Identification box for comparing plant root growth

Abstract

The invention discloses an identification box for comparing the growth amount of a plant root system. The main purpose is to directly cut a root system growing from plant seeds below a culture net in a culture box. The identification box for comparing the plant root growth amount comprises a standard culture box, an identification box and a control system, wherein the standard culture box is used for storing a basic culture solution; the differential incubator is used for storing a variable culture solution; the joint culture cover is movably arranged at the open positions of the top ends of the standard culture box and the differential culture box, a plurality of culture openings are formed in the joint culture cover, culture nets are arranged in the culture openings, and the culture nets are used for bearing plant seeds; the cutting assembly comprises a rotating shaft and a cutting blade, the rotating shaft is rotatably arranged between the standard culture box and the differential culture box, the cutting blade is fixedly arranged on the peripheral surface of the rotating shaft, and under the condition that the rotating shaft drives the cutting blade to rotate, the cutting blade can cut root systems grown by plant seeds below the culture net.

Description

Technical Field

[0001] The present application belongs to the technical field of salt tolerance identification, and specifically relates to an identification box for comparing the growth of plant roots. Background Art

[0002] In the field of plant science research, especially in the research involving the identification of plant salt tolerance, accurately measuring and comparing the growth of plant roots is an extremely critical task. Plant roots are important organs for plants to absorb water and nutrients and perceive changes in the soil environment. Their growth status directly reflects the plant's ability to adapt to environmental stress (such as salt stress).

[0003] In traditional plant root research, obtaining root samples is a relatively complicated process. Researchers need to carefully remove plants from the culture solution to avoid damaging the roots. However, even so, conventional root sampling methods, such as manual digging or pulling plants out of the culture solution, will inevitably cause damage to the roots such as breakage and twisting. These damages caused by sampling will have many adverse effects on the subsequent accurate measurement of root growth. Once the root system is damaged, part of the root tissue may be separated from the main body, resulting in missing measurement data, and the internal physiological state of the root system will also change after the root system is damaged. For example, the osmotic pressure balance of the cells in the damaged area is broken, and the nutrient absorption and transport functions are blocked. These physiological changes will make the measurement results of the root growth unable to accurately reflect its true state in an undisturbed natural growth environment, thereby reducing the accuracy and reliability of the entire study. Summary of the invention

[0004] In view of this, the present application provides an identification box for comparing the growth of plant roots. The main purpose is to directly cut the roots grown from plant seeds under the culture net in the culture box, reducing the possibility of damage to the roots due to the extraction process and ensuring the integrity of the root samples.

[0005] In order to achieve the above objectives, this application mainly provides the following technical solutions:

[0006] The present application provides an identification box for comparing the growth of plant roots, comprising:

[0007] A standard incubator, wherein the standard incubator is used to store a basic culture solution;

[0008] A differential incubator, wherein the differential incubator is used to store variable culture fluid;

[0009] A combined culture cover, the combined culture cover is movably arranged at the top opening of the standard culture box and the differential culture box, the combined culture cover is provided with a plurality of culture ports, and a culture net is arranged in each of the plurality of culture ports, and the culture net is used to carry plant seeds;

[0010] The cutting assembly includes a rotating shaft and a cutting blade. The rotating shaft is rotatably disposed between the standard culture box and the differential culture box. The cutting blade is fixedly disposed on the outer peripheral surface of the rotating shaft. When the rotating shaft drives the cutting blade to rotate, the cutting blade can cut the roots grown from the plant seeds under the culture net.

[0011] Optionally, the number of culture ports provided on the combined culture cover relative to the standard culture box is the same as the number of culture ports provided on the combined culture cover relative to the differential culture box.

[0012] Optionally, a scraper is provided on the side of the cutting blade facing away from the combined cultivation cover, and the scraper moves synchronously with the cutting blade. After the rotating shaft drives the cutting blade to cut the roots grown from the plant seeds under the cultivation net, the scraper can peel off the cut roots.

[0013] Optionally, a side of the cutting blade facing away from the co-cultivation cover is further provided with a surrounding plate, a collecting basket and a collecting plate, the width of the surrounding plate is smaller than the width of the scraper, and the surrounding plate is arranged at an angle to the scraper, for constructing the side wall of the collecting chamber, the collecting basket is arranged at the bottom of the surrounding plate and the scraper, for constructing the bottom wall of the collecting chamber, the collecting plate is rotatably arranged on the collecting basket, and when the collecting plate is rotated in a direction close to the surrounding plate, the scraper, the surrounding plate, the collecting basket and the collecting plate can enclose the collecting chamber, and the collecting chamber is used to collect the roots grown from the germinated plant seeds at the position corresponding to the ungerminated plant seeds during the comparative experiment.

[0014] Optionally, the identification box for comparing the growth of plant roots further comprises a rotating connection member and an elastic driving member, the collection plate is rotatably connected to the collection basket via the rotating connection member, the elastic driving member is arranged at the rotating shaft of the rotating connection member, the first end of the elastic driving member is connected to the collection plate, and the second end of the elastic driving member is relatively fixed to the main body of the collection basket;

[0015] Wherein, when the collecting plate rotates toward the direction approaching the enclosure, the elastic driving member accumulates elastic potential energy; when the collecting plate rotates toward the direction away from the enclosure, the elastic potential energy of the elastic driving member is released.

[0016] Optionally, the identification box for comparing the growth of plant roots also includes a traction rope, which is connected to the top of the collecting plate, and the traction rope is used to apply traction to the collecting plate to drive the collecting plate to rotate in a direction close to the enclosure.

[0017] Optionally, a rotating rod is provided at the top of the rotating shaft, the rotating rod is perpendicular to the rotating shaft and fixed relative to the rotating shaft, a shift ring is provided on the side of the rotating rod close to the joint culture cover, the shift ring can be raised and lowered on the outer peripheral surface of the rotating shaft, and the end of the traction rope away from the collection plate is connected to the shift ring.

[0018] Optionally, a guide cylinder is provided between the standard incubator and the differential incubator, a first drain port communicating with the interior of the standard incubator is provided at a position of the bottom of the guide cylinder relative to the standard incubator, and a second drain port communicating with the interior of the differential incubator is provided at a position of the bottom of the guide cylinder relative to the differential incubator;

[0019] The rotating shaft can be raised and lowered in the guide cylinder, and a water stop plug is provided at the bottom of the rotating shaft. The rotating shaft is used to drive the water stop plug to switch between a first position and a second position. When the water stop plug is switched to the first position, the outer peripheral surface of the water stop plug seals the first drain port and the second drain port. When the water stop plug is switched to the second position, the outer peripheral surface of the water stop plug is out of contact with the first drain port and the second drain port.

[0020] Optionally, the identification box for comparing the growth of plant roots also includes an air supply device, the rotating shaft is a hollow structure to form an air duct, the air supply device is connected to the air duct, and a plurality of air outlets are provided at the bottom of the air duct. When the water stop plug is switched to the second position, the plurality of air outlets are respectively in a relative position relationship with the first drain outlet and the second drain outlet along the radial direction of the rotating shaft.

[0021] Optionally, a support ring is provided on the outer peripheral surface of the standard incubator and the differential incubator, a support frame is clamped at the bottom of the support ring, and a support leg is fixedly connected to the bottom of the support frame.

[0022] By means of the above technical solution, the present application has at least the following beneficial effects:

[0023] The identification box for comparing the growth of plant roots provided in the embodiments of the present application can create a standard environment and a variable environment in the same device to cultivate plant seeds by setting a standard culture box and a differential culture box, thereby avoiding environmental differences caused by factors such as different experimental devices and different experimental batches, and can more accurately compare the growth differences of plant roots under different conditions, providing a reliable data basis for studying the response mechanism of plants to specific factors. At the same time, by setting a cutting component, the roots under the culture net can be cut and sampled directly in the culture box, so that when the roots grow to a specific stage, samples can be obtained in time without taking the plants out of the culture solution for reprocessing. Furthermore, in-situ cutting can minimize the displacement, damage and environmental changes of the roots during the sampling process, ensuring that the samples taken can truly reflect their growth status in the culture environment, thereby improving the accuracy of the root growth measurement, and facilitating accurate comparison of the subtle differences in root growth under different culture conditions. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the three-dimensional structure of an identification box for comparing the growth of plant roots in an optional embodiment of the present application;

[0025] Figure 2 This is a schematic structural diagram of a cutting assembly of an optional embodiment of the present application;

[0026] Figure 3 A cross-sectional view of an identification box for comparing plant root growth according to an optional embodiment of the present application.

[0027] The reference numerals are:

[0028] 1. Standard incubator; 2. Differential incubator; 3. Combined incubation cover; 31. Incubation port; 32. Incubation net; 4. Cutting assembly; 41. Rotating axis; 411. Air duct; 412. Air outlet; 42. Cutting blade; 5. Scraper; 6. Enclosure; 7. Collection basket; 8. Collection plate; 9. Traction rope; 10. Rotating rod; 11. Dial ring; 12. First drain outlet; 13. Second drain outlet; 14. Water stopper; 15. Support ring; 16. Support frame; 17. Support leg. DETAILED DESCRIPTION

[0029] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0030] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0031] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0032] The preferred embodiments of the present application are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present application and are not used to limit the present application.

[0033] See also Figures 1 to 3 As shown, according to an embodiment of the present application, an identification box for comparing the growth of plant roots is provided, including: a standard culture box 1, the standard culture box 1 is used to store basic culture solution; a differential culture box 2, the differential culture box 2 is used to store variable culture solution; a combined culture cover 3, the combined culture cover 3 is movably arranged at the top opening of the standard culture box 1 and the differential culture box 2, and a plurality of culture ports 31 are opened on the combined culture cover 3, and a culture net 32 ​​is arranged in each of the plurality of culture ports 31, and the culture net 32 ​​is used to carry plant seeds; a cutting assembly 4, the cutting assembly 4 includes a rotating shaft 41 and a cutting blade 42, the rotating shaft 41 is rotatably arranged between the standard culture box 1 and the differential culture box 2, and the cutting blade 42 is fixedly arranged on the outer peripheral surface of the rotating shaft 41, and when the rotating shaft 41 drives the cutting blade 42 to rotate, the cutting blade 42 can cut the roots grown from the plant seeds below the culture net 32.

[0034] In this embodiment, by setting up a standard culture box 1 and a differential culture box 2, a standard environment and a variable environment can be created in the same device to cultivate plant seeds, avoiding environmental differences caused by different experimental devices, different experimental batches and other factors, and being able to more accurately compare the growth differences of plant roots under different conditions, providing a reliable data basis for studying the response mechanism of plants to specific factors. At the same time, by setting up a cutting component 4, the roots under the culture net 32 ​​can be cut and sampled directly in the culture box, so that when the roots grow to a specific stage, samples can be obtained in time without having to remove the plants from the culture solution for further processing. Furthermore, in-situ cutting can minimize the displacement, damage and environmental changes of the roots during the sampling process, ensuring that the samples taken can truly reflect their growth status in the culture environment, thereby improving the accuracy of the root growth measurement, and facilitating the accurate comparison of the subtle differences in root growth under different culture conditions.

[0035] Among them, the identification box for comparing the amount of plant root growth provided in this embodiment can be applied to plant salt tolerance research, plant nutrient element requirement research, plant growth regulator effect research, plant variety screening and identification, etc., and the present invention is not limited to this. In this embodiment, the identification box for comparing the amount of plant root growth is applied to plant salt tolerance research.

[0036] The identification box for comparing the growth of plant roots includes a standard incubator 1 and a differential incubator 2, both of which can be semi-cylindrical in shape. In practical applications, the two flat surfaces of the semi-cylindrical standard incubator 1 and the differential incubator 2 are tightly connected.

[0037] Among them, the standard incubator 1 is used to store the basic culture solution, which can be prepared according to conventional experimental requirements and standard formulas, and contains a combination of basic nutrient elements required for plant root growth, so as to provide a nutrient basis for plant root growth under a standard environment. The differential incubator 2 is used to store the variable culture solution, which has at least one component or characteristic different from the basic culture solution. The difference is a specially set comparison variable, which is used to create a comparison condition different from the plant root growth environment in the standard incubator 1, so as to realize the comparative measurement of the plant root growth amount under different culture solution environments.

[0038] Specifically, for example, when the identification box used to compare the amount of plant root growth is used to compare the adaptability of rice seeds to salt stress in different growth environments, rice seeds of the same type and similar quality and vitality are placed in the culture net 32 ​​of the joint culture cover 3 on the standard culture box 1 and the differential culture box 2. The basic culture solution in the standard culture box 1 maintains the ideal nutrition and environmental parameters for normal germination and root growth of rice seeds, and contains no or only very low concentrations of salt, which serves as a control benchmark. The variable culture solution in the differential culture box 2 is added with a specific concentration of salt to simulate the salt stress scenario.

[0039] Among them, when the standard incubator 1 and the differential incubator 2 are semi-cylindrical, the joint incubator cover 3 is circular. In practical applications, the inner diameter of the joint incubator cover 3 can be slightly larger than the outer diameter of the cylindrical whole formed by the two flat surfaces of the standard incubator 1 and the differential incubator 2 being tightly connected, so that the joint incubator cover 3 can be easily buckled at the top opening of the standard incubator 1 and the differential incubator 2, and has a certain operating margin during the installation and removal process, which is convenient for researchers to operate.

[0040] Specifically, a plurality of culture ports 31 are provided on the joint culture cover 3, and the number, size and spacing of the culture ports 31 can be designed according to the experimental requirements and the plant growth characteristics, and the present invention does not limit this. A culture net 32 ​​is provided in each culture port 31, and the material of the culture net 32 ​​can be a material that is non-toxic to the plant root system and has a certain mechanical strength, such as a nylon net or a stainless steel net. And the aperture size of the culture net 32 ​​is moderate, which can not only ensure that the plant seeds are stably placed on it, and will not be displaced due to the flow of the culture solution, but also allow the plant roots to smoothly pass through the mesh and grow downward into the culture solution. During the experiment, the joint culture cover 3 can provide a relatively stable placement platform for the plant seeds, while preventing pollutants such as dust and microorganisms from entering the culture box to affect the plant growth and experimental results. At the same time, the joint culture cover 3 is arranged in a movable manner so that when the inside of the culture box needs to be operated (such as adding culture solution, cleaning, etc.), it can be easily opened or removed, which improves the convenience of experimental operation.

[0041] The identification box for comparing the growth of plant roots also includes a cutting assembly 4, which includes a rotating shaft 41 and a cutting blade 42. The rotating shaft 41 is rotatably mounted at the intersection of the two flat surfaces of the standard culture box 1 and the differential culture box 2, and its rotation can be achieved by manual operation or by means of an electric device, which is not limited by the present invention. The cutting blade 42 is fixedly arranged on the outer peripheral surface of the rotating shaft 41, and the cutting blade 42 can be supported by stainless steel to ensure that the roots grown by the plant seeds under the culture net 32 ​​can be cleanly cut under the drive of the rotating shaft 41.

[0042] Specifically, the cutting assembly 4 can be equipped with two cutting blades 42, and the two cutting blades 42 are arranged relative to each other in the radial direction of the rotating shaft 41. In practical applications, when the plant root system grows to a preset measurement stage, the rotating shaft 41 is rotated 180 degrees by means of a manual or electric drive device. In this process, one of the cutting blades 42 accurately cuts the plant roots located under the culture net 32 ​​in the standard incubator 1 by virtue of a specific rotation speed and force; at the same time, the other cutting blade 42 simultaneously cuts the roots in the differential incubator 2. It should be noted that since the entire cutting process is carried out directly in situ inside the incubator, the plant roots do not need to go through the cumbersome and fragile process of being taken out of the culture solution and transferred to the external environment. In this way, the displacement of the root system caused by external force pulling, transportation and other operations is avoided to the greatest extent, and mechanical damage such as breakage and twisting of the root system due to uneven force is effectively prevented. Moreover, the roots are always in a stable culture solution environment, successfully avoiding adverse conditions such as water loss, osmotic pressure imbalance, and other changes in physiological state caused by separation from the culture solution. Based on this, the collected root samples can accurately and faithfully present their growth characteristics in the original culture environment, laying a solid and reliable sample foundation for the subsequent precise comparative analysis of root growth differences under different culture conditions, and effectively promoting the scientificity and accuracy of plant root growth research.

[0043] In some possible implementations disclosed in this application, see Figure 1 As shown, the number of culture ports 31 arranged on the combined culture cover 3 relative to the standard culture box 1 is the same as the number of culture ports 31 arranged on the combined culture cover 3 relative to the differential culture box 2 .

[0044] In this embodiment, the same number of culture ports 31 ensures that the same number of plant seeds participate in the experiment in the standard culture box 1 and the differential culture box 2. Therefore, when analyzing the data later, the two groups of experimental results can be compared more evenly, avoiding experimental deviation caused by the difference in sample quantity, so that researchers can more accurately evaluate the effect of variables (such as salt concentration) on the growth of plant roots.

[0045] Specifically, the culture port 31 provided on the joint culture cover 3 relative to the standard culture box 1 and the culture port 31 provided on the joint culture cover 3 relative to the differential culture box 2 are arranged symmetrically, and the center of symmetry may be the axis of the rotation axis 41. It should be noted that the symmetrically arranged culture ports 31 facilitate researchers to directly compare the germination and root growth of plant seeds in the same position but under different culture environments. At the same time, it is also convenient to remove the roots of germinated and ungerminated seeds in symmetrical positions, which can effectively reduce experimental errors.

[0046] In some possible implementations disclosed in this application, see Figure 2 As shown, a scraper 5 is provided on the side of the cutting blade 42 away from the co-cultivation cover 3, and the scraper 5 moves synchronously with the cutting blade 42. After the rotating shaft 41 drives the cutting blade 42 to cut the roots grown by the plant seeds under the cultivation net 32, the scraper 5 can peel off the cut roots.

[0047] In this embodiment, after cutting the root system, the scraper 5 can promptly peel off the cut root system to prevent the root system from remaining near the culture net 32, so that after each cutting operation, the root system sample can be quickly and completely collected, reducing the time for researchers to manually clean and collect root system samples. In other words, in large-scale experiments or when it is necessary to frequently obtain root system samples for analysis, the presence of the scraper 5 can significantly improve the efficiency of sample acquisition and make the experimental process smoother.

[0048] Specifically, the scraper 5 can be a vertical plate. In practical applications, when the experiment reaches the stage where plant root samples need to be obtained, that is, the plant seeds are in the standard culture box 1 or the differential culture box 2, and after growing in their respective culture solution environments, their roots have developed to a degree suitable for measurement and analysis under the culture net 32. At this time, the rotating shaft 41 starts to rotate manually or with the help of a corresponding driving device (such as a motor, etc.). During the rotation of the rotating shaft 41, the cutting blade 42 fixed on its outer peripheral surface is driven to rotate accordingly to cut the roots grown by the plant seeds under the culture net 32. Since the scraper 5 moves synchronously with the cutting blade 42, during the cutting action, the scraper 5 will move with the cutting blade 42, effectively peeling off the roots attached to the position of the culture net 32 ​​after cutting, ensuring that the cut roots can be completely and smoothly separated from the cutting area, so as to facilitate the subsequent collection and further analysis of the root samples.

[0049] In some possible implementations disclosed in this application, see Figure 2 As shown, a side of the cutting blade 42 facing away from the co-cultivation cover 3 is also provided with a surrounding plate 6, a collecting basket 7 and a collecting plate 8. The width of the surrounding plate 6 is smaller than the width of the scraper 5, and the surrounding plate 6 is arranged at an angle to the scraper 5 to construct the side wall of the collecting cavity. The collecting basket 7 is arranged at the bottom of the surrounding plate 6 and the scraper 5 to construct the bottom wall of the collecting cavity. The collecting plate 8 is rotatably arranged on the collecting basket 7. When the collecting plate 8 is rotated toward the direction close to the surrounding plate 6, the scraper 5, the surrounding plate 6, the collecting basket 7 and the collecting plate 8 can enclose a collecting cavity. The collecting cavity is used to collect roots grown from germinated plant seeds at positions corresponding to ungerminated plant seeds during the comparative experiment.

[0050] In this embodiment, through the synergistic effect of the enclosure 6, the scraper 5, the collection basket 7 and the collection plate 8, a collection chamber specifically used to collect the roots of germinated seeds at positions corresponding to the ungerminated seeds can be formed, which helps to accurately obtain the samples required for the comparative experiment and provides a more targeted data basis for the subsequent comparison of root growth differences under different conditions.

[0051] The enclosing plate 6 is located on the forward direction side of the scraper 5. In practical applications, when the rotating shaft 41 rotates to drive the cutting blade 42 to cut the root system, the cutting blade 42 will have a rotational movement direction. For the scraper 5 fixed to the side of the cutting blade 42 away from the co-cultivation cover 3, its forward direction is the direction in which it moves as the cutting blade 42 rotates.

[0052] Specifically, the width of the enclosure 6 is smaller than that of the scraper 5, which can ensure that the scraper 5 plays a major role in guiding and peeling off the roots during the movement, while the enclosure 6 serves as an auxiliary boundary, and the two work together to construct a reasonable collection space contour. At the same time, the enclosure 6 and the scraper 5 are set at an angle, so that the roots that need to be collected can smoothly move along the space area formed by the enclosure 6 and the scraper 5 to the inside of the collection chamber after being peeled off by the scraper 5. It can be understood that the enclosure 6 limits the range of the collection chamber from the side, so that the roots that need to be collected have clear boundary restrictions during the collection process, avoiding the roots that need to be collected from being scattered to irrelevant areas, thereby achieving the rapid removal of ungerminated seeds to avoid their impact on the experimental results. It should be noted that the roots that need to be collected are the roots of germinated seeds at the position corresponding to the ungerminated seeds. The root system of the germinated seeds at the position corresponding to the ungerminated seeds refers to the corresponding position in the standard culture box 1 and the differential culture box 2, for each position where the seeds are placed, in the same experimental environment (except for the difference in culture solution), if one seed does not germinate, but the other seed germinates, then the root system grown by the germinated seeds is what we need to collect. For example, with the axis of the rotating shaft 41 as the center of symmetry, the culture ports 31 of the standard culture box 1 and the differential culture box 2 on the joint culture cover 3 are arranged symmetrically. For a pair of seeds in symmetrical positions, if one side of the seeds does not germinate, but the other side of the seeds germinates, the root system of the germinated seeds corresponding to this symmetrical position is the root system that needs to be collected.

[0053] Among them, a collecting basket 7 is arranged at the bottom of the enclosure 6 and the scraper 5. The collecting basket 7 is arranged horizontally. The collecting basket 7 can serve as the bottom wall of the collecting chamber, providing a bottom support and accommodation space for collecting root samples, ensuring that the cut roots have a relatively concentrated placement area.

[0054] Among them, a collecting plate 8 is rotatably provided on the collecting basket 7, and the collecting plate 8 can rotate to adjust the specific shape and space size of the collecting cavity to better meet the needs of root collection in different situations. It should be noted that when the collecting plate 8 rotates in the direction close to the enclosure 6, the collecting plate 8 will work together with the scraper 5, the enclosure 6 and the collecting basket 7 that are already in a specific position. The scraper 5 is one of the boundaries, and since it is responsible for peeling off the roots after the cutting blade 42 cuts the roots, the scraper 5, the enclosure 6, the collecting basket 7 and the rotating collecting plate 8 form a relatively closed space, namely the collecting cavity. The side wall of the collecting cavity is mainly composed of the enclosure 6 and the scraper 5, and the bottom wall is borne by the collecting basket 7. After the collecting plate 8 is adjusted to a suitable position by rotation, the boundary of one side of the collecting cavity is improved, so that the entire collecting cavity can effectively collect the cut roots that need to be collected.

[0055] Specifically, in practical applications, when all plant seeds on the co-cultivation cover 3 germinate successfully, there are no roots that meet the specific collection requirements (i.e., roots grown from germinated seeds at positions corresponding to ungerminated seeds). At this time, the scraper 5 cooperates with the enclosure 6, the collection basket 7, and the collection plate 8 rotated to the corresponding position to jointly construct a relatively closed space structure. However, when ungerminated plant seeds appear on one side of the co-cultivation cover 3 at a certain symmetrical position, the collection chamber remains relatively closed before the scraper 5 moves to the germinated seed root corresponding to the symmetrical position. Once the scraper 5 moves to the symmetrical position, the collection plate 8 immediately rotates in a direction away from the enclosure 6, thereby opening the collection chamber. Next, the cutting blade 42 performs a cutting operation on the root system that needs to be collected, and then the collection plate 8 quickly rotates in a direction close to the enclosure 6, so as to push the cut, qualified root system into the collection chamber, and close the collection chamber again, ensuring that the collected root system samples are complete and in a specific collection area, so as to facilitate subsequent accurate experimental analysis and data processing.

[0056] In some possible embodiments disclosed in the present application, the identification box for comparing the growth of plant roots also includes a rotating connector and an elastic driving member. The collecting plate 8 is rotatably connected to the collecting basket 7 via the rotating connector. The elastic driving member is arranged at the rotating shaft of the rotating connector. The first end of the elastic driving member is connected to the collecting plate 8, and the second end of the elastic driving member is relatively fixed to the main body of the collecting basket 7. When the collecting plate 8 rotates toward the direction approaching the enclosure 6, the elastic driving member accumulates elastic potential energy. When the collecting plate 8 rotates toward the direction away from the enclosure 6, the elastic potential energy of the elastic driving member is released.

[0057] In this embodiment, by providing an elastic driving member, the collecting plate 8 can automatically open the collecting chamber at an appropriate time. Therefore, when cutting the root system of the germinated seeds at the position corresponding to the ungerminated seeds, it is not necessary to manually open the collecting chamber, which facilitates the cutting blade 42 to smoothly perform the cutting operation.

[0058] The rotating connector may be a rotating shaft for realizing the rotational connection between the collecting plate 8 and the collecting basket 7, so that the collecting plate 8 can rotate relative to the collecting basket 7, thereby changing the opening state of the collecting chamber.

[0059] The elastic driving member may be a torsion spring, which is arranged at the rotating shaft of the rotating connecting member, and whose two ends are respectively connected to the collecting plate 8 and the main body of the collecting basket 7.

[0060] Specifically, in actual application, when the collecting plate 8 rotates in a direction close to the enclosure 6, the collecting plate 8 will squeeze the elastic driving member. Taking the torsion spring as an example, the torsion spring is compressed at this time, and the elastic potential energy will be accumulated inside the torsion spring. On the contrary, when the collecting plate 8 rotates in a direction away from the enclosure 6, the elastic potential energy accumulated by the elastic driving member will be released. For example, for the above-mentioned torsion spring, the torsion spring will return to its original state at this time, converting the elastic potential energy stored previously into mechanical energy, pushing the collecting plate 8 to rotate, so that the collecting plate 8 can automatically open the collecting chamber.

[0061] In the above embodiment, the identification box for comparing the growth of plant roots also includes a traction rope 9, which is connected to the top of the collecting plate 8. The traction rope 9 is used to apply traction to the collecting plate 8 to drive the collecting plate 8 to rotate toward the direction close to the enclosure 6.

[0062] In this embodiment, a traction rope 9 is provided to provide a driving method for the collection plate 8 to rotate toward the direction close to the enclosure 6. In practical applications, by applying traction force through the traction rope 9, the rotation angle and rotation timing of the collection plate 8 can be accurately controlled. For example, when the collection chamber needs to be closed to preserve the cut root system, the researchers can operate the traction rope 9 to rotate the collection plate 8 slowly or quickly according to actual needs to ensure that the collection chamber can be closed in time and accurately, so as to better collect and preserve the root system.

[0063] Specifically, the traction force acts in the direction of driving the collecting plate 8 to rotate toward the direction close to the enclosure 6. In the structure of the collecting chamber mentioned above, the enclosure 6 is a part of the side wall of the collecting chamber. When the collecting plate 8 rotates toward the direction close to the enclosure 6, it works together with the scraper 5, the enclosure 6 and the collecting basket 7 to form a relatively closed collecting chamber.

[0064] In some possible implementations disclosed in this application, see Figure 1As shown, a rotating rod 10 is arranged on the top of the rotating shaft 41, the rotating rod 10 is perpendicular to the rotating shaft 41 and is fixed relative to the rotating shaft 41, a shifting ring 11 is arranged on the side of the rotating rod 10 close to the joint culture cover 3, the shifting ring 11 can be raised and lowered on the outer peripheral surface of the rotating shaft 41, and the end of the traction rope 9 away from the collecting plate 8 is connected to the shifting ring 11.

[0065] In this embodiment, by connecting the traction rope 9 with the dial ring 11, and the dial ring 11 is arranged on the side of the rotating rod 10 close to the joint culture cover 3 and can be lifted and lowered on the outer peripheral surface of the rotating shaft 41, when an external force is applied to the dial ring 11 to make it lift and lower, this movement can be effectively transmitted to the collection plate 8 through the traction rope 9. Thus, a force transmission path from the dial ring 11 to the collection plate 8 is established, so that the force applied to the dial ring 11 from the outside can be accurately converted into a traction force to drive the collection plate 8 to rotate, thereby realizing the control of the rotation of the collection plate 8, and then affecting the opening and closing state of the collection chamber. Furthermore, by setting the dial ring 11 to be liftable, a more intuitive and convenient operation method is provided for researchers. Researchers do not need to directly contact the collection plate 8 or the more complex structure near it to control the rotation of the collection plate 8, and only need to lift and lower the dial ring 11 near the top of the rotating shaft 41. For example, when it is necessary to close the collection chamber to collect the roots that need to be collected, researchers can easily press or pull up the dial ring 11 to transmit the corresponding traction force to the collection plate 8 via the traction rope 9, so that it rotates toward the direction close to the enclosure 6, thereby completing the closing operation of the collection chamber, which is easier than operating directly on the collection plate 8.

[0066] The traction rope 9 can be closely attached to the scraper 5 and passed through the rotating shaft 41 to be connected to the dial ring 11, thereby reducing the possibility of interference between the traction rope 9 and other running components.

[0067] Among them, the rotating rod 10 is provided on the side of the dial ring 11 away from the joint culture cover 3, that is, on the top of the rotating rod 10. The rotating rod 10 is perpendicular to the rotating shaft 41 and fixed relative to it. When the rotating shaft 41 is rotated, the rotating rod 10 is equivalent to a force arm. When the rotating shaft 41 needs to be rotated manually, by applying force on the rotating rod 10, according to the lever principle, the rotation of the rotating shaft 41 can be achieved more effortlessly. At the same time, the existence of the rotating rod 10 makes the operation of the dial ring 11 more convenient. When operating the dial ring 11, the researcher will use the rotating rod 10 as a reference, put the palm on the rotating rod 10, and then gently pinch the dial ring 11 with the thumb and index finger, and determine the initial position and lifting direction of the dial ring 11 according to the position of the rotating rod 10. For example, when the dial ring 11 needs to be lowered, the fingers apply downward pressure to make the dial ring 11 smoothly descend along the outer peripheral surface of the rotating shaft 41.

[0068] In some possible implementations disclosed in this application, see Figure 3As shown, a guide cylinder is arranged between the standard incubator 1 and the differential incubator 2, a first drain port 12 communicating with the interior of the standard incubator 1 is provided at the bottom of the guide cylinder relative to the position of the standard incubator 1, and a second drain port 13 communicating with the interior of the differential incubator 2 is provided at the bottom of the guide cylinder relative to the position of the differential incubator 2; a rotating shaft 41 is arranged in the guide cylinder so as to be liftable, a water stop plug 14 is arranged at the bottom of the rotating shaft 41, and the rotating shaft 41 is used to drive the water stop plug 14 to switch between a first position and a second position, when the water stop plug 14 is switched to the first position, the outer peripheral surface of the water stop plug 14 seals the first drain port 12 and the second drain port 13, and when the water stop plug 14 is switched to the second position, the outer peripheral surface of the water stop plug 14 is out of contact with the first drain port 12 and the second drain port 13.

[0069] In this embodiment, by setting a guide cylinder, and opening a first drain port 12 and a second drain port 13 at the bottom thereof relative to the standard incubator 1 and the differential incubator 2, respectively, and using a rotating shaft 41 to drive the water stop plug 14 to switch between different positions, precise control of drainage of the culture solution in the standard incubator 1 and the differential incubator 2 is achieved. When the water stop plug 14 is switched to the first position, its outer peripheral surface seals the first drain port 12 and the second drain port 13, and at this time, the culture solution in the two incubators is retained, ensuring that the plant roots have sufficient growth environment in the culture solution, and avoiding premature loss of the culture solution that affects the growth of the plant roots. When drainage is required, such as replacing the culture solution or performing other related operations, the water stop plug 14 is switched to the second position, so that the outer peripheral surface of the water stop plug 14 is out of contact with the first drain port 12 and the second drain port 13, so that the culture solution in the incubator can be smoothly discharged, and the operation is convenient and the drainage control is precise.

[0070] The guide cylinder is used to guide and support the rotating shaft 41 , and the rotating shaft 41 is rotatably and liftably arranged in the guide cylinder.

[0071] Specifically, in some specific examples, semi-cylindrical grooves are processed on the two flat surfaces of the standard incubator 1 and the differential incubator 2. When the flat surfaces of the two incubators are tightly fitted and docked, the two semi-cylindrical grooves are just spliced ​​and combined, so that a guide cylinder structure is naturally formed between the standard incubator 1 and the differential incubator 2. In other specific examples, an independent guide cylinder component is separately placed between the standard incubator 1 and the differential incubator 2. This guide cylinder component has a standard cylindrical appearance. As an independent functional component in the entire device system, it cooperates with the standard incubator 1 and the differential incubator 2 to jointly build a complete drainage and related functional operation system.

[0072] A first drain port 12 is provided at the bottom of the guide cylinder at a position relative to the standard culture box 1. The first drain port 12 is used to connect to the inside of the standard culture box 1, so that the basic culture solution in the standard culture box 1 can flow out through the first drain port 12 when certain conditions (such as when drainage is required) are met. Similarly, a second drain port 13 is provided at the bottom of the guide cylinder at a position relative to the differential culture box 2. The second drain port 13 is used to connect to the inside of the differential culture box 2, providing a discharge channel for the variable culture solution in the differential culture box 2.

[0073] The rotating shaft 41 is arranged in the guide cylinder in a manner that it can be raised and lowered, which means that the rotating shaft 41 is not fixed, but can move up and down along the vertical direction defined by the guide cylinder. At the same time, the rotating shaft 41 may also have a rotating function (for example, when performing a root cutting operation, the rotating shaft 41 drives the cutting blade 42 to rotate). This lifting and rotating feature enables the rotating shaft 41 to realize multiple functions in the entire device.

[0074] A water stopper 14 is provided at the bottom of the rotating shaft 41, and the water stopper 14 is closely connected to the rotating shaft 41, and the two form a linked whole. In other words, the movement of the rotating shaft 41 can directly drive the water stopper 14 to perform corresponding actions, and the precise control of the position of the water stopper 14 is achieved through this connection method.

[0075] Specifically, when the water stopper 14 is switched to the first position under the drive of the rotating shaft 41, the outer peripheral surface of the water stopper 14 can seal the first drain port 12 and the second drain port 13. Here, "sealing" means that the shape, size, material and other characteristics of the water stopper 14 enable it to fit tightly with the edges of the first drain port 12 and the second drain port 13 to form an effective closed state, just like plugging both drain ports with tight plugs. In this state, the culture solution in the standard incubator 1 and the differential incubator 2 cannot flow out through the two drain ports, thereby ensuring that the culture solution can be retained in their respective incubators, providing a continuous and stable growth environment for the plant roots, and avoiding the adverse effects on the growth of the plant roots due to the premature loss of the culture solution. When the water stopper 14 is switched to the second position, the outer peripheral surface of the water stopper 14 is out of contact with the first drain port 12 and the second drain port 13. At this time, the first drain port 12 and the second drain port 13 are no longer blocked by the water stopper 14 and are in an open state. In this way, the culture fluid in the standard incubator 1 and the difference incubator 2 can flow smoothly into the guide cylinder through their corresponding drainage ports, and then can be discharged to the outside of the identification box through other devices such as drainage pipes that may be set up later, thereby realizing the drainage operation of the culture fluid in the incubator.

[0076] In some possible implementations disclosed in this application, see Figure 3As shown, the identification box for comparing the growth of plant roots also includes an air supply device, the rotating shaft 41 is a hollow structure to form an air duct 411, the air supply device is connected to the air duct 411, and a plurality of air outlet holes 412 are provided at the bottom of the air duct 411. When the water stopper 14 is switched to the second position, the plurality of air outlet holes 412 are respectively in a relative position relationship with the first drain outlet 12 and the second drain outlet 13 along the radial direction of the rotating shaft 41.

[0077] In this embodiment, when the water stopper 14 is switched to the second position, the plurality of air outlets 412 are in relative positional relationship with the first drain port 12 and the second drain port 13 respectively along the radial direction of the rotating shaft 41. At this time, the gas delivery device delivers gas to the hollow rotating shaft 41, and the gas is ejected from the plurality of air outlets 412 at the bottom of the air duct 411. Since the air outlet 412 is opposite to the drain port, the ejected gas can directly act on the area near the drain port. After drainage, some moisture will remain on the surface of the plant root system. The flow of these gases will form strong air convection around the root system, directly blowing on the root surface to improve the evaporation efficiency and dissipate the moisture on the root surface faster, which is conducive to the subsequent accurate measurement of relevant indicators such as the root growth amount, and avoids the influence of excess moisture on the accuracy of the measurement results.

[0078] Specifically, the gas delivery device can be an air pump, etc., and its output end establishes a stable connection relationship with the top of the air duct 411 formed by the rotating shaft 41. In practical applications, when the air pump is started, the gas generated by the air pump flows smoothly into the air duct 411 under pressure. The gas continues to be conducted downward along the hollow channel inside the air duct 411, and finally reaches the bottom of the air duct 411. Since the multiple air outlets 412 opened at the bottom of the air duct 411 are in a precise relative position relationship with the first drain port 12 and the second drain port 13 along the radial direction of the rotating shaft 41 when the water stopper 14 is switched to the second position, the gas can be ejected from these air outlets 412 in a specific direction, and blown straight up from the bottom of the incubator after drainage. In this way, the gas can fully act on the surface of the rice root system, and the rapid evaporation of the water on the surface of the rice root is achieved by means of the rapid flow of the air flow and the physical processes such as heat exchange and kinetic energy transfer between the gas and the water on the surface of the root system.

[0079] In some possible implementations disclosed in this application, see Figure 1 As shown, a support ring 15 is provided on the outer peripheral surface of the standard incubator 1 and the differential incubator 2 , a support frame 16 is clamped at the bottom of the support ring 15 , and a support leg 17 is fixedly connected to the bottom of the support frame 16 .

[0080] In this embodiment, by providing the support ring 15, the support frame 16 and the support leg 17, the weight of the incubator can be effectively borne, the shaking of the device can be reduced, and the incubator can be prevented from tipping over due to external force, thereby ensuring the safety of the experiment and the stability of the device.

[0081] The support ring 15 is arranged on the outer peripheral surface of the standard incubator 1 and the differential incubator 2, providing an annular support base for the entire incubator.

[0082] The support frame 16 is connected to the support ring 15 by a snap-fitting manner, so that the support frame 16 can effectively bear the weight of the incubator.

[0083] The support legs 17 are fixed to the bottom of the support frame 16 to further distribute the weight of the entire device to the ground or the placement platform.

[0084] It is easy for those skilled in the art to understand that, under the premise of no conflict, the above-mentioned advantageous methods can be freely combined and superimposed.

[0085] The above are only preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present application shall be included in the protection scope of the present application. The above are only preferred implementations of the present application. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present application, and these improvements and variations should also be regarded as the protection scope of the present application.

Claims

1. An identification box for comparing the growth of plant roots, characterized in that: include: A standard incubator (1), wherein the standard incubator (1) is used to store a basic culture solution; A differential culture box (2), wherein the differential culture box (2) is used to store variable culture fluid; A combined culture cover (3), the combined culture cover (3) being movably arranged at the top openings of the standard culture box (1) and the differential culture box (2), the combined culture cover (3) being provided with a plurality of culture ports (31), each of the plurality of culture ports (31) being provided with a culture net (32), the culture net (32) being used for carrying plant seeds; A cutting assembly (4), the cutting assembly (4) comprising a rotating shaft (41) and a cutting blade (42), the rotating shaft (41) being rotatably disposed between the standard culture box (1) and the differential culture box (2), the cutting blade (42) being fixedly disposed on the outer peripheral surface of the rotating shaft (41), and when the rotating shaft (41) drives the cutting blade (42) to rotate, the cutting blade (42) can cut the roots grown from the plant seeds under the culture net (32).

2. The identification box for comparing the growth of plant roots according to claim 1, characterized in that: The number of culture ports (31) arranged on the combined culture cover (3) relative to the position of the standard culture box (1) is the same as the number of culture ports (31) arranged on the combined culture cover (3) relative to the position of the differential culture box (2).

3. The identification box for comparing the growth of plant roots according to claim 2, characterized in that: A scraper (5) is provided on the side of the cutting blade (42) facing away from the combined cultivation cover (3); the scraper (5) moves synchronously with the cutting blade (42); after the rotating shaft (41) drives the cutting blade (42) to cut the roots grown from the plant seeds under the cultivation net (32), the scraper (5) can peel off the cut roots.

4. The identification box for comparing the growth of plant roots according to claim 3, characterized in that: A side of the cutting blade (42) facing away from the co-cultivation cover (3) is also provided with a surrounding plate (6), a collecting basket (7) and a collecting plate (8); the width of the surrounding plate (6) is smaller than the width of the scraper (5), and the surrounding plate (6) and the scraper (5) are arranged at an angle to construct the side wall of the collecting chamber; the collecting basket (7) is arranged at the bottom of the surrounding plate (6) and the scraper (5) to construct the bottom wall of the collecting chamber; the collecting plate (8) is rotatably arranged on the collecting basket (7); when the collecting plate (8) is rotated in a direction close to the surrounding plate (6), the scraper (5), the surrounding plate (6), the collecting basket (7) and the collecting plate (8) can enclose the collecting chamber, and the collecting chamber is used to collect the roots grown from the germinated plant seeds at the position corresponding to the ungerminated plant seeds during the comparative experiment.

5. The identification box for comparing the growth of plant roots according to claim 4, characterized in that: It also comprises a rotating connection member and an elastic driving member, the collecting plate (8) is rotatably connected to the collecting basket (7) via the rotating connection member, the elastic driving member is arranged at the rotating shaft of the rotating connection member, the first end of the elastic driving member is connected to the collecting plate (8), and the second end of the elastic driving member is relatively fixed to the main body of the collecting basket (7); Wherein, when the collecting plate (8) rotates in a direction approaching the enclosure (6), the elastic driving member accumulates elastic potential energy; when the collecting plate (8) rotates in a direction away from the enclosure (6), the elastic potential energy of the elastic driving member is released.

6. The identification box for comparing the growth of plant roots according to claim 5, characterized in that: It also includes a traction rope (9), which is connected to the top of the collecting plate (8), and the traction rope (9) is used to apply traction to the collecting plate (8) to drive the collecting plate (8) to rotate in a direction close to the enclosure plate (6).

7. The identification box for comparing the growth of plant roots according to claim 6, characterized in that: A rotating rod (10) is arranged at the top of the rotating shaft (41), and the rotating rod (10) is perpendicular to the rotating shaft (41) and fixed relative to the rotating shaft (41). A shifting ring (11) is arranged on the side of the rotating rod (10) close to the joint culture cover (3), and the shifting ring (11) is arranged on the outer peripheral surface of the rotating shaft (41) in a liftable manner. The end of the traction rope (9) away from the collecting plate (8) is connected to the shifting ring (11).

8. The identification box for comparing the growth of plant roots according to claim 1, characterized in that: A guide cylinder is provided between the standard culture box (1) and the differential culture box (2); a first drainage port (12) communicating with the interior of the standard culture box (1) is provided at a bottom of the guide cylinder relative to the position of the standard culture box (1); and a second drainage port (13) communicating with the interior of the differential culture box (2) is provided at a bottom of the guide cylinder relative to the position of the differential culture box (2); The rotating shaft (41) is movably arranged in the guide cylinder. A water stop plug (14) is arranged at the bottom of the rotating shaft (41). The rotating shaft (41) is used to drive the water stop plug (14) to switch between a first position and a second position. When the water stop plug (14) is switched to the first position, the outer peripheral surface of the water stop plug (14) seals the first drain port (12) and the second drain port (13). When the water stop plug (14) is switched to the second position, the outer peripheral surface of the water stop plug (14) is out of contact with the first drain port (12) and the second drain port (13).

9. The identification box for comparing the growth of plant roots according to claim 8, characterized in that: The invention also comprises an air delivery device, wherein the rotating shaft (41) is a hollow structure to form an air duct (411), the air delivery device is connected to the air duct (411), a plurality of air outlet holes (412) are provided at the bottom of the air duct (411), and when the water stopper (14) is switched to the second position, the plurality of air outlet holes (412) are respectively in a relative positional relationship with the first drain port (12) and the second drain port (13) along the radial direction of the rotating shaft (41).

10. The identification box for comparing the growth of plant roots according to claim 1, characterized in that: The outer peripheral surfaces of the standard incubator (1) and the differential incubator (2) are provided with support rings (15), the bottom of the support ring (15) is clamped with a support frame (16), and the bottom of the support frame (16) is fixedly connected with support legs (17).

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