Biological specimen preserving fluid for teaching and preparation method thereof

Abstract

The invention relates to the technical field of specimen preservation, in particular to biological specimen preservation liquid for teaching and a preparation method thereof. The biological specimen preserving fluid for teaching consists of the following raw materials in parts by weight: 2-6 parts of a preservative, 8-10 parts of glycerol, 3-5 parts of polyethylene glycol PEG-400, 5-8 parts of a functional additive, 4-7 parts of a synergistic additive, 3-5 parts of vitamin C and 30-40 parts of water, the biological specimen preserving fluid for teaching prepared by the invention not only can maintain the integrity of the morphological structure of a specimen and the original color of the specimen, but also has an excellent antibacterial effect, and effectively ensures the quality of the specimen.

Description

Technical Field

[0001] The invention relates to the technical field of specimen preservation, in particular to a biological specimen preservation solution for teaching and a preparation method thereof. Background Art

[0002] In the field of education, especially in biology teaching, biological specimens are indispensable intuitive teaching tools. They can help students understand the key points of knowledge such as the morphological structure, tissue characteristics and biodiversity of organisms more clearly and intuitively. Whether it is anatomical specimens of animals and plants or slide specimens of microorganisms, they play an important role in classroom teaching, experimental teaching and popular science exhibitions.

[0003] The traditional formalin solution, whose main component formaldehyde has strong bactericidal and preservative ability, can effectively prevent the corruption and degradation of biological tissues, so that specimens can be preserved for a long time. However, formaldehyde is a toxic and harmful volatile substance with a strong pungent odor. In the teaching environment, long-term exposure to the formaldehyde gas volatilized by formalin will irritate the respiratory tract and eyes of teachers and students, causing adverse reactions such as coughing, tearing, allergies, and may even cause cancer, seriously endangering the health of teachers and students. Some preservation solutions use quaternary ammonium salt compounds such as benzalkonium chloride, which have certain antibacterial properties, but they are still insufficient in long-term preservation effect, protection of specimen color and texture, and biocompatibility.

[0004] Based on this, the present invention provides a biological specimen preservation solution for teaching and a preparation method thereof to solve the above-mentioned technical problems. Summary of the invention

[0005] The purpose of the present invention is to provide a biological specimen preservation solution for teaching and a preparation method thereof. The prepared biological specimen preservation solution for teaching can not only maintain the integrity of the specimen's morphological structure and its original color, but also has an excellent antibacterial effect, effectively ensuring its quality.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] The first aspect of the present invention provides a biological specimen preservation solution for teaching, which is composed of the following raw materials in parts by weight: 2 to 6 parts of preservatives, 8 to 10 parts of glycerol, 3 to 5 parts of polyethylene glycol PEG-400, 5 to 8 parts of functional additives, 4 to 7 parts of synergistic additives, 3 to 5 parts of vitamin C and 30 to 40 parts of water.

[0008] The present invention is further configured as follows: the preservative is formed by mixing sodium benzoate and potassium sorbate in a mass ratio of 1:0.46-0.52.

[0009] The present invention is further configured as follows: the preparation process of the functional additive is as follows:

[0010] The composite powder of 60-80 mesh is placed in an ethanol aqueous solution with a volume concentration of 95% and soaked for 12-14 hours at a dosage ratio of 0.02-0.06 g / mL;

[0011] Then, ultrasonic extraction is performed, and the extract is centrifuged at 9000-10000 r / min for 30-35 minutes, and the supernatant is taken and sterilized to obtain a functional additive.

[0012] The present invention is further configured as follows: the composite powder is mixed with clove powder and licorice powder in a mass ratio of 1:0.2-0.4.

[0013] The present invention is further configured as follows: the temperature of the ultrasonic extraction is 30-60° C., the power is 100-300 W, and the time is 50-60 min.

[0014] The present invention is further configured as follows: the preparation process of the synergistic aid is as follows:

[0015] Nano-titanium dioxide is placed in toluene for ultrasonic treatment at a dosage ratio of 0.12 to 0.24 g / mL for 20 to 30 minutes, 3-aminopropyltriethoxysilane is added thereto at a weight percentage of 5.2 to 6.2% of the mass of the nano-titanium dioxide, and stirred for 8 to 10 hours at 220 to 320 r / min in a nitrogen atmosphere, filtered, washed with anhydrous ethanol for 2 to 4 times, and vacuum dried to obtain a base material;

[0016] The base material is placed in a compound liquid at a dosage of 0.05 to 0.08 g / mL and subjected to ultrasonic treatment for 20 to 30 minutes, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide are added thereto, and stirred for 15 to 30 minutes at 250 to 350 r / min in a room temperature, and then the baicalin solution is added, and the stirring treatment is continued for 10 to 14 hours. After suction filtration, the mixture is washed with the compound liquid for 2 to 4 times, and vacuum dried to constant weight to obtain a synergistic auxiliary agent;

[0017] Wherein, the addition amount of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 2.5-4.5% of the base material mass;

[0018] The added amount of the N-hydroxysuccinimide is 3.2-4.6% of the mass of the base material.

[0019] The present invention is further configured as follows: the compound liquid is prepared by mixing sodium dihydrogen phosphate and disodium hydrogen phosphate in a volume ratio of 0.65 to 0.72:1.

[0020] The present invention is further configured as follows: the baicalin solution is prepared by mixing baicalin and ethanol at a solid-liquid ratio of 0.1-0.5 g / mL, and the addition amount of the baicalin solution is 8-10% of the mass of the base material.

[0021] The second aspect of the present invention: also provides a method for preparing the above-mentioned biological specimen preservation solution for teaching, comprising the following steps: accurately weighing preservatives, glycerol, polyethylene glycol PEG-400, functional additives, synergistic additives, vitamin C and water, and putting each raw material into a mixing device for mixing and stirring to obtain a mixed solution; then successively undergoing inspection and metering and packaging steps to finally obtain a finished product of the biological specimen preservation solution for teaching.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] In the present invention, preservatives, glycerol, polyethylene glycol PEG-400, functional additives, synergistic additives, vitamin C and water are used as raw materials. Preservatives, glycerol, polyethylene glycol PEG-400, functional additives, synergistic additives, vitamin C and water are put into a mixing device for mixing and stirring to obtain a mixed solution; and then the mixture is successively inspected and packaged to finally obtain a finished product of biological specimen preservation solution for teaching. The biological specimen preservation solution for teaching prepared by the present invention can not only maintain the integrity of the morphological structure of the specimen and its original color, but also has an excellent antibacterial effect, effectively ensuring its quality. The biological specimen preservation solution for teaching and the preparation method thereof provided by the present invention have a broader market prospect and are more suitable for promotion. DETAILED DESCRIPTION

[0024] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example 1

[0026] This embodiment provides a biological specimen preservation solution for teaching, which is composed of the following raw materials in parts by weight: 2 parts of preservatives, 8 parts of glycerol, 3 parts of polyethylene glycol PEG-400, 5 parts of functional additives, 4 parts of synergistic additives, 3 parts of vitamin C and 30 parts of water.

[0027] Among them, the preservative is a mixture of sodium benzoate and potassium sorbate in a mass ratio of 1:0.46.

[0028] The preparation process of functional additives is as follows:

[0029] The 60-mesh composite powder was placed in a 95% ethanol aqueous solution at a dosage ratio of 0.02 g / mL and soaked for 12 h;

[0030] Then, ultrasonic extraction is performed, and the extract is centrifuged at 9000 r / min for 30 minutes, and the supernatant is taken and sterilized to obtain a functional additive.

[0031] Furthermore, the composite powder is prepared by mixing clove powder and licorice powder in a mass ratio of 1:0.2.

[0032] The temperature of ultrasonic extraction was 30°C, the power was 100W, and the time was 50min.

[0033] In addition, the preparation process of the synergist is as follows:

[0034] Nano-titanium dioxide was placed in toluene at a dosage ratio of 0.12 g / mL and subjected to ultrasonic treatment for 20 min. 3-aminopropyltriethoxysilane (5.2% by mass of the nano-titanium dioxide) was added thereto, and stirred for 8 h under a nitrogen atmosphere at 220 r / min. After suction filtration, the mixture was washed twice with anhydrous ethanol and dried in vacuo to obtain a base material.

[0035] The base material was placed in the composite liquid at a dosage of 0.05 g / mL and subjected to ultrasonic treatment for 20 min. 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added thereto, and stirred at 250 r / min for 15 min under room temperature. Then, the baicalin solution was added, and the stirring treatment was continued for 10. After suction filtration, the mixture was washed twice with the composite liquid and vacuum dried to constant weight to obtain a synergistic agent.

[0036] Wherein, the addition amount of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 2.5% of the mass of the base material;

[0037] The added amount of the N-hydroxysuccinimide is 3.2% of the mass of the base material.

[0038] In this embodiment, it should be noted that nano titanium dioxide was purchased from Hubei Huifu Nanomaterials Co., Ltd.

[0039] The compound solution is prepared by mixing sodium dihydrogen phosphate and disodium hydrogen phosphate in a volume ratio of 0.65:1.

[0040] The baicalin solution is prepared by mixing baicalin and ethanol at a solid-liquid ratio of 0.1 g / mL, and the added amount of the baicalin solution is 8% of the mass of the base material.

[0041] In addition, this embodiment also provides a method for preparing the above-mentioned biological specimen preservation solution for teaching, comprising the following steps: accurately weighing preservatives, glycerol, polyethylene glycol PEG-400, functional additives, synergistic additives, vitamin C and water, and putting each raw material into a mixing device for mixing and stirring to obtain a mixed solution; and then successively undergoing inspection and metering and packaging steps to finally obtain a finished product of the biological specimen preservation solution for teaching.

[0042] Example 2

[0043] The preparation method of the biological specimen preservation solution for teaching provided in this embodiment is basically the same as that in Example 1, except that: the specific raw material composition and the specific preparation method of the biological specimen preservation solution for teaching in this embodiment are different; the specific raw material composition and the specific preparation method of the biological specimen preservation solution for teaching in this embodiment are as follows:

[0044] A biological specimen preservation solution for teaching is composed of the following raw materials in parts by weight: 3 parts of preservatives, 9 parts of glycerol, 4 parts of polyethylene glycol PEG-400, 6 parts of functional additives, 5 parts of synergistic additives, 4 parts of vitamin C and 32 parts of water.

[0045] Among them, the preservative is a mixture of sodium benzoate and potassium sorbate in a mass ratio of 1:0.48.

[0046] The preparation process of functional additives is as follows:

[0047] The 80-mesh composite powder was placed in a 95% ethanol aqueous solution at a dosage ratio of 0.03 g / mL and soaked for 13 h;

[0048] Then, ultrasonic extraction was performed, and the extract was centrifuged at 9100 r / min for 31 min. The supernatant was taken and sterilized to obtain a functional additive.

[0049] Furthermore, the composite powder is prepared by mixing clove powder and licorice powder in a mass ratio of 1:0.3.

[0050] The temperature of ultrasonic extraction was 32°C, the power was 110W, and the time was 52min.

[0051] In addition, the preparation process of the synergist is as follows:

[0052] Nano-titanium dioxide was placed in toluene at a dosage ratio of 0.14 g / mL and ultrasonically treated for 22 min, 3-aminopropyltriethoxysilane (5.4% by mass of the nano-titanium dioxide) was added thereto, and stirred for 9 h under a nitrogen atmosphere at 230 r / min, and filtered, washed with anhydrous ethanol for 3 times, and vacuum dried to obtain a base material;

[0053] The base material was placed in the composite liquid at a dosage of 0.06 g / mL and ultrasonically treated for 22 minutes, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added thereto, and stirred for 16 minutes at 260 r / min in a room temperature, and then the baicalin solution was added, and the stirring treatment was continued for 11 hours. After suction filtration, the mixture was washed 3 times with the composite liquid, and vacuum dried to constant weight to obtain a synergistic additive;

[0054] Wherein, the addition amount of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 2.7% of the base material mass;

[0055] The added amount of the N-hydroxysuccinimide is 3.4% of the mass of the base material.

[0056] In this embodiment, it should be noted that nano titanium dioxide was purchased from Hubei Huifu Nanomaterials Co., Ltd.

[0057] The compound solution is prepared by mixing sodium dihydrogen phosphate and disodium hydrogen phosphate in a volume ratio of 0.67:1.

[0058] The baicalin solution is prepared by mixing baicalin and ethanol at a solid-liquid ratio of 0.2 g / mL, and the added amount of the baicalin solution is 9% of the mass of the base material.

[0059] In addition, this embodiment also provides a method for preparing the above-mentioned biological specimen preservation solution for teaching, comprising the following steps: accurately weighing preservatives, glycerol, polyethylene glycol PEG-400, functional additives, synergistic additives, vitamin C and water, and putting each raw material into a mixing device for mixing and stirring to obtain a mixed solution; and then successively undergoing inspection and metering and packaging steps to finally obtain a finished product of the biological specimen preservation solution for teaching.

[0060] Example 3

[0061] The preparation method of the biological specimen preservation solution for teaching provided in this embodiment is basically the same as that in Example 1, except that: the specific raw material composition and the specific preparation method of the biological specimen preservation solution for teaching in this embodiment are different; the specific raw material composition and the specific preparation method of the biological specimen preservation solution for teaching in this embodiment are as follows:

[0062] A biological specimen preservation solution for teaching is composed of the following raw materials in parts by weight: 4 parts of preservatives, 9 parts of glycerol, 4 parts of polyethylene glycol PEG-400, 7 parts of functional additives, 5 parts of synergistic additives, 4 parts of vitamin C and 35 parts of water.

[0063] Among them, the preservative is a mixture of sodium benzoate and potassium sorbate in a mass ratio of 1:0.49.

[0064] The preparation process of functional additives is as follows:

[0065] The 70-mesh composite powder was placed in a 95% ethanol aqueous solution at a dosage ratio of 0.04 g / mL and soaked for 13 h;

[0066] Then, ultrasonic extraction was performed, and the extract was centrifuged at 9500 r / min for 32 minutes. The supernatant was taken and sterilized to obtain a functional additive.

[0067] Furthermore, the composite powder is prepared by mixing clove powder and licorice powder in a mass ratio of 1:0.3.

[0068] The temperature of ultrasonic extraction was 33°C, the power was 200W, and the time was 55min.

[0069] In addition, the preparation process of the synergist is as follows:

[0070] Nano-titanium dioxide was placed in toluene at a dosage ratio of 0.18 g / mL and ultrasonically treated for 25 min, 3-aminopropyltriethoxysilane (5.7% by mass of the nano-titanium dioxide) was added thereto, and stirred for 9 h under a nitrogen atmosphere at 270 r / min, and filtered, washed with anhydrous ethanol for 3 times, and vacuum dried to obtain a base material;

[0071] The base material was placed in the composite liquid at a dosage of 0.06 g / mL and ultrasonically treated for 25 min, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added thereto, and stirred for 22 min at 300 r / min in a room temperature, and then the baicalin solution was added, and the stirring treatment was continued for 12 h. After suction filtration, the mixture was washed 3 times with the composite liquid, and vacuum dried to constant weight to obtain a synergistic agent;

[0072] Wherein, the addition amount of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 3.5% of the base material mass;

[0073] The added amount of the N-hydroxysuccinimide is 3.9% of the mass of the base material.

[0074] In this embodiment, it should be noted that nano titanium dioxide was purchased from Hubei Huifu Nanomaterials Co., Ltd.

[0075] The compound solution is prepared by mixing sodium dihydrogen phosphate and disodium hydrogen phosphate in a volume ratio of 0.68:1.

[0076] The baicalin solution is prepared by mixing baicalin and ethanol at a solid-liquid ratio of 0.3 g / mL, and the added amount of the baicalin solution is 9% of the mass of the base material.

[0077] In addition, this embodiment also provides a method for preparing the above-mentioned biological specimen preservation solution for teaching, comprising the following steps: accurately weighing preservatives, glycerol, polyethylene glycol PEG-400, functional additives, synergistic additives, vitamin C and water, and putting each raw material into a mixing device for mixing and stirring to obtain a mixed solution; and then successively undergoing inspection and metering and packaging steps to finally obtain a finished product of the biological specimen preservation solution for teaching.

[0078] Example 4

[0079] The preparation method of the biological specimen preservation solution for teaching provided in this embodiment is basically the same as that in Example 1, except that: the specific raw material composition and the specific preparation method of the biological specimen preservation solution for teaching in this embodiment are different; the specific raw material composition and the specific preparation method of the biological specimen preservation solution for teaching in this embodiment are as follows:

[0080] A biological specimen preservation solution for teaching is composed of the following raw materials in parts by weight: 5 parts of preservatives, 9 parts of glycerol, 4 parts of polyethylene glycol PEG-400, 7 parts of functional additives, 6 parts of synergistic additives, 4 parts of vitamin C and 38 parts of water.

[0081] Among them, the preservative is a mixture of sodium benzoate and potassium sorbate in a mass ratio of 1:0.51.

[0082] The preparation process of functional additives is as follows:

[0083] The 80-mesh composite powder was placed in a 95% ethanol aqueous solution at a dosage ratio of 0.05 g / mL and soaked for 13 h;

[0084] Then, ultrasonic extraction was performed, and the extract was centrifuged at 9800 r / min for 34 minutes. The supernatant was taken and sterilized to obtain the functional additive.

[0085] Furthermore, the composite powder is prepared by mixing clove powder and licorice powder in a mass ratio of 1:0.3.

[0086] The temperature of ultrasonic extraction was 50°C, the power was 280W, and the time was 58min.

[0087] In addition, the preparation process of the synergist is as follows:

[0088] Nano-titanium dioxide was placed in toluene at a dosage ratio of 0.22 g / mL and ultrasonically treated for 28 min, 3-aminopropyltriethoxysilane (6% by mass of the nano-titanium dioxide) was added thereto, and stirred for 9 h at 310 r / min in a nitrogen atmosphere, filtered, washed with anhydrous ethanol for 3 times, and vacuum dried to obtain a base material;

[0089] The base material was placed in the composite liquid at a dosage ratio of 0.07 g / mL and ultrasonically treated for 28 minutes, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added thereto, and stirred for 28 minutes at 340 r / min in a room temperature, and then the baicalin solution was added, and the stirring treatment was continued for 13 hours. After suction filtration, the mixture was washed three times with the composite liquid, and vacuum dried to constant weight to obtain a synergistic additive;

[0090] Wherein, the addition amount of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 4.4% of the base material mass;

[0091] The added amount of the N-hydroxysuccinimide is 4.5% of the mass of the base material.

[0092] In this embodiment, it should be noted that nano titanium dioxide was purchased from Hubei Huifu Nanomaterials Co., Ltd.

[0093] The compound solution is prepared by mixing sodium dihydrogen phosphate and disodium hydrogen phosphate in a volume ratio of 0.7:1.

[0094] The baicalin solution is prepared by mixing baicalin and ethanol at a solid-liquid ratio of 0.4 g / mL, and the added amount of the baicalin solution is 9% of the mass of the base material.

[0095] In addition, this embodiment also provides a method for preparing the above-mentioned biological specimen preservation solution for teaching, comprising the following steps: accurately weighing preservatives, glycerol, polyethylene glycol PEG-400, functional additives, synergistic additives, vitamin C and water, and putting each raw material into a mixing device for mixing and stirring to obtain a mixed solution; and then successively undergoing inspection and metering and packaging steps to finally obtain a finished product of the biological specimen preservation solution for teaching.

[0096] Example 5

[0097] The preparation method of the biological specimen preservation solution for teaching provided in this embodiment is basically the same as that in Example 1, except that: the specific raw material composition and the specific preparation method of the biological specimen preservation solution for teaching in this embodiment are different; the specific raw material composition and the specific preparation method of the biological specimen preservation solution for teaching in this embodiment are as follows:

[0098] A biological specimen preservation solution for teaching is composed of the following raw materials in parts by weight: 6 parts of preservatives, 10 parts of glycerol, 5 parts of polyethylene glycol PEG-400, 8 parts of functional additives, 7 parts of synergistic additives, 5 parts of vitamin C and 40 parts of water.

[0099] Among them, the preservative is a mixture of sodium benzoate and potassium sorbate in a mass ratio of 1:0.52.

[0100] The preparation process of functional additives is as follows:

[0101] The 80-mesh composite powder was immersed in an ethanol aqueous solution with a volume concentration of 95% for 14 hours at a dosage ratio of 0.06 g / mL;

[0102] Then, ultrasonic extraction is performed, and the extract is centrifuged at 10000 r / min for 35 minutes. The supernatant is taken and sterilized to obtain a functional additive.

[0103] Furthermore, the composite powder is prepared by mixing clove powder and licorice powder in a mass ratio of 1:0.4.

[0104] The temperature of ultrasonic extraction was 60°C, the power was 300W, and the time was 60min.

[0105] In addition, the preparation process of the synergist is as follows:

[0106] Nano-titanium dioxide was placed in toluene for ultrasonic treatment at a dosage ratio of 0.24 g / mL for 30 min, 3-aminopropyltriethoxysilane (6.2% by mass of the nano-titanium dioxide) was added thereto, and the mixture was stirred for 10 h at 320 r / min in a nitrogen atmosphere, filtered, washed with anhydrous ethanol for 4 times, and vacuum dried to obtain a base material;

[0107] The base material was placed in the composite liquid at a dosage of 0.08 g / mL and ultrasonically treated for 30 min, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added thereto, and stirred for 30 min at 350 r / min in a room temperature, and then the baicalin solution was added, and the stirring treatment was continued for 14 h. After suction filtration, the mixture was washed 4 times with the composite liquid, and vacuum dried to constant weight to obtain a synergistic additive;

[0108] Wherein, the addition amount of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 4.5% of the base material mass;

[0109] The added amount of the N-hydroxysuccinimide is 4.6% of the mass of the base material.

[0110] In this embodiment, it should be noted that nano titanium dioxide was purchased from Hubei Huifu Nanomaterials Co., Ltd.

[0111] The compound solution is prepared by mixing sodium dihydrogen phosphate and disodium hydrogen phosphate in a volume ratio of 0.72:1.

[0112] The baicalin solution is prepared by mixing baicalin and ethanol at a solid-liquid ratio of 0.5 g / mL, and the addition amount of the baicalin solution is 10% of the mass of the base material.

[0113] In addition, this embodiment also provides a method for preparing the above-mentioned biological specimen preservation solution for teaching, comprising the following steps: accurately weighing preservatives, glycerol, polyethylene glycol PEG-400, functional additives, synergistic additives, vitamin C and water, and putting each raw material into a mixing device for mixing and stirring to obtain a mixed solution; and then successively undergoing inspection and metering and packaging steps to finally obtain a finished product of the biological specimen preservation solution for teaching.

[0114] Performance test: The samples of biological specimen preservation solution for teaching provided in Examples 1 to 5 are respectively marked as Examples 1 to 5; and the relevant properties of the biological specimen preservation solution for teaching provided in Examples 1 to 5 are respectively tested as follows:

[0115] 1. Antibacterial test: The test method is to dilute each group of teaching biological specimen preservation solutions by 5 times with sterile water to make suspensions, and detect the inhibitory effects of different biological specimen preservation solutions.

[0116] 2. Morphology and color test: The test method is to place the black carp specimens in each group of teaching biological specimen preservation solution and observe the morphology and color of each specimen.

[0117] The obtained test data are recorded in Table 1 and Table 2 below:

[0118] Table 1 Antibacterial performance test results of each group

[0119] Group Escherichia coli (%) Staphylococcus aureus (%) Example 1 Group 99.6 99.9 Example 2 Group 99.5 99.8 Example 3 Group 99.4 99.7 Example 4 group 99.5 99.8 Example 5 group 99.6 99.8

[0120] Table 2 Test results of morphology and color performance of each group

[0121]

[0122]

[0123] By comparing and analyzing the relevant data in Table 1 and Table 2, it can be seen that the biological specimen preservation solution for teaching prepared by the present invention can not only maintain the morphological structure of the specimen intact and maintain its original color, but also has an excellent antibacterial effect, effectively ensuring its quality. This shows that the biological specimen preservation solution for teaching and the preparation method thereof provided by the present invention have a broader market prospect and are more suitable for promotion.

[0124] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0125] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A biological specimen preservation solution for teaching, characterized in that: The invention is composed of the following raw materials in parts by weight: 2-6 parts of preservatives, 8-10 parts of glycerol, 3-5 parts of polyethylene glycol PEG-400, 5-8 parts of functional additives, 4-7 parts of synergistic additives, 3-5 parts of vitamin C and 30-40 parts of water.

2. A biological specimen preservation solution for teaching according to claim 1, characterized in that: The preservative is prepared by mixing sodium benzoate and potassium sorbate in a mass ratio of 1:0.46-0.

52.

3. The biological specimen preservation solution for teaching according to claim 1, characterized in that: The preparation process of the functional additive is as follows: The composite powder of 60-80 mesh is placed in an ethanol aqueous solution with a volume concentration of 95% and soaked for 12-14 hours at a dosage ratio of 0.02-0.06 g / mL; Then, ultrasonic extraction is performed, and the extract is centrifuged at 9000-10000 r / min for 30-35 minutes, and the supernatant is taken and sterilized to obtain a functional additive.

4. A biological specimen preservation solution for teaching according to claim 3, characterized in that: The composite powder is prepared by mixing clove powder and liquorice powder in a mass ratio of 1:0.2-0.

4.

5. The biological specimen preservation solution for teaching according to claim 3, characterized in that: The temperature of the ultrasonic extraction is 30-60° C., the power is 100-300 W, and the time is 50-60 min.

6. The biological specimen preservation solution for teaching according to claim 1, characterized in that: The preparation process of the synergistic aid is as follows: Nano-titanium dioxide is placed in toluene for ultrasonic treatment at a dosage ratio of 0.12 to 0.24 g / mL for 20 to 30 minutes, 3-aminopropyltriethoxysilane is added thereto at a weight percentage of 5.2 to 6.2% of the mass of the nano-titanium dioxide, and stirred for 8 to 10 hours at 220 to 320 r / min in a nitrogen atmosphere, filtered, washed with anhydrous ethanol for 2 to 4 times, and vacuum dried to obtain a base material; The base material is placed in a compound liquid at a dosage of 0.05 to 0.08 g / mL and subjected to ultrasonic treatment for 20 to 30 minutes, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide are added thereto, and stirred for 15 to 30 minutes at 250 to 350 r / min in a room temperature, and then the baicalin solution is added, and the stirring treatment is continued for 10 to 14 hours. After suction filtration, the mixture is washed with the compound liquid for 2 to 4 times, and vacuum dried to constant weight to obtain a synergistic auxiliary agent; Wherein, the addition amount of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 2.5-4.5% of the base material mass; The added amount of the N-hydroxysuccinimide is 3.2-4.6% of the mass of the base material.

7. A biological specimen preservation solution for teaching according to claim 6, characterized in that: The compound liquid is prepared by mixing sodium dihydrogen phosphate and disodium hydrogen phosphate in a volume ratio of 0.65 to 0.72:

1.

8. The biological specimen preservation solution for teaching according to claim 6, characterized in that: The baicalin solution is prepared by mixing baicalin and ethanol at a solid-liquid ratio of 0.1-0.5 g / mL, and the addition amount of the baicalin solution is 8-10% of the mass of the base material.

9. The method for preparing a biological specimen preservation solution for teaching according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: accurately weighing preservatives, glycerol, polyethylene glycol PEG-400, functional additives, synergistic additives, vitamin C and water, and putting the raw materials into a mixing device for mixing and stirring to obtain a mixed liquid; and then successively undergoing inspection and metering and packaging processes to finally obtain a finished product of a biological specimen preservation liquid for teaching.

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