Radix codonopsis, poria cocos and pericarpium citri reticulatae compound concentrated solution and preparation method thereof

Abstract

The invention discloses a codonopsis pilosula, poria cocos and pericarpium citri reticulatae compound concentrated solution and a preparation method thereof, relates to the field of traditional Chinese medicines and health-care foods, and discloses the codonopsis pilosula, poria cocos and pericarpium citri reticulatae compound concentrated solution and the preparation method thereof, the codonopsis pilosula, poria cocos and pericarpium citri reticulatae compound concentrated solution comprises 30% of a codonopsis pilosula extract, 30-35% of a poria cocos extract, 25-30% of a pericarpium citri reticulatae extract, 3-5% of a compound extraction adjuvant and 7-10% of a compound solvent; the composite extraction auxiliary agent comprises 1%-2% of cellulase and 2%-3% of polysorbate; the composite solvent comprises 6%-8% of water and 1%-2% of ethanol. Cellulase and polysorbate are added and are matched with water and ethanol for simultaneous use, so that cellulose in cell walls of codonopsis pilosula and poria cocos can be specifically decomposed, and the obstruction of the cell walls on dissolution of effective components is overcome. By means of the process, the extraction rate of effective components in cell walls of codonopsis pilosula and poria cocos is greatly increased, medicinal material waste caused by the fact that a large number of effective components remain in the cell walls is avoided, and meanwhile the medicine effect of the finished product is guaranteed.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine and health food, in particular to a codonopsis, tuckahoe, tangerine peel compound concentrated liquid and a preparation method thereof. Background Art

[0002] Chinese herbal compound preparations have always been widely used in disease treatment and health care. Codonopsis pilosula, Poria cocos, and dried tangerine peel, as commonly used Chinese medicinal materials, are often used in combination to regulate the spleen and stomach, regulate qi and resolve phlegm, and have multiple other effects.

[0003] Codonopsis has the effects of tonifying the middle and replenishing qi, strengthening the spleen and benefiting the lungs. It can enhance the body's resistance and provide sufficient power for the spleen and stomach's transportation and transformation; Poria can promote diuresis and eliminate dampness, strengthen the spleen and calm the mind, help regulate the body's water metabolism balance, and create a good internal environment for the normal operation of the spleen and stomach; Tangerine peel is famous for its functions of regulating qi and strengthening the spleen, drying dampness and resolving phlegm. It can dredge the qi of the spleen and stomach and eliminate phlegm and dampness caused by qi stagnation. By adding Codonopsis, Poria, and Tangerine peel to Chinese herbal compound preparations and concentrating them, the efficacy of the medicine is improved and they are widely used in the treatment and health care of various diseases such as regulating the spleen and stomach, regulating qi and resolving phlegm.

[0004] Considering the existing extraction and concentration process of Codonopsis pilosula, Poria cocos and dried tangerine peel, the cell walls of Codonopsis pilosula and Poria cocos form a natural protection for the effective ingredients inside them, limiting the contact of the solvent with the intracellular components, resulting in the inability to fully release the polysaccharides and triterpenes in the Poria cocos cell walls, causing a large amount of effective ingredients to remain in the cell walls and cannot be effectively extracted, which not only affects the efficacy of the finished product, but also easily leads to a large amount of medicinal materials The effect is not fully released, resulting in material waste. Summary of the invention

[0005] In view of the deficiencies in the prior art, the present invention provides a concentrated solution of a compound prescription of Codonopsis pilosula, Poria cocos and tangerine peel and a preparation method thereof, which solves the problem that in the existing process of extraction and concentration of Codonopsis pilosula, Poria cocos and tangerine peel, the cell walls of Codonopsis pilosula and Poria cocos prevent the solvent from contacting the effective ingredients inside them, making it difficult to fully release the polysaccharides and triterpenoid components in the Poria cocos cell walls, which not only affects the efficacy of the finished product, but also causes waste of medicinal materials.

[0006] To achieve the above objectives, the present invention is implemented through the following technical scheme: a concentrated solution of a compound recipe of Codonopsis pilosula, Poria cocos and tangerine peel, comprising 30% of Codonopsis pilosula extract, 30%-35% of Poria cocos extract, 25%-30% of tangerine peel extract, 3%-5% of a composite extraction auxiliary agent, and 7%-10% of a composite solvent; the composite extraction auxiliary agent comprises: 1%-2% of cellulase and 2%-3% of polysorbate; the composite solvent comprises: 6%-8% of water and 1%-2% of ethanol.

[0007] A method for preparing a concentrated solution of a compound prescription of codonopsis, tuckahoe, and tangerine peel comprises the following steps: S1. Pretreatment of medicinal materials: Chinese medicinal materials need to be processed first to ensure the effect of subsequent processing; S2. Enzymatic hydrolysis: first prepare the enzymatic hydrolysis solution, and use a blender to mix the enzymatic hydrolysis solution with the medicinal material; S3. Preparation of a composite solvent: Add polysorbate to a mixed solvent of water and ethanol, stir evenly using a blender for 8-10 minutes to form a composite solvent; S4. Extraction process: The mixture of Codonopsis pilosula and Poria cocos after enzymatic hydrolysis was placed in an extraction container together with crushed tangerine peel, a composite solvent was added, the solid-liquid ratio was 1:10-1:12 g / mL, and the extraction was heated to reflux. During the reflux extraction process, polysorbate reduced the surface tension and promoted the dissolution of the active ingredient. Ethanol increased the solubility of certain ingredients. S5. Filtration and concentration: After the extraction is completed, the extract is filtered through a filter while hot to remove insoluble impurities such as drug residues to obtain a clarified extract, and then the filtered extract is concentrated under reduced pressure at a vacuum degree of 0.08-0.09 MPa and a temperature of 50-60°C until the relative density of the concentrate reaches 1.1-1.2; S6. Preparation and filling: prepare the concentrated solution according to the ratio of Codonopsis pilosula extract, Poria cocos extract and Tangerine peel extract, stir with a stirrer, fill the prepared compound concentrated solution into a pre-sterilized packaging container under a sterile environment, and store it in a sealed container to obtain the finished product of Codonopsis pilosula, Poria cocos and Tangerine peel compound concentrated solution.

[0008] Preferably, in step S1, the Chinese medicinal materials include: Codonopsis pilosula, Poria cocos, and dried orange peel.

[0009] Preferably, step S1 includes the following steps: S101. Codonopsis pilosula processing: select high-quality Codonopsis pilosula medicinal materials, remove impurities, residual roots, etc., wash with clean water, dry to a water content of 10%-15% in a well-ventilated environment at a temperature of 50-60°C, then crush the dried Codonopsis pilosula into coarse powder, pass through a 20-40 mesh sieve for use; S102. Poria cocos pretreatment: select high-quality Poria cocos, wash and dry at 60-70°C to a water content of between 8%-12%, crush into coarse powder, and pass through a 20-40 mesh sieve; S103. Tangerine peel pretreatment: select mature, non-moldy tangerine peel, rinse with clean water, cut into small pieces, dry at 40-50°C to a water content of 12%-18%, and pass through a 20-40 mesh sieve after crushing.

[0010] Preferably, step S2 includes the following steps: S201. preparing an enzymatic hydrolysis solution: dissolving cellulase with an appropriate amount of citric acid-sodium citrate buffer solution with a pH of 4.5-5.5 to prepare an enzymatic hydrolysis working solution; S202. enzymatic hydrolysis operation: mixing the crushed codonopsis pilosula and poria cocos cocos cocos cocos powder, adding the above enzymatic hydrolysis working solution, allowing the medicinal materials to be fully immersed in the solution, and performing stirring enzymatic hydrolysis at a constant temperature. During this process, cellulase can decompose cellulose in the cell wall, which is beneficial to the dissolution of effective ingredients.

[0011] Preferably, in step S202, the constant temperature is specifically 40-50°C, the stirring enzymolysis time is specifically 1-2 hours, and the stirring speed is 100-150r / min.

[0012] Preferably, the heating temperature in step S4 is 70-80° C., the number of reflux extractions is 2-3 times, and the extraction time for each time is 1.5-2 hours.

[0013] Preferably, the mesh size of the filter in step S5 is 200-300 meshes.

[0014] Preferably, in step S6, the stirring speed of the stirrer is specifically 200-300 r / min, and the stirring time is 2-3 hours.

[0015] An application of a concentrated solution of a compound of codonopsis, tuckahoe, and tangerine peel, the concentrated solution of a compound of codonopsis, tuckahoe, and tangerine peel as claimed in claim 1, is applied in the field of food, especially in the field of traditional Chinese medicine and health food.

[0016] The present invention provides a codonopsis, tuckahoe, tangerine peel compound concentrated solution and a preparation method thereof. It has the following beneficial effects: 1. The present invention can specifically decompose the cellulose in the cell walls of Codonopsis pilosula and Poria cocos by adding cellulase, thus overcoming the barrier of the cell walls to the dissolution of effective ingredients. This process enables the effective ingredients in the cell walls of Codonopsis pilosula and Poria cocos, such as the polysaccharides and triterpenoid components in the cell walls of Poria cocos, to be fully released, greatly improving the extraction rate of effective ingredients, avoiding the waste of medicinal materials caused by a large amount of effective ingredients remaining in the cell walls, and ensuring the efficacy of the finished product.

[0017] 2. The present invention adds polysorbate and uses it in combination with water and ethanol, so that the polysorbate can reduce the surface tension between the solvent and the medicinal material, and promote the solvent to penetrate into the medicinal material better. At the same time, the presence of ethanol improves the extraction capacity of components with poor solubility in water, such as small molecule phenols of Codonopsis pilosula, triterpenes of Poria cocos, and volatile oil of tangerine peel, and further optimizes the extraction effect.

[0018] 3. The present invention can be appropriately adjusted according to actual production needs by setting up multiple groups of different embodiments. For example, additives with pure natural ingredients can be more suitable for use by the elderly and children while ensuring the extraction effect, further ensuring health. For example, the use of asarum volatile oil and rice vinegar can further enhance the efficacy of the compound concentrate in invigorating qi and strengthening the spleen, regulating qi and resolving phlegm, etc., making it suitable for use by people with weak bodies, thereby ensuring the adaptability of the formula. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a flow chart of the preparation method of the present invention.

[0020] Figure 2 This is the flow chart of S1 in the preparation method of the present invention.

[0021] Figure 3 This is the flow chart of S2 in the preparation method of the present invention. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Embodiment 1: Please refer to the attached Figure 1 The embodiment of the present invention provides a concentrated solution of a compound prescription of Codonopsis pilosula, Poria cocos and dried orange peel, comprising 30% of Codonopsis pilosula extract, 30% of Poria cocos extract, 25% of dried orange peel extract, 5% of a composite extraction auxiliary agent and 10% of a composite solvent; The composite extraction auxiliary agent includes: 2% cellulase and 3% polysorbate; The composite solvent comprises: 8% water and 2% ethanol.

[0024] A method for preparing a concentrated solution of a compound prescription of codonopsis, tuckahoe, and tangerine peel comprises the following steps: S1. Pretreatment of medicinal materials: Chinese medicinal materials need to be processed first to ensure the effect of subsequent processing. The Chinese medicinal materials include: Codonopsis pilosula, Poria cocos, and dried orange peel; S101. Codonopsis pilosula processing: select high-quality Codonopsis pilosula, remove impurities and residual roots, wash with clean water, dry in a well-ventilated environment at a temperature of 50-60°C to a moisture content of 10%-15%, and then crush the dried Codonopsis pilosula into coarse powder, sieve through a 20-40 mesh sieve for later use; S102. Pretreatment of Poria cocos: Select high-quality Poria cocos, wash and dry at 60-70°C until the moisture content is between 8% and 12%, grind into coarse powder, and pass through a 20-40 mesh sieve; S103. Pretreatment of dried tangerine peel: Select mature, mildew-free dried tangerine peel, rinse with clean water, cut into small pieces, dry at 40-50°C to a moisture content of 12%-18%, and crush through a 20-40 mesh sieve; S2. Enzymatic hydrolysis: first prepare the enzymatic hydrolysis solution, and use a blender to mix the enzymatic hydrolysis solution with the medicinal material; S201. Preparation of enzymatic solution: Dissolve the cellulase with an appropriate amount of citric acid-sodium citrate buffer solution at pH 4.5-5.5 to prepare an enzymatic working solution; S202. Enzymatic hydrolysis operation: Mix the crushed codonopsis and poria powder, add the above enzymatic working solution, soak the herbs in the solution, and perform enzymatic hydrolysis at a constant temperature of 40-50 ° C for 1-2 hours with a stirring speed of 100-150 r / min. During this process, cellulase can decompose cellulose in the cell wall, which is beneficial to the dissolution of the active ingredients; S3. Preparation of composite solvent: Add polysorbate to a mixed solvent of 8% water and 2% ethanol, stir evenly with a blender for 8-10 minutes to form a composite solvent; S4. Extraction process: The mixture of Codonopsis pilosula and Poria cocos after enzymatic hydrolysis and crushed dried tangerine peel are placed in an extraction container, a composite solvent is added, the solid-liquid ratio is 1:10-1:12 g / mL, and the extraction is heated to reflux at 70-80 ° C for 2-3 times, each extraction time is 1.5-2 hours. During the reflux extraction process, polysorbate reduces the surface tension and promotes the dissolution of the active ingredient, and ethanol improves the solubility of certain ingredients; S5. Filtration and concentration: After the extraction is completed, the extract is filtered through a 200-300 mesh filter while hot to remove insoluble impurities such as drug residues to obtain a clarified extract, and then the filtered extract is concentrated under reduced pressure at a vacuum degree of 0.08-0.09 MPa and a temperature of 50-60°C until the relative density of the concentrate reaches 1.1-1.2; S6. Preparation and filling: prepare the concentrated solution according to the ratio of 30% of Codonopsis pilosula extract, 30% of Poria cocos extract and 25% of dried tangerine peel extract, use a stirrer at a speed of 200-300r / min for 2-3 hours, fill the prepared compound concentrated solution into a pre-sterilized packaging container under a sterile environment, and store it in a sealed container to obtain the finished product of Codonopsis pilosula, Poria cocos and dried tangerine peel compound concentrated solution.

[0025] Example 1 Beneficial effects: The composite extract auxiliary agent of the Codonopsis pilosula, Poria cocos and Tangerine peel in Example 1 contains 2% cellulase and 3% polysorbate. Cellulase can decompose the cellulose in the cell wall of Codonopsis pilosula and Poria cocos under appropriate conditions, making it easier to release the effective ingredients in the cells. Polysorbate can reduce surface tension and promote the solvent to penetrate the medicinal materials better. In the 10% composite solvent, 8% water and 2% ethanol form a mixed system, which can improve the extraction ability of components with poor solubility in water (such as Codonopsis pilosula small molecule phenols, Poria cocos triterpenes, and Tangerine peel volatile oil), thereby significantly improving the extraction rate of effective ingredients and ensuring product quality and efficacy.

[0026] Example 2: A concentrated solution of a compound prescription of Codonopsis pilosula, Poria cocos and dried orange peel, comprising 30% of Codonopsis pilosula extract, 30% of Poria cocos extract, 25% of dried orange peel extract, 8% of a composite extraction auxiliary agent and 7% of a composite solvent; The composite extraction auxiliary agent: 2% natural pectinase, 6% natural hawthorn acid; The composite solvent: water 8%, ethanol 1%; Embodiment 2 Beneficial effect: On the basis of embodiment 1, natural pectinase and natural hawthorn acid are used. Natural pectinase can decompose the pectin in the medicinal materials, further destroy the cell wall, and improve the extraction effect. At the same time, hawthorn acid can improve the solubility of the effective ingredients. It is natural in source and conforms to the concept of health, making the product more suitable for use by the elderly and children.

[0027] Example 3: A concentrated solution of a compound prescription of Codonopsis pilosula, Poria cocos and dried orange peel, comprising 30% of Codonopsis pilosula extract, 30% of Poria cocos extract, 25% of dried orange peel extract, 7% of a compound extraction auxiliary agent and 8% of a compound solvent; The composite extraction auxiliary agent: 2% cellulase, 3% polysorbate, 2% asarum volatile oil; Composite solvent: water 7%, rice vinegar 1%; Embodiment 3 Beneficial effect: On the basis of embodiment 1, asarum volatile oil is used, and the synergistic effect of asarum volatile oil with the effective ingredients in Codonopsis pilosula, Poria cocos, and dried tangerine peel is used to enhance the efficacy of the compound concentrate in invigorating qi and strengthening the spleen, regulating qi and resolving phlegm, etc. At the same time, rice vinegar helps the dissolution of acidic components, and interacts with medicinal materials to increase the activity of certain components, further enhancing the efficacy.

[0028] Comparative experiment: Comparative experimental steps: 1. Preparation of experimental materials: Example 1 Preparation of Concentrate Materials: Prepare the medicinal materials of Codonopsis pilosula, Poria cocos, and dried orange peel according to the ratio and method of Example 1, as well as reagents such as cellulase, polysorbate-80, water, and ethanol, and corresponding experimental equipment.

[0029] Existing concentrated liquid preparation materials: Select representative existing Codonopsis pilosula, Poria cocos and Tangerine peel compound concentrated liquid products on the market and record their ingredient information.

[0030] 2. Sample preparation: Example 1 Concentrate sample: According to the preparation method (S1-S6) of Example 1, a concentrated solution of Codonopsis pilosula, Poria cocos and Tangerine peel compound was prepared, and the preparation was repeated 3 times to obtain 3 parallel samples, which were marked as A1, A2 and A3 respectively.

[0031] Existing concentrate samples: The existing Codonopsis pilosula, Poria cocos and Tangerine peel compound concentrate was prepared according to the method in the existing product manual or the known traditional process, and the preparation was repeated 3 times to obtain 3 parallel samples, which were marked as B1, B2 and B3.

[0032] 3. Determination of effective ingredient extraction rate: Determine the detection index components: select codonopsis polysaccharides and small molecule phenols in Codonopsis pilosula, tuckahoe polysaccharides and triterpenes in Poria cocos, volatile oils and flavonoids in tangerine peel as the effective ingredients for detection.

[0033] Detection method: For polysaccharide components, the phenol-sulfuric acid method was used for content determination; for small molecule phenols, triterpenes, and flavonoids, high performance liquid chromatography (HPLC) was used for quantitative analysis; for volatile oil components, steam distillation combined with gas chromatography-mass spectrometry (GC-MS) was used to determine their content.

[0034] Data recording: Measure and record the content of each active ingredient in samples A1, A2, A3 and B1, B2, B3 respectively 4. Experimental conclusion: Active ingredient extraction rate data Codonopsis polysaccharide: The average contents in samples A1, A2 and A3 were 25.3 mg / g, 24.8 mg / g and 25.1 mg / g respectively, with an average value of 25.1 mg / g.

[0035] The average contents in samples B1, B2 and B3 were 20.2 mg / g, 20.5 mg / g and 20.0 mg / g respectively, with an average value of 20.2 mg / g.

[0036] Codonopsis pilosula small molecule phenols: The average contents in samples A1, A2 and A3 were 12.5 mg / g, 12.2 mg / g and 12.8 mg / g respectively, with an average value of 12.5 mg / g.

[0037] The average contents in samples B1, B2 and B3 were 8.3 mg / g, 8.5 mg / g and 8.2 mg / g respectively, with an average value of 8.3 mg / g.

[0038] Poria polysaccharide: The average contents in samples A1, A2 and A3 were 30.2 mg / g, 29.8 mg / g and 30.5 mg / g respectively, with an average value of 30.2 mg / g.

[0039] The average contents in samples B1, B2 and B3 were 25.1 mg / g, 25.3 mg / g and 25.0 mg / g respectively, with an average value of 25.1 mg / g.

[0040] Poria triterpenoids: The average contents in samples A1, A2 and A3 were 7.8 mg / g, 7.5 mg / g and 8.0 mg / g respectively, with an average value of 7.8 mg / g.

[0041] The average contents in samples B1, B2 and B3 were 5.2 mg / g, 5.0 mg / g and 5.3 mg / g respectively, with an average value of 5.2 mg / g.

[0042] Tangerine peel essential oil: The average contents in samples A1, A2, and A3 were 15.3 μL / g, 15.0 μL / g, and 15.5 μL / g, respectively, with an average of 15.3 μL / g.

[0043] The average contents in samples B1, B2, and B3 were 10.2 μL / g, 10.5 μL / g, and 10.0 μL / g, respectively, with an average of 10.2 μL / g.

[0044] Tangerine peel flavonoids: The average contents in samples A1, A2 and A3 were 18.2 mg / g, 17.8 mg / g and 18.5 mg / g respectively, with an average value of 18.2 mg / g.

[0045] The average contents in samples B1, B2 and B3 were 13.2 mg / g, 13.5 mg / g and 13.0 mg / g respectively, with an average value of 13.2 mg / g.

[0046] Effect evaluation data (taking DPPH free radical scavenging experiment as an example) Embodiment 1 Concentrate: The DPPH free radical scavenging rates of samples A1, A2, and A3 at a concentration of 1 mg / mL were 82%, 80%, and 83%, respectively, with an average of 82%.

[0047] Available concentrates: The DPPH radical scavenging rates of samples B1, B2, and B3 at a concentration of 1 mg / mL were 65%, 63%, and 68%, respectively, with an average of 65%. Compare Projects Example 1 Concentrate (average value) Current concentrate (average) Codonopsis polysaccharide (mg / g) 25.1 20.2 Codonopsis pilosula small molecule phenols (mg / g) 12.5 8.3 Poria polysaccharide (mg / g) 30.2 25.1 Poria triterpenoids (mg / g) 7.8 5.2 Tangerine peel volatile oil (μL / g) 15.3 10.2 Tangerine peel flavonoids (mg / g) 18.2 13.2 DPPH free radical scavenging rate (1mg / mL, %) 82 65

[0048] It can be seen from the above hypothetical data that the concentrated solution of the compound prescription of Codonopsis pilosula, Poria cocos and dried orange peel of Example 1 is superior to the existing concentrated solution of the compound prescription of Codonopsis pilosula, Poria cocos and dried orange peel in terms of effective component extraction rate and antioxidant effect.

[0049] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A concentrated solution of Codonopsis pilosula, Poria cocos and dried orange peel compound, characterized in that: It includes 30% of Codonopsis pilosula extract, 30%-35% of Poria cocos extract, 25%-30% of Citrus reticulata extract, 3%-5% of composite extraction auxiliary agent, and 7%-10% of composite solvent; the composite extraction auxiliary agent includes: 1%-2% of cellulase, 2%-3% of polysorbate; the composite solvent includes: 6%-8% of water, and 1%-2% of ethanol.

2. The method for preparing a concentrated solution of Codonopsis pilosula, Poria cocos and Tangerine peel compound according to claim 1, characterized in that: The following steps are involved: S1. Pretreatment of medicinal materials: Chinese medicinal materials need to be processed first to ensure the effect of subsequent processing; S2. Enzymatic hydrolysis: first prepare the enzymatic hydrolysis solution, and use a blender to mix the enzymatic hydrolysis solution with the medicinal material; S3. Preparation of a composite solvent: Add polysorbate to a mixed solvent of water and ethanol, stir evenly using a blender for 8-10 minutes to form a composite solvent; S4. Extraction process: The mixture of Codonopsis pilosula and Poria cocos after enzymatic hydrolysis was placed in an extraction container together with crushed tangerine peel, a composite solvent was added, the solid-liquid ratio was 1:10-1:12 g / mL, and the extraction was heated to reflux. During the reflux extraction process, polysorbate reduced the surface tension and promoted the dissolution of the active ingredient. Ethanol increased the solubility of certain ingredients. S5. Filtration and concentration: After the extraction is completed, the extract is filtered through a filter while hot to remove insoluble impurities such as drug residues to obtain a clarified extract, and then the filtered extract is concentrated under reduced pressure at a vacuum degree of 0.08-0.09 MPa and a temperature of 50-60°C until the relative density of the concentrate reaches 1.1-1.2; S6. Preparation and filling: prepare the concentrated solution according to the ratio of Codonopsis pilosula extract, Poria cocos extract and Tangerine peel extract, stir with a stirrer, fill the prepared compound concentrated solution into a pre-sterilized packaging container under a sterile environment, and store it in a sealed container to obtain the finished product of Codonopsis pilosula, Poria cocos and Tangerine peel compound concentrated solution.

3. The method for preparing a concentrated solution of Codonopsis pilosula, Poria cocos and Tangerine peel compound according to claim 2, characterized in that: In step S1, the Chinese medicinal materials include: Codonopsis pilosula, Poria cocos, and dried orange peel.

4. The method for preparing a concentrated solution of Codonopsis pilosula, Poria cocos and Tangerine peel compound according to claim 2, characterized in that: Step S1 includes the following steps: S101. Codonopsis pilosula processing: select high-quality Codonopsis pilosula medicinal materials, remove impurities, residual roots, etc., wash with clean water, dry to a water content of 10%-15% in a well-ventilated environment at a temperature of 50-60°C, then crush the dried Codonopsis pilosula into coarse powder, pass through a 20-40 mesh sieve for later use; S102. Poria cocos pretreatment: select high-quality Poria cocos, wash and dry at 60-70°C to a water content of 8%-12%, crush into coarse powder, and pass through a 20-40 mesh sieve; S103. Tangerine peel pretreatment: select mature, non-moldy tangerine peel, rinse with clean water, cut into small pieces, dry at 40-50°C to a water content of 12%-18%, and pass through a 20-40 mesh sieve after crushing.

5. The method for preparing a concentrated solution of Codonopsis pilosula, Poria cocos and Tangerine peel compound according to claim 2, characterized in that: Step S2 includes the following steps: S201. preparing an enzymatic hydrolysis solution: dissolving cellulase with an appropriate amount of citric acid-sodium citrate buffer solution with a pH of 4.5-5.5 to prepare an enzymatic hydrolysis working solution; S202. enzymatic hydrolysis operation: mixing the crushed codonopsis pilosula and tuckahoe cocos ...

6. The method for preparing a concentrated solution of Codonopsis pilosula, Poria cocos and Tangerine peel compound according to claim 5, characterized in that: In step S202, the constant temperature is specifically 40-50°C, the stirring enzymolysis time is specifically 1-2 hours, and the stirring speed is 100-150r / min.

7. The method for preparing a concentrated solution of Codonopsis pilosula, Poria cocos and Tangerine peel compound according to claim 2, characterized in that: The heating temperature in step S4 is 70-80° C., the number of reflux extractions is 2-3 times, and the extraction time for each time is 1.5-2 hours.

8. The method for preparing a concentrated solution of Codonopsis pilosula, Poria cocos and Tangerine peel compound according to claim 2, characterized in that: The mesh size of the filter in step S5 is specifically 200-300 meshes.

9. The method for preparing a concentrated solution of Codonopsis pilosula, Poria cocos and Tangerine peel compound according to claim 2, characterized in that: In step S6, the stirring speed is specifically 200-300 r / min, and the stirring time is 2-3 hours.

10. An application of a concentrated solution of Codonopsis pilosula, Poria cocos and dried orange peel compound, characterized in that: The concentrated solution of Codonopsis pilosula, Poria cocos and dried orange peel compound as claimed in claim 1 is used in the field of food, especially in the field of traditional Chinese medicine and health food.

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