New food bacteriostasis and fresh-keeping method based on NO atmosphere protection

Abstract

The invention relates to the technical field of nano materials and food safety, in particular to a novel food bacteriostasis and preservation method based on NO atmosphere protection. The preparation method comprises the following steps: carrying out ultrasonic dispersion on a lead-free rare earth-based perovskite material Cs3EuCl6 nanocrystal in water, and then cross-linking with Ce6 to obtain the NO generation photosensitizer Cs3EuCl6-Ce6 based on the cascade reaction. The preparation method comprises the following steps: mixing arginine with a sodium carboxymethyl cellulose (CMC) solution, adding the Cs3EuCl6-Ce6 nanocrystals, stirring, pouring on a glass plate, and drying to obtain the food antibacterial preservative film based on NO atmosphere protection. Under the irradiation of ultraviolet light, the NO generation photosensitizer Cs3EuCl6-Ce6 based on cascade reaction in the food antibacterial preservative film based on NO atmosphere protection rapidly generates active oxygen, and arginine is triggered to release NO through cascade reaction, so that the sterilizing effect is enhanced. The non-contact antibacterial strategy effectively reduces the risks of toxicity and microbial drug resistance, and an efficient, safe and environment-friendly solution is provided for food preservation.

Description

Technical Field

[0001] The present invention relates to the technical field of nanomaterials and food safety, and in particular to a preparation method of a food antibacterial fresh-keeping film based on NO atmosphere protection and application of the film in food preservation. Background Art

[0002] In recent years, with the increasing demand for food safety and freshness preservation, non-contact antibacterial preservation technologies have garnered increasing attention. Food is susceptible to bacterial contamination during storage and distribution, and traditional contact preservation methods (such as antimicrobial films and chemical preservatives) have significant limitations, including cross-contamination, microbial resistance, damage to food flavor and nutrients, and potential harm to consumer health. Consequently, there is an urgent need for efficient, safe, and environmentally friendly preservation technologies. Non-contact antibacterial preservation technologies have emerged as an innovative solution to this problem. Non-contact antibacterial preservation technologies effectively inhibit bacterial growth by forming an antibacterial protective layer on or around the food surface, eliminating the need for direct contact with the food. This reduces damage to food quality and meets consumer demand for natural, additive-free foods. Currently, remote antibacterial technologies such as ultraviolet light irradiation, ozone atmosphere, and modified atmosphere packaging have been applied to food preservation, but these methods still have limitations. For example, ultraviolet light irradiation can cause fat oxidation and loss of nutrients in food; ozone and modified atmosphere packaging can affect food flavor and accumulate harmful substances at high concentrations. Therefore, there is an urgent need for an innovative technology that can provide efficient antibacterial properties without direct contact with food and without changing the flavor and nutrition of food.

[0003] Photodynamically induced NO release from NO donors offers an effective solution. This technology activates photosensitizers through light to produce reactive oxygen species (ROS), which in turn stimulates NO donors to release NO gas around food, thereby inhibiting bacterial growth. NO gas can penetrate bacterial cell walls, disrupting bacterial structure and achieving an antibacterial effect. Unlike traditional contact antibacterial technologies, this process requires no additional chemical additives and has little impact on food flavor and nutritional content, making it environmentally friendly, safe, and highly effective. Among these, chlorin e6 (Ce6), an excellent photosensitizer derived from the careful extraction and chemical modification of chlorophyll, exhibits excellent ROS responsiveness, low dark toxicity, and favorable in vivo metabolic properties. Under red light irradiation, Ce6 can convert photon energy into ROS. However, Ce6's hydrophobicity makes it prone to aggregation. To improve its solubility and stability, nanomaterials with large surface areas and high chemical reactivity can be introduced as photocarriers. Cs3EuCl6 nanocrystals, as a lead-free rare earth-based perovskite material, have a high photoluminescence quantum yield (over 90%) and can convert ultraviolet light into red light. Carrying Ce6 on the Cs3EuCl6 nanocrystal carrier not only improves the stability of Ce6, but also forms an efficient energy transfer mechanism, realizing the efficient transfer of triplet energy, thereby significantly improving the efficiency of Ce6 in generating reactive oxygen species under light. On this basis, NO donors, such as arginine, are further introduced to efficiently stimulate the NO donor to release NO gas through a cascade reaction, forming a NO atmosphere protection and achieving a contactless antibacterial effect.

[0004] Fresh chicken is widely popular for its delicious flavor, delicate texture, and rich nutritional value, but its short shelf life, typically only two days, limits its market distribution and sales. Although ultraviolet (UV) irradiation can inhibit the growth of pathogenic microorganisms on the surface of chicken, prolonged UV exposure can lead to oxidation of fat and myoglobin in the chicken, potentially causing water loss, affecting the quality and market value of the chicken. Traditional contact meat preservatives, such as sodium lactate, potassium sorbate, and sodium citrate, do have significant antibacterial properties, but long-term use can lead to bacterial resistance. Cold-pressed corn germ oil is highly valued for its rich nutritional content, including carotenoids and polyphenols, but these components also make it highly susceptible to oxidation, causing rapid deterioration of the oil's quality. Traditional preservation methods (such as adding preservatives and refrigeration) are unlikely to effectively extend its shelf life, and direct UV exposure can accelerate the oxidation process, affecting the oil's flavor and nutritional value. Therefore, seeking efficient, safe and environmentally friendly non-contact food antibacterial preservation methods to inhibit bacterial invasion, reduce decay and prevent oxidative deterioration has important academic and practical value for ensuring the storage quality of food and extending its shelf life. Summary of the Invention

[0005] To address the numerous limitations of existing direct-contact food preservation technologies, the present invention proposes an innovative food antibacterial cling film based on NO atmosphere protection. The core of this design lies in its non-contact antibacterial mechanism. Under UV light, the NO-generating photosensitizer Cs3EuCl6-Ce6 in the NO atmosphere-protected food antibacterial cling film, through a cascade reaction, efficiently generates reactive oxygen species. This further stimulates arginine to produce a NO atmosphere, ultimately achieving its antibacterial and preservation effects without direct contact with the food. This maximizes the protection of the food's original flavor and nutritional content from external factors, while also avoiding the potential for bacterial resistance and food contamination that could result from direct contact.

[0006] The purpose of the present invention can be achieved by the following technical solutions:

[0007] One of the purposes of the present invention is to provide a NO-generating photosensitizer Cs3EuCl6-Ce6 based on a cascade reaction, which specifically includes a nanocrystal carrier Cs3EuCl6 and a photosensitizer Ce6 carried by it, wherein the mass ratio of the Cs3EuCl6 nanocrystal carrier to the photosensitizer Ce6 is 1:1~10.

[0008] Preferably, the excitation spectrum of the Cs3EuCl6 nanocrystals at 621 nm significantly overlaps with the excitation spectrum of Ce6 at 678 nm, indicating that the Cs3EuCl6 nanocrystals can effectively transfer energy to Ce6 molecules, thereby enhancing their phototoxicity. The average size of the Cs3EuCl6 nanocrystals ranges from 10 to 20 nm. After binding to Ce6, their morphology remains regular, with an average size range of 10 to 20 nm. This nanoscale size control further helps improve the water solubility and photostability of Ce6, thereby enhancing its phototoxicity and antibacterial effects.

[0009] One of the purposes of the present invention is to provide a NO generating photosensitizer Cs3EuCl6-Ce6 based on a cascade reaction, the preparation method of which comprises the following steps:

[0010] S1. Ultrasonic dispersion of lead-free rare earth-based perovskite material Cs3EuCl6 nanocrystals in water;

[0011] S2. Cs3EuCl6 and Ce6 are stirred and cross-linked using a magnetic stirring heater, and the NO-generating photosensitizer Cs3EuCl6-Ce6 based on the cascade reaction is obtained after standing, centrifuging and washing.

[0012] Preferably, the heating temperature of the magnetic stirring heater is 0-70° C., and the heating time is 1-5 hours.

[0013] Preferably, the centrifugal speed is 4000-13000 rpm / min, and the centrifugal time is 4-20 min.

[0014] Preferably, the mass ratio of Cs3EuCl6 to Ce6 is 1:1-10.

[0015] The second object of the present invention is to provide a method for preparing food antibacterial fresh-keeping film based on NO atmosphere protection.

[0016] The food antibacterial fresh-keeping film based on NO atmosphere protection used in the present invention is prepared from a NO-generating photosensitizer Cs3EuCl6-Ce6 based on a cascade reaction, an NO donor arginine, and a sodium carboxymethyl cellulose (CMC) substrate. The preparation method specifically includes the following steps:

[0017] Arginine was dissolved in deionized water, stirred, and mixed with the CMC solution to obtain a mixture; Cs3EuCl6-Ce6 nanocrystals were added, stirred, and poured onto a glass plate. After drying, the food antibacterial cling film based on NO atmosphere protection was obtained.

[0018] Preferably, the mass ratio of the arginine to water is 1:20-200.

[0019] Preferably, the mass ratio of the cascade-reaction-based NO-generating photosensitizer Cs3EuCl6-Ce6 to the CMC solution is 1:5-20.

[0020] Preferably, the concentration of the CMC solution is 0.001 to 0.5 mg / mL.

[0021] Preferably, the mixing time is 0.1 to 12 hours.

[0022] Preferably, the drying temperature is 10-50° C., and the drying time is 12-48 hours.

[0023] The third object of the present invention is to provide a food antibacterial fresh-keeping film based on NO atmosphere protection for use in inhibiting bacterial growth.

[0024] Compared to existing technologies, this invention uses Cs3EuCl6 nanocrystals as a carrier and carries the photosensitizer Ce6, thereby enhancing its phototoxicity and antibacterial effects. Under ultraviolet light, a cascade reaction efficiently stimulates arginine in the film to produce NO gas. Without direct contact with the precursor, it has a strong bactericidal effect against bacteria such as Staphylococcus aureus and Escherichia coli.

[0025] A fourth object of the present invention is to provide an application of a food antibacterial fresh-keeping film based on NO atmosphere protection in the preservation of fresh food.

[0026] The NO atmosphere-protected food antibacterial cling film used in this invention exhibits strong airborne antibacterial properties and can be used to preserve fresh food. Under ultraviolet light, a protective NO atmosphere forms around the food, achieving contactless antibacterial preservation. Unlike traditional antibacterial materials that may introduce contaminants such as microplastics, microbial contaminants, and heavy metals, this NO atmosphere-protected food antibacterial cling film reduces health risks and provides a safer antibacterial and fresh-keeping solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 Transmission electron micrograph of the NO-generating photosensitizer Cs3EuCl6-Ce6 based on a cascade reaction.

[0028] Figure 2 This is a scanning electron microscope photo of food antibacterial cling film protected by NO atmosphere.

[0029] Figure 3 The excitation spectrum of Cs3EuCl6 nanocrystals at 621 nm overlaps with the excitation spectrum of Ce6 at 678 nm.

[0030] Figure 4 These are curves showing the relationship between singlet oxygen generated by Cs3EuCl6 nanocrystals, cascade-reaction-based NO generation photosensitizer Cs3EuCl6-Ce6, and Ce6 under ultraviolet light and time.

[0031] Figure 5 The curves show the relationship between the NO produced by pure arginine film, photosensitizer Cs3EuCl6-Ce6 film, and food antibacterial fresh-keeping film based on NO atmosphere protection and time under ultraviolet light irradiation.

[0032] Figure 6 The antibacterial effect of food antibacterial plastic wrap based on NO atmosphere protection against Staphylococcus aureus and Escherichia coli.

[0033] Figure 7 The application effect of food antibacterial cling film based on NO atmosphere protection in chicken preservation.

[0034] Figure 8 The application effect of food antibacterial cling film based on NO atmosphere protection in the preservation of corn germ oil. DETAILED DESCRIPTION

[0035] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0036] A NO-generating photosensitizer Cs3EuCl6-Ce6 based on a cascade reaction, which specifically includes a nanocrystal carrier Cs3EuCl6 and a photosensitizer Ce6 carried by the nanocrystal carrier, wherein the mass ratio of the Cs3EuCl6 nanocrystal carrier to the photosensitizer Ce6 is 1:1-10.

[0037] As an embodiment of the preferred embodiment, the NO-generating photosensitizer Cs3EuCl6-Ce6, based on a cascade reaction, can generate reactive oxygen species under ultraviolet light irradiation. Furthermore, the average size of the Cs3EuCl6 nanocrystals is 10-20 nm. After combining with Ce6, their morphology remains regular, with an average size of 10-20 nm.

[0038] A cascade reaction-based NO-generating photosensitizer Cs3EuCl6-Ce6, the preparation method of which comprises the following steps:

[0039] S1. Ultrasonic dispersion of lead-free rare earth-based perovskite material Cs3EuCl6 nanocrystals in water;

[0040] S2. Cs3EuCl6 and Ce6 are stirred and cross-linked using a magnetic stirring heater, and the NO-generating photosensitizer Cs3EuCl6-Ce6 based on the cascade reaction is obtained after standing, centrifuging and washing.

[0041] The heating temperature of the magnetic stirring heater is 0-70° C., and the heating time is 1-5 hours.

[0042] The centrifugal speed is 4000-13000 rpm / min, and the centrifugal time is 4-20 min.

[0043] The mass ratio of Cs3EuCl6 to Ce6 is 1:1-10.

[0044] A method for preparing a food antibacterial fresh-keeping film based on NO atmosphere protection, the preparation method specifically comprising the following steps:

[0045] S1. Arginine was dissolved in deionized water, stirred, and mixed with the CMC solution to obtain a mixture;

[0046] S2. Add the NO-generating photosensitizer Cs3EuCl6-Ce6 nanocrystals based on the cascade reaction, stir and pour onto a glass plate, and after drying, obtain the food antibacterial fresh-keeping film based on NO atmosphere protection.

[0047] The mass ratio of arginine to water is 1:20-200.

[0048] The mass ratio of the NO generating photosensitizer Cs3EuCl6-Ce6 based on the cascade reaction to the CMC solution is 1:5-20.

[0049] The concentration of the CMC solution is 0.001-0.5 mg / mL.

[0050] The mixing time is 0.1 to 12 hours.

[0051] The drying temperature is 10-50°C and the drying time is 12-48 hours.

[0052] Application of food antibacterial cling film based on NO atmosphere protection in inhibiting bacterial growth.

[0053] The particle size distribution of the Cs3EuCl6-Ce6 photosensitizer, a cascade-induced NO-generating agent, in this paper's novel NO-protected food wrap has been reported to be 10 to 20 nm. Under UV light irradiation, the Cs3EuCl6 nanocrystals efficiently transfer energy to Ce6 molecules, stimulating them to produce reactive oxygen species and simultaneously triggering the release of 1.1 μM NO from arginine. Given the antibacterial properties of NO, this material has the potential to be used for bacterial inhibition.

[0054] Application of food antibacterial cling film based on NO atmosphere protection in fresh food preservation.

[0055] The present invention provides a food antibacterial cling film based on NO atmosphere protection, featuring a simple preparation process and low phototoxicity. It releases 1.1 μM NO under ultraviolet light. Furthermore, the film utilizes a non-contact antibacterial mechanism, forming a protective NO atmosphere around the food, achieving non-contact protection. These properties make it suitable for applications such as contactless antibacterial and fresh food preservation.

[0056] The present invention will be further described in detail below with reference to specific examples. The following examples are not intended to limit the present invention but are merely intended to illustrate the present invention. The experimental methods used in the following examples are generally based on conventional conditions unless otherwise specified. The materials and reagents used in the following examples are all commercially available unless otherwise specified.

[0057] Example 1

[0058] A method for preparing a food antibacterial fresh-keeping film based on NO atmosphere protection comprises the following steps:

[0059] The lead-free rare earth-based perovskite material Cs3EuCl6 nanocrystals were ultrasonically dispersed in water, and Cs3EuCl6 and Ce6 (1:2) were stirred and cross-linked using a magnetic stirring heater. After standing, centrifugation (10000 rpm / min, 8 min), and washing, the NO-generating photosensitizer Cs3EuCl6-Ce6 based on the cascade reaction was obtained. Arginine (2 g) was dissolved in deionized water (100 mL) and stirred, and then mixed with CMC solution (0.03 g / mL). The NO-generating photosensitizer Cs3EuCl6-Ce6 nanocrystals based on the cascade reaction were added, stirred for 1 hour, and then poured onto a glass plate to dry, to obtain a food antibacterial preservative film based on NO atmosphere protection. The transmission electron microscopy of the obtained NO-generating photosensitizer Cs3EuCl6-Ce6 based on the cascade reaction is as follows: Figure 1 As shown in the figure, it can be seen that the particle size of the NO-generating photosensitizer Cs3EuCl6-Ce6 based on the cascade reaction is 10-20nm. The scanning electron microscope of the food antibacterial preservative film based on NO atmosphere protection is as follows. Figure 2 As shown, the nanocrystals on the surface of the film are evenly distributed. The excitation spectrum of Cs3EuCl6 nanocrystals at 621nm and the excitation spectrum of Ce6 at 678nm are shown in Figure 3 As shown in the figure, there is a significant overlap between the two, indicating that under UV irradiation, Cs3EuCl6 nanocrystals can effectively transfer energy to Ce6 molecules, thereby enhancing their phototoxicity. Therefore, the theory of NO generation photosensitizer Cs3EuCl6-Ce6 based on cascade reaction is feasible.

[0060] The ability of Cs3EuCl6-Ce6 nanocrystals to generate reactive oxygen species under ultraviolet light was evaluated. TMB was added to a Cs3EuCl6-Ce6 nanocrystal solution as an active oxygen indicator. UV light was irradiated and the absorbance at 470 nm was measured. The results are shown in Figure 2. Figure 4 As shown, the absorbance value of the Cs3EuCl6-Ce6 nanocrystal solution increased significantly over time, indicating the efficient generation of reactive oxygen species, confirming its potential to stimulate NO generation in a cascade reaction.

[0061] The NO gas generation capacity of food antibacterial plastic wrap under NO atmosphere protection was measured using a Griess assay kit. Griess reagents I and II were mixed and added to a glass dish. The dish was sealed with antibacterial plastic wrap (5 mm from the liquid surface) and then irradiated with UV light. The absorbance at a wavelength of 540 nm was measured and the NO concentration was calculated using a standard curve. The results are shown in Figure 1. Figure 5 As shown in the figure, the food antibacterial wrap based on NO atmosphere protection has a significant NO gas production effect, reaching 1.1 μM.

[0062] The antibacterial effect was determined by sealing the food antibacterial plastic wrap based on NO atmosphere protection on Escherichia coli and Staphylococcus aureus evenly coated on solid culture medium, and then irradiating with ultraviolet light and culturing overnight. Figure 6 As shown in the experimental results, the treatment group using the food antibacterial cling film based on NO atmosphere protection and irradiated with ultraviolet light showed the most significant antibacterial effect (99.78% for Escherichia coli and 99.31% for Staphylococcus aureus), and had a highly efficient antibacterial effect in the air.

[0063] The application of food antibacterial plastic wrap based on NO atmosphere protection was explored using chicken models and corn germ oil models. In the chicken preservation application, fresh chicken breast was cut into pieces under sterile conditions, covered with food antibacterial plastic wrap based on NO atmosphere protection (5mm from the meat surface), and irradiated with ultraviolet light for 20 minutes every day at room temperature. The results were recorded. Figure 7 As shown, chicken treated with NO-based antibacterial plastic wrap retained excellent color and texture even after five days, significantly extending its shelf life. In a novel application for preserving corn germ oil, the oil was placed in an open container and covered with NO-based antibacterial plastic wrap (5 mm from the oil surface). The oil was then irradiated with UV light for 20 minutes daily at room temperature, and observations were recorded. The results showed that corn germ oil covered with NO-based antibacterial plastic wrap extended its shelf life and maintained excellent quality.

[0064] Example 2

[0065] The difference compared with Example 1 is that the preparation process of this embodiment is:

[0066] Lead-free rare earth perovskite material Cs3EuCl6 nanocrystals were ultrasonically dispersed in water (1.5 mg / mL). Cs3EuCl6 and Ce6 (1:3) were stirred and cross-linked using a magnetic stirring heater. After standing, centrifugation (9000 rpm / min, 8 minutes), and washing, the cascade reaction-based NO-generating photosensitizer Cs3EuCl6-Ce6 was obtained. Arginine (2.5 g) was dissolved in deionized water (100 mL) and stirred, then mixed with a CMC solution (0.03 g / mL). The cascade reaction-based NO-generating photosensitizer Cs3EuCl6-Ce6 nanocrystals were added, stirred for 1 hour, and then poured onto a glass plate. After drying, the food antibacterial preservative film based on NO atmosphere protection was obtained.

[0067] Example 3

[0068] The difference compared with Example 1 is that the preparation process of this embodiment is:

[0069] Lead-free rare earth perovskite material Cs3EuCl6 nanocrystals were ultrasonically dispersed in water (2 mg / mL). Cs3EuCl6 and Ce6 (1:3) were stirred and cross-linked using a magnetic stirring heater. The mixture was allowed to stand, centrifuged (8500 rpm / min, 8 minutes), and washed to obtain the NO-generating photosensitizer Cs3EuCl6-Ce6 based on a cascade reaction. Arginine (3 g) was dissolved in deionized water (110 mL) and stirred, then mixed with a CMC solution (0.03 g / mL). The NO-generating photosensitizer Cs3EuCl6-Ce6 nanocrystals based on a cascade reaction were added, stirred for 1.5 hours, and then poured onto a glass plate. After drying, the food antibacterial preservative film based on NO atmosphere protection was obtained.

[0070] Example 4

[0071] The difference compared with Example 1 is that the preparation process of this embodiment is:

[0072] Lead-free rare earth perovskite material Cs3EuCl6 nanocrystals were ultrasonically dispersed in water (2 mg / mL). Cs3EuCl6 and Ce6 (1:2) were stirred and cross-linked using a magnetic stirring heater. After standing, centrifugation (8000 rpm / min, 8 minutes), and washing, the cascade reaction-based NO-generating photosensitizer Cs3EuCl6-Ce6 was obtained. Arginine (3 g) was dissolved in deionized water (110 mL) and stirred, then mixed with CMC solution (0.03 g / mL). The cascade reaction-based NO-generating photosensitizer Cs3EuCl6-Ce6 nanocrystals were added, stirred for 1.5 hours, and poured onto a glass plate. After drying, the food antibacterial preservative film based on NO atmosphere protection was obtained.

[0073] Example 5

[0074] The difference compared with Example 1 is that the preparation process of this embodiment is:

[0075] Lead-free rare earth perovskite material Cs3EuCl6 nanocrystals were ultrasonically dispersed in water (2 mg / mL). Cs3EuCl6 and Ce6 (1:2) were crosslinked using a magnetic stirring heater. The mixture was allowed to stand, centrifuged (9000 rpm / min, 8 minutes), and washed to obtain the NO-generating photosensitizer Cs3EuCl6-Ce6 based on a cascade reaction. Arginine (3 g) was dissolved in deionized water (120 mL) and stirred, then mixed with a CMC solution (0.03 g / mL). The NO-generating photosensitizer Cs3EuCl6-Ce6 nanocrystals based on a cascade reaction were added, stirred for 2 hours, and then poured onto a glass plate. After drying, the food antibacterial preservative film based on NO atmosphere protection was obtained.

[0076] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A method for preparing a NO generating photosensitizer Cs3EuCl6-Ce6 based on a cascade reaction, characterized in that: The preparation method comprises the following steps: S1. Ultrasonic dispersion of lead-free rare earth-based perovskite material Cs3EuCl6 nanocrystals in water; S2. Cs3EuCl6 and Ce6 are cross-linked by stirring with a magnetic stirring heater, and the NO-generating photosensitizer Cs3EuCl6-Ce6 based on the cascade reaction is obtained after standing, centrifuging and washing.

2. The preparation method according to claim 1, characterized in that: The heating temperature of the magnetic stirring heater is 0-70° C., and the heating time is 1-5 hours.

3. The preparation method according to claim 1, characterized in that: The mass ratio of Ce6 to Cs3EuCl6 is 1:0.1-10.

4. A method for preparing food antibacterial cling film based on NO atmosphere protection, characterized in that: The preparation method comprises the following steps: S1. Dissolve arginine in deionized water and mix with CMC solution after stirring; S2. Add Cs3EuCl6-Ce6 nanocrystals, a photosensitizer generated by NO based on a cascade reaction, stir and pour onto a glass plate to dry, and obtain a food antibacterial preservative film based on NO atmosphere protection.

5. The preparation method according to claim 4, characterized in that: The mass ratio of the arginine to water is 1:20-200.

6. The preparation method according to claim 4, characterized in that: The mass ratio of the NO generating photosensitizer Cs3EuCl6-Ce6 based on the cascade reaction to the CMC solution is 1:5-20.

7. The preparation method according to claim 4, characterized in that: The concentration of the CMC solution is 0.001-0.5 mg / mL.

8. The preparation method according to claim 4, characterized in that: The drying temperature is 10-50°C and the drying time is 12-48h.

9. Application of the food antibacterial fresh-keeping film based on NO atmosphere protection prepared by the preparation method according to any one of claims 1 to 8 in inhibiting bacteria.

10. Use of the food antibacterial fresh-keeping film based on NO atmosphere protection prepared by the preparation method according to any one of claims 1 to 8 in the preservation of fresh food.

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