Walnut germination accelerator, preparation method thereof, method for promoting germination of walnut seeds and application of walnut germination accelerator

Abstract

The invention relates to a walnut germination accelerant, a preparation method thereof, a method for promoting walnut seed germination and application thereof, and belongs to the technical field of fruit tree cultivation. The walnut germination accelerator comprises the following components: gibberellin, naphthylacetic acid, triacontanol, indolebutyric acid and water. The method for promoting germination of the walnut seeds comprises the following steps: soaking the walnut seeds in warm water, drying, soaking the walnut seeds in a walnut germination accelerant, airing and sowing. The walnut germination promoter and the method for promoting walnut seed germination can improve the germination rate and the seedling rate of walnut seeds, and have the advantages of high safety, strong stability and good effectiveness.

Description

Technical Field

[0001] The invention relates to the technical field of fruit tree cultivation, and in particular to a walnut germination promoter and a preparation method thereof, and a method for promoting walnut seed germination and an application thereof. Background Art

[0002] Walnut, also known as walnut (Juglans regia), is a tall deciduous tree of the genus Juglans in the family Juglandaceae. Its wood is solid and is a good hardwood material. Walnut leaves are often used fresh as medicine. They are mild in nature, bitter and astringent in taste, and can astringe and stop leucorrhea, kill insects and reduce swelling. The fruit is also called walnut. It has a high oil content and can be eaten raw or pressed for oil. It also has certain medicinal value. The walnut kernel is warm in nature and mild in taste. It belongs to the kidney meridian, lung meridian and large intestine meridian. It is mainly used to treat kidney deficiency cough, back pain and weak legs, and can nourish the kidney and consolidate essence, warm the lungs and stop asthma; the walnut skin is cool in nature, astringent and slightly bitter in taste, and has the effects of clearing away heat and detoxification, killing insects and relieving itching.

[0003] Seed germination is an important part of walnut seedling cultivation. Traditional germination techniques include low-temperature stratification germination, spring watering germination, water immersion and sun germination, immersion germination, direct autumn sowing germination, etc. Although the operation is simple, there are defects such as long germination time, low walnut seed germination rate, insufficient uniformity, poor controllability, etc. At the same time, the prior art has not yet disclosed a preparation that is safe, stable and effective and can improve the germination rate and seedling rate of walnut seeds. Summary of the invention

[0004] The purpose of the present invention is to provide a walnut germination promoter and a preparation method thereof, a method for promoting walnut seed germination and an application thereof, so as to solve the problems in the prior art of long walnut germination period, poor safety, low germination rate and seedling rate of walnut seeds.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides a walnut germination promoter, comprising the following components: gibberellin, naphthylacetic acid, triacontanol, indolebutyric acid and water;

[0007] The mass volume ratio of the gibberellin to water is 30-80 mg:1 L;

[0008] The mass volume ratio of naphthylacetic acid to water is 5-15 mg:1 L;

[0009] The volume ratio of triacontanol to water is 1.5-5 mL:1 L;

[0010] The mass volume ratio of the indolebutyric acid and water is 55-75 mg:1L.

[0011] The invention also provides application of the walnut germination promoter in promoting germination of walnut seeds.

[0012] The invention also provides a preparation method of the walnut germination promoter, comprising the following steps: mixing gibberellin, naphthylacetic acid, triacontanol, indolebutyric acid and water to obtain the walnut germination promoter.

[0013] The present invention also provides a method for promoting germination of walnut seeds, comprising the following steps:

[0014] (1) soaking walnut seeds in warm water for 12 to 24 hours and drying to obtain pretreated seeds;

[0015] (2) soaking the pretreated seeds in a walnut germination promoter for 24 to 48 hours, and drying them in the sun to obtain germinated seeds;

[0016] (3) sowing the germinated seeds;

[0017] The walnut germination promoter is the walnut germination promoter described above.

[0018] Preferably, the temperature of the warm water in step (1) is 50-70°C.

[0019] Preferably, the drying temperature in step (1) is 55-75° C., and the drying time is 3-6 hours.

[0020] Preferably, the drying temperature in step (2) is 30-40° C., and the drying time is 2-5 days.

[0021] The invention also provides application of the method in promoting germination of walnut seeds.

[0022] The present invention has the following technical effects and advantages:

[0023] In the walnut germination promoter of the present invention, gibberellin is an endogenous plant hormone commonly present in plants, which can break the dormancy of walnut seeds, promote germination and the nutritional growth of walnut seedlings; naphthylacetic acid has the action characteristics and physiological functions of endogenous auxin, can promote cell division and expansion, and induce rooting of walnut seedlings; triacontanol is a natural ingredient present in plant wax in the form of ester, which is non-toxic and non-irritating, and can promote walnut seed germination, improve the growth, differentiation and development of walnut seedlings; indolebutyric acid is a plant taproot growth promoter, which can improve the germination rate and survival rate of walnut seeds, accelerate the root formation of walnut seedlings, and naphthylacetic acid and indolebutyric acid have a synergistic effect in promoting the germination and seedling formation of walnut seeds. The walnut germination promoter of the present invention has the advantages of high safety, strong stability and good effectiveness;

[0024] The method for promoting germination of walnut seeds of the present invention is easy to operate, has a significant promoting effect on germination of walnut seeds, can cooperate with a walnut germination promoter to play a germination promoting role, thereby improving the germination rate and seedling rate of the walnut seeds, and has the advantages of short germination cycle and strong controllability. DETAILED DESCRIPTION

[0025] The invention provides a walnut germination promoter, comprising the following components: gibberellin, naphthylacetic acid, triacontanol, indolebutyric acid and water;

[0026] The mass volume ratio of the gibberellin to water is 30-80 mg:1L, preferably 40-70 mg:1L, and more preferably 55 mg:1L;

[0027] The mass volume ratio of naphthylacetic acid to water is 5-15 mg:1L, preferably 8-12 mg:1L, and more preferably 10 mg:1L;

[0028] The volume ratio of triacontanol to water is 1.5-5 mL:1 L, preferably 2-4 mL:1 L, and more preferably 2.5 mL:1 L;

[0029] The mass volume ratio of indolebutyric acid to water is 55-75 mg:1L, preferably 60-70 mg:1L, and more preferably 65 mg:1L.

[0030] In the present invention, the gibberellin (C 19 H 22 O6) with an effective substance content of ≥ 90%, purchased from Hubei Baoling Chemical Technology Co., Ltd.;

[0031] The naphthylacetic acid (C 12 H 10 O2) with an effective substance content of ≥99%, purchased from Wuhan Huaxiang Kejie Biotechnology Co., Ltd.;

[0032] The triacontanol (C 30 H 62 O) with an effective substance content of ≥ 98%, purchased from Hubei Keji Biopharmaceutical Technology Co., Ltd.;

[0033] The indolebutyric acid (C 12 H 13 NO2) with an effective substance content of ≥99% was purchased from Hubei Xinyuhong Biomedicine Technology Co., Ltd.

[0034] The invention also provides application of the walnut germination promoter in promoting germination of walnut seeds.

[0035] The invention also provides a preparation method of the walnut germination promoter, comprising the following steps: mixing gibberellin, naphthylacetic acid, triacontanol, indolebutyric acid and water to obtain the walnut germination promoter.

[0036] In the present invention, the preparation method comprises the following steps: firstly mixing and dissolving gibberellins, naphthylacetic acid, indolebutyric acid and water, then adding triacontanol and mixing evenly to obtain a walnut germination promoter.

[0037] The present invention also provides a method for promoting germination of walnut seeds, comprising the following steps:

[0038] (1) soaking walnut seeds in warm water for 12 to 24 hours, preferably 18 hours, and drying to obtain pretreated seeds;

[0039] (2) soaking the pretreated seeds in a walnut germination promoter for 24 to 48 hours, preferably 36 hours, and drying them in the sun to obtain germinated seeds;

[0040] (3) sowing the germinated seeds;

[0041] The walnut germination promoter is the walnut germination promoter described above.

[0042] In the present invention, the temperature of the warm water in step (1) is 50-70°C, preferably 65°C.

[0043] In the present invention, the drying temperature in step (1) is 55-75° C., preferably 60° C.; the drying time is 3-6 hours, preferably 4 hours.

[0044] In the present invention, the drying temperature in step (2) is 30-40° C., preferably 35° C.; the drying time is 2-5 days, preferably 4 days.

[0045] The invention also provides application of the method in promoting germination of walnut seeds.

[0046] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0047] The greenhouse in the embodiment of the present invention is located in the arboretum of the Forestry Science Research Institute of Enshi Tujia and Miao Autonomous Prefecture, Enshi City, Hubei Province;

[0048] In the embodiment of the present invention, the variety of walnut is fragrant walnut, which is purchased from the Fruit Research Institute of Shanxi Agricultural University;

[0049] In the embodiments of the present invention, gibberellin was purchased from Hubei Baoling Chemical Technology Co., Ltd., naphthylacetic acid was purchased from Wuhan Huaxiang Kejie Biotechnology Co., Ltd., triacontanol was purchased from Hubei Keji Biomedical Technology Co., Ltd., and indolebutyric acid was purchased from Hubei Xinyuhong Biomedical Technology Co., Ltd.

[0050] Example 1: Preparation of walnut germination promoter

[0051] 55 mg of gibberellin, 12 mg of naphthaleneacetic acid, 60 mg of indolebutyric acid and 1 L of water were mixed and dissolved, and then 1.5 mL of triacontanol was added and mixed evenly to obtain a walnut germination promoter.

[0052] Example 2: Preparation of walnut germination promoter

[0053] 30 mg of gibberellin, 8 mg of naphthaleneacetic acid, 65 mg of indolebutyric acid and 1 L of water were mixed and dissolved, and then 2 mL of triacontanol was added and mixed evenly to obtain a walnut germination promoter.

[0054] Example 3: Preparation of walnut germination promoter

[0055] 40 mg of gibberellin, 10 mg of naphthaleneacetic acid, 70 mg of indolebutyric acid and 1 L of water were mixed and dissolved, and then 2.5 mL of triacontanol was added and mixed evenly to obtain a walnut germination promoter.

[0056] Example 4: Preparation of walnut germination promoter

[0057] 70 mg of gibberellin, 15 mg of naphthaleneacetic acid, 55 mg of indolebutyric acid and 1 L of water were mixed and dissolved, and then 4 mL of triacontanol was added and mixed evenly to obtain a walnut germination promoter.

[0058] Example 5: Germination and cultivation of walnut seeds

[0059] Walnut seeds were soaked in 65°C warm water for 18 hours, dried at 60°C for 4 hours until completely dried, and then transferred to the walnut germination promoter described in Example 1 for soaking for 36 hours, and dried at 35°C for 4 days to obtain germinated seeds; the germinated seeds were sown in a greenhouse to cultivate walnut seedlings. The management of the greenhouse was carried out according to conventional management methods.

[0060] Example 6: Germination and cultivation of walnut seeds

[0061] Walnut seeds were soaked in 50°C warm water for 24 hours, dried at 55°C for 6 hours until completely dried, and then transferred to the walnut germination promoter described in Example 2 for soaking for 24 hours, and dried at 30°C for 5 days to obtain germinated seeds; the germinated seeds were sown in a greenhouse to cultivate walnut seedlings. The management of the greenhouse was carried out according to conventional management methods.

[0062] Comparative Example 1: Germination and cultivation of walnut seeds

[0063] 55 mg of gibberellin, 60 mg of indolebutyric acid and 1 L of water were mixed and dissolved, and then 1.5 mL of triacontanol was added and mixed evenly to obtain seed soaking agent A; walnut seeds were soaked in 65°C warm water for 18 hours, dried at 60°C for 4 hours until completely dried, and then transferred to seed soaking agent A for 36 hours, and dried at 35°C for 4 days to obtain germinated seeds; the germinated seeds were sown in a greenhouse to cultivate walnut seedlings. The management of the greenhouse was carried out according to the conventional management method.

[0064] Comparative Example 2: Germination and cultivation of walnut seeds

[0065] 120 mg of gibberellin, 40 mg of indoleacetic acid, 1 mg of 6-BA and 1 L of water were mixed and dissolved to obtain soaking agent B; walnut seeds were soaked in 65°C warm water for 18 hours, dried at 60°C for 4 hours until completely dried, then transferred to soaking agent B for 36 hours, and dried at 35°C for 4 days to obtain germinated seeds; the germinated seeds were sown in a greenhouse to cultivate walnut seedlings. The management of the greenhouse was carried out according to the conventional management method.

[0066] Comparative Example 3: Germination and cultivation of walnut seeds

[0067] A 1 cm gap was opened at the tip of the walnut seed in advance, and then the seeds were soaked in the walnut germination promoter described in Example 1 for 36 hours, and then soaked in clean water for 24 hours; the walnut seeds were buried in a river sand bed with a moisture content of 15%, and the sand was stored for 7 days to obtain germinated seeds, during which the moisture content of the river sand bed was maintained at 15%; the germinated seeds were sown in a greenhouse to cultivate walnut seedlings. The management of the greenhouse was carried out in accordance with conventional management methods.

[0068] Experimental Example 1: Determination of germination rate and seedling rate of walnut seeds

[0069] 600 plump and uniformly sized fragrant walnut seeds were selected and divided into 5 groups, with 120 walnut seeds in each group; the walnut seeds in each group were germinated and cultivated according to the methods described in Examples 5 to 6 and Comparative Examples 1 to 3, respectively, the germination rate of each group of walnut seeds was counted one day before sowing, the seedling rate of each group of walnut seeds was counted on the 40th day after sowing the germinated seeds, and the average plant height and average stem height of each group of walnut seedlings were measured and calculated, and the results are shown in Table 1;

[0070] The germination rate calculation formula is:

[0071] The formula for calculating the seedling rate is:

[0072] Table 1 Germination and seedling formation of walnut seeds

[0073] Group Germination rate (%) Seedling rate (%) Average plant height (cm) Average stem height (cm) Example 5 Group 90.83 94.50 30.1 0.82 Example 6 Group 95.83 93.04 29.4 0.79 Comparative Example 1 81.67 67.35 24.3 0.66 Comparative Example 2 59.17 45.07 14.6 0.41 Comparative Example 3 46.67 64.29 16.2 0.43

[0074] The results show that the germination rate of the walnut seeds in groups 5 to 6 of embodiments is high, the germination is uniform, and the seedling rate of the walnut seedlings is high, the growth is strong and the consistency is high; the germination rate and seedling rate of the walnut seeds in group 1 of comparative example are slightly lower than those in groups 5 to 6 of embodiments, indicating that naphthylacetic acid and indolebutyric acid have a synergistic effect in promoting the germination and seedling of walnut seeds; the germination rate and germination rate of the walnut seeds in group 2 of comparative example are poor, and the growth of the walnut seedlings is weak, indicating that the seed soaking agent in the prior art has a poor effect on promoting the germination of walnut seeds; the germination rate and seedling rate of the walnut seeds in group 3 of comparative example are lower than those in groups 5 to 6 of embodiments, indicating that the method of the present invention can cooperate with the walnut germination promoter to play a germination promoting effect, and at the same time, the seedling rate and germination rate of the walnut seeds in group 3 of comparative example are higher than those of the walnut germination promoter, indicating that the walnut germination promoter of the present invention itself has strong effectiveness and stability.

[0075] It can be seen from the above embodiments that the present invention provides a walnut germination promoter and a preparation method thereof, a method for promoting walnut seed germination, and an application thereof. The walnut germination promoter and the method for promoting walnut seed germination of the present invention can improve the germination rate and seedling rate of walnut seeds, and have the characteristics of high safety, strong stability, good effectiveness, etc.

[0076] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A walnut germination promoter, characterized in that The invention comprises the following components: gibberellin, naphthylacetic acid, triacontanol, indolebutyric acid and water; The mass volume ratio of the gibberellin to water is 30-80 mg:1 L; The mass volume ratio of naphthylacetic acid to water is 5-15 mg:1 L; The volume ratio of triacontanol to water is 1.5-5 mL:1 L; The mass volume ratio of the indolebutyric acid and water is 55-75 mg:1L.

2. Use of the walnut germination promoter according to claim 1 in promoting germination of walnut seeds.

3. The preparation method of the walnut germination promoter according to claim 1, characterized in that: The method comprises the following steps: mixing gibberellins, naphthylacetic acid, triacontanol, indolebutyric acid and water to obtain a walnut germination promoter.

4. A method for promoting germination of walnut seeds, characterized in that: The steps include: (1) soaking walnut seeds in warm water for 12 to 24 hours and drying to obtain pretreated seeds; (2) soaking the pretreated seeds in a walnut germination promoter for 24 to 48 hours, and drying them in the sun to obtain germinated seeds; (3) sowing the germinated seeds; The walnut germination promoter is the walnut germination promoter according to claim 1.

5. The method according to claim 4, characterized in that The temperature of the warm water in step (1) is 50-70°C.

6. The method according to claim 5, characterized in that The drying temperature in step (1) is 55-75° C. and the drying time is 3-6 hours.

7. The method according to claim 6, characterized in that The drying temperature in step (2) is 30-40° C., and the drying time is 2-5 days.

8. Use of the method according to any one of claims 4 to 7 in promoting germination of walnut seeds.