Cream for removing cow nipple keratinized tissues and preparation method thereof

Abstract

The invention relates to a cream for removing cow nipple keratinized tissues. The cream is prepared from the following raw materials by weight: 1-2 g of a bletilla striata extract, 7.5 g of jojoba oil, 1.5 g of an emulsifier, 0.5 g of glyceryl monostearate, 1 g of PCA sodium, 4.5 g of glycerin, 0.5 g of vitamin E, 3 g of salicylic acid, 1 g of a moutan bark extracting solution, and the balance of distilled water to 50 g. By adding the bletilla striata extract, the glycerol, the salicylic acid and the jojoba oil into the exfoliating traditional Chinese medicine cream, the cream prepared according to the formula has exfoliating, skin moistening and moisturizing effects, and has a better treatment condition on nipple skin of dairy cows.

Description

Technical Field

[0001] The invention belongs to the field of animal medicine and relates to an ointment for removing keratinized tissue of a cow nipple and a preparation method thereof. Background Art

[0002] The teat duct is the first physical / chemical barrier for mastitis pathogens to invade the cow's udder. The sphincter around the teat duct contracts to close the teat duct tightly, preventing pathogens from invading the mammary gland from the teat orifice. Good teat skin health is critical to preventing bacteria from proliferating on the mammary skin. Changes in teat tissue caused by milking, teat duct integrity, and teat skin flexibility can affect the invasion of pathogens into the mammary tissue, thereby affecting the risk of mastitis infection. Day-to-day milking operations can cause changes in the skin tissue at the end of the teat of dairy cows, such as the appearance of a keratin ring around the teat orifice. Factors such as the teat end characteristics, teat position, teat length, milk production, lactation stage, and parity of dairy cows are all related to the formation of a keratin ring at the end of the teat.

[0003] At present, the keratin ring at the end of the teat of dairy cows is mostly removed by medicated bathing liquid, but improper selection or use of medicated bathing liquid may cause dry skin at the base of the teat, causing skin inflammation and skin damage, and the cost of medicated bathing liquid treatment is relatively high. Therefore, it is necessary to invent a cream for removing keratinized tissue of the teat of dairy cows to address the above problems. This project is funded by the Central Guidance Local Science and Technology Development Fund Project (Achievement Transfer and Transformation Direction) (No. ZY23CG32) and the Heilongjiang Province "Unveiling the List and Leading the Leader" Science and Technology Research Project (No. 2023ZXJ02B03). Summary of the invention

[0004] The first purpose of the present invention is to provide an ointment for removing keratinized tissue of the teat of a dairy cow, so as to overcome the problem that the keratin ring at the end of the teat of a dairy cow is currently mostly removed by bathing liquid, and improper selection or use of the bathing liquid may cause dryness of the skin at the base of the teat, causing skin inflammation and skin damage.

[0005] The second object of the present invention is to provide a method for preparing the above-mentioned cream for removing keratinized tissue of cow nipples.

[0006] The third object of the present invention is to provide use of the above cream.

[0007] The present invention is achieved through the following technical solutions: 1. A cream for removing keratinized tissue of cow nipples, made from the following raw materials in weight ratio: 1-2g of bletilla striata extract, 7.5g of jojoba oil, 1.5g of emulsifier, 0.5g of glyceryl monostearate, 1g of sodium PCA, 4.5g of glycerol, 0.5g of vitamin E, 3g of salicylic acid, 1g of peony bark extract, and the remaining distilled water is added to 50g.

[0008] Further, it is made from the following raw materials in the following weight ratio: 1.5g of Bletilla striata extract, 7.5g of jojoba oil, 1.5g of emulsifier, 0.5g of glyceryl monostearate, 1g of sodium PCA, 4.5g of glycerol, 0.5g of vitamin E, 3g of salicylic acid, 1g of Paeonia suffruticosa root extract, and the remaining distilled water is added to 50g.

[0009] 2. According to the above-mentioned method for preparing an ointment for removing keratinized tissue of a cow's nipple, the specific steps are as follows: S1: preparing the oil phase drug: heating jojoba oil, glyceryl monostearate and vitamin E until melted and then adding an emulsifier; S2: preparing aqueous phase medicine: heating the bletilla striata extract, salicylic acid, sodium PCA, glycerin and water until they are melted, and then adding the peony bark extract; S3: Mix the oil phase medicine and the water phase medicine and stir until they are uniform to obtain a finished cream.

[0010] 3. Application of the above-mentioned cream in removing keratinized tissue from the nipples of dairy cows.

[0011] Positive effects of adopting the above technical scheme: the experimental procedure of the present invention is more convenient and quick, and does not require refined details and cumbersome operations. By adding bletilla striata extract, glycerin, salicylic acid and jojoba oil to the exfoliating Chinese medicine cream, the bletilla striata extract has good local astringent hemostasis and swelling and muscle-regenerating effects, glycerin has moisturizing, relieves dry skin, protects the skin from external environmental stimulation, and promotes the self-repair of damaged cells, salicylic acid can dissolve the constituent substances between cuticles and cause the stratum corneum to fall off, so it can remove the stratum corneum that is accumulated too thick, and jojoba oil has the effect of moisturizing the skin, relieving dryness, and enhancing the elasticity and moisturizing ability of the skin. Therefore, the cream prepared by the formula has the effects of exfoliating, emollient and moisturizing, and has a good treatment effect on the nipple skin of dairy cows. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 is a flow chart of the present invention; Figure 2 The figure is the appearance and properties of the exfoliating cream, where A is the prepared cream, B is the ductility of the cream, and C is the microscope observation (400×) figure; Figure 3 The stability test results are shown in Figure 1, where A is the centrifugation test group, B is the cold-resistant group (-20°C), and C is the heat-resistant group (45°C); Figure 4 A graph showing the results of a comparative test of applying cream to cow teats. DETAILED DESCRIPTION

[0013] The technical solution of the present invention is further described below in conjunction with specific embodiments and test examples, but they should not be construed as limiting the present invention. Example

[0014] A method for preparing an ointment for removing keratinized tissue of a cow nipple is prepared from the following raw materials in a weight ratio: 1g of bletilla striata extract, 7.5g of jojoba oil, 1.5g of an emulsifier, 0.5g of glyceryl monostearate, 1g of sodium PCA, 4.5g of glycerol, 0.5g of vitamin E, 3g of salicylic acid, 1g of peony bark extract, and the remaining distilled water is added to 50g.

[0015] The specific steps are as follows: S1: preparing the oil phase drug: heating jojoba oil, glyceryl monostearate and vitamin E until melted and then adding an emulsifier; S2: preparing aqueous phase medicine: heating the bletilla striata extract, salicylic acid, sodium PCA, glycerin and water until they are melted, and then adding the peony bark extract; S3: Mix the oil phase medicine and the water phase medicine and stir until they are uniform to form an ointment.

[0016] Preparation process see Figure 1 shown. Example

[0017] The invention discloses a method for preparing an ointment for removing keratinized tissue of a cow nipple. The ointment is prepared from the following raw materials in a weight ratio: 1.5 g of bletilla striata extract, 7.5 g of jojoba oil, 1.5 g of an emulsifier, 0.5 g of glyceryl monostearate, 1 g of sodium PCA, 4.5 g of glycerol, 0.5 g of vitamin E, 3 g of salicylic acid, 1 g of a peony bark extract, and the remaining distilled water is added to 50 g.

[0018] The specific steps are as follows: S1: preparing the oil phase drug: heating jojoba oil, glyceryl monostearate and vitamin E until melted and then adding an emulsifier; S2: preparing aqueous phase medicine: heating the bletilla striata extract, salicylic acid, sodium PCA, glycerin and water until they are melted, and then adding the peony bark extract; S3: Mix the oil phase medicine and the water phase medicine and stir until they are uniform to form an ointment. Example

[0019] A method for preparing an ointment for removing keratinized tissue of a cow nipple is provided. The ointment is prepared from the following raw materials in a weight ratio: 2 g of bletilla striata extract, 7.5 g of jojoba oil, 1.5 g of an emulsifier, 0.5 g of glyceryl monostearate, 1 g of sodium PCA, 4.5 g of glycerol, 0.5 g of vitamin E, 3 g of salicylic acid, 1 g of a peony bark extract, and the remaining distilled water is added to 50 g.

[0020] The specific steps are as follows: S1: preparing the oil phase drug: heating jojoba oil, glyceryl monostearate and vitamin E until melted and then adding an emulsifier; S2: preparing aqueous phase medicine: heating the bletilla striata extract, salicylic acid, sodium PCA, glycerin and water until they are melted, and then adding the peony bark extract; S3: Mix the oil phase medicine and the water phase medicine and stir until they are uniform to form an ointment.

[0021] The properties of the cream prepared in Example 2 were tested.

[0022] First, the prepared cream for removing keratinized tissue of the cow's nipples was observed; secondly, the prepared cream for removing keratinized tissue of the cow's nipples was applied to a glass slide to observe the ductility and uniform texture; then, the prepared cream for removing keratinized tissue of the cow's nipples was observed under an optical microscope (400×), and there were no large amounts of bubbles. Figure 2 shown.

[0023] The stability of the cream prepared in Example 3 was tested.

[0024] The specific steps are as follows: S1: The prepared cream for removing keratinized tissue of dairy cow nipples was observed at a centrifugal speed of 3000 rpm / min, and no demulsification or demulsification was observed; S2: The prepared cream for removing keratinized tissue of dairy cow nipples was placed in a -20°C refrigerator overnight, and no freezing stratification occurred; S3: The prepared cream for removing keratinized tissue of cow nipples was placed in an oven at 45° C. overnight, and no hydrolysis or stratification occurred.

[0025] Results Figure 3 shown.

[0026] The cream formulations in Examples 1-3 were subjected to a scoring test.

[0027] The prepared cream for removing keratinized tissue of dairy cow nipples was evaluated according to the inspection indexes and scoring standards, and scored as follows.

[0028] Evaluation indicators: appearance, stability, and particle size. Scoring criteria: The full score is 100 points, of which appearance accounts for 60%, stability accounts for 20%, and particle size accounts for 20%.

[0029] Evaluation indicators of cream appearance: gloss (inspecting the feeling of use), whiteness (inspecting the color comfort of use), fineness (inspecting the skin feel experience), consistency and spreadability (inspecting the effect of drug application). Take each prescription cream for visual observation and apply it on the back of the hand. Scoring rules for the five indicators: The comprehensive score is 100 points, of which gloss accounts for 20%, whiteness accounts for 20%, fineness accounts for 20%, consistency accounts for 20%, and spreadability accounts for 20%. Among them, gloss and consistency: +++ means very good (10 points), ++ means good (8 points), + means relatively good (6 points), ± means suitable (4 points), - means not good (2 points); whiteness: white (10 points), light yellow (8 points), dark yellow (6 points), brown (4 points), black (2 points); fineness: fine (10 points), medium fine (8 points), medium rough (6 points), rough (4 points), severe granularity (2 points); spreadability: good spreadability (5 points), poor spreadability (2 points), good dispersibility (5 points), poor dispersibility (2 points).

[0030] Stability investigation: The stability of Veratrum ointment was investigated from three aspects: centrifugation test, heat resistance test, and cold resistance test. Centrifugation test investigation: 10 g of the cream for removing keratinized tissue of the nipples of dairy cows in each group was centrifuged for 5 min (3000rpm) to observe whether there was water-oil stratification. Heat resistance test investigation: 15 g of the cream for removing keratinized tissue of the nipples of dairy cows in each group was placed in an ointment box, placed in an oven at a constant temperature (45C), taken out after 12 hours, and observed after cooling to room temperature. Cold resistance test investigation: 15 g of the cream for removing keratinized tissue of the nipples of dairy cows in each group was placed in an ointment box, covered with a lid, placed in a refrigerator (-20℃) for 12 hours, taken out, and observed after reaching room temperature. The three investigation indicators each accounted for 10 points, with a full score of 30 points. Scoring rules for centrifugal test: The full score is 10 points, and according to the state of stratification, it is divided into 3 levels: excellent (8~10 points), good (4~7 points), and poor (0~3 points). Scoring rules for heat resistance test: The full score is 10 points, and according to the state of stratification, it is divided into 3 levels: excellent (8~10 points), good (4~7 points), and poor (0~3 points). Scoring rules for cold resistance: The full score is 10 points, and it is scored in 5 index units: roughening, thinning, softening, blistering, and stratification, each with 2 points; high degree ++ (0 points), low degree + (1 point), no change - (2 points), and the accumulation is the cold resistance index score. The results are shown in Table 1.

[0031]

[0032] As can be seen from Table 1, in Example 2, no delamination, demulsification, mildew, color, uniformity change, etc. were observed in the cream in the stability test, centrifugation test, cold and heat resistance test, and accelerated test of the cream, and the exfoliating cream prescription in Example 2 is the best ratio. A plurality of exfoliating creams of the ratio in Example 2 were taken for quality inspection, and the results in Table 2 were obtained.

[0033]

[0034] Finally, a control test was conducted on the nipples of dairy cows. 20 dairy cows were selected for the test, with 5 cows in each group. The povidone-iodine bath solution group, the Chinese medicine cream group prepared by the formula of the present invention, the blank control group and the negative control group were used respectively. The results shown in Table 3 and Table 3 were obtained after the test. Figure 4 result.

[0035]

[0036] In the present invention, bletilla striata extract, glycerin, salicylic acid and jojoba oil are added to the exfoliating Chinese medicine cream. The bletilla striata extract has good local astringent hemostasis and swelling-reducing and muscle-generating effects. The glycerin has the effects of moisturizing, relieving dry skin, protecting the skin from external environmental stimulation and promoting the self-repair of damaged cells. The salicylic acid can dissolve the constituent substances between cuticles and make the cuticles fall off, so the cuticles that are accumulated too thick can be removed. The jojoba oil has the effects of moisturizing the skin, relieving dryness and enhancing the elasticity and moisturizing ability of the skin. Therefore, the cream prepared by the formula has the effects of exfoliating, moisturizing and moisturizing, and has a good treatment effect on the nipple skin of dairy cows.

Claims

1. An ointment for removing keratinized tissue of cow nipples, characterized in that: The invention is prepared from the following raw materials in the following weight ratios: 1-2 g of bletilla striata extract, 7.5 g of jojoba oil, 1.5 g of emulsifier, 0.5 g of glyceryl monostearate, 1 g of sodium PCA, 4.5 g of glycerol, 0.5 g of vitamin E, 3 g of salicylic acid, 1 g of peony bark extract, and the remaining distilled water is added to 50 g.

2. The cream for removing keratinized tissue of the nipple of a dairy cow according to claim 1, characterized in that: It is made from the following raw materials in the following weight ratio: 1.5g of Bletilla striata extract, 7.5g of jojoba oil, 1.5g of emulsifier, 0.5g of glyceryl monostearate, 1g of sodium PCA, 4.5g of glycerol, 0.5g of vitamin E, 3g of salicylic acid, 1g of peony bark extract, and the remaining distilled water is added to 50g.

3. A method for preparing an ointment for removing keratinized tissue of a cow nipple according to claim 1 or 2, characterized in that: The specific steps are as follows: S1: preparing the oil phase drug: heating jojoba oil, glyceryl monostearate and vitamin E until melted and then adding an emulsifier; S2: preparing aqueous phase medicine: heating the bletilla striata extract, salicylic acid, sodium PCA, glycerin and water until they are melted, and then adding the peony bark extract; S3: Mix the oil phase medicine and the water phase medicine and stir until they are uniform to obtain a finished cream.

4. Use of the cream according to claim 1 or 2 in removing keratinized tissue from the nipple of a dairy cow.

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