Platelet bacterium inactivation method

Abstract

The invention discloses a platelet bacterium inactivation method which is characterized by sequentially comprising the following steps: S1, collecting manual platelets, or aseptically connecting singly collected platelets with an illumination plate; s2, after the platelets flow through an illumination plate for illumination and bacterium inactivation, the platelets are stored in a storage bag, 254 nm short-wave ultraviolet radiation is adopted for illumination and bacterium inactivation, and the irradiation dose is 100-200 mJ / cm < 2 >. The short-wave ultraviolet irradiation is adopted, the platelet suspension is sterilized, the advantages of being high in sterilization efficiency, small in platelet loss and low in use cost are achieved, and compared with other photochemistry, no photosensitizer is added or left.

Description

Technical Field

[0001] The present application relates to bacterial inactivation, and in particular to a method for inactivating platelet bacteria. Background Art

[0002] Platelet transfusion is a very important treatment method in clinical practice. It can be used to treat thrombocytopenia caused by various diseases, as well as preoperative platelet enhancement, coagulation disorders and patients with massive blood transfusion. However, the current situation of platelet bacterial contamination is not optimistic. There are two main methods for preparing platelets. One is to collect platelets by manual mixing. Manual platelets need to be mixed and collected for multiple people at a time. The amount of white blood cells and red blood cells mixed in is relatively large, which is more likely to cause adverse reactions such as non-hemolytic fever reaction and alloimmunization, and cause immune platelet transfusion to be ineffective. At the same time, the bacterial contamination rate during the mixing and collection process is high, which is more likely to cause bacterial contamination. Another type of single-collection platelet is to set up a machine to collect according to the platelet count of the blood donor before blood donation. The quality is relatively stable and the risk of contamination is lower than that of manual platelet. However, any blood product may be contaminated by bacteria, and platelets are particularly susceptible to bacterial contamination because platelets cannot be frozen. In order to maintain physiological functions, platelets must be stored in a constant temperature oscillating box at 22±2℃. In addition, the rich nutrition of platelets is also conducive to bacterial growth. Once contaminated, bacteria will quickly multiply in them. Clinically, if bacterially contaminated blood is transfused, the patient will experience chills, fever, hypotension, nausea, vomiting, or even shock within a short period of time, causing bacteremia and resulting in a higher risk of clinical platelet transfusion therapy.

[0003] Platelets are a clinically scarce medical resource. Bacterial culture before clinical use is currently considered an effective detection method, but bacterial culture has true and false positive reactions. In order to reduce platelet bacterial contamination, reduce adverse reactions to blood transfusions, reduce false positives in platelet bacterial culture, and reduce unnecessary platelet waste, new platelet bacterial inactivation technologies have emerged. Irradiation is one of the effective bacterial inactivation technologies. Using a certain wavelength of light to irradiate blood and blood components can hinder bacterial nucleic acid replication and protein synthesis, thereby achieving bacterial inactivation.

[0004] In the existing light treatment technology, plastic blood bags suitable for the penetration of light of a certain wavelength are usually used to contain the blood to be treated and blood components for light inactivation of bacteria. This method has strict requirements on the liquid thickness of the treated blood and the light transmittance of the plastic blood bag, and also requires a longer light inactivation time. However, longer light exposure will cause damage to the blood components and the effective components of blood products.

[0005] More importantly, when plastic blood bags are used to hold the processed blood, the blood inside the blood bag may not be irradiated by light or the irradiation dose may be insufficient, which may affect the inactivation of bacteria. Even if the blood is shaken or shaken, it cannot be guaranteed that all the blood in the bag is irradiated in a controlled and quantitative manner, which may easily affect the inactivation of bacteria.

[0006] There is no mature platelet bacteria inactivation technology in China. The existing technology abroad is to add photosensitizers and combine them with photochemical reactions produced by different wavelengths of light to kill viruses or bacteria in blood or blood components. The disadvantage is that the added photosensitizers themselves are more or less toxic, and there are problems with their residues and metabolism in the human body. The more mature methylene blue (MB) method is potentially teratogenic and needs to be processed and removed, and it mainly targets viruses; while blood irradiation has the problem that the dose is difficult to control and the operation is difficult. The operator needs to be well protected, and most of them are bulky and have high investment costs. Summary of the invention

[0007] The object of the present invention is to provide a method for inactivating platelet bacteria.

[0008] To achieve the above object, the present invention provides the following technical solutions.

[0009] The present application discloses a method for inactivating platelet bacteria, which comprises the following steps in sequence:

[0010] S1 pools platelets or aseptically connects apheresis platelets to an irradiated plate;

[0011] S2 passes the pooled platelets or the single-collected platelets connected to the illumination board through the illumination board for light-inactivation of bacteria and then stores them in the storage bag. The light-inactivation adopts 254nm short-wave ultraviolet radiation with an irradiation dose of 100-200mJ / cm 2 ;

[0012] Preferably, in the above-mentioned platelet bacteria inactivation method, the inactivated bacteria include Streptococcus bovis ATCC 33317, Streptococcus pyogenes ATCC21059, Streptococcus dysgalactiae LTCC 27957, Listeria monocytogenes BWCC 772545, Streptococcus agalactiae ATCC12386, Staphylococcus epidermidis CMCC (B) 26069, Acinetobacter baumannii CICC (B) 25001, Bacillus thuringiensis BWCC29782, Proteus mirabilis CMCC (B) 49005), Staphylococcus aureus (Staphylococcus aureus CMCC (B) 26003), Pseudomonas fluorescens (Pseudomonasfluorescens ATCC13525), Escherichia coli (Escherichia coliATCC 25922), Serratia marcescens (Serratia marcescens HZB368354), Propionibacterium acnesATCC 6919, Clostridiumsporogenes (Clostridiumsporogenes CMCC (B) 64941), Klebsiellapneumoniae BWCC66641, Streptococcus pneumoniae GDMCC1.2749, Morganellamorganii ATCC 25830, Bacillus cereus CMCC(B)63301) and Enterobacter cloacae (Enterobacter cloacae CICC(B)45301) or more thereof.

[0013] Preferably, in the above-mentioned platelet bacteria inactivation method, a disposable platelet bacteria inactivation device is used in conjunction with a blood transfusion device, and the disposable platelet bacteria inactivation device is used in conjunction with a blood transfusion device, and the disposable platelet bacteria inactivation device is used in conjunction with a blood transfusion device, including:

[0014] A blind-end catheter is provided with multiple strands that merge into one line and is connected to the platelet bag to be collected through a sterile pipette;

[0015] A collection bag, wherein the multiple blind-end catheters are connected to the input end of the collection bag after being merged into the same pipeline, and is used to collect the merged platelets;

[0016] An illumination board, wherein the output end of the collection bag is connected to the input end of the illumination board through a pipeline, and the illumination board is a pipeline structure made of a light-transmitting material, and the platelets flow through the illumination board to receive light-induced bacterial inactivation treatment;

[0017] The storage bag has an output end of the illumination board connected to an input end of the storage bag through a pipeline. The storage bag is used for storing platelets after bacterial light inactivation and clinical platelet transfusion.

[0018] Preferably, in the above-mentioned platelet bacteria inactivation method, a sample retention bag is connected in a shunt manner on the pipeline between the illumination plate and the storage bag.

[0019] Preferably, in the above-mentioned platelet bacteria inactivation method, a first flow-stop clamp is provided on the same pipeline where the multiple blind-end catheters converge.

[0020] Preferably, in the above-mentioned platelet bacteria inactivation method, a second flow-stop clamp is provided on the pipeline between the collection bag and the illumination plate.

[0021] Preferably, in the above-mentioned platelet bacteria inactivation method, a third flow-stop clamp is provided on the pipeline diverting to the sample retention bag.

[0022] Preferably, in the above-mentioned platelet bacteria inactivation method, the input end and the output end of the collection bag are both opened at the top thereof.

[0023] Preferably, in the above-mentioned platelet bacteria inactivation method, the illumination plate is a flat plate with a serpentine pipe structure, the input end of the illumination plate is located at the top thereof, and the output end is located at the bottom thereof.

[0024] Preferably, in the above-mentioned platelet bacteria inactivation method, the storage bag is made of a special PVC film material, which has good air permeability and meets the storage requirements of the platelet physiological cycle.

[0025] The present invention adopts short-wave ultraviolet irradiation to sterilize platelet suspension, and has the advantages of high sterilization efficiency, small platelet loss and low use cost. Compared with other photochemical methods, no photosensitizer is added or left.

[0026] At the same time, the present invention designs a disposable blood transfusion device composed of a pipeline, an illumination board, and a blood bag (collection bag, storage bag, and sample bag). One of the core parts is a multi-strand blind-end catheter, and each single-strand blind-end catheter can be aseptically connected to a portion of platelets to be collected, reducing the risk of cross contamination during collection. The second core part is the illumination board, which is made of a special light-transmitting material. Here, the platelets are exposed to short-wave ultraviolet rays to kill possible bacteria. The third core part is the storage bag, which is made of a special PVC film material, has good air permeability, meets the preservation requirements of the platelet physiological cycle, and guarantees the storage performance of platelets under specified conditions. The whole set of equipment constitutes a closed passage. While preventing bacterial contamination, it is used in conjunction with the medical platelet bacterial inactivation equipment to accurately control the light irradiation dose of all platelets to be processed, achieving a safe and efficient bacterial inactivation effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 Shown is a schematic diagram of a blood transfusion device used in conjunction with a disposable platelet bacteria inactivation device in an embodiment of the present invention. DETAILED DESCRIPTION

[0029] The following will describe the technical solutions in the embodiments of the present invention in detail in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Combination Figure 1 As shown, the disposable blood transfusion equipment 100 used in conjunction with the platelet bacteria inactivation device comprises:

[0031] The blind-end catheter 101 is provided with multiple strands which converge into the same pipeline and is connected to the platelet bag to be collected through a sterile pipette machine;

[0032] A collection bag 102, where multiple blind-end catheters 101 are connected to the same pipeline and then connected to the input end of the collection bag 102, is used to collect the platelets;

[0033] The output end of the collection bag 102 is connected to the input end of the illumination board 103 through a pipeline. The illumination board 103 is a pipeline structure made of light-transmitting materials. The platelets flow through the illumination board 103 to receive light-induced bacterial inactivation treatment.

[0034] Storage bag 104, the output end of the illumination board 103 is connected to the input end of the storage bag 104 through a pipeline. The storage bag 104 is made of a special PVC film material, has good air permeability, and meets the preservation requirements of the platelet physiological cycle. The storage bag 104 is used for storing platelets after bacterial light inactivation and clinical platelet transfusion.

[0035] A sample bag 105 is connected to the pipeline between the illumination plate 103 and the storage bag 104 in a branch manner.

[0036] A first stopper 106 is provided on the same pipeline where multiple blind-end catheters 101 converge. A second stopper 107 is provided on the pipeline between the collection bag 102 and the illumination board 103. A third stopper 108 is provided on the pipeline that is diverted to the sample bag 105. The input end and the output end of the collection bag 102 are both opened at the top thereof. The illumination board 103 is a flat plate with a serpentine pipeline structure, and the input end of the illumination board 103 is located at the top thereof, and the output end is located at the bottom thereof.

[0037] Example 1: The present invention is applied to manual platelet collection and bacterial inactivation. The specific implementation is carried out according to the following steps:

[0038] Preparation before use:

[0039] (1) Collect platelets to be pooled from the blood bank.

[0040] (2) Before using this product, please check whether the packaging bag is damaged, whether it is within the shelf life, and whether the packaging bag has been opened. If any of the above conditions are not met, it should not be used.

[0041] (3) Open the packaging bag.

[0042] Manual platelet pooling:

[0043] (1) Close all stop clamps on the equipment and connect the blind-end catheter to the platelet bag to be collected using a sterile tube connection machine.

[0044] (2) Place the platelet bag to be collected at a high place to create a pressure difference with the collection bag, and open the first stop clamp.

[0045] (3) Collect the platelets into a collection bag.

[0046] Lighting process:

[0047] (1) Close the first stop clamp or heat-seal the discarded multi-strand blind-end catheter part, and hang the collection bag to be processed on the top hook of the matching "medical platelet bacteria inactivation cabinet"; the pipeline at the input end of the illumination board is inserted into the flow rate control bayonet corresponding to the top hook number; the illumination board is inserted into the illumination position corresponding to the top hook number; the pipeline at the output end of the illumination board hangs naturally, and the storage bag and the sample bag are laid flat in the corresponding oscillation frame at the bottom of the inactivation cabinet.

[0048] (2) Open the second stop clamp and start the "Medical Platelet Bacteria Inactivation Cabinet" to perform light inactivation according to the set program.

[0049] (3) After the light inactivation is completed, the platelets flow into the storage bag.

[0050] (4) Take out all the kits, heat-seal the pipes at the output end of the illumination board, discard the collection bag and illumination board, and place the storage bag and sample bag in a special platelet storage box and store them according to relevant requirements.

[0051] (5) When sampling and testing is required, the third stop clamp can be opened to collect the test sample from the storage bag to the sample retention bag. After sampling is completed, the input end catheter of the storage bag is heat-sealed.

[0052] (6) Storage bags containing bacteria-inactivated platelets can be used for direct clinical transfusion.

[0053] Example 2: The present invention is applied to the inactivation of bacteria in single-donor platelets. The specific processing steps are as follows:

[0054] (1) Obtain platelets from a blood bank.

[0055] (2) Before using this product, please check whether the packaging bag is damaged, whether it is within the shelf life, and whether the packaging bag has been opened. If any of the above conditions are not met, it should not be used.

[0056] (3) Open the packaging bag.

[0057] When used specifically:

[0058] Close all stop clamps on the equipment, connect the catheter at the input end of the illumination board to the single-collection platelet bag using a sterile pipetting machine, and discard the blind-end catheter and the collection bag.

[0059] Lighting process:

[0060] (1) Hang the single-collected platelet bag to be processed on the top hook of the matching "medical platelet bacterial inactivation cabinet"; the pipeline at the input end of the illumination board is inserted into the flow rate control bayonet corresponding to the number of the top hook; the illumination board is inserted into the illumination position corresponding to the number of the top hook; the pipeline at the output end of the illumination board hangs naturally, and the storage bag and the sample bag are laid flat in the corresponding oscillation frame at the bottom of the inactivation cabinet.

[0061] (2) Open the second stop clamp and start the "Medical Platelet Bacteria Inactivation Cabinet" to perform light inactivation according to the set program.

[0062] (3) After the light inactivation is completed, the platelets flow into the storage bag.

[0063] (4) Take out all the kits, heat-seal the pipes at the output end of the illumination board, discard the single-collected platelet bags and illumination board, and place the storage bag and sample bag in a special platelet storage box and store them according to relevant requirements.

[0064] (5) When sampling and testing is required, the third stop clamp can be opened to collect the test sample from the storage bag to the sample retention bag. After sampling is completed, the input end catheter of the storage bag is heat-sealed.

[0065] (6) Storage bags containing bacteria-inactivated platelets can be used for direct clinical transfusion.

[0066] The present invention is composed of pipelines, illumination plates, and blood bags (collection bags, storage bags, and sample bags). One of the core parts is a multi-strand blind-end catheter, and each single-strand blind-end catheter can be aseptically connected to a portion of platelets to be collected, reducing the risk of cross contamination during collection. The second core part is the illumination plate, which is made of a special light-transmitting material. Here, the platelets are exposed to short-wave ultraviolet radiation to kill possible bacteria. The third core part is the storage bag, which is made of a special PVC film material and has good air permeability. It meets the preservation requirements of the platelet physiological cycle and guarantees the storage performance of the platelets under specified conditions. The whole set of equipment constitutes a closed passage. While preventing bacterial contamination, it is used in conjunction with medical platelet bacterial inactivation equipment to accurately control the light irradiation dose of all platelets to be processed, achieving a safe and efficient bacterial inactivation effect.

[0067] The disposable platelet bacteria inactivation device is used in conjunction with blood transfusion equipment to inactivate platelet bacteria, including:

[0068] S1 collects manual platelets or aseptically connects apheresis platelets to an irradiated plate;

[0069] S2 passes the pooled platelets or the single-collected platelets connected to the illumination board through the illumination board for light-inactivation of bacteria and then stores them in the storage bag. The light-inactivation adopts 254nm short-wave ultraviolet radiation with an irradiation dose of 100-200mJ / cm 2 ;

[0070] Example 3: Experiments were conducted on the application of the platelet bacteria inactivation method provided by the present invention to verify the inactivation ability of platelet transfusion-related bacteria (WHO platelet transfusion-related bacteria reference strain repository) and the effect of UVC light treatment on platelet function. The specific processing steps are as follows:

[0071] Part I: Provide main reagents, raw materials and instruments

[0072] The bacteria were sourced from Guangdong Huankai Microbial Technology Co., Ltd. and Wuhan Huizao Biotechnology Co., Ltd.

[0073] The medical platelet bacteria inactivation cabinet was developed by “Lanbo Medical Technology Development (Suzhou) Co., Ltd.”

[0074] The blood transfusion equipment used in the disposable platelet bacteria inactivation device was developed by "Lanbo Medical Technology Development (Suzhou) Co., Ltd."

[0075] The blood cell analyzer and reagents are from Shenzhen Jinrui brand;

[0076] Platelet aggregation instruments and reagents are of the Telicon brand;

[0077] The UV spectrophotometer is a Beijing Puxi brand;

[0078] The remaining reagents and instruments are all conventional domestic models.

[0079] Part II: Bacterial inactivation and detection of platelets

[0080] 1. Preparation of platelet suspension:

[0081] (1) Take an appropriate amount of bacterial culture with an inoculation loop and add it to pH 7.0 sodium chloride peptone nutrient solution. After oscillation and mixing, measure the turbidity with a McFarland turbidimeter tube to obtain 3*10 8 CFU / ml bacterial suspension,

[0082] (2) The bacterial suspension was diluted in a gradient manner (10-fold dilution each time) and injected into the platelets to mix well to make 3*10 6 cfu / ml of contaminated platelets.

[0083] 2. Inactivation process:

[0084] S1. Take the disposable blood transfusion equipment kit for the platelet bacteria inactivation device and transfer the contaminated platelets to the collection bag of the inactivation kit;

[0085] S2. The medical platelet bacteria inactivation cabinet is powered on and self-checked to be ready for use;

[0086] S3. Hang the mixed bag containing the contaminated platelets on the upper hook in the inactivation box, insert the UVC irradiation component into the irradiation position of the irradiation unit, install the upper end conduit of the irradiation component on the flow control caliper, place the storage bag in the oscillation frame below the irradiation unit, and close the door of the irradiation box;

[0087] S4. Set the UVC light dose according to the verification plan and start the inactivation program;

[0088] S5, the infected platelets flow through the illumination component to be treated with UVC light and then return to the storage bag, thereby obtaining bacteria-inactivated platelets;

[0089] S6. Heat-seal the storage bag catheter and use a sterile syringe to extract a sample from the storage bag for inspection;

[0090] S7. After the treatment, all items that have come into contact with bacteria must be sterilized and inactivated at high temperature to prevent bacterial contamination.

[0091] 3. Collect the inactivated platelets, i.e., bacterially inactivated platelets, and take samples for inspection.

[0092] 4. Bacteria detection:

[0093] (1) Before inactivation, the platelets stained with bacteria were diluted with pH 7.0 sodium chloride peptone nutrient solution to a bacterial content of about 30 cfu / ml (100,000 times dilution), and 1 ml was taken and spread on about 20 ml of appropriate culture medium. After standing for 20 to 30 minutes, it was inverted and cultured in a 37°C incubator for 48 hours, and the plate was counted. The actual concentration of bacteria in the platelets stained with bacteria before inactivation was calculated.

[0094] (2) Take 1 ml of the inactivated platelets and spread it on 20 ml of culture medium. After standing for 20 to 30 minutes, invert it and culture it in a 37°C incubator for 48 hours. Count the plates and calculate the actual concentration of bacteria in the inactivated platelets.

[0095] (3) Calculate the logarithmic killing value of bacteria inactivated by treatment (log 10 ).

[0096] 5. Performance testing of platelets: before and after UVC irradiation treatment

[0097] (1) Blood cell analyzer detects changes in platelet count;

[0098] (2) Use ultraviolet spectrophotometer to detect changes in platelet hypotonic shock response;

[0099] (3) Use a platelet aggregation instrument to detect changes in platelet aggregation rate.

[0100] Table 1 Bacterial killing logarithm (log 10 )Statistical table

[0101]

[0102]

[0103] Table 2 Statistical table of platelet performance changes (percentage values ​​before and after comparison)

[0104] Serial number <![CDATA[Irradiation dose mJ / cm 2 > 50 100 150 200 250 1 Platelet count (PLT) 99％ 97％ 93％ 82％ 68％ 2 Platelet hypotonic shock (HSR) 95％ 92％ 88％ 81％ 70％ 3 Platelet aggregation rate 98％ 98％ 95％ 80％ 47％

[0105] Specifically, it can be seen from the above two tables that the greater the irradiation dose, the better the bacterial inactivation effect, but the greater the impact on platelet performance. 2 , it has excellent killing effect on reference bacteria in platelets; when UVC irradiation dose>200mJ / cm 2 The platelet recovery efficiency is less than 80%, so the irradiation dose is controlled at 100-200mJ / cm 2 It can minimize the damage to platelets while ensuring the highest bactericidal effect.

[0106] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0107] The above is only a specific implementation of the present application. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present application. These improvements and modifications should also be regarded as the scope of protection of the present application.

Claims

1. A method for inactivating platelet bacteria, characterized in that: The steps are as follows: S1 collects manual platelets or aseptically connects apheresis platelets to an irradiated plate; S2 The collected platelets or the single-collected platelets connected to the illumination board flow through the illumination board to inactivate bacteria and then are stored in the storage bag. The light inactivation adopts 254nm short-wave ultraviolet radiation with an irradiation dose of 100-200mJ / cm 2 .

2. The platelet bacteria inactivation method according to claim 1, characterized in that: The inactivated bacteria include Streptococcus bovis ATCC 33317, Streptococcus pyogenes ATCC21059, Streptococcus dysgalactiae LTCC 27957, Listeria monocytogenes BWCC 772545, Streptococcus agalactiae ATCC12386, Staphylococcus epidermidis CMCC (B) 26069, Acinetobacter baumannii CICC (B) 25001, Bacillus thuringiensis BWCC29782, Proteus mirabilis CMCC (B) 49005, Staphylococcus aureus CMCC (B) 26003), Pseudomonas fluorescens ATCC13525, Escherichia coli ATCC 25922, Serratia marcescens HZB368354, Propionibacterium acnes ATCC 6919, Clostridium sporogenes CMCC (B) 64941, Klebsiella pneumoniae BWCC 66641, Streptococcus pneumoniae GDMCC1.2749, Morganella morganii ATCC 25830, Bacillus cereus CMCC (B) 63301 and Enterobacter cloacae CICC (B) 45301.

3. The platelet bacteria inactivation method according to claim 1, characterized in that: The disposable platelet bacteria inactivation device is used in conjunction with the blood transfusion equipment, and the disposable platelet bacteria inactivation device is used in conjunction with the blood transfusion equipment, including: A blind-end catheter is provided with multiple strands that merge into one line and is connected to the platelet bag to be collected through a sterile pipette; A collection bag, wherein the multiple blind-end catheters are connected to the input end of the collection bag after being merged into the same pipeline, and is used to collect the merged platelets; An illumination board, wherein the output end of the collection bag is connected to the input end of the illumination board through a pipeline, and the illumination board is a pipeline structure made of a light-transmitting material, and the platelets flow through the illumination board to receive light-induced bacterial inactivation treatment; The storage bag has an output end of the illumination board connected to an input end of the storage bag through a pipeline. The storage bag is used for storing platelets after bacterial light inactivation and clinical platelet transfusion.

4. The platelet bacteria inactivation method according to claim 3, characterized in that: A sample bag is connected in a shunt manner on the pipeline between the illumination plate and the storage bag.

5. The platelet bacteria inactivation method according to claim 3, characterized in that: A first flow-stop clamp is arranged on the same pipeline where multiple blind-end catheters converge.

6. The platelet bacteria inactivation method according to claim 3, characterized in that: A second flow-stop clamp is arranged on the pipeline between the collecting bag and the illumination plate.

7. The platelet bacteria inactivation method according to claim 4, characterized in that: A third flow-stop clamp is arranged on the pipeline that is diverted to the sample retention bag.

8. The platelet bacteria inactivation method according to claim 3, characterized in that: The input end and the output end of the collecting bag are both opened at the top end thereof.

9. The platelet bacteria inactivation method according to claim 3, characterized in that: The illumination plate is a flat plate with a serpentine pipe structure, the input end of the illumination plate is located at the top thereof, and the output end is located at the bottom thereof.

10. The platelet bacteria inactivation method according to claim 3, characterized in that: The storage bag is made of a special PVC film material, has good air permeability, and meets the storage requirements of the platelet physiological cycle.

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