Method for predicting human body exhaled gas

Abstract

The invention relates to a human body exhaled gas prediction method. The method comprises the following steps: acquiring target human body exhaled gas; inputting gas exhaled by the target human body into a flow-through medium path discharge source to output soft ionization gaseous molecules; inputting the soft ionization gaseous molecules into GC-MS equipment for ionization, and analyzing the input data after ionization through a carbon dioxide isotope internal standard quantitative method to obtain a prediction result; and outputting the prediction result and performing visual display. According to the method, the obtained target human body exhaled gas is subjected to result prediction based on the carbon dioxide isotope internal standard quantitative method through the flow-through medium channel discharge source and the GC-MS device which are connected in series, and on the basis that the whole process is high in real-time performance and easy to operate, the components of the human body exhaled gas can be rapidly and conveniently predicted.

Description

Technical Field

[0001] The present invention relates to the technical field related to gas composition prediction, and in particular to a method for predicting human exhaled gas. Background Art

[0002] Endogenous compounds in human exhaled breath, including inorganic gases (such as NO and CO) and VOCs (such as isoprene, ethane, pentane, acetone), are metabolites of human tissues. By analyzing the components and concentrations of endogenous markers, relevant information on human tissue metabolism and health status can be obtained non-invasively, which helps to clarify the corresponding physiological processes, disease occurrence, disease progression, and pharmacological responses.

[0003] The main disadvantages of the current breath diagnosis technology based on gas chromatography are the lack of dynamics and difficulty in quantification. Breathing is a dynamic process, and the analytical methods currently reported are mostly static, which makes quantitative determination difficult. The quantitative method and accurate determination of endogenous markers need further research. Summary of the invention

[0004] The purpose of the present invention is to solve at least one of the deficiencies of the prior art and to provide a method for predicting human exhaled gas.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions: Specifically, a method for predicting human exhaled gas is proposed, including the following: Acquire exhaled gas from a target human body; The target human exhaled gas is input into the flow-through medium channel discharge source to output soft ionized gas molecules; The soft ionized gaseous molecules are input into the GC-MS device for ionization, and the ionized input data are analyzed by the carbon dioxide isotope internal standard quantitative method to obtain the prediction results; The prediction results are output and visualized.

[0006] Further, specifically, the method of obtaining and acquiring the exhaled gas of the target human body includes oral exhalation and nasal exhalation, wherein, For oral exhaled sampling, you need to pinch your nose and blow air into a disposable straw only with your mouth. For nasal exhaled sampling, you need to hold a fixed device in your mouth, place the head of the disposable straw on the outside of your nostril, and exhale alternately through both nostrils.

[0007] Further, specifically, the acquired target human exhaled gas is directly set at the input end of the flow-through medium path discharge source, and the output end of the flow-through medium path discharge source is directly connected to the input end of the GC-MS device, that is, the flow-through medium path discharge source and the GC-MS device are integrated.

[0008] Furthermore, the method further comprises: When obtaining the exhaled gas of the target human body, the exhaled gas of the target human body will also be pre-processed, that is, a disposable filter head is installed at the front end of the disposable straw, and the disposable filter head is used to filter out suspended particles and water vapor in the exhaled gas of the human body.

[0009] Further, specifically, the ionized input data is analyzed by carbon dioxide isotope internal standard quantitative method to obtain prediction results, including: Determine the types of endogenous markers and the normal value range of each endogenous marker based on samples from a healthy population through preliminary experiments; The endogenous marker quantitative analysis is performed on the ionized input data by the carbon dioxide isotope internal standard quantitative method to obtain the content value of the predetermined endogenous marker type in the target human exhaled gas; Determine whether the content value of any type of endogenous marker obtained by quantitative analysis is within its corresponding normal value range. If so, mark it as normal; if not and above the normal value range, mark it as abnormally high; if not and below the normal value range, mark it as abnormally low.

[0010] Further, specifically, the endogenous marker quantitative analysis is performed on the ionized input data by the carbon dioxide isotope internal standard quantitative method to obtain the content value of the predetermined endogenous marker type in the target human exhaled gas, including: The carbon dioxide isotope peak used for quantification of endogenous markers is determined by preliminary experiments; By comparing the input data after ionization with the abundance of each endogenous marker, the isotope peak with a peak intensity close to that of the endogenous marker in exhaled breath was selected as the reference standard, and the relative ratios of different markers to the carbon dioxide isotope peak were analyzed for quantification.

[0011] Further, specifically, visually displaying the prediction results includes: The name of each endogenous marker, the corresponding content value and the abnormal analysis situation are associated and displayed visually in the form of a chart.

[0012] Furthermore, the method further comprises: The GC-MS device uses known endogenous volatile organic compounds commonly found in human exhaled breath as reference substances to perform high-resolution testing and analysis, and adjusts instrument parameters, including sensitivity, through preliminary experiments.

[0013] The beneficial effects of the present invention are: The present invention proposes a method for predicting human exhaled gas, in which the obtained target human exhaled gas is passed through a flow-through medium channel discharge source and a GC-MS device in series to perform result prediction based on a carbon dioxide isotope internal standard quantitative method. The whole process is highly real-time and easy to operate, and the components of human exhaled gas can be predicted quickly and conveniently. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The above and other features of the present disclosure will become more apparent by describing in detail the embodiments shown in the accompanying drawings. The same reference numerals in the accompanying drawings of the present disclosure represent the same or similar elements. Obviously, the accompanying drawings described below are only some embodiments of the present disclosure. For those skilled in the art, other accompanying drawings can be obtained based on these accompanying drawings without creative work. In the accompanying drawings: Figure 1 Shown is a flow chart of a method for predicting human exhaled gas according to the present invention. DETAILED DESCRIPTION

[0015] The following will be combined with the embodiments and drawings to clearly and completely describe the concept, specific structure and technical effects of the present invention, so as to fully understand the purpose, scheme and effect of the present invention. It should be noted that the embodiments in this application and the features in the embodiments can be combined with each other without conflict. The same reference numerals used throughout the drawings indicate the same or similar parts.

[0016] Example 1, reference Figure 1 The present invention proposes a method for predicting human exhaled gas, comprising the following steps: Step 110, obtaining exhaled gas of a target human body; Step 120, inputting the target human body's exhaled gas into the flow-through medium channel discharge source to output soft ionized gas molecules; Step 130, inputting the soft ionized gaseous molecules into the GC-MS device for ionization, and analyzing the ionized input data by the carbon dioxide isotope internal standard quantitative method to obtain a prediction result; Step 140: output the prediction result and perform a visual display.

[0017] In this embodiment 1, the obtained target human exhaled gas is passed through a series of flow-through medium passage discharge sources and a GC-MS device to perform result prediction based on the carbon dioxide isotope internal standard quantification method. The whole process is highly real-time and easy to operate, and the composition of human exhaled gas can be predicted quickly and conveniently.

[0018] As a preferred embodiment of the present invention, specifically, the method of obtaining and acquiring the exhaled gas of the target human body includes oral exhalation and nasal exhalation, wherein: For oral exhaled sampling, you need to pinch your nose and blow air into a disposable straw only with your mouth. For nasal exhaled sampling, you need to hold a fixed device in your mouth, place the head of the disposable straw on the outside of your nostril, and exhale alternately through both nostrils.

[0019] In this preferred embodiment, the breath sample collection method is optimized based on two modes: oral exhalation mode and nasal exhalation mode, and two breath collection devices are built, namely oral exhalation and nasal exhalation. For oral exhalation sampling, the nose needs to be pinched, and air is only blown into a disposable straw with the mouth. For nasal exhalation sampling, a fixed device needs to be placed in the mouth, the disposable straw head is placed outside the nostril, and both nostrils are exhaled alternately. Compare the differences between oral exhalation and nasal exhalation, and adopt the breath collection setting with high detection sensitivity, small external air interference, and good data repeatability under the current environment as needed.

[0020] As a preferred embodiment of the present invention, specifically, the acquired target human exhaled gas is directly set at the input end of the flow-through medium passage discharge source, and the output end of the flow-through medium passage discharge source is directly connected to the input end of the GC-MS device, that is, the flow-through medium passage discharge source and the GC-MS device are integrated.

[0021] In this preferred embodiment, the flow-through medium channel discharge source and the GC-MS device are "connected in series" in the above manner, and the components of the exhaled gas can be quickly predicted by simply collecting the exhaled gas from the human body, which is very convenient and efficient.

[0022] As a preferred embodiment of the present invention, the method further comprises: When obtaining the exhaled gas of the target human body, the exhaled gas of the target human body will also be pre-processed, that is, a disposable filter head is installed at the front end of the disposable straw, and the disposable filter head is used to filter out suspended particles and water vapor in the exhaled gas of the human body.

[0023] In this preferred embodiment, a disposable filter is installed at the front end of the straw to filter out suspended particles and most of the water vapor in the exhaled breath (it is known from previous preliminary experimental experience that the water vapor in the exhaled breath will condense and block the sample inlet) to avoid affecting the operating stability of the instrument and the test results.

[0024] As a preferred embodiment of the present invention, specifically, the ionized input data is analyzed by carbon dioxide isotope internal standard quantitative method to obtain prediction results, including: Determine the types of endogenous markers and the normal value range of each endogenous marker based on samples from a healthy population through preliminary experiments; Performing endogenous marker quantitative analysis on the ionized input data by using the carbon dioxide isotope internal standard quantitative method to obtain the content value of the pre-determined endogenous marker type in the target human exhaled gas; Determine whether the content value of any type of endogenous marker obtained by quantitative analysis is within its corresponding normal value range. If so, mark it as normal; if not and above the normal value range, mark it as abnormally high; if not and below the normal value range, mark it as abnormally low.

[0025] In this preferred embodiment, the 100 breath samples collected were first classified according to the research cohort, and the molecular ion peaks were identified and identified using the analysis software provided by the instrument, and quantitative analysis was performed using the standard gas external standard method. The content and relative proportion of common endogenous VOCs in the human body were studied, and a data array was established using control samples.

[0026] In order to organically combine breath analysis data with actual samples, multivariate statistical methods are an effective means of mathematical statistics, especially suitable for the analysis of complex high-throughput data, and are increasingly used in the study of breath characteristics. At present, the statistical methods involved include principal component analysis, cluster analysis, discriminant analysis, partial least squares analysis, etc., and the statistical analysis software involved includes SIMCA-P, SPSS, R, SAS, MATLAB, etc. This project intends to use SIMCA-P for principal component analysis and partial least squares analysis to find the corresponding characteristic substances of breath, and then use regression analysis and cluster analysis for data verification, and combine multiple statistical methods and statistical software for cross-validation to obtain more accurate and reliable analysis results.

[0027] Through data comparison and analysis, we finally screened out the characteristic endogenous VOCs in human exhaled breath and the relative content range of each compound, established an analysis model, and carried out experimental verification to determine the accuracy and sensitivity of the screening model. We established a dynamic, wide-range quantitative analysis method for exhaled VOCs based on "SOFT IONIZATION + DIRECT MASS".

[0028] As a preferred embodiment of the present invention, specifically, the endogenous marker quantitative analysis is performed on the ionized input data by the carbon dioxide isotope internal standard quantitative method to obtain the content value of the predetermined endogenous marker type in the target human exhaled gas, including: The carbon dioxide isotope peak used for quantification of endogenous markers is determined by preliminary experiments; By comparing the input data after ionization with the abundance of each endogenous marker, the isotope peak with a peak intensity close to that of the endogenous marker in exhaled breath was selected as the reference standard, and the relative ratios of different markers to the carbon dioxide isotope peak were analyzed for quantification.

[0029] As a preferred embodiment of the present invention, specifically, visually displaying the prediction results includes: The name of each endogenous marker, the corresponding content value and the abnormal analysis situation are associated and displayed visually in the form of a chart.

[0030] As a preferred embodiment of the present invention, the method further comprises: The GC-MS device uses known endogenous volatile organic compounds commonly found in human exhaled breath as reference substances to perform high-resolution testing and analysis, and adjusts instrument parameters, including sensitivity, through preliminary experiments.

[0031] Although the description of the present invention has been quite detailed and specifically describes several described embodiments, it is not intended to be limited to any of these details or embodiments or any particular embodiment, but should be regarded as providing a broad possible interpretation of these claims in view of the prior art by reference to the appended claims, thereby effectively covering the intended scope of the present invention. In addition, the above description of the present invention is based on the embodiments foreseeable by the inventor, and its purpose is to provide a useful description, and those non-substantial changes to the present invention that have not yet been foreseen may still represent equivalent changes to the present invention.

[0032] The above is only a preferred embodiment of the present invention. The present invention is not limited to the above implementation. As long as the technical effect of the present invention is achieved by the same means, it should belong to the protection scope of the present invention. Within the protection scope of the present invention, its technical scheme and / or implementation method can have various modifications and changes.

Claims

1. A method for predicting human exhaled gas, characterized in that: Includes the following: Acquire exhaled gas from a target human body; The target human exhaled gas is input into the flow-through medium channel discharge source to output soft ionized gas molecules; The soft ionized gaseous molecules are input into the GC-MS device for ionization, and the ionized input data are analyzed by the carbon dioxide isotope internal standard quantitative method to obtain the prediction results; The prediction results are output and visualized.

2. A method for predicting human exhaled gas according to claim 1, characterized in that: Specifically, the method of obtaining and acquiring the exhaled gas of the target human body includes oral exhalation and nasal exhalation, wherein: For oral exhaled sampling, you need to pinch your nose and blow air into a disposable straw only with your mouth. For nasal exhaled sampling, you need to hold a fixed device in your mouth, place the head of the disposable straw on the outside of your nostril, and exhale alternately through both nostrils.

3. The method for predicting human exhaled gas according to claim 1, characterized in that: Specifically, the acquired target human exhaled gas is directly set at the input end of the flow-through medium path discharge source, and the output end of the flow-through medium path discharge source is directly connected to the input end of the GC-MS device, that is, the flow-through medium path discharge source and the GC-MS device are integrated.

4. A method for predicting human exhaled gas according to claim 2, characterized in that: The method further comprises, When obtaining the exhaled gas of the target human body, the exhaled gas of the target human body will also be pre-processed, that is, a disposable filter head is installed at the front end of the disposable straw, and the disposable filter head is used to filter out suspended particles and water vapor in the exhaled gas of the human body.

5. The method for predicting human exhaled gas according to claim 1, characterized in that: Specifically, the ionized input data is analyzed by the carbon dioxide isotope internal standard quantitative method to obtain the prediction results, including: Determine the types of endogenous markers and the normal value range of each endogenous marker based on samples from a healthy population through preliminary experiments; Performing endogenous marker quantitative analysis on the ionized input data by using the carbon dioxide isotope internal standard quantitative method to obtain the content value of the pre-determined endogenous marker type in the target human exhaled gas; Determine whether the content value of any type of endogenous marker obtained by quantitative analysis is within its corresponding normal value range. If so, mark it as normal; if not and above the normal value range, mark it as abnormally high; if not and below the normal value range, mark it as abnormally low.

6. A method for predicting human exhaled gas according to claim 5, characterized in that: Specifically, the endogenous marker quantitative analysis is performed on the ionized input data by the carbon dioxide isotope internal standard quantitative method to obtain the content value of the predetermined endogenous marker type in the target human exhaled gas, including: The carbon dioxide isotope peak used for quantification of endogenous markers is determined by preliminary experiments; By comparing the input data after ionization with the abundance of each endogenous marker, the isotope peak with a peak intensity close to that of the endogenous marker in exhaled breath was selected as the reference standard, and the relative ratios of different markers to the carbon dioxide isotope peak were analyzed for quantification.

7. A method for predicting human exhaled gas according to claim 5, characterized in that: Specifically, visually displaying the prediction results includes: The name of each endogenous marker, the corresponding content value and the abnormal analysis situation are associated and displayed visually in the form of a chart.

8. The method for predicting human exhaled gas according to claim 1, characterized in that: The method further comprises, The GC-MS device uses known endogenous volatile organic compounds commonly found in human exhaled breath as reference substances to perform high-resolution testing and analysis, and adjusts instrument parameters, including sensitivity, through preliminary experiments.

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