Composition with tightening and anti-wrinkle effects as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and discloses a composition with tightening and anti-wrinkle effects as well as a preparation method and application thereof. The composition disclosed by the invention comprises the following components: a radix pseudostellariae extract and hydrolyzed ginsenoside. The radix pseudostellariae extract in the composition achieves the effects of tightening and resisting wrinkles by promoting generation of collagen and elastin, the hydrolyzed ginsenoside mainly has the effects of resisting oxidation, inhibiting expression of matrix metalloproteinase and preventing collagen degradation, and after the radix pseudostellariae extract and the hydrolyzed ginsenoside are combined, the composition has a better effect of inhibiting the activity of the matrix metalloproteinase, so that the effect of resisting wrinkles is achieved. The traditional Chinese medicine composition has the effects of promoting the generation of collagen and elastin, and has a synergistic effect, so that the traditional Chinese medicine composition has an excellent anti-aging effect.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a composition with firming and anti-wrinkle effects, and a preparation method and application thereof. Background Art

[0002] Aging is the adverse changes that gradually occur in organ functions and body tissues as we age. As the largest and most obvious organ in the human body, the skin is the most obvious in the process of human aging. Skin aging is mainly determined by endogenous and exogenous factors. Its external characteristics are mainly manifested in reduced skin moisture content, dull skin color, pigmentation, dry and rough surface, loose skin, reduced elasticity, and wrinkle formation.

[0003] Traditional anti-aging cosmetics mostly contain retinol or its derivatives, peptides, collagen, etc. Although retinol or its derivatives have good effects, they are very irritating and can easily cause skin allergies, which is counterproductive; while peptides and collagen ingredients are generally unstable, easy to hydrolyze, and have poor permeability, making it difficult for these ingredients to exert their true effects.

[0004] Therefore, it is necessary to develop a mild, non-irritating, and effective anti-aging product. Summary of the invention

[0005] The present invention aims to solve at least one of the technical problems existing in the above-mentioned prior art. To this end, the present invention provides a composition with firming and anti-wrinkle effects, a preparation method and application thereof. The composition of the present invention is mild and non-irritating, has good effects of inhibiting the activity of matrix metalloproteinases, promoting the production of skin elastin and collagen type III, and has excellent anti-aging effects.

[0006] A first aspect of the present invention provides a composition comprising the following components: a Pseudostellaria heterophylla extract and hydrolyzed ginsenosides.

[0007] Specifically, the effects of each component in the composition of the present invention are as follows:

[0008] Pseudostellaria heterophylla, the dried root of Pseudostellaria heterophylla (Miq.) Pax ex Paxet Hoffm. of the Caryophyllaceae family, has the effects of invigorating qi and strengthening the spleen, promoting body fluid and moistening the lungs. It is commonly used for spleen deficiency, fatigue, loss of appetite, weakness after illness, insufficient qi and yin, spontaneous sweating and thirst, and dry cough due to lung dryness. The Pseudostellaria heterophylla extract of the present invention contains rich amino acids, cyclic peptides, sugars, trace elements and saponins, among which Pseudostellaria heterophylla cyclic peptide B is one of the main components of the traditional Chinese medicine Pseudostellaria heterophylla, and is also one of the most studied Pseudostellaria heterophylla cyclic peptide components. Pseudostellaria heterophylla cyclic peptide B in the Pseudostellaria heterophylla extract has the effects of promoting collagen and elastin production, scavenging free radicals, inhibiting MMPs, etc.

[0009] Hydrolyzed ginsenosides are rare ginsenosides. Traditional rare ginsenosides exist in wild ginseng over 100 years old, and the content is extremely low and the price is expensive. The hydrolyzed ginsenosides of the present invention are obtained by conventional biological enzymatic hydrolysis of common ginsenosides, the cost is controllable, and there is no waste of wild resources. It has good anti-inflammatory, anti-oxidation, and collagen production promotion effects.

[0010] In some embodiments of the present invention, the composition comprises the following components, measured in parts by mass: 0.001-10 parts of Pseudostellariae Radix extract and 0.001-10 parts of hydrolyzed ginsenosides.

[0011] In some embodiments of the present invention, the composition comprises the following components, measured in parts by mass: 0.01-10 parts of Pseudostellariae Radix extract and 0.01-10 parts of hydrolyzed ginsenosides.

[0012] In some embodiments of the present invention, the composition comprises the following components, measured in parts by mass: 0.1-10 parts of Pseudostellariae Radix extract and 0.1-10 parts of hydrolyzed ginsenosides.

[0013] In some embodiments of the present invention, the composition comprises the following components, measured by weight: 1-10 parts of Pseudostellariae Radix extract and 1-10 parts of hydrolyzed ginsenosides.

[0014] In some embodiments of the present invention, the composition further comprises a solvent and a solubilizer.

[0015] In some embodiments of the present invention, the solvent includes water and a polyol.

[0016] In some embodiments of the present invention, the polyol is added to the composition in an amount of 0.1-10 parts by mass.

[0017] In some embodiments of the present invention, the amount of the polyol added to the composition is 1-10 parts by mass.

[0018] In some embodiments of the present invention, the solubilizer is added to the composition in an amount of 0.1-10 parts by mass.

[0019] In some embodiments of the present invention, the solubilizer is added to the composition in an amount of 1-10 parts by mass.

[0020] In some embodiments of the present invention, the polyol includes at least one of 1,2-hexanediol, glycerol, propylene glycol, butylene glycol, 1,2-pentanediol, 1,3-propylene glycol and ethoxydiglycol.

[0021] In some embodiments of the present invention, the solubilizing agent includes at least one of lecithin, soybean seed extract, hydroxypropyl cyclodextrin, PEG-40 hydrogenated castor oil and PEG-60 hydrogenated castor oil.

[0022] The second aspect of the present invention provides a method for preparing the composition according to the first aspect of the present invention, comprising the following steps:

[0023] The components are stirred and mixed to obtain the composition.

[0024] In some embodiments of the present invention, the method for preparing the composition comprises the following steps:

[0025] The Pseudostellariae Radix extract and the hydrolyzed ginsenosides are dissolved in polyol at 40-45° C., and then a solubilizer and water are added thereto, and the mixture is stirred and mixed to obtain the composition.

[0026] According to a third aspect of the present invention, a cosmetic is provided, wherein the cosmetic comprises the composition according to the first aspect.

[0027] In some embodiments of the present invention, the content of the composition in the cosmetic is 0.01-20 wt %.

[0028] In some embodiments of the present invention, the content of the composition in the cosmetic is 0.1-10 wt %.

[0029] In some embodiments of the present invention, the dosage form of the cosmetic includes cream, lotion, water, gel or mask.

[0030] In some embodiments of the present invention, the cosmetic is a skin cream, which further comprises at least one of an emollient, an emulsifier, a moisturizer, a thickener and a preservative.

[0031] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0032] The composition provided by the present invention comprises a Pseudostellariae Radix extract and hydrolyzed ginsenosides; wherein the Pseudostellariae Radix extract achieves firming and anti-wrinkle effects by promoting the production of collagen and elastin, and the hydrolyzed ginsenosides mainly play the role of anti-oxidation, inhibiting the expression of matrix metalloproteinases, and preventing collagen degradation. The combination of the two has better effects of inhibiting the activity of matrix metalloproteinases and promoting the production of collagen and elastin, and plays a synergistic role, thereby having excellent anti-aging effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 The results of MMP-1 gene expression detection in Examples 1-4 and Comparative Examples 1-4;

[0034] Figure 2 The results of the Collagen III gene expression detection of Examples 1-4 and Comparative Examples 1-4;

[0035] Figure 3 These are the results of Elastin gene expression detection in Examples 1-4 and Comparative Examples 1-4. DETAILED DESCRIPTION

[0036] The present invention is further described in detail below by specific examples. The raw materials, reagents or devices used in the examples can be obtained from conventional commercial sources or by prior art methods unless otherwise specified. Unless otherwise specified, the experiments or test methods are conventional methods in the art.

[0037] Example 1

[0038] A composition comprising the following components in parts by weight:

[0039] 0.1 parts of Pseudostellariae Radix extract, 0.1 parts of hydrolyzed ginsenosides, 3 parts of 1,2-hexanediol, 3 parts of Glycine max seed extract, and the balance is deionized water.

[0040] The preparation method of the above-mentioned composition includes the following steps: at 45°C, mixing Pseudostellariae Radix extract and hydrolyzed ginsenosides in proportion, adding 1,2-hexanediol, and stirring to dissolve completely; then adding Glycine max seed extract and stirring thoroughly, and finally adding deionized water and stirring to mix evenly.

[0041] Example 2

[0042] A composition comprising the following components in parts by weight:

[0043] 0.01 parts of Pseudostellariae Radix extract, 0.01 parts of hydrolyzed ginsenosides, 3 parts of 1,2-hexanediol, 3 parts of wild soybean seed extract, and the balance is deionized water.

[0044] The preparation method of the above-mentioned composition includes the following steps: at 45°C, mixing Pseudostellariae Radix extract and hydrolyzed ginsenosides in proportion, adding 1,2-hexanediol, and stirring to dissolve completely; then adding Glycine max seed extract and stirring thoroughly, and finally adding deionized water and stirring to mix evenly.

[0045] Example 3

[0046] A composition comprising the following components in parts by weight:

[0047] 1 part of Pseudostellariae Radix Extract, 1 part of hydrolyzed ginsenosides, 3 parts of 1,2-hexanediol, 3 parts of Glycine Max Seed Extract, and the balance is deionized water.

[0048] The preparation method of the above-mentioned composition includes the following steps: at 45°C, mixing Pseudostellariae Radix extract and hydrolyzed ginsenosides in proportion, adding 1,2-hexanediol, and stirring to dissolve completely; then adding Glycine max seed extract and stirring thoroughly, and finally adding deionized water and stirring to mix evenly.

[0049] Example 4

[0050] A composition comprising the following components in parts by weight:

[0051] 10 parts of Pseudostellariae Radix extract, 10 parts of hydrolyzed ginsenosides, 3 parts of 1,2-hexanediol, 3 parts of Glycine max seed extract, and the balance is deionized water.

[0052] The preparation method of the above-mentioned composition includes the following steps: at 45°C, mixing Pseudostellariae Radix extract and hydrolyzed ginsenosides in proportion, adding 1,2-hexanediol, and stirring to dissolve completely; then adding Glycine max seed extract and stirring thoroughly, and finally adding deionized water and stirring to mix evenly.

[0053] Comparative Example 1 (the difference from Example 1 is that a single functional component is used)

[0054] A composition comprises the following components in parts by weight: 0.1 parts of Radix Pseudostellariae Extract, 3 parts of 1,2-hexanediol, 3 parts of Glycine Maxim Seed Extract, and the balance is deionized water. The preparation method is the same as that of Example 1.

[0055] Comparative Example 2 (the difference from Example 1 is that a single functional component is used)

[0056] A composition comprises the following components in parts by weight: 0.1 parts of hydrolyzed ginsenosides, 3 parts of 1,2-hexanediol, 3 parts of wild soybean seed extract, and the balance is deionized water. The preparation method is the same as that of Example 1.

[0057] Comparative Example 3 (the difference from Example 1 is that the Pseudostellariae Radix extract is replaced with palmitoyl pentapeptide-4 having similar efficacy)

[0058] A composition comprises the following components in parts by weight: 0.1 parts of palmitoyl pentapeptide-4, 0.1 parts of hydrolyzed ginsenosides, 3 parts of 1,2-hexanediol, 3 parts of wild soybean seed extract, and the balance is deionized water. The preparation method is the same as that of Example 1.

[0059] Comparative Example 4 (the difference from Example 1 is that the hydrolyzed ginsenosides are replaced with carnosine having similar efficacy)

[0060] A composition comprises the following components by weight: 0.1 part of Radix Pseudostellariae extract, 0.1 part of carnosine, 3 parts of 1,2-hexanediol, 3 parts of Glycine max seed extract, and the balance is deionized water. The preparation method is the same as that of Example 1.

[0061] Experimental Example 1: Composition Effect Test

[0062] Using human fibroblasts as a test system, the changes in the contents of major MMP-1, elastin, and type III collagen were used to evaluate whether the samples prepared in Examples 1-4 and Comparative Examples 1-4 had anti-wrinkle and firming effects.

[0063] The experimental design is shown in Table 1.

[0064] Table 1 Experimental design

[0065]

[0066] Experimental methods:

[0067] 1) Cell seeding: According to the appropriate seeding density (8×10 4 The cells were seeded into 24-well plates (100 μl / well) and incubated overnight in an incubator (37° C., 5% CO 2 ).

[0068] 2) Experimental grouping: The experiment set up a blank control group, a model group and a sample group; the sample group was the samples prepared in Examples 1-4 and Comparative Examples 1-4.

[0069] 3) Solution preparation: Prepare the test substance working solution at a mass detection concentration of 0.5%.

[0070] 4) Drug administration: When the cell plating rate in the 24-well plate reaches 40% to 60%, drug administration is performed; 1 mL of cell culture medium is added to each well of the blank control group and the model group; 1 mL of culture medium containing the test substance at the corresponding concentration is added to each well of the sample group; 3 parallel samples are set up for each group of experiments.

[0071] 5) Radiation: After the administration, the total dose received by the model group and the sample group was 9J / cm2 At the same time, the blank control group was placed in the same environment (UVA radiation dose of 0 J / cm 2 ).

[0072] 6) Detection: Add 0.5 mL of lysis buffer to each well and lyse by pipetting; extract RNA, reverse transcribe to cDNA, and perform fluorescence quantitative PCR detection using 2 -ΔΔCT Method to calculate the results.

[0073] Test results:

[0074] 1) MMP-1 gene expression test results:

[0075] According to the specific experimental method, MMP-1 gene expression detection was performed, and the detection results were as follows Figure 1 shown.

[0076] Depend on Figure 1 It can be seen that after UVA treatment, the expression of MMP-1 gene in the model group increased significantly by 20.13%, which shows that the model test is effective. Compared with the model group, Examples 1, 2, 3, and 4 all have good inhibition of MMP-1 gene expression, and are concentration-dependent. The higher the content of Pseudostellariae Radix Extract and hydrolyzed ginsenosides, the better the effect of inhibiting MMP-1 gene expression, and the effect is better than the samples prepared in Comparative Examples 1-4. It is proved that in the composition of the present invention, Pseudostellariae Radix Extract and hydrolyzed ginsenosides have good synergistic effects, and the components are irreplaceable.

[0077] 2) CollagenⅢ gene expression test results:

[0078] According to the specific experimental method, the expression of CollagenⅢ(COL3A1) gene was detected. The test results were as follows Figure 2 shown.

[0079] Depend on Figure 2 It can be seen that after UVA treatment, the gene expression of type III collagen in the model group was significantly reduced by 47.98%, indicating that the model group is effective. Compared with the model group, the samples of Examples 1-4 all have a better effect of promoting the expression of Collagen III, and are concentration-dependent, with significant differences, and the effects are significantly better than those of the samples of Comparative Examples 1-4. It is proved that in the composition of the present invention, the extract of Radix Pseudostellariae and the hydrolyzed ginsenosides have a good synergistic effect, and the components are irreplaceable.

[0080] 3) Elastin gene expression test results:

[0081] According to the specific experimental method, Elastin (ELN) gene expression detection was performed, and the test results were as follows Figure 3 shown.

[0082] Depend on Figure 3 It can be seen that after UVA treatment, the elastin gene expression in the model group was significantly reduced by 41.26%, indicating that the model group was effective. Compared with the model group, the samples of Examples 1-4 all had a better effect of promoting the expression of elastin, and were concentration-dependent, with significant differences, and the effects were significantly better than those of the samples of Comparative Examples 1-4. It is proved that in the composition of the present invention, the Pseudostellariae Radix extract and the hydrolyzed ginsenosides have a good synergistic effect, and the components are irreplaceable.

[0083] Application Example 1

[0084] The composition obtained in Example 1 of the present invention is used to prepare a skin cream, and the formula components of the skin cream are shown in Table 2.

[0085] Table 2 Skin cream formula

[0086]

[0087] The skin care cream is prepared according to a conventional preparation method.

[0088] Comparative application example 1

[0089] The only difference from Application Example 1 is that the composition of Example 1 is not contained, and other components, contents and preparation methods remain unchanged.

[0090] Experimental Example 2: Skin Elasticity Test

[0091] The skin elasticity tester MPA580 (CourageKhazaka, Germany) was used. The instrument sucked the skin into a specific probe under negative pressure, and the depth of the sucked skin was measured by a non-contact optical test system. The elastic properties of the skin were analyzed using MPA software. The ratio of the amount of skin rebound without negative pressure to the maximum amount of skin stretching with negative pressure was used to evaluate skin elasticity. The closer the ratio is to 1, the better the skin elasticity. In this experiment, the changes in the skin elasticity of the subjects were compared before and after using the skin creams of Application Example 1 and Comparative Application Example 1. A total of 30 subjects were included, including 20 women and 10 men, with an average age of 28 years old.

[0092] The specific method is: the subjects use the product on their face once in the morning and once in the evening every day, and conduct follow-up visits before use (D0), on the 14th day of use (D14) and D28 (28th day). During the follow-up visit, the skin needs to be thoroughly cleaned. Under the conditions of temperature 21±1℃ and humidity 50±10%, the subjects clean their faces and wait for 20 minutes before measuring the elasticity of the face (R2 value), and comprehensively evaluate the firming effect of the samples on human skin. The results are shown in Tables 3 and 4.

[0093] Elasticity (R2 value) change rate: refers to the change rate of skin elasticity after using the product compared to before use. A positive value means that the ratio of the maximum stretch displacement of the skin increases, and the skin elasticity is better; a negative value means that the ratio of the maximum stretch displacement of the skin decreases, and the skin elasticity is worse.

[0094] Table 3 Elasticity (R2) analysis results of application example 1

[0095] Apply sample time D0 D14 D28 R2 mean 0.6257 0.7800 0.9524 R2 change rate % —— 24.66％ 52.21％ sig(α=0.05) —— P＜0.05 P＜0.05 Analysis of significant difference —— There is a significant difference There is a significant difference

[0096] Table 4 Comparative Application Example 1 Elasticity (R2) Analysis Results

[0097] Apply sample time D0 D14 D28 R2 mean 0.5831 0.5917 0.5762 R2 change rate % —— 1.47％ -1.18％ Analysis of significant difference —— No significant difference No significant difference

[0098] It can be seen from the skin elasticity (R2) test data results in Tables 3 and 4 that after using the skin care cream of Application Example 1 (containing 2% of the composition of Example 1) for 2 weeks, the elasticity index of the skin of the subjects was significantly improved, and the effect was more obvious after 4 weeks of use, with the elasticity index R2 increased by 52.21%; while the skin elasticity index of the subjects using the product of Comparative Application Example 1 did not change significantly, further illustrating that the composition provided by the present invention has good effects on improving skin elasticity, improving skin firmness, and reducing wrinkles.

[0099] The preferred embodiments of the present invention are specifically described above, but the invention is not limited to the embodiments. Those skilled in the art may make various equivalent modifications or substitutions without violating the spirit of the invention. These equivalent modifications or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A composition, characterized in that The composition comprises the following components: Pseudostellaria heterophylla extract and hydrolyzed ginsenosides.

2. The composition according to claim 1, characterized in that The composition comprises the following components in parts by mass: 0.001-10 parts of Pseudostellariae Radix extract and 0.001-10 parts of hydrolyzed ginsenosides.

3. The composition according to claim 2, characterized in that The composition comprises the following components in parts by mass: 0.01-10 parts of Pseudostellariae Radix extract and 0.01-10 parts of hydrolyzed ginsenosides.

4. The composition according to claim 1, characterized in that The composition also includes a solvent and a solubilizer.

5. The composition according to claim 4, characterized in that The solvents include water and polyols.

6. The composition according to claim 4, characterized in that The solubilizing agent includes at least one of lecithin, soybean seed extract, hydroxypropyl cyclodextrin, PEG-40 hydrogenated castor oil and PEG-60 hydrogenated castor oil.

7. The method for preparing the composition according to any one of claims 1 to 6, characterized in that: The following steps are involved: The components are stirred and mixed to obtain the composition.

8. The preparation method according to claim 7, characterized in that: The following steps are involved: The Pseudostellariae Radix extract and the hydrolyzed ginsenosides are dissolved in polyol at 40-45° C., and then a solubilizer and water are added thereto, and the mixture is stirred and mixed to obtain the composition.

9. A cosmetic, characterized in that: The cosmetic comprises the composition according to any one of claims 1 to 6.

10. The cosmetic according to claim 9, characterized in that: The content of the composition is 0.01-20 wt%.

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