Microfluidic metamaterial structure design method and microfluidic metamaterial structure

Abstract

The invention discloses a design method of a microfluidic metamaterial structure. The design method comprises the following steps: setting a microfluidic environment; calculating equivalent parameters of the micro-fluidic metamaterial; parametrically designing a micro-unit structure; constructing a regulation and control performance database; and constructing a micro-unit spatial topological structure. The invention also discloses a microfluidic metamaterial structure prepared based on the design method. The micro-fluid flow control effect of cooperatively regulating and controlling the flow speed and the flow direction of the micro-fluid in a specific area is achieved.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic technology, and in particular to a microfluidic metamaterial structure design method and a microfluidic metamaterial structure. Background Art

[0002] Microfluidic chips based on microfluidic technology have become one of the important technical means in biomedical engineering due to their advantages of high precision, low cost, fast analysis speed and modular functional integration. With the introduction of the concepts of precision medicine and precision medicine, microfluidic technology has provided important technical means for the rapid cultivation and detection of microorganisms such as cells and bacteria, the construction of organ chip models and biological scaffolds, the targeted delivery of anticancer drugs, the precise treatment of tumors, and the in vitro pathological reproduction of organs such as the uterus and intestines. In recent years, with the rapid development of aerospace technology and space science and technology, microfluidic chip technology has not only undertaken the regular and rapid monitoring of various biochemical indicators in astronauts' body fluids under special environments, but also provided strong technical support for the exploration of life in outer space and the detection of human habitable environments.

[0003] Microfluidic chip is an emerging micro-total analysis system that integrates multiple functional modules such as reagent inlet, sample inlet, microvalves, mixing devices, reactors, discharge systems, sensors, etc., with extremely high production accuracy and reaction resolution. Microfluidic chip technology integrates complex analysis processes such as sample pretreatment, preparation, reaction, detection and diagnosis involved in biochemical reactions and biological experiments into microfluidic chips of millimeter to centimeter size by precisely controlling trace fluids, thereby maximizing the elimination of artificial interference and quickly realizing the automated amplification and diagnosis of nucleic acid and other substances. The microscale channel structure of the microfluidic chip increases the reaction specific surface area on the chip, has a very high mass transfer efficiency, and also improves the high-throughput screening performance of the microfluidic chip, achieving faster and more sensitive detection.

[0004] However, due to the characteristics of low Reynolds number and laminar flow, achieving high-precision microfluidic control in microscale flow still faces huge challenges. Existing active microfluidic control technology can achieve precise control of microfluidics by applying external force to low Reynolds number fluids, but active microfluidic devices can damage biological samples due to high-frequency mixing and high-energy radiation. In addition, the design and production process of active microfluidic devices are complex, the operation is difficult, and the professional requirements are high. At the same time, the influence of the applied physical field on the physicochemical properties of microfluidic reagents must also be considered. The passive method does not require additional energy and does not involve any complex active energy excitation mechanism. Instead, it uses the flow of fluid through certain structural obstacles to improve the precision control efficiency of microfluidics. However, the existing passive microfluidic structures have complex designs, extremely high processing precision requirements, high processing difficulty, and are prone to microstructure blockage and difficult cleaning. The reusability is extremely poor, and it is difficult to obtain microfluidic devices with optimal performance, and it is difficult to meet the needs of modern biomedicine and other fields for multifunctional integrated microfluidic devices.

[0005] In addition, since biofilms and biological tissues are very sensitive to fluid flow rate and direction, cells or biological tissue cultures in co-culture environments must be independent of each other and do not interfere with each other. Currently, biomedicine uses a method of setting up multiple channels in parallel on a microfluidic chip to achieve co-culture of bacteria, cells and organ tissues. For multi-inlet parallel channel devices, more microvalve structures must be used to strictly control the flow rate, flow direction and input of nutrients in different channels to eliminate the influence of irrelevant factors, which requires higher skills and experience of microfluidic device operators.

[0006] Currently, the design of microfluidic devices is mostly based on experience and intuition, or relies on repeated attempts by microfluidic researchers to continuously improve the performance of the device. There is a lack of systematic understanding of the underlying mechanism of flow control of the micro-unit structure (microstructure shape, characteristic scale, microstructure layout angle, spatial topological distribution) in microfluidic devices.

[0007] Therefore, technicians in this field are committed to providing a microfluidic metamaterial chip structure design method and the resulting microfluidic metamaterial structure, to achieve microfluidic metamaterial topological structure design with coordinated regulation of microfluid flow rate and flow direction space. Summary of the invention

[0008] In view of the defects in the prior art, the technical problem to be solved by the present invention is how to provide a microfluidic metamaterial structure design method and a microfluidic material structure that can achieve coordinated regulation of microfluid flow rate and flow direction space.

[0009] To achieve the above object, the present invention provides a method for designing a microfluidic metamaterial structure, comprising the following steps:

[0010] Step 1: Setting up the microfluidic environment;

[0011] Step 2, calculating the equivalent parameters of the microfluidic metamaterial;

[0012] Step 3: parameterize and design the micro-unit structure;

[0013] Step 4: Build a control performance database;

[0014] Step 5: Construct the micro-unit spatial topological structure.

[0015] Furthermore, step 1 specifically includes:

[0016] Step 1.1, predefine the key parameters of microfluid flow rate and flow path in the microfluidic culture environment;

[0017] Step 1.2: Establish a spatial transformation theoretical model based on the key parameters.

[0018] Furthermore, the space transformation theoretical model includes: a space distortion transformation model, a space expansion transformation model, and a space compression-stretching transformation model.

[0019] Furthermore, in step 2, according to the spatial transformation theoretical model of step 1, an equivalent mapping between spatial transformation and microfluidic metamaterial is completed to obtain model parameters of microfluidic metamaterial.

[0020] Furthermore, in step 3, the characteristic size and rotational arrangement angle of the micro-unit structure are determined through finite element simulation and parametric scanning.

[0021] Furthermore, in step 4, numerical analysis and statistical methods are used to establish a microstructure flow field control performance database.

[0022] Furthermore, in step 5, a micro-unit spatial topological structure of the microfluidic metamaterial is constructed according to the control performance requirements.

[0023] Preferably, the method further comprises: verifying the precise control performance of the flow rate and flow direction of the topological structure.

[0024] Preferably, the method further comprises: performing multi-scale coupling control analysis to optimize the overall fluid control performance of the microfluidic metamaterial.

[0025] The present invention also provides a microfluidic metamaterial structure, which is designed by the above-mentioned design method.

[0026] The present invention has at least the following beneficial technical effects:

[0027] The microfluidic metamaterial structure design method of the present invention constructs a design method that unifies the multi-scale collaborative coupling of "microstructure shape-characteristic scale-rotation angle-spatial topological structure" of microfluidic metamaterials and microfluidic performance, thereby achieving a microfluidic flow control effect of collaboratively regulating the microfluidic flow velocity and flow direction in a specific area.

[0028] The microfluidic metamaterial structure of the present invention adjusts the flow rate and direction of the microfluid in a specific local area, provides a micro-culture environment in which each culture area is independent and does not interfere with each other, and meets the co-culture requirements in biomedical engineering.

[0029] The concept, specific structure and technical effects of the present invention will be further described below in conjunction with the accompanying drawings to fully understand the purpose, characteristics and effects of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 is a schematic diagram of a flow chart of a method for designing a microfluidic metamaterial structure according to an embodiment of the present invention;

[0031] Figure 2 is a schematic diagram of a space transformation theoretical model of an embodiment of the present invention;

[0032] Figure 3 Schematic diagram of the multifunctional coupling design with adjustable flow pattern according to an embodiment of the present invention;

[0033] Figure 4 is a schematic diagram of the spatial topological design of the microfluidic metamaterial structure according to an embodiment of the present invention;

[0034] Figure 5 is a schematic diagram of a microfluidic metamaterial rotational mixing structure according to an embodiment of the present invention;

[0035] Figure 6 Schematic diagram of precise control of flow rate of metamaterial micro-flow according to an embodiment of the present invention;

[0036] Figure 7 It is a schematic diagram of a coupling model for precise control of microfluid flow rate and flow direction according to an embodiment of the present invention. DETAILED DESCRIPTION

[0037] The following describes the preferred embodiments of the present invention to make its technical content clearer and easier to understand. The present invention can be embodied in many different forms of embodiments, and the protection scope of the present invention is not limited to the embodiments mentioned in the text.

[0038] In the drawings, components with the same structure are indicated by the same numerical reference numerals, and components with similar structures or functions are indicated by similar numerical reference numerals. The size and thickness of each component shown in the drawings are arbitrarily shown, and the present invention does not limit the size and thickness of each component. In order to make the illustration clearer, the thickness of the components is appropriately exaggerated in some places in the drawings.

[0039] The present invention provides a method for designing a microfluidic metamaterial structure. Starting from the basic control equations of fluid dynamics, a design method for unifying the multi-scale collaborative coupling and microfluidic performance of "microstructure shape-characteristic scale-rotation angle-spatial topological structure" of microfluidic metamaterials is constructed to achieve a microfluidic flow control effect of collaboratively regulating the microfluidic flow velocity and flow direction in a specific area.

[0040] like Figure 1 As shown, the implementation process of a specific embodiment of the microfluidic metamaterial structure design method of the present invention is as follows.

[0041] Step 1: Set up the microfluidic environment. This step specifically includes the following processes:

[0042] Step 1.1, predefine the key parameters of the microfluid flow rate and flow path in the microfluidic culture environment, wherein the key parameters include the microfluid flow rate, flow path, etc.

[0043] Step 1.2: Establish a space transformation theoretical model based on the above key parameters, wherein the space transformation theoretical model includes: space distortion transformation model, space expansion transformation model, and space compression-stretching transformation model.

[0044] Establishing a tunable microfluidic theoretical model of microfluidic metamaterials is the basis for precise regulation and control of the flow state of microfluids. As the main control equation for fluid flow, the Navier-Stokes equation must first be kept invariant in form when performing coordinate transformation operations; then the flow trajectory of the fluid is pre-set according to the needs of flow field regulation, and the coordinate transformation theoretical model between the original fluid space and the transformed space is established by transforming the original space by distortion, expansion, compression and stretching. Due to the laminar flow and low Reynolds number characteristics of microfluidics, how to establish a microfluidic control model that is not restricted by external pressure fields or velocity fields and can quickly produce tunable high flow rates and high rotation effects is the key to achieving efficient mixing of microfluids. The spatial transformation theoretical model of this embodiment is as follows:

[0045] like Figure 2As shown in Figure A, microfluidic rotation mixing and flow direction control are achieved through a spatial distortion transformation model. In this model, first, the rotation angle of the flow field in a specific target area is determined according to the mixing requirements of microfluidics. Specifically, assuming that the two-dimensional space is divided into three regions by shell-shaped metamaterials, according to the spatial transformation theory, to achieve the rotation mixing effect of the microfluidic field, the corresponding coordinate mapping transformation is as follows:

[0046] Region I: r < R1, r' = r, z' = z, θ' = θ + θ0;

[0047] Region II: r > R2, r' = r, z' = z, θ' = θ;

[0048] Region III: R1 < r < R2, r' = r, z' = z,

[0049] where,

[0050] This set of spatial mapping transformation relationships causes a θ0 angle difference in the flow fields of Region I and Region III. The rotation angle θ0 can be increased or decreased according to the mixing requirements. When r = R1 = R2, the flow field will not have a rotation mixing effect; when r = R1 = 0, the rotation mixing effect will occur in the entire flow field area where r < R2.

[0051] Then, based on the above mapping transformation, the transformation mapping relationship corresponding to the specific rotation area between the original coordinate space and the transformed space is established.

[0052] As Figure 2 shown in Figure B, microfluidic flow rate regulation and co-culture models are achieved through a spatial expansion transformation model. Specifically, assuming that the two-dimensional space is divided into three regions by shell-shaped metamaterials: Region I (r < R1) is the static regulation area of the microfluidic field; Region II (R1 < r < R2) is the functional area of the microfluidic metamaterials; Region III (r > R2) is the background flow field area outside the metamaterial structure, that is, the area where no spatial transformation operation is required. To achieve the effect of specific area isolation, the corresponding coordinate mapping transformation is as follows:

[0053]

[0054] In the formula,

[0055] At this time, at the center point 0 of the original coordinate space, through the transformation operation, it expands into an annular area with a radius of R1 in the transformed space. Using this area, the function of static isolation culture of the microfluidic field can be achieved. Using this model, a static culture area can be created, in which the fluid is almost static, so as to store the targeted drug for directional drug delivery.

[0056] Through the space expansion transformation model, a microfluidic metamaterial cloak region that isolates the mutual influence of the internal and external flow fields of a specific region is constructed, enabling multiple regions in the same culture microenvironment to be independent and non-interfering with each other. This plays a crucial role in implanting in-vivo micro-devices and ensuring that they do not interfere with or damage the original flow and functions in the body, as well as for monitoring and comparison during co-culture of cells, bacteria, and organ tissues.

[0057] As Figure 2 shown in Figure C of [reference], the microfluidic flow rate control and culture medium concentration regulation are achieved through the space compression-stretching transformation model. Specifically, assume that the two-dimensional space is divided into four regions by the shell-shaped metamaterial: Region I (r < R1) is the micro-flow state control region; Regions II (R1 < r < R2) and III (R2 < r < R3) are the microfluidic metamaterial regulation structure regions; Region IV (r > R3) is the background flow field region outside the metamaterial structure. To achieve dynamic regulation of the flow state, the corresponding coordinate transformation relationship is:

[0058]

[0059] The specific process is as follows: First, the space defined by the green dashed line R2 is uniformly compressed into the inner layer R1; second, the space between the green R2 and red R3 boundaries is non-uniformly stretched into the middle and outer layers, that is, stretched into Regions II and III (R1 < r < R3). Through this operation, the flow field with a radius of R2 in the original space is compressed into the region enclosed by the transformed space R1, and the flow field between R2 and R3 in the original space is stretched into the region between spaces R1 and R3. This theoretical model can dynamically adjust the flow velocity magnitude in the core flow field control Region I by adjusting the relative proportional relationship between the characteristic scales of R1, R2, and R3.

[0060] Through the space compression-stretching transformation model, a functional region that can dynamically adjust the flow state of the microfluidic field according to the control requirements of actual microfluids can be constructed. Since the growth of bacterial biofilms, cells, and biological tissues is very sensitive to changes in fluid flow, and there are also significant differences in the requirements for the flow environment at different stages of their cultivation; during the cultivation of microorganisms and biological tissues, the exchange of nutrients and the discharge of waste in the cultivation device can be achieved by adjusting the flow state of specific regions. When an increase in drug dosage is required, this theoretical model precisely controls the drug release rate by regulating the flow state of the drug storage region and can be dynamically adjusted according to the patient's treatment stage, providing a solution for achieving high-precision and efficient targeted drug delivery.

[0061] Step 2: Calculate the equivalent parameters of the microfluidic metamaterial. In this step, according to the space transformation theoretical model in Step 1, the space transformation and the equivalent mapping of the microfluidic metamaterial are completed to obtain the model parameters of the microfluidic metamaterial.

[0062] Specifically, coupling the spatial compression-stretching transformation model with the spatial expansion transformation model can collaboratively achieve targeted drug delivery and sustained sustained-release therapy to the lesion; coupling the spatial compression-stretching transformation model with the spatial distortion transformation model can collaboratively achieve coordinated regulation of flow velocity and flow direction in the core process control area. The coupling process is as follows: Figure 3 shown.

[0063] Step 3: Parametric design of micro-unit structure In this step, the characteristic size and rotational arrangement angle of the micro-unit structure are determined through finite element simulation and parametric scanning.

[0064] After obtaining the theoretical model of tunable microflow control of microfluidic metamaterials, in the actual physical space, according to the distribution of transformation tensors in the target geometric area, the microfluidic metamaterial technology is used to design the anisotropic microscale equivalent medium required for spatial transformation, completing the equivalence of metamaterial and spatial transformation operations, thereby realizing the required flow regulation function and enhancing the control over the fluid motion process.

[0065] After the spatial transformation, the constant material (fluid density, dynamic viscosity) in the original Navier-Stokes equation is transformed into a tensor form. Ignoring the body force source term, the conservation form of the original control equation Navier-Stokes is simplified to:

[0066]

[0067] By adopting the space transformation processing, the Navier-Stokes equation in the transformation space is expressed as follows:

[0068]

[0069] Among them, the density in the transformation space and the dynamic viscosity coefficient is transformed into the form of an inhomogeneous anisotropic tensor. ρ and μ in the original space and in the new transformed space and The corresponding relationship between them is:

[0070]

[0071] The expression obtained by agreement is: and in,

[0072] In three-dimensional space, the transformation tensor It has multiple off-diagonal elements and exhibits spatial anisotropy. With the help of the basic properties of matrix theory, it is clear that the transformation tensor is a real symmetric matrix. The transformation tensor after matrix diagonalization has 3 remaining diagonal elements. The diagonalized matrix consists of 5 diagonal elements. Reduced to 3 The complexity of metamaterial structure design is reduced. In order to solve the continuous change of the properties of the transformation tensor space, the fluid metamaterial is constructed by piecewise integral approximation and hierarchical design using numerical analysis and discrete methods.

[0073] like Figure 4 As shown in the figure, the diagonalized matrix and the original transformation tensor matrix introduce a spatial rotation angle α in the coordinate principal axis. Therefore, after the real symmetric transformation tensor matrix is ​​processed, it is necessary to confirm the rotation correspondence α between the spatial coordinate principal axes before and after the matrix diagonalization, that is, the rotation angle α of the coordinate axis needs to be incorporated into the design process of the metamaterial micro-unit structure.

[0074] In addition to the micro-unit structure, this step also characterizes the rectangular micro-column units required for the microfluidic functional area, and the micro-columns to maintain uniform flow in the flow field outside the functional area. The purpose of the micro-columns is to match the surrounding flow field, thereby maintaining the microfluidic areas without affecting each other. The characteristic scale of the micro-rectangular unit and micro-column structure is based on It is obtained by finite element numerical simulation.

[0075] The microfluidic field disturbance characteristics induced by the shape, characteristic scale and rotation angle of the metamaterial micro-unit are characterized by combining finite element numerical simulation and the concept of equivalent hydrodynamic performance in porous media theory. When constructing microfluidic metamaterials, a corresponding number of microstructured crystal cells (the concept of spatial discreteness) are divided in each layer of the metamaterial structure, and each crystal cell is filled with a certain form of micro-column structure at a specific rotation angle to manufacture the metamaterial structure required for microfluidic control. The effective fluid dynamics performance of the micro-unit structure of the metamaterial is jointly determined by factors such as the scale of the micro-unit cell, the shape, characteristic scale and rotation angle of the micro-unit column in the unit cell, and has nothing to do with the material properties of the micro-unit composite material itself.

[0076] Step 4: Constructing a control performance database. In this step, numerical analysis and statistical methods are used to establish a microstructure flow field control performance database.

[0077] Specifically, according to the requirements of microfluidic precise control, the micro-unit structures of the parametric scan in step 3 are grouped. For example, if the microfluidic flow direction control requires 15°, 30°, 60°, and 90°, the size and spatial rotation angle of the micro-unit correspond to them respectively.

[0078] The microfluid flow direction of a specific embodiment of the present invention is regulated by a micro-unit structure characteristic size (x l ,yl ), rotation angle (α), and the diameter (d_c) of the uniform micro-cylinder array to maintain the flow fields between functional areas without affecting each other are shown in Table 1.

[0079] Table 1

[0080]

[0081] Step 5: Constructing a micro-unit spatial topological structure. In this step, the micro-unit spatial topological structure of the microfluidic metamaterial is constructed according to the control performance requirements.

[0082] like Figure 5 As shown in the figure, due to the differences in the types of microfluidic chips when culturing different microorganisms or biomacromolecules, the requirements for the degree and intensity of microfluidic mixing are significantly different. Therefore, according to the flow rate and flow direction control performance requirements, micro-unit structures with specific shapes, characteristic scales and rotation angles are selected. For example, micro-unit structures can be placed in layers in the form of concentric rings at equal intervals to obtain the micro-unit spatial topological structure.

[0083] Some embodiments of the present invention further include the following steps.

[0084] Step 6: Verify the topological structure's precise control performance of flow rate and flow direction.

[0085] In order to effectively solve the sensitivity of microbial culture, organ chip construction, and life material amplification in micro-culture environments in biomedical engineering to flow velocity and direction, and to reduce the damage caused by implantable micro-devices to the original flow and function in the body, it is necessary to construct a functionally tunable micro-flow precision control metamaterial structure. For a static culture environment, based on the microstructure characterization results in step 3, a static culture metamaterial structure that isolates a specific area from the external flow field or the interaction between regions, i.e., a cylindrical array, can be constructed. The results are shown in Figure 2. Figure 6 shown.

[0086] Some other embodiments of the present invention further include the following steps.

[0087] Step 7: Conduct multi-scale coupling control analysis to optimize the overall fluid control performance of the microfluidic metamaterial.

[0088] For a dynamically tunable microfluidic culture environment, it is necessary to construct a specific area that can dynamically adjust the microfluidic flow velocity according to the actual flow requirements based on the established compression-stretch transformation theoretical model between the original space and the transformed space. In order to effectively solve the problem of the sensitivity of living matter to the flow direction, by coupling the spatial coordinate compression-stretch transformation model and the rotation transformation theoretical model, and then based on the above characterization results, a microfluidic metamaterial structure model with dynamically tunable microfluidic flow velocity and flow direction is finally obtained. The results are shown in Figure 7As shown, the innermost layer is a micro-rectangular array with a microfluidic rotation effect and a constant angle with the radial direction; outside the innermost layer is a layered array of micro-rectangular columns (with no angle) for regulating the fluid flow rate, with the main axis of the rectangular microcolumns parallel to the radial direction; the functional area is surrounded by a micro-cylinder array.

[0089] The present application proposes a design method for a microfluidic metamaterial structure, and the microfluidic metamaterial structure obtained by the design method is also within the protection scope of the present application.

[0090] The present invention presets the fluid flow trajectory according to the flow field regulation requirements, and establishes a theoretical model of coordinate transformation between the original fluid space and the transformed space by rotating, expanding, compressing and stretching the original space. At the same time, a compression-stretching transformation relationship model is constructed to achieve dynamic adjustment of the flow state of the microfluidic field, and coordinately complete the regulation of the flow velocity and flow direction in the core flow field control area. The spatial variation theoretical models of the present invention are all limited to a specific or multiple functional areas, so they only perform regulation of the microfluid flow velocity and flow direction in a specific local area. The culture areas will not interfere with each other, nor will they affect the culture environment outside the functional area. All established control mechanisms and theoretical models can meet the co-cultivation requirements in biomedical engineering.

[0091] The preferred specific embodiments of the present invention are described in detail above. It should be understood that a person skilled in the art can make many modifications and changes based on the concept of the present invention without creative work. Therefore, any technical solution that can be obtained by a person skilled in the art through logical analysis, reasoning or limited experiments based on the concept of the present invention on the basis of the prior art should be within the scope of protection determined by the claims.

Claims

1. A method for designing a microfluidic metamaterial structure, characterized in that: The following steps are involved: Step 1: Setting up the microfluidic environment; Step 2, calculating the equivalent parameters of the microfluidic metamaterial; Step 3: parameterize and design the micro-unit structure; Step 4: Build a control performance database; Step 5: Construct the micro-unit spatial topological structure.

2. The microfluidic metamaterial structure design method according to claim 1, characterized in that: Step 1 specifically includes: Step 1.1, predefine the key parameters of microfluidic flow rate and flow path in the microfluidic culture environment; Step 1.2: Establish a spatial mapping transformation theoretical model based on the key parameters.

3. The microfluidic metamaterial structure design method according to claim 2, characterized in that: The space transformation theoretical model includes: a space distortion transformation model, a space expansion transformation model, a space compression-stretching transformation model and a coupling model thereof.

4. The microfluidic metamaterial structure design method according to claim 2, characterized in that: In step 2, according to the theoretical model of spatial transformation described in step 1, the equivalent mapping between spatial transformation and microfluidic metamaterial is completed to obtain the model parameters of the microfluidic metamaterial.

5. The microfluidic metamaterial structure design method according to claim 4, characterized in that: In step 3, the characteristic size and rotational arrangement angle of the micro-unit structure are determined through finite element simulation and parametric scanning.

6. The microfluidic metamaterial structure design method according to claim 5, characterized in that: In step 4, numerical analysis and statistical methods are used to establish a microstructure flow field control performance database.

7. The microfluidic metamaterial structure design method according to claim 6, characterized in that: In step 5, the micro-unit spatial topological structure of the microfluidic metamaterial is constructed according to the control performance requirements.

8. The microfluidic metamaterial structure design method according to claim 1, characterized in that: Also includes: The topology is verified for its ability to precisely regulate flow rate and direction.

9. The microfluidic metamaterial structure design method according to claim 1, characterized in that: Also includes: Conduct multi-scale coupling control analysis to optimize the overall fluid control performance of microfluidic metamaterials.

10. A microfluidic metamaterial structure, characterized in that: The method is designed using the design method described in any one of claims 1 to 9.

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