Trichoderma virens strain KN-T01, trichoderma preparation and application of trichoderma virens strain KN-T01

Abstract

The invention discloses a trichoderma virens strain KN-T01, a trichoderma preparation and application of the trichoderma preparation, and belongs to the technical field of biocontrol microbial agents. The invention provides a trichoderma virens strain KN-T01, a trichoderma preparation and application of the trichoderma virens strain KN-T01 and aims to solve the technical problem that a biocontrol microbial agent in the prior art is poor in microbial function effect in a low-temperature environment, the preservation number of the trichoderma virens strain KN-T01 is CCTCC M 20242443, and the trichoderma preparation contains the trichoderma virens strain KN-T01. The trichoderma virens strain KN-T01 and the trichoderma preparation provided by the invention have relatively good low temperature resistance, have relatively good antagonism and hyperparasitism effects on pathogenic bacteria of pepper gray mold and watermelon fusarium wilt, have a good decomposition effect on straws under a low-temperature condition, and have a relatively good promotion effect on growth of tomatoes and corns under a low-temperature condition, so that the trichoderma virens strain KN-T01 and the trichoderma preparation have relatively good application prospects. The trichoderma virens strain KN-T01 and the trichoderma preparation are non-toxic and harmless, and can be applied to agricultural green prevention and control.

Description

Technical Field

[0001] The invention belongs to the technical field of biocontrol microbial agents, and specifically relates to a Trichoderma viride strain, a Trichoderma preparation and applications thereof. Background Art

[0002] Temperature is closely related to plant growth and development. Low temperature stress is a non-biological stress and one of the main environmental factors that restrict plant growth and development and microbial growth. In recent years, the global climate has been changeable, and the frequency of extreme weather has increased. Crops have suffered from low temperature stress, such as the increased probability of late spring cold and the increased degree of damage. When crops encounter low temperatures, the growth rate will be significantly slowed down, and they may also suffer from low temperature chilling and frost damage, which seriously affects the yield and quality of crops.

[0003] In recent years, with the continuous progress of agricultural science and technology in my country, the production of grain crops such as wheat, corn, and rice has increased year by year. With the popularization of mechanization, a large amount of straw is left in the field. Under the policy of prohibiting burning, its treatment has become a difficult problem that needs to be solved urgently. Under suitable temperature, the organisms in the field or the artificial addition of composting agents can gradually decompose and compost it. In a low temperature environment, most microorganisms are in a dormant state, the biological activity is insufficient, and the composting speed of the straw is extremely slow; uncomposted straw not only cannot provide effective nutrients for the soil, but may also affect the sowing and growth of the next crop; and the traditional straw composting method is not effective at low temperatures and requires a lot of time and labor costs.

[0004] As representative leaf and soil-borne diseases, pepper gray mold and watermelon wilt are currently global epidemics. Once they start to occur, they spread rapidly and are difficult to control. They are very difficult to prevent and control, and have a great impact on the growth of crops, and will also seriously harm the yield and quality of crops. At present, conventional prevention and control still uses a large amount of chemical agents, and excessive use of chemical pesticides will cause damage to the soil and ecological environment, and pests and diseases will also develop drug resistance, making long-term prevention and control more difficult.

[0005] In recent years, biopesticides have been widely used for their safety, high efficiency, environmental friendliness, harmlessness to humans and animals, and resistance to pests and diseases. As the main force of biocontrol fungi, Trichoderma occupies an important position in the field of biopesticides. Its biocontrol efficacy and growth-promoting function are well known, but there are few reports on its low-temperature application. Therefore, those skilled in the art are eager to develop a biocontrol microbial agent that is safer, environmentally friendly, not prone to resistance, and can grow normally in a low-temperature environment to exert its microbial function. Summary of the invention

[0006] The present invention aims to solve the technical problem that the biocontrol microbial agent in the prior art has poor microbial function effect in a low temperature environment, and provides a Trichoderma viride strain KN-T01, a Trichoderma preparation and application thereof.

[0007] One of the objects of the present invention is to provide a Trichoderma virens strain KN-T01, the Trichoderma virens strain KN-T01 has a preservation number of CCTCC M 20242443, is classified as Trichoderma virens, and is preserved in the China Center for Type Culture Collection, with a preservation date of November 6, 2024.

[0008] The second object of the present invention is to provide a Trichoderma preparation, which contains the above-mentioned Trichoderma viride strain KN-T01 or spore powder of Trichoderma viride strain KN-T01.

[0009] In a preferred embodiment of the present invention, the Trichoderma preparation is a wettable powder.

[0010] In a preferred embodiment of the present invention, when the Trichoderma preparation is a wettable powder, the Trichoderma wettable powder further comprises sodium lignin sulfonate, D-425, EFW, trehalose and sodium gluconate, and one or more of chitosan, cellulose and tapioca dextrin.

[0011] In a preferred embodiment of the present invention, the mass percentage composition of the Trichoderma wettable powder is: 10-15% spore powder of Trichoderma viride strain KN-T01, 1-2% sodium lignin sulfonate or D-425, 1-2% EFW, 20-30% trehalose, 2% in total of one or more of chitosan, cellulose, and tapioca dextrin, and 49-66% sodium gluconate; the spore content of the Trichoderma viride strain KN-T01 spore powder is ≥2 billion spores / g.

[0012] In a preferred embodiment of the present invention, the method for preparing the spore powder of Trichoderma viride strain KN-T01 comprises the following steps:

[0013] S1: After activating the above-mentioned Trichoderma viride KN-T01, pick out the bacterial blocks and inoculate them into PSB culture medium, and shake culture them at 26-28°C and 160rpm for 2-3 days to obtain a spore suspension; the concentration of Trichoderma viride KN-T01 spores in the spore suspension is 10 7 -10 8 spores / mL, the PSB culture solution formula is: 200g / L potato, 20g / L sucrose, and the balance is water;

[0014] S2: inoculating the spore suspension obtained in S1 onto a culture medium at a 20% inoculation rate, fermenting at 26-28° C. for 10-15 days, and drying for 2-3 days to obtain a solid fermentation product; the culture medium formula is: 200-300 g bran, 75-100 g corn flour, 10-20 g soybean meal, 5-10 g sucrose, 0.5-1 g magnesium sulfate, 0.5-1 g dipotassium hydrogen phosphate, and an initial water content of 40-60%;

[0015] S3: The solid fermentation product obtained in S2 is crushed to obtain spore powder of Trichoderma virens strain KN-T01.

[0016] The third object of the present invention is to provide the use of the above-mentioned Trichoderma viride strain KN-T01 or Trichoderma preparation in inhibiting pathogenic bacteria.

[0017] In a preferred embodiment of the present invention, the pathogen is the pathogen of watermelon wilt or the pathogen of pepper gray mold.

[0018] A fourth object of the present invention is to provide the use of the above-mentioned Trichoderma viride strain KN-T01 or Trichoderma preparation in promoting low-temperature composting of straw.

[0019] A fifth object of the present invention is to provide the use of the above-mentioned Trichoderma viride strain KN-T01 or Trichoderma preparation in promoting plant growth at low temperature, wherein the plants include tomatoes or corn.

[0020] Beneficial effects of the present invention: The present invention provides a green Trichoderma strain KN-T01, a Trichoderma preparation and its application. The green Trichoderma strain KN-T01 has a deposit number of CCTCC M 20242443. The Trichoderma preparation contains the green Trichoderma strain KN-T01. The green Trichoderma strain KN-T01 is obtained by separation and purification from a soil sample of a paddy field in Heilongjiang Province. It is safe and pollution-free to crops and soil. It has been verified that the strain has obvious growth vitality under low temperature conditions of 6-8°C and has good low temperature resistance. The present invention uses spore powder of the green Trichoderma strain KN-T01, and cooperates with sodium lignin sulfonate, D-425, EFW, trehalose and sodium gluconate, as well as one or more of chitin, cellulose and tapioca dextrin to prepare the Trichoderma preparation.

[0021] Under low temperature conditions, wheat straw was treated with Trichoderma viride strain KN-T01, and it was found that the weight loss rate after treatment reached 27.73%, indicating that the Trichoderma viride strain KN-T01 has an obvious decomposition effect at low temperature and has a good low-temperature decomposition effect.

[0022] The green Trichoderma strain KN-T01 provided by the invention has good antagonism and hyperparasitism against pepper gray mold pathogens and watermelon wilt pathogens, wherein the antibacterial effects against pepper gray mold pathogens and watermelon wilt pathogens are as high as 70.6% and 70% respectively.

[0023] The green Trichoderma strain KN-T01 and the Trichoderma preparation thereof provided by the present invention have a good promoting effect on the growth of tomato and corn seedlings under low temperature. Compared with the control group, the tomato treated with the green Trichoderma strain KN-T01 has a plant height increase of 10.16%, a stem diameter increase of 11.87%, a fresh weight of the aboveground part increase of 24.01%, a root length increase of 19.02%, and a root weight increase of 30.32%; the corn germination rate after seed dressing treatment with the Trichoderma preparation containing the green Trichoderma strain KN-T01 increases by 12.16%, a plant height increase of 6.15%, a stem base width increase of 4.32%, a root length increase of 6.12%, a stem and leaf fresh weight increase of 10.53%, and a root fresh weight increase of 10.64%; it can be seen that the green Trichoderma strain KN-T01 and the Trichoderma preparation thereof have a good effect of promoting plant growth under low temperature conditions.

[0024] The Trichoderma preparation containing the green Trichoderma strain KN-T01 provided by the present invention can be used as a seed treatment agent for seed dressing, can be used for foliar spray treatment to prevent and control leaf diseases, can be used for root irrigation treatment to prevent and control soil-borne diseases and promote crop growth, is non-toxic and harmless, is environmentally friendly, and can be applied to agricultural green prevention and control.

[0025] [Biological preservation information]: The Trichoderma virens strain is named KN-T01. The preservation number of the Trichoderma virens strain KN-T01 is CCTCC M 20242443, and the classification name is Trichoderma virens. It is preserved in the China Center for Type Culture Collection in Wuhan, and the preservation date is November 6, 2024. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The phylogenetic tree of the RPB2 region sequence of the Trichoderma viride species in Example 1;

[0027] Figure 2 The phylogenetic tree of the TEF1 region sequence of the Trichoderma viride species in Example 1;

[0028] Figure 3 The morphology and microscopic examination diagram of the Trichoderma viride strain KN-T01 in Example 1; a is a morphological diagram, and b is a microscopic examination diagram;

[0029] Figure 4 1 is a graph showing the inhibitory effect of the Trichoderma viride strain KN-T01 in Example 2; a is a graph showing the inhibitory effect of Trichoderma viride KN-T01 on pepper gray mold pathogen, and b is a graph showing the inhibitory effect of Trichoderma viride KN-T01 on watermelon wilt pathogen;

[0030] Figure 5 The graph is a graph showing the growth-promoting effect of the Trichoderma viride strain KN-T01 on tomato seedlings in Example 4; a is the control group, and b is the Trichoderma viride strain KN-T01 treatment group;

[0031] Figure 6 The graph is a diagram showing the effect of Trichoderma viride strain KN-T01 on low temperature ripening of straw in Example 5; a is the control group, and b is the Trichoderma viride strain KN-T01 treatment group;

[0032] Figure 7 This is a diagram showing the effect of the Trichoderma preparation containing the Trichoderma strain KN-T01 in Example 6 on low-temperature emergence of corn in the field after seed dressing; a is the effect diagram of the group treated with the Trichoderma strain KN-T01, and b is the effect diagram of the control group. DETAILED DESCRIPTION

[0033] Those skilled in the art can refer to the content of this article and appropriately improve the process parameters. It should be particularly noted that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content and scope of the present invention to implement and apply the technology of the present invention.

[0034] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments. The test methods used in the following examples are all conventional methods unless otherwise specified, and the materials, reagents, methods and instruments used are conventional materials, reagents, methods and instruments in the art unless otherwise specified, and those skilled in the art can obtain them through commercial channels.

[0035] The Trichoderma DJ described in the following examples is Trichoderma harzianum, which was purchased from Dajing Biological and has a trade name of Wangtaibao.

[0036] The wettable powder of Trichoderma viride strain KN-T01 described in the following examples is the Trichoderma preparation described in the present invention.

[0037] The composition of the PSA plate described in the following examples is: 200 g / L potato, 20 g / L sucrose, 20 g / L agar, and the balance is water.

[0038] The PSB culture medium is composed of 200 g / L potato, 20 g / L sucrose, and the balance is water.

[0039] Example 1: Identification and cultivation of Trichoderma virens strain KN-T01

[0040] In this example, the Trichoderma viride strain KN-T01 isolated and purified from a paddy field soil sample in Heilongjiang Province was preserved with the preservation number of CCTCC M 20242443, and the species was identified. The results showed that the RPB2 region sequence of the Trichoderma viride strain KN-T01 was as shown in SEQ ID NO.1, and the TEF1 region sequence of the Trichoderma viride strain KN-T01 was as shown in SEQ ID NO.2.

[0041] In this example, the RPB2 region sequence as shown in SEQ ID NO.1 was compared with the RPB2 region sequence of the model bacteria of the genus Trichoderma that has been published to construct a phylogenetic tree. Figure 1 As shown; and the TEF1 region sequence measured as shown in SEQ ID NO.2 and the TEF1 region sequence of the similar model strain in the genus Trichoderma were used to construct a phylogenetic tree, as shown in Figure 2 As shown; it can be seen that the RPB2 and TEF1 region sequences of the Trichoderma virens strain KN-T01 provided by the present invention are closely related to the RPB2 and TEF1 region sequences of the published model bacteria of the genus Trichoderma. The Trichoderma virens strain KN-T01 provided by the present invention is identified as a Trichoderma virens strain.

[0042] In this example, the green Trichoderma strain KN-T01 provided by the present invention was cultured on a PDA medium. It was observed that the green Trichoderma strain KN-T01 grew rapidly, with dense aerial hyphae and a large number of spore-producing clusters forming green concentric rings (such as Figure 3 The conidiophores are translucent, with irregular branches toward the top. Each branch has 3-6 closely attached phialides at the top. The phialides are jar-shaped or ampoule-shaped, constricted at the base, swollen in the middle, and gradually tapering toward the top. Conidia are elliptical (as shown in Figure 1). Figure 3 (as shown in b).

[0043] Example 2: Inhibitory effect of Trichoderma virens strain KN-T01 on pathogenic bacteria

[0044] The pathogens used in this embodiment include: the pathogen of pepper gray mold is Botrytis cinerea, and the pathogen of watermelon wilt is Fusarium oxysporum citronellal type, and the above strains are all from the Institute of Plant Protection and Soil and Fertilizer of Hubei Academy of Agricultural Sciences.

[0045] Test method:

[0046] (1) Activation of strains: Take the Trichoderma viride strain KN-T01, the pathogen of pepper gray mold, and the pathogen of watermelon wilt stored in the ultra-low temperature refrigerator, inoculate them on PSA plates respectively, and incubate them upside down in a constant temperature incubator at 26-28° C. for 5-7 days to obtain activated strains of Trichoderma viride strain KN-T01, the pathogen of pepper gray mold, and the pathogen of watermelon wilt;

[0047] (2) Plate confrontation: Use a hole puncher to poke out 5 mm diameter activated Trichoderma viride strain KN-T01, pepper gray mold pathogen and watermelon wilt pathogen cakes, place them in symmetrical positions on both sides of the PSA plate, and then put them in a 26°C constant temperature incubator for 7-10 days. The antibacterial rate of Trichoderma viride strain KN-T01 against pepper gray mold pathogen and watermelon wilt pathogen is:

[0048]

[0049] The results are as follows Figure 4 As shown in Table 1, the green Trichoderma strain KN-T01 provided by the present invention has a significant inhibitory effect on the growth of pepper gray mold and watermelon wilt pathogens, and the subsequent pathogen surface is covered with Trichoderma hyphae and spores, which shows that the green Trichoderma strain KN-T01 has an obvious hyperparasitism phenomenon.

[0050] Table 1

[0051]

[0052]

[0053] Example 3: Preparation of Trichoderma virens strain KN-T01 wettable powder

[0054] (1) Activation of strains: Take the Trichoderma viride KN-T01 strain stored in an ultra-low temperature refrigerator, inoculate it on a PSA plate, and incubate it upside down in a constant temperature incubator at 26-28° C. for 3-5 days to obtain an activated strain of Trichoderma viride KN-T01;

[0055] (2) Preparation of seed solution: The activated Trichoderma viride strain KN-T01 in (1) was inoculated into the sterilized PSB medium, placed in a constant temperature shaker, the temperature was controlled at 26-28° C., the shaker speed was 160 rpm, and the liquid fermentation was performed for 2-3 days to obtain the Trichoderma viride strain KN-T01 seed solution;

[0056] (3) Trichoderma solid fermentation: Weigh 200 g of bran, 100 g of corn flour, 20 g of soybean meal, 10 g of sucrose, 0.5 g of magnesium sulfate, and 1 g of potassium dihydrogen phosphate, with an initial water content of 60%, stir and mix, sterilize in an autoclave at 121° C. for 30 minutes, take out and cool to obtain a culture medium; inoculate the green Trichoderma strain KN-T01 seed liquid obtained in (2) into the above culture medium at a 20% inoculation rate, stir evenly, and place in a constant temperature incubator at 26-28° C. for 10-15 days, and wait until it turns green to obtain a solid fermentation product;

[0057] (4) Collection of spore powder: The solid fermentation obtained in (3) is placed in an oven at below 40° C. for drying or naturally dried for 2-3 days, and the spore powder is collected by a cyclone separator to obtain spore powder of Trichoderma viride strain KN-T01;

[0058] (5) Preparation of wettable powder: 10% spore powder of Trichoderma viride KN-T01 obtained in (4), 2% chitosan + cellulose + tapioca dextrin, 2% sodium lignin sulfonate, 2% EFW, 20% trehalose, 64% sodium gluconate, and the raw materials are weighed according to the above formula and mixed thoroughly to obtain a wettable powder of Trichoderma viride KN-T01.

[0059] Example 4: Effect of Trichoderma virens strain KN-T01 on growth promotion of tomato seedlings at low temperature

[0060] Test method:

[0061] (1) Activation of strains: The Trichoderma viride KN-T01 strain stored in an ultra-low temperature refrigerator was inoculated on a PSA plate and incubated in an incubator at 26-28°C for 5-7 days. The plate covered with Trichoderma viride spores was then rinsed with sterile water, and the rinsed liquid was collected and filtered through a layer of lens paper to obtain a Trichoderma viride KN-T01 spore suspension. The spore concentration was adjusted to 5×10 after counting using a hemocytometer. 5 cfu / mL;

[0062] (2) Tomato seedlings that have grown to the two-leaf and one-heart stage were selected as the test objects. Tomato seedlings of uniform size, free of pests and diseases, and without mechanical damage were selected and transplanted into flower pots. The flower pots were filled with an equal amount of substrate soil (organic matter content > 20%). After transplanting, the seedlings were allowed to grow for one week before use.

[0063] (3) After the seedling acclimatization in (2) was completed, the tomato seedlings were divided into two groups. The treatment group was watered with the spore suspension of Trichoderma viride strain KN-T01 obtained in (1) at a dosage of 50 ml / pot once a week. The tomato seedlings watered with clear water served as the control group. Each treatment group had 15 pots, and the process was repeated 3 times.

[0064] (4) The tomato seedlings after the above treatment were placed in a low temperature environment of 8-16°C for 20 days, and samples were taken from the tomato seedlings after cultivation to measure the plant growth indicators of the treatment group and the control group, including: plant height, stem diameter, root length, stem and leaf fresh weight, and root fresh weight.

[0065] The results are as follows Figure 5As shown in Table 2, compared with the control group, the tomato plant height treated with the Trichoderma viride strain KN-T01 provided by the present invention increased by 10.16%, the stem diameter increased by 11.87%, the aboveground fresh weight increased by 24.01%, the root length increased by 19.02%, and the root weight increased by 30.32%; it can be seen that the Trichoderma viride strain KN-T01 provided by the present invention has a good promoting effect on the growth of tomato seedlings under low temperature.

[0066] Table 2

[0067]

[0068] Example 5: Effect of Trichoderma viride strain KN-T01 on low temperature ripening of wheat straw

[0069] Test method:

[0070] (1) Activation of strains: Take the Trichoderma viride KN-T01 strain and the Trichoderma harzianum DJ strain stored in the ultra-low temperature refrigerator, inoculate them on PSA plates, and culture them upside down in a constant temperature incubator at 26-28° C. for 3-5 days to obtain the activated strains of Trichoderma viride KN-T01 strain and Trichoderma harzianum DJ strain, respectively;

[0071] (2) Seed liquid preparation: Pick the activated Trichoderma viride strain KN-T01 and Trichoderma harzianum DJ bacterial blocks in (1), inoculate them into the sterilized PSB medium, put them into a constant temperature shaker, and ferment them in liquid at 26-28°C and 160 rpm for 2-3 days to obtain Trichoderma viride strain KN-T01 and Trichoderma harzianum DJ seed liquid respectively;

[0072] (3) Low-temperature composting: Cut wheat straw into small pieces of 3-5 cm in diameter and place in an oven at 85°C to dry for 12 h. Weigh 1000 g of dried straw and 1000 g of water, stir and mix, then add 500 g of the seed liquid of Trichoderma viride strain KN-T01 and Trichoderma harzianum DJ obtained in (2). The treatment group with added clean water is used as the control group. Stir and mix again, and place in an incubator at 8°C for 14 days. Take out the straw after 14 days of composting, rinse it with water on a 60-mesh sieve until the eluent is clear and colorless, dry the remaining straw in an oven at 105°C for more than 12 h to constant weight, and weigh the weight of the remaining straw.

[0073] The statistical results of straw weight loss are shown in Table 3. Under low temperature conditions, the weight loss rate of the straw treated with Trichoderma viride strain KN-T01 was 27.73%, while the weight loss rate of the straw treated with Trichoderma harzianum DJ was only 7.08%. The weight loss rate of the straw treated with Trichoderma viride strain KN-T01 was increased by 20.65% compared with that treated with Trichoderma harzianum DJ. This shows that the Trichoderma viride strain KN-T01 provided by the present invention has obvious composting effect and its composting effect is better.

[0074] Table 3

[0075] Straw weight before treatment (g) Weight of treated straw (g) Weight loss rate (%) Water control group 1000 976.2 2.38 Trichoderma virens KN-T01 1000 722.7 27.73 Trichoderma harzianum DJ 1000 929.2 7.08

[0076] Example 6: Effect of Trichoderma virens strain KN-T01 wettable powder on growth promotion of corn seedlings at low temperature

[0077] In this embodiment, the Fujin area of ​​Heilongjiang Province is in a period of low night temperature in early May, with nighttime temperatures generally below 10°C and daytime temperatures averaging 16-18°C, as a typical low-temperature application scenario. The effect experiment was conducted according to the medication scheme in Table 4, and the corn seeds were treated with the green Trichoderma strain KN-T01 wettable powder prepared in Example 3. The corn seeds were treated with the seed, water, and drug ratio of 500:50:1. The control group was not treated with the green Trichoderma strain KN-T01 wettable powder. After seed dressing, 5,000 seeds per mu were sown and managed normally. The emergence time, emergence rate, plant height, stem thickness, root length, stem and leaf fresh weight, and root fresh weight of the corn seedlings were counted.

[0078] The results are shown in Table 5. Compared with the control group, the emergence time of corn seeds treated with the wettable powder of Trichoderma viride strain KN-T01 provided by the present invention was advanced by 1 day, the emergence rate increased by 12.16%, the plant height increased by 6.15%, the stem base width increased by 4.32%, the root length increased by 6.12%, the stem and leaf fresh weight increased by 10.53%, and the root fresh weight increased by 10.64%; it can be seen that the corn seedlings treated with the wettable powder of Trichoderma viride strain KN-T01 emerged early, the seedlings were relatively strong, and the growth was good; indicating that the wettable powder of Trichoderma viride strain KN-T01 provided by the present invention has a good promoting effect on the growth of corn under low temperature.

[0079] Table 4

[0080] Group Medication regimen Control group General treatment KN-T01 strain wettable powder Seed dressing of corn seeds with Trichoderma viride KN-T01 WP

[0081] Table 5

[0082]

[0083] The contents not described in detail in the specification of the present invention are well-known technologies for those skilled in the art. Although the present invention has been disclosed as above with preferred embodiments, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention should be based on the definition of the claims.

Claims

1. A Trichoderma viride strain KN-T01, characterized in that The green Trichoderma strain KN-T01 has a preservation number of CCTCC M20242443, is classified as Trichoderma virens, and is preserved in the China Center for Type Culture Collection, with a preservation date of November 6, 2024.

2. A Trichoderma preparation, characterized in that The Trichoderma preparation contains the Trichoderma viride strain KN-T01 according to claim 1 or spore powder of the Trichoderma viride strain KN-T01.

3. The Trichoderma preparation according to claim 2, characterized in that The Trichoderma preparation is a wettable powder.

4. The Trichoderma preparation according to claim 3, characterized in that When the Trichoderma preparation is a wettable powder, the Trichoderma wettable powder further comprises sodium lignin sulfonate, D-425, EFW, trehalose and sodium gluconate, and one or more of chitin, cellulose and tapioca dextrin.

5. The Trichoderma preparation according to claim 4, characterized in that The mass percentage composition of the Trichoderma wettable powder is: 10-15% of the spore powder of the green Trichoderma strain KN-T01, 1-2% of sodium lignin sulfonate or D-425, 1-2% of EFW, 20-30% of trehalose, 2% in total of one or more of chitin, cellulose, and tapioca dextrin, and 49-66% of sodium gluconate; the spore content of the green Trichoderma strain KN-T01 spore powder is ≥2 billion spores / g.

6. The Trichoderma preparation according to claim 2, characterized in that The method for preparing the spore powder of Trichoderma viride strain KN-T01 comprises the following steps: S1: After activating the Trichoderma viride KN-T01 strain according to claim 1, pick out the bacterial block and inoculate it into PSB culture medium, and shake culture it at 26-28°C and 160rpm for 2-3 days to obtain a spore suspension; the concentration of Trichoderma viride KN-T01 spores in the spore suspension is 10 7 -10 8 cfu / mL, the PSB culture solution formula is: 200g / L potato, 20g / L sucrose, and the balance is water; S2: inoculating the spore suspension obtained in S1 onto a culture medium at a 20% inoculation rate, fermenting at 26-28° C. for 10-15 days, and drying for 2-3 days to obtain a solid fermentation product; the culture medium formula is: 200-300 g bran, 75-100 g corn flour, 10-20 g soybean meal, 5-10 g sucrose, 0.5-1 g magnesium sulfate, 0.5-1 g dipotassium hydrogen phosphate, and an initial water content of 40-60%; S3: The solid fermentation product obtained in S2 is crushed to obtain spore powder of Trichoderma virens strain KN-T01.

7. Use of the Trichoderma viride strain KN-T01 according to claim 1 or the Trichoderma preparation according to any one of claims 2 to 6 in inhibiting pathogenic bacteria.

8. The use according to claim 7, characterized in that: The pathogen is a watermelon wilt pathogen or a pepper gray mold pathogen.

9. Use of the green Trichoderma strain KN-T01 described in claim 1 or the Trichoderma preparation described in any one of claims 2 to 6 in promoting low-temperature composting of straw.

10. Use of the Trichoderma viride strain KN-T01 according to claim 1 or the Trichoderma preparation according to any one of claims 2 to 6 in promoting plant growth at low temperatures, characterized in that: The plant includes tomato or corn.

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