Lake sediment eDNA sample low-temperature pollution-free storage and transportation device

Abstract

The invention relates to a low-temperature pollution-free storage and transportation device for lake sediment eDNA samples, and belongs to the technical field of environmental DNA detection. Comprising a container refrigerator, a supporting beam, a sliding seat, a V-shaped supporting roller and a pressing device, the supporting beam is longitudinally installed in the container refrigerator, the sliding seat is installed on the supporting beam in a sliding mode, the V-shaped supporting roller is installed on the sliding seat through a bearing in a free rotating mode, and the pressing device is installed on the sliding seat; the rock core tube and the sample in the rock core tube can be subjected to continuous low-temperature refrigeration and transportation, so that the DNA damage in the transportation process is reduced to the maximum extent, the DNA degradation is slowed down, and the introduction of exogenous DNA pollution in the sample collection and transportation process is reduced; the V-shaped supporting rollers are pushed and pulled to rotate in the loading and unloading process, so that the loading and unloading labor intensity can be effectively reduced; the V-shaped supporting rollers are matched with the pressing device to press and fix the rock core pipe, so that the stability in the transportation process is ensured; the storage and transportation of the rock core pipe sample are facilitated, and the storage time is prolonged.

Description

Technical Field

[0001] The invention belongs to the technical field of environmental DNA detection, and in particular relates to a low-temperature pollution-free storage and transportation device for lake sediment eDNA samples. Background Art

[0002] As an emerging technology used in lake ecosystem monitoring and scientific research, environmental DNA (eDNA) analysis of lake sediments has become a powerful tool in ecology, environmental monitoring, archaeology, paleoecology and paleoenvironmental research due to its non-invasive sampling, high sensitivity, diversity assessment, historical records, wide applicability and high-resolution data, especially in assessing biodiversity and ecosystem health and constructing the history of changes in community composition. The acquisition of high-quality lake sediment eDNA samples has become the basis for reliable analysis, but the current lake sediment cores are stored at room temperature for a long time in the field after collection, the samples are transported together with the sampling equipment, and the temperature rise caused by the defrosting and heating operation of the cold storage during the sample storage process does not take into account the special needs of lake sediment eDNA analysis sample storage. Long-term exposure to polluted environment and sample disturbance and mixing caused by no fixed transportation make lake sediment eDNA face adverse effects such as the introduction of exogenous DNA contamination and accelerated DNA degradation. Therefore, it is urgent to design a set of low-temperature and pollution-free storage and transportation equipment for lake sediment eDNA samples in response to the special needs of continuous low temperature in the field and no introduction of exogenous DNA.

[0003] Environmental DNA is related to the adsorption of DNA by environmental media, the release and degradation rate of DNA in the environment. At the same time, the environmental DNA extraction method and the volume of water samples / sediment samples will also affect the concentration of environmental DNA. If you want to truly reflect the DNA in the collected water / sediment environment, the premise is to minimize the changes that occur after the sample leaves the collection environment. In order to minimize the above changes, certain protective measures are generally taken for the samples, such as refrigeration, freezing or adding stabilizers; then, the transportation time is shortened as much as possible and the analysis and determination are carried out as soon as possible. However, in actual situations, after the environmental samples are collected, they need to be transported to the testing agency. The instability of the transportation environment can easily cause sample contamination, cross-contamination between different layers of sediment, and frequent temperature fluctuations, which can cause DNA degradation and affect the accuracy of the test; in the prior art, samples are stored in core tubes, but there is a lack of devices that can stably support, store and transport the core tubes, which is not conducive to the transportation and long-term preservation of samples stored in the core tubes. Summary of the invention

[0004] In order to overcome the problems in the background technology, the present invention provides a low-temperature and pollution-free storage and transportation device for lake sediment eDNA samples; it can continuously refrigerate and transport the core tube and the samples inside it, prevent DNA degradation, reduce DNA damage during transportation, avoid DNA degradation, maintain DNA integrity and reduce the introduction of exogenous DNA contamination during sample collection and transportation; during the loading and unloading process, the V-shaped support rollers of the core tube are pushed and pulled as they rotate, which can effectively reduce the labor intensity of loading and unloading; the core tube is fixed by the V-shaped support rollers in cooperation with the clamping device to ensure stability during transportation; it is beneficial to the storage and transportation of core tube samples and prolongs the storage time.

[0005] To achieve the above-mentioned purpose, the present invention is implemented through the following technical scheme: the low-temperature and pollution-free storage and transportation device for lake sediment eDNA samples mainly includes a container refrigerator 1, a support beam 2, a sliding seat 3, a V-shaped support roller 4, and a clamping device 5. The support beam 2 is longitudinally installed in the container refrigerator 1, the sliding seat 3 can be slidably installed on the support beam 2, the V-shaped support roller 4 is freely rotatably installed on the sliding seat 3 through a bearing, and the clamping device 5 is installed on the sliding seat 3.

[0006] Furthermore, the clamping device 5 includes a supporting column 51, a pressure plate 52, a clamping nut 53, and an arc-shaped pressure block 54. The supporting column 51 is installed on both sides of the sliding seat 3, the pressure plate 52 is installed on the supporting column 51 through a light hole, the clamping nut 53 is threadedly connected to the top of the supporting column 51, the clamping nut 53 is above the pressure plate 52, and the arc-shaped pressure block 54 matching the core tube is installed at the bottom of the pressure plate 52.

[0007] Furthermore, the arc-shaped pressing block 54 is an arc-shaped elastic clip; a hand wheel for adjusting the force is fixedly mounted on the clamping nut 53 .

[0008] Furthermore, a return spring 55 is sleeved on the support column 51 , and two ends of the return spring 55 are supported on the shoulder of the support column 51 and the bottom of the pressure plate 52 respectively.

[0009] Furthermore, the sliding seats 3 at both ends of the support beam 2 are provided with axial limit devices 6 for supporting the ends of the core tube.

[0010] Furthermore, the axial limiting device 6 includes a vertical plate 61, a baffle 62, and a limiting sleeve 63. The vertical plate 61 is upright mounted on the sliding seat 3. The vertical plate 61 is provided with a through hole 64 for the core tube to pass through. The vertical plate 61 is provided with a slot 65 for inserting the baffle 62. The baffle 62 is inserted and limited in the slot 65. The limiting sleeve 63 that supports the end of the core tube is in the through hole 64.

[0011] Furthermore, a support plate 67 is installed in the limiting sleeve 63 via a spring 66, and a handle 68 for easy operation is provided on the baffle 62.

[0012] Furthermore, the support beam 2 is provided with a longitudinal T-shaped support portion 21, a slide groove 22 is formed between the T-shaped support portions 21, and a protrusion 31 matching the slide groove 22 to ensure smooth sliding is provided at the bottom of the sliding seat 3. A sliding block 23 is provided in the slide groove 22, and the sliding block 23 is installed on the sliding seat 3 by bolts.

[0013] Furthermore, multiple layers and multiple rows of support beams 2 are installed on both sides of the container refrigerator 1, 3 to 5 sliding seats 3 are installed on the support beams 2, and 2 to 5 V-shaped grooves are provided on the V-shaped support rollers 4.

[0014] Beneficial effects of the present invention: The present invention can continuously perform low-temperature refrigeration and transportation of the core tube and the samples inside the core tube, prevent DNA degradation, reduce DNA damage during transportation, avoid DNA degradation, maintain DNA integrity and reduce the introduction of exogenous DNA contamination during sample collection and transportation; the core tube is supported by V-shaped support rollers, and the V-shaped support rollers rotate when pushing and pulling the core tube during loading and unloading, which can effectively reduce the loading and unloading labor intensity; the core tube is fixed by the V-shaped support rollers in cooperation with the clamping device, so as to ensure stability during transportation; it is beneficial to the storage and transportation of the core tube samples and prolongs the storage time. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a three-dimensional schematic diagram of the present invention.

[0016] Figure 2 It is a three-dimensional schematic diagram of the main axle measurement of the present invention.

[0017] Figure 3 It is a three-dimensional schematic diagram of the support beam, sliding seat, V-shaped support roller, clamping device and axial limit device of the present invention.

[0018] Figure 4 yes Figure 3 Schematic diagram of the local enlarged structure at point A in the middle.

[0019] Figure 5 yes Figure 3 Schematic diagram of the local enlarged structure at point B in the middle.

[0020] Figure 6 It is a three-dimensional schematic diagram of the sliding seat, V-shaped support roller, clamping device and axial limiting device of the present invention.

[0021] Figure 7 It is a schematic diagram of the support beam and sliding seat installation structure of the present invention.

[0022] Figure 8 It is a schematic diagram of the structure of the limiting sleeve of the present invention. DETAILED DESCRIPTION

[0023] In order to make the objectives, technical solutions and beneficial effects of the present invention more clear, the preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings to facilitate understanding by technicians.

[0024] The invention discloses a low-temperature pollution-free storage and transportation device for lake sediment eDNA samples. The low-temperature pollution-free storage and transportation device for lake sediment eDNA samples mainly comprises a container refrigerator 1, a support beam 2, a sliding seat 3, a V-shaped support roller 4, and a clamping device 5. The support beam 2 is longitudinally installed in the container refrigerator 1, the sliding seat 3 can be slidably installed on the support beam 2, the V-shaped support roller 4 can be freely rotatably installed on the sliding seat 3 through a bearing, and the clamping device 5 is installed on the sliding seat 3; the core tube and the samples inside thereof can be continuously refrigerated and transported at low temperatures, DNA degradation can be prevented, DNA damage during transportation can be reduced, DNA degradation can be avoided, DNA integrity can be maintained, and introduction of exogenous DNA contamination during sample collection and transportation can be reduced; the core tube is supported by the V-shaped support roller 4, and the V-shaped support roller 4 rotates when the core tube is pushed and pulled during loading and unloading, which can effectively reduce the labor intensity of loading and unloading; the core tube is clamped and fixed by the V-shaped support roller 4 in cooperation with the clamping device 5 to ensure stability during transportation; it is beneficial to the storage and transportation of the core tube samples and prolongs the storage time.

[0025] The clamping device 5 includes a supporting column 51, a pressure plate 52, a clamping nut 53, and an arc-shaped pressure block 54. The supporting column 51 is installed on both sides of the sliding seat 3, the pressure plate 52 is installed on the supporting column 51 through a light hole, the clamping nut 53 is threadedly connected to the top of the supporting column 51, the clamping nut 53 is above the pressure plate 52, and the arc-shaped pressure block 54 matching the core tube is installed at the bottom of the pressure plate 52; the pressure plate 52 is clamped by the clamping nut 53 to ensure that the arc-shaped pressure block 54 clamps the core tube, and the core tube can be radially limited with the support of the V-shaped support roller 4; the structure is simple and the operation is convenient.

[0026] The arc-shaped pressing block 54 is an arc-shaped elastic clip to ensure the pressing effect; a hand wheel for adjusting the force is fixedly installed on the pressing nut 53.

[0027] The support column 51 is sleeved with a return spring 55, and the two ends of the return spring 55 are respectively supported on the shoulder of the support column 51 and the bottom of the pressure plate 52. The return spring 55 can ensure that the pressure plate 52 rises with the clamping nut 53, leaving a passage for the core tube, avoiding dryness during loading and unloading, facilitating loading and unloading work, and effectively improving work efficiency.

[0028] The sliding seats 3 at both ends of the support beam 2 are provided with axial limit devices 6 for supporting the ends of the core tube. The axial limit devices 6 are used to limit the core tube axially to ensure the stability of the core tube.

[0029] The axial limiting device 6 includes a vertical plate 61, a baffle 62, and a limiting sleeve 63. The vertical plate 61 is upright mounted on the sliding seat 3. The vertical plate 61 is provided with a through hole 64 for the core tube to pass through. The vertical plate 61 is provided with a slot 65 for inserting the baffle 62. The baffle 62 is inserted and limited in the slot 65. The limiting sleeve 63 supporting the end of the core tube is in the through hole 64. After the core tube is loaded, the baffle 62 is inserted into the slot 65, and the position of the sliding seat 3 at the upper end of the support beam 2 is adjusted so that the limiting sleeve 63 is supported on the end of the core tube, and the sliding seat 3 is fixed to ensure the reliability of the axial limiting.

[0030] A support plate 67 is installed in the limiting sleeve 63 through a spring 66, and a handle 68 for easy operation is provided on the baffle 62. During the tightening process, the end of the core tube is supported by the elastic supporting force of the spring 66 to ensure the reliability of the support and avoid collision caused by the formation of a gap.

[0031] The support beam 2 is provided with a longitudinal T-shaped support portion 21, and a slide groove 22 is formed between the T-shaped support portions 21. A protrusion 31 matching the slide groove 22 to ensure smooth sliding is provided at the bottom of the sliding seat 3. A slider 23 is provided in the slide groove 22. The slider 23 is installed on the sliding seat 3 by bolts. The position of the sliding seat 3 can be adjusted by adjusting the loosening bolts, and the position of the sliding seat 3 can be fixed by adjusting the tightening bolts.

[0032] The container refrigerator 1 is provided with multi-layer and multi-row support beams 2 on both sides, 3 to 5 sliding seats 3 are installed on the support beams 2, and 2 to 5 V-shaped grooves are provided on the V-shaped support rollers 4, which can improve the refrigeration and transportation capacity, avoid collisions between core tubes, and take into account the convenience of loading and unloading.

[0033] Working process: Loading work: open the door of the container refrigerator 1, easily remove the baffle 62 and the limiting sleeve 63 through the handle 68, adjust the clamping nut 53, and under the elastic force of the return spring 55, the pressure plate 52 rises along with the clamping nut 53, leaving a passage for the core tube to avoid dryness during loading and unloading; insert one end of the core tube through the through hole 64, so that the core tube is supported in the V-groove of the corresponding V-shaped support roller 4, and the V-shaped support roller 4 rotates with the insertion of the core tube to ensure that the core tube is pushed forward with effort. After the core tube is pushed in, insert the baffle 62 into the slot 65, adjust the position of the outermost sliding seat 3 on the support beam 2, so that the limiting sleeve 63 is supported on the core tube The end portion of the core tube is supported by the elastic supporting force of the spring 66 to ensure the reliability of the support and avoid collision caused by the formation of a gap. The clamping nut 53 is adjusted to compress the return spring 55 while pressing the pressing plate 52 downward to press the arc-shaped elastic clip on the core tube to limit the core tube radially and ensure the stability of the core tube. The door of the container refrigerator 1 is closed and the container refrigerator 1 is used for continuous low-temperature storage without defrosting. The temperature is kept at 0-4°C during storage. During transportation, the storage battery or generator is used for power supply. For outdoor power supply, multiple power supply modes such as civil power and power generation are considered.

[0034] Unloading work: open the door of the container refrigerator 1, adjust the clamping nut 53, and under the elastic force of the return spring 55, the pressure plate 52 rises along with the clamping nut 53, and the arc-shaped pressure block 54 is separated from the contact with the core tube; loosen the fastening bolts of the slider 23, adjust the sliding seat 3 to slide outward, ensure that the limiting sleeve 63 is separated from the end of the core tube, and easily remove the baffle 62 and the limiting sleeve 63 through the handle 68, and pull out the core tube. As the core tube is pulled out, the V-shaped support roller 4 rotates to ensure that the core tube is pulled out with less effort. After the core tube is unloaded, the baffle 62 is inserted into the slot 65.

[0035] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined by the claims of the present invention.

Claims

1. A low-temperature pollution-free storage and transportation device for lake sediment eDNA samples, characterized by: The low-temperature pollution-free storage and transportation device for lake sediment eDNA samples comprises a container refrigerator (1), a support beam (2), a sliding seat (3), a V-shaped support roller (4), and a clamping device (5), wherein the support beam (2) is longitudinally installed in the container refrigerator (1), the sliding seat (3) can be slidably installed on the support beam (2), the V-shaped support roller (4) can be freely rotatably installed on the sliding seat (3) through a bearing, and the clamping device (5) is installed on the sliding seat (3).

2. The low-temperature pollution-free storage and transportation device for lake sediment eDNA samples according to claim 1, characterized in that: The clamping device (5) comprises a supporting column (51), a pressure plate (52), a clamping nut (53), and an arc-shaped pressure block (54). The supporting column (51) is installed on both sides of the sliding seat (3). The pressure plate (52) is installed on the supporting column (51) through a light hole. The clamping nut (53) is threadedly connected to the top of the supporting column (51). The clamping nut (53) is located above the pressure plate (52). The arc-shaped pressure block (54) matching the core tube is installed at the bottom of the pressure plate (52).

3. The low-temperature pollution-free storage and transportation device for lake sediment eDNA samples according to claim 2, characterized in that: The arc-shaped pressing block (54) is an arc-shaped elastic clip; a hand wheel for adjusting the force is fixedly mounted on the clamping nut (53).

4. The low-temperature pollution-free storage and transportation device for lake sediment eDNA samples according to claim 2 or 3, characterized in that: The support column (51) is sleeved with a return spring (55), and the two ends of the return spring (55) are respectively supported on the shaft shoulder of the support column (51) and the bottom of the pressure plate (52).

5. The low-temperature pollution-free storage and transportation device for lake sediment eDNA samples according to claim 4, characterized in that: The sliding seats (3) at both ends of the support beam (2) are provided with axial limit devices (6) for supporting the ends of the core tube.

6. The low-temperature pollution-free storage and transportation device for lake sediment eDNA samples according to claim 5, characterized in that: The axial limiting device (6) comprises a vertical plate (61), a baffle plate (62), and a limiting sleeve (63); the vertical plate (61) is upright mounted on the sliding seat (3); a through hole (64) is provided on the vertical plate (61) for the core tube to pass through; a slot (65) is provided on the vertical plate (61) for inserting the baffle plate (62); the baffle plate (62) is inserted and limited in the slot (65); and the limiting sleeve (63) for supporting the end of the core tube is located in the through hole (64).

7. The low-temperature pollution-free storage and transportation device for lake sediment eDNA samples according to claim 6, characterized in that: A support plate (67) is installed in the limiting sleeve (63) via a spring (66), and a handle (68) for easy operation is provided on the baffle (62).

8. The low-temperature pollution-free storage and transportation device for lake sediment eDNA samples according to any one of claims 1-3 and 5-7, characterized in that: The support beam (2) is provided with a longitudinal T-shaped support portion (21), a slide groove (22) is formed between the T-shaped support portions (21), a protrusion (31) matching the slide groove (22) for ensuring smooth sliding is provided at the bottom of the sliding seat (3), a sliding block (23) is provided in the slide groove (22), and the sliding block (23) is mounted on the sliding seat (3) by bolts.

9. The low-temperature pollution-free storage and transportation device for lake sediment eDNA samples according to claim 8, characterized in that: The container refrigerator (1) is provided with multi-layer and multi-row support beams (2) on both sides, 3 to 5 sliding seats (3) are installed on the support beams (2), and 2 to 5 V-shaped grooves are provided on the V-shaped support rollers (4).

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