Separation method of ovarian cancer related mesenchymal stem cells and application of ovarian cancer related mesenchymal stem cells in tumor resistance

Through direct tissue block isolation method and multi-step identification process, ovarian cancer-related mesenchymal stem cells were successfully isolated and identified, solving the problem of lack of consistency standards in the prior art, and providing an effective tool for drug screening and tumor research.

CN119530142AActive Publication Date: 2025-02-28BEIJING UNIV OF CHINESE MEDICINE
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Patent Information

Application Number
CN202411763001.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-03
Publication Date
2025-02-28
Estimated Expiration
2044-12-03

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to a separation method of ovarian cancer related mesenchymal stem cells and application of the ovarian cancer related mesenchymal stem cells in tumor resistance. The separation method comprises the following steps: (S1) extracting and separating mesenchymal stem cells from a fresh tumor sample of an ovarian cancer patient by adopting a direct tissue block method; and (S2) screening and confirming that the mesenchymal stem cells are tumor-derived mesenchymal stem cells through cellular morphology identification, surface marker analysis, iterative stemness detection and tumor and / or fibroblast marker exclusion experiments in sequence. Experiments prove that the ovarian cancer related mesenchymal stem cells can be used for evaluating the anti-tumor efficacy, the anti-invasion ability supported by a tumor matrix and / or the anti-balling ability supported by the tumor matrix of drugs.
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Citation Information

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