Micro-fluidic chip and anti-corrosion microorganism detection device

Abstract

The invention discloses a micro-fluidic chip and an anti-corrosion microorganism detection device, and relates to the technical field of biological detection.The micro-fluidic chip is used for shunt screening of anti-corrosion microorganisms and comprises a substrate and a chip body, the chip body is supported on the substrate and comprises a plurality of shunt screening channels sequentially arranged in the first direction, and the shunt screening channels are communicated with the substrate. Each shunting and screening channel comprises a confluence channel and a plurality of continuous phase channels, the confluence channel comprises an inflow port and an outflow port, and the continuous phase channels are communicated with the outflow port and the inflow port of the confluence channel in the adjacent shunting and screening channel; a flow dividing baffle is arranged in the confluence channel, the flow dividing baffle is arranged between the adjacent flow outlets and is opposite to one flow inlet in the first direction, and the distance between the flow dividing baffle and one flow outlet is equal to the distance between the flow dividing baffle and the other flow outlet. The technical scheme provided by the invention aims to more efficiently and quickly complete screening and detection of antiseptic microorganisms.

Description

Technical Field

[0001] The present invention relates to the field of biological detection technology, and in particular to a microfluidic chip and an antiseptic microorganism detection device. Background Art

[0002] Microbial corrosion is one of the main problems faced by metals in the atmosphere, water and soil environments, which may lead to reduced metal function, loss of structural integrity, major safety accidents and huge economic losses. However, with the continuous deepening of research on microbial corrosion, researchers have found that microorganisms can inhibit corrosion under certain conditions. There are many types of microorganisms, and the cultivation method is simple. They can reproduce in large quantities. Especially in complex environments and difficult construction or repair conditions, inhibiting corrosion through microorganisms will become one of the important means of corrosion prevention. However, the amount of anti-corrosion microorganisms that can be screened to inhibit corrosion is huge, and it is difficult to complete the screening of anti-corrosion microorganisms in a short period of time.

[0003] Therefore, there is an urgent need for a method for screening preservative microorganisms that can efficiently and quickly complete the screening and detection of preservative microorganisms. Summary of the invention

[0004] The main purpose of the present invention is to provide a microfluidic chip and an antiseptic microorganism detection device, aiming to complete the screening and detection of antiseptic microorganisms more efficiently and quickly.

[0005] To achieve the above-mentioned purpose, the present invention proposes a microfluidic chip, which is used for shunt screening of antiseptic microorganisms. The microfluidic chip includes a substrate and a chip body, wherein the chip body is supported on the substrate and includes a plurality of shunt screening channels arranged in sequence along a first direction, each shunt screening channel includes a confluence channel and a plurality of continuous phase channels, the confluence channel includes an inlet and an outlet, the continuous phase channel connects the outlet with the inlet of the confluence channel in an adjacent shunt screening channel; a shunt baffle is arranged in the confluence channel, the shunt baffle is arranged between adjacent outlets and opposite to an inlet along the first direction, and the spacing between the shunt baffle and one outlet is equal to the spacing between the shunt baffle and another outlet.

[0006] In some embodiments, the number of flow outlets of the confluence channel in any diversion screening channel is set to N, and the number of flow outlets of the confluence channel in the next diversion screening channel along the first direction is N+1.

[0007] In some embodiments, the continuous phase channel includes multiple straight segments and curved segments, the multiple straight segments are perpendicular to the first direction and are arranged in sequence along the first direction at intervals, the curved segments connect adjacent straight segments so that the multiple straight segments are connected to form a bent structure that is connected end to end, and the cross-sectional area of ​​the straight segment is equal to the cross-sectional area of ​​the curved segment.

[0008] In some embodiments, the continuous phase channel also includes a converging channel arranged at both ends, the converging channel is connected to the converging channel, the cross-sectional area of ​​the converging channel is larger than the cross-sectional area of ​​the straight line segment and smaller than the cross-sectional area of ​​the converging channel; at the connection between the converging channel and the converging channel, the cross-sectional area gradually decreases along the direction of the converging channel pointing to the converging channel.

[0009] In some embodiments, the microfluidic chip further includes a droplet inlet and an inlet tube connecting the droplet inlet and the inlet of the diversion screening channel; the cross-sectional area of ​​the inlet tube is equal to the cross-sectional area of ​​the continuous phase channel and smaller than the cross-sectional area of ​​the confluence channel.

[0010] In some embodiments, the inflow tube includes a first section and a second section that are connected, and there is an angle between the first section and the second section.

[0011] In some embodiments, the microfluidic chip also includes a plurality of droplet incubation channels and droplet outlets, the droplet incubation channel is connected to a diversion screening channel arranged at the end along the first direction, and the droplet outlet is connected to each continuous phase channel; the droplet incubation channel includes a main body and connecting parts arranged at both ends, and the cross-sectional area of ​​the droplet incubation channel gradually decreases along the direction from the main body to the connecting part.

[0012] The present application also provides an antiseptic microorganism detection device, which includes a plurality of microfluidic chips and detection components provided in any of the above embodiments; wherein: The detection component comprises a top plate layer, a sampling chamber layer and a sample layer which are stacked in sequence, the top plate layer is provided with a plurality of sampling channels, the sampling chamber layer is provided with a plurality of sampling chambers, and one sampling channel is connected to one sampling chamber; The sample layer comprises a communicating opening and a sample storage space, and the sample storage space is communicated with each sample injection chamber.

[0013] In some embodiments, a sample outlet channel is further provided in the top plate, one sample outlet channel is connected to one sample inlet chamber, and each sample outlet channel is used to connect to a waste liquid collection component.

[0014] In some embodiments, the antiseptic microorganism detection device further comprises a bottom plate layer, which is connected to the sample layer to provide support.

[0015] The technical solution of the present invention is to design the structure of the chip body, and set the chip body to include a plurality of diversion screening channels arranged in sequence along a first direction, each diversion screening channel includes a confluence channel and a plurality of continuous phase channels, and a diversion baffle is arranged in the confluence channel, the diversion baffle is arranged between adjacent flow outlets and is arranged opposite to a flow inlet along the first direction, the spacing between the diversion baffle and one flow outlet is equal to the spacing between the diversion baffle and another flow outlet, so as to form a space of the same volume on both sides of the confluence channel corresponding to the diversion baffle, and then the diversion baffle can be used to screen and divide the incoming antiseptic microbial sample. The structure is simpler and easier to operate, and it is more efficient when screening high-throughput antiseptic microbial samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0017] Figure 1 A schematic diagram of the three-dimensional structure of a microfluidic chip provided in one embodiment of the present invention; Figure 2 A schematic structural diagram of a chip body of a microfluidic chip provided in one embodiment of the present invention; Figure 3 for Figure 2 An enlarged view of part A of the microfluidic chip shown; Figure 4 A schematic diagram of the three-dimensional structure of a detection component in an antiseptic microorganism detection device provided in one embodiment of the present invention; Figure 5 A schematic diagram of the three-dimensional structure decomposition of a detection component in an antiseptic microorganism detection device provided in one embodiment of the present invention; Figure 6 for Figure 4 The shown device is a cross-sectional view of the antiseptic microorganism detection device along line BB.

[0018] Description of the accompanying drawings: 100, microfluidic chip; 10, substrate; 20, chip body; 21, diversion screening channel; 211, confluence channel; 2111, inlet; 2112, outlet; 2113, diversion baffle; 212, continuous phase channel; 2121, straight segment; 2122, curved segment; 2123, converging channel; 30, droplet inlet; 40, inflow tube; 41, first segment; 42, second segment; 50, droplet incubation channel; 51, main body; 52, connecting part; 60, droplet outlet; 200, detection component; 201, top plate layer; 2011, sample injection channel; 2012, counter electrode introduction channel; 2013, reference electrode introduction channel; 2014, sample outlet channel; 202, sample injection chamber layer; 2021, sample injection chamber; 203, sample layer; 2031, opening; 2032, sample storage space; 204, bottom plate layer.

[0019] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0022] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features limited to "first" and "second" may explicitly or implicitly include at least one of the features. In addition, if "and / or" or "and / or" appears in the full text, its meaning includes three parallel solutions. Taking "A and / or B" as an example, it includes solution A, solution B, or solutions that satisfy both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0023] Microbial corrosion is one of the main problems faced by metals in the atmosphere, water and soil environments, which may lead to reduced metal function, loss of structural integrity, major safety accidents and huge economic losses. However, with the continuous deepening of research on microbial corrosion, researchers have found that microorganisms can inhibit corrosion under certain conditions. There are many types of microorganisms, and the cultivation method is simple. They can reproduce in large quantities. Especially in complex environments and difficult construction or repair conditions, inhibiting corrosion through microorganisms will become one of the important means of corrosion prevention. However, the amount of anti-corrosion microorganisms that can be screened to inhibit corrosion is huge, and it is difficult to complete the screening of anti-corrosion microorganisms in a short period of time.

[0024] The microfluidic system is a platform that integrates multiple functional modules and can integrate conventional biochemical reactions into a tiny chip. The biggest advantage of microfluidics is that it can flexibly combine and integrate high-throughput experiments on a small platform, and efficiently complete the screening of large volumes of microbial samples. However, in order to obtain a reaction system with a specific ratio, complex control valves and branch devices are usually required. In addition, there is a lack of devices that can simultaneously perform functional testing on samples obtained from the corresponding high-throughput screening.

[0025] Please refer to Figures 1 to 3 The present invention proposes a microfluidic core 100, which is used for shunt screening of antiseptic microorganisms. The microfluidic chip 100 includes a substrate 10 and a chip body 20, wherein the chip body 20 is supported on the substrate 10 and includes a plurality of shunt screening channels 21 arranged in sequence along a first direction X, each shunt screening channel 21 includes a confluence channel 211 and a plurality of continuous phase channels 212, the confluence channel 211 includes an inlet 2111 and an outlet 2112, the continuous phase channel 212 connects the outlet 2112 with the inlet 2111 of the confluence channel 21 in the adjacent shunt screening channel 21; a shunt baffle 2113 is arranged in the confluence channel 211, the shunt baffle 2113 is arranged between adjacent outlets 2112 and opposite to an inlet 2111 along the first direction X, and the distance between the shunt baffle 2113 and one outlet 2112 is equal to the distance between the shunt baffle 2113 and another outlet 2112.

[0026] When using microfluidic chips for high-throughput screening of antiseptic microorganisms, there are problems such as low efficiency and uneven flow distribution. Traditional methods usually rely on complex control valves and branching devices, which are difficult to achieve efficient and rapid microbial screening. In order to overcome these limitations, the present invention proposes a microfluidic chip 100, which is intended to achieve efficient and rapid screening and equal distribution of antiseptic microorganisms.

[0027] Among them, the function of the substrate 10 is to provide support. In these embodiments of the present application, the substrate 10 can be made of glass, and the chip body 20 can be made of polydimethylsiloxane (PDMS). During the production stage, the chip body 20 made of PDMS material can be bonded to the substrate 10 to achieve connection between the substrate 10 and the chip body 20.

[0028] The chip body 20 includes a plurality of flow diversion and screening channels 21 sequentially arranged along the first direction X, wherein the plurality of flow diversion and screening channels 21 are an integrated structure and can be integrally formed during processing.

[0029] The function of the diversion screening channel 21 is to gradually divert the high-throughput antiseptic microorganism sample to eventually form a plurality of independent antiseptic microorganism sample sets.

[0030] In these embodiments of the present application, multiple flow-dividing and screening channels 21 can be defined as multiple flow-dividing levels of the microfluidic chip 100. That is, the flow-dividing and screening channels 21 in the first row can be defined as primary channels, the flow-dividing and screening channels 21 in the second row can be defined as secondary channels, and the flow-dividing and screening channels 21 in the third row can be defined as tertiary channels, so as to gradually divide the original high-throughput antiseptic microorganism sample into multiple sample sets with the same or different concentrations to be tested, and then perform different tests in the subsequent detection components for the antiseptic microorganism samples.

[0031] As a result, in these embodiments of the present application, a plurality of diversion and screening channels 21 are provided, so that the originally high-throughput antiseptic microbial samples can be diverted and screened step by step, thereby improving the quality of the diversion and screening while meeting the requirements for high-throughput screening.

[0032] For example, in these embodiments of the present application, the number of the shunt screening channels 21 can be set to five, so that after the high-throughput original droplets are screened at multiple levels, six antiseptic microbial sample sets with the same concentration or a concentration gradient are formed. Of course, this is not a limitation of the present application. In other embodiments of the present application, the number of the shunt screening channels 21 can also be set to two, three or four, which can be selected according to the purpose of the experiment and the subsequent test items.

[0033] Each of the diversion and screening channels 21 includes a confluence channel 211 and a plurality of continuous phase channels 212 , which means that the structures of the diversion and screening channels 21 are similar, and are all composed of a confluence channel 211 and a plurality of continuous phase channels 212 .

[0034] It should be noted that, since the microfluidic chip 100 provided in the present application adopts a step-by-step screening method to equally divide the antiseptic microbial sample, there are differences between different diversion screening channels 21. Specifically, the diversion levels applied to different diversion screening channels 21 can be differentiated in design, and then the structural size of the confluence channel 211 and the number of continuous phase channels 212 can be differentiated in design.

[0035] For example, in some embodiments, the length dimension of the confluence channel 211 in the diversion and screening channel 21 serving as the primary channel, the secondary channel, and the tertiary channel can be gradually increased along the first direction X, and the number of the continuous phase channels 212 also gradually increases accordingly.

[0036] The confluence channel 211 includes an inlet 2111 and an outlet 2112, and the continuous phase channel 212 connects the outlet 2112 with the inlet 2111 of the confluence channel 211 in the adjacent diversion and screening channel 21. The inlet 2111 and the outlet 2112 are interfaces in the confluence channel 211 for communicating with different continuous phase channels 212. In these embodiments of the present application, the inlets 2111 and the outlets 2112 of different diversion and screening channels 21 can be arranged at both ends of the first direction X, so that the antiseptic microbial sample flows in a single direction in the microfluidic chip 100 and is screened and divided step by step.

[0037] A diverter baffle 2113 is provided in the confluence channel 211. The diverter baffle 2113 is arranged between adjacent flow outlets 2112 and is arranged opposite to a flow inlet 2111 along the first direction X. In this way, between different rows of diverter screening channels 21, when the preservative microorganism sample flows from the front row to the back row, it is first diverted to both sides under the action of the diverter baffle 2113, and then is equally divided on both sides of the diverter baffle 2113.

[0038] The distance between the diverter baffle 2113 and one flow outlet 2112 is equal to the distance between the baffle and the other flow outlet 2112. The purpose of this setting is to control the concentration of the antiseptic microbial sample on both sides of the diverter baffle 2113, so that when subsequent antiseptic microbial samples flow in, under the premise of the same flow rate, the concentration of the antiseptic microbial samples flowing to both sides of the diverter baffle 2113 can be kept the same.

[0039] The technical solution of the present invention is to design the structure of the chip body 20, and set the chip body 20 to include a plurality of diversion screening channels 21 arranged in sequence along the first direction X, each diversion screening channel 21 includes a confluence channel 211 and a plurality of continuous phase channels 212, and a diversion baffle 2113 is arranged in the confluence channel 211, the diversion baffle 2113 is arranged between adjacent flow outlets 2112 and is arranged opposite to a flow inlet 2111 along the first direction X, the spacing between the diversion baffle 2113 and one flow outlet 2112 is equal to the spacing between the diversion baffle 2113 and another flow outlet 2112, so as to form a space of the same volume on both sides of the confluence channel 211 corresponding to the diversion baffle 2113, and then the diversion baffle 2113 can be used to screen and divide the incoming antiseptic microbial sample equally, the structure is simpler and easier to operate, and it is more efficient when screening high-throughput antiseptic microbial samples.

[0040] In some embodiments, the number of the outlets 2112 of the converging channel 211 in any diversion screening channel 21 is set to N, and the number of the outlets 2112 of the converging channel 211 in the next diversion screening channel 21 along the first direction X is N+1.

[0041] That is to say, between adjacent diverter screening channels 21, the difference in the number of outlets 2112 of two diverter screening channels 21 is 1, that is, the number of outlets 2112 in the diverter screening channel 21 in the rear row is one more than the number of outlets 2112 in the diverter screening channel 21 in the front row. In this way, between adjacent diverter screening channels 21, there must be at least one space separated by the diverter baffle 2113 in the diverter screening channel 21 in the rear row, which is connected with the two continuous phase channels 212 in the front row of diverter screening channels 21, so as to receive half of the antiseptic microorganism sample from each of the two continuous phase channels 212.

[0042] Based on this, in these embodiments of the present application, in addition to using the chip body 20 to equally screen high-throughput antiseptic microorganism samples, such a structure can also be used to control the concentration of the antiseptic microorganism samples, and then after the antiseptic microorganism samples flow through the last row of diversion screening channels 21, multiple antiseptic microorganism sample sets with a certain concentration gradient are formed.

[0043] For example, in some embodiments, the structure of the shunt screening channels 21 located in the first row can be designed so that in the shunt screening channels 21 in the first row, the concentrations of the antiseptic microorganism samples flowing through the confluence channel 211 to the two continuous phase channels 212 are different. In this way, after the antiseptic microorganism samples are shunt screened by multiple shunt screening channels 21, there are differences in the concentrations of the antiseptic microorganism samples flowing out through different continuous phase channels 212 in the shunt screening channels 21 in the last row, thereby forming a concentration gradient. When the subsequent detection component detects the antiseptic microorganism samples, the antiseptic effect can be detected for antiseptic microorganism samples of different concentrations after controlling other variables.

[0044] In some embodiments, the continuous phase channel 212 includes a plurality of straight segments 2121 and curved segments 2122, wherein the plurality of straight segments 2121 are perpendicular to the first direction X and are arranged in sequence along the first direction X at intervals, and the curved segments 2122 connect adjacent straight segments 2121 so that the plurality of straight segments 2121 are connected to form a bent structure connected end to end, and the cross-sectional area of ​​the straight segment 2121 is equal to the cross-sectional area of ​​the curved segment 2122.

[0045] The design of the straight segment 2121 and the curved segment 2122 of the continuous phase channel 212 enables the continuous phase channel 212 to realize a long path of fluid flow in a limited space. The straight segments 2121 are perpendicular to the first direction X and are arranged in sequence at intervals, so that the fluid can connect adjacent straight segments 2121 through the curved segments 2122 during the flow process. Through this design, the channel forms a bending structure that connects end to end, ensuring that the fluid flows in the continuous phase channel 212 for a longer time, thereby allowing the high-throughput antiseptic microbial samples to be arranged in a formation similar to one after another in the continuous phase channel 212 for a longer time, reducing the probability of antiseptic microorganisms accumulating in the continuous phase channel 212, and facilitating the use of the diversion baffle 2113 to gradually divide the high-throughput antiseptic microbial samples into equal parts. The cross-sectional areas of the straight segment 2121 and the curved segment 2122 are equal, ensuring the stability of the flow rate and pressure of the fluid during the flow process.

[0046] The continuous phase channel 212 of the present application realizes long-path flow in a limited space by combining multiple straight segments 2121 and curved segments 2122. The straight segments 2121 are perpendicular to the first direction X and are arranged at intervals, and the curved segments 2122 connect adjacent straight segments 2121, so that the fluid can flow smoothly. This structural design allows the fluid to flow longer in the channel, which is conducive to the arrangement and equal division of high-throughput antiseptic microbial samples. The cross-sectional areas of the straight segments 2121 and the curved segments 2122 are equal, ensuring the stability of the fluid during the flow process.

[0047] Through this design, the present application effectively solves the problem of how to improve the efficiency and accuracy of aliquoting high-throughput antiseptic microbial samples using the continuous phase channel 212. Compared with the prior art, the design of the present application achieves a longer flow path in a limited space, ensures the stability of fluid flow and the arrangement order of high-throughput samples, thereby improving the efficiency and accuracy of aliquoting.

[0048] In some embodiments, the continuous phase channel 212 also includes a converging channel 2123 arranged at both ends, the converging channel 2123 is connected to the converging channel 211, and the cross-sectional area of ​​the converging channel 2123 is larger than the cross-sectional area of ​​the straight segment 2121 and smaller than the cross-sectional area of ​​the converging channel 211; at the connection between the converging channel 2123 and the converging channel 211, the cross-sectional area gradually decreases along the direction of the converging channel 211 pointing to the converging channel 2123.

[0049] The continuous phase channel 212 includes a converging channel 2123, which is connected to the converging channel 211, and the cross-sectional area of ​​the converging channel 2123 is larger than the cross-sectional area of ​​the straight segment 2121, and smaller than the cross-sectional area of ​​the converging channel 211; at the connection between the converging channel 2123 and the converging channel 211, the cross-sectional area gradually decreases along the direction from the converging channel 211 to the converging channel 2123, so as to form a structure similar to a "horn". These features work together to facilitate the injection of the antiseptic microorganisms into the converging channel 211 and the continuous phase channel 212 through each diversion and screening channel 21 along the first direction X under the drive of the driving member (peristaltic pump), thereby ensuring the stability and high efficiency of the fluid during the flow process, and solving the problem of poor circulation of high-throughput antiseptic microorganisms at the connection between the continuous phase channel 212 and the converging channel 211.

[0050] The setting of the converging channel 2123 can be achieved in a variety of ways. For example, the desired converging channel 2123 structure can be directly formed on the chip body 20 by micro-machining technology, or the chip body 20 with the converging channel 2123 can be manufactured by mold injection molding technology. The cross-sectional area of ​​the converging channel 2123 can be changed by adjusting the processing parameters or the mold design. In addition, in order to further improve the stability of the fluid flow, a microstructure can be set on the inner wall of the converging channel 2123 to reduce the turbulence of the fluid during the flow process.

[0051] The present application sets a converging channel 2123 at both ends of the continuous phase channel 212, and makes the converging channel 2123 communicate with the converging channel 211, and the cross-sectional area of ​​the converging channel 2123 is larger than the cross-sectional area of ​​the straight segment 2121, and smaller than the cross-sectional area of ​​the converging channel 211. At the connection between the converging channel 2123 and the converging channel 211, the cross-sectional area gradually decreases along the direction from the converging channel 211 to the converging channel 2123, thereby forming a "trumpet"-shaped structure. In this way, the problem of poor circulation of high-throughput antiseptic microorganisms at the connection between the continuous phase channel 212 and the converging channel 211 is solved, and the stability and efficiency of the fluid during the flow process are ensured. Compared with the prior art, the technical solution of the present application has the advantages of simple structure, low manufacturing cost, and stable fluid flow.

[0052] In some embodiments, the microfluidic chip 100 also includes a droplet inlet 30 and an inlet tube 40 connecting the droplet inlet 30 and the inlet 2111 of the diversion screening channel 21; the cross-sectional area of ​​the inlet tube 40 is equal to the cross-sectional area of ​​the continuous phase channel 212 and is smaller than the cross-sectional area of ​​the confluence channel 211.

[0053] The droplet inlet 30 is a channel for external antiseptic microbial samples to flow in. When operating, the experimenter can connect the culture dish with the droplet inlet 30 through a pipeline, and then drive the antiseptic microbial sample in the culture dish to flow toward the droplet inlet 30 through a peristaltic pump arranged on the channel.

[0054] In these embodiments of the present application, the number of droplet inlets 30 and the concentration of the antiseptic microorganism samples in the culture dish can be selected according to the concentration requirements of different antiseptic microorganism sample sets after passing through the last row of diversion and screening pipes 21.

[0055] It should be noted that when the concentrations of different antiseptic microbial samples need to be controlled to be the same after passing through the last row of diversion screening channels 21, the number of droplet inlets 30 can be unrestricted, and the concentrations of the antiseptic microbial samples in different culture dishes can be controlled to be the same; and when the concentrations of different antiseptic microbial samples need to be controlled to have a certain concentration gradient after passing through the last row of diversion screening channels 21, the number of droplet inlets 30 can be set to at least two, and the concentrations of the antiseptic microbial samples in different culture dishes connected to different droplet inlets 30 can be different.

[0056] For example, in these embodiments of the present application, the number of droplet inlets 30 can be set to two, and the two droplet inlets 30 are connected to the confluence channel 211 of the first row of diversion screening channels 21 through different inflow pipes 40, respectively. At this time, the concentrations of the antiseptic microorganism samples in the two culture dishes can be set to 100% and 0%, respectively. In this way, after being screened by the first row of diversion screening channels 21, the concentrations of the antiseptic microorganism samples flowing into the next row of diversion screening channels 21 through the two continuous phase channels 212 are 100% and 0%, respectively; in the second row of diversion screening channels 21, after passing through the two diversion baffles 2113 After the diversion screening, in the three continuous phase channels 212 of the second row of diversion screening channels 21, the concentrations of the antiseptic microbial samples in the two continuous phase channels 212 on both sides are 100% and 0% respectively, while the continuous phase channel 212 in the middle is connected to the two continuous phase channels 212 in the previous row due to the space separated by the diversion baffle 2113 through the confluence channel 211 connected to it. At this time, the concentration of the antiseptic microbial sample is neutralized to 50% in this space; and so on, after the diversion screening of multiple diversion screening channels 21, multiple antiseptic microbial sample sets with increasing or decreasing concentrations can be obtained in the last row.

[0057] The inflow tube 40 is used to allow the antiseptic microbial sample to flow into the first row of the diversion screening channel 21. The cross-sectional area design of the droplet inlet 30 and the inflow tube 40 enables the droplets to maintain a stable flow rate and flow rate before entering the diversion screening channel 21, thereby improving the screening efficiency. The cross-sectional area of ​​the inflow tube 40 is equal to the cross-sectional area of ​​the continuous phase channel 212, which ensures that the droplets will not experience a sudden change in flow rate when entering the continuous phase channel 212, thereby avoiding interference with the screening process. At the same time, the cross-sectional area of ​​the inflow tube 40 is smaller than the cross-sectional area of ​​the confluence channel 211, which ensures that the droplets can be smoothly diverted when entering the confluence channel 211, thereby improving the screening efficiency.

[0058] Specifically, the inflow tube 40 can be made of different materials and manufacturing processes, such as by micro-machining technology. The shape and length of the inflow tube 40 can be adjusted according to actual needs to ensure that the droplets can smoothly enter the diversion screening channel 21. In addition, the design of the inflow tube 40 can also be optimized according to different microbial samples to improve the screening efficiency.

[0059] The present application designs a droplet inlet 30 and an inflow tube 40 in the microfluidic chip 100, so that the droplets can smoothly enter the diversion screening channel 21, thereby efficiently screening the antiseptic microorganisms. Compared with the prior art, the present application does not require complex control valves and branch devices, simplifies the design and manufacturing process of the microfluidic chip 100, and improves the screening efficiency and reliability.

[0060] In some embodiments, the inlet pipe 40 includes a first section 41 and a second section 42 that are connected to each other, and there is an angle between the first section 41 and the second section 42 .

[0061] The technical feature of the present application lies in the design of the inflow tube 40. The inflow tube 40 is composed of two interconnected sections, and there is an angle between the two sections. This design can effectively guide the fluid into the diversion screening channel 21 of the microfluidic chip 100. By setting the angle, the flow path of the fluid can be optimized, the adhesion performance of the pipeline wall to the droplet can be further increased at the droplet inlet 30, and the influence of external factors on the flow of the fluid in the microfluidic chip 100 can be reduced, thereby improving the screening efficiency and accuracy. This design can not only ensure the smooth flow of the fluid, but also reduce the retention and turbulence of the fluid in the inflow tube to a certain extent, thereby improving the performance of the entire microfluidic system.

[0062] The angle between the first section 41 and the second section 42 of the inflow tube 40 can be adjusted according to specific application requirements. For example, the angle can be set between 60° and 120° to adapt to the flow characteristics of different fluids and the design requirements of the microfluidic chip 100. In these embodiments of the present application, the angle can be set to 90°.

[0063] The length and diameter of the first section 41 and the second section 42 can also be adjusted according to specific needs to further optimize the flow path and flow efficiency of the fluid. As a preferred embodiment, the first section 41 can be designed as a straight section, and the second section 42 can be designed as a curved section to achieve a smoother fluid transition.

[0064] The design of the inflow tube 40 of the present application effectively solves the flow guidance problem of the fluid in the microfluidic chip 100 by setting an angle. Compared with the prior art, the design of the present application can optimize the flow path of the fluid without adding additional complex structures, improve screening efficiency and accuracy, reduce fluid retention and turbulence, and thus improve the overall performance of the microfluidic system.

[0065] In some embodiments, the microfluidic chip 100 also includes a plurality of droplet incubation channels 50 and droplet outlets 60. The droplet incubation channel 50 is connected to a diversion screening channel 21 disposed at the end along the first direction X, and the droplet outlet 60 is connected to each continuous phase channel 212. The droplet incubation channel 50 includes a main body 51 and connecting parts 52 disposed at both ends. Along the direction from the main body 51 to the connecting part 52, the cross-sectional area of ​​the droplet incubation channel 50 gradually decreases.

[0066] Through this design, the microfluidic chip 100 can make the antiseptic microorganisms stay at the end of the microfluidic chip 100, that is, at the end close to the droplet outlet 60, during the screening of antiseptic microorganisms, so that the concentration of the antiseptic microorganisms in the droplet incubation channel 50 gradually increases before flowing out through the droplet outlet, thereby improving the accuracy of subsequent detection of antiseptic microorganisms and reducing the possibility of errors in the detection results due to too low concentration. This structural design not only improves the screening efficiency, but also ensures the accuracy of subsequent detection results, thereby effectively solving the technical problem of efficient and rapid screening of high-throughput antiseptic microorganisms.

[0067] The design of the main body 51 and the connecting portion 52 of the droplet incubation channel 50 can be implemented in a variety of ways. For example, the main body 51 can be linear, and the connecting portion 52 can be conical, so as to achieve the effect of gradually reducing the cross-sectional area. Another implementation is that both the main body 51 and the connecting portion 52 are curved, but the curvature of the connecting portion 52 is larger, so that the droplets gradually shrink when flowing through the connecting portion. In addition, the design of the droplet outlet can also be diversified, for example, a porous structure can be used so that the droplets can flow out smoothly and enter the continuous phase channel 212.

[0068] Therefore, by optimizing the design of the droplet incubation channel 50 and the droplet outlet 60, the present application not only improves the efficiency of antiseptic microorganism screening, but also significantly improves the accuracy of subsequent detection. Compared with the prior art, the present application can achieve efficient and accurate antiseptic microorganism screening and detection without increasing the complexity of the system, solving the problems of low antiseptic microorganism screening efficiency and poor detection accuracy in the prior art.

[0069] Please refer to Figures 1 to 6 The present application also provides an antiseptic microorganism detection device (not numbered), which includes a plurality of microfluidic chips 100 and a detection component 200 as provided in any of the above embodiments; wherein, The detection component 200 includes a top plate layer 201, an injection chamber layer 202 and a sample layer 203 which are stacked in sequence. The top plate layer 201 is provided with a plurality of injection channels 2011, and the injection chamber layer 202 is provided with a plurality of injection chambers 2021. One injection channel 2011 is connected to one injection chamber 2021; the sample layer 203 includes a connected opening 2031 and a sample storage space 2032, and the sample storage space 2032 is connected to each injection chamber 2021.

[0070] In these embodiments of the present application, the antiseptic microorganism detection device can be used to screen and detect a large amount of antiseptic microorganisms to meet the needs of screening and detecting high-throughput biological samples. In actual operation, the microfluidic chips 100 provided in the above embodiments can be connected to the detection component 200, so that after the microfluidic chip 100 performs diversion and screening on the antiseptic microorganism sample, it is passed into the detection component 200 for different performance tests.

[0071] In these embodiments of the present application, a detection component 200 may be provided to simultaneously detect a plurality of microfluidic chips 100 to further meet the demand for high throughput.

[0072] Among them, the top plate layer 201 is provided with multiple injection channels 2011, that is, the injection channel 2011 is used to connect with the aforementioned microfluidic chip 100, so as to introduce the antiseptic microorganism sample with a certain concentration after screening and diversion into the detection component 200 through the droplet outlet of the microfluidic chip 100.

[0073] An injection channel 2011 is connected to an injection chamber 2021, which means that after the metal sample is placed into the sample storage space 2032 through the aforementioned opening 2031, the bottom of the injection chamber 2021 can be sealed. In this way, an injection channel 2011 and an injection chamber 2021 can form an independent reaction detection space to perform different experimental tests on different antiseptic microbial samples obtained through the diversion screening of the aforementioned microfluidic chip 100.

[0074] It can be understood that in these embodiments of the present application, the top plate 201 should also be provided with a counter electrode introduction channel 2012 and a reference electrode introduction channel 2013. At the same time, a counter electrode introduction channel 2012 and a reference electrode introduction channel 2013 are connected to a sampling chamber 2021, so that when conducting experimental tests, a counter electrode and a reference electrode can be placed in the sampling chamber 2021 respectively to provide support for the work of the antiseptic microbial sample in the sampling chamber 2021.

[0075] The injection chamber layer 202 is provided with a plurality of injection chambers 2021, wherein the injection chamber 2021 is a chamber for storing the introduced antiseptic microorganism samples, and different injection chambers 2021 are independent of each other so as to respectively detect the screened and equally divided antiseptic microorganism samples.

[0076] The sample layer 203 includes a connected opening 2031 and a sample storage space 2032, and the sample storage space 2032 is connected to each injection chamber 2021. After the metal sample is placed through the opening 2031, it can cooperate with the aforementioned multiple injection chambers 2021 to form independent detection chambers, and then the antiseptic microorganism samples in different detection chambers can be tested for the antiseptic performance of the metal sample.

[0077] In these embodiments of the present application, through the structural design of the detection component 200, after the metal sample is placed in, the detection environments between different detection cavities are independent of each other, and the metal sample used to detect the antiseptic effect of the antiseptic microbial sample is a whole piece. In this way, the detection error of the antiseptic effect of the antiseptic microbial sample caused by the difference in metal samples can be reduced, and the reliability and accuracy of the detection can be effectively improved.

[0078] In some embodiments, a sample outlet channel 2014 is further provided in the top plate 201 , one sample outlet channel 2014 is connected to one sample inlet chamber 2021 , and each sample outlet channel 2014 is used to connect to a waste liquid collection component.

[0079] In this way, in each independent detection chamber, the antiseptic microbial sample flowing in through the injection channel 2011 can be directly discharged through the sample outlet channel 2014 driven by the peristaltic pump after detection, thereby improving the continuity of the detection experiment, further meeting the needs of large-throughput diversion screening and detection, and improving reliability.

[0080] In some embodiments, the antiseptic microorganism detection device further includes a bottom plate layer 204, which is connected to the sample layer 203 to provide support to make the structure of the antiseptic microorganism detection device more stable.

[0081] The above are only preferred embodiments of the present invention, and are not intended to limit the patent scope of the present invention. All equivalent structural changes made using the contents of the present invention's specification and drawings, or directly / indirectly applied in other related technical fields, are included in the patent protection scope of the present invention.

Claims

1. A microfluidic chip for the shunt screening of antiseptic microorganisms, characterized in that: include: substrate; The chip body is supported on the substrate and includes a plurality of flow diversion and screening channels arranged in sequence along a first direction, each of the flow diversion and screening channels includes a confluence channel and a plurality of continuous phase channels, the confluence channel includes an inflow port and an outflow port, and the continuous phase channel connects the outflow port with the inflow port of the confluence channel in the adjacent flow diversion and screening channels; A diverter baffle is disposed in the converging channel. The diverter baffle is disposed between adjacent flow outlets and is disposed opposite to one of the flow inlets along the first direction. The distance between the diverter baffle and one of the flow outlets is equal to the distance between the diverter baffle and another of the flow outlets.

2. The microfluidic chip according to claim 1, characterized in that: Assume that the number of the flow outlets of the confluence channel in any of the diversion and screening channels is N, and then the number of the flow outlets of the confluence channel in the next diversion and screening channel along the first direction is N+1.

3. The microfluidic chip according to claim 1, characterized in that: The continuous phase channel includes a plurality of straight segments and curved segments, wherein the plurality of straight segments are perpendicular to the first direction and are arranged in sequence along the first direction at intervals, and the curved segments connect adjacent straight segments so that the plurality of straight segments are connected to form a bent structure connected end to end, and the cross-sectional area of ​​the straight segment is equal to the cross-sectional area of ​​the curved segment.

4. The microfluidic chip according to claim 3, characterized in that: The continuous phase channel further comprises converging channels arranged at both ends, the converging channels are connected to the converging channels, and the cross-sectional area of ​​the converging channels is larger than the cross-sectional area of ​​the straight line segment and smaller than the cross-sectional area of ​​the converging channels; At the connection between the converging channel and the converging channel, the cross-sectional area gradually decreases along the direction from the converging channel to the converging channel.

5. The microfluidic chip according to claim 1, characterized in that: The microfluidic chip also includes a droplet inlet and an inflow tube connecting the droplet inlet and the inflow inlet of the diversion screening channel; The cross-sectional area of ​​the inflow pipe is equal to the cross-sectional area of ​​the continuous phase channel and smaller than the cross-sectional area of ​​the confluence channel.

6. The microfluidic chip according to claim 5, characterized in that: The inflow pipe includes a first section and a second section that are connected, and an angle is formed between the first section and the second section.

7. The microfluidic chip according to claim 1, characterized in that: The microfluidic chip further comprises a plurality of droplet incubation channels and droplet outlets, wherein the droplet incubation channels are connected to the flow separation and screening channels arranged at the end along the first direction, and the droplet outlets are connected to each of the continuous phase channels; The droplet incubation channel includes a main body and connecting parts arranged at two ends. Along the direction from the main body to the connecting parts, the cross-sectional area of ​​the droplet incubation channel gradually decreases.

8. A device for detecting antiseptic microorganisms, characterized in that: It comprises a plurality of microfluidic chips and detection components as described in any one of claims 1 to 7; wherein, The detection assembly comprises a top plate layer, an injection chamber layer and a sample layer stacked in sequence, the top plate layer is provided with a plurality of injection channels, the injection chamber layer is provided with a plurality of injection chambers, and one of the injection channels is connected to one of the injection chambers; The sample layer comprises a communicating opening and a sample storage space, and the sample storage space is communicated with each of the injection chambers.

9. The antiseptic microorganism detection device according to claim 8, characterized in that: The top plate is also provided with a sample outlet channel, one of the sample outlet channels is communicated with one of the sample inlet chambers, and each of the sample outlet channels is used to connect to a waste liquid collection component.

10. The antiseptic microorganism detection device according to claim 8, characterized in that: The antiseptic microorganism detection device also includes a bottom plate layer, which is connected to the sample layer to provide support.

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