Personalized nutrition formula recommendation method and system for relieving asthenopia

Abstract

The invention discloses a personalized nutrition formula recommendation method and system for relieving asthenopia, and the method comprises the steps: obtaining an antioxidant index, a relative anti-inflammatory index, an anti-VEGF activity potential index and an improved blood circulation index based on a first raw material through an antioxidant ability algorithm, functional component content calculation, a molecular docking technology and network pharmacology; based on the antioxidant index, the relative anti-inflammatory index, the anti-VEGF activity potential index and the improved blood circulation index, drawing a radar map to obtain various functional indexes of the first raw material; and on the basis of radar map analysis, the comprehensive effect of the first raw material is obtained by combining three dimensions of a composite dietary anti-oxidation index, a relative dietary anti-inflammatory index and a comprehensive nutrition score, and supplements are set by combining the supplement demands of different target crowds. According to the method, various nutrition function calculation methods are integrated, formula design and precise nutrition application of eye protection products are developed, and effectiveness and safety are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of health management, and in particular to a method and system for recommending a personalized nutritional formula for alleviating visual fatigue. Background Art

[0002] Visual fatigue, also known as eye fatigue, is an eye discomfort disease caused by long-term visual tasks and maintaining high attention. The main clinical manifestations are blurred vision, dry eyes, soreness, burning and even decreased vision. Dry eyes and age-related macular degeneration will aggravate the symptoms of visual fatigue. Traditional Chinese medicine believes that visual fatigue mainly has the following four aspects: liver and kidney deficiency, blood loss, and inability to nourish the body; spleen deficiency and weak qi, failure of clear yang to rise, and lack of nourishment for the eyes; fatigue and exhaustion, heart blood deficiency, and lack of nourishment for the eyes; excessive emotional injury, liver depression and qi stagnation, or excessive use of the eyes, qi and blood stasis, resulting in stagnation of the veins, insufficient qi and blood, and no nourishment for the eyes. Therefore, the formula of traditional Chinese medicine to improve visual fatigue often has the effect of nourishing the kidney and blood.

[0003] In recent years, there has been a growing appreciation for the complex relationship between nutrition and eye health. From rare genetic disorders to common metabolic or age-related diseases, it is clear that dietary interventions can have a substantial impact on the progression and management of a wide range of eye diseases.

[0004] With the popularization of electronic devices and the intensification of an aging society, the number of people suffering from visual fatigue is growing. It has become a development trend to develop functional foods that relieve visual fatigue using lutein, anthocyanins, n3 unsaturated fatty acids and other main causes. However, at present, there is no clinical diagnosis and dietary intervention program for subdivided populations for visual fatigue. For example, in the "Merck Manual of Diagnosis and Treatment" and the American Academy of Ophthalmology's discussion on eye health management, although suggestions for improving different vision health problems through nutritional intervention are listed, they are all based on recommendations for nutrients and chemical components, and no practical dietary recommendations have been formed, which is not conducive to practical application. In addition, a variety of nutrients required for eye health, such as vitamin A, β-carotene, and selenium, have a maximum tolerable intake. Excessive intake will lead to negative effects such as increased cancer risk. When recommending nutrients or health foods, dietary nutritional factors should be considered comprehensively so that the level of daily intake after nutritional supplementation is within a safe and effective range.

[0005] Therefore, personalized recommendations for healthy diets based on dietary nutrition data and evaluation algorithms have been one of the focuses of precision nutrition and digital health management research in recent years. However, existing patents mainly focus on overall dietary recommendations for the general population, and only a small number of patents focus on patients with abnormal glucose metabolism, kidney disease, and children, and do not involve vision improvement. Summary of the invention

[0006] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0007] In view of the above existing problems, the present invention is proposed.

[0008] Therefore, the present invention provides a personalized nutritional formula recommendation method and system for relieving visual fatigue to solve the problem that existing eye health management has not formed practical and usable dietary recommendations.

[0009] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0010] In a first aspect, the present invention provides a method for recommending a personalized nutritional formula for alleviating visual fatigue, comprising:

[0011] Based on the first raw material, the antioxidant index, relative anti-inflammatory index, anti-VEGF activity potential index and blood circulation improvement index were obtained through antioxidant capacity algorithm, functional component content calculation, molecular docking technology and network pharmacology;

[0012] Based on the antioxidant index, relative anti-inflammatory index, anti-VEGF activity potential index and blood circulation improvement index, a radar chart is drawn to obtain various functional indicators of the first raw material;

[0013] Based on radar chart analysis, combined with the three dimensions of complex dietary antioxidant index, relative dietary anti-inflammatory index and comprehensive nutritional score, the comprehensive effect of the first raw material is obtained, and supplements are set according to the supplement needs of different target groups.

[0014] As a preferred embodiment of the method for recommending a personalized nutritional formula for relieving visual fatigue described in the present invention, wherein:

[0015] The method of obtaining the antioxidant index by using the antioxidant capacity algorithm comprises the following steps:

[0016] The free radical scavenging efficiency of the test samples was determined by the ABTS algorithm;

[0017] The reducing ability of the stable free radical DPPH of the test sample is determined by the DPPH algorithm;

[0018] The antioxidant activity of the test samples was determined by FRAP algorithm;

[0019] The antioxidant properties of the test samples were determined by the ORAC algorithm;

[0020] The four antioxidant capacity algorithms, ABTS, DPPH, FRAP, and ORAC, were all used with standard samples to establish standard curves;

[0021] Through weighted calculation, the measurement results of the four algorithms were integrated into a comprehensive antioxidant index V1;

[0022] The antioxidant index V1 obtained by weighted calculation is expressed as:

[0023] V1=V ABTS +V DPPH +V FRAP +50×V ORAC / 800.

[0024] As a preferred embodiment of the method for recommending a personalized nutritional formula for relieving visual fatigue described in the present invention, wherein:

[0025] The method of obtaining the relative anti-inflammatory index by calculating the content of the functional components comprises the following steps:

[0026] According to the content of each ingredient in the formula, refer to the relevant database to determine the content of each ingredient;

[0027] Each ingredient was assigned an overall anti-inflammatory effect score;

[0028] The relative anti-inflammatory index is obtained by calculating a weighted value obtained by multiplying the content of the functional component with anti-inflammatory effect in the first raw material by the specific anti-inflammatory effect score;

[0029] The relative anti-inflammatory index V2 is expressed as:

[0030]

[0031] Among them, C i Indicates the content of the i-th ingredient in the formula, G i represents the intake of the ith ingredient in the global average diet, IES i represents the overall anti-inflammatory effect score of the ith ingredient, and n represents the total number of ingredients in the formula.

[0032] As a preferred embodiment of the method for recommending a personalized nutritional formula for relieving visual fatigue described in the present invention, wherein:

[0033] The method of obtaining the anti-VEGF activity potential index by molecular docking technology comprises the following steps:

[0034] Obtain structural information of the main functional ingredients in the formula through non-targeted metabolomics analysis methods;

[0035] The binding energy data reported in the literature and the binding energy data predicted by calculation were comprehensively analyzed.

[0036] For each component, if there are binding energy data reported in the literature, the literature data are used first;

[0037] If literature data were unavailable, computationally predicted binding energy data were used;

[0038] If both literature data and computational prediction data are available, the weighted average of the two is taken;

[0039] The calculation of the predicted binding energy data comprises the following steps:

[0040] Prepare structural files for VEGF protein and small molecules;

[0041] Define the active site and search space of VEGF;

[0042] Use molecular docking software to quickly explore small molecules in the search space;

[0043] Evaluate the comprehensive binding energy BE of small molecules to VEGF;

[0044] The comprehensive binding energy BE is expressed as:

[0045] BE 综合 =w·BE 文献 +(1-w) BE 预测

[0046] Among them, w represents the weight of literature data, and 1-w represents the weight of prediction data;

[0047] According to the comprehensive binding energy BE 综合 , the anti-VEGF activity potential index V3 was calculated according to the following rules;

[0048] If BE is greater than 0, then V3 = 0;

[0049] If BE is between 0 and -15 kcal / mol, then V3 = |BE (kcal / mol)| * 100 / 15;

[0050] If BE is less than -15 kcal / mol, then V3 = (30 - |BE (kcal / mol)|) * 100 / 15.

[0051] As a preferred embodiment of the method for recommending a personalized nutritional formula for relieving visual fatigue described in the present invention, wherein:

[0052] The method of improving the blood circulation index by network pharmacology comprises the following steps:

[0053] Download the structure files of compounds with molecular weight less than 1000Da from the NCBIPubChem database;

[0054] Use the SwissTargetPrediction tool to predict potential biological targets based on the structural information of compounds;

[0055] Use OMIM and GeneCards databases to obtain target information related to dry eye, age-related macular degeneration, visual fatigue, blood circulation and vascular function pathological states;

[0056] Integrate the target information of the compound with the relevant pathological target information obtained from the OMIM and GeneCards databases to form a comprehensive target list;

[0057] Use the KEGG database to map the enrichment of the compound's target in related pathways;

[0058] Identify blood circulation-related pathways that are enriched by the compound combination;

[0059] The number of target points involved in the blood circulation-related pathways is added up to calculate the blood circulation improvement index V4; the blood circulation improvement index V4 is expressed as:

[0060]

[0061] Among them, P i represents the number of targets involved in the ith pathway related to blood circulation, and n represents the total number of pathways related to blood circulation.

[0062] As a preferred embodiment of the method for recommending a personalized nutritional formula for relieving visual fatigue described in the present invention, wherein:

[0063] The step of drawing a radar chart to obtain various functional indicators of the first raw material includes the following steps:

[0064] Quantitatively evaluate V1, V2, V3 and V4 of the first raw material to obtain corresponding values, wherein V2 takes a negative value;

[0065] Based on quantitative indicators, through formula W n =(100-V n ), n = 1, 2, 3, 4 are converted to obtain standardized values ​​and are represented as the lengths of the four axes in the radar chart;

[0066] By calculating the area A of the triangle formed in the four quadrants of the radar chart n , and calculate the corresponding mean and variance;

[0067] By standardizing the value W for each recipe n Sorting and combining the distance analysis between each indicator and the center point of the radar chart to determine the main mechanism of action of the first raw material;

[0068] When comparing similar formulas, the potential value in preventing corresponding ophthalmic diseases was evaluated by analyzing the triangular areas of each in the first, second, and fourth quadrants.

[0069] As a preferred embodiment of the method for recommending a personalized nutritional formula for relieving visual fatigue described in the present invention, wherein:

[0070] The radar chart analysis is based on the three dimensions of the composite dietary antioxidant index, the relative dietary anti-inflammatory index and the comprehensive nutritional score to obtain the comprehensive effect of the first raw material, including:

[0071] The composite dietary antioxidant index includes standardizing the intake of six antioxidant vitamins and minerals, namely vitamin A, vitamin C, vitamin E, selenium, magnesium and zinc, by subtracting the global mean from the individual intake and dividing by the global standard deviation to obtain the standardized intake, and summing the standardized differences of the six antioxidant nutrients to obtain the composite dietary antioxidant index;

[0072] The relative dietary anti-inflammatory index rDII is expressed as:

[0073]

[0074] in, Indicates the anti-inflammatory effects of individual diets, represents the total anti-inflammatory of the average global diet;

[0075] The composite dietary antioxidant index CDAI is expressed as:

[0076]

[0077] Among them, Individual Intake i Indicates individual intake, Mean i Denotes global average, SD i represents the global standard deviation;

[0078] The comprehensive nutritional score NFCn-LIM is expressed as:

[0079]

[0080] Among them, Nutrient i represents the actual intake of the i-th positive nutrient by an individual, DV i Indicates the recommended daily intake of each positive nutrient, Component i represents the actual intake of the i-th functional ingredient by an individual, SPL iIndicates the recommended intake level of each functional ingredient, MRV i It represents the maximum recommended intake of each nutrient with a high risk of overdose, Q represents the specific content of a nutrient, UL i It represents the tolerable upper intake level of each functional ingredient with a high risk of overdose, and ED represents the estimated daily intake of a nutrient or functional ingredient.

[0081] In a second aspect, the present invention provides a personalized nutritional formula recommendation system for alleviating visual fatigue, comprising:

[0082] A bioactivity evaluation module, for obtaining an antioxidant index, a relative anti-inflammatory index, an anti-VEGF activity potential index and an improvement in blood circulation index based on the first raw material through an antioxidant capacity algorithm, functional component content calculation, molecular docking technology and network pharmacology;

[0083] Creating a radar module for drawing a radar chart based on an antioxidant index, a relative anti-inflammatory index, an anti-VEGF activity potential index, and an improved blood circulation index to obtain various functional indicators of the first raw material;

[0084] The comprehensive effect evaluation module is used to obtain the comprehensive effect of the first raw material based on radar chart analysis, combining the three dimensions of complex dietary antioxidant index, relative dietary anti-inflammatory index and comprehensive nutritional score, and set supplements based on the supplement needs of different target groups.

[0085] In a third aspect, the present invention provides a computing device, comprising:

[0086] Memory, used to store programs;

[0087] A processor is used to execute the computer executable instructions, which, when executed by the processor, implement the steps of the personalized nutritional formula recommendation method for relieving visual fatigue.

[0088] In a fourth aspect, the present invention provides a computer-readable storage medium, comprising: when the program is executed by a processor, the steps of implementing the method for recommending a personalized nutritional formula for relieving visual fatigue are implemented.

[0089] Beneficial effects of the present invention: The present invention utilizes a variety of computational methods such as nutrient measurement, molecular docking, and network pharmacology to conduct a comprehensive nutritional evaluation of eye protection products and diets containing eye protection products to improve effectiveness and safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0090] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0091] Figure 1 A basic flow chart of a method for recommending a personalized nutritional formula for relieving visual fatigue provided by one embodiment of the present invention;

[0092] Figure 2 A schematic diagram of calculating a formula for relieving visual fatigue in a method for recommending a personalized nutritional formula for relieving visual fatigue provided by one embodiment of the present invention;

[0093] Figure 3 An enrichment pathway analysis diagram of a method for recommending a personalized nutritional formula for relieving visual fatigue provided by one embodiment of the present invention. DETAILED DESCRIPTION

[0094] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the drawings of the specification. Obviously, the described embodiments are part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary persons in the art without creative work should fall within the scope of protection of the present invention.

[0095] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0096] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0097] The present invention is described in detail with reference to schematic diagrams. When describing the embodiments of the present invention, for the sake of convenience, the cross-sectional diagrams showing the device structure will not be partially enlarged according to the general scale, and the schematic diagrams are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included.

[0098] At the same time, in the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "upper, lower, inner and outer" are based on the directions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as limiting the present invention. In addition, the terms "first, second or third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0099] In the present invention, unless otherwise clearly specified and limited, the terms "install, connect, connect" should be understood in a broad sense, for example: it can be a fixed connection, a detachable connection or an integral connection; it can also be a mechanical connection, an electrical connection or a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0100] Example 1

[0101] Reference Figure 1-2 Table 1 is an embodiment of the present invention, which provides a personalized nutritional formula recommendation method for relieving visual fatigue, including:

[0102] S1: Based on the first raw material, antioxidant index, relative anti-inflammatory index, anti-VEGF activity potential index and blood circulation improvement index were obtained through antioxidant capacity algorithm, functional component content calculation, molecular docking technology and network pharmacology;

[0103] In the embodiment of the present application, the antioxidant index is obtained by an antioxidant capacity algorithm, comprising the following steps:

[0104] The free radical scavenging efficiency of the test samples was determined by the ABTS algorithm;

[0105] The reducing ability of the stable free radical DPPH of the test sample is determined by the DPPH algorithm;

[0106] The antioxidant activity of the test samples was determined by FRAP algorithm;

[0107] The antioxidant properties of the test samples were determined by the ORAC algorithm;

[0108] The four antioxidant capacity algorithms, ABTS, DPPH, FRAP, and ORAC, were all used with standard samples to establish standard curves;

[0109] Through weighted calculation, the measurement results of the four algorithms were integrated into a comprehensive antioxidant index V1;

[0110] In the examples of the present application, the determination method of ABTS is:

[0111] Prepare 7.4mM ABTS and 2.6mM potassium persulfate solutions and place them at -20℃ and 4℃ respectively. Mix them in equal amounts before use and dilute them 8 times to make the OD value between 1.1±0.01. Add 20μL of appropriately diluted extract and 190μL of ABTS working solution, shake and place in a dark place for 30min, measure the OD value at 734nm, and the linearity of the standard curve is 50-500μM Trolox (water-soluble VE standard).

[0112]

[0113] In the examples of the present application, the determination method of DPPH is:

[0114] DPPH was dissolved in pure methanol to prepare a 1 mM stock solution and stored at -20°C. The stock solution was diluted 10 times before each use. 200 μl of the appropriately diluted extract and 3.8 ml of 0.1 mM DPPH solution were added. After vortex mixing, the solution was placed in a dark place for 30 min. The OD value was measured at 515 nm. The linearity of the standard curve was 50-500 mM Trolox (VE standard).

[0115]

[0116] In the examples of the present application, the FRAP determination method is:

[0117] TPTZ was dissolved in 40mM HCl to prepare 10mMTPTZ stock solution ①, 20mM FeCl3.6H2O stock solution ② and 300mM acetate buffer (PH=3.6) stock solution ③ prepared with sodium acetate trihydrate and placed at 4°C. Before use, stock solution ③: ①: ② were mixed in a ratio of 10:1:1, heated at 37°C for 30min, 20μL of appropriately diluted extract and 190μL of FRAP working solution were added, and after shaking, it was placed in a dark place for 30min, and the OD value was measured at 593nm. The linearity of the standard curve was 50-500μM Trolox (water-soluble VE standard).

[0118]

[0119] In the examples of the present application, the method for determining ORAC is:

[0120] The microplate reader was preheated to 37°C before the experiment. 20 μL of appropriately diluted samples were added to the black microplate, and 75 mmol / L phosphate buffer (pH 7.4) was used instead of the samples as blanks, and Trolox standard solution was used instead of the samples to make the standard curve. The experiment was carried out in plate mode. After adding 100 μL FL reaction solution (7.8 μmol / L) by the autosampler, it was preheated at 37°C for 5 min; after preheating, 100 μL 221 mmol / L APH reaction solution was quickly added with an 8-channel pipette; medium-speed oscillation was performed for 5 s; the reaction was carried out at 37°C for 90 min, and the fluorescence intensity (Ex: 485 nm; Em: 528 nm) was measured every 1.5 min. The fluorescence decay curve was drawn, and the area under the curve was calculated to obtain the AUC sample, AUC blank, and AUC control. The ORAC value was expressed in Trolox equivalent (μmolTE / mL).

[0121]

[0122] In the examples of the present application, the antioxidant index V1 obtained by weighted calculation is expressed as:

[0123] V1=V ABTS +V DPPH +V FRAP +50×V ORAC / 800.

[0124] In the examples of the present application, the relative anti-inflammatory index is calculated by the functional component content, comprising the following steps:

[0125] According to the content of each ingredient in the formula, the content of each ingredient is determined by referring to the database, as shown in Table 1;

[0126] Each ingredient was assigned an overall anti-inflammatory effect score;

[0127] The relative anti-inflammatory index is obtained by calculating a weighted value obtained by multiplying the content of the functional component with anti-inflammatory effect in the first raw material by the specific anti-inflammatory effect score;

[0128] Table 1 Main parameters of inflammation-related components

[0129]

[0130]

[0131] In the present application example, the relative anti-inflammatory index V2 is expressed as:

[0132]

[0133] Among them, C i Indicates the content of the i-th ingredient in the formula, G irepresents the intake of the ith ingredient in the global average diet, IES i represents the overall anti-inflammatory effect score of the ith ingredient, and n represents the total number of ingredients in the formula.

[0134] In the present embodiment, the anti-VEGF activity potential index is obtained by molecular docking technology, including the following steps:

[0135] Obtain structural information of the main functional ingredients in the formula through non-targeted metabolomics analysis methods;

[0136] The binding energy data reported in the literature and the binding energy data predicted by calculation were comprehensively analyzed.

[0137] For each component, if there are binding energy data reported in the literature, the literature data are used first;

[0138] If literature data were unavailable, computationally predicted binding energy data were used;

[0139] If both literature data and computational prediction data are available, the weighted average of the two is taken;

[0140] The calculation of the predicted binding energy data comprises the following steps:

[0141] Prepare structural files for VEGF protein and small molecules;

[0142] Define the active site and search space of VEGF;

[0143] Use molecular docking software to quickly explore small molecules in the search space;

[0144] Evaluate the comprehensive binding energy BE of small molecules to VEGF;

[0145] Based on the binding energy data, the anti-VEGF activity potential index V3 was calculated according to the following rules;

[0146] If BE is greater than 0, then V3 = 0;

[0147] If BE is between 0 and -15 kcal / mol, then V3 = |BE (kcal / mol)| * 100 / 15;

[0148] If BE is less than -15 kcal / mol, then V3 = (30 - |BE (kcal / mol)|) * 100 / 15.

[0149] In the embodiment of the present application, the blood circulation index is improved by network pharmacology, comprising the following steps:

[0150] Download the structure files of compounds with molecular weight less than 1000Da from the NCBIPubChem database;

[0151] Use the SwissTargetPrediction tool to predict potential biological targets based on the structural information of compounds;

[0152] Use OMIM and GeneCards databases to obtain target information related to dry eye, age-related macular degeneration, visual fatigue, blood circulation and vascular function pathological states;

[0153] Integrate the target information of the compound with the relevant pathological target information obtained from the OMIM and GeneCards databases to form a comprehensive target list;

[0154] Use the KEGG database to map the enrichment of the compound's target in related pathways;

[0155] Identify blood circulation-related pathways that are enriched by the compound combination;

[0156] The number of targets involved in blood circulation-related pathways was added up to calculate the blood circulation improvement index V4;

[0157] In the embodiment of the present application, the blood circulation improvement index V4 is expressed as:

[0158]

[0159] Among them, P i represents the number of targets involved in the ith pathway related to blood circulation, and n represents the total number of pathways related to blood circulation.

[0160] It should be noted that VEGF (vascular endothelial growth factor) is related to angiogenesis, and abnormal angiogenesis may affect vision and cause visual fatigue. Anti-VEGF activity helps maintain normal vascular structure and function, thereby reducing visual fatigue caused by vascular problems.

[0161] It should be noted that blood circulation is essential for eye health. Good blood circulation ensures that eye tissues are adequately supplied with oxygen and nutrients, while also helping to remove metabolic waste. Improving blood circulation helps prevent eye fatigue caused by lack of oxygen or nutrition.

[0162] It should be noted that indicators V1 to V4 are directly or indirectly related to relieving visual fatigue. A first raw material with high antioxidant, anti-inflammatory, anti-VEGF activity and the ability to improve blood circulation can theoretically provide multiple ways to relieve visual fatigue. For example, antioxidant and anti-inflammatory ingredients can directly reduce eye fatigue, while improving blood circulation and anti-VEGF activity help maintain the overall health of the eyes, thereby indirectly alleviating the symptoms of visual fatigue.

[0163] S2: Based on the antioxidant index, relative anti-inflammatory index, anti-VEGF activity potential index and blood circulation improvement index, a radar chart is drawn to obtain various functional indicators of the first raw material;

[0164] In the embodiment of the present application, a radar chart is drawn to obtain various functional indicators of the first raw material, including the following steps:

[0165] Quantitatively evaluate V1, V2, V3 and V4 of the first raw material to obtain corresponding values, wherein V2 takes a negative value;

[0166] Based on quantitative indicators, through formula W n =(100-V n ), n=1,2,3,4 are converted to obtain standardized values ​​and are represented as the lengths of the four axes in the radar chart, W n The larger it is, the more suitable it is for the purpose corresponding to n.

[0167] By calculating the area A of the triangle formed in the four quadrants of the radar chart n , and calculate the corresponding mean and variance. The higher the mean, the higher the broad spectrum and the lower the degree of personalization;

[0168] By standardizing the value W for each recipe n Sorting and combining the distance analysis between each indicator and the center point of the radar chart to determine the main mechanism of action of the first raw material;

[0169] like Figure 2 As shown, when comparing similar formulations, the potential value in preventing corresponding ophthalmic diseases was evaluated by analyzing the triangular areas of each in the first quadrant (dry eye syndrome), the second quadrant (myopia), and the fourth quadrant (age-related macular degeneration).

[0170] S3: Based on radar chart analysis, combined with the three dimensions of complex dietary antioxidant index, relative dietary anti-inflammatory index and comprehensive nutritional score, the comprehensive effect of the first raw material is obtained, and supplements are set based on the supplement needs of different target groups.

[0171] In the embodiment of the present application, based on radar chart analysis, combined with the three dimensions of composite dietary antioxidant index, relative dietary anti-inflammatory index and comprehensive nutritional score, the comprehensive effect of the first raw material is obtained, including:

[0172] In the embodiment of the present application, the composite dietary antioxidant index includes standardizing the intake of six antioxidant vitamins and minerals, namely vitamin A, vitamin C, vitamin E, selenium, magnesium and zinc, by subtracting the global average from the individual intake and dividing by the global standard deviation to obtain the standardized intake, summing the standardized differences of the six antioxidant nutrients to obtain the composite dietary antioxidant index;

[0173] In the examples of the present application, the relative dietary anti-inflammatory index rDII (same product) is expressed as:

[0174]

[0175] in, Indicates the anti-inflammatory effects of individual diets, represents the total anti-inflammatory of the average global diet;

[0176] In the present application example, the composite dietary antioxidant index CDAI is expressed as:

[0177]

[0178] Among them, Individual Intake i Indicates individual intake, Mean i Denotes global average, SD i represents the global standard deviation;

[0179] 1. In the embodiment of the present application, the rich and limited nutrient score V = NFCn-LIM, which specifically means: positive nutrient score of the day - negative nutrient score - excess score. In view of the development needs of nutritional supplements, it mainly involves vitamin A, β-carotene, lutein / zeaxanthin and other carotene precursors, n3-PUFA, B vitamins, vitamin C, vitamin E, zinc, selenium, etc., and is corrected with reference to the calculation method of the Nutritional Quality Index (NQI). It is necessary to consider both the need for nutritional supplements and the hazards caused by excessive intake.

[0180] In the present application example, the comprehensive nutritional score NFCn-LIM is expressed as:

[0181]

[0182] Among them, Nutrient i represents the actual intake of the i-th positive nutrient by an individual, DV i Indicates the daily reference value, Component i represents the actual intake of the i-th functional ingredient by an individual, SPL i Indicates the recommended intake level of each functional ingredient, MRV i It indicates the maximum recommended intake, Q indicates the specific content of a nutrient, UL i It represents the tolerable upper intake level of each functional ingredient with a high risk of overdose, and ED represents the estimated daily intake of a nutrient or functional ingredient.

[0183] In the examples of the present application, when selecting suitable products for the personalized diet of the target population, the dimensions of rich dietary antioxidant index (CDAI), relative anti-inflammatory index (rDII) and comprehensive nutritional score (NFCn-LIM) are included;

[0184] This embodiment also provides a personalized nutritional formula recommendation system for relieving visual fatigue, including:

[0185] A bioactivity evaluation module, for obtaining an antioxidant index, a relative anti-inflammatory index, an anti-VEGF activity potential index and an improvement in blood circulation index based on the first raw material through an antioxidant capacity algorithm, functional component content calculation, molecular docking technology and network pharmacology;

[0186] Creating a radar module for drawing a radar chart based on an antioxidant index, a relative anti-inflammatory index, an anti-VEGF activity potential index, and an improved blood circulation index to obtain various functional indicators of the first raw material;

[0187] The comprehensive effect evaluation module is used to obtain the comprehensive effect of the first raw material based on radar chart analysis, combining the three dimensions of complex dietary antioxidant index, relative dietary anti-inflammatory index and comprehensive nutritional score, and set supplements based on the supplement needs of different target groups.

[0188] Furthermore, it also includes:

[0189] Memory, used to store programs;

[0190] A processor is used to load the program to execute the personalized nutritional formula recommendation method for relieving visual fatigue.

[0191] This embodiment also provides a computer-readable storage medium storing a program, which, when executed by a processor, implements the personalized nutritional formula recommendation method for relieving visual fatigue.

[0192] The storage medium proposed in this embodiment and the personalized nutritional formula recommendation method for relieving visual fatigue proposed in the above embodiment belong to the same inventive concept. The technical details not described in detail in this embodiment can be referred to the above embodiment, and this embodiment has the same beneficial effects as the above embodiment.

[0193] Through the above description of the implementation methods, the technicians in the relevant field can clearly understand that the present invention can be implemented by means of software and necessary general hardware, and of course can also be implemented by hardware, but in many cases the former is a better implementation method. Based on such an understanding, the technical solution of the present invention is essentially or the part that contributes to the prior art can be embodied in the form of a software product, and the computer software product can be stored in a computer-readable storage medium, such as a computer floppy disk, read-only memory (ReadOnly, Memory, ROM), random access memory (RandomAccess Memory, RAM), flash memory (FLASH), hard disk or optical disk, etc., including a number of instructions for a computer device (which can be a personal computer, server, or network device, etc.) to perform the methods of various embodiments of the present invention.

[0194] Example 2

[0195] Refer to Table 2-8 and Figure 3 , which is an embodiment of the present invention, provides a personalized nutritional formula recommendation method for relieving visual fatigue. In order to verify its beneficial effects, scientific demonstration is carried out through specific implementation methods and implementation effects.

[0196] The specific embodiments are as follows:

[0197] 1. Use algorithms to guide the screening / design of core formulas with antioxidant and anti-inflammatory effects

[0198] Regarding visual fatigue and retinal photooxidative damage caused by long-term use of computer screens, photooxidative damage can further lead to inflammatory damage and cell apoptosis, damaging the retina. Currently, external hot compresses are mainly used to accelerate blood circulation, and anthocyanin supplements are taken to exert antioxidant effects. To this end, it is planned to develop a formula that has both blood circulation acceleration and antioxidant and anti-inflammatory effects, with anthocyanins as the core raw material.

[0199] The first step is to select anthocyanin raw materials: There are 4 types of anthocyanin raw materials, as shown in Table 2, which can be used as the source of core formulas. Their main testing indicators are as follows:

[0200] Table 2 Typical anthocyanin sources

[0201]

[0202]

[0203] If a conventional method is used to design a formula with an anthocyanin content of 100 mg, 2.5 g of raw material 1 = 10 g of raw material 2 = 0.37 g of raw material 3 = 10 g of raw material 4 are added, and the four raw materials only differ in dosage. Combined with the determination and calculation of the antioxidant index V1, it is found that the antioxidant capacity of the four raw materials is significantly different (possibly related to the processing technology), so blueberry anthocyanin from supplier A is preferred as the core raw material.

[0204] The second step is to calculate the anti-inflammatory index and further optimize the raw materials, as shown in Table 3.

[0205] Table 3 Calculation table of anti-inflammatory index of raw materials containing anthocyanins

[0206]

[0207] The results showed that when the anthocyanin content was the same, blueberry extract had stronger anti-inflammatory potential than bilberry extract, making it a better candidate raw material; there was not much difference in the anti-inflammatory potential of the two blueberry extracts.

[0208] In combination with V1 and V2, 2.5 g of raw material 1 is preferably used as the core formula with antioxidant and anti-inflammatory potential.

[0209] The third step is to screen the overall formula based on the core formula of blueberry extract. Taking blueberry beverage as an example, the following two formulas are designed.

[0210] Recipe 1: Blueberry tea beverage, as shown in Table 4.

[0211] Table 4 Calculation table of anti-inflammatory index of anthocyanin-containing formula

[0212]

[0213] Recipe 2: Blueberry carrot juice, as shown in Table 5.

[0214] Table 5 Calculation table of anti-inflammatory index of anthocyanin-containing formula

[0215]

[0216] It can be seen that the anti-inflammatory ability provided by Formula 2-1 is greater than that of Formula 1, while Formula 2-2 is inferior to Formula 1. This means that blueberry carrot fruit and vegetable juice can provide a stronger anti-inflammatory effect, and as a juice drink or tea drink, tea-containing drinks are better.

[0217] 2. Use algorithms to screen auxiliary raw materials that improve vascular function

[0218] Age-related macular degeneration (AMD) is an eye disease that affects the vision of the elderly, mainly involving degenerative changes in the central area of ​​the retina, the macula. This disease can be divided into two types: dry and wet. Wet AMD involves abnormal blood vessel growth, namely the formation of choroidal neovascularization (CNV). In the pathological state of wet AMD, overexpression of vascular endothelial growth factor (VEGF) leads to abnormal blood vessel growth. These blood vessels are often fragile and prone to leakage, causing retinal edema and hemorrhage, and ultimately leading to vision loss. Therefore, ingredients with anti-VEGF effects may have a protective effect against wet AMD and can be added as auxiliary raw materials.

[0219] Table 6 Molecular docking binding free energy results of typical plant components and VEGF

[0220]

[0221] As shown in Table 6, based on the molecular docking results with VEGF, it is believed that catechins and anthocyanins have the lowest binding energy, and catechins < anthocyanins. Therefore, if it is necessary to increase the anti-VEGF function for special populations, a combination of anthocyanin-rich raw materials + catechin-rich raw materials can be used, such as blueberry + tea; coffee rich in chlorogenic acid is also an option, but drinking preferences and coffee categories need to be considered.

[0222] 2. Use algorithms to discover supplementary formulas that promote eye health by improving abnormal glucose and lipid metabolism

[0223] Table 7. Number of plant components predicted based on network pharmacology

[0224]

[0225] As shown in Table 7 and Figure 3 As shown in the figure, the results of network pharmacology based on vascular function-related targets and anthocyanins and catechin components show that quercetin and EGCG have the most predicted edges, and their pathways related to eye health include type 2 diabetes, ischemia, vascular sclerosis, etc., suggesting that they are related to the disorders of eye oxygen and blood supply caused by vascular diseases. Therefore, adding raw materials enriched with quercetin, kaempferol, catechins, and epigallocatechin to the aforementioned core formula is suitable for people with abnormal glucose and lipid metabolism accompanied by "eye stroke". It mainly plays an eye protection role by improving glucose and lipid metabolism and blood circulation, and improving blood supply and oxygen supply to the eyes.

[0226] 4. Use algorithms to guide the selection of safe formulas for vitamin A nutritional supplements to ensure the effectiveness and safety of the main ingredients.

[0227] Vitamin A-type nutritional supplements are aimed at people who have insufficient intake of such nutrients, resulting in vision problems. For example, age-related macular degeneration (AMD) can aggravate the symptoms of visual fatigue in the elderly. The Merck Manual recommends the use of dietary supplements to reduce the risk of advanced AMD in patients with unilateral intermediate or advanced age-related macular degeneration. The nutrients recommended for supplementation include: zinc, copper, vitamin C, vitamin E, lutein plus zeaxanthin (for past or current smokers), such as 10 mg lutein / 2 mg zeaxanthin per day, or beta-carotene or vitamin A (for current and past non-smokers). The reason for agreeing on the use of beta-carotene or vitamin A is based on epidemiological studies that have found that excessive intake increases the risk of cancer in smokers. Both lutein and vitamin A have an upper limit on intake as shown in Table 8, which refers to the daily limit on nutrients / functional ingredients obtained from the overall diet.

[0228] Table 8 Recommended and upper limit intake of lutein, zeaxanthin, β-carotene and vitamin A

[0229]

[0230] In the development of products containing these ingredients, the dosage is usually set based on experience, but there is actually a lack of scientific basis for dosage setting. For example, Product 1 is based on safety and concept, and contains 1 mg of lutein or 100 micrograms of retinol equivalents of vitamin A. Product 2 is based on the main appeal of achieving the "rich" health claim, containing 3 mg of lutein or 240 micrograms of retinol equivalents (male) or 210 micrograms of retinol equivalents (female). Product 3 is function-oriented, containing 5 mg of lutein or 450 micrograms of retinol equivalents (male) or 350 micrograms of retinol equivalents (female).

[0231] The following two types of meals are used to represent different groups of people, and appropriate products are selected based on the algorithm:

[0232] Meal 1:

[0233] Breakfast: 150g corn, 1 egg, 200ml milk;

[0234] Lunch: 100g stir-fried carrots, 50g beef, 50g rice;

[0235] Dinner: 100g spinach, 50g fish, 50g rice;

[0236] Fruit: 1.5 oranges (240g), 1 kiwi fruit (70g);

[0237] Meal 2:

[0238] Breakfast: 100g bread, 200ml milk;

[0239] Lunch: 1 KFC chicken burger (300g), 200ml cola, 1 serving of chicken wings (80g);

[0240] Dinner: 1 portion of spaghetti with pesto sauce (150g);

[0241] Fruit: 100g grapes;

[0242] Calculations show that meal 1 contains about 5.6 mg of lutein, 1.4 mg of zeaxanthin, 9.35 mg of β-carotene, and 1426.5 μg of vitamin A (237.75 μg retinol equivalent). Meal 2 contains almost no lutein and zeaxanthin, but contains 0.2 mg of β-carotene and 120 μg of vitamin A (20 μg retinol equivalent).

[0243] The calculation of the comprehensive nutrition score (NFCn-LIM) for the two meal groups shows that the score of the meal group 1 who consumed one serving of product 2 was the highest, while the score decreased when they consumed one serving of product 3. The reason is that they consumed too much lutein or vitamin A. The score of the meal group 2 who consumed one serving of product 3 was greater than one serving of product 2 and greater than one serving of product 1. The reason is that the intake of lutein or vitamin A is seriously insufficient and urgently needs to be supplemented by functional foods. If the two groups of people drink additional sugary drinks, their scores will decrease, which means that while intervening with functional foods, sugar intake should be controlled, especially for the second group of people.

[0244] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A personalized nutritional formula recommendation method for relieving visual fatigue, characterized in that: include: Based on the first raw material, the antioxidant index, relative anti-inflammatory index, anti-VEGF activity potential index and blood circulation improvement index were obtained through antioxidant capacity algorithm, functional component content calculation, molecular docking technology and network pharmacology; Based on the antioxidant index, relative anti-inflammatory index, anti-VEGF activity potential index and blood circulation improvement index, a radar chart is drawn to obtain various functional indicators of the first raw material; Based on radar chart analysis, combined with the three dimensions of complex dietary antioxidant index, relative dietary anti-inflammatory index and comprehensive nutritional score, the comprehensive effect of the first raw material is obtained, and supplements are set according to the supplement needs of different target groups.

2. The personalized nutritional formula recommendation method for relieving visual fatigue according to claim 1, characterized in that: The method of obtaining the antioxidant index by using the antioxidant capacity algorithm comprises the following steps: The free radical scavenging efficiency of the test samples was determined by the ABTS algorithm; The reducing ability of the stable free radical DPPH of the test sample is determined by the DPPH algorithm; The antioxidant activity of the test samples was determined by FRAP algorithm; The antioxidant properties of the test samples were determined by the ORAC algorithm; Standard curves were established for the four antioxidant capacity algorithms, ABTS, DPPH, FRAP, and ORAC, using standard samples; Through weighted calculation, the measurement results of the four algorithms were integrated into a comprehensive antioxidant index V1; The antioxidant index V1 obtained by weighted calculation is expressed as: V1=V ABTS +V DPPH +V FRAP +50×V ORAC / 800。 3. The personalized nutritional formula recommendation method for relieving visual fatigue according to claim 1 or 2, characterized in that: The method of obtaining the relative anti-inflammatory index by calculating the content of the functional components comprises the following steps: According to the content of each ingredient in the formula, refer to the relevant database to determine the content of each ingredient; Each ingredient was assigned an overall anti-inflammatory effect score; The relative anti-inflammatory index is obtained by calculating the weighted value obtained by multiplying the content of the functional component with anti-inflammatory effect in the first raw material by the specific anti-inflammatory effect score; The relative anti-inflammatory index V2 is expressed as: Among them, C i Indicates the content of the i-th ingredient in the formula, G i represents the intake of the ith ingredient in the global average diet, IES i represents the overall anti-inflammatory effect score of the ith ingredient, and n represents the total number of ingredients in the formula.

4. The personalized nutritional formula recommendation method for relieving visual fatigue according to claim 3, characterized in that: The method of obtaining the anti-VEGF activity potential index by molecular docking technology comprises the following steps: Obtain structural information of the main functional ingredients in the formula through non-targeted metabolomics analysis methods; The binding energy data reported in the literature and the binding energy data predicted by calculation were comprehensively analyzed. For each component, if there are binding energy data reported in the literature, the literature data are used first; If literature data were unavailable, computationally predicted binding energy data were used; If both literature data and computational prediction data are available, the weighted average of the two is taken; The calculation of the predicted binding energy data comprises the following steps: Prepare structural files for VEGF protein and small molecules; Define the active site and search space of VEGF; Use molecular docking software to quickly explore small molecules in the search space; Evaluate the comprehensive binding energy BE of small molecules to VEGF; Based on the binding energy data, the anti-VEGF activity potential index V3 was calculated according to the following rules; If BE is greater than 0, then V3 = 0; If BE is between 0 and -15 kcal / mol, then V3 = |BE (kcal / mol)| * 100 / 15; If BE is less than -15 kcal / mol, then V3 = (30 - |BE (kcal / mol)|) * 100 / 15.

5. The personalized nutritional formula recommendation method for relieving visual fatigue according to claim 4, characterized in that: The method of improving the blood circulation index by network pharmacology comprises the following steps: Download the structure files of compounds with molecular weight less than 1000Da from the NCBIPubChem database; Use the SwissTargetPrediction tool to predict potential biological targets based on the structural information of compounds; Use OMIM and GeneCards databases to obtain target information related to dry eye, age-related macular degeneration, visual fatigue, blood circulation and vascular function pathological states; Integrate the target information of the compound with the relevant pathological target information obtained from the OMIM and GeneCards databases to form a comprehensive target list; Use the KEGG database to map the enrichment of the compound's target in related pathways; Identify blood circulation-related pathways that are enriched by the compound combination; The number of targets involved in blood circulation-related pathways was added up to calculate the blood circulation improvement index V4; The improved blood circulation index V4 is expressed as: Among them, P i represents the number of targets involved in the ith pathway related to blood circulation, and n represents the total number of pathways related to blood circulation.

6. The personalized nutritional formula recommendation method for relieving visual fatigue according to claim 5, characterized in that: The step of drawing a radar chart to obtain various functional indicators of the first raw material includes the following steps: Quantitatively evaluate V1, V2, V3 and V4 of the first raw material to obtain corresponding values, wherein V2 takes a negative value; Based on quantitative indicators, through formula W n =(100-V n ), n = 1, 2, 3, 4 are converted to obtain standardized values ​​and are represented as the lengths of the four axes in the radar chart; By calculating the area A of the triangle formed in the four quadrants of the radar chart n , and calculate the corresponding mean and variance; By standardizing the value W for each recipe n Sorting is performed, and the main mechanism of action of the first raw material is determined by combining the distance analysis between each indicator and the center point of the radar chart; When comparing similar formulas, the potential value in preventing corresponding ophthalmic diseases was evaluated by analyzing the triangular areas of each in the first, second, and fourth quadrants.

7. The personalized nutritional formula recommendation method for relieving visual fatigue according to claim 6, characterized in that: The radar chart analysis is based on the three dimensions of the composite dietary antioxidant index, the relative dietary anti-inflammatory index and the comprehensive nutritional score to obtain the comprehensive effect of the first raw material, including: The composite dietary antioxidant index includes standardizing the intake of six antioxidant vitamins and minerals, namely vitamin A, vitamin C, vitamin E, selenium, magnesium and zinc, by subtracting the global mean from the individual intake and dividing by the global standard deviation to obtain the standardized intake, and summing the standardized differences of the six antioxidant nutrients to obtain the composite dietary antioxidant index; The relative dietary anti-inflammatory index rDII is expressed as: in, Indicates the anti-inflammatory effects of individual diets, represents the total anti-inflammatory of the average global diet; The composite dietary antioxidant index CDAI is expressed as: Among them, Individual Intake i Indicates individual intake, Mean i Denotes global average, SD i represents the global standard deviation; The comprehensive nutritional score NFCn-LIM is expressed as: Among them, Nutrient i represents the actual intake of the i-th positive nutrient by an individual, DV i Indicates the daily reference value, Component i represents the actual intake of the i-th functional ingredient by an individual, SPL i Indicates the recommended intake level of each functional ingredient, MRV i It indicates the maximum recommended intake, Q indicates the specific content of a nutrient, UL i It represents the tolerable upper intake level of each functional ingredient with a high risk of overdose, and ED represents the estimated daily intake of a nutrient or functional ingredient.

8. A system based on the personalized nutritional formula recommendation method for relieving visual fatigue according to claim 1, characterized in that: A bioactivity evaluation module, for obtaining an antioxidant index, a relative anti-inflammatory index, an anti-VEGF activity potential index and an improvement in blood circulation index based on the first raw material through an antioxidant capacity algorithm, functional component content calculation, molecular docking technology and network pharmacology; Creating a radar module for drawing a radar chart based on an antioxidant index, a relative anti-inflammatory index, an anti-VEGF activity potential index, and an improved blood circulation index to obtain various functional indicators of the first raw material; The comprehensive effect evaluation module is used to obtain the comprehensive effect of the first raw material based on radar chart analysis, combining the three dimensions of complex dietary antioxidant index, relative dietary anti-inflammatory index and comprehensive nutritional score, and set supplements based on the supplement needs of different target groups.

9. An electronic device, characterized in that: include: Memory, used to store programs; A processor is used to load the program to execute the steps of the personalized nutritional formula recommendation method for relieving visual fatigue as described in any one of claims 1-7.

10. A computer-readable storage medium storing a program, characterized in that: When the program is executed by a processor, the steps of the personalized nutritional formula recommendation method for relieving visual fatigue as described in any one of claims 1 to 7 are implemented.

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