Sample acquisition method and device for water area ecological health condition analysis

Abstract

The invention discloses a sample acquisition method and device for water area ecological health condition analysis. The sample acquisition method for analyzing the ecological health condition of the water area comprises the steps that a bottom mud layer identifier of a bottom mud layer where a sample acquisition device is located is determined, the sample acquisition device comprises at least one bottom mud acquisition element and at least one gas collection element, and the bottom mud acquisition element is communicated with the gas collection element through a pipeline; according to the bottom mud layer identifier, activating a bottom mud acquisition element corresponding to the enabled sample acquisition device and acquiring a bottom mud sample; environment parameters of the bottom mud acquisition element in the activated state are obtained, the working environment of a target gas collection element is adjusted according to the environment parameters, so that the internal environment and the external environment of the bottom mud acquisition element are matched, and the target gas collection element is a gas collection element in the sample acquisition device; when the breathing gas sample acquisition condition is satisfied, the gas collection element is enabled to collect the breathing gas sample. According to the method, a more accurate bottom mud microorganism respiration sample can be obtained.

Description

Technical Field

[0001] The present application relates to the technical field of water ecological health management and control, and specifically to a sample acquisition method and equipment for analyzing the ecological health status of water bodies. Background Art

[0002] As an important part of aquatic ecosystems, the intensity of microbial respiration activity in river sediments directly reflects the ecological health of the basin. Therefore, how to obtain more accurate sediment microbial respiration samples is a technical problem that needs to be solved urgently. Summary of the invention

[0003] The embodiments of the present application provide a sample acquisition method and device for analyzing the ecological health status of aquatic areas, aiming to obtain more accurate sediment microbial respiration samples.

[0004] On the one hand, the present application provides a sample acquisition method for analyzing the health status of water area ecology, and the sample acquisition method for analyzing the health status of water area ecology comprises:

[0005] Determine a bottom mud layer identifier of a bottom mud layer where a sample acquisition device is located, wherein the sample acquisition device includes at least one bottom mud acquisition element and at least one gas collection element, and the bottom mud acquisition element is connected to the gas collection element through a pipeline;

[0006] According to the bottom mud layer identifier, enabling the bottom mud acquisition element corresponding to the sample acquisition device to be activated and acquire the bottom mud sample;

[0007] Acquire and adjust the working environment of the target gas collection element according to the environmental parameters of the activated sediment acquisition element so that the internal and external environments of the sediment acquisition element match each other, wherein the target gas collection element is a gas collection element in the sample acquisition device;

[0008] When the respiratory gas sample acquisition condition is met, the gas collecting element is enabled to collect the respiratory gas sample.

[0009] In some embodiments, after adjusting the working environment of the target gas collection element so that the internal and external environments of the sediment acquisition element match, the method further includes:

[0010] Obtaining the rate of change of gas concentration of respiratory gas;

[0011] Based on the gas concentration change rate, determining whether the sediment microorganisms in the sediment acquisition element have abnormal respiration;

[0012] If the sediment microorganisms in the sediment acquisition element do not have abnormal breathing, it is determined that the respiratory gas sample acquisition conditions are met.

[0013] In some embodiments, determining whether the sediment microorganisms in the sediment acquisition element have abnormal respiration based on the gas concentration change rate includes:

[0014] Determine a target sediment layer where the sediment acquisition element is currently located, wherein the sediment at the target river includes a plurality of candidate sediment layers, and the plurality of candidate sediment layers includes the target sediment layer;

[0015] Determine a preset change rate range corresponding to the target sediment layer;

[0016] Based on the gas concentration change rate and the preset change rate range, it is determined whether the sediment microorganisms in the sediment acquisition element have abnormal breathing.

[0017] In some embodiments, determining the bottom mud layer identifier of the bottom mud layer where the sample acquisition device is located includes:

[0018] Acquire the depth information of the bottom mud layer where the sample acquisition device is located;

[0019] Determining a candidate bottom mud layer identifier based on the depth information;

[0020] Acquire a cross-sectional image of the bottom mud layer where the sample acquisition device is located;

[0021] According to the cross-sectional image, a sediment layer identifier of the sediment layer where the sample acquisition device is located is determined from among the candidate sediment layer identifiers.

[0022] On the other hand, an embodiment of the present application provides a sample acquisition device for analyzing the ecological health status of water bodies, comprising a body and a controller, wherein the body comprises at least one sediment acquisition element and at least one gas collection element, and the sediment acquisition element is connected to the gas collection element through a pipeline;

[0023] The controller is used to: determine the sediment layer identifier of the sediment layer where the sample acquisition device is located; enable the sediment acquisition element corresponding to the sample acquisition device to be activated and acquire the sediment sample according to the sediment layer identifier; obtain and adjust the working environment of the target gas collection element according to the environmental parameters where the activated sediment acquisition element is located so that the internal and external environments of the sediment acquisition element match, and the target gas collection element is a gas collection element in the main body; and enable the gas collection element to collect the respiratory gas sample when the respiratory gas sample acquisition conditions are met.

[0024] In some embodiments, the body further comprises a gas concentration detection element, the gas concentration detection element being used to detect the gas concentration of the respiratory gas;

[0025] The controller is also used to: use the gas concentration detection element to obtain the gas concentration change rate of the respiratory gas; based on the gas concentration change rate, determine whether the sediment microorganisms in the sediment acquisition element have abnormal breathing; if the sediment microorganisms in the sediment acquisition element do not have abnormal breathing, determine that the respiratory gas sample acquisition conditions are met.

[0026] In some embodiments, the sediment acquisition element is provided with a positioning module;

[0027] The controller is also used to: use the positioning module to determine the target sediment layer where the sediment acquisition element is currently located, wherein the sediment at the target river includes multiple candidate sediment layers, and the multiple candidate sediment layers include the target sediment layer; determine a preset change rate range corresponding to the target sediment layer; and determine whether the sediment microorganisms in the sediment acquisition element have abnormal breathing based on the gas concentration change rate and the preset change rate range.

[0028] In some embodiments, the body further comprises a depth sensor and an image sensor;

[0029] The controller is also used to: use the depth sensor to obtain depth information of the mud layer where the sample acquisition device is located; determine a candidate mud layer identifier based on the depth information; use the image sensor to obtain a cross-sectional image of the mud layer where the sample acquisition device is located; and determine the mud layer identifier of the mud layer where the sample acquisition device is located from the candidate mud layer identifiers based on the cross-sectional image.

[0030] In some embodiments, the sediment acquisition element in the body includes a sediment container and a sediment cover, wherein the sediment cover is connected to the sediment container via a bearing, and the bearing is used to activate the sediment acquisition element so that the sediment cover is engaged with the sediment container.

[0031] In some embodiments, the pipeline between the sediment acquisition element and the gas collection element includes a first transmission pipe and a second transmission pipe, and the first transmission pipe and the second transmission pipe are connected by a check valve.

[0032] On the other hand, the present application also provides an electronic device, the electronic device comprising:

[0033] one or more processors;

[0034] Memory; and

[0035] One or more applications, wherein the one or more applications are stored in the memory and are configured to be executed by the processor to implement the steps in the sample acquisition method for analyzing the health status of water body ecosystems as described in any of the above items.

[0036] On the other hand, the present application also provides a computer-readable storage medium having a computer program stored thereon, wherein the computer program is loaded by a processor to execute the steps in the sample acquisition method for analyzing the ecological health status of water bodies as described in any of the above items.

[0037] The embodiment of the present application provides a sample acquisition method and device for analyzing the ecological health status of aquatic areas. The embodiment of the present application enables the activation of the sediment acquisition element and acquires the sediment sample based on the sediment layer identification of the sediment layer where the sample acquisition device is located, thereby collecting the sediment sample of the specified sediment layer, and by matching the internal and external environments of the sediment acquisition element, when the respiratory gas sample acquisition conditions are met, the gas collection element is enabled to collect the respiratory gas sample, so as to ensure the stability of the sediment microbial environment when collecting the respiratory gas sample, and avoid affecting the respiration of the sediment microorganisms, so that the collected respiratory gas sample is more in line with the actual situation. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0039] Figure 1 This is a flow chart of an embodiment of a sample acquisition method for analyzing the ecological health status of water bodies provided in an embodiment of the present application;

[0040] Figure 2 is a schematic diagram of a three-dimensional structure of a sample acquisition device provided in an embodiment of the present application;

[0041] Figure 3 is a schematic diagram of a three-dimensional structure of a sediment acquisition element provided in an embodiment of the present application;

[0042] Figure 4 It is a structural schematic diagram of a sample acquisition device for analyzing the ecological health status of water bodies provided in an embodiment of the present application;

[0043] Figure 5 It is a schematic diagram of a terminal structure of an electronic device provided in an embodiment of the present application.

[0044] Description of reference numerals:

[0045] 10. Gas collecting element; 20. Bottom sediment acquisition element; 21. Bottom sediment container; 22. Bottom sediment cover; 23. Bearing; 30. Pipeline; 31. First transmission pipe; 32. Second transmission pipe; 33. Check valve; 34. Drain pipe provided with solenoid valve; 40. Connecting part; 400. Sample acquisition equipment for analyzing the ecological health status of water bodies; 401. Main body; 402. Controller. DETAILED DESCRIPTION

[0046] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of this application.

[0047] In the description of this application, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, "plurality" means two or more, unless otherwise clearly and specifically defined.

[0048] In this application, the term "in some embodiments" is used to mean "used as an example, illustration or description". Any embodiment described in this application as "in some embodiments" is not necessarily to be construed as being more preferred or advantageous than other embodiments. In order to enable any technician in the field to implement and use the present application, the following description is given. In the following description, details are listed for the purpose of explanation. It should be understood that a person of ordinary skill in the art can recognize that the present application can be implemented without using these specific details. In other examples, well-known structures and processes will not be elaborated in detail to avoid obscuring the description of the present application with unnecessary details. Therefore, the present application is not intended to be limited to the embodiments shown, but is consistent with the widest range of structures and features disclosed in the present application.

[0049] The embodiments of the present application provide a sample acquisition method and device for analyzing the ecological health status of aquatic areas, which are described in detail below.

[0050] Reference Figure 1 In one embodiment, a sample acquisition method for analyzing the health status of water ecology includes:

[0051] 101. Determine a sediment layer identifier of a sediment layer where a sample acquisition device is located, wherein the sample acquisition device includes at least one sediment acquisition element and at least one gas collection element, and the sediment acquisition element is connected to the gas collection element through a pipeline.

[0052] In this embodiment, the sample acquisition device is a device for obtaining microbial respiratory gas samples and / or sediment samples of the sediment layer where the microorganisms are located. When using the sample acquisition device, the sample acquisition device needs to be placed in the sediment at the target river. The sediment acquisition element in the sample acquisition device is used to collect sediment samples, and the gas collection element is used to collect respiratory gas samples. In order to facilitate the collection of respiratory gas, the sediment acquisition element is connected to the gas collection element through a pipeline, so that after the sediment sample is collected in the sediment acquisition element, the respiratory gas sample generated by the respiration of the microorganisms in the sediment sample can enter the gas collection element through the pipeline to realize the collection of respiratory gas samples.

[0053] In this embodiment, the bottom mud at the target river includes a plurality of bottom mud layers, and different bottom mud layer identifiers are pre-set for different bottom mud layers to distinguish the different bottom mud layers.

[0054] In some embodiments of the present application, determining the identifier of the sediment layer where the sample acquisition device is located may include: obtaining depth information of the sediment layer where the sample acquisition device is located, and the depth information may include the depth value of the sample acquisition device in the sediment; determining the identifier of the candidate sediment layer according to the depth information (since the sediment depths at different positions of the target river are different, for example, a layer of sediment at a certain position is thicker, while the layer of sediment at another position is thinner, the sediment layers where the sample acquisition device is located at the same depth in the sediment are different, so the sediment layer where the sample acquisition device may be located can be determined based on the depth information, and the identifier of the sediment layer where the sample acquisition device may be located can be used as candidate sediment layer identification); obtaining a profile image of the sediment layer where the sample acquisition device is located, wherein the profile image may be a sonar image, an ultrasonic image, an infrared image, etc., and the profile image may be acquired by a corresponding image sensor; according to the profile image, determining the sediment layer identification of the sediment layer where the sample acquisition device is located in the candidate sediment layer identification (due to the different sediment compositions and microbial species of different sediment layers, there will be certain differences in appearance such as color and density of different sediment layers. Therefore, based on image recognition of the profile image, the sediment layer identification of the sediment layer where the sample acquisition device is located can be determined in the candidate sediment layer identification, thereby achieving accurate identification of the sediment layer where the sample acquisition device is located).

[0055] 102. According to the identifier of the sediment layer, enable the sediment acquisition element corresponding to the sample acquisition device to be activated and acquire the sediment sample.

[0056] In this embodiment, the sediment acquisition element can be activated to acquire sediment samples. Taking the sediment acquisition element including a sediment container and a sediment cover as an example, the sediment acquisition element can be activated by opening the sediment cover, and the sediment cover switches from a state of covering the sediment container to a state of not covering the sediment container, so that the sediment can enter the sediment container to collect the sediment sample. Enabling the activation of the sediment acquisition element can be achieved by controlling the bearing connected between the sediment cover and the sediment container.

[0057] In some embodiments of the present application, based on the sediment layer identification, the sediment acquisition element corresponding to the sample acquisition device is enabled to be activated and the sediment sample is acquired, which may include: comparing the sediment layer identification with a preset identification; if the sediment layer identification is consistent with the preset identification, it indicates that the sediment acquisition element has currently reached the sediment layer where the sediment sample needs to be collected, and therefore the sediment acquisition element corresponding to the sample acquisition device is enabled to be activated and the sediment sample is acquired, so that the sediment acquisition element acquires the sediment sample in the specified sediment layer.

[0058] 103. Obtain and adjust the working environment of the target gas collection element according to the environmental parameters of the activated sediment acquisition element so that the internal and external environments of the sediment acquisition element match each other. The target gas collection element is a gas collection element in the sample acquisition device.

[0059] In this embodiment, since there is a difference between the internal and external environments of the sediment acquisition element after the sediment acquisition element is activated, which affects the respiratory activity of the microorganisms in the sediment sample, the working environment of the target gas collection element can be adjusted according to the environmental parameters of the activated sediment acquisition element, so that the internal and external environments of the sediment acquisition element match, so that the microorganisms in the sediment sample in the sediment acquisition element can perform normal respiratory activities, and the respiratory gas generated during normal respiratory activities can enter the target gas collection element through the pipeline between the sediment acquisition element and the target gas collection element, thereby realizing the collection of respiratory gas samples. Among them, the environmental parameters can be, for example, pressure, temperature, etc.

[0060] In some embodiments of the present application, after adjusting the working environment of the target gas collection element so that the internal and external environments of the sediment acquisition element match, it may also include: obtaining the gas concentration change rate of the respiratory gas; determining whether the sediment microorganisms in the sediment acquisition element have abnormal breathing based on the gas concentration change rate; if the sediment microorganisms in the sediment acquisition element do not have abnormal breathing, determining that the respiratory gas sample acquisition conditions are met. It is understandable that when the microorganisms in the sediment sample do not perform normal respiratory activities, the gas concentration change rate of the microbial respiratory gas will fluctuate greatly, so it can be determined whether the sediment microorganisms in the sediment acquisition element have abnormal breathing based on the gas concentration change rate.

[0061] In a further embodiment, determining whether the sediment microorganisms in the sediment acquisition element have abnormal breathing based on the gas concentration change rate may include: determining the target sediment layer where the sediment acquisition element is currently located, wherein the sediment at the target river includes multiple sediment layers, each sediment layer is a candidate sediment layer, and the multiple candidate sediment layers include the target sediment layer; determining a preset change rate range corresponding to the target sediment layer, wherein, due to the different types of microorganisms in different candidate sediment layers, the fluctuation amplitude of the microorganisms during respiration is also different, and therefore corresponding change rate ranges can be preset for different candidate sediment layers; based on the gas concentration change rate and the preset change rate range, determining whether the sediment microorganisms in the sediment acquisition element have abnormal breathing, for example, if the gas concentration change rate is within the preset change rate range, it is determined that the sediment microorganisms in the sediment acquisition element do not have abnormal breathing, and if the gas concentration change rate is not within the preset change rate range, it is determined that the sediment microorganisms in the sediment acquisition element have abnormal breathing, thereby making the judgment of whether the sediment microorganisms have abnormal breathing more accurate.

[0062] 104. When a respiratory gas sample acquisition condition is met, enable the gas collection element to collect the respiratory gas sample.

[0063] In this embodiment, when the conditions for obtaining the respiratory gas sample are met, it indicates that the microorganisms in the sediment sample in the sediment acquisition element are performing normal breathing activities, so the gas collecting element is enabled to collect the respiratory gas sample. For example, the air inlet of the gas collecting element can be opened, so that the respiratory gas sample can enter the gas collecting element.

[0064] In the technical solution disclosed in the present embodiment, based on the sediment layer identification of the sediment layer where the sample acquisition device is located, the sediment acquisition element is enabled to activate and acquire the sediment sample, thereby collecting the sediment sample of the specified sediment layer, and by matching the internal and external environments of the sediment acquisition element, when the respiratory gas sample acquisition conditions are met, the gas collection element is enabled to collect the respiratory gas sample to ensure the stability of the sediment microbial environment when collecting the respiratory gas sample, to avoid affecting the respiration of the sediment microorganisms, so that the collected respiratory gas samples are more in line with the actual situation.

[0065] Any of the above-mentioned sample acquisition devices is described in detail below.

[0066] Reference Figure 2 and Figure 3The sample acquisition device includes a gas collecting element 10, a sediment acquisition element 20 and a pipeline 30. The gas collecting element 10 is used to store gas, for example, to store gas (such as carbon dioxide, methane, etc.) produced when microorganisms in river sediment breathe. The sediment acquisition element 20 is used to accommodate sediment samples. The pipeline 30 is used to transmit the gas produced when microorganisms in the river sediment in the sediment acquisition element 20 breathe to the gas collecting element 10, so that even if the original environmental conditions of the river sediment change later and affect the structure of the microbial community in the sediment, it will not affect the respiratory gas sample stored in the gas collection element 10. The respiratory gas sample stored in the gas collection element 10 is sent to the laboratory for testing, so that a more accurate respiration rate of the river sediment microorganisms can be measured, and based on the respiration rate, the ecological health status information of the corresponding river basin can be determined.

[0067] The sediment acquisition element 20 is used to be inserted into the sediment at the target river. The target river can be any river whose ecological health status needs to be detected, and the target river has sediment deposited therein. The sediment acquisition element 20 includes a sediment container 21 and a sediment cover 22. The size of the sediment cover 22 is adapted to the sediment container 21 so that the sediment cover 22 can cover the sediment container 21. The sediment container 21 is provided with an opening and a receiving cavity. The opening is connected to the receiving cavity. The sediment can enter the receiving cavity from the opening, so that the sediment sample is received through the receiving cavity. The bottom mud cover 22 is used to move relative to the bottom mud container 21 so that the bottom mud cover 22 and the opening are covered or not covered. For example, when the bottom mud cover 22 is located at a first position relative to the bottom mud container 21, the bottom mud cover 22 and the opening are covered. At this time, the bottom mud outside the bottom mud acquisition element 20 cannot enter the accommodating cavity, and the bottom mud sample in the accommodating cavity cannot flow to the outside of the bottom mud acquisition element 20. For example, when the bottom mud cover 22 is located at a second position relative to the bottom mud container 21 (for example Figure 2 and Figure 3 As shown in FIG, the bottom sediment cover 22 is not covered with the opening, and the bottom sediment outside the bottom sediment acquisition element 20 can enter the accommodating cavity, that is, the accommodating cavity is connected to the external environment of the bottom sediment acquisition element 20. Therefore, the bottom sediment cover 22 is designed so that the bottom sediment in the bottom sediment layer of the specified depth at the target river can enter the accommodating cavity, and the bottom sediment in the bottom sediment layer of the non-specified depth will not enter the accommodating cavity, so as to realize the collection of microbial respiration gas samples of the bottom sediment in the bottom sediment layer of the specified depth, thereby further realizing the measurement of the microbial respiration rate of the bottom sediment in the bottom sediment layer of the specified depth, so as to better evaluate the ecological health status of the target river basin.

[0068] The pipeline 30 is connected between the gas collecting element 10 and the sediment acquisition element 20. Specifically, the pipeline 30 is used to connect the internal space of the gas collecting element 10 with the internal space of the sediment acquisition element 20, so as to transmit the gas generated by the respiration of microorganisms in the sediment acquisition element 20 to the gas collecting element 10.

[0069] It can be seen that in this embodiment, the gas generated by the respiration of microorganisms in the sediment is collected in situ in the sediment at the target river, thereby avoiding the need to send the sediment sample to the laboratory to measure the respiration rate of the microorganisms, and by covering or not covering the sediment cover 22 with the opening, the gas collection of the sediment in the specified sediment layer is achieved, avoiding mixing with the sediment in other sediment layers, ensuring the original environmental conditions of the sediment and the microbial community structure, thereby making the measurement result more accurate.

[0070] In some embodiments, reference Figure 2 and Figure 3 , a plurality of sediment acquisition elements 20 are provided in the sample acquisition device at the same time, and different sediment acquisition elements 20 are independent of each other. Different sediment acquisition elements 20 are generally at the same horizontal height, so as to facilitate the calculation of the moving distance of different sediment acquisition elements 20 in the depth direction of the target river. Since the sediment acquisition element 20 can realize the collection of sediment in the sediment layer of a specified depth, a plurality of different sediment acquisition elements 20 are used to respectively accommodate sediment samples in different sediment layers at the target river, so that the respiratory gas samples generated by the respiration of sediment microorganisms in different sediment layers can be collected, and the respiration rates of sediment microorganisms in different sediment layers can be distinguished. It can be seen that this embodiment takes into account the differences in microbial activities in different sediment layers in the target river, and through the one-time use of the sample acquisition device, the overall ecological health status of the target river can be more comprehensively evaluated.

[0071] In addition, refer to Figure 3 The outer walls of different sediment acquisition elements 20 can be connected by a connecting portion 40, and the connecting portion 40 is located between multiple sediment acquisition elements 20 to simultaneously support multiple sediment acquisition elements 20, ensuring the structural stability of the sediment acquisition elements 20 after entering the sediment layer, and a through hole is provided at the center of the connecting portion 40, and the through hole is passed through by the central axis of the sample acquisition device, so that the connecting portion 40 is fixed to the central axis, thereby further ensuring the stability of the structure. Generally speaking, multiple sediment acquisition elements 20 are arranged in rotational symmetry, and the rotation axis of rotational symmetry is the central axis.

[0072] In some embodiments, reference Figure 2For each sediment acquisition element 20, in order to make the gas in the sediment container 21 more easily transferred to the gas collecting element 10, the above-mentioned opening is arranged on the bottom surface of the sediment container 21, the pipeline 30 is connected to another opening on the top of the sediment container 21, and the gas collecting element 10 is arranged above the pipeline 30. In this way, the gas in the sediment acquisition element 20 can flow upward to the pipeline 30, and then flow upward to the gas collecting element 10 without applying external force to promote the gas flow.

[0073] In addition, the inner wall of the sediment container 21 and / or the outer wall of the sediment container 21 are generally streamlined, such as a hemispherical or semi-ellipsoidal shape. Taking the inner wall of the sediment container 21 as a hemispherical or semi-ellipsoidal shape as an example, the pipeline 30 is connected to the top of the hemispherical or semi-ellipsoidal shape, so that the gas in the sediment container 21 can flow upward to the pipeline 30 more easily. Taking the outer wall of the sediment container 21 as a hemispherical or semi-ellipsoidal shape as an example, the resistance of the sediment container 21 when moving in the sediment is smaller. Therefore, when the sample acquisition device is used for sample collection, the streamlined setting of the sediment container 21 and the precise operation and control of the sediment acquisition element 20 can limit the influence of the sample acquisition device on the biological community in the surrounding sediment to a range of no more than 2 meters in diameter, thereby minimizing the interference with the river ecosystem.

[0074] In some embodiments, reference Figure 2 and Figure 3 , the moving direction of the sediment cover 22 relative to the sediment container 21 is parallel to the upper surface and / or lower surface of the sediment cover 22. Specifically, the sediment cover 22 is in the shape of a flat plate as a whole, the upper surface of the sediment cover 22 is the upper surface of the flat plate, and the lower surface of the sediment cover 22 is the lower surface of the flat plate. Since when the sediment acquisition element 20 is located in the sediment at the target river, the sediment cover 22 is often subjected to a large pressure from the sediment. If the moving direction of the sediment cover 22 relative to the sediment container 21 is perpendicular to the upper surface and / or lower surface of the sediment cover 22, the movement of the sediment cover 22 will be more difficult. Therefore, by setting the moving direction of the sediment cover 22 relative to the sediment container 21 to be parallel to the upper surface and / or lower surface of the sediment cover 22, the movement of the sediment cover 22 is less difficult. Especially for the deeper sediment in the target river, the reduction in the difficulty of moving the sediment cover 22 is greater.

[0075] In some embodiments, reference Figure 3 The edge of the sediment cover 22 is connected to the bottom edge of the sediment container 21 through a bearing 23. In this way, the sediment cover 22 can be rotated relative to the sediment container 21 by controlling the bearing 23, and the rotation direction is parallel to the upper surface and / or lower surface of the sediment cover 22, thereby realizing the movement of the sediment cover 22 relative to the sediment container 21.

[0076] In some embodiments, a preset gas sensor (not shown) and a preset valve (not shown) are provided at the gas collection element 10. The preset gas sensor is used to detect the gas flow rate entering the gas collection element 10. The preset gas sensor can be, for example, a sensor for gases such as carbon dioxide and methane. The preset valve is used to close the gas inlet of the gas collection element 10 based on the gas flow rate. For example, the volume of gas that has entered the gas collection element 10 can be determined based on the gas flow rate. When the volume of gas that has entered the gas collection element 10 is greater than the preset volume, the gas inlet of the gas collection element 10 is closed by the preset valve. When the volume of gas that has entered the gas collection element 10 is less than or equal to the preset volume, the gas inlet of the gas collection element 10 is not closed, thereby ensuring that a sufficient amount of respiratory gas samples generated by the respiration of sediment microorganisms can be collected in the gas collection element 10, which is convenient for measuring the respiration rate of microorganisms based on these respiratory gas samples. In addition, the preset valve can be a quick closing valve to minimize the escape of gas samples. The gas inlet of the gas collection element 10 is generally arranged at the bottom of the gas collection element 10.

[0077] In some embodiments, the pipeline 30 includes a gas transmission pipe and a pressure regulating module (not shown), the gas transmission pipe is used to connect the gas collection element 10 with the sediment acquisition element 20, and the pressure regulating module is used to adjust the working environment of the target gas collection element based on the external environmental parameters of the sediment acquisition element 20 so that the internal and external environments of the sediment acquisition element 20 match, for example, to keep the internal and external environmental pressures of the sediment acquisition element 20 consistent. It should be noted that due to changes in factors such as the sediment depth and flow rate in the target river, the external environmental parameters of the sediment acquisition element 20 will also change accordingly. Therefore, through the pressure regulation of the pressure regulating module, it is possible to avoid environmental changes affecting the respiration of sediment microorganisms in the sediment acquisition element 20, thereby ensuring the reliability of the collection of respiratory gas samples.

[0078] In some embodiments, reference Figure 2The gas transmission pipe may include a first transmission pipe 31, a second transmission pipe 32, a check valve 33, and a drain pipe 34 provided with a solenoid valve. The first transmission pipe 31 is generally arranged in a horizontal direction, and the above-mentioned other openings of different sediment acquisition elements 20 are connected to different first transmission pipes 31, and different first transmission pipes 31 are not connected to each other. The second transmission pipe 32 is generally arranged in a vertical direction and is connected to the first transmission pipe 31. The check valve 33 is arranged at the second transmission pipe 32, and is used to limit the flow direction of the gas and / or liquid in the second transmission pipe 32, so that the flow direction of the gas and / or liquid in the second transmission pipe 32 can only be from bottom to top, but not from top to bottom. The drain pipe 34 provided with a solenoid valve is connected to the second transmission pipe 32, and is used to limit the flow direction of the gas and / or liquid in the second transmission pipe 32, so that the gas and / or liquid in the second transmission pipe 32 can only be discharged from the drain pipe 34 provided with a solenoid valve, and the external air cannot enter the second transmission pipe 32 through the drain pipe 34 provided with a solenoid valve. In addition, the second transmission tube 32 is generally coaxially disposed with the central axis of the sample acquisition device and fixed to the central axis of the sample acquisition device to ensure the structural stability of the sample acquisition device.

[0079] In some embodiments, the sample acquisition device further includes a displacement execution module (not shown), which may include a drive motor, for example. The displacement execution module is used to move the sediment acquisition element 20 downward according to a preset depth value so that the sediment acquisition element 20 reaches a specified sediment layer in the target river, and then controls the bearing 23 to move the sediment cover 22 relative to the sediment container 21 so that the sediment cover 22 does not cover the opening, so that the sediment of the specified sediment layer in the target river will enter the sediment acquisition element 20, and the gas generated by the sediment microorganisms in the sediment acquisition element 20 during breathing can automatically flow to the gas collection element 10, thereby realizing the automatic collection of the gas generated by the sediment microorganisms during breathing, and improving the efficiency and accuracy of the collection of respiratory gas samples.

[0080] In some embodiments of the present application, the operation process of the sample acquisition device is described by way of example, which may specifically include: controlling the sediment acquisition element 20 to move downward according to a preset depth value; controlling the sediment cover 22 to move relative to the sediment container 21 so that the sediment cover 22 does not cover the opening of the sediment container 21; and detecting the gas flow entering the gas collection element 10.

[0081] In this embodiment, the preset depth value can be pre-set based on actual needs. For example, the sediment stratification at the target river can be investigated in advance to determine the number of sediment layers and the thickness of each sediment layer at the target river, and then the depth of the specified sediment layer can be determined to obtain the preset depth value.

[0082] In this embodiment, before the sediment acquisition element 20 is controlled to move downward according to the preset depth value, the sediment cover 22 is covered with the opening of the sediment container 21 to prevent the sediment of other sediment layers in the target river from entering the sediment acquisition element 20, and after the sediment acquisition element 20 is controlled to move downward according to the preset depth value, the sediment cover 22 can be controlled not to cover the opening of the sediment container 21, so that the sediment of the designated sediment layer in the target river can enter the sediment acquisition element 20. After the sediment enters the sediment acquisition element 20, the gas generated by the respiration of the microorganisms in the sediment can flow to the gas collection element 10 through the pipeline 30, so the gas flow entering the gas collection element 10 can be detected by the preset gas sensor.

[0083] In this embodiment, based on the gas flow rate, the gas inlet of the gas collecting element 10 is closed by using a preset valve. For example, the volume of gas that has entered the gas collecting element 10 can be determined based on the gas flow rate. When the volume of gas that has entered the gas collecting element 10 is greater than the preset volume, the gas inlet of the gas collecting element 10 is closed by using a preset valve. When the volume of gas that has entered the gas collecting element 10 is less than or equal to the preset volume, the gas inlet of the gas collecting element 10 is not closed, thereby ensuring that a sufficient amount of gas generated by the respiration of sediment microorganisms can be collected in the gas collecting element 10, thereby facilitating the measurement of the microbial respiration rate based on these gases.

[0084] In some embodiments, after closing the gas inlet of the gas collecting element 10, it can also include: controlling the movement of the sediment cover 22 relative to the sediment container 21 so that the sediment cover 22 covers the opening of the sediment container 21, thereby retaining the sediment sample in the sediment acquisition element 20, so that when the measurement result based on the gas in the gas collecting element 10 is abnormal, the measurement result can be verified based on the sediment sample retained in the sediment acquisition element 20.

[0085] In some embodiments, when a plurality of sediment acquisition elements 20 are provided in the sample acquisition device at the same time, the above-mentioned preset depth values ​​may also be provided at the same time, and different preset depth values ​​correspond to different sediment layers in the target river. The moving direction of the sediment acquisition element 20 is generally vertically downward to ensure the consistency of the sediment collection direction. When a plurality of preset depth values ​​are provided at the same time, the above steps are performed in sequence according to the order in which the plurality of preset depth values ​​are reached from the smallest, so as to collect the sediment in the plurality of different sediment layers in the target river and the gas generated by the respiration of the sediment microorganisms in sequence, so as to realize the layered collection of the plurality of different sediment layers in the target river.

[0086] In the technical solution disclosed in this embodiment, the gas generated by the respiration of microorganisms in the sediment is collected in situ in the sediment at the target river, thereby avoiding the need to send the sediment sample to the laboratory to measure the respiration rate of the microorganisms, and by covering or not covering the sediment cover 22 with the opening, the gas collection of the sediment in the specified sediment layer is achieved, avoiding mixing with the sediment in other sediment layers, ensuring the original environmental conditions of the sediment and the microbial community structure, thereby making the measurement result more accurate.

[0087] In some embodiments, the operation process of any of the above sample acquisition devices is described again by way of example, which may specifically include the following contents:

[0088] (1) Preparation and insertion of the device: Before inserting the sample acquisition device at the target river, the gas collection element 10, the pipeline 30 and each sediment acquisition element 20 are filled with water so that the inside is filled with distilled water to simulate the pressure conditions in the sediment and reduce the gas dissolution during gas collection. Then, the sample acquisition device is inserted into the riverbed of the target river in a vertical direction, and multiple sediment acquisition elements 20 are moved to the uppermost sediment according to the minimum preset depth value. At this time, the sediment cover 22 of one of the sediment acquisition elements 20 is controlled to move so that the sediment cover 22 of the sediment acquisition element 20 is not covered with the opening, while the sediment covers 22 of the other sediment acquisition elements 20 and the openings are kept covered to prevent the uppermost sediment from entering the other sediment acquisition elements 20, thereby ensuring the purity of the collected sediment.

[0089] (2) Pressure regulation and gas collection: Considering that the target river sediment pressure may change with depth and flow rate, the pressure regulation module is used to automatically adjust the working environment of the target gas collection element in real time to match the internal and external environments of the sediment acquisition element 20. In order to collect a sufficient amount of gas generated by the respiration of sediment microorganisms, a preset gas sensor can be used to monitor the accumulation process of gas in the gas collection element 10, thereby accumulating measurable gas samples.

[0090] (3) Removal of gas samples: When a sufficient amount of gas has accumulated in the gas collection element 10, the preset valve can be used to close the gas inlet of the gas collection element 10 based on the gas flow rate to minimize the escape of the gas sample. The gas collection element 10 that has collected a sufficient amount of respiratory gas is then immediately transferred to a container with standard atmospheric pressure and standard temperature control to ensure the stability of the respiratory gas sample during transportation. After the respiratory gas is transported to the laboratory, the respiratory gas sample can be analyzed in detail using gas chromatography or other suitable analytical techniques to determine the corresponding chemical indicators of microbial activity, such as the concentration of gases such as carbon dioxide and methane, so as to infer the microbial respiration rate in the corresponding sediment.

[0091] (4) Sediment stratification collection process: After collecting the top sediment layer and the respiratory gas samples generated by the respiration of microorganisms in the top sediment layer, the sediment cover 22 of the sediment acquisition element 20 that collected the top sediment layer is controlled to move so that the sediment cover 22 of the sediment acquisition element 20 is covered with the opening to retain the sediment sample therein. Then, multiple sediment acquisition elements 20 are moved to the next layer of sediment of the top sediment layer, that is, the second layer of sediment, and the sediment cover 22 of the sediment acquisition element 20 corresponding to the second layer of sediment is controlled to move so that the sediment cover 22 of the sediment acquisition element 20 is not covered with the opening, and a new gas collection element 10 is replaced as the target gas collection element, and the above-mentioned gas collection process is repeated. At this time, the sediment covers 22 of other sediment acquisition elements 20 and the openings are kept covered to prevent the second layer of sediment from entering the other sediment acquisition elements 20, thereby ensuring the purity of the collected sediment. After all layers of sediment and the gases produced by the respiration of sediment microorganisms are collected, multiple sediment acquisition elements 20 can be moved to the riverbed of the target river to complete the collection of respiratory gas samples. At this time, different sediment acquisition elements 20 contain sediment at different depths, corresponding to different sediment layers at the target river.

[0092] (5) Environmental monitoring module: The sample acquisition device may also include an environmental detection module, which is used to detect the external environmental parameters of each sediment acquisition element 20 during the gas collection process. The environmental detection module may include, for example, a sensor for at least one of water temperature, water pressure, dissolved oxygen, and pH value. Such external environmental information is very important for explaining the changes in the activity of sediment microorganisms, so the microbial respiration rate may be affected by these environmental factors.

[0093] (6) Positioning module: The sample acquisition device may also include a positioning module, which is used to determine the precise position of each sediment acquisition element 20 in the sediment using technologies such as GPS or underwater sonar, so that each sediment acquisition element 20 can accurately reach the sediment at a corresponding depth. It can be seen that the positioning module can ensure the spatial continuity of sample collection, especially in a river environment with fast flow and deep water.

[0094] (7) Sediment sample isolation and protection mechanism: Each sediment acquisition element 20 is designed with an independent sealing mechanism to isolate the sediment in the sediment acquisition element 20 from the external environment and maintain the environmental parameter conditions in the sediment acquisition element 20 to prevent the sediment in the sediment acquisition element 20 from being affected by subsequent operations and environmental changes. In addition, the inner wall of each sediment acquisition element 20 is coated with an anti-biological attachment material, such as an anti-fouling polymer, a silane-modified polymer, a polymer surface co-constructed polymer, nanoparticles, etc., to prevent microorganisms from growing after attaching to the inner wall of the sediment acquisition element 20, thereby ensuring the original microbial composition in the sediment sample.

[0095] In this embodiment, the sediment samples and breath gas samples are collected in layers under the in-situ conditions of the target river, so that the microbial respiration rate in each layer of sediment can be measured separately. This real-time gas collection method directly carried out in the field not only avoids the influence of changes in environmental conditions on the microbial respiration rate, but also avoids the mixing of sediments at different levels, greatly improving the accuracy and ecological value of the measurement results. In addition, the movement of the pressure regulating module and the sediment cover 22 relative to the sediment container 21 ensures the reliability and sample integrity of the breath gas samples and sediment samples during the collection process.

[0096] In some embodiments, the sample acquisition device is also adaptable to different water qualities. Specifically, the design of the sample acquisition device takes into account stable operation under different water quality conditions with a pH value ranging from 4.5 to 9.0, and components such as the gas collection element 10, the bottom mud acquisition element 20, and the pipeline 30 in the sample acquisition device can be made of anti-corrosion materials to ensure durability in highly mineralized water bodies (e.g., salinity up to 35‰).

[0097] In some embodiments, the sample acquisition device is also adaptable to changes in water temperature. Specifically, the design of the sample acquisition device takes into account normal operation in a water temperature range of 0°C to 40°C. The gas collection element 10, the sediment acquisition element 20, the pipeline 30 and other components in the sample acquisition device can be made of thermally stable materials to ensure that the performance is not affected by temperature fluctuations.

[0098] In some embodiments, the sample acquisition device also has adaptability to changes in flow rate. Specifically, the design of the sample acquisition device takes into account normal operation under flow conditions of 0 to 5 meters per second. The sediment acquisition element 20 adopts a streamlined design to reduce water flow resistance, and is equipped with a weight adjustment module to ensure stability under high flow conditions. The weight adjustment module can automatically adjust the load of the sediment acquisition element 20 based on the flow rate of the target river (for example, it can be achieved by adjusting the weight and number of dumbbell pieces loaded by the sediment acquisition element 20), thereby ensuring the stability of the sediment acquisition element 20.

[0099] In some embodiments, the sample acquisition device is also adaptable to different depths and different sediment types. Specifically, the sample acquisition device is suitable for target rivers from shallow water areas (0.5 meter depth) to deep water areas (maximum 30 meter depth). The sediment acquisition element 20 can effectively handle different sediment types from fine-grained sand to coarse-grained gravel, ensuring effective sampling in a variety of bottom environments.

[0100] In some embodiments, the sample acquisition device is also adaptable to different sediment particle sizes. Specifically, the sample acquisition device can use a sediment acquisition element 20 made of materials with different hardness, and the hardness of the material of the sediment acquisition element 20 can be adaptively adjusted based on the sediment particle size (from fine particles with a diameter of less than 0.06 mm to coarse particles with a diameter of more than 2 mm) to ensure that the sediment acquisition element 20 is effectively inserted into sediments of different particle sizes.

[0101] In some embodiments, the sample acquisition device is also provided with a gas concentration detection element, which is used to determine the sampling accuracy in the bottom mud within a set depth range. Specifically, the sample acquisition device can adjust the sampling depth from the surface bottom mud to the bottom mud with a maximum depth of 50 cm to meet the gas sampling requirements at different depths, and the gas concentration detection element can detect the gas concentration of the respiratory gas (such as the concentration of carbon dioxide, methane and other gases) when the bottom mud microorganisms in the bottom mud acquisition element 20 breathe, and when the rate of change of the gas concentration is greater than the preset rate, it is considered that the gas sampling fluctuates greatly and the gas sampling is not accurate enough, and the corresponding prompt information can be output to the relevant operator, thereby ensuring that the accuracy of the gas sampling maintains a small error range within the set depth range, and ensuring the reliability and accuracy of the sampling of the respiratory gas sample.

[0102] In order to better implement the sample acquisition method for analyzing the ecological health status of water bodies in the embodiments of the present application, based on the sample acquisition method for analyzing the ecological health status of water bodies, a sample acquisition device 400 for analyzing the ecological health status of water bodies is also provided in the embodiments of the present application.

[0103] Reference Figure 4 The sample acquisition device 400 for analyzing the health status of water ecology includes a body 401 and a controller 402. The body 401 includes any of the above-mentioned sample acquisition devices, and the sample acquisition device includes at least one sediment acquisition element 20 and at least one gas collection element 10, and the sediment acquisition element 20 is connected to the gas collection element 10 through a pipeline 30.

[0104] The controller 402 is used to execute the sample acquisition method for analyzing the ecological health status of water bodies in any of the above embodiments. For example, the controller 402 is used to: determine the bottom mud layer identifier of the bottom mud layer where the sample acquisition device is located; according to the bottom mud layer identifier, enable the bottom mud acquisition element 20 corresponding to the sample acquisition device to activate and acquire bottom mud samples; obtain and adjust the working environment of the target gas collection element 10 according to the environmental parameters of the bottom mud acquisition element 20 in the activated state so that the internal and external environments of the bottom mud acquisition element 20 match, and the target gas collection element 10 is a gas collection element 10 in the body 401; when the respiratory gas sample acquisition conditions are met, enable the gas collection element 10 to collect the respiratory gas sample.

[0105] In some embodiments of the present application, the body 401 further includes a gas concentration detection element, which is used to detect the gas concentration of the respiratory gas;

[0106] The controller 402 is also used to: use the gas concentration detection element to obtain the gas concentration change rate of the respiratory gas; based on the gas concentration change rate, determine whether the sediment microorganisms in the sediment acquisition element 20 have abnormal breathing; if the sediment microorganisms in the sediment acquisition element 20 do not have abnormal breathing, determine that the conditions for obtaining the respiratory gas sample are met.

[0107] In some embodiments of the present application, the sediment acquisition element 20 is provided with a positioning module;

[0108] The controller 402 is also used to: use the positioning module to determine the target sediment layer where the sediment acquisition element 20 is currently located, wherein the sediment at the target river includes multiple candidate sediment layers, and the multiple candidate sediment layers include the target sediment layer; determine a preset change rate range corresponding to the target sediment layer; and determine whether the sediment microorganisms in the sediment acquisition element 20 have abnormal breathing based on the gas concentration change rate and the preset change rate range.

[0109] In some embodiments of the present application, the body 401 further includes a depth sensor and an image sensor;

[0110] The controller 402 is also used to: use a depth sensor to obtain depth information of the sediment layer where the sample acquisition device is located; determine a candidate sediment layer identifier based on the depth information; use an image sensor to obtain a profile image of the sediment layer where the sample acquisition device is located; and determine the sediment layer identifier of the sediment layer where the sample acquisition device is located from among the candidate sediment layer identifiers based on the profile image.

[0111] In some embodiments of the present application, the sediment acquisition element 20 in the main body 401 includes a sediment container 21 and a sediment cover 22. The sediment cover 22 is connected to the sediment container 21 via a bearing 23. The bearing 23 is used to activate the sediment acquisition element 20 so that the sediment cover 22 is covered with the sediment container 21.

[0112] In some embodiments of the present application, the pipeline 30 between the sediment acquisition element 20 and the gas collection element 10 includes a first transmission pipe 31 and a second transmission pipe 32 , and the first transmission pipe 31 and the second transmission pipe 32 are connected via a check valve 33 .

[0113] In order to better implement the sample acquisition method for analyzing the health status of water ecology in the embodiment of the present application, on the basis of the sample acquisition method for analyzing the health status of water ecology, the embodiment of the present application also provides an electronic device for executing the steps in the sample acquisition method for analyzing the health status of water ecology in any of the above embodiments. Figure 5As shown, it shows a schematic diagram of the structure of the electronic device involved in the embodiment of the present application, specifically:

[0114] The electronic device may include components such as a processor 501 with one or more processing cores, a memory 502 with one or more computer-readable storage media, a power supply 503, and an input unit 504. Those skilled in the art will appreciate that Figure 5 The electronic device structure shown in the figure is not intended to limit the electronic device, and may include more or fewer components than shown in the figure, or combine certain components, or arrange the components differently.

[0115] The processor 501 is the control center of the electronic device, and uses various interfaces and lines to connect various parts of the entire electronic device. By running or executing software programs and / or modules stored in the memory 502, and calling data stored in the memory 502, the processor 501 performs various functions of the electronic device and processes data, thereby monitoring the electronic device as a whole. Optionally, the processor 501 may include one or more processing cores; preferably, the processor 501 may integrate an application processor and a modem processor, wherein the application processor mainly processes the operating system, user interface, and application programs, and the modem processor mainly processes wireless communications. It is understandable that the above-mentioned modem processor may not be integrated into the processor 501.

[0116] The memory 502 can be used to store software programs and modules. The processor 501 executes various functional applications and data processing by running the software programs and modules stored in the memory 502. The memory 502 may mainly include a program storage area and a data storage area, wherein the program storage area may store an operating system, an application required for at least one function (such as a sound playback function, an image playback function, etc.), etc.; the data storage area may store data created according to the use of the electronic device, etc. In addition, the memory 502 may include a high-speed random access memory, and may also include a non-volatile memory, such as at least one disk storage device, a flash memory device, or other volatile solid-state storage devices. Accordingly, the memory 502 may also include a memory controller to provide the processor 501 with access to the memory 502.

[0117] The electronic device also includes a power supply 503 for supplying power to each component. Preferably, the power supply 503 can be logically connected to the processor 501 through a power management system, so as to manage charging, discharging, power consumption and other functions through the power management system. The power supply 503 can also include one or more DC or AC power supplies, recharging systems, power failure detection circuits, power converters or inverters, power status indicators and other arbitrary components.

[0118] The electronic device may further include an input unit 504, which may be used to receive input digital or character information and generate keyboard, mouse, joystick, optical or trackball signal input related to user settings and function control.

[0119] Although not shown, the electronic device may further include a display unit, etc., which will not be described in detail herein. Specifically in this embodiment, the processor 501 in the electronic device will load the executable files corresponding to the processes of one or more application programs into the memory 502 according to the following instructions, and the processor 501 will run the application programs stored in the memory 502, thereby realizing various functions, such as:

[0120] Determine the sediment layer identifier of the sediment layer where the sample acquisition device is located; according to the sediment layer identifier, enable the sediment acquisition element corresponding to the sample acquisition device to be activated and acquire the sediment sample; obtain and adjust the working environment of the target gas collection element according to the environmental parameters where the activated sediment acquisition element is located so that the internal and external environments of the sediment acquisition element match, the target gas collection element being a gas collection element in the main body; when the respiratory gas sample acquisition conditions are met, enable the gas collection element to collect the respiratory gas sample.

[0121] A person of ordinary skill in the art will appreciate that all or part of the steps in the various methods of the above embodiments may be completed by instructions, or by controlling related hardware through instructions. The instructions may be stored in a computer-readable storage medium and loaded and executed by a processor.

[0122] To this end, the embodiment of the present application provides a computer-readable storage medium, which may include: a read-only memory (ROM), a random access memory (RAM), a disk or an optical disk, etc. A computer program is stored thereon, and the computer program is loaded by a processor to execute the steps in any of the sample acquisition methods for analyzing the health status of water ecosystems provided in the embodiment of the present application. For example, the computer program can be loaded by a processor to execute the following steps:

[0123] Determine the sediment layer identifier of the sediment layer where the sample acquisition device is located; according to the sediment layer identifier, enable the sediment acquisition element corresponding to the sample acquisition device to be activated and acquire the sediment sample; obtain and adjust the working environment of the target gas collection element according to the environmental parameters where the activated sediment acquisition element is located so that the internal and external environments of the sediment acquisition element match, the target gas collection element being a gas collection element in the main body; when the respiratory gas sample acquisition conditions are met, enable the gas collection element to collect the respiratory gas sample.

[0124] In the above embodiments, the description of each embodiment has its own emphasis. For parts that are not described in detail in a certain embodiment, please refer to the detailed description of other embodiments above, and will not be repeated here.

[0125] In specific implementation, the above units or structures can be implemented as independent entities, or can be arbitrarily combined to be implemented as the same or several entities. The specific implementation of the above units or structures can refer to the previous method embodiments, which will not be repeated here.

[0126] The specific implementation of the above operations can be found in the previous embodiments, which will not be described in detail here.

[0127] The above is a detailed introduction to a sample acquisition method and device for analyzing the ecological health status of aquatic areas provided in an embodiment of the present application. This article uses specific examples to illustrate the structure and implementation methods of the present application. The description of the above embodiments is only used to help understand the method of the present application and its core idea; at the same time, for technical personnel in this field, based on the ideas of the present application, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as a limitation on the present application.

Claims

1. A sample acquisition method for analyzing the ecological health status of water bodies, characterized in that: The method comprises: Determine a bottom mud layer identifier of a bottom mud layer where a sample acquisition device is located, wherein the sample acquisition device includes at least one bottom mud acquisition element and at least one gas collection element, and the bottom mud acquisition element is connected to the gas collection element through a pipeline; According to the bottom mud layer identifier, enabling the bottom mud acquisition element corresponding to the sample acquisition device to be activated and acquire the bottom mud sample; Acquire and adjust the working environment of the target gas collection element according to the environmental parameters of the activated sediment acquisition element so that the internal and external environments of the sediment acquisition element match each other, wherein the target gas collection element is a gas collection element in the sample acquisition device; When the respiratory gas sample acquisition condition is met, the gas collecting element is enabled to collect the respiratory gas sample.

2. The sample acquisition method for analyzing the ecological health status of water bodies according to claim 1, characterized in that: After adjusting the working environment of the target gas collection element so that the internal and external environments of the bottom mud acquisition element match, the method further includes: Obtaining the rate of change of gas concentration of respiratory gas; Based on the gas concentration change rate, determining whether the sediment microorganisms in the sediment acquisition element have abnormal respiration; If the sediment microorganisms in the sediment acquisition element do not have abnormal breathing, it is determined that the respiratory gas sample acquisition conditions are met.

3. The sample acquisition method for analyzing the ecological health status of water bodies according to claim 2, characterized in that: The determining whether the sediment microorganisms in the sediment acquisition element have abnormal respiration based on the gas concentration change rate includes: Determine a target sediment layer where the sediment acquisition element is currently located, wherein the sediment at the target river includes a plurality of candidate sediment layers, and the plurality of candidate sediment layers includes the target sediment layer; Determine a preset change rate range corresponding to the target sediment layer; Based on the gas concentration change rate and the preset change rate range, it is determined whether the sediment microorganisms in the sediment acquisition element have abnormal breathing.

4. The sample acquisition method for analyzing the ecological health status of water bodies according to claim 1, characterized in that: Determining the bottom mud layer identifier of the bottom mud layer where the sample acquisition device is located includes: Acquire the depth information of the bottom mud layer where the sample acquisition device is located; Determining a candidate bottom mud layer identifier based on the depth information; Acquire a cross-sectional image of the bottom mud layer where the sample acquisition device is located; According to the cross-sectional image, a sediment layer identifier of the sediment layer where the sample acquisition device is located is determined from among the candidate sediment layer identifiers.

5. A sample acquisition device for analyzing the ecological health status of water bodies, characterized in that: It comprises a body and a controller, wherein the body comprises at least one sediment acquisition element and at least one gas collection element, and the sediment acquisition element is connected with the gas collection element through a pipeline; The controller is used to: determine the sediment layer identifier of the sediment layer where the sample acquisition device is located; enable the sediment acquisition element corresponding to the sample acquisition device to be activated and acquire the sediment sample according to the sediment layer identifier; obtain and adjust the working environment of the target gas collection element according to the environmental parameters where the activated sediment acquisition element is located so that the internal and external environments of the sediment acquisition element match, and the target gas collection element is a gas collection element in the main body; and enable the gas collection element to collect the respiratory gas sample when the respiratory gas sample acquisition conditions are met.

6. The sample acquisition device for analyzing the ecological health status of water bodies according to claim 5, characterized in that: The body further comprises a gas concentration detection element, wherein the gas concentration detection element is used to detect the gas concentration of the respiratory gas; The controller is also used to: use the gas concentration detection element to obtain the gas concentration change rate of the respiratory gas; based on the gas concentration change rate, determine whether the sediment microorganisms in the sediment acquisition element have abnormal breathing; if the sediment microorganisms in the sediment acquisition element do not have abnormal breathing, determine that the respiratory gas sample acquisition conditions are met.

7. The sample acquisition device for analyzing the ecological health status of water bodies according to claim 6, characterized in that: The bottom mud acquisition element is provided with a positioning module; The controller is also used to: use the positioning module to determine the target sediment layer where the sediment acquisition element is currently located, wherein the sediment at the target river includes multiple candidate sediment layers, and the multiple candidate sediment layers include the target sediment layer; determine a preset change rate range corresponding to the target sediment layer; and determine whether the sediment microorganisms in the sediment acquisition element have abnormal breathing based on the gas concentration change rate and the preset change rate range.

8. The sample acquisition device for analyzing the ecological health status of water bodies according to claim 5, characterized in that: The body also includes a depth sensor and an image sensor; The controller is also used to: use the depth sensor to obtain depth information of the mud layer where the sample acquisition device is located; determine a candidate mud layer identifier based on the depth information; use the image sensor to obtain a cross-sectional image of the mud layer where the sample acquisition device is located; and determine the mud layer identifier of the mud layer where the sample acquisition device is located from the candidate mud layer identifiers based on the cross-sectional image.

9. The method for obtaining a sample for analyzing the health status of water ecosystems according to claim 5, characterized in that: The sediment acquisition element in the body includes a sediment container and a sediment cover, wherein the sediment cover is connected to the sediment container via a bearing, and the bearing is used to activate the sediment acquisition element so that the sediment cover is engaged with the sediment container.

10. The sample acquisition device for analyzing the ecological health status of water bodies according to claim 5, characterized in that: The pipeline between the bottom mud acquisition element and the gas collection element includes a first transmission pipe and a second transmission pipe, and the first transmission pipe and the second transmission pipe are connected through a check valve.

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