Bacillus velezensis YPTJ-1 strain as well as microbial powder and bacterial fertilizer prepared from bacillus velezensis YPTJ-1 strain

Abstract

The bacillus velezensis YPTJ-1 strain is preserved in the China General Microbiological Culture Collection Center on July 11, 2024, the preservation number is CGMCC No.31271, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing. The bacillus velezensis strain is subjected to fermentation treatment, and the microbial powder is obtained based on the product of the fermentation treatment. The microbial fertilizer containing bacillus velezensis microbial powder comprises 70%-86% of rape straw organic fertilizer, 8%-10% of calcium magnesium phosphate fertilizer, 3%-5% of manganese sulfate and 3%-15% of microbial powder, and the sum of the mass percentages of all the raw materials is 100%. The granular microbial fertilizer is prepared by mixing the mixture of the raw materials with the oxidized starch adhesive and then granulating. The quinoa basal stem rot can be effectively treated by adopting the microbial fertilizer.

Description

Technical Field

[0001] The invention relates to the field of agricultural biotechnology, in particular to a Bacillus Velez YPTJ-1 strain and microbial powder and bacterial fertilizer prepared therefrom. Background Art

[0002] Quinoa stem rot is caused by Fusarium solani ( Fusarium solani ) is a soil-borne disease caused by infection, which harms the root system and stem base of quinoa. The affected plants will show leaf chlorosis, shedding, lodging, and reduced yield. There are no ideal prevention and control measures at home and abroad. Quinoa is an important grain crop in Shanxi Province. Due to years of continuous planting and the dry and high temperature environment in the greenhouse, the incidence of quinoa stem base rot has become more serious year by year. Surveys show that the incidence rate of quinoa stem base rot is about 70% in general years. In severe cases, it can cause quinoa yield losses of up to 100%. In addition, F. solani It belongs to a type of toxic fungus that may contaminate quinoa seeds, which not only affects human health, but has also become a key factor restricting quinoa yield and quality. At present, there are no pesticides for quinoa stem rot in my country's pesticide market, and the long-term use of traditional chemical agents will not only cause "3R" problems, but also damage the soil structure. Therefore, it is urgent to seek a new prevention and control method.

[0003] Microbial fertilizer refers to the use of living microorganisms and their metabolites to prevent and control agricultural pests and diseases. It is not only green and pollution-free, but also has the advantages of improving the soil ecological environment and inhibiting soil-borne diseases. The mechanism of action is mainly to inhibit pathogens and activate the soil through the powerful reproduction and competitiveness of living bacteria. Among the numerous antagonistic strains of biocontrol, the genus Bacillus is one of the dominant microbial populations in the soil. Because it can produce spores, it has strong resistance to stress and antibacterial and disease prevention effects. Compared with other types of biocontrol bacteria, bacterial Bacillus is more conducive to the production of dosage forms, dosage form processing, and survival, colonization and reproduction in the environment.

[0004] Bacillus Velez is an aerobic or facultative anaerobic spore-forming Gram-positive bacterium with rich and diverse physiological characteristics. It is widely present in nature and is an important microorganism in the soil and rhizosphere of plant surfaces. It can produce antagonistic substances to inhibit the growth of harmful bacteria or kill pathogens, and can also improve soil nutrient activity, promote plant growth, and improve the quality of agricultural products, ultimately achieving the goal of increasing crop yields. This study made beneficial bacteria into microbial fertilizers and released them into the soil, which integrates growth promotion, occupation, antagonism, and immune induction, which is of great significance for preventing and controlling quinoa stem base rot and improving the economic benefits of quinoa. Summary of the invention

[0005] In order to solve the above problems existing in quinoa production, the present invention provides a Bacillus Velez YPTJ-1 strain and microbial powder and bacterial fertilizer prepared therefrom.

[0006] The present invention adopts the following technical solution: Bacillus Velez Bacillus velezensis ) YPTJ-1 strain, deposited at the General Microbiology Center of China Microorganism Culture Collection on July 11, 2024, with the deposit number CGMCC No.31271, and the deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0007] Using Bacillus velez Bacillus velezensis ) The microbial powder of the YPTJ-1 strain is obtained by fermenting the Velezac bacteria strain and obtaining the microbial powder based on the fermentation product.

[0008] A microbial fertilizer containing microbial powder includes the following raw materials in percentage by mass: 70%-86% rape straw organic fertilizer, 8%-10% calcium magnesium phosphate fertilizer, 3%-5% manganese sulfate, and 3%-15% microbial powder, and the sum of the percentage by mass of the above raw materials is 100%; a mixture of the above raw materials is mixed with an oxidized starch adhesive and then granulated to form a granular microbial fertilizer, wherein the microbial fertilizer contains an effective live bacteria count of ≥200 million CFU / g.

[0009] Furthermore, the oxidized starch adhesive comprises the following raw materials in percentage by mass: 50%-60% corn starch, 30% hydrogen peroxide 4%-8%, 30% NaOH solution 8%-10%, 3%-5% FeSO4 powder, 2%-4% borax, 1%-3% sodium thiosulfate, 2%-4% tributyl phosphate, 7%-10% urea and 6%-15% bentonite.

[0010] Furthermore, the mass ratio of the mixture of the raw materials to the oxidized starch adhesive is 1000:5.

[0011] A method for treating quinoa stem base rot comprises adding 4-6 g of microbial fertilizer granules to each kilogram of quinoa seeds for seed mixing; or adding 5-10 kg of granules per mu of land during tillage, wherein the granules are evenly mixed with soil at a mass ratio of 1:8-10 and then added to a tillage machine.

[0012] Compared with the prior art, the present invention has the following beneficial effects: The microbial fertilizer prepared by Bacillus Velez YPTJ-1 of the present invention not only has an antagonistic effect on quinoa stem base rot pathogens, but is also a beneficial soil bacterium. The microbial fertilizer of the present invention can prevent and treat soil-borne diseases caused by soil-dwelling fungi such as Fusarium solani, Fusarium graminearum, Pseudo-Fusarium graminearum, and Pseudo-Verticillium fusarium, while increasing the diversity of microorganisms in the soil, balancing the soil microecology, thereby enhancing the absorption and utilization efficiency of quinoa root nutrients, increasing root vitality, further increasing quinoa yield, and improving quality.

[0013] The microbial fertilizer of the invention has simple preparation process, is easy to operate, has undemanding fermentation conditions, and has simple raw materials with wide sources and low cost for industrial production. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The colony morphology of Bacillus Velez YPTJ-1 of the present invention on NA medium; Figure 2 This is the Gram staining image of Bacillus velez YPTJ-1; Figure 3 This is a scanning electron micrograph of strain YPTJ-1; Figure 4 The phylogenetic tree of Bacillus Velez YPTJ-1 of the present invention based on the 16S rDNA gene sequence; Figure 5 A phylogenetic tree of Bacillus Velez YPTJ-1 of the present invention based on the 16S gyrA gene sequence; Figure 6 is a phylogenetic tree of Bacillus Velez YPTJ-1 of the present invention based on the 16S gyrB gene sequence; Figure 7 The inhibitory effect of Bacillus Velez YPTJ-1 on Fusarium solani; Figure 8 The inhibitory effect of Bacillus Velez YPTJ-1 on Fusarium graminearum; Fig. 9 The inhibitory effect of Bacillus Velez YPTJ-1 on Fusarium graminearum; Fig.10 The inhibitory effect of Bacillus Velez YPTJ-1 on Fusarium spp. Fig.11 The growth-promoting effect of strain YPTJ-1 on quinoa seeds; Fig.12 The effect of strain YPTJ-1 on the growth of quinoa; Fig.13 This is the physiological and biochemical mechanism of strain YPTJ-1 in promoting plant growth.

[0015] in, Fig.11 In the table, A: CK; B: 1X; C: 10X; D: 50X; E: 100X; F: 200X; G: 400X; H: 1000X, where X is the dilution multiple of the fermentation broth; Fig.12 In the figure, A: seedling growth; B: germination rate; C: plant height, root length; D: fresh weight and dry weight of aboveground parts; E: stem diameter; F: fresh weight and dry weight of underground parts; G: leaf area; H: number of leaves; Fig.13 In the figure, A: nitrogen fixation; B: organic phosphorus solubilization; C: inorganic phosphorus solubilization; D: potassium solubilization; E: iron-loving; F: NB culture medium + colorimetric solution; G: NB fermentation supernatant + colorimetric solution; H: NB culture medium containing L-tryptophan + colorimetric solution; I: NB fermentation supernatant containing L-tryptophan + colorimetric solution. DETAILED DESCRIPTION

[0016] Bacillus Velez Bacillus velezensis ) YPTJ-1 strain, deposited at the General Microbiology Center of China Microorganism Culture Collection on July 11, 2024, with the deposit number CGMCC No.31271, and the deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0017] The Bacillus velez YPTJ-1 was isolated from wild grapes ( Vitis flexuosa When the strain grows on NA medium, it forms white, round, dry-surface, moist-inside, wrinkled, slightly convex and opaque colonies, such as Figure 1 Gram staining is positive, and microscopic observation shows that the bacteria are rod-shaped, such as Figure 2 Scanning electron microscopy also showed that the bacteria were rod-shaped, such as Figure 3 , the size is approximately (4.2~6.1)μm×(0.6~0.8)μm.

[0018] The 16S rDNA sequence was compared and it was found that strain YPTJ-1 was similar to Bacillus Bacillus sp., Brevibacterium Brevibacterium sp., Bacillus simplex Bacillus simplex , Bacillus thuringiensis Bacillus thuringiensis The homologous sequence similarity is as high as 100%, and it is impossible to distinguish which species it is. Figure 4 . The strain YPTJ-1 was gyr A and gyr B. velez Bacillus velezensis The homologous sequence similarity of gyr A and gyrPhylogenetic trees were constructed based on the B gene sequences and it was found that strain YPTJ-1 and Bacillus velez Bacillus velezensis All of them are clustered into one branch with 100% bootstrap support. Figure 5 , Figure 6 , different from other species of Bacillus, comprehensive morphological characteristics and phylogenetic analysis identified strain YPTJ-1 as Bacillus velezensis Bacillus velezensis .

[0019] Bacillus Velez YPTJ-1 has good preventive effects on soil-borne diseases caused by Fusarium solani, Fusarium graminearum, Pseudo-Fusarium graminearum and Pseudomonas syringae, especially against Fusarium solani that causes quinoa stem rot. It has high inhibitory activity against Fusarium solani that causes quinoa stem rot. It is combined with relevant additives to make microbial fertilizer, which not only has a significant growth-promoting effect on quinoa plants, but also has a significant preventive and therapeutic effect on quinoa stem rot.

[0020] The present invention provides a method for preparing microbial fertilizer, and the test data confirms that YPTJ-1 microbial fertilizer has good prevention and control effects and growth promotion effects. It comprises the following steps: 1. Inoculate and culture strain YPTJ-1 to obtain bacterial suspension; YPTJ-1 bacterial suspension: After culturing Bacillus Velezii YPTJ-1 on NA medium for 48 h, a single colony was picked and inoculated into NB medium at 25°C and 160 r·min. -1 After shaking culture for 24 h, the seed solution was obtained. Then the YPTJ-1 seed solution was mixed with the NYBD culture solution at a volume ratio of 1:100 and incubated at 25°C and a rotation speed of 160 r·min -1 The fermentation liquid was obtained by shaking culture for 48 h under the same conditions. The spore concentration was calculated by plate spreading method and was 2×10 8 CFU·mL -1 ,spare.

[0021] NA medium formula 1: beef extract 5 g, peptone 10 g, NaCl 5 g, agar powder 20 g, glucose 20 g, distilled water 1000 mL, pH 7.0.

[0022] NB medium formula 2: beef extract 5 g, peptone 10 g, NaCl 5 g, glucose 20 g, distilled water 1000 mL, pH 7.0.

[0023] The formula of NYBD culture medium is: 8 g beef extract, 5 g yeast extract, 10 g glucose, 1000 mL distilled water, pH 7.0.

[0024] 2. Add the prepared bacterial suspension into wheat bran, ferment, dry and crush to obtain Bacillus Velez YPTJ-1 bacterial powder; 3. Preparation of microbial fertilizer (1) Take the following raw materials in the following mass percentages: 86% of fully fermented rape straw organic fertilizer, 8% of calcium magnesium phosphate fertilizer, 3% of manganese sulfate, and 3% of microbial powder, and mix the raw materials evenly; (2) The oxidized starch adhesive includes the following raw materials in percentage by mass: 50% corn starch, 4% 30% hydrogen peroxide, 8% 30% NaOH solution, 5% FeSO4 powder, 3% borax, 1% sodium thiosulfate, 4% tributyl phosphate, and 25% drying agent (10% urea, 15% bentonite), which are mixed evenly. The fineness of the powdered raw materials is 100-200 mesh.

[0025] The preparation method of oxidized starch adhesive comprises the following steps: Place the reactor in a water bath, add warm water into the reactor, then add corn starch and FeSO4 powder, and stir to form a uniform slurry; add hydrogen peroxide and a small amount of NaOH solution into the reactor, and stir continuously to fully oxidize the corn starch at an oxidation temperature of 60°C and an oxidation time of 40 min; add the remaining NaOH solution to fully gelatinize the oxidized starch; add urea and bentonite, and stir evenly; add borax, sodium thiosulfate and tributyl phosphate, continue stirring for a while, then make up the volume and cool to room temperature to obtain an auxiliary additive for microbial fertilizer disc granulation.

[0026] (3) taking the raw material mixture and the oxidized starch adhesive in a mass ratio of 1000:5; (4) The raw material mixture is placed in a disc granulator, oxidized starch adhesive is added, and disc granulation is performed to obtain granular microbial fertilizer.

[0027] Take the microbial fertilizer, measure various data, and compare with the indicators in the implementation standard. The results are shown in Table 1.

[0028] Table 1 Microbial fertilizer determination data It can be seen from the data in Table 1 that the YPTJ-1 microbial fertilizer obtained in this implementation meets the requirements in all its indicators.

[0029] Verification test 1: Bacillus velez ( B. velezensis )Bacteriostasis test of YPTJ-1 strain The four-point plate confrontation method was used. A 5 mm diameter fungus cake was punched on the edge of the plate of Fusarium solani, Fusarium graminearum, Pseudo-Fusarium graminearum and Pseudo-Fusarium verticillium with a hole puncher, and then planted in the center of the solid PDA plate for culture. The isolated and purified YPTJ-1 strain was inoculated symmetrically on the four sides 20 mm away from it. Only the pathogenic fungus was inoculated as a control. Each experiment was repeated 3 times. After inverted culture at 25℃ for 6 days, the diameter of the pathogen was measured by the cross method, and the inhibition rate was calculated.

[0030] The inhibition rates of Bacillus Velezii YPTJ-1 against Fusarium solani, Fusarium graminearum, Pseudomonas graminearum and Fusarium pseudoverticillium were 84.49%, 71.39%, 71.45 and 65.22% respectively ( Figure 7-10 ).

[0031] Verification test 2: Bacillus velez ( B. velezensis ) YPTJ-1 strain growth promotion test (1) Seed germination test Select plump and uniform quinoa seeds, disinfect the surface, and use sterile water to prepare stock solution, 10-fold fermentation solution, 50-fold fermentation solution, 100-fold fermentation solution, 200-fold fermentation solution, 400-fold fermentation solution, and 1000-fold fermentation solution. Place a sterile filter paper in a 90 mm culture dish, add different treatment solutions to the filter paper, keep it moist, use uninoculated NB as a control, put 20 quinoa seeds in each dish, set up three replicates, and culture in a constant temperature incubator at 25°C. Observe the germination situation every day, investigate and record the number of seed germinations, and consider the germinated seeds when the radicle length is half the length of the seeds. Calculate the effects of strain YPTJ-1 on the germination potential, germination rate, germination index, and seed vigor coefficient of the seeds ( Fig.11 , Table 2).

[0032] Table 2 Effect of strain YPTJ-1 on promoting germination of quinoa seeds Fig.11 As shown in Table 2, under the treatment of soaking seeds in different concentrations of bacterial solution, strain YPTJ-1 has different effects on the germination of quinoa seeds. The fermentation liquid diluted below 400 times has an inhibitory effect on quinoa seeds, while the fermentation liquid diluted 1000 times has a good promoting effect on the germination and growth of quinoa seeds.

[0033] (2) Determination of the effect of strain YPTJ-1 on plant growth Quinoa seeds with consistent growth were selected and placed in a culture dish. Sterile water was used to prepare the bacterial solution into 400-fold fermentation solution and 1000-fold fermentation solution respectively. Sterile water was used as the control group. After soaking the seeds for 3 hours, they were sown in seedling pots, with 10 test quinoa seeds in each pot. After 7 days, different treatment solutions were used to irrigate the roots of different treatment groups. Each pot was irrigated with 20 mL, and irrigation was carried out every 7 days. Each experiment was repeated 3 times and irrigated continuously for 5 times. 7 days after the last application of the bacterial solution, the effects of strain YPTJ-1 on the stem diameter, leaf area, number of leaves, plant height, root length, emergence rate, fresh weight (aboveground and underground parts), and dry weight (aboveground and underground parts) of quinoa plants were determined.

[0034] After treatment with YPTJ-1 fermentation liquid diluted 1000 times, the germination rate of quinoa seeds was significantly different at 24 h, but there was no difference in germination rate among the three groups at 48 h ( Fig.12 B in the figure). At 42 days, compared with the control, when the fermentation liquid was diluted 400 times, the underground fresh weight and leaf area of ​​quinoa seedlings were significantly different, while the root length, plant height, aboveground fresh weight, dry weight, underground dry weight, and leaf number were not significantly different ( Fig.12 ). When the dilution ratio of the fermentation liquid was 1000 times, the above 10 indicators were significantly higher than those of the control group. The experimental results showed that when the fermentation liquid of strain YPTJ-1 was 400 times, the measured indicators were close to those of the control group, and when the fermentation liquid was 1000 times, it had a significant promoting effect on the growth of quinoa.

[0035] (3) Preliminary study on the growth-promoting mechanism of strain YPTJ-1 The growth-promoting ability of strain YPTJ-1 was qualitatively tested, including the ability to fix nitrogen, solubilize phosphorus, solubilize potassium, be iron-loving and produce IAA.

[0036] like Fig.11 , Fig.12 As shown, strain YPTJ-1 has a certain promoting effect on quinoa seed germination and seedling growth. Analysis of the physiological and biochemical mechanism of the growth-promoting effect of strain YPTJ-1 revealed that the strain has the ability to fix nitrogen and decompose inorganic phosphorus ( Fig.13 AC), and IAA ( Fig.13 medium FI), but no ability to degrade organic phosphorus, potassium and iron ( Fig.13 B, D, and I in the middle). These results indicate that the growth-promoting ability of strain YPTJ-1 is caused by nitrogen fixation, inorganic phosphorus decomposition, and IAA production.

[0037] Verification test 3: Microbial fertilizer potted plant test The seeds of "Jiaqi 100" with the same growth potential were selected, and the greenhouse growth promotion test was carried out by soaking seeds and mixing with soil. Seed soaking treatment: The seeds were placed in a culture dish, 2 mL of the corresponding treatment solution was added, YPTJ-1 microbial fertilizer was used as treatment 1, Bacillus subtilis was used as treatment 2, and sterile water was used as a blank control, and soaked for 5 hours. Then sowed in seedling pots. Soil mixing treatment: The substrate was mixed with the corresponding treatment fertilizer and placed in a seedling pot (diameter 11 cm, bottom diameter 8 cm, pot height 10 cm), 8 seeds per pot, and each treatment was repeated 3 times. When the seedlings grew to two leaves and one heart, the corresponding treatment fertilizer was irrigated every 7 days. The sterile water treatment was also used as a common control for the above two. The seedling emergence rate, root length, root fresh weight, total fresh weight and dry weight were measured after 35 days.

[0038] Table 4 Effects of different treatments on the growth of quinoa seedlings Under different treatments (Table 4), the germination rate, root length, fresh weight and dry weight of quinoa were different, among which the effect of YPTJ-1 microbial fertilizer was better than that of Bacillus subtilis and blank control.

[0039] Verification experiment 4: Field test of microbial fertilizer The obtained microbial fertilizer was subjected to field tests to identify the disease prevention and growth promotion effects of microbial preparations on quinoa stem rot. The field test was conducted in Jingle County, Shanxi Province, and plots with relatively uniform fertility, flat terrain, and a high incidence of stem rot in the past were selected as test fields. Microbial fertilizer was applied as base fertilizer before sowing or during land preparation, with an application rate of 10kg / mu. After quinoa entered the middle and late stages, topdressing was performed once, and generally root irrigation was performed when quinoa grew to 40-50 cm, with an application rate of 10kg / mu.

[0040] The experiment was set up with three treatments, randomized in blocks, repeated three times, and the plot area was 200m 2 . The planting density was 8000 to 10000 plants / mu, and the row spacing was controlled at 30-50 cm and the plant spacing was 10-20 cm. Treatment group: YPTJ-1 microbial fertilizer; fertilizer control group: Bacillus subtilis microbial fertilizer (commercially available) with the same application method and dosage as the treatment group; blank control group: clean water with the same application method and dosage as the treatment group. The field disease rate of quinoa was investigated in the middle and late stages of its growth, and the thousand-grain weight and yield per unit area were investigated at harvest. The field control effects of each treatment on quinoa stem base rot are shown in Table 5, and the yield-increasing effects of each treatment on quinoa are shown in Table 6.

[0041] Table 5 Disease rate of quinoa plants under each treatment The diseased plant rate of YPTJ-1 microbial fertilizer was the lowest, which was significantly different from the field diseased plant rate of the fertilizer control and blank control.

[0042] Table 6 Yield determination of quinoa under different treatments There were great differences in the thousand-grain weight of quinoa under different treatments, and the YPTJ-1 microbial fertilizer was significantly higher than other treatments. The per-acre yield measurement results showed that the per-acre yield of quinoa treated with YPTJ-1 microbial fertilizer was the largest, reaching 817.94 kg, which was significantly higher than the fertilizer control and blank control.

[0043] Verification test 5: YPTJ-1 microbial fertilizer field test 2 In 2022, in Peng Village, Yuci District, Jinzhong City, Shanxi Province, a field test of quinoa prepared by the YPTJ-1 microbial fertilizer of the present invention was conducted. The test treatment was the YPTJ-1 microbial fertilizer of the present invention, and the control was the commercially available general microbial fertilizer (Inner Mongolia Lineng Fertilizer Industry Biotechnology Co., Ltd.), compound fertilizer (Stanley Agricultural Group Co., Ltd.) and clean water treatment. The test was repeated three times, and the area of ​​the plot was 1 mu: fertilization was applied 3 times during the growth of quinoa, the first time as base fertilizer, the second and third times as topdressing, and each fertilization was applied according to the standard of 150kg / mu. Only the fertilization is different, and other management and seedling cultivation are exactly the same. The growth situation, disease index, etc. were counted separately, the use effect of each fertilizer was calculated, and the yield was investigated after harvest (Table 9).

[0044] Table 9 Effect of applying YPTJ-1 microbial fertilizer on quinoa By analyzing different indicators of quinoa yield, the results showed that after treatment with YPTJ-1 microbial fertilizer, quinoa yield was significantly higher than that of other treatments. The yield per mu reached 846.04 kg after application of YPTJ-1 microbial fertilizer, and the disease index was also significantly lower than that of other treatment groups. After treatment with YPTJ-1 microbial fertilizer, the disease index was only 16.45%, indicating that YPTJ-1 microbial fertilizer has a good growth-promoting effect while also having a good prevention and control effect.

Claims

1. Bacillus Velezii ( Bacillus velezensis ) YPTJ-1 strain, deposited at the General Microbiology Center of China Microorganism Culture Collection on July 11, 2024, with the deposit number CGMCC No.31271, and the deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

2. Using the Bacillus Velezii as claimed in claim 1 ( Bacillus velezensis ) Microbial powder of YPTJ-1 strain, characterized in that: The Velez Bacillus strain is subjected to fermentation treatment, and microbial powder is obtained based on the fermentation product.

3. The microbial powder according to claim 2, characterized in that: The microbial powder is in powder form, and the number of viable microorganisms in the microbial powder is ≥ 1 billion CFU / g.

4. A microbial fertilizer comprising the microbial powder according to claim 2, characterized in that: The invention comprises the following raw materials in percentage by mass: 70%-86% of rape straw organic fertilizer, 8%-10% of calcium magnesium phosphate fertilizer, 3%-5% of manganese sulfate, and 3%-15% of microbial powder, and the sum of the percentage by mass of the above raw materials is 100%; a mixture of the above raw materials is mixed with an oxidized starch adhesive and granulated into granular microbial fertilizer, wherein the effective live bacteria count in the microbial fertilizer is ≥200 million CFU / g.

5. The microbial fertilizer according to claim 4, characterized in that: The oxidized starch adhesive comprises the following raw materials in percentage by mass: 50%-60% corn starch, 4%-8% 30% hydrogen peroxide, 8%-10% 30% NaOH solution, 3%-5% FeSO4 powder, 2%-4% borax, 1%-3% sodium thiosulfate, 2%-4% tributyl phosphate, 7%-10% urea and 6%-15% bentonite.

6. The microbial fertilizer according to claim 4 or 5, characterized in that: The mass ratio of the mixture of the raw materials to the oxidized starch adhesive is 1000:

5.

7. A method for treating quinoa stem base rot, using the microbial fertilizer prepared as claimed in claim 5 or 6, characterized in that: Add 4-6g of microbial fertilizer granules to every kilogram of quinoa seeds for seed mixing; or add 5-10kg of granules per mu of land when tilling the land. The method of adding is to mix the granules with soil in a mass ratio of 1:8-10 and then add them to the tiller.

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