Five-ingredient mannan butter ointment for treating skin diseases as well as preparation method and application of five-ingredient mannan butter ointment

Abstract

The invention provides a five-ingredient mannan butter ointment for treating skin diseases and a preparation method and application thereof.The five-ingredient mannan butter ointment is prepared through the following steps that herba ephedrae, herba artemisiae annuae, rhododendron simsii, platycladus orientalis and myricaria laxiflora which are equal in weight are taken and put into a swing type smashing machine to be smashed, medicinal materials are smashed to a 40-mesh sieve, and the smashing time ranges from 5 min to 10 min; distilled water is added into the medicinal material powder for heating, boiling and extracting, the material-liquid ratio is 1: 10, extraction is conducted twice, extraction lasts for 30-35 min each time, extracting solutions are combined, filtered and concentrated, and a Wuwei manna water extract is obtained; the preparation method comprises the following steps: preparing the following raw materials in parts by weight: 100 parts of butter, 100 parts of polyethylene glycol, 50 parts of gelatin, 50 parts of stearic acid, 50 parts of span and 200 parts of a Wuwei mannan aqueous extract; and stirring to form an emulsion with uniform color. The ointment can effectively treat eczema, adopts traditional Chinese medicinal materials and butter as raw materials, is safe and effective, has low toxic and side effects, is mild and non-irritant to skin, and is expected to provide a brand new ointment for treating skin diseases.

Description

[Technical field]

[0001] The invention relates to the technical field of medicines, and in particular to a five-flavor nectar butter ointment for treating skin diseases, and a preparation method and application thereof. [Background technology]

[0002] Wuwei Ganlu is composed of Rhizoma Atractylodis Macrocephalae, Juniperus chinensis leaves, Juniperus chinensis branches, Ephedra sinica, and Semen scabra. The single medicine is generally ground into powder in a ratio of 1:1:2:2:3 for standby use. Wuwei Ganlu has the functions of regulating body elements, removing dry yellow water, relaxing muscles and dispersing knots, invigorating qi and tonifying the kidney, dredging meridians and relieving pain. It is often used for skin diseases, rheumatic diseases, obsessive-compulsive arthritis and other diseases. Generally, yellow water skin diseases are treated by rubbing bath or medicated bath. The ointment prepared by Wuwei Ganlu for external use in the treatment of skin diseases such as eczema has the advantages of convenience, economy, and high patient compliance, and is worthy of research and development and wide promotion. The preparation process of Wuwei Ganlu ointment has been authorized by China Patent. The Chinese invention patent with patent number CN202110996300.X discloses a Wuwei Ganlu ointment with anti-inflammatory effect and its preparation method, and specifically records the preparation process of Wuwei Ganlu ointment.

[0003] Butter has high nutritional value, and its main components are fat and water. Among the fatty acids contained in butter, oleic acid has the highest content. Human plasma, cell membranes, etc. all contain oleic acid. Oleic acid affects the integrity of cell membranes and is an essential fatty acid for the human body. In addition to oleic acid, butter also contains linolenic acid, linoleic acid, etc. Linolenic acid and linoleic acid are essential fatty acids for the human body. Linoleic acid is an essential substance for the synthesis of cephalin, and has a certain preventive effect on infant eczematous dermatitis. In addition, Mongolian butter also contains VA, VD, VE, VB1, VB2, carotenoids, phospholipids, minerals, etc. Vitamin A deficiency can lead to dry skin, followed by excessive keratinization and reduced glandular secretion, leading to dry skin, easy desquamation, itching, etc. Studies have found that insufficient vitamin D in the blood is a risk factor for increasing diseases such as neonatal eczema and infantile atopic dermatitis. In addition, topical vitamin E can improve the barrier function of the skin of children, reduce the entry of allergens and irritants into the body, reduce the sensitivity of the skin to various external stimuli, and relieve dryness, desquamation and the itching caused by it. Therefore, it can be concluded that the fatty acids and multivitamins contained in Mongolian butter are beneficial to the treatment of infant eczema. Butter is a sticky substance, easy to apply, easy to prepare ointment, has no adverse reactions to external use, and is highly safe. The fatty acids it contains have the effect of moisturizing the skin and preventing eczema, and the nutrients it contains can be used to treat infant eczema. Therefore, in this study, butter is used as a carrier, and different concentrations of Mongolian medicine Wuwei Ganlu are fully mixed and dissolved in the butter, and a professional ointment preparation process is used. [Summary of the invention]

[0004] One of the technical problems to be solved by the present invention is to provide an ointment for treating skin diseases - Wuwei Ganlu Butter Ointment, which can effectively treat eczema. It uses butter as a carrier, and the Wuwei Ganlu Butter Ointment made by fully mixing and dissolving Wuwei Ganlu in the butter can achieve twice the result with half the effort in treating eczema. It is safe and effective, has low toxic and side effects, is mild and non-irritating to the skin, and is expected to provide a new ointment for treating skin diseases.

[0005] The present invention achieves one of the above-mentioned technical problems by:

[0006] A five-flavor nectar oil ointment for treating skin diseases, wherein the raw materials and weight portions of the five-flavor nectar oil ointment are as follows:

[0007] 100 parts of butter, 100 parts of polyethylene glycol, 50 parts of gelatin, 50 parts of stearic acid, 50 parts of Span and 200 parts of five-flavor nectar aqueous extract;

[0008] The raw materials of the five-flavor nectar water extract are equal weights of ephedra, spatholobus dasyphylla, spatholobus chinensis, arborvitae and cypress branches.

[0009] Furthermore, the preparation method of the five-flavor nectar water extract is as follows:

[0010] Put 200g of Ephedra, 200g of Pinus dasyphylla, 200g of Rhizoma Cibotii, 200g of Platycladus orientalis and 200g of Cypress branches into a rocking type crusher and grind the medicinal materials through a 40-mesh sieve to obtain medicinal powder; add distilled water to the medicinal powder and heat and boil to extract, with a solid-liquid ratio of 1:10, extract twice, each time for 30-35 minutes, combine the extracts, filter, and concentrate to 1L.

[0011] The second technical problem to be solved by the present invention is to provide a method for preparing the five-flavor nectar butter ointment for treating skin diseases, which has a simple and rapid process, and a gentle and easy-to-operate preparation process.

[0012] The present invention achieves the second of the above technical problems in this way:

[0013] A method for preparing five-flavor nectar butter ointment for treating skin diseases, the preparation method comprising the following steps:

[0014] Step 1: Crush the medicinal materials:

[0015] Take equal weights of ephedra, scutellaria baicalensis, scutellaria baicalensis, arborvitae and cypress branches, put them into a swing-type crusher and grind them into powder. Grind the medicinal materials to a 40-mesh sieve for 5-10 minutes.

[0016] Step 2: Extraction:

[0017] Add distilled water to the powdered medicinal material for heating and boiling extraction, with a solid-liquid ratio of 1:10, and extract twice, each time for 30-35 minutes, combine the extracts, filter, and concentrate to obtain the five-flavor nectar water extract;

[0018] Step 3: Preparation of ointment

[0019] The following raw materials in parts by weight: 100 parts of butter, 100 parts of polyethylene glycol, 50 parts of gelatin, 50 parts of stearic acid, 50 parts of Span and 200 parts of Schisandra nectar aqueous extract; stir until a milky state with uniform color is formed.

[0020] Furthermore, the raw material dosages of the Wuwei Ganlu water extract are as follows: 200g of Ephedra, 200g of Pinus dasyphylla, 200g of Rhizoma Cibotii, 200g of Platycladus orientalis and 200g of Cypress Branch; after concentration, the obtained Wuwei Ganlu water extract is 1L.

[0021] Furthermore, the five-flavor nectar butter ointment for treating skin diseases is used in the preparation of a medicine for treating skin eczema.

[0022] The present invention has the following advantages:

[0023] The five-flavor nectar butter ointment of the present invention adopts Chinese medicinal materials and butter as raw materials, is characterized in that butter is used as a carrier, and butter is a sticky object, easy to apply, easy to prepare an ointment, has no adverse reactions for external use, is highly safe, and the fatty acids contained therein have the effects of moisturizing the skin and preventing eczema. The five-flavor nectar butter ointment is more effective in treating eczema than the simple five-flavor nectar treatment. In short, the five-flavor nectar butter ointment of the present invention is safe and effective, has low toxic and side effects, is mild and non-irritating to the skin, can be used to treat infantile eczema, and is expected to provide a new ointment for treating skin diseases.

Brief Description of the Drawings

[0024] The present invention will be further described below in conjunction with embodiments with reference to the accompanying drawings.

[0025] Figure 1 Schematic diagram of skin pathological sections of rats in various groups in the embodiments of the present invention; A: model group; B: blank control group; C: Wuwei Ganlu group; D: positive control drug group; E: Wuwei Ganlu Huangyou ointment group.

[0026] Figure 2 3 is a comparative schematic diagram of the contents of TNF-α, IL-1β, IL-2, IL-4 and IFN-γ in the serum of rats in each group in the examples of the present invention. [Specific implementation method]

[0027] The technical scheme of the present invention will be clearly and completely described below in conjunction with specific embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work are within the scope of protection of the present invention. If specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0028] Example

[0029] 1. Preparation of Wuwei Ganlu Butter Ointment

[0030] 1. Medicinal materials

[0031] Medicinal materials: Ephedra (batch number: 210611), Artemisia annua (batch number: 220341), Rhizoma Zhaobai (batch number: 210903), Platycladus orientalis (batch number: 200624), and Cypress twig (221106).

[0032] 2. Main instruments and equipment

[0033] Ultrasonic cleaner (Changzhou Ronghua, HDM-250D), electronic balance (Changshu Jiaheng DT-500B), rotary evaporation system (Shanghai Airang, N-1300), circulating water vacuum pump (Gongyi Yingyu Yuhua, SHZDⅢ), cooling water circulation device (Shanghai Airang, CCA-1112A), sealed swing crusher (Chuangli Medicinal Equipment, CLF-10C).

[0034] 3. Preparation method

[0035] (I) Medicinal material crushing

[0036] Take the raw materials of ephedra (200g), scutellaria baicalensis (200g), scutellaria baicalensis (200g), arborvitae (200g), and water cypress branches (200g) and put them into a swing-type crusher for powdering. The medicinal materials are crushed to a 40-mesh sieve for 5-10 minutes.

[0037] (II) Extraction

[0038] Add distilled water (1:10) to the powdered medicinal material in a round-bottom flask and heat and boil for extraction. Extract twice, each time for 30 minutes. Combine the extracts, filter, and concentrate to 1L.

[0039] (III) Ointment preparation

[0040] 100g butter, 100g polyethylene glycol, 50g gelatin, 50g stearic acid, 50g Span, 200ml medicinal material extract, stir until it forms an emulsion with uniform color.

[0041] 2. Study on the animal model of eczema treated with Wuwei Ganlu butter ointment

[0042] In order to clarify the effect of Wuwei Ganlu Huangyou Ointment in treating eczema, a rat eczema model was established, and the efficacy of Wuwei Ganlu Huangyou Ointment in treating eczema was tested by testing the relevant verification indicators in rat serum. The specific steps and results are as follows:

[0043] 1. Research subjects: 30 4-week-old SPF healthy SD rats, half male and half female, with a body weight of 200-220 g, certificate number: 110324210106503772, provided by Suzhou Xishan Biotechnology Co., Ltd., production license number: SCXK (Beijing) 2019-0010, fed under standard conditions.

[0044] Main instruments: Multifunctional microplate reader (Enspire, PerkinElmer, Germany); Centrifuge (Centrifuge5430 and 5180R, Eppendorf, Germany).

[0045] Rat TNF-α (EM0183), IL-1β (EM0109), IL-2 (EM0112), IL-4 (EM0119), and IFN-γ (EM0093) ELISA kits were purchased from Wuhan Fine Biotechnology Co., Ltd.

[0046] 2. Establish eczema model:

[0047] A blank control group, a model group, a positive control drug group, a Wuwei Ganlu group, and a Wuwei Ganlu Huangyou ointment group were established, and a rat eczema model was established: the blank control group was not treated specially. The rats in the model group, the positive control drug group, the Wuwei Ganlu group, and the Wuwei Ganlu Huangyou ointment group were all established with an eczema model, and the specific method was as follows: the rats were depilated on the back, 100 μL of 7% DNCB solution was applied to the skin for sensitization, and 25ul of 0.5% DNCB solution was applied on the 6th, 9th, 12th, and 15th days for continuous modeling, and finally a rat eczema model was established.

[0048] 3. Treatment method: Divide into 5 groups, with 6 rats in each group. Start treatment 24 hours after successful stimulation of model building, apply corresponding drugs and reagents every day, and continue treatment for 15 days.

[0049] Blank control group: no treatment.

[0050] Model group: 0.9% saline solution was applied.

[0051] Positive control group: Apply paeonol ointment (a commonly used drug for clinical treatment of eczema).

[0052] Wuwei Ganlu group: applied Wuwei Ganlu concentrate (the Wuwei Ganlu concentrate extracted in step (ii) of the ointment preparation process).

[0053] Wuwei Ganlu Butter Ointment Group: The Wuwei Ganlu Butter Ointment prepared above was applied.

[0054] 4. Observation indicators:

[0055] 4.1HE staining was used for pathological sectioning to observe the pathological changes of skin tissue in each group.

[0056] 4.2 Test the levels of TNF-α, IL-1β, IL-2, IL-4, and IFN-γ in rat serum.

[0057] 4.3 GraphPadPrism 9 statistical software was used for analysis. The measurement data were expressed as mean ± standard deviation. One-Way ANOVA was used for comparison among multiple groups. P < 0.05 was considered statistically significant.

[0058] 5. Experimental results:

[0059] 5.1 Pathological section results see Figure 1 ,from Figure 1 It can be seen from the results: Blank control group: The boundary between the epidermis and dermis is clear, the structure is clear, and there is no abnormal edema, vasodilation or inflammatory cell infiltration. Model group: The epidermis is imperfect, there are signs of damage, the epidermis is thickened, and the internuclear space in the superficial dermis is enlarged, indicating edema. Lymphocytes are chaotically arranged in the epidermis, indicating acute inflammation. Wuwei Ganlu group: The boundary between the epidermis and dermis is relatively clear, there is still a small amount of edema in the dermis, and a small amount of inflammatory cell infiltration can be seen. Positive drug control group: The epidermis has a clear structure, but edema can still be seen in the dermis, and the number of inflammatory cells is less than that in the model group. Wuwei Ganlu butter ointment group: The boundary between the epidermis and dermis is clear, the edema of epidermal cells is basically absorbed, and the inflammatory cells and tissue cells in the dermis are significantly reduced compared with the model group.

[0060] 5.2 Test results of TNF-α, IL-1β, IL-2, IL-4, and IFN-γ in rat serum are shown in Tables 1-5 and Figure 2 , it can be seen that: ① Compared with the blank group, the relevant inflammatory indexes in the model group were significantly increased; ② Compared with the model group, the relevant inflammatory indexes in the serum of rats in the paeonol ointment treatment group, schiwei ganlu and schiwei ganlu butter ointment groups were significantly decreased; ③ In the schiwei ganlu butter ointment group, the levels of inflammatory factors such as TNF-α, IL-1β, IL-2, IL-4 and IFN-γ in the serum of rats were significantly lower than those in the model group; ④ The levels of TNF-α, IL-1β, IL-4 and IFN-γ were lower than those in the treatment with simple schiwei ganlu concentrate, indicating that schiwei ganlu butter ointment has the effect of treating eczema, and the effect is better than that of schiwei ganlu alone.

[0061] Table 1 Changes in serum tumor necrosis factor α (TNF-α) levels in rats (pg / ml)

[0062]

[0063]

[0064] Note: a represents P<0.05 compared with the blank group; b represents P<0.05 compared with the model group; c represents P<0.05 compared with the positive control group; d represents P<0.05 compared with the Wuwei Ganlu group; the same as the following table;

[0065] Table 2 Changes in serum interleukin-1β (IL-1β) content in rats (pg / ml)

[0066]

[0067] Table 3 Changes in serum interleukin-2 (IL-2) content in rats (pg / ml)

[0068]

[0069] Table 4 Changes in serum interleukin-4 (IL-4) content in rats (pg / ml)

[0070]

[0071]

[0072] Table 5 Changes in serum interferon-γ (IFN-γ) content in rats (pg / ml)

[0073]

[0074] Therefore, the five-flavor nectar butter ointment of the present invention adopts Chinese medicinal materials and butter as raw materials, and its characteristics are that butter is used as a carrier, and butter is a sticky object, easy to apply, easy to prepare ointment, external use has no adverse reactions, high safety, and the fatty acids contained have the effect of moisturizing the skin and preventing eczema, and the five-flavor nectar butter ointment is more effective in treating eczema than the simple five-flavor nectar treatment. In a word, the five-flavor nectar butter ointment of the present invention is safe and effective and has low toxic and side effects, is gentle and non-irritating to the skin, can be used to treat infantile eczema, and is expected to provide a new ointment for treating skin diseases.

[0075] Although the specific implementation modes of the present invention are described above, those skilled in the art should understand that the specific implementation modes described are only illustrative and are not intended to limit the scope of the present invention. Equivalent modifications and changes made by those skilled in the art in accordance with the spirit of the present invention should be included in the scope of protection of the claims of the present invention.

Claims

1. A five-flavor nectar butter ointment for treating skin diseases, characterized in that: The raw materials and weight portions of the five-flavor nectar butter ointment are as follows: 100 parts of butter, 100 parts of polyethylene glycol, 50 parts of gelatin, 50 parts of stearic acid, 50 parts of Span and 200 parts of five-flavor nectar aqueous extract; The raw materials of the five-flavor nectar water extract are equal weights of ephedra, spatholobus dasyphylla, spatholobus chinensis, arborvitae and cypress branches.

2. The five-flavor nectar butter ointment for treating skin diseases according to claim 1, characterized in that: The preparation method of the five-flavor nectar water extract is as follows: Put 200g of Ephedra, 200g of Pinus dasyphylla, 200g of Rhizoma Cibotii, 200g of Platycladus orientalis and 200g of Cypress branches into a rocking type crusher and grind the medicinal materials through a 40-mesh sieve to obtain medicinal powder; add distilled water to the medicinal powder and heat and boil to extract, with a solid-liquid ratio of 1:10, extract twice, each time for 30-35 minutes, combine the extracts, filter, and concentrate to 1L.

3. A method for preparing a five-flavor nectar butter ointment for treating skin diseases, characterized in that: The preparation method steps are as follows: Step 1: Crush the medicinal materials: Take equal weights of ephedra, scutellaria baicalensis, scutellaria baicalensis, arborvitae and cypress branches, put them into a swing-type crusher and grind them into powder. The powder is sieved through a 40-mesh sieve and the crushing time is 5-10 minutes. Step 2: Extraction: Add distilled water to the powdered medicinal material for heating and boiling extraction, with a solid-liquid ratio of 1:10, and extract twice, each time for 30-35 minutes, combine the extracts, filter, and concentrate to obtain the five-flavor nectar water extract; Step 3, ointment preparation: The following raw materials in parts by weight: 100 parts of butter, 100 parts of polyethylene glycol, 50 parts of gelatin, 50 parts of stearic acid, 50 parts of Span and 200 parts of Schisandra nectar aqueous extract; stir until a milky state with uniform color is formed.

4. The method for preparing the five-flavor nectar butter ointment for treating skin diseases according to claim 3, characterized in that: The raw material dosages of the five-flavor nectar water extract are as follows: 200g of ephedra, 200g of Pinus scoparia, 200g of Rhizoma Cibotii, 200g of Platycladus orientalis and 200g of Cypress branches; after concentration, 1L of the five-flavor nectar water extract is obtained.

5. Use of the Wuwei Ganlu butter ointment for treating skin diseases according to any one of claims 1-2 in the preparation of a medicine for treating skin eczema.

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