Application of composition in preparation of preparation for improving sleep

Abstract

The invention discloses application of a composition in preparation of a preparation for improving sleep. The composition comprises tert-butylhydroquinone and linoleic acid. It is found that sleep cannot be improved when tert-butylhydroquinone is used alone or linoleic acid is used alone, but the composition of tert-butylhydroquinone and linoleic acid can play a role in improving sleep, increase the total sleep duration and the single sleep duration, and reduce the number of times of awakening at night and the number of times of autonomous activities at night. The composition disclosed by the invention is safe and easy to obtain, has a relatively good sleep improving effect and has a wide application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of improving sleep, and in particular to an application of a composition in preparing a preparation for improving sleep. Background Art

[0002] Sleep is a key physiological process regulated by the central nervous system and is essential for the body's rest and physical recovery. Sleep not only plays an important role in the maturation and development of the brain, but also has an irreplaceable position in maintaining physical and mental health and cognitive function. Insomnia is one of the most common sleep disorders, characterized by recurrent difficulty falling asleep, maintaining sleep, or both, and leading to dissatisfaction with sleep quality. Long-term insomnia has a significant impact on an individual's daily life and work, and increases the risk of various health problems. Severe lack of sleep can significantly reduce the patient's work efficiency and alertness, and may even lead to the occurrence of malignant accidents, bringing huge social and economic losses. At present, the drugs used clinically to treat insomnia mainly include benzodiazepine receptor agonists, melatonin receptor agonists, orexin receptor antagonists, and some antidepressants with hypnotic effects. Although prescription drugs have a good effect on improving sleep, the long-term effect is unclear, and there are obvious adverse reactions, including dependence, cognitive dysfunction, drug tolerance, and withdrawal reactions. Therefore, it is of great significance to provide a preparation that helps to improve sleep.

[0003] Linoleic acid is a polyunsaturated fatty acid of the ω-6 series. It is an essential nutrient for the human body but cannot be synthesized by itself. Linoleic acid has multiple biological functions in the body. As an important component of cell membrane phospholipids, linoleic acid plays an important role in maintaining the stability and function of cell structure. In addition, it is also involved in a variety of physiological processes in the body, including maintaining skin health, supporting immune function, and regulating blood pressure. However, studies have shown that in a multivariate adjustment model, for every standard deviation increase in linoleic acid, the odds of poor sleep quality, insufficient or excessive sleep, and a high risk of obstructive sleep apnea will increase (PMID: 37451068).

[0004] Tert-butylhydroquinone (TBHQ), also known as tert-butylhydroquinone, can enhance the stability of oil during its shelf life. TBHQ can be used as a food additive. It does not produce any odor when added to any oil or oil-containing food. Its safety is widely recognized and is listed as a safe A(1) product and is widely used in the food industry. Summary of the invention

[0005] In order to overcome the above defects and shortcomings in the prior art, the present invention provides an application of a composition in the preparation of a preparation for improving sleep.

[0006] The first object of the present invention is to provide a composition for use in preparing a preparation for improving sleep.

[0007] A second object of the present invention is to provide a preparation for improving sleep.

[0008] Therefore, the present invention claims the following:

[0009] The invention discloses an application of a composition in preparing a preparation for improving sleep, wherein the composition comprises tert-butylhydroquinone and linoleic acid.

[0010] Preferably, the mass ratio of tert-butylhydroquinone to linoleic acid is 1 g:(5-25) g.

[0011] More preferably, the mass ratio of tert-butylhydroquinone to linoleic acid is 1g:25g.

[0012] Preferably, the improving sleep is to increase the total sleep duration.

[0013] Preferably, the improving sleep is to increase the duration of a single sleep.

[0014] Preferably, the improvement of sleep is to reduce the number of awakenings at night.

[0015] Preferably, the improvement of sleep is to reduce the number of autonomous activities at night.

[0016] As an practicable manner, the preparation is in the form of solid, semi-solid or liquid.

[0017] As an practicable manner, the dosage form of the preparation includes but is not limited to tablets, capsules, granules, powders and pills.

[0018] As an practicable manner, the preparation includes but is not limited to medicines and health foods, the medicines also include pharmaceutically acceptable excipients, and the health foods also include food-acceptable additives.

[0019] A preparation for improving sleep, comprising tert-butylhydroquinone and linoleic acid.

[0020] Preferably, the mass ratio of tert-butylhydroquinone to linoleic acid is 1 g:(5-25) g.

[0021] More preferably, the mass ratio of tert-butylhydroquinone to linoleic acid is 1g:25g.

[0022] Preferably, the improving sleep is to increase the total sleep duration.

[0023] Preferably, the improving sleep is to increase the duration of a single sleep.

[0024] Preferably, the improvement of sleep is to reduce the number of awakenings at night.

[0025] Preferably, the improvement of sleep is to reduce the number of autonomous activities at night.

[0026] As an practicable manner, the preparation is in the form of solid, semi-solid or liquid.

[0027] As an practicable manner, the dosage form of the preparation includes but is not limited to tablets, capsules, granules, powders and pills.

[0028] As an practicable manner, the preparation includes but is not limited to medicines and health foods, the medicines also include pharmaceutically acceptable excipients, and the health foods also include food-acceptable additives.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The present invention discloses an application of a composition in preparing a preparation for improving sleep, wherein the composition comprises tert-butylhydroquinone and linoleic acid. The present invention finds that the use of tert-butylhydroquinone alone and linoleic acid alone cannot improve sleep, while the combination of tert-butylhydroquinone and linoleic acid can improve sleep, increase the total sleep time and the single sleep time, and reduce the number of awakenings at night and the number of autonomous activities at night. The composition of the present invention is safe and easy to obtain, has a good effect of improving sleep, and has broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is the effect of linoleic acid on the total sleep duration of fruit flies.

[0032] Figure 2 This is the effect of linoleic acid on the sleep frequency of fruit flies.

[0033] Figure 3 This is the effect of linoleic acid on the duration of a single sleep session in fruit flies.

[0034] Figure 4 The effect of linoleic acid on the number of nocturnal activities of fruit flies.

[0035] Figure 5 The effect of the combination of TBHQ and linoleic acid on the total sleep duration of Drosophila.

[0036] Figure 6 The effect of the combination of TBHQ and linoleic acid on the sleep frequency of Drosophila.

[0037] Figure 7 The effect of the combination of TBHQ and linoleic acid on the duration of single sleep in fruit flies.

[0038] Figure 8The effect of the combination of TBHQ and linoleic acid on the frequency of nocturnal activity in Drosophila.

[0039] Fig. 9 This is a comparison of the effects of linoleic acid alone, TBHQ alone, and a combination of linoleic acid and TBHQ on improving sleep in fruit flies. DETAILED DESCRIPTION

[0040] The present invention is further described below with reference to specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0041] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0042] Example 1 Effect of linoleic acid on Drosophila sleep

[0043] 1. Experimental Methods

[0044] 1. Experimental animals and feeding

[0045] Wild-type Drosophila melanogaster (Canton S) was obtained from the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. The fruit flies were raised in an artificial climate chamber (temperature set at 25°C, relative humidity set at 60%), with 12 hours of day and 12 hours of night, with a day-night reciprocating cycle.

[0046] 2. Preparation of Drosophila Experimental Medium

[0047] Basic culture medium: 2.5 mL of propionic acid, 5 g of agar, 25 g of sucrose, and 500 mL of water. Accurately weigh sucrose and agar in a beaker, transfer them into an iron pot, add water, heat to boiling, cool after cooking, add propionic acid, and stir evenly.

[0048] Caffeine medium: On the basis of the basic medium, caffeine is added according to the mass ratio to prepare a medium containing 0.05% caffeine (w / w).

[0049] Caffeine medium containing 0.05% linoleic acid (w / w): On the basis of caffeine medium, linoleic acid (brand: McLean, item number: L812256) was added according to the mass ratio to prepare a caffeine medium containing 0.05% linoleic acid (w / w).

[0050] Caffeine medium containing 0.25% linoleic acid (w / w): On the basis of caffeine medium, linoleic acid is added according to the mass ratio to prepare a caffeine medium containing 0.25% linoleic acid (w / w).

[0051] 3. Drosophila sleep monitoring

[0052] Male fruit flies born within 8 hours were selected and placed in basal culture medium for normal culture for 3 days. After 3 days, they were placed in fruit fly activity monitoring tubes containing different intervention culture mediums, and then the fruit fly activity monitoring tubes were placed in a fruit fly activity monitor (Trikinetics, USA, model DAM5). The specific experimental groups are shown in Table 1.

[0053] Table 1 Test groups

[0054] Group Number of fruit flies (only) Culture medium Normal group ≥16 Basic culture medium Caffeine group ≥16 Caffeine medium Low-dose linoleic acid group ≥16 Caffeine medium containing 0.05% linoleic acid (w / w) High-dose linoleic acid group ≥16 Caffeine medium containing 0.25% linoleic acid (w / w)

[0055] Each group was equipped with a fruit fly activity monitor, and each fruit fly activity monitor corresponded to 32 separate fruit fly activity monitoring tubes. A fruit fly was placed in each tube, with culture medium placed at one end and a breathable plug at the other end. The tube was placed in a constant temperature and humidity incubator and connected to the fruit fly activity monitor to continuously monitor the activity behavior and sleep state of the fruit flies for 3 days. The sleep state of fruit flies can be defined by their activity behavior, that is, any period of inactivity lasting 5 minutes or more is considered to be a sleep state for the fruit fly.

[0056] Each fruit fly activity monitor can monitor the activities of 32 fruit flies at the same time. The fruit fly activity monitor has a total of 4 infrared beams to monitor the activities of fruit flies. The fruit fly activity monitor emits infrared beams from one side and receives infrared beams from the other side. When the fruit fly is active in the monitoring tube, it will block the reception of the infrared beam. Whether the infrared beam is blocked will be transmitted back to the computer at a frequency of 1 time / min to monitor the activity status of the fruit fly. The monitoring data is transmitted to the corresponding computer in real time. The sleep state of the fruit fly is determined by its behavior, that is, any period of inactivity lasting 5 minutes or more. The number of fruit flies' nighttime activities, total sleep duration, number of sleeps and single sleep duration are used as evaluation indicators for continuous monitoring for 3 days, and all monitoring is carried out in a dark environment.

[0057] 4. Data Processing and Analysis

[0058] For the Drosophila sleep research experiment, GraphPad Prism 9.0 software was used to draw pictures, and SPSS 20.0 software was used for statistical analysis of the data. Depending on whether the variance was homogeneous, the LeastSignificant Difference test or Tamhane's T2(M) in the one-way analysis of variance and the non-parametric test Kruskal-Wallis were selected. Data are expressed as Mean±SEM, * indicates p<0.05 compared with the normal group, ** indicates p<0.01 compared with the normal group; # indicates p<0.05 compared with the caffeine group, and ## indicates p<0.01 compared with the caffeine group.

[0059] 2. Experimental Results

[0060] 1. Effect of linoleic acid on the total sleep duration of fruit flies

[0061] The results are as follows Figure 1 On the first day, there was no significant change in the caffeine group compared with the normal group of fruit flies; compared with the caffeine group, there was no significant change trend in the total sleep duration of fruit flies in the low-dose linoleic acid group and the high-dose linoleic acid group.

[0062] On the second day, the total sleep duration of the fruit flies in the caffeine group was significantly reduced compared with the fruit flies in the normal group (p < 0.05). Compared with the caffeine group, the total sleep duration of the fruit flies in the low-dose linoleic acid group and the high-dose linoleic acid group had no significant change trend.

[0063] On the third day, the total sleep duration of the caffeine group was significantly reduced compared with the normal group (p < 0.01). Compared with the caffeine group, the total sleep duration of the low-dose linoleic acid group and the high-dose linoleic acid group had no significant change trend.

[0064] The above results indicate that linoleic acid at a specific dose cannot reverse the caffeine-induced shortening of total sleep duration in fruit flies, and fails to prolong the total sleep duration of insomniac fruit flies.

[0065] 2. Effect of linoleic acid on the sleep frequency of fruit flies

[0066] The results are as follows Figure 2 On the first day, compared with the normal group of fruit flies, the sleep frequency of fruit flies in the caffeine group increased significantly (p < 0.01), indicating that caffeine intervention began to induce fragmented sleep in fruit flies, resulting in a decrease in their sleep quality; compared with the caffeine group, the sleep frequency of fruit flies in the low-dose linoleic acid group and the high-dose linoleic acid group did not change significantly.

[0067] On the second day, compared with the normal group of fruit flies, the sleep frequency of fruit flies in the caffeine group increased significantly (p < 0.01), and compared with the caffeine group, the sleep frequency of fruit flies in the low-dose linoleic acid group and the high-dose linoleic acid group did not change significantly.

[0068] On the third day, compared with the normal group of fruit flies, the sleep frequency of fruit flies in the caffeine group increased significantly (p < 0.01), and compared with the caffeine group, the sleep frequency of fruit flies in the low-dose linoleic acid group and the high-dose linoleic acid group did not change significantly.

[0069] The above results indicate that linoleic acid at a specific dose failed to reverse caffeine-induced sleep fragmentation in fruit flies and failed to reduce the number of night sleeps in insomniac fruit flies.

[0070] 3. Effect of linoleic acid on the duration of single sleep in fruit flies

[0071] The results are as follows Figure 3As shown. On the first day, compared with the normal group of fruit flies, the single sleep duration of the caffeine group fruit flies was significantly reduced (p < 0.01). Compared with the caffeine group, the single sleep duration of fruit flies in the low-dose linoleic acid group and the high-dose linoleic acid group did not change significantly.

[0072] On the second day, compared with the normal group, the single sleep duration of the caffeine group was significantly reduced (p < 0.01). Compared with the caffeine group, the single sleep duration of the low-dose linoleic acid group and the high-dose linoleic acid group did not change significantly.

[0073] On the third day, compared with the normal group, the single sleep duration of the caffeine group was significantly reduced (p < 0.01). Compared with the caffeine group, the single sleep duration of the low-dose linoleic acid group and the high-dose linoleic acid group did not change significantly.

[0074] The above results indicate that linoleic acid at a specific dose fails to increase the single sleep duration of insomniac fruit flies, thereby improving sleep quality.

[0075] 4. Effect of linoleic acid on the frequency of nocturnal activity of fruit flies

[0076] The results are as follows Figure 4 As shown. On the first day, the number of nocturnal activities of the fruit flies in the caffeine group increased compared with the fruit flies in the normal group. Compared with the caffeine group, the number of nocturnal activities of the fruit flies in the low-dose linoleic acid group and the high-dose linoleic acid group decreased.

[0077] On the second day, the number of nocturnal activities of the caffeine group fruit flies increased significantly compared with the normal group fruit flies (p < 0.05). Compared with the caffeine group, the number of nocturnal activities of the low-dose linoleic acid group and the high-dose linoleic acid group fruit flies showed a decreasing trend, but there was no significant change.

[0078] On the third day, the number of nocturnal activities of the fruit flies in the caffeine group increased significantly compared with the normal group (p < 0.05). Compared with the caffeine group, the number of nocturnal activities of the fruit flies in the low-dose linoleic acid group and the high-dose linoleic acid group did not change significantly.

[0079] The above results indicate that linoleic acid at a specific dose fails to reduce the number of nocturnal activities of insomniac fruit flies.

[0080] Example 2 Effect of the Combination of TBHQ and Linoleic Acid on Drosophila Sleep

[0081] 1. Experimental Methods

[0082] 1. Experimental animals and feeding

[0083] Proceed according to Example 1.

[0084] 2. Preparation of Drosophila Experimental Medium

[0085] The preparation methods of the basal culture medium and the caffeine culture medium are the same as those in Example 1.

[0086] Caffeine medium containing 0.01% TBHQ (w / w) and 0.05% linoleic acid (w / w): On the basis of caffeine medium, linoleic acid (brand: McLean, product number: L812256) and TBHQ were added according to the mass ratio to prepare a caffeine medium containing 0.01% TBHQ (w / w) and 0.05% linoleic acid (w / w).

[0087] Caffeine medium containing 0.01% TBHQ (w / w) and 0.25% linoleic acid (w / w): On the basis of caffeine medium, linoleic acid (brand: McLean, product number: L812256) and TBHQ were added according to the mass ratio to prepare a caffeine medium containing 0.01% TBHQ (w / w) and 0.25% linoleic acid (w / w).

[0088] 3. Drosophila sleep monitoring

[0089] The experiment was carried out according to Example 1, and the specific test groups are shown in Table 2.

[0090] Table 2 Experimental groups

[0091]

[0092] 4. Data Processing and Analysis

[0093] Proceed according to Example 1.

[0094] 2. Experimental Results

[0095] 1. Effect of the combination of TBHQ and linoleic acid on the total sleep duration of Drosophila

[0096] The results are as follows Figure 5 On the first day, compared with the normal group of fruit flies, the total sleep time of the fruit flies in the caffeine group was significantly reduced (p < 0.01); compared with the caffeine group, the total sleep time of the fruit flies in the low-dose linoleic acid group supplemented with TBHQ and the high-dose linoleic acid group supplemented with TBHQ showed an increasing trend, among which the high-dose linoleic acid group supplemented with TBHQ significantly increased the total sleep time of the fruit flies (p < 0.01).

[0097] On the second day, the total sleep duration of the caffeine group fruit flies was significantly reduced compared with the normal group fruit flies (p < 0.01). Compared with the caffeine group, the total sleep duration of the fruit flies in the low-dose linoleic acid group supplemented with TBHQ and the high-dose linoleic acid group supplemented with TBHQ was significantly increased (p < 0.05).

[0098] On the third day, the total sleep time of the fruit flies in the caffeine group was significantly reduced compared with the normal group (p < 0.01). Compared with the caffeine group, the total sleep time of the fruit flies in the low-dose linoleic acid group supplemented with TBHQ and the high-dose linoleic acid group supplemented with TBHQ showed an increasing trend, among which the high-dose linoleic acid group supplemented with TBHQ significantly increased the total sleep time of the fruit flies (p < 0.01).

[0099] The above results indicate that the combination of TBHQ and linoleic acid at a specific dose can reverse the caffeine-induced shortening of the total sleep duration of fruit flies, and can significantly prolong the total sleep duration of insomniac fruit flies, thereby improving sleep.

[0100] 2. Effect of the combination of TBHQ and linoleic acid on the sleep frequency of Drosophila

[0101] The results are as follows Figure 6 On the first day, compared with the normal group, the sleep frequency of the fruit flies in the caffeine group increased, indicating that caffeine intervention began to induce fragmented sleep in fruit flies, resulting in a decrease in sleep quality; compared with the caffeine group, the sleep frequency of the fruit flies in the low-dose linoleic acid group supplemented with TBHQ and the high-dose linoleic acid group supplemented with TBHQ decreased, and the sleep frequency of the fruit flies in the high-dose linoleic acid group supplemented with TBHQ was significantly reduced.

[0102] On the second day, compared with the normal group of fruit flies, the sleep times of fruit flies in the caffeine group increased significantly (p < 0.01). Compared with the caffeine group, the sleep times of fruit flies in the low-dose linoleic acid group with TBHQ and the high-dose linoleic acid group with TBHQ showed a decreasing trend, among which the high-dose linoleic acid group with TBHQ significantly reduced the sleep times of fruit flies (p < 0.05).

[0103] On the third day, compared with the normal group of fruit flies, the sleep times of fruit flies in the caffeine group increased significantly (p < 0.01). Compared with the caffeine group, the sleep times of fruit flies in the low-dose linoleic acid group with TBHQ and the high-dose linoleic acid group with TBHQ showed a decreasing trend, among which the high-dose linoleic acid group with TBHQ significantly reduced the sleep times of fruit flies (p < 0.05).

[0104] The above results indicate that the combination of TBHQ and linoleic acid at a specific dose can reverse caffeine-induced sleep fragmentation in fruit flies, and has the effect of significantly reducing the number of sleep times of insomniac fruit flies, thereby improving sleep quality.

[0105] 3. Effect of the combination of TBHQ and linoleic acid on the duration of single sleep in Drosophila

[0106] The results are as follows Figure 7As shown. On the first day, compared with the normal group of fruit flies, the single sleep duration of the fruit flies in the caffeine group showed a decreasing trend. Compared with the caffeine group, the sleep times of the fruit flies in the low-dose linoleic acid group supplemented with TBHQ and the high-dose linoleic acid group supplemented with TBHQ showed an increasing trend, among which the high-dose linoleic acid group supplemented with TBHQ significantly increased the single sleep duration of the fruit flies (p < 0.05).

[0107] On the second day, compared with the normal group, the single sleep duration of the caffeine group was significantly reduced (p < 0.01). Compared with the caffeine group, the sleep times of the low-dose linoleic acid group and the high-dose linoleic acid group with TBHQ showed an increasing trend, and the single sleep duration of the high-dose linoleic acid group with TBHQ was significantly increased (p < 0.05).

[0108] On the third day, compared with the normal group, the single sleep duration of the caffeine group was significantly reduced (p < 0.01). Compared with the caffeine group, the sleep times of the low-dose linoleic acid group and the high-dose linoleic acid group with TBHQ added showed an increasing trend, and the single sleep duration of the high-dose linoleic acid group with TBHQ added was significantly increased (p < 0.01).

[0109] The above results indicate that the combination of TBHQ and linoleic acid at a specific dose can increase the single sleep duration of insomniac fruit flies, thereby improving sleep quality.

[0110] 4. Effect of the combination of TBHQ and linoleic acid on the frequency of nocturnal activity of Drosophila

[0111] The results are as follows Figure 8 As shown. On the first day, compared with the normal group of fruit flies, the number of nocturnal activities of fruit flies in the caffeine group increased significantly (p < 0.01). Compared with the caffeine group, the number of nocturnal activities of fruit flies in the low-dose linoleic acid group supplemented with TBHQ and the high-dose linoleic acid group supplemented with TBHQ showed a decreasing trend, among which the number of nocturnal activities of fruit flies in the high-dose linoleic acid group supplemented with TBHQ decreased significantly (p < 0.01).

[0112] On the second day, compared with the normal group, the nighttime activity of the caffeine group increased. Compared with the caffeine group, the nighttime activity of the low-dose linoleic acid group supplemented with TBHQ and the high-dose linoleic acid group supplemented with TBHQ decreased, and the nighttime activity of the high-dose linoleic acid group supplemented with TBHQ decreased significantly (p < 0.01).

[0113] On the third day, compared with the normal group, the nighttime activity of the caffeine group was significantly increased (p < 0.05). Compared with the caffeine group, the nighttime activity of the low-dose linoleic acid group supplemented with TBHQ and the high-dose linoleic acid group supplemented with TBHQ showed a decreasing trend, and the nighttime activity of the high-dose linoleic acid group supplemented with TBHQ was significantly reduced (p < 0.01).

[0114] The above results indicate that the combination of TBHQ and linoleic acid at a specific dose can reduce the number of nocturnal activities of insomniac fruit flies to a certain extent, thereby inducing the occurrence of sleep.

[0115] Example 3 Comparison of the effects of using linoleic acid alone, TBHQ alone, and a combination of linoleic acid and TBHQ on improving sleep in Drosophila

[0116] 1. Experimental Methods

[0117] 1. Experimental animals and feeding

[0118] Proceed according to Example 1.

[0119] 2. Preparation of Drosophila Experimental Medium

[0120] The preparation methods of the basal medium, caffeine medium, caffeine medium containing 0.05% linoleic acid (w / w), and caffeine medium containing 0.25% linoleic acid (w / w) were the same as those in Example 1. The preparation methods of the caffeine medium containing 0.01% TBHQ (w / w) and 0.05% linoleic acid (w / w) and the caffeine medium containing 0.01% TBHQ (w / w) and 0.25% linoleic acid (w / w) were the same as those in Example 2.

[0121] Basic culture medium containing 0.01% TBHQ (w / w): TBHQ was added to the basic culture medium according to the mass ratio to prepare a basic culture medium containing 0.01% TBHQ (w / w).

[0122] Caffeine medium containing 0.01% TBHQ (w / w): On the basis of caffeine medium, TBHQ was added according to the mass ratio to prepare a caffeine medium containing 0.01% TBHQ (w / w).

[0123] 3. Drosophila sleep monitoring

[0124] The experiment was carried out according to Example 1, and the specific test groups are shown in Table 3.

[0125] Table 3 Experimental groups

[0126]

[0127] 4. Data Processing and Analysis

[0128] For the Drosophila sleep research experiment, GraphPad Prism 9.0 software was used to draw pictures, and SPSS 20.0 software was used for statistical analysis of the data. Depending on whether the variance was homogeneous, the LeastSignificant Difference test or Tamhane's T2(M) in the one-way analysis of variance and the non-parametric test Kruskal-Wallis were selected. The data are expressed as Mean±SEM, * indicates p<0.05 between groups, and ** indicates p<0.01 between groups.

[0129] 2. Experimental Results

[0130] The results are as follows Fig. 9 For the normal group, the addition of TBHQ did not significantly affect the average total sleep duration of fruit flies.

[0131] For the caffeine group, the addition of TBHQ did not significantly affect the average total sleep time of the fruit flies.

[0132] For the low-dose linoleic acid group, the average total sleep duration of fruit flies in the low-dose linoleic acid group supplemented with TBHQ was significantly higher than that in the low-dose linoleic acid group without TBHQ supplementation (p<0.05).

[0133] For the high-dose linoleic acid group, the average total sleep duration of fruit flies in the high-dose linoleic acid group supplemented with TBHQ was significantly higher than that in the high-dose linoleic acid group without TBHQ supplementation (p<0.01).

[0134] In summary, in the caffeine group and the normal group, the addition of TBHQ and the absence of TBHQ did not significantly affect the average total sleep duration of fruit flies. In the low-dose and high-dose linoleic acid groups, the addition of TBHQ significantly affected the average total sleep duration of fruit flies (p < 0.05). These results indicate that the combination of TBHQ and linoleic acid can improve sleep.

[0135] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. Use of a composition in preparing a preparation for improving sleep, characterized in that: The composition comprises tert-butylhydroquinone and linoleic acid.

2. The use according to claim 1, characterized in that: The mass ratio of the tert-butylhydroquinone to linoleic acid is 1 g: (5-25) g.

3. The use according to claim 1, characterized in that: The improving sleep is to increase the total sleep duration.

4. The use according to claim 1, characterized in that: The improving sleep is to increase the duration of a single sleep.

5. The use according to claim 1, characterized in that: The improved sleep refers to reducing the number of awakenings at night.

6. The use according to claim 1, characterized in that: The described improvement of sleep is to reduce the number of autonomous activities at night.

7. The use according to claim 1, characterized in that: The preparation is in the form of solid, semisolid or liquid.

8. The use according to claim 1, characterized in that: The dosage form of the preparation includes, but is not limited to, tablets, capsules, granules, powders and pills.

9. The use according to claim 1, characterized in that: The preparation includes but is not limited to medicines and health foods. The medicines also include pharmaceutically acceptable excipients, and the health foods also include food-acceptable additives.

10. A preparation for improving sleep, characterized in that: The formulation contains tert-butylhydroquinone and linoleic acid.

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