrations respectively, and mixing the two at a mass percentage of 30%-50% to obtain a mixed bacterial solution; S2, preparing coating materials, including chitosan solution, sodium alginate solution, Artemisia capillaris, Tripterygium wilfordii, Astragalus, Stemona tuber, Cnidium monnieri, raw licorice and nanocomposite dispersion; S3, mixing the mixed bacterial solution with the chitosan solution in a ratio of 1:1, and performing microencapsulation treatment using a spray drying technique to obtain coated particles; S4, coating the coated particles with the nanocomposite material by electrostatic spraying; S5. Form a multi-layer coating structure through a layer-by-layer deposition process.

8. The method for preparing a biological organic fertilizer capable of improving plant disease resistance according to claim 7, characterized in that: The feed temperature during spray drying in step S3 is 50°C-60°C, the discharge temperature is 40°C-60°C, and the gas flow rate is set to 1.0m 3 / min-1.5m 3 / min.

9. The method for preparing a biological organic fertilizer capable of improving plant disease resistance according to claim 7, characterized in that: When the nanomaterial is coated by electrostatic spraying in step S4, the spraying voltage is 12kV-15kV. After coating, the nanomaterial is dried at 30°C-40°C for 15min-20min each time, and the process is repeated 3-5 times.

10. The method for preparing a biological organic fertilizer capable of improving plant disease resistance according to claim 7, characterized in that: The multi-layer coating structure is formed by sequentially depositing chitosan, sodium alginate, Artemisia capillaris, Tripterygium wilfordii, Astragalus membranaceus, Stemona tuber, Cnidium monnieri, raw liquorice and nano-composite materials, and finally forms a 3-5-layer coating structure.

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