Low-protein daily ration for later growth period of broiler chickens and application of low-protein daily ration
Through the response surface design, the amino acid requirement and balance mode of Ward 188 broilers in the late growth stage was determined, providing a low-protein diet with amino acid balance, solving the problem of amino acid imbalance in the diet of new domestic broilers, improving protein utilization and reducing nitrogen emissions.
Patent Information
- Application Number
- CN202510049080.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-13
- Publication Date
- 2025-03-14
AI Technical Summary
The nutritional needs and amino acid balance model of new domestic varieties of broilers in the late stage of growth are different from those of foreign commercial varieties, resulting in an imbalance in the proportion of amino acids in the diet, affecting growth performance and amino acid utilization efficiency, and increasing nitrogen emissions and environmental pollution.
Through response surface design, the requirements and balance modes of the restrictive amino acids arginine, valine and isoleucine are determined in the late stages of growth of Ward 188 broilers, providing a low-protein diet with amino acid balance, reducing diet protein levels and adding essential amino acids to maintain amino acid balance.
It improves the utilization rate and production performance of broiler egg whites, reduces nitrogen emissions and environmental pollution, and ensures the stability of broiler growth performance and slaughtering performance.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of animal feed preparation, and in particular relates to a low-protein diet for broiler chickens in the late growth stage and application thereof. Background Art
[0002] In the field of fast-growing large-sized white-feathered broiler breeding, my country's independently developed new white-feathered broiler breeds such as Ward 188 have made breakthrough progress, have passed variety approval, and have begun to be widely promoted and applied. However, the nutritional requirements and precise feed solutions for new domestic broiler breeds are still blank, limiting the development of their genetic potential.
[0003] Precision nutrition is an important means to achieve cost reduction, efficiency improvement, energy conservation and emission reduction in broiler farming. Low-protein diet technology is an important field of precision nutrition research at present. Low-protein diet refers to reducing the protein level of conventional diet by 2% to 4%. By adding synthetic amino acids, the animal's needs for essential amino acids and some functional amino acids are met. Dietary amino acid imbalance will lead to a decrease in animal protein utilization, which will lead to a decrease in its growth performance and an increase in nitrogen emissions, which will have an adverse effect on animal production and the environment; maintaining a balanced diet of amino acids can save feed protein resources such as soybean meal and reduce breeding emissions. However, in the actual production of new domestic broiler varieties, the growth curve is different from that of foreign commercial varieties, especially in the late growth period. There is a lack of precise scientific basis for the specific dietary amino acid requirements and their ratios; at the same time, the order of limiting amino acids and the amino acid balance pattern under low-protein diet conditions have also changed significantly, especially in the late growth period of broilers, when the growth rate is faster, and this variation is more obvious. At present, the market generally applies the nutritional standards of foreign commercial varieties, such as Ross 308, AA+, and Cobb broilers, which leads to an unbalanced amino acid ratio in the diet, seriously affecting the growth performance and amino acid utilization efficiency of domestic broilers, and reducing production efficiency. Studies have shown that under low-protein diet conditions, arginine and branched-chain amino acids have become limiting amino acids. Arginine is an essential amino acid for poultry. Poultry lacks carbamoyl phosphate synthetase and cannot synthesize arginine. Arginine is involved in the regulation of various physiological functions such as growth, reproduction, immunity, endocrine, and intestinal barrier. Branched-chain amino acids (valine, leucine, isoleucine) with different contents and proportions will affect the growth performance and carcass weight of broilers. Therefore, determining the required amount and balance pattern of these limiting amino acids in the late growth period of domestic broilers under low-protein diet conditions is the key to achieving precise nutrition and reducing costs and increasing efficiency.
[0004] Broiler nutrition and feed involve multiple influencing factors, and the effect of broiler products is also affected by production performance and meat production performance. This requires animal experiments to comprehensively consider multiple factors in order to come up with an application plan for low-protein diets. Summary of the invention
[0005] In order to solve the above technical problems, the present invention proposes a low-protein diet for broilers in the later growth stage and its application, which is a low-protein diet with balanced amino acids, which can improve the egg white utilization rate and production performance of broilers, reduce nitrogen emissions in broiler production, and reduce environmental pollution caused by broiler production.
[0006] To achieve the above object, the present invention provides a low-protein diet for broiler chickens in the later growth period, comprising the following components in parts by weight:
[0007] 53-62 parts of corn, 14-23 parts of soybean meal, 5 parts of corn gluten meal, 10 parts of wheat flour, 1.20-1.22 parts of stone powder, 3.46-4.58 parts of soybean oil, 1.30-1.43 parts of calcium hydrogen phosphate, 0.2-0.3 parts of salt, 0.20-0.25 parts of choline chloride, 0.2 parts of broiler trace elements, 0.23-0.30 parts of DL-methionine, 0.3-0.6 parts of L-lysine hydrochloride, 0.02 parts of broiler vitamins, L-phenyl 0.10-0.17 parts of alanine, 0.08-0.20 parts of L-threonine, 0.13-0.50 parts of L-arginine, 0.14-0.24 parts of L-isoleucine, 0.01-0.05 parts of L-tryptophan, 0.05-0.30 parts of L-valine, 0.05-0.15 parts of glycine, 0.02 parts of phytase, 0.10-0.55 parts of zeolite powder, 0.11-0.37 parts of potassium carbonate, and 0.09-0.18 parts of sodium bicarbonate.
[0008] Preferably, the low protein diet is used for broiler Ward 188.
[0009] Preferably, the low-protein diet is used for broiler chickens in the later growth period, specifically for broiler chickens aged 29 to 42 days.
[0010] Preferably, the broiler trace elements include one or more of Cu, Zn, Fe, Mn, Se and I.
[0011] Further preferably, the mass ratio of Cu:Zn:Fe:Mn:Se:I in the broiler trace elements is 8:75:80:100:0.15:0.35.
[0012] Preferably, the broiler vitamins include one or more of vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, pantothenic acid, niacin, folic acid and biotin.
[0013] Further preferably, the mass ratio of vitamin A: vitamin D3: vitamin E: vitamin K3: vitamin B1: vitamin B2: vitamin B6: vitamin B12: pantothenic acid: niacin: folic acid: biotin in the broiler vitamins is 4.5 mg: 0.9 mg: 0.00675 mg: 3 mg: 3 mg: 8 mg: 6 mg: 0.03 mg: 17.64 mg: 44 mg: 17.64 mg: 0.15 mg.
[0014] Preferably, the phytase is a thermostable phytase, and the enzyme activity of the phytase is ≥5000 U / g.
[0015] The invention also provides application of the low-protein diet in broiler breeding.
[0016] Preferably, the broiler is a Ward 188 broiler, and the low-protein diet is used to feed broilers of 29 to 42 days of age.
[0017] Compared with the prior art, the present invention has the following advantages and technical effects:
[0018] The present invention provides a low-protein diet with amino acid balance by determining the required amount and balance pattern of arginine, valine and isoleucine, which are limiting amino acids of broilers, to improve the utilization rate of egg white and production performance of broilers, reduce nitrogen emissions from broiler production, and reduce environmental pollution caused by broiler production. On the basis of meeting the nutritional requirements of broiler growth, the low-protein diet can effectively reduce the crude protein content and soybean meal dosage in the broiler diet, improve the protein utilization rate of broilers, and thus reduce the nitrogen emissions in broiler feces.
[0019] One of the technical principles of the present invention: low-protein diet technology is to adjust the nutritional components of feed by reducing the protein content in the formula. However, low-protein diets may lead to an imbalance of essential amino acids and non-essential amino acids. In low-protein diets, the intake of essential amino acids may be insufficient, affecting the growth performance of broilers. In order to make up for this deficiency, suitable essential amino acids, such as lysine, methionine and arginine, can be added to low-protein diets to maintain amino acid balance and ensure normal growth and production of broilers. Therefore, the application of low-protein diet technology will help adjust the protein content of new white-feathered fast-growing large broiler feed, avoid the problem of overnutrition, and maintain amino acid balance by adding essential amino acids and an appropriate amount of non-essential amino acids, thereby improving the growth performance of broilers, improving the protein utilization of broilers, reducing nitrogen emissions, and reducing environmental pollution.
[0020] The low-protein diet provided by the present invention can reduce the protein level of the diet while ensuring the balance of amino acids by reducing crude protein and adding additional amino acids, and the growth performance and slaughter performance of the low-protein diet group can reach a level comparable to that of a conventional diet, which can reduce the amount of protein raw materials and more reasonably utilize feed raw materials. The low-protein diet formula is suitable for feed processing plants and farms to prepare feed, and can be fed directly. In addition to nutrients, no other additives are added, which is safe and harmless to broiler production.
[0021] The present invention reduces the dietary protein level of fast-growing white-feathered broilers from 20% to 18% in the late fattening stage at 29 to 42 days of age, balances all essential amino acids such as lysine, protein, thiocyanate, amino acid, spermatocyanin, valine, isoleucine, etc., and can effectively reduce the crude protein content in the broiler diet on the basis of meeting the nutritional requirements of the broiler growth, improve the protein utilization rate of the broiler, thereby reducing the nitrogen content in the broiler feces and reducing the environmental pollution caused by broiler production.
[0022] The present invention uses the Box-Behnken design of response surface design to determine the requirements of arginine, valine and isoleucine for Ward 188 broilers and the optimal ratio among the three under low-protein diet conditions, and to explore the requirements and balance pattern of the three amino acids for Ward 188 broilers under low-protein diet. DETAILED DESCRIPTION
[0023] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.
[0024] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.
[0025] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.
[0026] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to the skilled artisan. The present invention description and examples are exemplary only.
[0027] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. If not otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available commodities. The broiler trace elements and broiler vitamins used in the following examples are all purchased from Beijing Zhongnong Youjia Biotechnology Co., Ltd. The enzyme activity of phytase (thermostable phytase) is 10000U / g, purchased from Beijing Zhongnong Youjia Biotechnology Co., Ltd.
[0028] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.
[0029] Unless otherwise specified, the "parts" described in the present invention are all based on mass parts.
[0030] Example 1
[0031] 61.06 parts of corn, 14.14 parts of soybean meal, 5 parts of corn gluten meal, 10 parts of wheat flour, 1.21 parts of stone powder, 3.47 parts of soybean oil, 1.43 parts of calcium hydrogen phosphate, 0.20 parts of salt, 0.25 parts of choline chloride, 0.2 parts of broiler trace elements, 0.30 parts of DL-methionine, 0.56 parts of L-lysine hydrochloride, 0.02 parts of broiler vitamins, 0.17 parts of L-phenylalanine, 0.20 parts of L-threonine, 0.36 parts of L-arginine, 0.16 parts of L-isoleucine, 0.05 parts of L-tryptophan, 0.19 parts of L-valine, 0.15 parts of glycine, 0.02 parts of phytase, 0.32 parts of zeolite powder, 0.37 parts of potassium carbonate, and 0.18 parts of sodium bicarbonate.
[0032] The mass ratio of Cu:Zn:Fe:Mn:Se:I in broiler trace elements is 8:75:80:100:0.15:0.35.
[0033] The mass ratios of vitamin A: vitamin D3: vitamin E: vitamin K3: vitamin B1: vitamin B2: vitamin B6: vitamin B12: pantothenic acid: niacin: folic acid: biotin in broiler vitamins are 4.5mg: 0.9mg: 0.00675mg: 3mg: 3mg: 8mg: 6mg: 0.03mg: 17.64mg: 44mg: 17.64mg: 0.15mg.
[0034] The phytase is a thermostable phytase, and the enzyme activity of the phytase is 10000 U / g.
[0035] Example 2
[0036] 53 parts of corn, 14 parts of soybean meal, 5 parts of corn gluten meal, 10 parts of wheat flour, 1.20 parts of stone powder, 3.46 parts of soybean oil, 1.30 parts of calcium hydrogen phosphate, 0.25 parts of salt, 0.20 parts of choline chloride, 0.2 parts of broiler trace elements, 0.23 parts of DL-methionine, 0.3 parts of L-lysine hydrochloride, 0.02 parts of broiler vitamins, 0.10 parts of L-phenylalanine, 0.08 parts of L-threonine, 0.13 parts of L-arginine, 0.14 parts of L-isoleucine, 0.01 parts of L-tryptophan, 0.05 parts of L-valine, 0.05 parts of glycine, 0.02 parts of phytase, 0.10 parts of zeolite powder, 0.11 parts of potassium carbonate, and 0.09 parts of sodium bicarbonate.
[0037] The mass ratio of Cu:Zn:Fe:Mn:Se:I in broiler trace elements is 8:75:80:100:0.15:0.35.
[0038] The mass ratios of vitamin A: vitamin D3: vitamin E: vitamin K3: vitamin B1: vitamin B2: vitamin B6: vitamin B12: pantothenic acid: niacin: folic acid: biotin in broiler vitamins are 4.5mg: 0.9mg: 0.00675mg: 3mg: 3mg: 8mg: 6mg: 0.03mg: 17.64mg: 44mg: 17.64mg: 0.15mg.
[0039] The phytase is a thermostable phytase, and the enzyme activity of the phytase is 10000 U / g.
[0040] Example 3
[0041] 62 parts of corn, 23 parts of soybean meal, 5 parts of corn gluten meal, 10 parts of wheat flour, 1.22 parts of stone powder, 4.58 parts of soybean oil, 1.40 parts of calcium hydrogen phosphate, 0.30 parts of salt, 0.23 parts of choline chloride, 0.2 parts of broiler trace elements, 0.25 parts of DL-methionine, 0.6 parts of L-lysine hydrochloride, 0.02 parts of broiler vitamins, 0.17 parts of L-phenylalanine, 0.010 parts of L-threonine, 0.50 parts of L-arginine, 0.24 parts of L-isoleucine, 0.05 parts of L-tryptophan, 0.19 parts of L-valine, 0.15 parts of glycine, 0.02 parts of phytase, 0.55 parts of zeolite powder, 0.25 parts of potassium carbonate, and 0.15 parts of sodium bicarbonate.
[0042] The mass ratio of Cu:Zn:Fe:Mn:Se:I in broiler trace elements is 8:75:80:100:0.15:0.35.
[0043] The mass ratios of vitamin A: vitamin D3: vitamin E: vitamin K3: vitamin B1: vitamin B2: vitamin B6: vitamin B12: pantothenic acid: niacin: folic acid: biotin in broiler vitamins are 4.5mg: 0.9mg: 0.00675mg: 3mg: 3mg: 8mg: 6mg: 0.03mg: 17.64mg: 44mg: 17.64mg: 0.15mg.
[0044] The phytase is a thermostable phytase, and the enzyme activity of the phytase is 10000 U / g.
[0045] Experimental Example 1
[0046] The 3-factor, 3-level Box-Behnken response surface design method was adopted, i.e., three levels of arginine, valine and isoleucine. A total of 1680 healthy Ward 188 commercial broilers of similar weight were selected for the experiment, half of which were male and half were female. The experiment was divided into three stages (early stage: 1-14d; mid-stage: 15-28d; late stage: 29-42d). The basal broiler diet was fed in the early and mid-stages. In the late stage, 840 broilers of both sexes were randomly divided into 14 treatments according to gender, with 6 replicates in each treatment and 10 chickens in each replicate. One control group and 13 low-protein diet groups were set up (according to the Box-Behnken design). The control group broilers were fed with a conventional basal diet, and the experimental group was fed with a low-protein diet (the protein in the basal diet was reduced by 2%). The specific experimental design is shown in Table 2.
[0047] Table 1 Dietary composition
[0048]
[0049]
[0050] The diet formula is formulated with reference to the NY-T33-2004 chicken feeding standard, with a scientific ratio of amino acids. While reducing the protein content, it focuses on the ratio of essential amino acids to non-essential amino acids. The scientific ratio of amino acids helps to improve protein utilization, reduce nitrogen emissions, and reduce environmental pollution.
[0051] The preparation method of low-protein diet is as follows: quantitatively weigh each component in proportion, first evenly mix broiler trace elements, choline chloride, potassium carbonate, calcium hydrogen phosphate, sodium bicarbonate, L-lysine hydrochloride, DL-methionine, L-threonine, L-tryptophan, L-arginine, L-isoleucine, L-valine, L-phenylalanine, glycine, zeolite powder, broiler vitamins, and phytase into small materials; then evenly mix the mixed small materials with other raw materials, and finally steam granulate the mixed feed. This formula has a scientific proportion and a simple production process. Essential amino acids are added to the white-feathered broiler feed formula, reducing the protein level of the fast-growing white-feathered broiler formula from 20% to 18% in the later stage, and ensuring the nutritional needs of white-feathered broilers. The entire experimental design is shown in Table 2.
[0052] Table 2 Experimental group design
[0053]
[0054]
[0055] The present invention uses the growth curve as the basis for division, and divides the age from 29 to 42 days into the late growth period, during which the diet is fed in a free-feeding manner.
[0056] After 42 days of age, the following analyses were performed on the broilers in the low-protein diet group:
[0057] 1. The results of univariate analysis of growth performance of broiler chickens aged 29 to 42 days are shown in Table 3. The growth performance indicators in Table 3 were plotted as a response surface, and the model P value is shown in Table 4. For hens, SID.Arg, SID.Val, and SID.Ile had significant effects on their body weight and body weight gain (P < 0.05), but had no significant effect on their feed intake and feed-to-weight ratio (P > 0.05); for roosters, SID.Arg, SID.Val, and SID.Ile had significant effects on their body weight, body weight gain, and feed intake (P < 0.05), but had no significant effect on their feed-to-weight ratio (P > 0.05); the combination that achieved the best growth performance is shown in Table 5. Taking into account the growth performance indicators of hens and roosters, for hens, the best growth performance was achieved when the SID.Arg / Lys level was 120.06%, the SID.Val / Lys level was 69%, and the SID.Ile / Lys level was 77%; for roosters, the best growth performance was achieved when the SID.Arg / Lys level was 122%, the SID.Val / Lys level was 82.37%, and the SID.Ile / Lys level was 77%.
[0058] Table 3 Single factor ANOVA analysis of growth performance of broiler chickens aged 29 to 42 days
[0059]
[0060]
[0061] Table 4 P value of response surface model for growth performance of broiler chickens aged 9 to 42 days
[0062]
[0063]
[0064]
[0065] Note: “-” indicates that the indicator model is a linear model without interaction and quadratic effect.
[0066] Table 5 Response surface analysis results of growth performance of broiler chickens aged 29 to 42 days
[0067]
[0068] 2. The results of univariate analysis of slaughter performance of 42-day-old broilers are shown in Table 6. The slaughter performance indicators in Table 6 are plotted as a response surface, and the model P value is shown in Table 7. For hens, SID.Arg, SID.Val, and SID.Ile have significant effects on their slaughter rate, full carcass rate, half-carcass rate, abdominal fat rate, and breast muscle rate (P < 0.05), but have no significant effect on their leg muscle rate (P > 0.05); for roosters, SID.Arg, SID.Val, and SID.Ile have significant effects on their body slaughter rate, full carcass rate, half-carcass rate, and breast muscle rate (P < 0.05), but have no significant effects on their abdominal fat rate and leg muscle rate (P > 0.05); the combination for achieving the best slaughter performance is shown in Table 8. Taking into account the slaughter performance indicators of hens and roosters, for hens, the slaughter performance is best when the SID.Arg / Lys level is 109.70%, the SID.Val / Lys level is 74.80%, and the SID.Ile / Lys level is 61%; for roosters, the slaughter performance is best when the SID.Arg / Lys level is 110.88%, the SID.Val / Lys level is 79.96%, and the SID.Ile / Lys level is 69.28%.
[0069] Table 64 Single factor variance analysis of slaughter performance of 2-day-old broilers
[0070]
[0071]
[0072] Table 74 P value of response surface model for slaughter performance of 2-day-old broiler chickens
[0073]
[0074]
[0075] Table 842 Days old broiler slaughter performance response surface analysis results
[0076]
[0077] 3. The results of fecal nitrogen content of low-protein diets are shown in Table 9. Compared with the control example, the fecal nitrogen content of Example 1 was significantly reduced (P < 0.01). For hens, the fecal nitrogen content of the low-protein group was higher than that of the control example. This was due to the reduced utilization of broiler egg white caused by amino acid imbalance, so its nitrogen emission increased, indicating that amino acid imbalance and low-protein diets would also lead to increased nitrogen emissions in broilers; for roosters, the fecal nitrogen content of the low-protein diet was significantly reduced.
[0078] Table 9 Nitrogen content in broiler feces from 29 to 42 days old
[0079]
[0080] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.
Claims
1. A low-protein diet for broiler chickens in the late growth period, characterized in that: It comprises the following components in parts by weight: 53-62 parts of corn, 14-23 parts of soybean meal, 5 parts of corn gluten meal, 10 parts of wheat flour, 1.20-1.22 parts of stone powder, 3.46-4.58 parts of soybean oil, 1.30-1.43 parts of calcium hydrogen phosphate, 0.2-0.3 parts of salt, 0.20-0.25 parts of choline chloride, 0.2 parts of broiler trace elements, 0.23-0.30 parts of DL-methionine, 0.3-0.6 parts of L-lysine hydrochloride, 0.02 parts of broiler vitamins, L-phenyl 0.10-0.17 parts of alanine, 0.08-0.20 parts of L-threonine, 0.13-0.50 parts of L-arginine, 0.14-0.24 parts of L-isoleucine, 0.01-0.05 parts of L-tryptophan, 0.05-0.30 parts of L-valine, 0.05-0.15 parts of glycine, 0.02 parts of phytase, 0.10-0.55 parts of zeolite powder, 0.11-0.37 parts of potassium carbonate, and 0.09-0.18 parts of sodium bicarbonate.
2. The low-protein diet according to claim 1, characterized in that The low protein diet was used for broiler Ward 188 chickens.
3. The low-protein diet according to claim 1, characterized in that The low-protein diet is used for broiler chickens in the later growth period, specifically for broiler chickens aged 29 to 42 days.
4. The low-protein diet according to claim 1, characterized in that The broiler trace elements include one or more of Cu, Zn, Fe, Mn, Se and I.
5. The low-protein diet according to claim 4, characterized in that The mass ratio of Cu:Zn:Fe:Mn:Se:I in the broiler trace elements is 8:75:80:100:0.15:0.
35.
6. The low-protein diet according to claim 1, characterized in that The broiler vitamins include one or more of vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, pantothenic acid, niacin, folic acid and biotin.
7. The low-protein diet according to claim 6, characterized in that The mass ratio of vitamin A: vitamin D3: vitamin E: vitamin K3: vitamin B1: vitamin B2: vitamin B6: vitamin B12: pantothenic acid: niacin: folic acid: biotin in the broiler vitamins is 4.5mg: 0.9mg: 0.00675mg: 3mg: 3mg: 8mg: 6mg: 0.03mg: 17.64mg: 44mg: 17.64mg: 0.15mg.
8. The low-protein diet according to claim 1, characterized in that The phytase is a thermostable phytase, and the enzyme activity of the phytase is ≥5000 U / g.
9. Use of the low-protein diet according to any one of claims 1 to 8 in broiler farming.
10. The use according to claim 9, characterized in that: The broilers are Ward 188 broilers, and the low-protein diet is used to feed broilers of 29 to 42 days old.