Liquid heating and supplying device, liquid supplying method and cell culture equipment

Abstract

The invention relates to the technical field of life science instruments, and discloses a liquid heating and supplying device, a liquid supplying method and cell culture equipment. The liquid heating and supplying device comprises two heating bodies which can be arranged in a buckled mode, a consumable bag and a heating assembly, an outlet pipe and an inlet pipe of the consumable bag are both communicated with the bag body, the end, away from the bag body, of the inlet pipe is communicated with a liquid pipe and an air pipe, and the inlet pipe can be selectively communicated with the liquid pipe and the air pipe; the heating assembly comprises heating pieces and heat conduction blocks which are arranged in a one-to-one correspondence mode, the heat conduction blocks are arranged on the sides, close to each other, of the heating bodies, the heating pieces are arranged between the heat conduction blocks and the heating bodies, placing grooves are formed in the sides, away from the heating pieces, of the heat conduction blocks, the two placing grooves are buckled to form a placing space, and the bag body can be placed in the placing space. According to the invention, liquid required in cell culture setting can be preheated, the success rate of cell culture is improved, and meanwhile, the liquid in the consumable bag can be fully discharged, so that waste is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of life science instruments, and in particular to a liquid heating and supplying device, a liquid supplying method and a cell culture device. Background Art

[0002] Usually, the cell culture device generally includes the functions of cell fluid replacement and cell passage. During the cell fluid replacement process, new culture medium needs to be added to the cell culture bottle; during the cell passage process, digestive enzymes, culture medium and other culture solutions need to be added to the cells. Usually, the incubation temperature of cells in the cell culture incubator is 37°C, and the culture conditions of some stem cells are higher. Their growth state is more obviously affected by external temperature, carbon dioxide concentration, vibration and other conditions. Therefore, in order to provide a better environment for cell growth, the temperature of the added liquid needs to be adjusted to match the cell incubation temperature in the steps involving fluid addition such as fluid replacement.

[0003] In the prior art, when adding new culture medium or other solutions to cell culture bottles, culture dishes, etc., the added liquid is usually at room temperature, or directly filled with low-temperature liquid taken out of the refrigerator. Adding low-temperature liquid will seriously affect or even kill cells with high growth requirements, resulting in culture failure. In addition, although some equipment will place a large-capacity liquid heating transfer station in the working area to preheat the liquid, the preheated liquid cannot usually be recycled to the refrigerated storage area, so the heated liquid cannot be stored for a long time. After the liquid replacement and subculture work of the cell culture bottle is completed for a day, the liquid remaining in the transfer station needs to be emptied into the waste liquid area. This will cause a waste of culture reagents.

[0004] Therefore, there is an urgent need for a liquid heating and supplying device, a liquid supplying method and a cell culture equipment to solve the above problems. Summary of the invention

[0005] Based on the above, the purpose of the present invention is to provide a liquid heating and supply device, a liquid supply method and a cell culture device, which can preheat the liquid required in the cell culture device to improve the success rate of cell culture, and at the same time, the liquid in the consumable bag can be fully drained to avoid waste.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] Liquid heating and supply device, comprising:

[0008] Two heating bodies that can be snapped together;

[0009] A consumable bag, comprising a bag body, an outlet pipe and an inlet pipe, wherein the outlet pipe and the inlet pipe are both connected to the bag body, an end of the inlet pipe away from the bag body is respectively connected to a liquid pipe and an air pipe, and the inlet pipe can selectively connect to the liquid pipe and the air pipe;

[0010] The heating assembly includes a heating element and a heat-conducting block arranged in a one-to-one correspondence. The heat-conducting block is arranged on the side of the heating body close to each other, the heating element is arranged between the heat-conducting block and the heating body, and a placement groove is arranged on the side of the heat-conducting block away from the heating element. The two placement grooves are buckled to form a placement space, and the bag body can be placed in the placement space.

[0011] As a preferred solution of the liquid heating and supplying device, one end of the two heating bodies is rotatably connected, and the other end is provided with a locking assembly for locking the two heating bodies.

[0012] As a preferred solution of the liquid heating and supplying device, a heat-insulating layer is provided on a side of the heating element away from the heat-conducting block.

[0013] As a preferred solution of the liquid heating and supplying device, the heat conducting block is provided with a plurality of the placement grooves at intervals along its length direction.

[0014] As a preferred solution of the liquid heating and supplying device, the air pipe is provided with a filter.

[0015] As a preferred solution of the liquid heating and supplying device, the liquid heating and supplying device is further provided with a temperature detecting component, and the temperature detecting component is used to detect the temperature of the placement space.

[0016] As a preferred solution of the liquid heating and supplying device, the bag body is made of PP film, and the PP film is provided with an aluminum layer.

[0017] As a preferred solution of the liquid heating and supply device, the consumable bag also includes a three-way valve, which is respectively connected to the inlet pipe, the liquid pipe and the air pipe; or the consumable bag also includes two switch valves, which are respectively arranged on the liquid pipe and the air pipe.

[0018] A liquid supply method, using the liquid heating and supply device described in any of the above schemes; the volume of liquid in the inlet pipe is a, the volume of liquid in the bag body is b, the volume of liquid in the outlet pipe is c, and the required liquid volume is d. The liquid supply method includes: when d≤a+b+c, directly discharging the liquid with a volume of d in the consumable bag through the outlet pipe; when d>a+b+c, first injecting a volume of 1.5 (dabc) of liquid into the consumable bag through the inlet pipe and heating it, and then discharging the liquid with a volume of d in the consumable bag through the outlet pipe.

[0019] A cell culture device comprises the liquid heating and supply device described in any of the above schemes.

[0020] The beneficial effects of the present invention are:

[0021] The present invention provides a consumable bag for holding the liquid to be preheated. A heating body is provided to heat the liquid to be preheated in the bag body; specifically, the heating body is provided with a heating element and a heat-conducting block, wherein the heating element is used to provide high temperature, and the heat-conducting block is provided between the bag body and the heating element, for uniform temperature, and reducing the problem of failure of the liquid to be preheated due to excessive local temperature; and the heat-conducting block is provided with a placement groove, when the two heating bodies are buckled, the two placement grooves and the placement space that can completely cover the bag body are formed, and the embracing heating can effectively ensure sufficient and uniform heating, and at the same time, the heating efficiency is higher. The two ends of the bag body are respectively provided with an inlet pipe and an outlet pipe for the inlet and outlet of the liquid to be heated, wherein the inlet pipe is also connected with a liquid pipe and an air pipe, when the liquid pipe is connected with the inlet pipe, it is used to inject the liquid to be preheated into the bag body, and when the air pipe is connected with the inlet pipe, it is used to drain the liquid in the bag body and the inlet pipe and the outlet pipe to avoid waste. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the contents of the embodiments of the present invention and these drawings without paying any creative work.

[0023] Figure 1 is a schematic diagram of a liquid heating and supplying device provided in a specific embodiment of the present invention;

[0024] Figure 2 is a cross-sectional view of a liquid heating and supplying device provided in a specific embodiment of the present invention;

[0025] Figure 3 It is a schematic diagram of a consumable bag of a liquid heating and supplying device provided in a specific embodiment of the present invention.

[0026] In the figure:

[0027] 100, heating body; 110, locking assembly; 120, insulation layer; 130, hinge;

[0028] 200, consumable bag; 210, bag body; 220, outlet pipe; 230, inlet pipe; 240, liquid pipe; 250, air pipe;

[0029] 300, heating assembly; 310, heating element; 320, heat conductive block. DETAILED DESCRIPTION

[0030] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0031] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance. Among them, the terms "first position" and "second position" are two different positions.

[0032] Unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0033] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0034] The technical solution of the present invention is further described below with reference to the accompanying drawings and through specific implementation methods.

[0035] like Figure 1-Figure 3 As shown, this embodiment provides a liquid heating and supply device, which includes two heating bodies 100 that can be buckled together, a consumable bag 200 and a heating assembly 300. The consumable bag 200 includes a bag body 210, an outlet pipe 220 and an inlet pipe 230. The outlet pipe 220 and the inlet pipe 230 are both connected to the bag body 210. The end of the inlet pipe 230 away from the bag body 210 is respectively connected to the liquid pipe 240 and the air pipe 250, and the inlet pipe 230 can selectively connect with the liquid pipe 240 and the air pipe 250; the heating assembly 300 includes a heating element 310 and a heat conductive block 320 that are arranged in a one-to-one correspondence. The heat conductive block 320 is arranged on the side of the heating body 100 that is close to each other, and the heating element 310 is arranged between the heat conductive block 320 and the heating body 100. A placement groove is arranged on the side of the heat conductive block 320 away from the heating element 310. The two placement grooves are buckled to form a placement space, and the bag body 210 can be placed in the placement space.

[0036] The consumable bag 200 is provided to hold the liquid to be preheated. The heating body 100 is provided to heat the liquid to be preheated in the bag body 210; specifically, the heating body 100 is provided with a heating element 310 and a heat-conducting block 320, wherein the heating element 310 is used to provide high temperature, and the heat-conducting block 320 is provided between the heating element 310 and the bag body 210, for uniform temperature, and reducing the failure problem of the liquid to be preheated caused by excessive local temperature; and the heat-conducting block 320 is provided with a placement groove, when the two heating bodies 100 are buckled, the two placement grooves are buckled to form a placement space that can completely cover the bag body 210, and the embracing heating can effectively ensure sufficient and uniform heating, and at the same time, the heating efficiency is higher. An inlet pipe 230 and an outlet pipe 220 are respectively provided at both ends of the bag body 210 for the entry and exit of the liquid to be heated, wherein the inlet pipe 230 is also connected to a liquid pipe 240 and an air pipe 250. When the liquid pipe 240 is connected to the inlet pipe 230, it is used to inject the liquid to be preheated into the bag body 210. When the air pipe 250 is connected to the inlet pipe 230, it is used to drain the liquid in the bag body 210 and the inlet pipe 230 and the outlet pipe 220 to avoid waste.

[0037] Exemplarily, the heating element 310 is configured as a PI heating film. The bag body 210 has a thickness of 0.1 mm to 1 mm and a volume range of 1 ml to 20 ml. Preferably, a volume of 10 ml can be selected. The bag body 210 can be made of a PP film, or a PP composite film, or can be provided with an aluminum layer. Preferably, a PP composite film with an aluminum thin layer is used.

[0038] Optionally, the inlet pipe 230, the outlet pipe 220 and the air pipe 250 may be PVC pipes, silicone pipes, PU pipes, etc. The air pipe 250 is provided with a filter, and when the air pipe 250 and the inlet pipe 230 are connected to drain the liquid, the air entering the consumable bag 200 is filtered to avoid contamination of the liquid in the bag body 210. A sensor may be provided on the outlet pipe 220 to detect whether there is liquid passing through the outlet pipe 220. The sensor may be an ultrasonic flow sensor or an optical flow sensor. In addition, a peristaltic pump is provided on the inlet pipe 230 to pump the liquid to be preheated into the bag body 210.

[0039] Specifically, one end of the two heating bodies 100 is rotatably connected to facilitate opening and closing operations. Exemplarily, the rotatable connection is achieved through a hinge 130; a locking assembly 110 is provided at the other end of the two heating bodies 100 for locking the two heating bodies 100 after the two heating bodies 100 are buckled together.

[0040] Preferably, a heat preservation layer 120 is provided on one side of the heating element 310 away from the heat conductive block 320. The heat preservation layer 120 can wrap up the heat conductive block 320, while only exposing the side of the heat conductive block 320 provided with the placement groove. After the consumable bag 200 is installed in the heat conductive block 320, the two heating bodies 100 are buckled together, so that the bag body 210 of the consumable bag 200 is basically in a completely wrapped state for heating, and the heating effect is better. The structures of the two heating bodies 100 are the same. In other embodiments, one of the heating bodies 100 can also be set as the above structure, and the other heating body 100 can be set as a metal plate with heat preservation function, a combination of heat preservation cotton or other plastic parts.

[0041] Furthermore, the heat conducting block 320 is provided with a plurality of placement slots at intervals along its length direction, which are used to heat a plurality of consumable bags 200 at the same time, thereby improving the ability of the liquid heating and supply device to supply more liquid at the same time, so as to adapt to more usage scenarios. It is understandable that the width of the placement slot is adapted to the width of the consumable bag 200 after being filled with liquid. And the plurality of placement slots are independent of each other and do not affect each other.

[0042] Preferably, the liquid heating and supply device is further provided with a temperature detection element, which is used to detect the temperature of the placement space, and by detecting the temperature of the placement space, it is used to determine the temperature of the liquid in the consumable bag 200. Optionally, the temperature detection element can be set in the heat conductive block 320 of one of the heating bodies 100. The temperature of the placement space can be set to 37°C.

[0043] In this embodiment, in order to realize that the inlet pipe 230 can be selectively connected with the liquid pipe 240 and the air pipe 250, the consumable bag 200 also includes a three-way valve, which is respectively connected to the inlet pipe 230, the liquid pipe 240 and the air pipe 250, and the liquid pipe 240 and the air pipe 250 are opened and closed by controlling the position of the valve body of the three-way valve; or the consumable bag 200 also includes two switch valves, which are respectively arranged on the liquid pipe 240 and the air pipe 250, and the liquid pipe 240 and the air pipe 250 are opened and closed by respectively controlling the opening and closing of the switch valves.

[0044] The present embodiment also discloses a liquid supply method, which adopts the liquid heating and supply device as described in any of the above schemes; the volume of liquid in the inlet pipe 230 is a, the volume of liquid in the bag body 210 is b, the volume of liquid in the outlet pipe 220 is c, and the required liquid volume is d. The liquid supply method includes: when d≤a+b+c, directly discharging the liquid with a volume of d in the consumable bag 200 through the outlet pipe 220; when d>a+b+c, first injecting a volume of 1.5 (dabc) of liquid into the consumable bag 200 through the inlet pipe 230 and heating it, and then discharging the liquid with a volume of d in the consumable bag 200 through the outlet pipe 220.

[0045] The above liquid supply method can effectively solve the problem of liquid waste caused by the volume of preheated liquid being larger than the actual required liquid volume in the prior art. Based on the structure of the liquid heating and supply device, the required liquid volume is judged and compared with the total volume of the liquid in the bag body 210, the inlet pipe 230 and the outlet pipe 220, and then the amount of liquid injected into the consumable bag 200 is controlled, so that when the liquid in the bag body 210, the inlet pipe 230 and the outlet pipe 220 can meet the liquid supply once, the liquid is directly supplied; when the liquid in the bag body 210, the inlet pipe 230 and the outlet pipe 220 cannot meet the liquid supply once, the liquid is first injected into the consumable bag 200, and then the liquid is supplied. The amount of injected liquid is set with a certain safety margin to ensure the accuracy of liquid filling.

[0046] This embodiment also discloses a cell culture device, including the liquid heating and supply device as described in any of the above schemes. The cell culture device provided with the above liquid heating and supply device can provide preheated liquid for cell liquid replacement and cell passaging, thereby reducing the occurrence of cell culture failure; at the same time, the liquid in the liquid heating and supply device can be drained, thereby reducing waste.

[0047] Specifically, the cell culture equipment is provided with a control system, and each actuator and detection component is connected to the control system in communication. The use process of the above-mentioned liquid heating and supply device is as follows: the temperature of the placement space is set to a constant temperature such as 37°C, and the consumable bag 200 is installed in the placement slot, and the heating body 100 is buckled and locked. When the injection instruction is received, the liquid pipe 240 and the inlet pipe 230 are connected, and the peristaltic pump starts to rotate, and the liquid in a bottle is extracted, and flows into the bag body 210 located in the placement space through the inlet pipe 230, and then flows out to the outlet pipe 220. When the control system determines that the current liquid filling test is the last test, it will fill the liquid according to the liquid supply method. When filling the liquid, the inlet pipe 230 and the air pipe 250 are connected, and the peristaltic pump rotates to allow air to flow into the inlet pipe 230 after passing through the filter, and then the liquid in the consumable bag 200 is filled into the corresponding container. If the remaining liquid in the consumable is less than or equal to the required filling amount, the liquid pipe 240 and the inlet pipe 230 are first connected, and the peristaltic pump rotates to extract a certain amount of liquid. After the extraction is completed, the inlet pipe 230 and the air pipe 250 are connected, and the peristaltic pump keeps rotating, and the air flows into the inlet pipe 230 through the filter. After the liquid filling is completed, all the remaining liquid can be discharged into the waste liquid area.

[0048] The above contents are only preferred embodiments of the present invention. For ordinary technicians in this field, according to the concept of the present invention, there will be changes in the specific implementation methods and application scopes. The content of this specification should not be understood as limiting the present invention.

Claims

1. Liquid heating and supply device, characterized in that: include: Two heating bodies (100) that can be buckled together; A consumables bag (200), comprising a bag body (210), an outlet pipe (220) and an inlet pipe (230), wherein the outlet pipe (220) and the inlet pipe (230) are both connected to the bag body (210), and one end of the inlet pipe (230) away from the bag body (210) is respectively connected to a liquid pipe (240) and an air pipe (250), and the inlet pipe (230) can selectively connect to the liquid pipe (240) and the air pipe (250); The heating assembly (300) comprises a heating element (310) and a heat-conducting block (320) arranged in a one-to-one correspondence, wherein the heat-conducting block (320) is arranged on the side of the heating body (100) close to each other, the heating element (310) is arranged between the heat-conducting block (320) and the heating body (100), and a placement groove is arranged on the side of the heat-conducting block (320) away from the heating element (310), and the two placement grooves are buckled to form a placement space, and the bag body (210) can be placed in the placement space.

2. The liquid heating and supplying device according to claim 1, characterized in that: One end of the two heating bodies (100) is rotatably connected, and the other end is provided with a locking assembly (110) for locking the two heating bodies (100).

3. The liquid heating and supplying device according to claim 1, characterized in that: A heat-insulating layer (120) is provided on a side of the heating element (310) away from the heat-conducting block (320).

4. The liquid heating and supplying device according to claim 1, characterized in that: The heat conducting block (320) is provided with a plurality of placement grooves at intervals along its length direction.

5. The liquid heating and supplying device according to claim 1, characterized in that: The air pipe (250) is provided with a filter.

6. The liquid heating and supplying device according to claim 1, characterized in that: The liquid heating and supplying device is also provided with a temperature detecting component, and the temperature detecting component is used to detect the temperature of the placement space.

7. The liquid heating and supplying device according to claim 1, characterized in that: The bag body (210) is made of a PP film, and the PP film is provided with an aluminum layer.

8. The liquid heating and supplying device according to claim 1, characterized in that: The consumable bag (200) further comprises a three-way valve, wherein the three-way valve is respectively connected to the inlet pipe (230), the liquid pipe (240) and the air pipe (250); or the consumable bag (200) further comprises two switch valves, wherein the two switch valves are respectively arranged on the liquid pipe (240) and the air pipe (250).

9. A liquid supply method, characterized in that: A liquid heating and supply device as claimed in any one of claims 1 to 8 is used; the volume of liquid in the inlet pipe (230) is a, the volume of liquid in the bag body (210) is b, the volume of liquid in the outlet pipe (220) is c, and the required volume of liquid is d. The liquid supply method comprises: when d≤a+b+c, directly discharging the liquid with a volume of d in the consumable bag (200) through the outlet pipe (220); when d>a+b+c, firstly injecting a volume of 1.5 (dabc) of liquid into the consumable bag (200) through the inlet pipe (230) and heating it, and then discharging the liquid with a volume of d in the consumable bag (200) through the outlet pipe (220).

10. A cell culture device, characterized in that It comprises the liquid heating and supplying device as described in any one of claims 1-8.

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