Automatic exosome separation and purification device

Abstract

The automatic exosome separation and purification device comprises a machine body shell, a sectional type separation and purification assembly, a movable ultrafiltration replacement mechanism, a cleaning device and a closed regulator, the machine body shell comprises a shell with a mounting opening formed in the front side and tempered glass mounted in the mounting opening, and a control cabinet is mounted on one side of the shell; the sectional type separation and purification assembly is vertically installed in the shell and comprises a hydraulic cylinder, a push rod, a piston, an outer barrel, a rotary inner barrel, a driver and a collecting barrel, the hydraulic cylinder is installed at the top of the shell, the power output end of the hydraulic cylinder is connected with the piston through the push rod, and the piston is movably inserted into the rotary inner barrel in a penetrating mode; a plurality of clamping teeth are machined and formed on the surface of the rotating inner cylinder. The separation and purification ultrafiltration device designed by the invention can realize the purpose of efficiently and automatically performing ultrafiltration purification and separation on liquid containing exosomes, and can perform automatic cleaning treatment on the used filter element so as to ensure the subsequent filtering effect.

Description

Technical Field

[0001] The present invention relates to the technical field of separation and purification of exosomes, and in particular to an automated exosome separation and purification device. Background Art

[0002] Exosomes are a type of small vesicles secreted by cells, which are released into the extracellular environment through the cell secretion pathway. These vesicles contain biological membranes and a variety of biological molecules, such as proteins, nucleic acids, lipids, etc., and have diverse functions and regulatory effects. Exosomes play an important role in biological processes such as intercellular communication, signal transmission, and waste disposal. They can regulate the functions of target cells by transporting bioinformatic molecules; participate in the removal and recycling of extracellular waste; and play a key role in the occurrence and development of diseases, such as their role in tumor growth, immune response, and inflammatory processes. The research field of exosomes is constantly expanding because people recognize the importance of exosomes under physiological and pathological conditions. In-depth research on exosomes helps to understand the mechanism of intercellular communication, the occurrence and development of diseases, and the development of potential biomedical applications. Exosome separation and purification is an important biological technology used to extract and purify exosomes from cell culture or biological fluids for subsequent research and application. The more common methods of exosome separation and purification are centrifugation and ultrafiltration. Compared with other methods, ultrafiltration is a relatively simple and rapid separation technology that can effectively remove cell debris and large molecular proteins, which helps to obtain relatively pure exosome samples. It has a high retention rate for exosomes and helps to avoid the loss of exosomes.

[0003] However, the existing devices for separating and purifying exosomes by ultrafiltration have the following problems during use: the core part of the ultrafiltration separation equipment is the ultrafiltration membrane, which separates exosomes from cell debris and macromolecular substances. However, in actual use, there are often more or less retained substances in the filter pores of the ultrafiltration membrane after each use, which will have different degrees of impact on the subsequent separation and purification. There is a lack of means for efficient treatment of the ultrafiltration membrane, and the ultrafiltration membrane separation and purification method is less efficient than the centrifugal separation method. Therefore, it is necessary to design corresponding technical solutions to solve the existing technical problems. Summary of the invention

[0004] The object of the present invention is to provide an automated exosome separation and purification device, which solves the technical problem that the core part of the ultrafiltration separation equipment is the ultrafiltration membrane, and the exosomes are separated from cell fragments and macromolecular substances through the ultrafiltration membrane. However, in the actual use process, there are often more or less retained substances in the filter pores of the ultrafiltration membrane after each use of the ultrafiltration membrane, which will have different degrees of influence on the subsequent separation and purification. There is a lack of means for efficient treatment of the ultrafiltration membrane, and the ultrafiltration membrane separation and purification method is less efficient than the centrifugal separation method.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an automated exosome separation and purification device, comprising a body shell, a segmented separation and purification component, a mobile ultrafiltration replacement mechanism, a cleaner and a closed regulator, the body shell comprising a shell having an installation opening on the front side and a tempered glass installed in the installation opening, a control cabinet is installed on one side of the shell, the segmented separation and purification component is vertically installed in the shell, the segmented separation and purification component comprises a hydraulic cylinder, a push rod, a piston, an outer cylinder, a rotating inner cylinder, a driver and a collecting cylinder, the hydraulic cylinder is installed on the top of the shell and the power output end is connected through the push rod The outer cylinder is sleeved on the outer side of the rotating outer cylinder and has a notch on one side. The top thread of the outer cylinder is arranged on the inner wall of the shell. The driver is installed in the notch. The collecting cylinder is located directly below the rotating inner cylinder. The closed regulator is installed on the outer cylinder and used in conjunction with the collecting cylinder. The mobile ultrafiltration replacement mechanism is horizontally arranged between the outer cylinder and the collecting cylinder. A cleaning chamber is provided on the left side of the shell. The cleaner is installed in the cleaning chamber. A drain pipe is externally connected to one side of the cleaning chamber.

[0006] As a preferred embodiment of the present invention, the driver includes a motor and a driving gear installed at a power output end of the motor, and the driving gear is meshed with a latching tooth.

[0007] As a preferred embodiment of the present invention, the closed regulator includes two groups of electric push rods symmetrically installed on the outer cylinder, and the power output ends of the two groups of electric push rods are connected to closed rings, and the thickness of the closed ring is greater than the distance between the outer cylinder and the collecting cylinder.

[0008] As a preferred embodiment of the present invention, two groups of bayonet holes are symmetrically provided at the bottom of the closed collar and the top of the collecting tube, and the upper and lower adjacent groups of bayonet holes are correspondingly distributed.

[0009] As a preferred embodiment of the present invention, the mobile ultrafiltration replacement mechanism includes an outer frame, a filter element, a connecting rod, a rack, a transmission gear and a servo motor. An inner opening is formed inside the outer frame. The filter element is divided into three groups and is evenly arranged in the inner opening. The two sides of the filter element are connected to the inner wall of the outer frame through two groups of connecting rods. The thickness of the filter element is equal to the spacing between the outer cylinder and the collecting cylinder. The rack is divided into two groups and is symmetrically installed on both sides of the outer frame. The transmission gear is meshed with the rack, and the power output end of the servo motor is connected to the transmission gear.

[0010] As a preferred embodiment of the present invention, the connecting rod is located between two upper and lower adjacent groups of bayonet holes, and the diameter of the connecting rod is equal to the diameter of the bayonet holes.

[0011] As a preferred embodiment of the present invention, the cleaning device includes a water tank, a water pump, a water supply pipe, a water guide pipe and a flushing tray. The water tank is installed on one side of the shell, the water pump is installed at the bottom of the water tank and the water inlet end is connected to the water tank through a pipe, the water outlet end of the water pump is connected to two groups of water guide pipes through two groups of water supply pipes, and the water guide pipe is connected to the flushing tray. The two groups of flushing trays are symmetrically arranged up and down.

[0012] As a preferred embodiment of the present invention, the flushing plate comprises a hollow plate body and a plurality of flushing pipes evenly mounted on the hollow plate body, and the outer ends of the flushing pipes are in a conical structure and face the filter element.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] 1. The present invention designs an ultrafiltration device specifically for separating and purifying exosomes, and the separation and purification ultrafiltration device includes a body shell, a segmented separation and purification component, a mobile ultrafiltration replacement mechanism, a cleaner and a closed regulator. When it is necessary to separate and purify the exosomes, the staff introduces the liquid containing the exosomes into the segmented separation and purification component, and places the filter element in the split separation and purification component through the mobile ultrafiltration replacement mechanism, and then closes the closed regulator, and squeezes, filters and separates the exosomes by means of pressurization. The purified and separated exosomes enter the bottom of the segmented separation and purification component, and the staff can take out the exosomes. After each operation, the filter element is replaced by the mobile ultrafiltration replacement mechanism and the used filter element is placed under the cleaner, and the filter element is bidirectionally rinsed and cleaned by the cleaner, thereby achieving the purpose of cleaning and reuse.

[0015] 2. The separation and purification ultrafiltration device designed in the present invention can achieve the purpose of efficient and automated ultrafiltration purification and separation of liquid containing exosomes, and can automatically clean the filter element after use to ensure the effect of subsequent filtration. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is the overall structure diagram of the present invention;

[0017] Figure 2 This is a structural diagram of the segmented separation and purification component of the present invention;

[0018] Figure 3 This is a structural diagram of the closed state of the closed regulator described in the present invention.

[0019] Figure 4 This is a structural diagram of the mobile ultrafiltration replacement mechanism of the present invention;

[0020] Figure 5 This is a top view of the structure of the mobile ultrafiltration replacement mechanism of the present invention.

[0021] In the figure: 1. shell; 2. tempered glass; 3. control cabinet; 4. hydraulic cylinder; 5. push rod; 6. piston; 7. outer cylinder; 8. rotating inner cylinder; 9. driver; 10. collecting cylinder; 11. latch; 12. notch; 13. cleaning chamber; 14. drain pipe; 15. motor; 16. driving gear; 17. electric push rod; 18. closing ring; 19. latch; 20. outer frame; 21. filter element; 22. connecting rod; 23. rack; 24. transmission gear; 25. servo motor; 26. embedded port; 27. water tank; 28. water pump; 29. ​​water pipe; 30. water guide pipe; 31. flushing tray; 32. hollow tray; 33. flushing pipe. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] See also Figure 1-5The present invention provides a technical solution: an automated exosome separation and purification device, comprising a body shell, a segmented separation and purification component, a mobile ultrafiltration replacement mechanism, a cleaner and a closed regulator, the body shell comprising a shell 1 with an installation opening on the front side and a tempered glass 2 installed in the installation opening, a control cabinet 3 is installed on one side of the shell 1, the segmented separation and purification component is vertically installed in the shell 1, the segmented separation and purification component comprises a hydraulic cylinder 4, a push rod 5, a piston 6, an outer cylinder 7, a rotating inner cylinder 8, a driver 9 and a collecting cylinder 10, the hydraulic cylinder 4 is installed on the top of the shell 1 and the power output end is connected to the piston 6 through the push rod 5 The piston 6 is movably inserted into the rotating inner cylinder 8, and the surface of the rotating inner cylinder 8 is processed to form a plurality of groups of latch teeth 11. The outer cylinder 7 is embedded in the outer side of the rotating outer cylinder 7 and a notch 12 is opened on one side. The top thread of the outer cylinder 7 is set on the inner wall of the shell 1, and the driver 9 is installed in the notch 12. The collecting cylinder 10 is located directly below the rotating inner cylinder 8. The closed regulator is installed on the outer cylinder 7 and used in conjunction with the collecting cylinder 10. The mobile ultrafiltration replacement mechanism is horizontally arranged between the outer cylinder 7 and the collecting cylinder 10. A cleaning chamber 13 is opened on the left side of the shell 1, and the washer is installed in the cleaning chamber 13. A drain pipe 14 is externally connected to one side of the cleaning chamber 13.

[0024] Further improvement, such as Figure 2 As shown, the driver 9 includes a motor 15 and a driving gear 16 installed at the power output end of the motor 15. The driving gear 16 is meshed with the latch 11. The motor 15 drives the driving gear 16 to rotate. During the rotation, the driving gear 16 drives the rotating inner cylinder 8 to rotate and adjust, so as to prevent impurities inside the liquid from being deposited at the bottom of the rotating inner cylinder 8.

[0025] Further improvement, such as Figure 2 and 3 As shown, the closed regulator includes two sets of electric push rods 17 symmetrically installed on the outer cylinder 7, and the power output ends of the two sets of electric push rods 17 are connected with closed rings 18. The thickness of the closed ring 18 is greater than the distance between the outer cylinder 7 and the collecting cylinder 10. The electric push rods 17 can drive the chalk ring 18 to adjust longitudinally, so that the filter element 21 can be closed.

[0026] Further improvement, such as Figure 2 As shown, two groups of bayonet holes 19 are symmetrically provided at the bottom of the closing ring 18 and the top of the collecting tube 10 , and the upper and lower adjacent groups of bayonet holes 19 are correspondingly distributed, so that the filter element 21 can be sealed by the closing ring 18 .

[0027] Further improvement, such as Figure 4As shown, the mobile ultrafiltration replacement mechanism includes an outer frame 20, a filter element 21, a connecting rod 22, a rack 23, a transmission gear 24 and a servo motor 25. An inner opening 26 is formed inside the outer frame 20. The filter element 21 is divided into three groups and is evenly arranged in the inner opening 26. Both sides of the filter element 21 are connected to the inner wall of the outer frame 20 through two groups of connecting rods 22. The thickness of the filter element 21 is equal to the spacing between the outer cylinder 7 and the collecting cylinder 10. The rack 23 is divided into two groups and is symmetrically installed on both sides of the outer frame 20. The transmission gear 24 is meshed with the rack 23. The power output end of the servo motor 25 is connected to the transmission gear 24. When it is necessary to clean the used filter element 21, the transmission gear 24 is driven to rotate by the servo motor 25. During the rotation, the transmission gear 24 moves the used filter element 21 to the left and enters the cleaning chamber 13, and the used filter element 21 is cleaned by the cleaner.

[0028] Further improvement, such as Figure 3 As shown, the connecting rod 22 is located between two adjacent groups of bayonet holes 19 , and the diameter of the connecting rod 22 is equal to the diameter of the bayonet holes 19 , so as to achieve the purpose of sealing and fixing the filter element 21 .

[0029] Further improvement, such as Figure 1 and 4 As shown, the cleaning device includes a water tank 27, a water pump 28, a water pipe 29, a water conduit 30 and a flushing tray 31. The water tank 27 is installed on one side of the housing 1. The water pump 28 is installed at the bottom of the water tank 27 and the water inlet end is connected to the water tank 27 through a pipeline. The water outlet end of the water pump 28 is respectively connected to two groups of water conduits 30 through two groups of water pipes 29. The water conduits 30 are connected to the flushing tray 31. The two groups of flushing trays 31 are symmetrically arranged up and down. The water in the water tank 27 is pumped out by the water pump 28 and transported to the water conduits 30 through the water pipes 29. The water conduits 30 guide the water flow to the flushing tray 31, and the filter element 21 is flushed through the flushing tray 31. In addition, the two flushing trays 31 work alternately to achieve the purpose of efficiently flushing and cleaning the filter holes inside the filter element 21.

[0030] Specifically, the flushing plate 31 includes a hollow plate body 32 and a plurality of flushing tubes 33 evenly mounted on the hollow plate body 32 . The outer ends of the flushing tubes 33 are conical in structure and face the filter element 21 . The filter element is continuously flushed and cleaned through the flushing tubes 33 .

[0031] During use: When ultrafiltration separation of exosomes is required, the staff introduces the solution containing exosomes into the rotating inner cylinder 8, and drives the driving gear 16 to rotate through the motor 15. The driving gear 16 drives the rotating inner cylinder 8 to rotate and adjust during the rotation to prevent impurities in the liquid from being deposited at the bottom of the rotating inner cylinder 8. At the same time, the hydraulic cylinder 4 pushes the push rod 5 and the piston 6 downward and applies pressure to the solution to improve the filtration efficiency. The internal filter element 21 is used to separate and purify the exosomes. When the filter element 21 needs to be cleaned after use, The transmission gear 24 is driven to rotate by the servo motor 25. During the rotation, the transmission gear 24 moves the used filter element 21 to the left and enters the cleaning chamber 13. The used filter element 21 is cleaned by the cleaner. The water in the water tank 27 is pumped out by the water pump 28 and transported to the water pipe 30 through the water pipe 29. The water pipe 30 guides the water flow to the flushing tray 31, and the filter element 21 is flushed by the flushing tray 31. In addition, the two flushing trays 31 work alternately to achieve the purpose of efficiently flushing and cleaning the filter holes inside the filter element 21.

[0032] In the description of the present invention, it is necessary to understand that the terms "coaxial", "bottom", "one end", "top", "middle", "the other end", "upper", "one side", "top", "inside", "front", "center", "both ends" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0033] In addition, the terms "first", "second", "third" and "fourth" are used for descriptive purposes only and are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, the features defined as "first", "second", "third" and "fourth" may explicitly or implicitly include at least one of such features.

[0034] In the present invention, unless otherwise clearly stipulated and limited, the terms such as "installation", "setting", "connection", "fixation" and "screw-on" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be the internal connection of two elements or the interaction relationship between two elements. Unless otherwise clearly defined, ordinary technicians in this field can understand the specific meanings of the above terms in the present invention according to the specific circumstances.

[0035] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An automated exosome separation and purification device, characterized in that: The invention comprises a body shell, a segmented separation and purification component, a mobile ultrafiltration replacement mechanism, a cleaning device and a closed regulator. The body shell comprises a shell (1) with an installation opening on the front side and a tempered glass (2) installed in the installation opening. A control cabinet (3) is installed on one side of the shell (1). The segmented separation and purification component is vertically installed in the shell (1). The segmented separation and purification component comprises a hydraulic cylinder (4), a push rod (5), a piston (6), an outer cylinder (7), a rotating inner cylinder (8), a driver (9) and a collecting cylinder (10). The hydraulic cylinder (4) is installed on the top of the shell (1) and the power output end is connected to the piston (6) through the push rod (5). The piston (6) is movably inserted into the rotating inner cylinder (8). The surface of the rotating inner cylinder (8) is machined to form a plurality of groups of latch teeth (11); the outer cylinder (7) is sleeved on the outer side of the rotating outer cylinder (7) and is provided with a notch (12) on one side; the top thread of the outer cylinder (7) is arranged on the inner wall of the shell (1); the driver (9) is installed in the notch (12); the collecting cylinder (10) is located directly below the rotating inner cylinder (8); the closed regulator is installed on the outer cylinder (7) and is used in conjunction with the collecting cylinder (10); the mobile ultrafiltration replacement mechanism is horizontally arranged between the outer cylinder (7) and the collecting cylinder (10); a cleaning chamber (13) is provided on the left side of the shell (1); the cleaner is installed in the cleaning chamber (13); and a drainage pipe (14) is externally connected to one side of the cleaning chamber (13).

2. An automated exosome separation and purification device according to claim 1, characterized in that: The driver (9) comprises a motor (15) and a driving gear (16) installed at a power output end of the motor (15), and the driving gear (16) is meshed with the latching gear (11).

3. An automated exosome separation and purification device according to claim 1, characterized in that: The closed regulator comprises two groups of electric push rods (17) symmetrically mounted on the outer cylinder (7), the power output ends of the two groups of electric push rods (17) being connected with a closed collar (18), the thickness of the closed collar (18) being greater than the distance between the outer cylinder (7) and the collecting cylinder (10).

4. The automated exosome separation and purification device according to claim 3, characterized in that: The bottom of the sealing ring (18) and the top of the collecting tube (10) are both symmetrically provided with two groups of bayonet holes (19), and the upper and lower adjacent groups of bayonet holes (19) are correspondingly distributed.

5. An automated exosome separation and purification device according to claim 4, characterized in that: The mobile ultrafiltration replacement mechanism comprises an outer frame (20), a filter element (21), a connecting rod (22), a rack (23), a transmission gear (24) and a servo motor (25); an inner opening (26) is formed inside the outer frame (20); the filter element (21) is divided into three groups and is evenly arranged in the inner opening (26); both sides of the filter element (21) are connected to the inner wall of the outer frame (20) through two groups of connecting rods (22); the thickness of the filter element (21) is equal to the spacing between the outer cylinder (7) and the collecting cylinder (10); the rack (23) is divided into two groups and is symmetrically installed on both sides of the outer frame (20); the transmission gear (24) is meshed with the rack (23); and the power output end of the servo motor (25) is connected to the transmission gear (24).

6. An automated exosome separation and purification device according to claim 5, characterized in that: The connecting rod (22) is located between two adjacent groups of bayonet holes (19) at the upper and lower ends, and the diameter of the connecting rod (22) is equal to the diameter of the bayonet holes (19).

7. The automated exosome separation and purification device according to claim 1, characterized in that: The cleaning device comprises a water tank (27), a water pump (28), a water delivery pipe (29), a water guide pipe (30) and a flushing tray (31); the water tank (27) is installed on one side of the housing (1); the water pump (28) is installed at the bottom of the water tank (27) and the water inlet end is connected to the water tank (27) through a pipeline; the water outlet end of the water pump (28) is respectively connected to two groups of water guide pipes (30) through two groups of water delivery pipes (29); the water guide pipe (30) is connected to the flushing tray (31); and the two groups of flushing trays (31) are symmetrically arranged up and down.

8. An automated exosome separation and purification device according to claim 7, characterized in that: The flushing disc (31) comprises a hollow disc body (32) and a plurality of groups of flushing pipes (33) evenly mounted on the hollow disc body (32); the outer ends of the flushing pipes (33) are in a conical structure and face the filter element (21).

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