Composition with anti-fatigue effect and preparation method thereof

Abstract

The invention discloses a composition with an anti-fatigue effect and a preparation method thereof. The anti-fatigue composition of the present invention comprises a long-term anti-fatigue component (ginseng fermentation product produced by probiotic fermentation, rich in rare ginsenoside) and a short-term anti-fatigue component (taurine, vitamin B, etc.), and thus can resist fatigue by stimulating body functions for a short period of time, and can relieve fatigue by improving the body state for a long period of time.

Description

Technical Field

[0001] This patent application belongs to the field of biopharmaceutical manufacturing or food biotechnology, and specifically relates to a composition rich in rare ginsenosides with anti-fatigue effect and a preparation method thereof. Background Art

[0002] In the fast-paced modern life, the increasingly fierce social competition makes work and study pressures follow us everywhere, and fatigue has become a health problem that many people cannot get rid of. In order to meet this challenge, many medicines and health foods with fatigue relief and anti-fatigue effects have emerged on the market. These anti-fatigue products are of various types and have different functions. They help people relieve fatigue and restore physical strength through different ingredients and mechanisms. First of all, energy supplement ingredients effectively relieve fatigue by providing the body with nutrients consumed during exercise and maintaining the normal physiological functions of the body. Such products are usually rich in energy substances such as carbohydrates and proteins, which can quickly replenish energy for the body. Secondly, vitamin and trace element supplement ingredients are also a popular choice in the market. When the human body is in a state of fatigue, it often lacks vitamins and trace elements. By supplementing these essential nutrients, the body's metabolic capacity can be enhanced, and the discharge of harmful substances in the body can be promoted, thereby reducing fatigue.

[0003] Most of the existing anti-fatigue products use nutrients such as vitamins, taurine, caffeine, etc. as anti-fatigue ingredients. These products resist fatigue by stimulating body functions in a short period of time, rather than relieving fatigue by improving the body's condition in the long term.

[0004] In recent years, with the development of science and technology and people's pursuit of health, fermented ginseng has gradually attracted attention. Through fermentation, the saponin substances in ginseng increase, which can improve the bioavailability of ginseng and reduce the amount of medicinal materials used. Fermented ginseng can also reduce the dryness caused by long-term use of ginseng, which makes various functional products using fermented ginseng as raw materials more popular with consumers.

[0005] However, the composition of various ginsenosides produced by biological fermentation of ginseng may contain up to dozens of rare ginsenosides, and the biological effects of these ginsenosides are different, and the biological effects of some rare ginsenosides are even antagonistic to each other. Therefore, such ginsenoside products are more like "panacea", which may have some effects in whitening, anti-anemia, anti-inflammatory, delaying cognitive impairment, and enhancing the body's immunity, but there is no fermented ginseng raw material or product with a particularly clear and outstanding effect in any aspect, especially in anti-fatigue. Summary of the invention

[0006] In view of the above technical problems existing in the prior art, the object of the present invention is to provide a ginseng fermentation product rich in rare ginsenosides with enhanced anti-fatigue effect, and an anti-fatigue composition using the ginseng fermentation product as a raw material, wherein the anti-fatigue composition can resist fatigue by stimulating body functions in a short time, and can relieve fatigue by improving body conditions in the long term.

[0007] The inventors have found through literature research that among the rare ginsenosides, ginsenoside Rh4 is the one that can play an anti-fatigue role, while ginsenosides Rk1 and Rg5 can inhibit nerve excitement and promote sleep, that is, they have the opposite effect on fighting fatigue. Based on this, it can be considered that if the content of ginsenoside Rh4 in ginseng fermentation products can be increased while the content of ginsenosides Rk1 and Rg5 can be reduced, ginseng fermentation products with enhanced anti-fatigue effects can be prepared.

[0008] However, the technical difficulty is that the use of microorganisms (probiotics) to transform natural ginsenosides to produce rare ginsenosides may be related to the transformation path. Among all the rare ginsenosides, ginsenoside Rh4 is difficult to produce, while ginsenosides Rk1 and Rg5 are relatively easy to produce, and often when a certain amount of ginsenoside Rh4 is produced, more ginsenosides Rk1 and Rg5 will be produced. This makes the existing ginseng fermentation products either have a very low content of ginsenoside Rh4, or have contents of ginsenoside Rk1 and Rg5 much higher than that of ginsenoside Rh4, resulting in the ginseng fermentation products being unable to exhibit the anti-fatigue effect of ginsenoside Rh4 as a whole.

[0009] Furthermore, after in-depth research, the inventors found that fermenting ginseng fermentation matrix with a specific composition of Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus casei, and Bifidobacterium adolescens can produce ginseng fermentation products with a high content of ginsenoside Rh4 and a small difference in the content of ginsenosides Rk1 and Rg5, so that the ginseng fermentation products show better anti-fatigue effect.

[0010] Furthermore, by using the above-mentioned ginseng fermentation product as one of the raw materials and adding other anti-fatigue ingredients such as taurine, B vitamins, caffeine, etc., an anti-fatigue composition can be prepared that can resist fatigue by stimulating body functions in a short time and relieve fatigue by improving the body's condition in the long term.

[0011] The technical solution of the present invention includes:

[0012] 1. A ginseng fermentation product obtained by fermenting a ginseng fermentation substrate using a microbial strain, comprising ginsenoside Rh4, ginsenoside Rk1 and ginsenoside Rg5, which satisfies:

[0013] The content of ginsenoside Rh4 is ≥ 3% by weight; and

[0014] Ginsenoside Rh4 content - Ginsenoside Rk1 content - Ginsenoside Rg5 content ≥ - 5% by weight

[0015] The total amount of saponins in the ginseng fermentation product is taken as 100% by weight.

[0016] The ginseng fermentation product may be liquid or solid or a solid-liquid mixture, for example, fermented ginseng liquid or fermented ginseng powder.

[0017] 2. A bacterial composition, which is composed of Lactobacillus plantarum, Lactobacillus casei, Lactobacillus paracasei and Bifidobacterium adolescens; wherein,

[0018] The Lactobacillus plantarum is Lactobacillus plantarum GBFG 61, which is deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration, with a deposit number of GGMCC NO: 31583 and a deposit date of August 14, 2024;

[0019] The Lactobacillus paracasei is Lactobacillus paracasei GBFG 34, which is deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration, with a deposit number of GGMCC NO: 31581 and a deposit date of August 14, 2024.

[0020] 3. The bacterial composition according to item 2, which can be used to ferment ginseng fermentation substrate to produce rare ginsenosides.

[0021] Here, the ginseng fermentation matrix refers to a fermentation matrix containing ginseng fines. The ginseng fines are obtained by reducing ginseng, preferably by physical methods such as extrusion, cutting, grinding, etc. The ginseng fines can be, for example, ginseng powder. The ginseng fermentation matrix can also contain other components commonly contained in the fermentation matrix, such as nitrogen source, carbon source, inorganic salt, pH regulator, etc.

[0022] 4. The bacterial composition according to item 2, wherein the dry weight ratio of Lactobacillus plantarum, Lactobacillus casei, Lactobacillus paracasei, and Bifidobacterium adolescens is: 1:(1-5):(1-5):(1-5).

[0023] 5. The bacterial composition according to item 2, wherein the Lactobacillus casei is Lactobacillus casei BL23.

[0024] 6. The bacterial composition according to item 2, wherein the Bifidobacterium adolescentis (Bifidobacterium adolescens) is Bifidobacterium adolescentis (Bifidobacterium adolescens) BH-J2356.

[0025] 7. A method for preparing a ginseng fermentation product, comprising inoculating a bacterial composition into a ginseng fermentation substrate, and fermenting at 37-42°C, preferably 37°C, for more than 30 hours, preferably 35-60 hours, more preferably 45-55 hours;

[0026] Wherein, the bacterial composition is the bacterial composition described in any one of items 2 to 6.

[0027] 8. The preparation method according to item 7, further comprising sterilizing the ginseng fermentation product.

[0028] 9. The preparation method according to item 7, wherein the ginseng fermentation product is the ginseng fermentation product according to item 1.

[0029] 10. The preparation method according to item 7, wherein the ginseng fermentation product is fermented ginseng liquid or fermented ginseng powder.

[0030] 11. An anti-fatigue composition comprising the ginseng fermentation product according to item 1.

[0031] 12. The anti-fatigue composition according to item 11, further comprising a short-term anti-fatigue ingredient selected from taurine, B vitamins and caffeine.

[0032] The anti-fatigue composition may contain, for example, 20 to 80 parts by weight of the ginseng fermentation product, 10 to 50 parts by weight of taurine, 0.1 to 0.5 parts by weight of B vitamins, and 1 to 20 parts by weight of caffeine; preferably, it contains 30 to 40 parts by weight of the ginseng fermentation product, 20 to 30 parts by weight of taurine, 0.1 to 0.3 parts by weight of B vitamins, and 5 to 15 parts by weight of caffeine.

[0033] The B group vitamins herein may include one or more of vitamin B1, vitamin B2, vitamin B6, and vitamin B12. For example, the B group vitamins may be compounded with vitamin B1, vitamin B2, vitamin B6, and vitamin B12 in a ratio of 1:1:2:80 (by weight).

[0034] The anti-fatigue composition may also be added with one or more of sugars, sugar alcohols, non-sugar and non-sugar alcohol sweeteners, pectin, soluble dietary fiber, salts, other carbohydrates, carbon dioxide or other food additives. Wherein, the sugars include one or more of glucose, sucrose or fructose; the non-sugar and sugar alcohol sweeteners include one or more of stevioside, aspartame, sucralose, monk fruit, glycyrrhizin, betaine or saccharin sodium; the sugar alcohols include one or more of xylitol, erythritol, maltitol, isomalt or sorbitol; the salts include one or more of sodium salt, potassium salt and phosphate; the other carbohydrates include oligosaccharides, starch and / or soluble and insoluble dietary fiber; the other food additives include one or more of flavors, pigments, malic acid, citric acid, sodium citrate, sorbic acid, potassium sorbate, benzoic acid, sodium benzoate, edible gum base, sucrose ester, soybean lecithin and sodium alginate, and the flavors and pigments are artificially synthesized or natural; the caffeine comes from cocoa, coffee, guarana, tea or chocolate. The anti-fatigue composition can be a medicine or food. The food includes milk and dairy products, special dietary foods, convenience foods, baked foods, beverages, etc. When preparing the corresponding products, different types of sweeteners, acidulants, flavoring agents, stabilizers, etc. may be added in accordance with relevant regulations.

[0035] 13. A method for preparing an anti-fatigue composition according to claim 11, comprising:

[0036] The ginseng fermentation product is prepared by the method for preparing the ginseng fermentation product described in any one of items 7 to 10; and

[0037] The ginseng fermentation product is mixed with other ingredients of the anti-fatigue composition to prepare the anti-fatigue composition.

[0038] Effects of the Invention

[0039] In the anti-fatigue composition of the present invention, taurine, B vitamins, caffeine, etc. are classic "refreshing" ingredients, which have the effect of resisting fatigue in the short term; rare ginsenosides can regulate human immunity and blood circulation, and long-term use can improve the body state and relieve fatigue. Therefore, the anti-fatigue composition of the present invention can resist fatigue by stimulating body functions in a short time, and can also relieve fatigue by improving the body state in the long term. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 This is a high performance liquid chromatogram for detecting fermented ginseng powder 2.

[0041] Figure 2 This is a high performance liquid chromatogram for detecting fermented ginseng powder 5.

[0042] Specific embodiments of the invention

[0043] The present invention is described in detail below in conjunction with specific embodiments. In the absence of conflict, the scientific terms in this specification have the meanings commonly understood by those skilled in the art. If there is a conflict, the definitions in this specification shall prevail. Example

[0044] Example 1 Preparation of fermented ginseng powder rich in rare ginsenosides and having enhanced anti-fatigue effect

[0045] The steps include:

[0046] (1) Pretreatment of ginseng fermentation matrix: 120 g ginseng powder, 10 g glucose, 2 g yeast extract powder, 2 g citric acid, 0.05 g manganese sulfate, and 0.1 g magnesium sulfate were added to 856 g water for dissolution, and then sterilized at 121° C. for 20 min before use;

[0047] (2) Preparation of strains: Lactobacillus plantarum GBFG 61, Lactobacillus casei BL23, Lactobacillus paracasei GBFG 34, Bifidobacterium adolescentis BH-J2356, Lactobacillus plantarum Lp90, and Lactobacillus paracasei LC37 were inoculated into 5 mL of MRS broth medium and placed in a constant temperature incubator at 37°C ± 1°C for 24 h to obtain a first-level seed solution. Each bacterial solution was then inoculated into 50 mL of culture medium at a 1% inoculum volume and cultured for 24 h to obtain a second-level seed solution. The activity of each strain was 10 7 cfu / mL to 10 9 cfu / mL. The dry bacteria content in the above-mentioned second-level seed liquid was determined respectively.

[0048] It should be noted that the above-mentioned Lactobacillus plantarum GBFG 61 is a lactobacillus strain that produces β-glucosidase isolated and cultivated from the soil of the ginseng planting base in Fusong County, Baishan City, Jilin Province. The lactobacillus strain was named GBFG 61, which is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee, and the deposit number is CGMCC No.30101. The deposit date is March 22, 2024, and the deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing; Postal code of the deposit institution: 100101.

[0049] The above-mentioned Lactobacillus paracasei GBFG 34 is a lactobacillus strain that produces β-glucosidase isolated and cultivated from the soil of the ginseng planting base in Ji'an City (County) of Tonghua City, Jilin Province. The lactobacillus strain was named GBFG 34, which is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee, and the deposit number is CGMCC No.31581. The deposit date is August 14, 2024, and the deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing; Postal code of the deposit institution: 100101.

[0050] The above-mentioned Lactobacillus casei BL23 was purchased from Beijing Krisbo Biotechnology Co., Ltd.; Bifidobacterium adolescens BH-J2356 was purchased from Shanghai Bohu Biotechnology Co., Ltd.; Lactobacillus plantarum Lp90 and Lactobacillus paracasei LC37 were purchased from Weikang Probiotics (Suzhou) Co., Ltd.

[0051] (3) Fermentation: The second-level seed liquid of Lactobacillus plantarum GBFG 61, Lactobacillus casei BL23, Lactobacillus paracasei GBFG 34, and Bifidobacterium adolescentis BH-J2356 were mixed to 10 g at a dry bacteria weight ratio of 1:1:1:1 (composition 1), 1:2:3:2 (composition 2), 1:3:2:3 (composition 3), and 1:5:5:5 (composition 4), and inoculated aseptically into ginseng fermentation matrix. The fermentation temperature was set to 37° C. and the rotation speed was 50 rpm. The fermentation was carried out for 48 h, and the pH was maintained at 4-5 during the fermentation process.

[0052] As a comparison, the Lactobacillus plantarum Lp90 was used to replace the Lactobacillus plantarum GBFG 61 in the composition 2 (composition 5); and the Lactobacillus paracasei LC37 was used to replace the Lactobacillus paracasei GBFG 34 in the composition 2 (composition 6).

[0053] (4) Centrifugation, drying, and crushing: After the fermentation is completed, sterilization, centrifugation, and drying operations are performed. The sterilization parameters are: 121° C., 20 min; the centrifugation parameters are: 3000-5000 rpm, 10 min; the vacuum degree is controlled to be ≤-0.09 MPa, 60-65° C., and crushed after drying for 48 hours to obtain fermented ginseng powders 1 to 6.

[0054] Detection and analysis: The above-mentioned fermented ginseng powders were dissolved in methanol to prepare 80 mg / mL, and 1 mL of the solution was taken respectively and filtered with a 0.45 μm microporous filter membrane to obtain a clear solution, and the total ginsenoside content and the content of various rare ginsenosides therein were determined, and the difference between the content of ginsenoside Rh4 (forward) related to anti-fatigue and the content of ginsenoside Rk1 (reverse) and Rg5 (reverse) was calculated (weight %, with the total ginsenoside amount as 100 weight %). The results are shown in Table 1.

[0055] The detection method of total ginsenosides was carried out according to the method for determining the content of total ginsenosides in the Pharmacopoeia of the People's Republic of China (2020 edition). 48 H 82 O 18 )count.

[0056] The detection method of rare ginsenosides is carried out according to the method for determining the content of rare ginsenosides in T / CNHFA 001-2021.

[0057] As an example, the HPLC analysis of the fermented ginseng powder 2 obtained by using the above composition 2 is as follows: Figure 1 As shown, the high performance liquid chromatography of the fermented ginseng powder 5 obtained by using the composition 5 is as shown in Figure 2 shown.

[0058] Table 1: Detection of rare ginsenoside content in fermented ginseng powder obtained using different bacterial strain compositions

[0059]

[0060] Difference = Ginsenoside Rh4 content - (Ginsenoside Rk1 content + Ginsenoside Rg5 content)

[0061] As shown in Table 1, the content of ginsenoside Rh4 with anti-fatigue effect in fermented ginseng powders 1 to 4 obtained by using Lactobacillus plantarum GBFG 61 and Lactobacillus paracasei GBFG 34 is more than 3% by weight, and the difference between the content of ginsenoside Rh4 and (the content of ginsenoside Rk1 + the content of ginsenoside Rg5) is more than -5% by weight; on the contrary, the content of ginsenoside Rh4 with anti-fatigue effect in fermented ginseng powders 5 and 6 obtained by using other Lactobacillus plantarum strains and Lactobacillus paracasei strains is less than 3% by weight, and the difference between the content of ginsenoside Rh4 and (the content of ginsenoside Rk1 + the content of ginsenoside Rg5) is less than -5% by weight. It can be expected that fermented ginseng powders 1 to 4 have better anti-fatigue effect than fermented ginseng powders 5 and 6.

[0062] It should also be noted that, in addition to the above-mentioned Lactobacillus plantarum Lp90 and Lactobacillus paracasei LC37 used as comparisons, the inventors also tried a variety of Lactobacillus plantarum and Lactobacillus paracasei strains isolated by themselves, but none of them could achieve the technical effect of "the content of ginsenoside Rh4 is 3 weight % and the difference is greater than -0.5%".

[0063] Example 2 Verification of the Anti-fatigue Effect of Fermented Ginseng Powder

[0064] With reference to Example 6 of Chinese Patent CN 114762529 B, the anti-fatigue effect test of the fermented ginseng powder was slightly modified to confirm that the anti-fatigue effect of the fermented ginseng powder 2 is better than that of the fermented ginseng powder 5. It should be noted that the content of ginsenoside Rh4 in the fermented ginseng powder 2 and the fermented ginseng powder 5 is similar, but only the fermented ginseng powder 2 meets the difference of more than -5% by weight.

[0065] Experimental animals: 60 SPF grade Kunming mice (30 male mice, 30 female mice), weighing 18-22g, were randomly divided into 3 groups, 20 mice / group (10 male mice, 10 female mice in each group), and adaptive feeding was carried out for 7 days. Experimental conditions: SPF animal room, temperature 20-26℃, humidity 40%-70%, light and dark alternation time of 12h / 12h. Dosage grouping and test sample administration time: After the mouse adaptation period, the 3 groups were divided into blank control group, 2 groups of fermented ginseng powder, and 5 groups of fermented ginseng powder; according to the recommended dose of 10 times of human body, the oral administration (250mg / kg) was given for 4 weeks. Experimental method: ① 30min after the last administration of the test sample, the mice with 5% body weight lead at the base of the tail were placed in a swimming box for swimming. The water depth was not less than 20cm, and the water temperature was 25℃±2℃. The time from the start of swimming to death of each group of mice (10 mice in each group) was detected, that is, the weight-bearing swimming time of the mice. ② After the last administration, the mice in each group swam in 30℃ water without weight, and blood was collected from the eye sockets to detect the blood urea nitrogen (BUN), blood lactate (LA), lactate dehydrogenase (LDH), and creatine phosphokinase (CPK) in the serum of each group of mice (10 mice in each group); the liver was separated to detect the content of liver glycogen (LG) in the liver. The test results are shown in Table 2.

[0066] Table 2: Anti-fatigue animal test index detection of fermentation products of different bacterial species

[0067]

[0068] Note: Compared with the blank control group, ***P<0.001, **P<0.01, *P<0.05; compared with composition 3, ###P<0.001, ##P<0.01, #P<0.05

[0069] As can be seen from the above table, compared with the blank control group, the weighted swimming time of the mice in the fermented ginseng powder 2 group was significantly increased, indicating that the ginseng fermentation product of the present invention has the anti-fatigue activity of prolonging the weighted swimming time of mice. Among them, the weighted swimming time of the mice in the fermented ginseng powder 2 group was significantly different from that in the fermented ginseng powder 5 group, which shows that, compared with the fermented ginseng powder 5 group, the fermented ginseng powder 2 group has a stronger anti-fatigue activity in prolonging the weighted swimming time of mice.

[0070] In terms of serum and liver test indicators, the blood lactic acid (LA), urea nitrogen (BUN), and lactate dehydrogenase (LDH) contents of mice in the blank control group, fermented ginseng powder 2, and fermented ginseng powder 5 groups were significantly reduced, and the liver glycogen (LG) content and creatine phosphokinase (CPK) were significantly increased, indicating that both fermented ginseng powder samples have anti-fatigue effects. It is thus proved that the fermented ginseng powder of the embodiment of the present invention may be through reducing the content of LA and BUN, reducing the activity of LDH, and increasing the content of LG and CPK, thereby exerting anti-fatigue activity. Moreover, compared with fermented ginseng powder 5, fermented ginseng powder 2 has significant differences in BUN, LDH, and CPK content.

[0071] Based on the above test results, it can be seen that compared with the comparative fermented ginseng powder 5 (difference less than -0.5 weight %), the fermented ginseng powder 2 of the present invention (difference greater than -0.5 weight %) has significantly stronger anti-fatigue effect.

[0072] Example 3 Preparation of anti-fatigue composition

[0073] The preparation process of the anti-fatigue composition of this embodiment is as follows: add an appropriate amount of water to 234 parts by weight of the aforementioned fermented ginseng powder, 26 parts by weight of taurine, 0.18 parts by weight of B vitamins, 11 parts by weight of caffeine, 30 parts of white sugar, 1 part of citric acid, and 0.5 parts of sodium citrate, and obtain the finished product after dissolution, preparation, filtering, and filling.

[0074] Example 4: Efficacy verification of the anti-fatigue composition

[0075] A total of 20 SPF Kunming mice with a body weight range of 18-22g were selected and randomly divided into a blank control group and an anti-fatigue composition administration group, with half males and half females in each group. All mice were adaptively fed in an SPF animal room for 7 days to ensure that their physiological state was stable. The drug was administered by gavage, and the dosage was 10 times the recommended dosage for humans (250mg / kg). The administration cycle was 4 weeks. The blank control group was given physiological saline, and the anti-fatigue composition administration group was given the anti-fatigue composition prepared in Example 3. 30 minutes after the last administration of the test sample, a lead sheet equivalent to 5% of its body weight was loaded at the base of the mouse's tail, and then it was placed in a swimming box filled with water not less than 20cm deep. The water temperature was maintained at 25℃±2℃. Detection index: The time from the start of swimming to death of each group of mice (10 in each group), that is, the weighted swimming time of the mice, was recorded to evaluate their physical endurance.

[0076] Table 3: Results of weighted swimming test of anti-fatigue composition

[0077]

[0078] Note: Compared with the blank control group, ***P<0.001

[0079] The experimental results showed that the average weighted swimming time of the mice in the blank control group was 7.2±3.52 minutes, while the average weighted swimming time of the mice in the anti-fatigue composition administration group was 20.4±8.63 minutes, which indicates that the anti-fatigue composition of the present invention has a very good anti-fatigue effect.

[0080] It should be noted that, under the premise that it is feasible and does not obviously violate the main purpose of the present invention, any technical feature or combination of technical features described as a component of a technical solution in this specification can also be applied to other technical solutions; and, under the premise that it is feasible and does not obviously violate the main purpose of the present invention, the technical features described as components of different technical solutions can also be combined in any way to form other technical solutions. The present invention also includes technical solutions obtained by combination under the above circumstances, and these technical solutions are equivalent to those recorded in this specification.

[0081] The present invention is described above through specific implementation modes and examples, but those skilled in the art should understand that these are not intended to limit the scope of the present invention, and the scope of the present invention should be determined by the claims.

[0082] Industrial Applicability

[0083] The invention provides an anti-fatigue composition which has both short-term fatigue resistance effect and long-term fatigue relief effect.

Claims

1. An anti-fatigue composition comprising a ginseng fermentation product; wherein: The ginseng fermentation product is obtained by fermenting a ginseng fermentation substrate using a microbial strain, and comprises ginsenoside Rh4, ginsenoside Rk1 and ginsenoside Rg5, which meet the following requirements: The content of ginsenoside Rh4 is ≥ 3% by weight; and Ginsenoside Rh4 content - Ginsenoside Rk1 content - Ginsenoside Rg5 content ≥ - 5% by weight The total amount of saponins in the ginseng fermentation product is taken as 100% by weight. 2 . The anti-fatigue composition according to claim 1 , which is in a liquid or solid form.

3. The anti-fatigue composition according to claim 1, further comprising a short-term anti-fatigue ingredient, wherein the short-term anti-fatigue ingredient is selected from taurine, B vitamins and caffeine.

4. The anti-fatigue composition according to claim 3, comprising 20 to 80 parts by weight of the ginseng fermentation product, 10 to 50 parts by weight of taurine, 0.1 to 0.5 parts by weight of B vitamins and 1 to 20 parts by weight of caffeine; preferably comprising 30 to 40 parts by weight of the ginseng fermentation product, 20 to 30 parts by weight of taurine, 0.1 to 0.3 parts by weight of B vitamins and 5 to 15 parts by weight of caffeine.

5. The anti-fatigue composition according to claim 4, wherein The B vitamins are prepared by compounding vitamin B1, vitamin B2, vitamin B6 and vitamin B12 in a weight ratio of 1:1:2:

80.

6. The method for preparing the anti-fatigue composition according to claim 1, comprising: The preparation of the ginseng fermentation product comprises inoculating the bacterial composition into a ginseng fermentation matrix and fermenting at 37-42° C., preferably 37° C., for more than 30 hours, preferably 35-60 hours, and more preferably 45-55 hours; as well as Mixing the ginseng fermentation product with other ingredients of the anti-fatigue composition to prepare the anti-fatigue composition; The bacterial composition is composed of Lactobacillus plantarum, Lactobacillus casei, Lactobacillus paracasei and Bifidobacterium adolescens; wherein, The plant lactobacillus is plant lactobacillus (Lactobacillus plantarum) GBFG 61, which is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, with a deposit number of GGMCCNO: 31583 and a deposit date of August 14, 2024; The Lactobacillus paracasei is Lactobacillus paracasei GBFG 34, which is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, with a deposit number of GGMCC NO: 31581 and a deposit date of August 14, 2024; The Lactobacillus casei is Lactobacillus casei BL23; and The Bifidobacterium adolescentis is Bifidobacterium adolescens BH-J2356.

7. The preparation method according to claim 6, wherein: The dry weight ratio of Lactobacillus plantarum, Lactobacillus casei, Lactobacillus paracasei and Bifidobacterium adolescentis is 1:(1-5):(1-5):(1-5).

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