Screen-strengthening repairing component as well as preparation method and application thereof

Abstract

The invention discloses a screen-strengthening repairing component as well as a preparation method and application thereof, and relates to the technical field of daily necessities. The screen strengthening and repairing component is prepared from the following components in parts by weight: 0.001 to 20 parts of glycosyl glycosides extracted from desert algae, 0.001 to 10 parts of acetyl hydroxyproline and 0.001 to 20 parts of panthenol. According to the screen strengthening and repairing component provided by the invention, through the synergistic effect of the glycosylglycerol, the acetyl hydroxyproline and the panthenol, efficient screen repairing is provided from multiple aspects of moisturizing, lipid repairing, anti-inflammatory relieving and the like. The combination of the three components complement each other in barrier repair to form a set of comprehensive barrier repair scheme which is suitable for skin care requirements of damaged, sensitive and dry barriers. The screen-strengthening repairing component disclosed by the invention not only can meet basic requirements of screen repairing, but also can provide multi-effect and lasting screen protection, and can be applied to various cosmetics and skin care products.

Description

Technical Field

[0001] The present invention relates to the technical field of daily necessities, and in particular to a strong screen repairing component and a preparation method and application thereof. Background Art

[0002] The skin barrier is the outermost protective barrier of the skin, which can effectively prevent external harmful substances (such as pollutants, bacteria, ultraviolet rays, etc.) from entering the skin and prevent the loss of water from the skin. A healthy skin barrier is essential for maintaining skin moisture, anti-aging, reducing skin sensitivity and inflammatory response. However, due to the influence of multiple factors such as environmental factors, skin diseases, aging, and lifestyle habits, the skin barrier is easily damaged, leading to problems such as dry, rough, sensitive, and susceptible to infection.

[0003] Most skin barrier repair products on the market currently have the following problems: single efficacy, lack of comprehensive repair effects;

[0004] The ingredients in the formula fail to fully exert their synergistic effects; the repair effect is poorly sustained, making it difficult to maintain the long-term health of the skin barrier.

[0005] Therefore, developing a composition that can comprehensively repair the skin barrier, improve skin tolerance and have a lasting effect is a technical problem that needs to be solved urgently. Therefore, how to supplement and generate collagen through a more effective and safer way has become a key technical problem in the field of skin care.

[0006] In view of this, the present invention is proposed. Summary of the invention

[0007] The purpose of the present invention is to provide a strong screen repairing component and a preparation method and application thereof.

[0008] The present invention is achieved in that:

[0009] In a first aspect, the present invention provides a strong screen repairing ingredient, the components of which include, by weight, 0.001-20 parts of glycerol glucoside extracted from desert algae, 0.001-10 parts of acetylhydroxyproline and 0.001-20 parts of panthenol.

[0010] In an optional embodiment, the components include, by mass percentage, 0.01-10 parts of glycerol glucoside extracted from desert algae, 0.01-8 parts of acetylhydroxyproline, and 0.01-10 parts of panthenol;

[0011] Preferably, the components include, by mass percentage, 0.1-5 parts of glycerol glucoside extracted from desert algae, 0.05-5 parts of acetylhydroxyproline and 0.1-5 parts of panthenol.

[0012] In a second aspect, the present invention provides a method for preparing a strong screen repairing ingredient, which comprises uniformly mixing the components of the strong screen repairing ingredient described in any one of the above embodiments, wherein the method for preparing the glycerol glucoside extracted from desert algae comprises:

[0013] The powder of desert algae is mixed with an ethanol solution, and ultrasonic extraction is performed at a temperature of 30° C. to 40° C. for 20 to 40 minutes, and the extraction is repeated 2 to 4 times, and the extracts are combined and centrifuged, and the supernatant is concentrated under reduced pressure to 15 to 25% of the original volume to obtain a concentrated solution, and the concentrated solution is separated by C18 reverse phase silica gel column chromatography, and gradient elution is performed using water and ethanol, and the eluate containing glycerol glucoside is collected;

[0014] The eluate is placed in an ultrafiltration membrane system with a molecular weight cutoff value of 3000Da for filtration to obtain a filtrate, and the filtrate is cooled to 3-5°C and allowed to stand to induce crystallization of glycerol glucoside to obtain glycerol glucoside powder with a purity of ≥95%.

[0015] In an optional embodiment, the desert algae include at least one of Chlorella vulgaris, Microcoleus scaly, Hasa algae, and Cyanobacteria;

[0016] Preferably, the mass ratio of the desert algae powder to the ethanol solution is 1:8-12, and the concentration of the ethanol solution is 70-80%;

[0017] Preferably, the centrifugal stirring speed is 3000-10000 rpm, and the centrifugal time is 8-15 min.

[0018] In an optional embodiment, the gradient elution comprises:

[0019] (1) Loading: Slowly add the dissolved concentrate to a C18 reverse phase silica gel column, use ethanol as the eluent to press the sample onto the silica gel, and adjust the flow rate and mobile phase.

[0020] (2) Elution: Use pure water, 30% ethanol, 50% ethanol, 70% ethanol and 100% ethanol in turn for elution in separate tubes or bottles;

[0021] (3) Treatment of the eluate: Take a portion of the eluate from each tube or bottle and place it in a small test tube. Place it in a centrifugal concentrator for concentration and then add 0.5 mL of ethanol to dissolve it again. Then perform thin layer chromatography analysis. Combine the components with the same Rf value and consistent color development and collect the eluate containing glycerol glucoside.

[0022] In a third aspect, the present invention provides an application of a strong screen repairing ingredient as described in any one of the above embodiments or a strong screen repairing ingredient prepared by the preparation method of the strong screen repairing ingredient as described in any one of the above embodiments in the preparation of cosmetics.

[0023] In a fourth aspect, the present invention provides a strong screen repairing cosmetic, comprising the strong screen repairing ingredient as described in any one of the above embodiments, and based on the total mass of the strong screen repairing cosmetic as 100%, the content of the strong screen repairing ingredient is 0.003-50%.

[0024] In an optional embodiment, the screen repair cosmetic further comprises at least one of an emulsifier, an antioxidant, an emollient, a thickener, a moisturizer, a chelating agent, a flavoring agent, a preservative and a pH adjuster and the balance is water;

[0025] Preferably, based on the total mass of the strong screen repair cosmetics as 100%, the content of the emulsifier is 0-20%, the content of the antioxidant is 0-10%, the content of the emollient is 0-50%, the content of the thickener is 0.01%-8%, the content of the moisturizer is 0.01%-40%, the content of the chelating agent is 0.01%-1%, the content of the flavoring agent is 0-1%, the content of the preservative is 0.01-3%, and the content of the pH adjuster is 0-3%.

[0026] In an optional embodiment, the emulsifier is selected from one or more of PEG-100 stearate, polyglyceryl-3 methyl glucose distearate, potassium cetyl phosphate, cetearyl glucoside, cetearyl olive oil ester, sodium methyl stearoyl taurate, sodium methyl stearoyl taurate, hydrogenated lecithin, sorbitan olive oil ester and polyglyceryl-3 distearate;

[0027] And / or, the antioxidant is selected from one or more of vitamin E and its derivatives, vitamin C and its derivatives, ubiquinone and fullerene;

[0028] And / or, the emollient is selected from one or more of tri(ethylhexanoin)glyceryl, caprylic / capric triglyceride, pentaerythritol tetra(ethylhexanoin), hydrogenated polyisobutene, butyloctanol salicylate, diethoxyethyl succinate, isododecane, rice germ oil, hydrogenated palm kernel oil, cocoa seed butter cetyl ethylhexanoate, isopropyl myristate, isopropyl palmitate, diisopropyl sebacate, polydimethylsiloxane, cetearyl alcohol, dibutyl adipate, C12-15 alcohol benzoate, dicaprylyl carbonate, coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, caprylyl polymethylsiloxane, cyclopentasiloxane and phenyl trimethicone;

[0029] And / or, the thickener is selected from one or more of xanthan gum, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, carbomer, ammonium acryloyldimethyltaurate / VP copolymer and sodium acrylate / sodium acryloyldimethyltaurate copolymer;

[0030] And / or, the humectant is selected from one or more of glycerol, 1,3-propylene glycol, butylene glycol, dipropylene glycol, pentylene glycol, sorbitol, erythritol, 1,2-hexanediol, glyceryl polyether-26 and caprylyl glycol;

[0031] And / or, the chelating agent is selected from one or a combination of two or more of disodium EDTA, tetrasodium EDTA and sodium phytate;

[0032] And / or, the flavoring agent is selected from one or both of essence and essential oil;

[0033] and / or, the preservative is selected from one or more of phenoxyethanol, p-hydroxyacetophenone, sodium benzoate, phenoxyethanol, methylparaben, caprylhydroxamic acid and chlorphenesin;

[0034] And / or, the pH regulator is selected from one or more of arginine, sodium hydroxide, potassium hydroxide, triethanolamine and aminomethyl propanol.

[0035] In an optional embodiment, the screen-repairing cosmetic is selected from any one of skin care water, essence, lotion, face cream, face mask, sunscreen, BB cream and isolation cream.

[0036] In a fifth aspect, the present invention provides a method for preparing the strong screen repairing cosmetic as described in any one of the aforementioned embodiments, comprising the following steps:

[0037] S1: Stir and heat water, thickener, humectant, chelating agent, emollient and emulsifier to 80-90°C in sequence and mix well;

[0038] S2: Add pH adjuster and mix well;

[0039] S3: Cool down to 40-50℃, add preservatives and strong screen repair ingredients, mix evenly, and discharge.

[0040] The present invention has the following beneficial effects:

[0041] The strong screen repairing ingredient provided by the present invention provides efficient barrier repair from multiple aspects such as moisturizing, lipid repair and anti-inflammatory soothing through the synergistic effects of glycerol glucoside, acetylhydroxyproline and panthenol. The combination of these three ingredients complements each other in barrier repair, forming a comprehensive barrier repair solution that is suitable for skin care needs for damaged, sensitive and dry skin. The strong screen repairing ingredient of the present invention can not only meet the basic needs of barrier repair, but also provide multi-effect and long-lasting barrier protection. The strong screen repairing ingredient provided by the present invention can be applied to various cosmetics and skin care products. DETAILED DESCRIPTION

[0042] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0043] The invention provides a strong screen repairing component, the components of which include 0.001-20 parts of glycerol glucoside extracted from desert algae, 0.001-10 parts of acetylhydroxyproline and 0.001-20 parts of panthenol by weight.

[0044] The present invention provides efficient barrier repair from multiple aspects such as moisturizing, lipid repair, and anti-inflammatory relief through the synergistic effects of glyceryl glucoside, acetyl hydroxyproline, and panthenol. The functions of the effective components of the strong barrier repair ingredients provided by the present invention are introduced as follows:

[0045] Glyceryl Glucoside can promote hydration and moisturizing.

[0046] (1) Aquaporin activation: Glyceryl glucoside is a powerful moisturizing ingredient that can activate the synthesis of aquaporin (AQP3). Aquaporins are water channels on the skin cell membrane that promote the flow and transfer of water molecules between cells, effectively improving the hydration of the stratum corneum.

[0047] (2) Long-lasting moisturizing: The sugar molecules in the glyceryl glucoside structure can absorb water and moisturize for a long time, ensuring that the skin remains hydrated. This provides a more ideal skin environment for other repair ingredients and helps to repair the overall barrier.

[0048] Acetyl Hydroxyproline can play a role in lipid repair and anti-glycation.

[0049] (1) Promote ceramide synthesis: Acetylhydroxyproline is a precursor for ceramide synthesis and can effectively promote the production of ceramides such as EOP (Ceramide 1), NS (Ceramide 2) and AP (Ceramide 3) in the skin. These ceramides are the main components of the lipid layer of the skin barrier and play a key role in repairing the lipid structure of the barrier and restoring the "brick wall structure" of the skin.

[0050] (2) Anti-glycation effect: Acetylhydroxyproline also has anti-glycation ability, which can prevent sugar molecules from reacting with proteins in the skin (such as collagen) to form glycation reactions, reduce hardening and damage to the skin, delay the skin aging process, and improve the health of the skin barrier.

[0051] Panthenol has a soothing and anti-inflammatory effect.

[0052] (1) Repair and relieve inflammation: Panthenol (a precursor of vitamin B5) has significant soothing and anti-inflammatory effects, and can quickly relieve skin discomfort such as redness and stinging. It can inhibit the release of inflammatory factors, reduce inflammatory responses, and is suitable for repairing sensitive skin and damaged skin.

[0053] (2) Promote cell proliferation and regeneration: Panthenol can promote the proliferation of stratum corneum cells and the regeneration of skin tissue, which helps the skin to repair itself. In addition, it has a certain moisturizing effect, further improving the skin's hydration state.

[0054] The synergistic mechanism of glyceryl glucoside, acetyl hydroxyproline and panthenol:

[0055] (1) Strengthening the barrier structure: The moisturizing effect of glyceryl glucoside provides a good moisture environment for the skin, making the synthesis of ceramide promoted by acetylhydroxyproline more efficient, thereby repairing and consolidating the lipid structure of the barrier.

[0056] (2) Multiple repairs and all-round protection: The lipid repair and anti-glycation effects of acetyl hydroxyproline, the soothing and anti-inflammatory effects of panthenol, and the moisturizing effects of glyceryl glucoside work together to form an all-round barrier repair from the inside out, from cells to structures, making the skin healthier and more stable.

[0057] (3) Long-lasting hydration and improved tolerance: Through the long-lasting moisturizing of glyceryl glucoside, the ceramide support of acetyl hydroxyproline, and the soothing effect of panthenol, the skin's hydration and tolerance are significantly improved, and the skin can better cope with the stimulation and damage of the external environment.

[0058] The combination of these three ingredients complement each other in barrier repair, forming a comprehensive barrier repair solution that is suitable for the care needs of damaged, sensitive and dry skin. The strong barrier repair ingredients of the present invention can not only meet the basic needs of barrier repair, but also provide multi-effect and long-lasting barrier protection.

[0059] In some preferred embodiments, the components include, by mass percentage, 0.01-10 parts of glycerol glucoside extracted from desert algae, 0.01-8 parts of acetylhydroxyproline, and 0.01-10 parts of panthenol; further preferably, the components include, by mass percentage, 0.1-5 parts of glycerol glucoside extracted from desert algae, 0.05-5 parts of acetylhydroxyproline, and 0.1-5 parts of panthenol.

[0060] In addition, the preparation method of the strong screen repairing component provided by the present invention is simple, and the components of the strong screen repairing component on the market are mixed evenly, wherein the preparation method of the glycerol glucoside extracted from desert algae comprises:

[0061] S1. Mix the powder of desert algae with 70-80% ethanol solution in a mass ratio of 1:8-12, perform ultrasonic extraction at a temperature of 30°C-40°C for 20-40 minutes, repeat the extraction 2-4 times, and replace with fresh ethanol solution each time to ensure complete extraction of glycerol glucoside.

[0062] S2. Combine the extracts and centrifuge at a stirring speed of 3000-10000 rpm for 8-15 min to remove insoluble impurities and obtain a clear supernatant.

[0063] S3. The supernatant is concentrated under reduced pressure to 15-25% of the original volume to obtain a concentrated solution. The concentrated solution can remove the ethanol in the supernatant and retain the active ingredient of glycerol glucoside.

[0064] S4. Separate the concentrate by C18 reverse phase silica gel column chromatography, using water and ethanol as gradient elution, and collect the eluate containing glycerol glucoside.

[0065] S5. The eluate is placed in an ultrafiltration membrane system with a molecular weight cutoff value of 3000Da for filtration to obtain a filtrate, and the filtrate is cooled to 3-5°C for standing to induce crystallization of glycerol glucoside to obtain glycerol glucoside powder with a purity of ≥95%. The purity detection method is to detect the purity of glycerol glucoside by high performance liquid chromatography to ensure that it reaches more than 95%. The dried glycerol glucoside powder is sealed and stored in a low temperature dry environment to maintain its activity and stability.

[0066] Wherein, the powder of desert algae is obtained by grinding the desert algae after freezing. The operation of grinding after freezing can break the cell wall and release the cell contents, which is convenient for subsequent extraction. In the present invention, desert algae includes but is not limited to at least one of Chlorella, Microcoa algae with sheath, Hasa algae and cyanobacteria. Wherein, Chlorella, Microcoa algae with sheath, Hasa algae and cyanobacteria can all be purchased from conventional raw material manufacturers. The extraction methods of Chlorella, Microcoa algae with sheath, Hasa algae and cyanobacteria are the same, and the extraction effects are similar.

[0067] In the present invention, gradient elution comprises:

[0068] (1) Loading: Open the top of the chromatography column and use a clean rubber-tipped pipette to suck out the excess pure water. Use a new clean rubber-tipped pipette to slowly add the dissolved concentrate to the C18 reverse-phase silica gel column. Use ethanol as the eluent to press the sample onto the silica gel and adjust the flow rate and mobile phase.

[0069] (2) Elution: Use pure water, 30% ethanol, 50% ethanol, 70% ethanol and 100% ethanol in turn for elution in separate tubes or bottles;

[0070] (3) Treatment of the eluate: Take a portion of the eluate from each tube or bottle and place it in a small test tube. Place it in a centrifugal concentrator for concentration and then add 0.5 mL of ethanol to re-dissolve it. Then perform thin layer chromatography analysis. Combine the components with the same Rf value and consistent color development and collect the eluate containing glycerol glucoside.

[0071] The screen repairing component provided by the present invention can be applied to various cosmetics and skin care products. Specifically, the present invention provides a typical but non-limiting example: screen repairing cosmetics. The screen repairing cosmetics include the above screen repairing component, and the content of the screen repairing component is 0.003-50% based on the total mass of the screen repairing cosmetics as 100%.

[0072] Furthermore, the components of the screen repair cosmetics may also include at least one of an emulsifier, an antioxidant, an emollient, a thickener, a humectant, a chelating agent, a flavoring agent, a preservative and a pH adjuster.

[0073] Taking the total mass of the strong screen repair cosmetics as 100%, the content of emulsifier is 0-20%, the content of antioxidant is 0-10%, the content of emollient is 0-50%, the content of thickener is 0.01%-8%, the content of humectant is 0.01%-40%, the content of chelating agent is 0.01%-1%, the content of flavoring agent is 0-1%, the content of preservative is 0.01-3%, the content of pH adjuster is 0-3%, and the balance is water.

[0074] Preferably, based on the total mass of the strong screen repair cosmetics as 100%, the content of the emulsifier is 0-10%, the content of the antioxidant is 0-5%, the content of the emollient is 0-20%, the content of the thickener is 0.01%-2%, the content of the humectant is 0.01%-20%, the content of the chelating agent is 0.01%-0.1%, the content of the flavoring agent is 0-0.5%, the content of the preservative is 0.01-2%, and the content of the pH adjuster is 0-2%.

[0075] Further, the emulsifier is selected from but not limited to one or more of PEG-100 stearate, polyglyceryl-3 methyl glucose distearate, potassium cetyl phosphate, cetearyl glucoside, cetearyl olivetate, sodium methyl stearoyl taurate, sodium methyl stearoyl taurate, hydrogenated lecithin, sorbitan olivetate and polyglyceryl-3 distearate;

[0076] and / or, the antioxidant is selected from but not limited to one or more of vitamin E and its derivatives, vitamin C and its derivatives, ubiquinone and fullerene;

[0077] And / or, the emollient is selected from, but not limited to, tri(ethylhexanoin)glyceryl, caprylic / capric triglyceride, pentaerythritol tetra(ethylhexanoin), hydrogenated polyisobutene, butyloctyl salicylate, diethoxyethyl succinate, isododecane, rice germ oil, hydrogenated palm kernel oil, cocoa seed butter cetyl ethylhexanoate, isopropyl myristate, isopropyl palmitate, diisopropyl sebacate, polydimethylsiloxane, cetearyl alcohol, dibutyl adipate, C12-15 alcohol benzoate, dicaprylyl carbonate, coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, caprylyl polymethylsiloxane and one or more of cyclopentasiloxane and phenyl trimethicone;

[0078] and / or, the thickener is selected from but not limited to one or more of xanthan gum, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, carbomer, ammonium acryloyldimethyltaurate / VP copolymer and sodium acrylate / sodium acryloyldimethyltaurate copolymer;

[0079] and / or, the humectant is selected from but not limited to one or more of glycerin, 1,3-propylene glycol, butylene glycol, dipropylene glycol, pentylene glycol, sorbitol, erythritol, 1,2-hexanediol, glyceryl polyether-26 and caprylyl glycol;

[0080] and / or, the chelating agent is selected from but not limited to one or a combination of two or more of disodium EDTA, tetrasodium EDTA and sodium phytate;

[0081] And / or, the flavoring agent is selected from but not limited to one or two of essence and essential oil;

[0082] and / or, the preservative is selected from but not limited to one or more of phenoxyethanol, p-hydroxyacetophenone, sodium benzoate, phenoxyethanol, methylparaben, caprylhydroxamic acid and chlorphenesin;

[0083] And / or, the pH adjuster is selected from but not limited to one or more of arginine, sodium hydroxide, potassium hydroxide, triethanolamine and aminomethyl propanol.

[0084] The screen-repairing cosmetics provided by the present invention include, but are not limited to, any one of skin care lotion, essence, emulsion, face cream, face mask, sunscreen, BB cream and isolation cream.

[0085] In addition, the present invention provides a method for preparing a strong screen repairing cosmetic, comprising the following steps:

[0086] S1: Stir and heat water, thickener, humectant, chelating agent, emollient and emulsifier to 80-90°C in sequence and mix well;

[0087] S2: Add pH adjuster and mix well;

[0088] S3: Cool down to 40-50℃, add preservatives and strong screen repair ingredients, mix evenly, and discharge.

[0089] When the components contain an antioxidant, the antioxidant is also added in step S1.

[0090] The features and properties of the present invention are further described in detail below in conjunction with the examples. In the examples and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used can be purchased from conventional raw material manufacturers unless otherwise specified.

[0091] Example 1

[0092] This embodiment provides a strong screen repairing ingredient, which includes 0.1 parts of glyceryl glucoside, 0.05 parts of acetyl hydroxyproline and 0.1 parts of panthenol.

[0093] Wherein, the preparation method of glycerol glucoside comprises:

[0094] Chlorella (commercially available from Codif International) was frozen and ground to break the cell wall and release the cell contents to obtain desert algae powder, which was mixed with an ethanol (70%-80%) solution at a mass ratio of 1:10. The powder was placed in an ultrasonic-assisted extraction device and ultrasonically extracted at a temperature of 30°C-40°C for 30 minutes to improve the extraction efficiency. The extraction was repeated 3 times, and fresh ethanol solution was replaced each time to ensure that the glycerol glucoside was completely extracted. After filtering, the extract was placed in a centrifuge and centrifuged at a speed of 3000-10000rpm for 10 minutes to remove insoluble impurities and obtain a clear extract. The extract was placed in a reduced pressure evaporation device and concentrated at a temperature below 40°C until the volume of the solution was reduced to about 1 / 5 of the original volume to obtain a concentrated solution. This step helps to remove ethanol and retain the active ingredients of glycerol glucoside. The concentrate was separated by C18 reverse phase silica gel column chromatography, using water and ethanol gradient elution to collect the eluate containing glycerol glucoside. The eluate was filtered through an ultrafiltration membrane system with a molecular weight cutoff of 3000Da to remove small molecular impurities. The purified glycerol glucoside solution was cooled to 4°C and allowed to stand to induce crystallization of glycerol glucoside to obtain high-purity glycerol glucoside powder. The purity of glycerol glucoside was analyzed by high performance liquid chromatography (HPLC) to ensure that it reached more than 95%.

[0095] Example 2

[0096] This embodiment provides a strong screen repairing ingredient, which includes 0.5 parts of glyceryl glucoside, 0.1 parts of acetyl hydroxyproline and 0.5 parts of panthenol.

[0097] Example 3

[0098] This embodiment provides a strong screen repairing ingredient, which includes 1 part of glyceryl glucoside, 1 part of acetyl hydroxyproline and 1 part of panthenol.

[0099] Example 4

[0100] This embodiment provides a strong screen repairing ingredient, which includes 2 parts of glyceryl glucoside, 1.5 parts of acetyl hydroxyproline and 2 parts of panthenol.

[0101] Example 5

[0102] This embodiment provides a strong screen repairing ingredient, which includes 3 parts of glyceryl glucoside, 2 parts of acetyl hydroxyproline and 3 parts of panthenol.

[0103] Example 6

[0104] This embodiment provides a strong screen repairing ingredient, which includes 5 parts of glyceryl glucoside, 3 parts of acetyl hydroxyproline and 5 parts of panthenol.

[0105] Example 7

[0106] This embodiment provides a strong screen repairing ingredient, which includes 10 parts of glyceryl glucoside, 5 parts of acetyl hydroxyproline and 10 parts of panthenol.

[0107] Example 8

[0108] This embodiment provides a strong screen repairing ingredient, which includes 20 parts of glyceryl glucoside, 10 parts of acetyl hydroxyproline and 20 parts of panthenol.

[0109] Comparative Example 1

[0110] This comparative example provides a component comprising 0.5 parts of glyceryl glucoside.

[0111] Comparative Example 2

[0112] This comparative example provides a composition comprising 0.1 parts of acetylhydroxyproline.

[0113] Comparative Example 3

[0114] This comparative example provides a composition including 0.5 parts of panthenol.

[0115] Comparative Example 4

[0116] This comparative example provides a composition comprising 0.5 parts of glyceryl glucoside and 0.1 parts of acetylhydroxyproline.

[0117] Comparative Example 5

[0118] This comparative example provides a composition comprising 0.5 parts of glyceryl glucoside and 0.5 parts of panthenol.

[0119] Comparative Example 6

[0120] This comparative example provides a composition comprising 0.1 parts of acetyl hydroxyproline and 0.5 parts of panthenol.

[0121] Comparative Example 7

[0122] This comparative example provides a composition comprising 1 part of betaine.

[0123] Comparative Example 8

[0124] This comparative example provides a glyceryl glucoside prepared by another synthesis method. The preparation method is as follows:

[0125] (1) Preparation method: high temperature chemical catalysis method

[0126] (2) Reaction materials: glucose and glycerol

[0127] (3) Catalyst: strong acid catalyst (sulfuric acid)

[0128] (4) Reaction conditions

[0129] Mix glucose and glycerol in a molar ratio of 1:2. Add 0.1-1% acid catalyst (mass fraction). Stir the reaction at high temperature (above 150°C) for 4-6 hours. After the reaction, cool the reaction mixture to room temperature. Use a neutralizer (sodium carbonate) to neutralize the residual acid catalyst. Remove excess glycerol by distillation or vacuum drying. Use column chromatography to separate and obtain crude glycerol glucoside.

[0130] (5) Purification: The crude product is dissolved in ethanol and recrystallized to obtain relatively pure glycerol glucoside.

[0131] Comparative Example 9

[0132] Glyceryl glucoside is extracted from Osmanthus fragrans, and glyceryl glucoside powder is obtained according to the extraction method of desert algae.

[0133] Experimental example

[0134] This experimental example provides a strong screen repair cosmetic (calculated as 100 parts), the formula of which includes:

[0135] Phase A: 0.05 parts of chelating agent, 5 parts of moisturizing agent, 5 parts of emollient, 0.5 parts of emulsifier, 0.8 parts of thickener and the balance of deionized water.

[0136] Phase B: the ingredients of Examples 1-8 and Comparative Examples 1-10 and 0.5 parts of preservatives.

[0137] Among them, the chelating agent is EDTA-2Na, the humectant is glycerin, the emollient is caprylic / capric triglyceride, the emulsifier is hydrogenated lecithin, the thickener is polyacrylate crosspolymer-6, and the preservative is phenoxyethanol.

[0138] The specific preparation method is as follows:

[0139] S1. Weigh each component according to the formula;

[0140] S2. Dissolve the components of phase A at 85°C and stir until there are no particles;

[0141] S3. After cooling to below 45° C., add the phase B component and stir evenly to prepare a strong screen repairing cosmetic.

[0142] Examples of the present invention are shown in Table 1:

[0143] Table 1. Component statistics of different cosmetic examples

[0144]

[0145]

[0146] Efficacy test 1: Analysis of transepidermal water loss rate

[0147] Trans-epidermal water loss (TEWL) is measured by Tewameter, TM300, and is an important parameter that reflects skin water loss and barrier function. The lower the measured value, the better the skin barrier function. The detection method for trans-epidermal water loss analysis is as follows:

[0148] Subject screening criteria: healthy female subjects aged between 20 and 50 years old; and the subjects' skin tested positive after a 10% lactic acid sting test (determined to be sensitive skin); there was slight redness on the face; 90 subjects, the compositions obtained from Examples 1 to 8, Control Examples and Comparative Examples 1 to 9 were taken as samples for use evaluation, and 5 subjects were randomly selected for each sample.

[0149] Application area: face

[0150] Frequency of use: Use once in the morning and once in the evening for 28 consecutive days, 1.2g each time.

[0151] Test environment conditions: temperature 21±1℃, relative humidity 50±5%.

[0152] Experimental design: Single-blind, randomized, self-controlled before and after, face (the side opposite to the area where the lactic acid solution was applied was selected according to the random table).

[0153] Evaluation method details: The change rate of transepidermal water loss of facial skin was analyzed. The results are shown in Table 2 below.

[0154] Table 2. Statistical table of epidermal water loss test results for different cosmetic examples

[0155]

[0156] From the data in Table 2, within the range of Examples 1-8, as the mass percentage of glycerol glucoside, acetylhydroxyproline and panthenol in the formula gradually increases, the transepidermal water loss of facial skin shows an improving trend. Comparing Example 2 with Comparative Examples 1-9, it can be found that the effect of the combination of glycerol glucoside, acetylhydroxyproline and panthenol from desert algae is significantly better, which shows that the improvement effect of these ingredients alone or in simple combination on transepidermal water loss of facial skin is not as good as the combination of the three, and this combination achieves a positive effect on skin barrier repair. It can be seen from Comparative Example 8 that the barrier repair effect of glycerol glucoside prepared by other methods is obviously poor. The present invention can obtain high-purity glycerol glucoside by selecting desert algae as the extraction source, thereby significantly improving the barrier repair performance. It can be seen from Comparative Example 9 that the content of glycerol glucoside from other algae sources is low, much lower than that of desert algae. Due to the low concentration of the extracted glycerol glucoside, it is difficult to fully exert its barrier repair effect.

[0157] Efficacy test 2: Analysis of water content in the stratum corneum of the skin

[0158] The larger the value of the stratum corneum moisture content measured by Comneometer CM825, the higher the moisture content of the stratum corneum. By comparing the changes in stratum corneum moisture content in a specific area before and after use, the effect of the product on stratum corneum moisture can be reflected.

[0159] The more the moisture content at different revisit time points increases compared to the baseline value before use (D0), the better the improvement effect of the surface moisture content in the test area, indicating that the product is more effective in increasing the moisture content of the skin stratum corneum. Skin moisture content increase rate = (moisture content after using the product - moisture content before using the product) / moisture content before using the product × 100%

[0160] The screening criteria for subjects are the same as those for the analysis of transepidermal water loss

[0161] Evaluation method details: The change rate of transepidermal water loss of facial skin was analyzed. The results are shown in Table 3 below.

[0162] Table 3. Statistical table of skin stratum corneum moisture content test results for different cosmetic examples

[0163]

[0164]

[0165] It can be clearly seen from the data in Table 3 that, within the range of Examples 1-8, as the mass percentages of glyceryl glucoside, acetylhydroxyproline and panthenol in the formula continue to increase, the moisture content of the facial stratum corneum shows a good improvement trend.

[0166] Specifically, in the examples, the stratum corneum moisture content ratios showed positive growth at both 14 days and 28 days. For example, in Example 1, the stratum corneum moisture content ratio was 6.51% at 14 days and 8.52% at 28 days; in Example 8, the stratum corneum moisture content ratio was as high as 48.32% at 14 days and 58.80% at 28 days, which fully demonstrates that the stratum corneum moisture content is significantly increased with the increase in the mass proportion of the three components.

[0167] On the other hand, the change range of the stratum corneum moisture content ratio in the control group and comparative examples 1-9 is much smaller than that in the embodiment. For example, in the control group, the stratum corneum moisture content ratio is -1.5% at 14 days and -2.75% at 28 days; in comparative example 7, it is only 0.04% at 14 days and 1.05% at 28 days. These data comparisons further highlight the positive role of the combination of glycerol glucoside, acetylhydroxyproline and panthenol from desert algae in improving the moisture content of the facial stratum corneum, and provide a strong basis for the optimization of the moisturizing effect of skin care products. It can be seen from comparative example 8 that the moisturizing effect of glycerol glucoside prepared by other methods is obviously poor. The present invention can obtain high-purity glycerol glucoside by selecting desert algae as the extraction source, thereby significantly improving the moisturizing performance. It can be seen from comparative example 9 that the glycerol glucoside content of other algae sources is low, much lower than that of desert algae. Due to the low concentration of the extracted glycerol glucoside, it is difficult to fully exert its moisturizing effect.

[0168] Experiment 3: Analysis of the area of ​​red skin

[0169] The red area images taken by VISIA-CR are analyzed by the color spot analysis in the comprehensive skin analysis module of Image Pro Pus software to measure the changes in the skin red area ratio in the AOI selection area (radius 3.5cm) at different time points. By comparing the changes in the skin red area ratio before and after use, the soothing effect of the product can be reflected. The more the red area ratio decreases at different revisit time points compared with the baseline value before use (D0), the smaller the skin redness area, indicating that the product has a better effect in alleviating redness.

[0170] Formula: Red area percentage reduction rate = (red area percentage before using the product - red area percentage after using the product) / red area percentage before using the product x 100%

[0171] Subject screening criteria: healthy female subjects aged between 20 and 50 years old; and the subjects' skin tested positive after a 10% lactic acid sting test (determined to be sensitive skin); there was slight redness on the face; 90 subjects, the compositions obtained from Examples 1 to 8, Control Examples and Comparative Examples 1 to 9 were taken as samples for use evaluation, and 5 subjects were randomly selected for each sample.

[0172] Application area: face

[0173] Frequency of use: Use once in the morning and once in the evening for 28 consecutive days, 1.2g each time.

[0174] Test environment conditions: temperature 21±1℃, relative humidity 50±5%.

[0175] Experimental design: Single-blind, randomized, self-controlled before and after, face (the side opposite to the area where the lactic acid solution was applied was selected according to the random table).

[0176] Please refer to Table 4 for the test results:

[0177] Table 4. Statistical table of skin red area test results for different cosmetic examples

[0178]

[0179]

[0180] From the data in Table 4, it can be found that within the range of Examples 1-8, as the mass percentages of glyceryl glucoside, acetyl hydroxyproline and panthenol in the formula continue to increase, the facial red area shows a significant improvement trend.

[0181] Specifically, in Examples 1-8, the skin red area ratio decreased significantly from D0 to D28. For example, in Example 1, the skin red area ratio decreased from 75.85 to 69.46, a decrease of 8.42%; in Example 8, it decreased from 87.23 to 58.94, a decrease of up to 32.43%. This obvious downward trend fully demonstrates that the redness of the facial skin is effectively improved with the increase in the mass ratio of these three components.

[0182] Comparing the control example with comparative examples 1-9, the change in the percentage of the skin red area is far less than that of the embodiment. In the control example, the percentage of the skin red area increased from 48.15 to 49.42, an increase of 2.64%; in comparative example 7, it decreased from 69.34 to 68.90, only a decrease of 0.63%. These data further highlight the positive effect of the combination of glyceryl glucoside, acetylhydroxyproline and panthenol from desert algae in improving the condition of facial red areas, and provide a strong basis for the research and development and optimization of skin care products in soothing skin redness.

[0183] In summary, the strong barrier repair ingredient provided by the present invention provides efficient barrier repair from multiple aspects such as moisturizing, lipid repair, and anti-inflammatory relief through the synergistic effects of glycerol glucoside, acetylhydroxyproline, and panthenol derived from desert algae. The combination of these three ingredients complements each other in barrier repair, forming a comprehensive barrier repair solution that is suitable for skin care needs for damaged, sensitive, and dry barriers. The strong barrier repair ingredient of the present invention can not only meet the basic needs of barrier repair, but also provide multi-effect and long-lasting barrier protection. The strong barrier repair ingredient provided by the present invention can be applied to various cosmetics and skin care products.

[0184] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A strong screen repairing ingredient, characterized in that: The components thereof include, by weight, 0.001-20 parts of glycerol glucoside extracted from desert algae, 0.001-10 parts of acetylhydroxyproline and 0.001-20 parts of panthenol.

2. The screen repairing component according to claim 1, characterized in that: The components include, by mass percentage, 0.01-10 parts of glycerol glucoside extracted from desert algae, 0.01-8 parts of acetylhydroxyproline and 0.01-10 parts of panthenol; Preferably, the components include, by mass percentage, 0.1-5 parts of glycerol glucoside extracted from desert algae, 0.05-5 parts of acetylhydroxyproline and 0.1-5 parts of panthenol.

3. A method for preparing a strong screen repairing component, characterized in that: The method comprises uniformly mixing the components of the strong screen repairing ingredient according to any one of claims 1 to 2, wherein the preparation method of the glycerol glucoside extracted from desert algae comprises: The powder of desert algae is mixed with an ethanol solution, and ultrasonic extraction is performed at a temperature of 30° C. to 40° C. for 20 to 40 minutes, and the extraction is repeated 2 to 4 times, and the extracts are combined and centrifuged, and the supernatant is concentrated under reduced pressure to 15 to 25% of the original volume to obtain a concentrated solution, and the concentrated solution is separated by C18 reverse phase silica gel column chromatography, and gradient elution is performed using water and ethanol, and the eluate containing glycerol glucoside is collected; The eluate is placed in an ultrafiltration membrane system with a molecular weight cutoff value of 3000Da for filtration to obtain a filtrate, and the filtrate is cooled to 3-5°C and allowed to stand to induce crystallization of glycerol glucoside to obtain glycerol glucoside powder with a purity of ≥95%.

4. The method for preparing the strong screen repairing component according to claim 3, characterized in that: The desert algae include at least one of Chlorella vulgaris, Microcoleus scaly, Hasa algae and Cyanobacteria; Preferably, the mass ratio of the desert algae powder to the ethanol solution is 1:8-12, and the concentration of the ethanol solution is 70-80%; Preferably, the centrifugal stirring speed is 3000-10000 rpm, and the centrifugal time is 8-15 min.

5. The method for preparing the strong screen repairing component according to claim 3, characterized in that: The gradient elution comprises: (1) Loading: Slowly add the dissolved concentrate to a C18 reverse phase silica gel column, use ethanol as an eluent to press the sample onto the silica gel, and adjust the flow rate and mobile phase; (2) Elution: Use pure water, 30% ethanol, 50% ethanol, 70% ethanol and 100% ethanol in turn for elution in separate tubes or bottles; (3) Treatment of the eluate: Take a portion of the eluate from each tube or bottle and place it in a test tube. Place it in a centrifugal concentrator for concentration and then add 0.5 mL of ethanol to re-dissolve it. Then perform thin layer chromatography analysis. Combine the components with the same Rf value and consistent color development and collect the eluate containing glycerol glucoside.

6. Use of the strong screen repairing ingredient as described in any one of claims 1 to 2 or the strong screen repairing ingredient prepared by the preparation method of the strong screen repairing ingredient as described in any one of claims 3 to 5 in the preparation of cosmetics.

7. A strong screen repair cosmetic, characterized in that: It includes the strong screen repair component as described in any one of claims 1-2 or the strong screen repair component prepared by the preparation method of the strong screen repair component as described in any one of claims 3-5. Based on the total mass of the strong screen repair cosmetics as 100%, the content of the strong screen repair component is 0.003-50%.

8. The screen-repairing cosmetic according to claim 7, characterized in that: The strong screen repair cosmetics also include at least one of an emulsifier, an antioxidant, an emollient, a thickener, a moisturizer, a chelating agent, a fragrance, a preservative and a pH adjuster and the balance is water; Preferably, based on the total mass of the strong screen repair cosmetic as 100%, the content of the emulsifier is 0-20%, the content of the antioxidant is 0-10%, the content of the emollient is 0-50%, the content of the thickener is 0.01%-8%, the content of the moisturizer is 0.01%-40%, the content of the chelating agent is 0.01%-1%, the content of the flavoring agent is 0-1%, the content of the preservative is 0.01-3%, and the content of the pH adjuster is 0-3%; Preferably, the emulsifier is selected from one or more of PEG-100 stearate, polyglyceryl-3 methyl glucose distearate, potassium cetyl phosphate, cetearyl glucoside, cetearyl olivetate, sodium methyl stearoyl taurate, sodium methyl stearoyl taurate, hydrogenated lecithin, sorbitan olivetate and polyglyceryl-3 distearate; And / or, the antioxidant is selected from one or more of vitamin E and its derivatives, vitamin C and its derivatives, ubiquinone and fullerene; And / or, the emollient is selected from one or more of tri(ethylhexanoin)glyceryl, caprylic / capric triglyceride, pentaerythritol tetra(ethylhexanoin), hydrogenated polyisobutene, butyloctanol salicylate, diethoxyethyl succinate, isododecane, rice germ oil, hydrogenated palm kernel oil, cocoa seed butter cetyl ethylhexanoate, isopropyl myristate, isopropyl palmitate, diisopropyl sebacate, polydimethylsiloxane, cetearyl alcohol, dibutyl adipate, C12-15 alcohol benzoate, dicaprylyl carbonate, coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, caprylyl polymethylsiloxane, cyclopentasiloxane and phenyl trimethicone; And / or, the thickener is selected from one or more of xanthan gum, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, carbomer, ammonium acryloyldimethyltaurate / VP copolymer and sodium acrylate / sodium acryloyldimethyltaurate copolymer; And / or, the humectant is selected from one or more of glycerol, 1,3-propylene glycol, butylene glycol, dipropylene glycol, pentylene glycol, sorbitol, erythritol, 1,2-hexanediol, glyceryl polyether-26 and caprylyl glycol; And / or, the chelating agent is selected from one or a combination of two or more of disodium EDTA, tetrasodium EDTA and sodium phytate; And / or, the flavoring agent is selected from one or both of essence and essential oil; and / or, the preservative is selected from one or more of phenoxyethanol, p-hydroxyacetophenone, sodium benzoate, phenoxyethanol, methylparaben, caprylhydroxamic acid and chlorphenesin; And / or, the pH regulator is selected from one or more of arginine, sodium hydroxide, potassium hydroxide, triethanolamine and aminomethyl propanol.

9. The screen-repairing cosmetic according to claim 7, characterized in that: The strong screen repair cosmetics are selected from any one of skin care water, essence, lotion, face cream, face mask, sunscreen, BB cream and isolation cream.

10. A method for preparing the screen-repairing cosmetic according to any one of claims 7 to 9, characterized in that: The following steps are involved: S1: Stir and heat water, thickener, humectant, chelating agent, emollient and emulsifier to 80-90°C in sequence and mix well; S2: Add pH adjuster and mix well; S3: Cool down to 40-50℃, add preservatives and strong screen repair ingredients, mix evenly, and discharge.

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