Growth regulator for inhibiting excessive growth of new shoots of grapes and use method of growth regulator

Abstract

The invention relates to the technical field of plant growth regulators, in particular to a growth regulator for inhibiting excessive growth of grape shoots and a use method of the growth regulator. The growth regulator for inhibiting the excessive growth of the new shoots of the grapes is prepared from the following raw materials in parts by mass: 10 to 20 g / L of fulvic acid, 0.2 to 0.4 g / L of exchange acid calcium, 0.15 to 0.45 g / L of D-serine, 1.5 to 2.5 g / L of L-valine, 10 to 20 g / L of compound amino acid powder and the balance of water. The regulator product disclosed by the invention has an obvious effect of inhibiting excessive growth of new shoots of grapes, and the germination rate of side shoots is lower.

Description

Technical Field

[0001] The invention relates to the technical field of plant growth regulators, and in particular to a growth regulator for inhibiting the excessive growth of new grape shoots and a use method thereof. Background Art

[0002] Grape (Vitaceae Vitis L.), as a vine plant, has the characteristics of high germination rate and large growth. Existing data show that the annual growth of grape shoots can reach 1 to 2 meters, and vigorous varieties can grow more than 6 meters a year. During the growth period of grapes, the peak growth period of new shoots is mainly concentrated in the period from germination and leafing to flowering. During this period, the excessive growth of new shoots will not only consume a lot of nutrients from the tree, but also affect the flowering and fruiting of grapes, and even cause a decrease in grape yield; at the same time, the multi-level continuous extraction of grape axillary buds will also increase the amount of grape leaf curtains, seriously shading, and reduce the quality of the fruit. In addition, repeated pruning management measures such as removing side shoots and pinching during the growing season also consume a lot of manpower and material resources. Therefore, there is an urgent need in the market for a growth regulator that effectively inhibits the excessive growth of grape shoots. Summary of the invention

[0003] The purpose of the present invention is to provide a growth regulator for inhibiting the excessive growth of new grape shoots and a method for using the same. The new grape shoot growth regulator of the present invention can not only effectively inhibit the excessive growth of new grape shoots, but also ensure the quality and yield of grape fruits.

[0004] In order to achieve the above objectives, the present invention provides the following technical solutions:

[0005] The present invention provides a growth regulator for inhibiting the excessive growth of new shoots of grapes, which is prepared from the following raw materials in parts by weight:

[0006] Fulvic acid 10-20 parts, calcium fulvic acid 0.2-0.4 parts, D-serine 0.15-0.45 parts, L-valine 1.5-2.5 parts, composite amino acid powder 10-20 parts.

[0007] The present invention also provides a method for using the growth regulator for inhibiting the excessive growth of new grape shoots, comprising the following steps:

[0008] Spray growth regulators during the leaf expansion period of grapes, 2 to 10 times, with an interval of 7 to 10 days between each spraying.

[0009] Preferably, the leaf expansion period is the period when 3 to 4 leaves are expanded.

[0010] Preferably, the dosage for each spraying is 400-600 ml / tree.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] The growth regulator for inhibiting the extension of new shoots of grapes of the present invention is compounded and evenly sprayed on grape leaves during the leaf expansion period of grapes when in use, which can effectively inhibit the excessive growth of new shoots during the growth period of grapes and reduce the nutrients lost due to the excessive growth of new shoots; at the same time, it also reduces the need for repeated pruning management measures such as removing side shoots and pinching during the growing season, thereby reducing the investment of manpower and material resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 The grape leaf phytotoxicity diagram in Example 1;

[0014] Figure 2 The graph is a graph of the internode length (4th to 11th nodes) of grapes in each treatment in Example 4; wherein CK is the control group, 1 is treatment 1, 2 is treatment 2, 3 is treatment 3, and 4 is treatment 4;

[0015] Figure 3 The single grape pictures of each treatment in Example 4; CK is the control group, 1 is treatment 1, 2 is treatment 2, 3 is treatment 3, and 4 is treatment 4;

[0016] Figure 4 This is the grape leaf phytotoxicity picture of the control (CK) in Example 4;

[0017] Figure 5 This is a diagram of the phytotoxicity of grape leaves in treatment 1 in Example 4;

[0018] Figure 6 This is a diagram of the phytotoxicity of grape leaves in treatment 2 in Example 4;

[0019] Figure 7 This is a diagram of the phytotoxicity of grape leaves in treatment 3 in Example 4;

[0020] Figure 8 This is a picture of the phytotoxicity of grape leaves subjected to treatment 4 in Example 4. DETAILED DESCRIPTION

[0021] The technical solution provided by the present invention is described in detail below in conjunction with the examples, but they should not be understood as limiting the scope of protection of the present invention. The composite amino acid powder of the present invention was purchased from Sichuan Mianzhu Ruiyang Biotechnology Co., Ltd., with a product batch number of 212302010201.

[0022] Example 1

[0023] Preliminary screening of the composition and concentration of the growth regulator for inhibiting the excessive growth of new grape shoots according to the present invention

[0024] Five-year-old open-field grape Kyoho was used as the test material (2021). When 3 to 4 leaves were unfolded, 10 trees with consistent growth were selected from each treatment, and 10 new shoots of similar length were selected from each tree for marking, and the length of the marked new shoots was measured. The regulator spraying test was carried out every 7 to 10 days, for a total of 7 sprayings, and the growth of new shoots was measured every 15 days. The dosage for each spraying was 500 ml / tree. Except for the various components in each 500 ml growth regulator, the rest was supplemented with water. SAS software was used to count the optimal growth results 60 days after the initial screening.

[0025] In order to obtain the initial screening formula concentration of growth regulators, a 4-factor 4-level orthogonal experimental L16 (44) design (see Table 1) was adopted, with a total of 16 treatments.

[0026] Table 1 Preliminary screening of composition ratios of growth regulators in different treatments

[0027]

[0028] The test results are shown in Table 2. The growth regulator formula screened initially can effectively inhibit the growth of new grape shoots. Treatments 11 and 8 have a more obvious inhibitory effect on the growth of new grape shoots. The optimal test ingredient formula is treatment 11 (potassium phosphite 2g / L, prohexadione calcium 0.133g / L, D-serine 1.05g / L, L-valine 0.703g / L, and compound amino acid powder 3.33g / L). The average length of grape shoots is 58.4cm, and the growth of new shoots is 39.2cm. Treatment 8 (potassium phosphite 1g / L, prohexadione calcium 0.2g / L, D-serine 0.7g / L, L-valine 0.703g / L) has an average growth of 58.4cm and a new shoot growth of 39.7cm.

[0029] Table 2 Length of new shoots of grapes under different treatments

[0030]

[0031]

[0032] According to the SAS analysis results, the best theoretical regulator formula is 0.2 g / L of calcium prohexadione, 0.703 g / L of L-valine, and 3.33 g / L of compound amino acid powder. The detailed results are shown in Table 3.

[0033] Table 3 Optimal theoretical concentration and influence ranking of each component

[0034]

[0035] From Table 3 above, we can see that:

[0036] Compound amino acid powder: ranked first in terms of influence, with the best concentration of 3.33 g / L (0 g / L, 1.11 g / L, 2.22 g / L, 3.33 g / L). The concentration gradient should be increased in the next step of optimization.

[0037] Calcium prohexadione: The influence is ranked second, and the optimal theoretical concentration is 0.2g / L (0g / L, 0.067g / L, 0.133g / L, 0.2g / L). The concentration gradient can be further refined in the next step of optimization;

[0038] L-valine: The influence ranks third, the optimal theoretical concentration is 0.703g / L (0g / L, 0.703g / L, 1.406g / L, 2.109g / L), according to the analysis of the phytotoxicity (see Figure 1 ), which can reduce the concentration gradient in the next step of optimization;

[0039] D-serine: The fourth in terms of influence, the optimal theoretical concentration is 0g / L (0g / L, 0.35g / L, 7g / L, 1.05g / L). However, in the optimal test formula, the concentration is 1.05g / L. At the same time, according to the analysis of the phytotoxicity, the concentration gradient of this ingredient should be reduced in the next step of optimization;

[0040] Potassium phosphite: It ranks fifth in influence, and its optimal theoretical concentration is 0g / L (0g / L, 1g / L, 2g / L, 3g / L). Considering that the initial screening concentration was low and did not achieve the inhibitory effect, the concentration gradient of this component can be increased in the next step of optimization.

[0041] Example 2

[0042] Optimization of the composition and concentration of the growth regulator for inhibiting the excessive growth of new grape shoots according to the present invention

[0043] In order to optimize the initial screening formula of the growth regulator (based on the TOPSIS analysis results of Example 1 and the occurrence of phytotoxicity), the regulators described in the present invention were compounded according to the ingredients and concentrations in Table 4.

[0044] Five-year-old outdoor grape Kyoho was used as the test material (2022). When 3 to 4 leaves were developed, 10 trees with consistent growth were selected from each treatment, and 10 new shoots of similar length were selected from each tree for marking, and the length of the marked new shoots was measured. A growth regulator spraying test was carried out every 7 to 10 days, for a total of 7 sprayings, and the growth of new shoots was measured every 15 days. The dosage for each spraying was 500 ml / tree. In addition to the various components in each 500 ml growth regulator, the rest was supplemented with water. SAS software was used to calculate the optimal growth results 60 days after the initial screening.

[0045] Table 4 Optimization of growth regulator ratios in different treatments

[0046]

[0047]

[0048] The test results are shown in Table 5. The optimized formula can effectively inhibit the growth of new grape shoots, and treatment 3 has a significant inhibitory effect on the growth of new grape shoots. The optimal test ingredient formula is treatment 3 (0.4 g / L prohexadione calcium, 0.2 g / L D-serine, 0.7 g / L L-valine, and 10 g / L compound amino acid powder), and the average length of new grape shoots is 74 cm.

[0049] Table 5 Grape shoot length after each optimization treatment

[0050]

[0051]

[0052] The best theoretical regulator formula obtained according to the SAS analysis results is potassium phosphite 0g / L, calcium prohexadione 0.2g / L, D-serine 0.2g / L, L-valine 1g / L, and compound amino acid powder 10g / L. Detailed results are shown in Table 6.

[0053] Table 6 Optimal theoretical concentration and influence ranking of each component

[0054]

[0055] From Table 6 above, we can see that:

[0056] Potassium phosphite: It ranks first in influence, and the optimal theoretical concentration is 0g / L (0g / L, 3g / L, 4.5g / L, 5g / L). This shows that this ingredient can increase the length of new shoots of the test material at each concentration set in the experiment, and has the effect of promoting the growth of new shoots. This ingredient can be eliminated in the next step of optimization.

[0057] Compound amino acid powder: ranked second in influence, with the best concentration being 10g / L (0g / L, 8g / L, 10g / L, 12g / L). The concentration gradient can be further refined in the next step of optimization.

[0058] D-serine: The influence is ranked third, and the optimal theoretical concentration is 0.2g / L (0g / L, 0.1g / L, 0.2g / L, 0.3g / L). However, in the optimal test formula, the concentration is 1.05g / L, and the concentration gradient can be further refined in the next step of optimization;

[0059] Prohexadione calcium: The influence is ranked fourth, and the optimal theoretical concentration is 0.2g / L (0g / L, 0.2g / L, 0.4g / L, 0.6g / L). The concentration gradient can be further reduced in the next step of optimization;

[0060] L-valine: Ranked fifth in influence, the optimal theoretical concentration is 1g / L (0g / L, 0.4g / L, 0.7g / L, 1g / L). The concentration gradient of this ingredient can be increased in the next step of optimization.

[0061] Example 3

[0062] The components and concentrations of the growth regulator for inhibiting the excessive growth of new grape shoots of the present invention are further optimized.

[0063] In order to further optimize the formula of the growth regulator, the growth regulator of the present invention is compounded according to the ingredients and concentrations of Table 7, specifically 10-20 g / L of fulvic acid, 0.2-0.4 g / L of calcium fulvic acid, 0.15-0.45 g / L of D-serine, 1.5-2.5 g / L of L-valine, and 10-20 g / L of composite amino acid powder.

[0064] Five-year-old outdoor grape Kyoho was used as the test material (2023). When 3 to 4 leaves were developed, 10 trees with consistent growth were selected in each treatment, and 10 new shoots of similar length were selected on each tree for marking, and the internode length of the marked new shoots was measured. The regulator spraying test was carried out every 7 to 10 days, for a total of 5 sprayings, and the internode length was measured every 15 days, for a total of 3 measurements. The internode length was selected from the 4th to 13th nodes from the base of the new shoot to reduce the error caused by differences in tree vigor. The dosage of each spraying was 500 ml / tree. In addition to the various components in each 500 ml growth regulator, the rest was supplemented with water. After 60 days of the experiment, the optimal growth results were statistically analyzed using SAS software.

[0065] Table 7 Optimization of growth regulator ratios in different treatments

[0066]

[0067] The results of the optimization test are shown in Table 8 below. Treatments 8, 11 and 12 have a significant inhibitory effect on the length of new shoots and internodes of grapes. The total internode length and internode length growth of treatment 8 (10 g / L fulvic acid, 0.4 g / L calcium humate, 0.3 g / L D-serine, 1.5 g / L L-valine) were 86 cm and 48 cm respectively; the total internode length and internode length growth of treatment 11 (15 g / L fulvic acid, 0.3 g / L calcium humate, 0.45 g / L D-serine, 1.5 g / L L-valine, 20 g / L compound amino acid powder) were 93.7 cm and 50 cm respectively; the total internode length and internode length growth of treatment 12 (15 g / L fulvic acid, 0.4 g / L calcium humate, 0.15 g / L D-serine, 15 g / L compound amino acid powder) were 91.7 cm and 51 cm respectively.

[0068] Table 8 Growth status of grape shoots after optimization treatment

[0069]

[0070] Therefore, according to the SAS analysis results, the optimal theoretical regulator formula is 15 g / L fulvic acid, 0.3 g / L calcium fulvic acid, 0.3 g / L D-serine, 1.5 g / L L-valine, and 15 g / L compound amino acid powder; the detailed results are shown in Table 9.

[0071] Table 9 Optimal theoretical concentration and influence ranking of each component

[0072]

[0073]

[0074] Example 4

[0075] Verification of the growth regulator for inhibiting the excessive growth of new grape shoots and investigation of the growth status after drug damage and drug withdrawal according to the present invention

[0076] According to the statistical results of Example 3, a verification test of the regulator formula was carried out. Five-year-old open-field black peak grapes were used as test materials (2024). During the period of 3 to 4 leaves, 10 trees with consistent growth were selected for each treatment, and 10 new shoots of similar length were selected on each tree for marking. A regulator spraying test was carried out every 10 days, for a total of 5 sprayings. The internode length of the grape shoots was measured every 15 days, and the internode length was selected from the 4th to 11th nodes from the base of the shoots to reduce the error caused by differences in tree vigor. The dosage for each spraying was 500ml / tree, and each 500ml growth regulator was supplemented with water except for each component. SAS software was used to statistically analyze the optimal growth results 60 days after the initial screening.

[0077] After the verification test, leaves from each treatment were collected, photographed and observed for drug damage. At the same time, the growth of new shoots was investigated after the verification test, that is, the internode length (4th to 11th nodes) was measured every 10 days after the drug was stopped, and the growth of internode length was calculated. SPSS software was used to statistically analyze the differences between treatments.

[0078] There are five treatments in the experiment, namely control (CK), treatment 1 (optimal test formula of Example 3), treatment 2 (optimal theoretical formula of Example 3), treatment 3 (treatment 11 of Example 3) and treatment 4 (treatment 12 of Example 3). Detailed treatment grouping is shown in Table 10.

[0079] Table 10 Optimization of growth regulator ratios in different treatments

[0080]

[0081] The verification results are shown in Table 11 below. Treatment 2 (optimal theoretical formula) has the best inhibitory effect on the growth of new grape shoots (see Figure 2 The internode length growth of treatment 2 was 19.2 cm, which was 47% lower than that of the control (36.2 cm); the internode length growth of treatments 1, 3, and 4 were 29.6 cm, 26.8 cm, and 22.6 cm, respectively, which were 23%, 26%, and 38% lower than that of the control. The results showed that there was no significant difference between the treatments and the control (see Figure 3 ), indicating that spraying the growth regulator of the present invention did not affect the quality of grape fruit.

[0082] Table 11 Grape fruit quality and new shoot growth status under different treatments

[0083]

[0084] Note: Different lowercase letters in the same column indicate significant differences among treatments (P<0.05).

[0085] It can be seen from Table 11 above that the self-developed grape new shoot inhibition spraying experiment was carried out with Black Peak grapes as the test material, and the optimal theoretical formula (15g / L fulvic acid, 0.3g / L calcium fulvic acid, 0.3g / L D-serine, 1.5g / L L-valine, 15g / L compound amino acid powder) had the best inhibition effect.

[0086] The results of drug damage are as follows Figures 4 to 8 As shown, no phytotoxicity occurred in each treatment, indicating that spraying the growth regulator of the present invention did not affect the normal growth of grape plants. The leaves of grapes in treatments 2 and 4 were significantly smaller than those in the control.

[0087] The changes in internode length (4th to 11th nodes) of grape shoots after drug withdrawal are shown in Table 12. About 20 days after drug withdrawal, new shoots of grapes in some treatments began to resume growth; about 40 days after drug withdrawal, new shoots in all treatments resumed growth, and there was no significant difference between each treatment and the control.

[0088] Table 12 Growth of new shoots after drug withdrawal in different treatments

[0089]

[0090]

[0091] Note: Different lowercase letters in the same column indicate significant differences among treatments (P<0.05).

[0092] In summary, the growth regulator for inhibiting the excessive growth of new shoots of grapes according to the present invention can effectively inhibit the growth of new shoots of grapes. Taking Black Peak grapes as the test material, the best formula is the best theoretical formula obtained by SAS analysis (15 g / L fulvic acid, 0.3 g / L calcium fulvic acid, 0.3 g / L D-serine, 1.5 g / L L-valine, and 15 g / L composite amino acid powder). This also shows that the reasonable application of non-completely orthogonal experiments can achieve good results in the research and development of similar products.

[0093] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A growth regulator for inhibiting the excessive growth of new grape shoots, characterized in that: The method is prepared by comprising the following raw materials in parts by weight: Fulvic acid 10-20 parts, calcium fulvic acid 0.2-0.4 parts, D-serine 0.15-0.45 parts, L-valine 1.5-2.5 parts, composite amino acid powder 10-20 parts.

2. A method for using the growth regulator for inhibiting the excessive growth of new grape shoots according to claim 1, characterized in that: The steps include: Spray growth regulators during the leaf expansion period of grapes, 2 to 10 times, with an interval of 7 to 10 days between each spraying.

3. The method of use according to claim 2, characterized in that: The leaf expansion period is the period when 3 to 4 leaves are expanded.

4. The method of use according to claim 2, characterized in that: The dosage for each spraying is 400-600 ml / tree.

Images