Automatic separation and cultivation equipment for microorganisms

Abstract

The invention relates to the technical field of microorganism separation and cultivation, in particular to microorganism automatic separation and cultivation equipment which comprises a base and a centrifugal pan rotationally arranged on the base, a top cover matched with the centrifugal pan in a closed mode is detachably arranged at the upper end of the centrifugal pan, and a circulating pump is arranged on the top cover. The second driving assembly drives the precipitation bottom groove and the storage cylinder to synchronously overturn in the positive and negative directions, so that the center lines of the precipitation bottom groove and the storage cylinder are kept in the horizontal or vertical state, the centrifugal disc is driven to rotate in cooperation with the first driving assembly, centrifugal operation is completed, and formed precipitation microorganisms are placed in the precipitation bottom groove matched with the large-diameter end opening of the storage cylinder. The supernatant is placed in the storage barrel, so that the precipitated microorganisms are prevented from flowing back to the supernatant again, the separation effect is guaranteed, the precipitated microorganisms are automatically discharged into the culture medium through the material taking assembly to be cultivated, cultivation conditions suitable for different types of microorganisms are provided, and the application range is wide.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganism separation and cultivation, and in particular to an automatic microorganism separation and cultivation device. Background Art

[0002] The cultivation step in drug microbial detection and analysis is an important link to ensure that the test results are accurate and reliable. Usually, the solution containing drug microorganisms is directly filtered or centrifuged to separate the microorganisms, and then the separated microorganisms are placed on a culture medium suitable for microbial growth for cultivation. The existing technology uses a more efficient centrifugal sedimentation method with a corresponding separation device to achieve rapid separation of microorganisms.

[0003] For example, the Chinese patent application number CN202410330384.7 discloses a microbial separation device for drug microbial testing. The separation device body includes a centrifugal mechanism, a separation component and a dialysis component. By installing multiple dialysis components at the edge of the centrifugal mechanism in an inclined state, the initial separation process of the microbial precipitate and the supernatant can be completed during the centrifugation process. With the subsequent lifting and lowering motion control of the separation component, the purpose of rapid separation and efficient repeated separation can be achieved, making the microbial separation more thorough. However, in this separation device, after the microbial base liquid is centrifuged inside the centrifugal channel, the precipitated microorganisms accumulated on the surface of the dialysis component will flow back into the supernatant again when the centrifugation is stopped, and the separation effect is not ideal. After the microbial precipitate enters the collection bin through the annular docking port, the docking bin can be opened to take out the microbial precipitate. However, for anaerobic microorganisms, contact with the air environment during the separation and cultivation process will cause the microorganism to die or its growth is inhibited, resulting in a limited scope of application of the separation device. Summary of the invention

[0004] In view of this, the purpose of the present invention is to propose an automated microbial separation and cultivation device to solve the technical problems in the prior art that when the centrifugation is stopped, the precipitated microorganisms will flow back into the supernatant again, the separation effect is not ideal, and the scope of application is limited.

[0005] Based on the above purpose, the present invention provides an automated microbial separation and cultivation device, comprising a base and a centrifugal disc rotatably arranged on the base, the upper end of the centrifugal disc is detachably provided with a top cover that is sealed and matched with the centrifugal disc, the top cover is provided with a circulation pump, the circulation pump is connected to the closed inner cavity formed by the centrifugal disc and the top cover through a circulation pipeline, a plurality of culture media are circumferentially installed on the centrifugal disc, and the automated separation and cultivation device also includes:

[0006] A storage cylinder, which is a conical cavity structure with openings at both ends. There are a plurality of storage cylinders, and the plurality of storage cylinders can be turned over and arranged in a closed inner cavity;

[0007] A sedimentation bottom tank, on which the large-diameter port of the storage cylinder is mounted;

[0008] A valve cover installed at the small-diameter port of the storage cylinder;

[0009] A connecting pipe, one end of which is mounted on the valve cover, and the other end of which extends to the hemispherical bottom of the sedimentation tank, the hemispherical bottom of the sedimentation tank is provided with at least two discharge openings, and an opening and closing member is provided on the inner wall of the hemispherical bottom of the sedimentation tank at the discharge openings;

[0010] A material taking head matched with the discharge opening, the material taking head is controlled to open and close by a solenoid valve and is installed on the centrifugal disk through a material taking component, and is connected with the culture medium through the material taking component;

[0011] A driving component 1, wherein the driving component 1 is used to drive the centrifugal disk to rotate so as to centrifugally separate the microbial base liquid in the plurality of storage cylinders;

[0012] A driving component 2, wherein the driving component 2 is used to drive the storage cylinder to flip so that its center line remains horizontal or vertical. When its center line is in a horizontal state, the material taking head is synchronously driven to extend into the sedimentation bottom tank to absorb the precipitated microorganisms after centrifugal separation, and automatically discharge them into the culture medium for cultivation through the material taking component. When its center line is in a vertical state, the connecting pipe is connected to the circulation pipeline.

[0013] Furthermore, the driving component 1 includes:

[0014] A driving motor, wherein the driving motor is mounted on the base through a mounting frame;

[0015] A driving wheel, the driving wheel is installed on the output end of the driving motor;

[0016] The driven wheel is rotatably mounted on the base via a rotating shaft, the driven wheel is meshed with the driving wheel, and the upper end of the rotating shaft is coaxially mounted on the lower end surface of the centrifugal disc.

[0017] Furthermore, the second driving component includes:

[0018] A cylinder, wherein the cylinder is mounted on the upper end surface of the centrifugal disk;

[0019] A lifting frame, the lifting frame is installed on the output end of the cylinder, and a plurality of bracket plates extend from the side end of the lifting frame;

[0020] A fixing seat, the fixing seat is installed inside the centrifugal disc;

[0021] A support rod, the lower end of which is mounted on the bracket plate, and the support rod is also slidably mounted on the fixing seat;

[0022] A slider, the slider being hinged to the upper end of the support rod;

[0023] A support platform, the support platform is fixedly mounted on the fixing seat;

[0024] A guide rod, one end of which is hinged on the support platform, the sedimentation trough is fixedly connected to the end of the guide rod, the other end of the guide rod is clamped on the valve cover, and the slider is also slidably arranged on the guide rod.

[0025] Furthermore, the opening and closing member includes a reed and a closing piece, wherein the reed is fixedly mounted on the inner wall of the hemispherical bottom of the sedimentation trough, and the closing piece is mounted on the end of the reed, and the closing piece covers the discharge opening.

[0026] Furthermore, the material taking component comprises:

[0027] A shunt seat, the shunt seat is fixedly mounted on the centrifugal disk, a centrifugal channel is provided inside the shunt seat, a docking port is provided at a side end of the culture medium, and the docking port is communicated with the centrifugal channel;

[0028] A flip arm, the material taking head is installed on the flip arm, the lower end of the flip arm is hinged on the diverter seat, the material taking head is connected with the centrifugal channel through the cavity structure of the flip arm, and an inclined slot is provided on the flip arm;

[0029] A slide bar, the slide bar is slidably disposed on the diverter seat, and the lower end of the slide bar is connected to the lifting frame;

[0030] A sliding head, wherein the middle portion of the sliding head is mounted on the top end of the sliding rod, and both ends of the sliding head are slidably disposed in the inclined groove;

[0031] A pressure pump is installed at the upper end of the diverter seat, and a working end of the pressure pump is connected to the centrifugal channel.

[0032] Furthermore, there are at least two flip arms, and after the ends of the two flip arms are flipped close to each other, the material taking head extends into the sedimentation bottom tank through the discharge opening, and the opening and closing piece is in an open state.

[0033] Furthermore, both ends of the sedimentation bottom tank are provided with elastic clamping sheets, and the clamping ends of the elastic clamping sheets match with the outer end surface of the storage tube.

[0034] Furthermore, a stabilizing portion for positioning the valve cover is also installed on the fixing seat, and the stabilizing portion includes:

[0035] A guide rod, the guide rod being mounted on the fixing seat;

[0036] A mounting block, the mounting block being slidably disposed on the guide rod;

[0037] A spring, wherein the spring is sleeved on the guide rod, one end of the spring abuts against the mounting block, and the other end of the spring abuts against the fixing seat;

[0038] A clamping plate is mounted on the upper end of the mounting block, and the edge end of the valve cover is clamped on the clamping plate.

[0039] Furthermore, a plurality of positioning blocks are circumferentially mounted on the inner wall of the top cover, and a plurality of positioning blocks are provided with positioning grooves, and a plurality of positioning grooves are matched with the outer side wall of the storage tube.

[0040] Furthermore, a side end of the valve cover is provided with an avoidance groove, and a groove depth of the avoidance groove is not less than a protruding length of the positioning groove.

[0041] Beneficial effects of the present invention: When the microbial automated separation and cultivation equipment of the present invention is used, the driving component 2 drives the sedimentation bottom tank and the storage cylinder to flip synchronously in the forward and reverse directions, so that the center line thereof remains in a horizontal or vertical state, and the driving component 1 drives the centrifugal disk to rotate to complete the centrifugal operation, and the formed precipitated microorganisms are placed in the sedimentation bottom tank matched with the large-diameter port of the storage cylinder, and the supernatant is placed in the storage cylinder, thereby preventing the precipitated microorganisms from flowing back into the supernatant again, thereby ensuring the separation effect, and the precipitated microorganisms are automatically discharged into the culture medium for cultivation through the material taking component, thereby providing cultivation conditions adapted to different types of microorganisms, and having a wide range of applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only for the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0043] Figure 1 It is a schematic diagram of the internal structure of the present invention;

[0044] Figure 2 It is a schematic diagram of the external structure of the present invention;

[0045] Figure 3It is a schematic diagram of the assembly of the storage cylinder and some structures in the horizontal state in the present invention;

[0046] Figure 4 It is a schematic diagram of the assembly of the internal structure of the second driving component in the present invention;

[0047] Figure 5 It is a schematic diagram of the structure of the sedimentation bottom tank in the present invention;

[0048] Figure 6 It is a schematic diagram of the assembly of the material taking component in the present invention;

[0049] Figure 7 It is a schematic diagram of the internal structure of the material taking component in the present invention;

[0050] Figure 8 It is a schematic diagram of the state of the driving component 1 and the top cover in the present invention.

[0051] The markings in the figure are:

[0052] 1. Base; 2. Centrifugal disc; 3. Top cover; 4. Circulation pump; 5. Circulation pipeline; 6. Culture medium;

[0053] 7. Storage cylinder; 8. Sedimentation bottom tank; 9. Valve cover; 10. Connecting pipe; 11. Discharge opening; 12. Material taking head; 13. Driving motor; 14. Mounting frame; 15. Driving wheel; 16. Driven wheel; 17. Cylinder; 18. Lifting frame; 19. Fixed seat; 20. Support rod; 21. Sliding block; 22. Support platform; 23. Guide rod; 24. Spring; 25. Closing plate; 26. Diverter seat; 27. Centrifugal channel; 28. Docking port; 29. ​​Flip arm; 30. Inclined groove; 31. Sliding rod; 32. Sliding head; 33. Pressure pump; 34. Elastic clamping plate; 35. Guide rod; 36. Mounting block; 37. Spring; 38. Card plate; 39. Positioning block; 40. Positioning groove; 41. Avoidance groove. DETAILED DESCRIPTION

[0054] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments.

[0055] It should be noted that, unless otherwise defined, the technical terms or scientific terms used in the present invention should be understood by people with ordinary skills in the field to which the present invention belongs. The "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. "Include" or "comprise" and similar words mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0056] The first aspect of the present invention provides an automated microbial separation and cultivation device, such as Figure 1-8 As shown, it includes a base 1 and a centrifugal disc 2 rotatably arranged on the base 1, a top cover 3 which is detachably arranged on the upper end of the centrifugal disc 2 and is sealed with the centrifugal disc 2, a circulating pump 4 is arranged on the top cover 3, and the circulating pump 4 is connected to the closed inner cavity formed by the centrifugal disc 2 and the top cover 3 through a circulation pipe 5, and a plurality of culture media 6 are circumferentially installed on the centrifugal disc 2. The automated separation and cultivation equipment also includes:

[0057] The storage cylinder 7 is a conical cavity structure with openings at both ends. There are a plurality of storage cylinders 7, and the plurality of storage cylinders 7 can be turned over and disposed in the closed inner cavity;

[0058] The sedimentation bottom tank 8, the large diameter port of the storage tube 7 is installed on the sedimentation bottom tank 8;

[0059] A valve cover 9, the valve cover 9 is installed at the small-diameter port of the storage tube 7;

[0060] A connecting pipe 10, one end of which is mounted on the valve cover 9, and the other end of which extends to the hemispherical bottom of the sedimentation tank 8, the hemispherical bottom of the sedimentation tank 8 is provided with at least two discharge openings 11, and an opening and closing member is provided on the inner wall of the hemispherical bottom of the sedimentation tank 8 at the discharge openings 11;

[0061] A material taking head 12 matched with the discharge opening 11, the material taking head 12 is controlled to open and close by a solenoid valve and is installed on the centrifugal disk 2 through a material taking component, and is connected to the culture medium 6 through the material taking component;

[0062] Driving assembly 1, driving assembly 1 is used to drive the centrifugal disc 2 to rotate, so as to centrifugally separate the microbial base liquid in the plurality of storage cylinders 7;

[0063] Driving component 2, driving component 2 is used to drive the storage cylinder 7 to flip so that its center line remains horizontal or vertical. When its center line is in a horizontal state, it synchronously drives the material taking head 12 to extend into the sedimentation bottom tank 8 to absorb the precipitated microorganisms after centrifugal separation, and automatically discharges them into the culture medium 6 for cultivation through the material taking component. When its center line is in a vertical state, the connecting pipe 10 is connected to the circulation pipe 5.

[0064] In this embodiment, the driving component 1 includes:

[0065] A driving motor 13, the driving motor 13 is mounted on the base 1 through a mounting bracket 14;

[0066] A driving wheel 15, which is mounted on the output end of the driving motor 13;

[0067] The driven wheel 16 is rotatably mounted on the base 1 via a rotating shaft. The driven wheel 16 is meshed with the driving wheel 15 . The upper end of the rotating shaft is coaxially mounted on the lower end surface of the centrifugal disc 2 .

[0068] In this embodiment, the driving component 2 includes:

[0069] Cylinder 17, cylinder 17 is installed on the upper end surface of the centrifugal disc 2;

[0070] A lifting frame 18, the lifting frame 18 is installed on the output end of the cylinder 17, and a plurality of bracket plates extend from the side end of the lifting frame 18;

[0071] A fixing seat 19, the fixing seat 19 is installed inside the centrifugal disc 2;

[0072] A support rod 20, the lower end of which is mounted on the bracket plate, and the support rod 20 is also slidably mounted on the fixing seat 19;

[0073] A slider 21, the slider 21 is hinged to the upper end of the support rod 20;

[0074] A support platform 22, the support platform 22 is fixedly mounted on the fixing seat 19;

[0075] The guide rod 23 has one end hinged on the support platform 22 , the sedimentation trough 8 is fixedly connected to the end of the guide rod 23 , and the other end of the guide rod 23 is clamped on the valve cover 9 . The slider 21 is also slidably arranged on the guide rod 23 .

[0076] In this embodiment, the material taking component includes:

[0077] A flow divider 26 is fixedly mounted on the centrifugal disk 2. A centrifugal channel 27 is provided inside the flow divider 26. A docking port 28 is provided at a side end of the culture medium 6. The docking port 28 is connected to the centrifugal channel 27.

[0078] The flip arm 29, the material taking head 12 is installed on the flip arm 29, the lower end of the flip arm 29 is hinged on the diverter seat 26, the material taking head 12 is connected with the centrifugal channel 27 through the cavity structure of the flip arm 29, and the flip arm 29 is provided with an inclined slot 30;

[0079] A slide bar 31, the slide bar 31 is slidably disposed on the diverter seat 26, and the lower end of the slide bar 31 is connected to the lifting frame 18;

[0080] A sliding head 32, the middle portion of the sliding head 32 is mounted on the top end of the sliding rod 31, and both ends of the sliding head 32 are slidably disposed in the inclined groove 30;

[0081] The pressure pump 33 is installed at the upper end of the diverter seat 26 , and the working end of the pressure pump 33 is connected to the centrifugal channel 27 .

[0082] In this embodiment, when in use, the storage cylinder 7 is driven by the second driving component to flip upward so that its center line is in a vertical state, that is, the output end of the cylinder 17 drives the lifting frame 18 to lift upward, and the support rod 20 drives the slider 21 to slide on the guide rod 23, thereby pushing the sedimentation bottom tank 8 to flip around the hinge point with the support platform 22, so that the storage cylinder 7 on the sedimentation bottom tank 8 is flipped synchronously, and then the connecting pipe 10 is connected with the circulation pipeline 5, and the microbial base liquid to be separated is added to each storage cylinder 7 through the circulation pump 4, the circulation pipeline 5 and the connecting pipe 10. Under the sealing effect of the opening and closing member, the microbial base liquid will not flow out of the sedimentation bottom tank 8;

[0083] After an appropriate amount of microbial base liquid enters, the connecting pipe 10 is closed by the valve cover 9, and then the storage cylinder 7 is driven downward to flip by the driving component 2. After the above operation is reversed, the center line of the storage cylinder 7 is in a horizontal state. During this process, the lifting frame 18 will also drive the sliding head 32 to slide in the inclined groove 30 through the sliding rod 31 to pull the flip arm 29 to flip, thereby driving the material taking head 12 to extend into the discharge opening 11;

[0084] Then, the driving assembly 1 is started, and the driving motor 13 therein drives the driving wheel 15 to rotate, and the centrifugal disc 2 is driven to rotate centrifugally through the driven wheel 16 and the rotating shaft meshed with the driving motor 13. After the centrifugal operation is completed, the driving motor 13 is turned off, so that the centrifugal disc 2 gradually stops rotating, and the formed precipitated microorganisms are placed in the precipitation bottom tank 8 matched with the large-diameter port of the storage cylinder 7, and the supernatant is placed in the storage cylinder 7;

[0085] When detecting and analyzing anaerobic microorganisms, the solenoid valve and the pressure pump 33 on the material taking head 12 are opened at the same time. The pressure pump 33 provides a negative pressure environment to quickly extract the air in the centrifugal channel 27 and the culture medium 6 to form the anaerobic environment conditions required by the microorganisms. The precipitated microorganisms in the sedimentation bottom tank 8 are absorbed by the material taking head 12 and enter the culture medium 6 through the cavity structure of the flip arm 29, the centrifugal channel 27 and the docking port 28. When detecting and analyzing aerobic microorganisms or insensitive microorganisms, there is no need to open the pressure pump 33. The precipitated microorganisms will flow into the culture medium 6 along the flow path. The culture medium 6 contains a coagulant such as agar, gelatin or carrageenan. The microorganisms grow on the inner surface or inside the culture medium 6 and are eventually cultivated into colonies visible to the naked eye for microbial detection and analysis, identification, and live bacteria counting.

[0086] In this embodiment, if Figure 4 As shown, the opening and closing member includes a reed 24 and a closing piece 25. The reed 24 is fixedly mounted on the inner wall of the hemispherical bottom of the sedimentation trough 8, and the closing piece 25 is mounted on the end of the reed 24. The closing piece 25 covers the discharge opening 11. When the material taking head 12 does not extend into the discharge opening 11, the closing piece 25 can completely block the discharge opening 11 under the elastic force of the reed 24 itself to avoid leakage.

[0087] In this embodiment, if Figure 3 , Figure 6 As shown, there are at least two flip arms 29. After the ends of the two flip arms 29 are flipped close to each other, the material taking head 12 extends into the sedimentation bottom tank 8 through the discharge opening 11, and the opening and closing part is in an open state, so that the material taking head 12 can completely absorb the precipitated microorganisms in the sedimentation bottom tank 8, avoiding the situation where the microorganisms on one side are insufficiently absorbed and the microorganisms on the other side accumulate.

[0088] In this embodiment, if Figure 3 , Figure 4 , Figure 5 As shown, both ends of the sedimentation bottom tank 8 are provided with elastic clamping pieces 34, and the clamping ends of the elastic clamping pieces 34 cooperate with the outer end surface of the storage cylinder 7 to maintain the stability of the storage cylinder 7 and the sedimentation bottom tank 8 during the centrifugation process.

[0089] In this embodiment, if Figure 1 , Figure 3 As shown, a stabilizing portion for positioning the valve cover 9 is also mounted on the fixing seat 19, and the stabilizing portion includes:

[0090] A guide rod 35, the guide rod 35 is mounted on the fixing seat 19;

[0091] A mounting block 36, the mounting block 36 is slidably disposed on the guide rod 35;

[0092] Spring 37, spring 37 is sleeved on the guide rod 35, one end of the spring 37 abuts against the mounting block 36, and the other end of the spring 37 abuts against the fixing seat 19;

[0093] The card plate 38 is installed on the upper end of the mounting block 36, and the edge end of the valve cover 9 is clamped on the card plate 38. During the centrifugation process, the valve cover 9 can remain stable on the card plate 38 to avoid vibration of the storage cylinder 7 during the rotating centrifugation process, thereby ensuring that the precipitated microorganisms and the supernatant are in a stable state in the storage cylinder 7.

[0094] In this embodiment, if Figure 1 , Figure 8 As shown, a plurality of positioning blocks 39 are annularly installed on the inner wall of the top cover 3, and a plurality of positioning blocks 39 are provided with positioning grooves 40, and a plurality of positioning grooves 40 are matched with the outer wall of the storage cylinder 7. A side end of the valve cover 9 is provided with an avoidance groove 41, and the groove depth of the avoidance groove 41 is not less than the protruding length of the positioning groove 40. After the separation and cultivation operation is completed, a positive air pressure is provided to the microbial flow path by the pressure pump 33 to automatically clean the flow path, and then, under the positioning of the positioning groove 40, the storage cylinder 7 is in a vertical state, the top cover 3 is opened, and the residual solution inside is sucked by the connecting pipe 10, so as to realize the rapid cleaning of the entire separation and cultivation equipment.

[0095] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of the present invention (including the claims) is limited to these examples. Under the concept of the present invention, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

[0096] The present invention is intended to cover all such substitutions, modifications and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An automated microbial separation and cultivation device, comprising a base (1) and a centrifugal disc (2) rotatably mounted on the base (1), wherein a top cover (3) which is detachably mounted on the upper end of the centrifugal disc (2) and which is sealed and matched with the centrifugal disc (2), wherein a circulating pump (4) is mounted on the top cover (3), and the circulating pump (4) is connected to a closed inner cavity formed by the centrifugal disc (2) and the top cover (3) through a circulation pipe (5), wherein: A plurality of culture media (6) are annularly mounted on the centrifugal disk (2), and the automated separation and cultivation equipment further comprises: A storage cylinder (7), wherein the storage cylinder (7) is a conical cavity structure with openings at both ends, and there are a plurality of storage cylinders (7), and the plurality of storage cylinders (7) can be turned over and arranged in the closed inner cavity; A sedimentation bottom tank (8), on which the large-diameter port of the storage cylinder (7) is mounted; A valve cover (9), the valve cover (9) being installed at the small-diameter port of the storage cylinder (7); a connecting pipe (10), one end of the connecting pipe (10) being mounted on the valve cover (9), the other end of the connecting pipe (10) extending to the hemispherical bottom of the sedimentation trough (8), the hemispherical bottom of the sedimentation trough (8) being provided with at least two discharge openings (11), and an opening and closing member being provided on the inner wall of the hemispherical bottom of the sedimentation trough (8) at the discharge openings (11); a material taking head (12) matched with the discharge opening (11), the material taking head (12) is controlled to open and close by a solenoid valve and is installed on the centrifugal disk (2) through a material taking component, and is connected to the culture medium (6) through the material taking component; A driving component 1, the driving component 1 is used to drive the centrifugal disc (2) to rotate so as to centrifugally separate the microbial base liquid in the plurality of storage cylinders (7); A driving component 2, wherein the driving component 2 is used to drive the storage cylinder (7) to flip so that its center line remains in a horizontal or vertical state. When its center line is in a horizontal state, the material taking head (12) is synchronously driven to extend into the sedimentation bottom tank (8) to absorb the precipitated microorganisms after centrifugal separation, and automatically discharge them into the culture medium (6) for cultivation through the material taking component. When its center line is in a vertical state, the connecting pipe (10) is connected to the circulation pipe (5).

2. The microbial automated separation and cultivation equipment according to claim 1, characterized in that: The driving component 1 comprises: A driving motor (13), wherein the driving motor (13) is mounted on the base (1) via a mounting frame (14); A driving wheel (15), wherein the driving wheel (15) is mounted on the output end of the driving motor (13); A driven wheel (16) is rotatably mounted on the base (1) via a rotating shaft. The driven wheel (16) is meshed with the driving wheel (15). The upper end of the rotating shaft is coaxially mounted on the lower end surface of the centrifugal disc (2).

3. The microbial automated separation and cultivation equipment according to claim 1, characterized in that: The second driving component comprises: A cylinder (17), wherein the cylinder (17) is mounted on the upper end surface of the centrifugal disc (2); A lifting frame (18), the lifting frame (18) is installed on the output end of the cylinder (17), and a plurality of bracket plates extend from the side end of the lifting frame (18); A fixing seat (19), wherein the fixing seat (19) is installed inside the centrifugal disc (2); A support rod (20), the lower end of which is mounted on the bracket plate, and the support rod (20) is also slidably mounted on the fixing seat (19); A slider (21), wherein the slider (21) is hinged to the upper end of the support rod (20); A support platform (22), wherein the support platform (22) is fixedly mounted on the fixing seat (19); A guide rod (23), one end of the guide rod (23) is hinged on the support platform (22), the sedimentation trough (8) is fixedly connected to the end of the guide rod (23), the other end of the guide rod (23) is clamped on the valve cover (9), and the slider (21) is also slidably arranged on the guide rod (23).

4. The microbial automated separation and cultivation equipment according to claim 1, characterized in that: The opening and closing member comprises a reed (24) and a closing piece (25), wherein the reed (24) is fixedly mounted on the inner wall of the hemispherical bottom of the sedimentation trough (8), and the closing piece (25) is mounted on the end of the reed (24), and the closing piece (25) covers the discharge opening (11).

5. The microbial automated separation and cultivation equipment according to claim 3, characterized in that: The material taking component comprises: A flow diverter seat (26), the flow diverter seat (26) is fixedly mounted on the centrifugal disk (2), a centrifugal channel (27) is provided inside the flow diverter seat (26), a docking port (28) is provided at a side end of the culture medium (6), and the docking port (28) is in communication with the centrifugal channel (27); a flip arm (29), the material taking head (12) being mounted on the flip arm (29), the lower end of the flip arm (29) being hinged on the diverter seat (26), the material taking head (12) being connected to the centrifugal channel (27) through the cavity structure of the flip arm (29), and an inclined slot (30) being provided on the flip arm (29); A sliding rod (31), the sliding rod (31) is slidably disposed on the diverter seat (26), and the lower end of the sliding rod (31) is connected to the lifting frame (18); A sliding head (32), wherein the middle portion of the sliding head (32) is mounted on the top end of the sliding rod (31), and both ends of the sliding head (32) are slidably disposed in the inclined groove (30); A pressure pump (33), wherein the pressure pump (33) is installed at the upper end of the diverter seat (26), and the working end of the pressure pump (33) is connected to the centrifugal channel (27).

6. The microorganism automated separation and cultivation equipment according to claim 5, characterized in that: There are at least two flip arms (29). After the ends of the two flip arms (29) are flipped close to each other, the material taking head (12) extends into the sedimentation bottom tank (8) through the discharge opening (11), and the opening and closing member is in an open state.

7. The microorganism automated separation and cultivation equipment according to claim 1, characterized in that: Both ends of the sedimentation bottom tank (8) are provided with elastic clamping pieces (34), and the clamping ends of the elastic clamping pieces (34) match with the outer end surface of the storage tube (7).

8. The microorganism automated separation and cultivation equipment according to claim 3, characterized in that: The fixing seat (19) is also provided with a stabilizing portion for positioning the valve cover (9), the stabilizing portion comprising: A guide rod (35), wherein the guide rod (35) is mounted on the fixing seat (19); A mounting block (36), wherein the mounting block (36) is slidably disposed on the guide rod (35); a spring (37), wherein the spring (37) is sleeved on the guide rod (35), one end of the spring (37) abuts against the mounting block (36), and the other end of the spring (37) abuts against the fixing seat (19); A clamping plate (38) is mounted on the upper end of the mounting block (36), and the edge end of the valve cover (9) is clamped on the clamping plate (38).

9. The microorganism automated separation and cultivation equipment according to claim 1, characterized in that: A plurality of positioning blocks (39) are circumferentially mounted on the inner wall of the top cover (3), and a plurality of positioning blocks (39) are provided with positioning grooves (40), and a plurality of positioning grooves (40) are matched with the outer wall of the storage cylinder (7).

10. The microorganism automated separation and cultivation equipment according to claim 9, characterized in that: A side end of the valve cover (9) is provided with an avoidance groove (41), and the groove depth of the avoidance groove (41) is not less than the extended length of the positioning groove (40).

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