Application of honey in preparation of composition and method for improving skin affinity

Abstract

The invention relates to an application of honey in preparation of a composition and a method for improving skin affinity. The present invention provides the use of honey in the manufacture of a composition for improving skin affinity, the contact angle between water and the skin to which the composition is applied being 30 degrees or less. The present invention also provides a method for improving skin affinity, comprising applying the honey-containing composition to the skin, the contact angle between water and the skin to which the honey-containing composition is applied being 30 degrees or less.

Description

Technical Field

[0001] The present invention relates to a composition for improving skin affinity. Background Art

[0002] The stratum corneum of the skin acts as a barrier to the evaporation of water from the body and to the entry of viruses, substances, etc. from the outside, so it is difficult to transport a sufficient amount of compounds such as drugs or cosmetics from outside the skin to the inside of the skin. On the other hand, there are cosmetics on the market that are intended to promote the penetration of ingredients in the subsequently applied preparation into the skin (also called enhanced beauty essences or introduction essences (introduction beauty essences), etc.), and such cosmetics contain, for example, surfactants, polyols, and lactic acid.

[0003] For example, Japanese Patent Gazette No. 5022583 discloses a skin care pretreatment method as a method for improving the affinity and permeability of subsequent skin care cosmetics, which is characterized by comprising the following steps: step (1) washing the skin with a skin cleanser and then rinsing the cleanser with water; step (2) shaking a skin rinse composition and applying it in a dispersed state to the wet skin, wherein the composition is a composition having an aqueous phase and an oil phase that is liquid at room temperature, and the oil phase and the aqueous phase are separated into two layers, wherein the oil phase accounts for 3 to 30% by mass in the composition and contains 1% by mass or more of a higher fatty acid that is liquid at room temperature; and step (3) rinsing the skin rinse composition with water.

[0004] In addition, regarding compositions for application to the skin, for example, Japanese Patent Gazette No. 6888835 discloses a fermentation product obtained by fermenting honey and German chamomile or a processed product thereof with Lactobacillus kunsii, and states that the fermentation product can be used for improving skin barrier function, moisturizing the skin, improving skin elasticity, improving dull skin, improving skin wrinkles, improving rough skin, preventing skin aging, improving skin texture, and improving sensitive skin. In addition, O'Farrell C et al., "Formulation of an antibacterial topical cream containing bioengineered honey that generates reactive oxygen species." Biomater Adv. 2022Feb; 133: 112664. doi: 10.1016 / j.msec.2022.112664. Epub 2022Jan 20. recorded that a cream containing bioengineered medicinal honey (which has been shown to kill multidrug-resistant strains of bacteria by delivering reactive oxygen species) showed a low contact angle on pig skin while maintaining a bactericidal effect, and the cream spread well on the skin surface. Summary of the invention

[0005] Problems to be solved by the invention

[0006] An object of the present invention is to provide a novel composition for improving skin affinity.

[0007] Means for solving problems

[0008] The inventors of the present application have conducted intensive studies to achieve the above-mentioned object and, as a result, have found that when honey is applied to an artificial skin model, the contact angle between water and the artificial skin model to which honey is applied becomes 30 degrees or less, thereby completing the present invention.

[0009] That is, the present invention relates to, for example, the following aspects. [1]

[0011] A composition containing honey for improving skin affinity, wherein the contact angle between water and the skin to which the composition is applied is 30 degrees or less. [2]

[0013] The composition as described in [1] further comprises royal jelly, butylene glycol and pentylene glycol. [3]

[0015] The composition according to [1] or [2], further comprising a lactobacillus fermented extract of honey. [4]

[0017] The composition according to [3], wherein the lactobacillus fermented extract of honey is obtained by fermenting honey and German chamomile flowers using Lactobacillus kunsii. [5]

[0019] The composition according to any one of [1] to [4], wherein the honey is manuka honey and / or acacia honey. [6]

[0021] Use of honey in the manufacture of a composition for improving skin affinity, wherein the contact angle between water and the skin to which the composition is applied is 30 degrees or less. [7]

[0023] The use as described in [6], wherein the composition further contains royal jelly, butylene glycol and pentylene glycol. [8]

[0025] The use as described in [6] or [7], wherein the composition further contains a lactobacillus fermentation extract of honey. [9]

[0027] The use as described in [8], wherein the lactobacillus fermented extract of honey is obtained by fermenting honey and German chamomile flowers using Lactobacillus kunsii.

[10]

[0029] The use according to any one of [6] to [9], wherein the honey is manuka honey and / or acacia honey.

[11]

[0031] The use as described in any one of [6] to

[10] , wherein the composition contains 5% to 60% by mass of honey, 0.001% to 0.5% by mass of royal jelly, 0.1% to 10% by mass of butylene glycol, 0.01% to 2.5% by mass of pentylene glycol, and 25% to 35% by mass of a lactobacillus fermentation extract of honey, relative to the total amount of the composition.

[12]

[0033] A method for improving skin affinity, comprising applying a composition containing honey to the skin, wherein the contact angle between water and the skin to which the composition is applied is 30 degrees or less.

[13]

[0035] The method as described in

[12] , wherein the honey-containing composition further contains royal jelly, butylene glycol and pentylene glycol.

[14]

[0037] The method according to

[12] or

[13] , wherein the honey-containing composition further contains a lactobacillus fermentation extract of honey.

[15]

[0039] The method according to any one of

[12] to

[14] , wherein the lactobacillus fermentation extract of honey is obtained by fermenting honey and German chamomile flowers using Lactobacillus kunsii.

[16]

[0041] The method according to any one of

[12] to

[15] , wherein the honey is manuka honey and / or acacia honey.

[17]

[0043] A method as described in any one of

[12] to

[16] , wherein the composition containing honey contains 5% to 60% by mass of honey, 0.001% to 0.5% by mass of royal jelly, 0.1% to 10% by mass of butylene glycol, 0.01% to 2.5% by mass of pentylene glycol, and 25% to 35% by mass of a lactobacillus fermentation extract of honey, relative to the total amount of the composition.

[0044] Effects of the Invention

[0045] According to the present invention, a novel composition for improving skin affinity can be provided. DETAILED DESCRIPTION

[0046] Hereinafter, the mode for implementing the present invention will be described in detail. However, the present invention is not limited to the following embodiment.

[0047] 〔Composition〕

[0048] The composition for improving skin affinity according to the present embodiment (hereinafter also referred to as “composition”) contains honey, and the contact angle between water and the skin to which the composition is applied is 30 degrees or less.

[0049] As for the composition involved in the present embodiment, the contact angle between water and the skin to which the composition is applied is less than 30 degrees, which has the effect of improving skin affinity. Here, in this specification, the so-called "improving skin affinity" refers to improving in a way that a hydrophobic or hydrophilic liquid is closer to the skin, which can be evaluated in the following way: whether the contact angle between water and the skin when water is dropped onto the skin (skin) to which the above-mentioned composition is applied is smaller than the contact angle of the skin to which the above-mentioned composition is not applied. If the contact angle of the above-mentioned water becomes smaller after the above-mentioned composition is applied, it can be judged that the skin affinity is improved. In addition, usually, the contact angle of water is used as an indicator for wettability evaluation. Therefore, the composition involved in the present embodiment can also be referred to as a composition for improving the wettability of the skin.

[0050] In the determination of the contact angle of water, the skin to which the composition involved in the present embodiment is applied can be natural skin or artificial skin. In addition, natural skin can be the skin of animals such as humans, preferably human skin, and in the case of human skin, it can be the skin of cheeks, forearms, etc., preferably skin without hair (hair removed). In the case of artificial skin, for example, it can also be skin that imitates human skin, it can also be artificial skin obtained by differentiation induction using iPS cells, it can also be an artificial skin model using polyurethane as raw material, etc. When it is judged that the contact angle of water and the skin to which the composition is applied is less than 30 degrees, the skin is the skin of the forearm or the artificial skin model using polyurethane as raw material, 0.1g of the composition is uniformly applied to the skin in the region of 5cm×5cm, 2μL of water is added after 1 hour, and in addition, the contact angle is determined by the contact angle of water under such conditions just after adding water (pure water such as distilled water) (after 1000 milliseconds).

[0051] The contact angle of water can be measured using, for example, an automatic contact angle meter DMo-602 (manufactured by Kyowa Interface Science Co., Ltd.). The contact angle of water can be measured, for example, by a drop method, etc. The contact angle of water can be analyzed (calculated) by, for example, the θ / 2 method, the tangent method, etc.

[0052] The contact angle of water on human skin varies according to the part. In addition, the contact angle of water on the forearm of a person is usually about 90 degrees, and artificial skin also has a contact angle of water close to that on the forearm of a person. The skin of animals other than humans preferably uses a skin having a contact angle of water of about 90 degrees. With regard to the composition involved in the present embodiment, the contact angle of water with the skin when water is dripped onto the skin (skin) to which the composition is applied is preferably reduced by more than 50 degrees, more than 55 degrees, more than 60 degrees, or more than 65 degrees compared to the contact angle of water with the skin to which the above-mentioned composition is not applied.

[0053] With regard to the composition involved in this embodiment, the contact angle between water and the skin to which the composition is applied may be 30 degrees or less, for example, it may be 28 degrees or less, 26 degrees or less, 24 degrees or less, 22 degrees or less, 20 degrees or less, 18 degrees or less, 16 degrees or less, 14 degrees or less, or 13 degrees or less, and may be 0 degrees or more, 1 degree or more, 3 degrees or more, or 5 degrees or more.

[0054] The composition according to the present embodiment is applied to the skin, and can be applied to the skin of any part as long as it is skin. In the present specification, "application" may be coating or spreading.

[0055] Honey is made from honey collected by bees from nectar of plants, sap of trees, secretions of insects that parasitize plants, etc. as the main ingredient.

[0056] The type of honey contained in the composition involved in the present embodiment is not particularly limited, and for example, nectar honey, Ulmo honey, Manuka honey, acacia honey, linden honey, Rewa Rewa honey, multiflora honey, clover honey, orange honey, clover honey, rosemary honey, sunflower honey, rape honey, coffee honey, coriander (Coriandrum) honey, Tochigi honey, buckwheat honey, Japanese beech honey, holly honey, holly honey, dogwood honey, etc. The honey is preferably Manuka honey and / or acacia honey.

[0057] Manuka honey is honey that can be collected from the flowers of manuka (Leptospermum scoparium), which is a member of the genus Tamarix in the family Myrtaceae. Acacia honey is honey that can be collected from the flowers of Robinia pseudoacacia, which is a member of the genus Robinia in the family Fabaceae.

[0058] The type of bees used to collect honey is not particularly limited. Honey can be obtained as a beekeeping product, for example, according to a conventional method.

[0059] Honey is generally kept at about 35° C. by the body temperature of bees while stored in a beehive. Honey collected from a beehive is generally heated as necessary to reduce viscosity for ease of transportation and filling and to melt crystals.

[0060] The collected honey may remain in the collected state, or may be subjected to treatments such as removal of proteins, wax, garbage, bee and nest residues, filtration, extraction with organic solvents, purification of components, sterilization, concentration, etc. before or after heating.

[0061] Honey is known to have a moisturizing effect, but it is not known to have an effect of improving skin affinity.

[0062] The content of honey in the composition involved in the present embodiment may be, for example, 5% by mass or more, 10% by mass or more, 15% by mass or more, 20% by mass or more, 25% by mass or more, or 30% by mass or less, or 100% by mass or less, 90% by mass or less, 80% by mass or less, 70% by mass or less, or 60% by mass or less, relative to the total amount of the composition.

[0063] The composition according to the present embodiment may further contain royal jelly, butylene glycol, and pentylene glycol. As a result, the contact angle between water and the skin to which the composition according to the present embodiment is applied becomes smaller, and the skin affinity is further improved.

[0064] Royal jelly in the present invention includes raw royal jelly, dried royal jelly, dried royal jelly powder, enzyme-treated royal jelly, royal jelly extract, royal jelly fermentation product, etc. In addition, the production area of ​​royal jelly can be any region in European countries, Oceania countries, the United States, Brazil, Japan, China, other Asian countries, etc.

[0065] The dried royal jelly powder is obtained by drying and powdering raw royal jelly. As the drying method, any known method used in general food processing such as natural drying such as ventilation drying and sun drying, forced drying by heating with electricity, etc., and freeze drying can be used. Freeze drying is preferred.

[0066] Enzyme-treated royal jelly is obtained by treating royal jelly with a protease (protease). Preferably, it is low-allergenic enzyme-treated royal jelly in which allergic reactions caused by proteins contained in royal jelly are suppressed by protease treatment. Therefore, in addition to the protease decomposition products of proteins contained in royal jelly, the enzyme-treated royal jelly may also contain the aforementioned saturated fatty acids, organic acids such as unsaturated fatty acids, lipids, sugars, vitamins, and various minerals.

[0067] The royal jelly used for the production of the enzyme-treated royal jelly is not particularly limited, and examples thereof include raw royal jelly, royal jelly powder obtained by drying and powdering raw royal jelly, and royal jelly extracted from raw royal jelly with water or hydrous ethanol.

[0068] The production of enzyme-treated royal jelly can be carried out by treating the royal jelly raw material with an enzyme having at least an endopeptidase action, an enzyme having at least an exopeptidase action, and / or an enzyme having both an endopeptidase action and an exopeptidase action.

[0069] Examples of protease having at least endopeptidase activity include endopeptidases derived from animals (e.g., trypsin, chymotrypsin, etc.), endopeptidases derived from plants (e.g., papain, etc.), and endopeptidases derived from microorganisms (e.g., lactic acid bacteria, yeast, mold, Bacillus subtilis, actinomycetes, etc.).

[0070] Examples of the proteolytic enzyme having at least exopeptidase activity include carboxypeptidase, aminopeptidase, exopeptidase derived from microorganisms (e.g., lactic acid bacteria, Aspergillus, Rhizopus, etc.), and pancreatin and pepsin which also have endopeptidase activity.

[0071] Preferred examples of enzymes having both exopeptidase activity and endopeptidase activity among such various enzymes include peptidase produced by Streptomyces griseus (trade name: ACTINASE AS), peptidase produced by Aspergillus oryzae (trade name: Protease A, Flavourzyme, Protease), and peptidase produced by Aspergillus melleus (trade name: Protease P). Preferred examples of enzymes having exopeptidase activity include peptidase produced by Aspergillus oryzae (trade name: Umamizyme G, Promod 192P, Promod 194P, Sumizyme FLAP), peptidase produced by Aspergillus sojae (trade name: Sternzyme B15024), and peptidase produced by Aspergillus genus (trade name: Kokulase P), peptidase produced by Rhizopus oryzae (trade name: Peptidase R). In addition, preferred examples of enzymes having endopeptidase activity include peptidase produced by Bacillus subtilis (trade name: Orientase22BF, Nukureishin), peptidase produced by Bacillus licheniformis (trade name: Alcalase), peptidase produced by Bacillus stearothermophilus (trade name: Protease S), peptidase produced by Bacillus amyloliquefaciens (trade name: Neutrase), and peptidase produced by Bacillus (trade name: Protamex).

[0072] The enzyme treatment for reducing the allergenicity of royal jelly can be performed according to the description of, for example, JP-A-2007-295919 and JP-A-2007-295920.

[0073] Royal jelly extract is obtained by extracting royal jelly (including raw, dried and ground products) using water or aqueous ethanol.

[0074] Royal jelly fermentation products can be produced by conventional methods using microorganisms such as yeast and lactic acid bacteria.

[0075] The content of royal jelly in the composition involved in this embodiment can be, for example, 0.001 mass% or more, 0.005 mass% or more, 0.01 mass% or more, 0.05 mass% or more, 0.1 mass% or more, or 0.2 mass% or more, or it can be less than 10 mass%, less than 5 mass%, less than 1 mass%, or less than 0.5 mass%, relative to the total amount of the composition.

[0076] Butanediol is a compound also known as butanediol, and is a substance mostly used as a humectant. In this specification, butanediol refers to 1,3-butanediol. The content of butanediol in the composition involved in the present embodiment can be, for example, 0.1% by mass or more, 0.5% by mass or more, 1% by mass or more, 2% by mass or more, 3% by mass or more, 4% by mass or more, 5% by mass or more, 6% by mass or more, 7% by mass or more, or 8% by mass or more, or 30% by mass or less, 25% by mass or less, 20% by mass or less, 15% by mass or less, or 10% by mass or less.

[0077] Pentylene glycol is a compound also known as 1,2-pentanediol, and is a substance that is often used as a moisturizer. The content of pentylene glycol in the composition of the present embodiment may be, for example, 0.01% by mass or more, 0.05% by mass or more, 0.1% by mass or more, 0.5% by mass or more, 1% by mass or more, or 2% by mass or less, or 10% by mass or less, 8% by mass or less, 6% by mass or less, 4% by mass or less, or 2.5% by mass or less.

[0078] The composition according to the present embodiment may further contain a lactobacillus fermented extract of honey. As a result, the contact angle between water and the skin to which the composition according to the present embodiment is applied is further reduced, and the skin affinity is further improved.

[0079] The lactobacillus fermented extract of honey is obtained by fermenting honey using lactobacillus, and is preferably obtained by fermenting honey and German chamomile flowers using lactobacillus.

[0080] German chamomile is a plant of the Asteraceae family (Compositae or Asteraceae), and its scientific name is Matricariachamomilla L. or Matricaria recutita L.

[0081] The German chamomile flowers used for fermentation may be flowers obtained by collecting German chamomile flowers, or may be processed materials of German chamomile flowers. As processed materials of German chamomile flowers, for example, processed materials obtained by drying, pulverizing, extracting, etc. of German chamomile flowers can be cited. These treatments may be performed alone or in combination of two or more.

[0082] The processed material of German chamomile flowers can be, for example, dried materials, crushed materials, crushed materials, powders, extracts, etc. of German chamomile flowers. The drying of German chamomile flowers can be performed, for example, by sun drying, hot air drying, freeze drying, spray drying, etc. The crushing and crushing can be performed using, for example, a grinder, a crusher, etc., respectively. The flowers of German chamomile are preferably dry powders of German chamomile flowers. The dry powders of German chamomile flowers can be obtained, for example, by drying the flowers of German chamomile and then crushing them. The flowers of German chamomile are preferably obtained by drying and crushing them immediately after collection. As the processed material of German chamomile flowers, commercial products can be used.

[0083] The extract of German chamomile flowers can be obtained by extracting German chamomile flowers using a method commonly used in plant extraction. For example, German chamomile flowers as an extraction raw material can be put into a treatment tank filled with an extraction solvent, and the soluble components can be dissolved while appropriately stirring as needed, and then filtered to remove the extraction residue, thereby obtaining an extract.

[0084] As the extract of German chamomile flowers, an extract obtained by extracting German chamomile flowers may be used as it is, or an extract obtained by further dilution, concentration, drying, filtration, purification, etc. may be used.

[0085] As the extraction solvent, for example, it can be an aqueous solvent, a hydrophilic organic solvent, etc. As the aqueous solvent, for example, pure water, purified water, tap water, well water, mineral water, mineralized water, hot spring water, spring water, fresh water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, etc. can be mentioned. As the hydrophilic organic solvent, for example, it can be: methanol, ethanol, propanol, isopropanol and other lower alcohols with 1 to 5 carbon atoms; acetone, methyl ethyl ketone and other lower aliphatic ketones; 1,3-butanediol, propylene glycol, glycerol and other polyols with 2 to 5 carbon atoms, etc. The extraction solvent can be used alone or in combination of two or more.

[0086] The fermentation of honey or the fermentation of honey and German chamomile flowers is performed using lactobacilli. Examples of lactobacilli include bacteria belonging to the genus Lactobacillus, and more specifically, Lactobacillus kunsii is preferred.

[0087] Lactobacillus kunsii preferably shows assimilation to glucose and fructose, and more preferably shows assimilation to glucose, fructose, sucrose, trehalose and gluconate. Lactobacillus kunsii may be Lactobacillus kunsii BPS402. Lactobacillus kunsii BPS402 was deposited at the Patent Organisms Depository of the National Institute of Advanced Industrial Science and Technology (1-1-1, Central 6, Higashi, Tsukuba, Ibaraki, Japan, zip code 305-8566) on October 3, 2011 under the deposit number FERM P-22177 and can be obtained. In addition, the strain is currently transferred to an international deposit with the deposit number FERM BP-11439.

[0088] The culture of lactobacillus used in the fermentation can be carried out according to the usual method. The culture medium is not particularly limited as long as it can culture the bacteria, and natural culture medium, synthetic culture medium, semi-synthetic culture medium, etc. can be used. As a culture medium, a culture medium containing a nitrogen source and a carbon source can be used. As a nitrogen source, meat extract, peptone, casein, yeast extract, gluten, soy flour, soy hydrolyzate, amino acids, etc. can be mentioned, and as a carbon source, glucose, lactose, fructose, inositol, sorbitol, water, starch, koji juice, wheat bran, bagasse, molasses, etc. can be mentioned. In addition, inorganic matter (for example, ammonium sulfate, potassium phosphate, magnesium chloride, salt, calcium carbonate, iron, manganese, molybdenum), various vitamins, etc. can be added.

[0089] The culture temperature can be, for example, 4 to 45° C., preferably 25 to 40° C., and more preferably 28 to 33° C. The culture time can be, for example, 8 to 72 hours. The culture can be carried out by aeration shaking or aeration stirring. The pH of the culture medium can be, for example, 4.0 to 9.0, preferably 6.0 to 8.0. As a culture method, for example, the bacteria can be inoculated into an MRS medium and cultured at 30° C. for 48 hours.

[0090] The bacterial solution obtained by culturing lactobacilli may be directly inoculated into a fermentation medium containing honey or a fermentation medium containing honey and German chamomile flowers for fermentation, or may be diluted with a culture medium or the like for use.

[0091] The fermentation of honey by lactobacillus or the fermentation of honey and German chamomile flowers can be carried out at 4 to 45° C., preferably at 25 to 40° C. The fermentation time can be, for example, 12 to 60 hours, preferably 24 to 48 hours. The pH of the honey-containing substance at the start of fermentation can be, for example, 3 to 8, preferably 4 to 6. The fermentation can be carried out with aeration shaking or aeration stirring.

[0092] The fermentation of honey or the fermentation of honey and German chamomile flowers can be carried out by culturing lactobacilli in a fermentation medium containing honey or a fermentation medium containing honey and German chamomile flowers. The fermentation medium containing honey or the fermentation medium containing honey and German chamomile flowers can be in any form that lactobacilli can ferment, for example, in the form of a liquid, a paste, a gel, etc. The fermentation medium can be, for example, obtained by dissolving or suspending honey or honey and German chamomile flowers in a solvent such as water.

[0093] The concentration of honey in the fermentation medium at the start of fermentation can be, for example, 0.1 to 40%, 1 to 30%, or 8 to 13% relative to the total amount of the fermentation medium. In addition, the concentration of honey in the fermentation medium at the start of fermentation can be, for example, 0.08 to 32%, 0.8 to 24%, or 6.4 to 10.4% relative to the total amount of the fermentation medium in terms of solid content.

[0094] The concentration of German chamomile flowers in the fermentation medium at the start of fermentation can be, for example, 0.01 to 20%, 0.1 to 5%, or 0.5 to 2% in terms of solid content relative to the total amount of the fermentation medium. The mass ratio of honey (total amount) to German chamomile flowers (dry mass) in the fermentation medium at the start of fermentation can be, for example, 4000:1 to 1:200, or 26:1 to 4:1. The fermentation medium can also contain known nutrients required for the cultivation of lactic acid bacteria, such as vitamins, amino acids, minerals, salts, surfactants, fatty acids, and metals. The honey used in the fermentation can be sterilized by heating or the like.

[0095] The lactobacillus fermented extract of honey or the lactobacillus fermented extract of honey and German chamomile flowers may be a substance obtained by fermentation itself, or may be a substance obtained by heating, filtering, purifying, concentrating, evaporating to dryness, freeze drying, spray drying, etc. The lactobacillus fermented extract of honey or the lactobacillus fermented extract of honey and German chamomile flowers may contain live and / or dead cells of lactobacillus, and the cells may be separated and removed by filtration or the like.

[0096] The content of the lactobacillus fermentation extract of honey in the composition involved in the present embodiment can be, for example, 25 mass % or more, 26 mass % or more, 27 mass % or more, 28 mass % or more, or 29 mass % or less, and can be 35 mass % or less, 34 mass % or less, 33 mass % or less, 32 mass % or less, 31 mass % or less, or 30 mass % or less.

[0097] The composition according to the present embodiment may be, for example, a cosmetic or a cosmetic raw material.

[0098] The cosmetic raw material may be in any form such as solid, liquid, paste, etc. The cosmetic may be a medicinal cosmetic (ie, a quasi-drug). The cosmetic includes all cosmetics that can be applied to the skin of animals (especially humans).

[0099] The cosmetics may contain ingredients permitted as cosmetics in addition to honey, royal jelly, butylene glycol, pentylene glycol, lactobacillus fermented extract of honey or lactobacillus fermented extract of honey and German chamomile flowers. Examples of ingredients permitted as cosmetics include whitening agents, moisturizers, antioxidants, oily ingredients, ultraviolet absorbers, surfactants, thickeners, alcohols, powder ingredients, colorants, aqueous ingredients, water, and various skin nutrients.

[0100] The dosage form of cosmetics may be, for example, soluble, emulsified, powdered, oily, gel, ointment, aerosol, water-oil two-layer, water-oil-powder three-layer, etc. As cosmetics, for example, facial cleansers, lotions, emulsions, creams, gels, essences, beauty serums, smearable masks, sheet masks, liquid sprays (mist), basic cosmetics such as UV protection cosmetics, makeup cosmetics such as foundation, lipstick, blush, eye shadow, eyeliner (pen), mascara, massage agents, cleansers, aftershaves, preshaves, shaving creams, bath agents, soaps, hand creams, hand sanitizers, etc.

[0101] The composition of this embodiment can be used particularly suitably as an enhanced beauty serum (introduction liquid (introduction beauty serum)) or its raw material. An enhanced beauty serum refers to a substance that improves the skin affinity of cosmetics such as lotions and emulsions applied after the beauty serum is applied. The composition of this embodiment is a substance that improves skin affinity, and therefore can be suitably used in cosmetics for such purposes.

[0102] The dosage of the composition of the present embodiment when administered parenterally may be once a day, twice a day, three times a day, or multiple times. The dosage may vary depending on the health status of the subject, the combination with other compositions, the site to be applied, the application range, etc., and thus can be set arbitrarily by a person skilled in the art as needed. For example, the dosage when applied once to the skin may be 0.001 to 10 mg / cm 2 , preferably 0.001 to 8 mg / cm 2 , more preferably 0.001 to 6 mg / cm 2 .

[0103] The method for producing the composition involved in the present embodiment is not particularly limited, and a known method can be appropriately used. For example, a composition for the above purpose can be obtained by mixing honey, or royal jelly, butylene glycol, pentylene glycol, a lactobacillus fermented extract of honey, etc., which are added as needed, into an intermediate product or a final product in the manufacturing process of cosmetics.

[0104] [Methods to improve skin affinity]

[0105] One embodiment of the method for improving skin affinity includes applying the above-mentioned composition containing honey to the skin. As described above, the contact angle between water and the skin to which the composition containing honey is applied is less than 30 degrees. By applying the above-mentioned honey, the skin affinity is improved compared to the case where honey is not applied to the skin. The above-mentioned method for improving skin affinity may include also applying the above-mentioned royal jelly, the above-mentioned butylene glycol and the above-mentioned pentylene glycol to the skin. In this case, these ingredients can also be included in the above-mentioned composition containing honey and applied to the skin. In addition, the above-mentioned method for improving skin affinity may include also applying the lactobacillus fermented extract of the above-mentioned honey. In this case, the lactobacillus fermented extract of the above-mentioned honey can also be included in the above-mentioned composition containing honey and applied to the skin. The lactobacillus fermented extract of the above-mentioned honey can be obtained by fermenting honey and German chamomile flowers using Lactobacillus kunsii. In addition, the above-mentioned honey can be Manuka honey and / or acacia honey.

[0106] In one embodiment of the method for improving skin affinity, the above-mentioned honey-containing composition contains, relative to the total amount of the composition, 5% to 60% of honey, 0.001% to 0.5% of royal jelly, 0.1% to 10% of butylene glycol, 0.01% to 2.5% of pentylene glycol, and 25% to 35% of a lactobacillus fermentation extract of honey.

[0107] 〔use〕

[0108] The use according to one embodiment is use of honey in the production of a composition for improving skin affinity, wherein the contact angle between water and the skin to which the composition is applied is 30 degrees or less.

[0109] The composition, i.e., the composition containing honey, may be a composition further containing royal jelly, butylene glycol and pentylene glycol. In this case, the composition containing honey may further contain the lactobacillus fermented extract of honey, and the lactobacillus fermented extract of honey may be obtained by fermenting honey and German chamomile flowers using Lactobacillus kunsii. In addition, the honey may be Manuka honey and / or acacia honey.

[0110] In the use involved in one embodiment, the above-mentioned honey-containing composition can contain 5% to 60% by mass of honey, 0.001% to 0.5% by mass of royal jelly, 0.1% to 10% by mass of butylene glycol, 0.01% to 2.5% by mass of pentylene glycol, and 25% to 35% by mass of a lactobacillus fermentation extract of honey, based on the total amount of the composition.

[0111] Example

[0112] Hereinafter, the present invention will be described in more detail based on examples. However, the present invention is not limited to the following examples.

[0113] [Test example: Evaluation of skin affinity improvement effect by measuring water contact angle]

[0114] The contact angle between water and the skin to which each sample shown in Table 1 was applied was measured according to the following procedure to evaluate the effect of improving skin affinity.

[0115] 0.1 g of samples 1 to 5 shown in Table 1 were applied to a 5 cm × 5 cm area on a biological skin plate (manufactured by Beaulax), and then each sample was dried for more than 1 hour. Next, 2 μl of water was dripped onto the area where each sample was applied, and the contact angle of water after 1000 milliseconds was measured by the drop method. The contact angle of water was measured using an automatic contact angle meter DMo-602 (manufactured by Kyowa Interface Science Co., Ltd.) and analyzed by the θ / 2 method. The results are shown in Table 2.

[0116] [Table 1]

[0117]

[0118] [Table 2]

[0119] Uncoated Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Contact angle (°) 92 29.7 28.5 14.5 23.3 12.6

[0120] In the case of no application, the contact angle between water and the skin was 92 degrees. On the other hand, the contact angle between water and the skin after applying samples 1 and 2 was less than 30 degrees. This shows that by applying honey to the skin, the skin affinity is improved. In addition, compared with the contact angle between water and the skin after applying samples 1 and 2, the contact angle between water and the skin after applying sample 5 is smaller. This shows that by applying royal jelly, butylene glycol, pentylene glycol, and honey and lactobacillus fermented extract of German chamomile flowers to the skin in addition to honey, the skin affinity is further improved.

Claims

1. Use of honey in the manufacture of a composition for improving skin affinity, wherein the contact angle between water and skin to which the composition is applied is 30 degrees or less.

2. The use according to claim 1, wherein The composition also contains royal jelly, butylene glycol and pentylene glycol.

3. The use according to claim 2, wherein The composition also contains a lactobacillus fermented extract of honey.

4. The use according to claim 3, wherein The lactobacillus fermented extract of honey is obtained by fermenting honey and German chamomile flowers using Lactobacillus kunsii.

5. The use according to claim 1, wherein The honey is Manuka honey and / or acacia honey.

6. The use according to any one of claims 1 to 5, wherein The composition contains, relative to the total amount of the composition, 5% to 60% honey, 0.001% to 0.5% royal jelly, 0.1% to 10% butylene glycol, 0.01% to 2.5% pentylene glycol, and 25% to 35% lactobacillus fermentation extract of honey.

7. A method for improving skin affinity, comprising applying a composition containing honey to the skin, wherein the contact angle between water and the skin to which the composition containing honey is applied is 30 degrees or less.

8. The method of claim 6, wherein: The honey-containing composition further comprises royal jelly, butylene glycol and pentylene glycol.

9. The method of claim 7, wherein: The honey-containing composition further comprises a lactobacillus fermented extract of honey.

10. The method of claim 8, wherein: The lactobacillus fermented extract of honey is obtained by fermenting honey and German chamomile flowers using Lactobacillus kunsii.

11. The method of claim 7, wherein: The honey is Manuka honey and / or acacia honey.

12. The method according to any one of claims 7 to 11, wherein: The honey-containing composition contains, relative to the total amount of the composition, 5% to 60% honey, 0.001% to 0.5% royal jelly, 0.1% to 10% butylene glycol, 0.01% to 2.5% pentylene glycol, and 25% to 35% lactobacillus fermentation extract of honey.

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