Space detection device and method for marking plant leaf extension

Abstract

The invention discloses a space detection device and method for marking expansion of plant leaves. The device comprises a fixed-specification lossless marking grid plate, pigment and a mist spraying tank, the fixed-specification lossless mark grid plate comprises a plurality of fixed-distance grids (6), and gaps between the adjacent fixed-distance grids (6) are mist spraying holes (5). According to the method, a fixed-specification lossless marking grid plate is adopted as a template for food coloring mist spraying marking of the template, the expansion rate of the leaf part can be caught and measured in a fine mode, the method is convenient and fast, the method is easier to carry in a field for use, the operable space is larger, and the price is low. The space detection method for marking plant leaf expansion is lossless, efficient, simple in operation, simple in design and low in cost, and can be used as an experimental consumable; the method is of great significance in promoting simplification of crop leaf real-time expansion phenotypic statistical equipment, improvement of scientific research resource utilization efficiency, acceleration of crop leaf phenotypic characters and reduction of scientific research cost.

Description

Technical Field

[0001] The invention relates to the field of plant science and technology, and in particular to a spatial detection device and method for marking plant leaf expansion. Background Art

[0002] Leaves are the main organs of plants for photosynthesis and transpiration cooling. The detection of leaf-related parameters is not only very important in the fields of genetics, physiology, plant breeding and developmental biology, but also crucial for explaining the energy balance and water economy in the distribution of plant species, plant productivity and plant-environment interactions in a broader sense. Leaf expansion growth is one of the most important parameters in the field of plant science. It is of great significance for obtaining indicators such as crop photosynthetic rate, growth status, physiological and biochemical indicators, crop yield and quality. Many methods have been developed to detect the differential expansion rate of different parts of the leaf.

[0003] Among the many topics in botany, an accurate understanding of the growth of plant organs is important not only in the study of morphogenesis, but also in applied plant science, as leaf expansion is an important factor in plant yield. Traditionally, the measurement of leaf expansion rate is mainly through digital imaging and manual marking. Since the leaves need to be tracked non-destructively, the method of tracking the leaf's own marks (veins) through digital imaging requires a complex setup, in which the leaves must be kept fixed and in a state of tension during measurement. The low resolution makes it difficult to find the leaf's own marks (veins), especially in young leaves. When using the manual marking method, small, non-invasive marks need to be placed on the surface of the leaf, and the position of these marks needs to be restored after a period of time. It is easy to cause errors in the position of the marks in repeated observations, especially in young leaves. At present, some researchers have carried out pioneering research on recording leaf expansion by marking grids on developing tobacco leaves with India ink. However, this technique is only suitable for relatively large leaves, usually requiring the leaves to grow to 6cm, and the initial spacing of the marks is quite rough (about 5mm). A modern variant of this technique uses randomly walking graphite particles for marking and has been used many times. Some researchers have also used non-contact inkjet printing to print small dots (diameter 0.1-0.2mm) on the surface of the leaves to detect the expansion rate of the leaves and the expansion rate of different parts of the leaves. This technology is more accurate than other research measurements, but it requires careful image analysis to reconstruct the elongation marks during the expansion process, and currently only the elongation in one direction has been studied.

[0004] In general, the existing methods for marking plant leaf expansion detection are mainly digital imaging, manual marking, ink marking and inkjet printing. The digital imaging method is complex to set up, difficult to operate, and limited by resolution. Since the leaves need to maintain tension during measurement, it is easy to damage the leaves, and young leaves cannot be measured. The manual marking method is time-consuming and has large errors. There is also the problem that young leaves cannot be marked. The ink marking method is inexpensive, but due to the influence of manual marking, the marking spacing is large, the size of the leaves that can be marked is severely limited, and smaller leaves cannot be marked. The inkjet printing method has high precision and fast speed, but the instrument is expensive, inconvenient to maintain and carry, and image analysis is relatively inconvenient and time-consuming.

[0005] Therefore, how to conveniently and accurately mark the expansion of young leaves of plants has become a problem that people are concerned about.

[0006] In order to solve the above problems, the present invention is proposed. Summary of the invention

[0007] The present invention aims to overcome the deficiencies of the prior art. The present invention discloses a spatial detection device and method for marking plant leaf expansion. The device includes a fixed-specification lossless marking grid plate, a pigment and a mist spray can; the fixed-specification lossless marking grid plate includes a plurality of fixed-distance squares 6, and the gaps between adjacent fixed-distance squares 6 are mist spray pores 5. The present invention uses a fixed-specification lossless marking grid plate as a template for edible pigment mist spray marking templates. This method can accurately capture and measure the expansion rate of leaf parts, and this method is convenient and easier to carry in the field, with a larger operating space and low price. The spatial detection method for marking plant leaf expansion of the present invention is non-destructive and efficient, simple to operate, concise in design, and low in cost. It can be used as an experimental consumable, which is of great significance to promoting the streamlining of crop leaf real-time expansion phenotypic statistical equipment, improving the utilization efficiency of scientific research resources, accelerating crop leaf phenotypic traits, and reducing scientific research costs.

[0008] The present invention adopts the following technical solutions:

[0009] A first aspect of the present invention provides a spatial detection device for marking plant leaf expansion, which includes a fixed-size non-destructive marking grid plate, a pigment, and a mist spray can;

[0010] The fixed-specification lossless marking grid plate includes a plurality of fixed-distance grids 6 , and the gaps between adjacent fixed-distance grids 6 are mist spray pores 5 .

[0011] Preferably, the top 3 of the fixed distance grid and the bottom 4 of the fixed distance grid are both squares, and the side length of the bottom square is greater than the side length of the top square. After mist spraying, the mist spraying coloring distance of the bottom of the edible coloring solution is the length of the bottom 4 of the fixed distance grid.

[0012] Preferably, the width of the top 1 of the mist spray hole is greater than the width of the bottom 2 of the mist spray hole.

[0013] Preferably, the width of the top 1 of the mist spray hole is 0.5-1mm, and the width of the bottom 2 of the mist spray hole is 0.2mm-3mm. More preferably, the width of the top 1 of the mist spray hole is 1mm, and the width of the bottom 2 of the mist spray hole is 0.5mm. The different widths of the top and bottom of the grid edge gap can eliminate the unevenness caused by the spray of the edible pigment solution, so that the bottom of the grid edge gap can be imaged smoothly, reducing the errors in the later image processing and PhotoShop measurement.

[0014] Preferably, the height from top to bottom is 0.5 cm to 1 cm, and the height can be adjusted according to actual conditions.

[0015] Preferably, the pigment comprises edible pigments of different colors, and the color scale value of the pigment is >E300.

[0016] Preferably, the fixed specification non-destructive marking grid plate, the fixed distance grid 6 gap image coloring area is 4-9mm 2 .

[0017] A second aspect of the present invention provides a spatial detection method for marking plant leaf expansion, comprising the following steps:

[0018] S1, placing the fixed-specification non-destructive-marked grid plate on the young leaves of the crop;

[0019] S2, spraying the edible pigment solution at a distance of 10 cm-15 cm from the fixed specification non-destructive marking grid plate; this distance can be adjusted according to actual conditions.

[0020] S3, taking photos and counting the leaf expansion rate according to experimental needs;

[0021] S4, perform Photoshop image processing and analysis on the photos required for the experiment and record the expansion of plant leaves.

[0022] Preferably, in step S4, the PhotoShop image processing and analysis method comprises the following steps:

[0023] 1. In PhotoShop, open the image of the leaf and adjust the leaf to a vertical or horizontal position;

[0024] 2 Use the cropping tool to crop the excess background according to the size of the leaves;

[0025] 3. To facilitate measurement, open the preferences in PhotoShop software to set the unit of the leaf image. After the unit is set successfully, add a ruler to the image to show the actual size of the leaf image.

[0026] 4After adjusting the blade image, click "Window" and select the "Measurement Record" interface;

[0027] 5 Select the "Quick Selection Tool", move the mouse and click the measured leaf area, and click "Record Measurement" to display the size of the measured area;

[0028] 6 Select the data bar and right-click "Export" to obtain a notepad type file to complete the image processing and analysis.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] 1. In terms of detection methods, in view of the shortcomings of the prior art, the spatial detection device and method for marking plant leaf expansion of the present invention comprehensively considers non-destructive marking of crop leaves and facilitates real-time statistics of crop leaf expansion rates. The present invention uses edible pigments as marking colors to reduce the growth impact caused by reagents, and optimizes the conditions of fixed-specification non-destructive marking grid plates to reduce real-time non-destructive marking errors. The expansion rate of crop leaves is more difficult to measure than the outline and area of ​​plants. Whether the various parts of crop leaves expand at the same or different rates during the entire development process, the present invention uses a fixed-specification non-destructive marking grid plate as a template for edible pigment mist spray marking templates. This method is similar to the inkjet printing method and can accurately capture and measure the expansion rate of leaf parts. Compared with inkjet printing, this method is convenient and easier to carry for use in the field, has a larger operating space and is inexpensive.

[0031] 2. In terms of image processing methods, the present invention uses the ruler tool and quick selection tool of PhotoShop to quickly measure the collected real-time expansion photos of crops, which is more convenient and accurate than traditional measurement methods, and is conducive to systematic research on crops. The spatial detection method and image processing technology for marking plant leaf expansion of the present invention are non-destructive and efficient, simple to operate, simple in design, and low in cost, and can be used as experimental consumables. This method is of great significance for promoting the simplification of crop leaf real-time expansion phenotypic statistical equipment, improving the efficiency of scientific research resource utilization, accelerating crop leaf phenotypic traits, and reducing scientific research costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 To mark the local section of the grid plate without loss of fixed specifications;

[0033] Figure 2 Non-destructive marking of grid plate top view for fixed specifications;

[0034] Figure 3This is a diagram of the image processing process.

[0035] 1- top of the mist spray hole, 2- bottom of the mist spray hole, 3- top of the fixed distance grid, 4- bottom of the fixed distance grid, 5- mist spray hole, 6- fixed distance grid. DETAILED DESCRIPTION

[0036] Although the present invention has been described with reference to several typical embodiments, it should be understood that the terms used are illustrative and exemplary, rather than restrictive. Since the present invention can be embodied in a variety of forms without departing from the spirit or essence of the invention, it should be understood that the above embodiments are not limited to any of the foregoing details, but should be interpreted broadly within the spirit and scope defined by the appended claims, so all changes and modifications falling within the scope of the claims or their equivalents should be covered by the appended claims.

[0037] The present invention is further described below by way of examples, which are not limited to the examples. Experimental methods without specific conditions specified in the examples are generally carried out under conventional conditions and conditions described in the manual, or under conditions recommended by the manufacturer. The general equipment, materials, reagents, etc. used are all commercially available unless otherwise specified.

[0038] The specific steps of the method of the present invention are as follows:

[0039] Embodiment 1:

[0040] like Figure 1-2 As shown, the spatial detection device for marking plant leaf expansion in this embodiment includes a fixed-specification non-destructive marking grid plate, a pigment, and a mist spray can;

[0041] The fixed-specification lossless marking grid plate includes a plurality of fixed-distance grids 6 , and the gaps between adjacent fixed-distance grids 6 are mist spray pores 5 .

[0042] The fixed distance grid top 3 and the fixed distance grid bottom 4 are both squares, and the side length of the bottom square is greater than the side length of the top square.

[0043] The width of the top 1 of the mist spray hole is greater than the width of the bottom 2 of the mist spray hole.

[0044] The width of the top 1 of the mist spray hole is 1 mm, and the width of the bottom 2 of the mist spray hole is 0.5 mm. The different widths of the top and bottom of the grid edge gap can eliminate the unevenness caused by the spray of edible pigment solution, so that the bottom of the grid edge gap can be imaged smoothly, reducing the errors of later image processing and PhotoShop measurement.

[0045] The height from top to bottom is 0.5cm.

[0046] The pigment comprises food pigments of different colors, and the color scale value of the pigment is greater than E300.

[0047] Fixed specification non-destructive marking grid plate, the coloring area of ​​the fixed distance grid 6 is 4mm 2 .

[0048] A spatial detection method for marking plant leaf expansion comprises the following steps:

[0049] S1, placing the fixed-specification non-destructive-marked grid plate on the young leaves of the crop;

[0050] S2, spraying the edible pigment solution at a distance of about 10 cm from the fixed specification non-destructive marking grid plate;

[0051] S3, taking photos and counting the leaf expansion rate according to experimental needs;

[0052] S4, perform Photoshop image processing and analysis on the photos required for the experiment and record the expansion of plant leaves.

[0053] like Figure 3 As shown, in step S4, the PhotoShop image processing and analysis method includes the following steps:

[0054] 1. In PhotoShop, open the image of the leaf and adjust the leaf to a vertical or horizontal position;

[0055] 2 Use the cropping tool to crop the excess background according to the size of the leaves;

[0056] 3. To facilitate measurement, open the preferences in PhotoShop software to set the unit of the leaf image. After the unit is set successfully, add a ruler to the image to show the actual size of the leaf image.

[0057] 4After adjusting the blade image, click "Window" and select the "Measurement Record" interface;

[0058] 5 Select the "Quick Selection Tool", move the mouse and click the measured leaf area, and click "Record Measurement" to display the size of the measured area;

[0059] 6 Select the data bar and right-click "Export" to obtain a notepad type file to complete the image processing and analysis.

[0060] The present invention is described above by way of example. It should be noted that, without departing from the core of the present invention, any simple deformation, modification or other equivalent replacement that can be made by those skilled in the art without inventive effort falls within the protection scope of the present invention.

Claims

1. A spatial detection device for marking plant leaf expansion, characterized in that: It includes fixed-size non-destructive marking grid panels, pigments and mist spray cans; The fixed-specification lossless marking grid plate comprises a plurality of fixed-distance square grids (6), and the gaps between adjacent fixed-distance square grids (6) are mist spray pores (5).

2. The spatial detection device for marking plant leaf expansion according to claim 1, characterized in that: The top (3) of the fixed distance square and the bottom (4) of the fixed distance square are both squares, and the side length of the bottom square is greater than the side length of the top square.

3. The spatial detection device for marking plant leaf expansion according to claim 1, characterized in that: The width of the top (1) of the mist spray hole is greater than the width of the bottom (2) of the mist spray hole.

4. The spatial detection device for marking plant leaf expansion according to claim 1, characterized in that: The width of the top (1) of the mist spray hole is 0.5mm-1mm, and the width of the bottom (2) of the mist spray hole is 0.2mm-3mm.

5. The spatial detection device for marking plant leaf expansion according to claim 1, characterized in that: The height from top to bottom is 0.5cm-1cm.

6. The spatial detection device for marking plant leaf expansion according to claim 1, characterized in that: The pigment comprises food pigments of different colors, and the color scale value of the pigment is greater than E300.

7. The spatial detection device for marking plant leaf expansion according to claim 1, characterized in that: Fixed specification non-destructive marking grid plate, the coloring area of ​​the gap between the fixed distance grids (6) is 4-9mm 2 .

8. A spatial detection method for marking plant leaf expansion, characterized in that: It includes the following steps: S1, placing the fixed-specification non-destructive-marked grid plate on the young leaves of the crop; S2, spraying the pigment solution at a distance of 10 cm to 15 cm from the fixed specification non-destructive marking grid plate; S3, taking photos and counting the leaf expansion rate according to experimental needs; S4, perform Photoshop image processing and analysis on the photos required for the experiment and record the expansion of plant leaves.

9. The spatial detection method for marking plant leaf expansion according to claim 8, characterized in that: In step S4, the PhotoShop image processing and analysis method comprises the following steps: ① In PhotoShop software, open the image of the leaf and adjust the leaf to a vertical or horizontal state; ② Use the cropping tool to crop the excess background according to the size of the leaves; ③ Open the preferences in PhotoShop software to set the unit of the leaf image. After the unit is set successfully, add a ruler to the image to show the actual size of the leaf image; ④After adjusting the blade image, click "Window" and select the "Measurement Record" interface; ⑤ Select "Quick Selection Tool", move the mouse and click on the measured blade area, and click "Record Measurement" to display the size of the measured area; ⑥ Select the data bar and right-click "Export" to obtain a notepad type file to complete the image processing and analysis.

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