Traditional Chinese medicine mixed extract for regulating and tonifying qi and blood as well as preparation method and application of traditional Chinese medicine mixed extract

Abstract

The invention discloses a traditional Chinese medicine mixed extract for regulating and tonifying qi and blood as well as a preparation method and application thereof. The composition comprises water, an alcohol solvent and a traditional Chinese medicine mixed component, and the traditional Chinese medicine mixed component mainly comprises 5-15 parts of millettia speciosa, 1-10 parts of abelmoschus manihot and 5-15 parts of dendrobium nobile. The millettia specisoa, the abelmoschus manihot, the dendrobium nobile and the like are compounded together, the composition is prepared through a modern extraction process, and the composition has a synergistic effect, has the effects of promoting the secretion of saliva or body fluid, moistening dryness, replenishing qi, enriching blood, nourishing the lung, promoting blood circulation, promoting metabolism, scavenging free radicals, promoting collagen regeneration and improving skin vitality. When the composition is applied to a cosmetic formula matrix, the composition can complement qi and blood for middle-aged and elderly women, the qi and blood are full, the skin is ruddy, the face is glossy, the body health is ensured, and the appearance is beautiful.

Description

Technical Field

[0001] The invention relates to the field of extraction of Chinese medicinal ingredients, and in particular to a mixed Chinese medicinal extract for regulating qi and blood, and a preparation method and application thereof. Background Art

[0002] The Yellow Emperor's Classic of Internal Medicine states: "When a woman is 57 years old, her Yangming pulse weakens, her face starts to become sallow, and her hair starts to fall out." This means that from the age of 57 (that is, 35 years old), a woman's body will begin to go downhill, with problems such as facial decay, hair loss, and graying.

[0003] According to TCM theory, women are based on qi and blood, and their appearance also depends on the nourishment of qi and blood. "The heart controls blood, and its beauty is in the face. If qi and blood are sufficient, the complexion will be rosy. The lungs control qi, and its beauty is in the skin. If qi and blood are sufficient, the skin will be moisturized. The kidneys store essence, and its beauty is in the hair. Essence and blood have the same origin. If the essence and blood are sufficient, the hair will be black and shiny." "A woman's yangming pulse declines at the age of 57," which means that the body cannot get enough qi and blood, and the face and hair cannot get enough qi and blood to nourish them. Naturally, the face will start to become burnt and the hair will start to fall. If they are properly maintained and have sufficient qi and blood, they will age more slowly. Therefore, women must pay attention to replenishing qi and blood.

[0004] In today's society, people are under great pressure in work and life, and fatigue and exhaustion further increase the burden on the body. Therefore, the concept of TCM health care, which is to relax the muscles and tendons, promote blood circulation and remove blood stasis, warm and nourish the qi and blood, and regulate the discomfort of the whole body, has become a healthy choice for many people. Among them, the external use of Chinese herbal essential oils for warming and nourishing qi and blood is very popular because it does not require oral administration, is combined with massage, and nourishes qi and blood. The Chinese herbal essential oils for warming and nourishing qi and blood are guided by the theory of traditional Chinese medicine, with warming and nourishing qi and blood as the core, and with plants as the original ingredients, combined with modern technical means. The external use of Chinese herbal extracts for warming and nourishing qi and blood has its requirements, that is, it must be able to effectively play the role of warming and nourishing qi and blood, and it must not irritate the human skin.

[0005] The common essential oils for warming and nourishing qi and blood on the market are often single Chinese herbal extracts, with single warming and nourishing herbs and mediocre efficacy; or they are mixed with several Chinese herbal extracts, without sufficient contact between the drugs, and the synergistic warming and nourishing effects of multiple drugs cannot be fully exerted.

[0006] Patent CN108815373A discloses a Chinese herbal medicine for relaxing tendons and activating collaterals essential oil and its preparation method, the components include: papaya oil, parasitic oil, Polygonatum odoratum oil, Dipsacus asper oil, Rhizoma Cynoglossi oil, Angelica sinensis oil, Rhizoma Chuanxiong oil, Safflower oil, Angelica dahurica oil, Notopterygium wilfordii oil, Saposhnikovia divaricata oil, Atractylodes macrocephala oil, Cinnamomum cassia oil, Clematidis oil, Licorice oil, Atractylodes lancea oil, Poria oil, Gentiana macrophylla oil, Drynaria fortunei oil, Caulis Piperis oil, Caulis Sinensis oil, Rhizoma Dioscoreae oil, Geranium oil, Solanum root oil, the balance is base oil. The preparation method of the Chinese herbal medicine for relaxing tendons and activating collaterals essential oil is to slowly stir at a stirring speed of 300-350r / min for 45-60min at 23-25°C. Most of these more than 20 kinds of essential oils do have the effects of dispelling wind and dampness, relaxing tendons and activating collaterals, but simple physical mixing at room temperature cannot ensure the reasonable compatibility of the active ingredients of each essential oil.

[0007] In addition, most of the common Chinese herbal essential oils on the market are extracted with ethanol, which may have ethanol residues and other harmful components. When Chinese herbal essential oils containing ethanol or harmful components are applied to the human epidermis, they may irritate the skin. Patent CN108165372A discloses a method for extracting ginseng essential oil, which first extracts ginseng active ingredients with 10% ethanol, then adds NaOH to adjust the pH to 7, and then enzymolyzes for 3-4 hours, and then removes the filtrate after filtration. The filtrate is dealcoholized, concentrated, and dried to obtain ginseng essential oil. This invention solves the problems of low yield and high impurities of traditional ginseng essential oil, but the final product may have residual NaOH and other potential irritants to the skin. Summary of the invention

[0008] To solve the problems existing in the above-mentioned background technology, the present invention provides a mixed Chinese medicine extract for regulating qi and blood, and its ingredients include mixed Chinese medicine components.

[0009] The traditional Chinese medicine mixed components mainly include: 5-15 parts of Millettia chinensis, 1-10 parts of Helichrysum chrysantha, and 5-15 parts of Dendrobium nobile.

[0010] Furthermore, the Chinese medicine mixed components can also include: 8-12 parts of ginseng, 5-8 parts of Poria cocos, 5-8 parts of Atractylodes macrocephala, 7-10 parts of licorice, 7-10 parts of Chuanxiong, 7-10 parts of Rehmannia glutinosa, 7-10 parts of Angelica sinensis, 3-5 parts of white peony root, 5-8 parts of Codonopsis pilosula, 5-8 parts of cinnamon, 7-10 parts of dried ginger, 8-12 parts of Astragalus and 8-12 parts of Dendrobium officinale.

[0011] The present invention also provides a method for preparing any of the above-mentioned mixed extracts of traditional Chinese medicines for regulating qi and blood, comprising the following preparation steps: Step (1): weighing the Chinese medicine components according to the number, mixing, and crushing into medicine powder; mixing the medicine powder, water, and cellulase in a weight ratio of 100:600:3, hydrolyzing at 50-55° C. for 3-5 hours, and inactivating the enzyme in a boiling water bath for 10 minutes to obtain a hydrolyzate; Step (2): mixing the hydrolyzate and Lactobacillus casei, wherein the amount of Lactobacillus casei added is 1% of the weight of the hydrolyzate; and fermenting at room temperature for 2-4 days to obtain a fermented product; Step (3): adding ethanol to the fermented product until the ethanol volume concentration reaches 75%, soaking for more than 0.5 hours to obtain a Chinese medicine soaking liquid; Step (4): using a condensation reflux device to condense and reflux the Chinese medicine soaking liquid for 2-3 hours, filtering the condensed refluxed object to remove the drug residue, and obtaining the Chinese medicine ethanol extract; Step (5): uniformly mixing the Chinese medicine ethanol extract and the alcohol solvent in a mass ratio of 1:1 to obtain a mixed solution; Step (6): The mixed liquid is subjected to rotary evaporation using a rotary evaporator until no more liquid in the condenser flows into the receiving bottle, and the liquid remaining in the evaporation bottle is the mixed extract of Chinese medicine for regulating qi and blood, which contains water, alcohol solvent and mixed components of Chinese medicine.

[0012] Furthermore, the condensation reflux process in step (4) is to extract the active ingredients of various drugs with ethanol; further, the various active ingredients may undergo chemical reactions such as complexation and synthesis with each other, and are in close contact and mutual penetration with each other; further, the heating temperature used in the condensation reflux process is 70-90°C.

[0013] Furthermore, the pore size of the filter membrane used in the filtration process in step (4) is 200-600 mesh, preferably 400-600 mesh.

[0014] Furthermore, the purpose of the rotary evaporation process in step (6) is to volatilize and remove ethanol; further, the alcohol solvent can replace ethanol as the solvent of the mixed extract of traditional Chinese medicine, so that ethanol can be volatilized and removed more easily and thoroughly; further, the heating temperature used in the rotary evaporation process is 50-80°C, and the vacuum degree is 0.05-0.1MPa.

[0015] Furthermore, the alcohol solvent is isoprene glycol.

[0016] The isoprene glycol described in the present invention is composed of isoprene and two water molecules, has a primary alcohol and a tertiary alcohol in the molecular structure, has no irritation to eyes and skin, has good compatibility with various raw materials, and compared with other polyols, is not only easily soluble in water, but also has excellent oil compatibility and is compatible with non-polar solvents such as benzene, and has a refreshing and non-greasy moisturizing feeling.

[0017] The mixed Chinese herbal extract for regulating qi and blood of the present invention can be added to a cosmetic matrix as an active ingredient, and the added amount can be 1-10%. The cosmetic matrix can be a conventional skin care or hair care product, such as cream, lotion, water solution, essence, facial mask, shampoo, conditioner, etc.

[0018] The beneficial effects of the present invention are remarkable. Firstly, the Chinese medicinal ingredients used have the following effects: Beautiful Millettia vine: replenishes the lungs, nourishes the kidneys and strengthens yang, relaxes muscles and relieves pain, moistens the intestines and promotes bowel movements, soothes the liver and replenishes deficiency.

[0019] Golden sunflower: clears dampness and heat, reduces inflammation and relieves pain.

[0020] Dendrobium candidum: nourishes yin, clears heat, promotes fluid production and quenches thirst.

[0021] Cinnamon: replenishes fire and strengthens yang, guides fire back to its origin, dispels cold and relieves pain, warms and unclogs the meridians.

[0022] Ginseng: Greatly replenishes vital energy, nourishes the spleen and lungs, promotes fluid production and quenches thirst, calms the mind and improves intelligence.

[0023] Angelica: replenishes blood and promotes blood circulation, regulates menstruation and relieves pain, moistens the intestines and promotes bowel movements.

[0024] Chuanxiong: promotes blood circulation and qi movement, dispels wind and relieves pain.

[0025] Atractylodes: tonifies Qi and invigorates dampness, dries dampness and promotes diuresis, stops sweating, and stabilizes the fetus.

[0026] Codonopsis: tonifies the middle and replenishes Qi, promotes the production of body fluids and nourishes blood.

[0027] Poria: promotes diuresis and eliminates dampness, strengthens the spleen, and calms the mind.

[0028] Licorice: replenishes Qi and strengthens the middle, eliminates phlegm and quenches thirst, detoxifies, relieves pain, and moderates the properties of drugs.

[0029] White Peony Root: nourishes blood and regulates menstruation, astringes yin and stops sweating, softens the liver and relieves pain, and calms liver yang.

[0030] Dendrobium: nourishes the stomach and produces body fluid, nourishes yin and eliminates heat, improves eyesight, and strengthens the waist.

[0031] Rehmannia root: nourishes blood and yin, replenishes essence and benefits marrow.

[0032] Astragalus: tonifies Qi and raises Yang, benefits the Wei and consolidates the exterior, removes toxins and promotes tissue regeneration, promotes diuresis and reduces swelling.

[0033] This application combines beautiful pea vine, golden sunflower, and Dendrobium candidum to produce a synergistic effect, which can promote body fluid and moisten dryness, replenish qi and blood, and nourish the lungs. It helps to promote blood circulation, metabolism, and scavenge free radicals, promote collagen regeneration, and enhance skin vitality. It is applied in the matrix of cosmetic formulas, which can replenish qi and blood for middle-aged and elderly women, make their skin ruddy, and make their faces shiny, ensuring good health and beautiful appearance.

[0034] Secondly, the preparation process of the present application adopts the method of mixed extraction of traditional Chinese medicine, weighs traditional Chinese medicine according to the drug formula, and first adds cellulase treatment, which can degrade the plant material to form a more sparse structure, make the effective ingredients easier to release, and can also reduce the viscosity of the material, which is conducive to the subsequent steps; and then ferment with Lactobacillus casei at room temperature to further degrade the macromolecular substances in the plant material into small molecule active substances; and then use ethanol to extract the effective components of traditional Chinese medicine, avoiding the cumbersome and repetitive extraction of traditional Chinese medicine components one by one, and can make each traditional Chinese medicine component fully contact and mix evenly, which is conducive to the final use on the human body. In addition, the difference from the traditional extraction process is that alcohol solvents are introduced, and isopentyl glycol is used to replace ethanol as the solvent of traditional Chinese medicine components, which helps the thorough volatilization of ethanol and eliminates the possibility of ethanol residue. Compared with conventional alcohols, it has a stronger solubility for active substances of medium polarity; and isopentyl glycol has good moisturizing properties, and the water in the mixed extract of traditional Chinese medicine acts on human skin to make people feel more refreshed and improve the use effect.

[0035] Finally, the mixed Chinese medicinal extract for regulating qi and blood of the present invention can be directly applied externally to the human body, and can also be used as other external application products. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for describing the embodiments or the prior art are briefly introduced below.

[0037] Figure 1 The number of white blood cells in each group is shown; Figure 2 The number of red blood cells in each group is shown; Figure 3 The hemoglobin amount of each group is shown; Figure 4 The platelet counts of each group are shown; * indicates P < 0.05 compared with the blank group, ** indicates P < 0.01 compared with the blank group; # indicates P < 0.05 compared with the blank group, and ## indicates P < 0.01 compared with the blank group. DETAILED DESCRIPTION

[0038] The embodiments in the specification are only used to illustrate the present invention and do not limit the scope of protection of the present invention. The scope of protection of the present invention is only limited by the claims, and any omission, replacement or modification made on the basis of the embodiments disclosed in the present invention shall fall within the scope of protection of the present invention.

[0039] In order to deepen the understanding of the present invention, the present invention will be further described in detail below in conjunction with examples. The examples are only used to explain the present invention and do not constitute a limitation on the protection scope of the present invention.

[0040] In order to avoid repetition, the materials and experimental methods used in the verification examples are described uniformly.

[0041] 1. Validation of test animals SPF Kunming mice, 6-8 weeks old, half male and half female, were provided by Guangdong Experimental Animal Center. The mice were raised in an environment with a temperature of 18-25°C and a relative humidity of 50%-70%.

[0042] 2. Verification example basic feed Provided by the Experimental Animal Center of Guangzhou University of Chinese Medicine.

[0043] 3. Validation of test reagents (1) Distilled water.

[0044] (2) Ejiao oral liquid. Produced by Yuanda Pharmaceutical Huangshi Feiyun Pharmaceutical Co., Ltd.

[0045] (3) Mixed extracts of traditional Chinese medicine for regulating qi and blood. Made in the laboratory.

[0046] 4. Verification of instrument The Japanese Sysmex XT-2000iv animal-specific fully automatic blood analyzer, electronic balance (Shanghai Qinghai Instrument Co., Ltd., model FA1104N), 722 spectrophotometer, and high-speed low-temperature centrifuge were provided by the Experimental Center of Guangzhou University of Chinese Medicine.

[0047] 5. Validation Example Experimental Method According to the "Health Food Functionality Evaluation Procedures and Test Methods": (1) Grouping of test animals Kunming mice were randomly divided according to body weight, with half males and half females in each group.

[0048] (2) Determination of liver glycogen The Qi-tonifying effect of a drug on the human body can be reflected by liver glycogen. If the drug has the Qi-tonifying effect, the liver glycogen will increase. Verification Example The Qi-tonifying effect of a mixed extract of Chinese medicine for regulating Qi and blood was tested by a mouse liver glycogen test experiment.

[0049] (3) Determination of white blood cells (WBC), red blood cells (RBC), hemoglobin (HGB) and platelets (PLT) The blood-tonifying effect of a drug on the human body can be reflected by blood component analysis. If a drug has a blood-tonifying effect, it will promote hematopoiesis, and the content of WBC, RBC, HGB and PLT in the blood will increase. Verification example The blood-tonifying effect of a mixed extract of traditional Chinese medicine for regulating qi and blood was tested by a mouse blood analysis test experiment.

[0050] 6. Verification Case Data Processing SPSS10.0 for Windows was used for data statistical analysis. Example 1

[0051] A mixed extract of traditional Chinese medicine for regulating qi and blood is prepared according to the following steps: Step (1): weigh the following traditional Chinese medicine mixed components in parts by weight: 5 parts of Millettia oleifera, 10 parts of Heliotropium chrysantha, and 5 parts of Dendrobium candidum, mix them, and grind them into drug powder; mix the drug powder, water, and cellulase in a weight ratio of 100:600:3, hydrolyze at 50° C. for 5 hours, and inactivate the enzyme in a boiling water bath for 10 minutes to obtain a hydrolyzate; Step (2): mixing the hydrolyzate and Lactobacillus casei, wherein the amount of Lactobacillus casei added is 1% of the weight of the hydrolyzate; and fermenting at room temperature for 2 days to obtain a fermented product; Step (3): adding ethanol to the fermented product until the ethanol concentration reaches 75%, soaking for 1 hour to obtain a Chinese medicine soaking liquid; Step (4): using a condensation reflux device to condense and reflux the Chinese medicine soaking liquid for 3 hours at a heating temperature of 70° C., filtering the condensed refluxed liquid to obtain a Chinese medicine ethanol extract; Step (5): uniformly mixing the Chinese medicine ethanol extract and an equal weight of isoprene glycol to obtain a mixed solution; Step (6): The mixed liquid is subjected to rotary evaporation using a rotary evaporator at a heating temperature of 80° C. until no more liquid in the condenser flows into the receiving bottle. The liquid collected in the evaporating bottle is the mixed extract of traditional Chinese medicine for regulating qi and blood. Example 2

[0052] A mixed extract of traditional Chinese medicine for regulating qi and blood is prepared according to the following steps: Step (1): weigh the following traditional Chinese medicine mixed components in parts by weight: 15 parts of Millettia oleifera, 1 part of Heliotropium chrysantha, and 15 parts of Dendrobium candidum; mix and grind into drug powder; mix the drug powder, water, and cellulase in a weight ratio of 100:600:3, hydrolyze at 55° C. for 3 hours, and inactivate the enzyme in a boiling water bath for 10 minutes to obtain a hydrolyzate; Step (2): mixing the hydrolyzate and Lactobacillus casei, wherein the amount of Lactobacillus casei added is 1% of the weight of the hydrolyzate; and fermenting at room temperature for 4 days to obtain a fermentation product; Step (3): adding ethanol to the fermented product until the ethanol concentration reaches 75%, soaking for 1 hour to obtain a Chinese medicine soaking liquid; Step (4): condense and reflux the Chinese medicine soaking liquid with a condensation reflux device for 2 hours at a heating temperature of 90° C., filter the condensed refluxed liquid to obtain a Chinese medicine ethanol extract; Step (5): uniformly mixing the Chinese medicine ethanol extract and an equal weight of isoprene glycol to obtain a mixed solution; Step (6): The mixed liquid is subjected to rotary evaporation using a rotary evaporator at a heating temperature of 50° C. until no more liquid in the condenser flows into the receiving bottle. The liquid collected in the evaporating bottle is the mixed extract of traditional Chinese medicine for regulating qi and blood. Example 3

[0053] A mixed extract of traditional Chinese medicine for regulating qi and blood is prepared according to the following steps: Step (1): weigh the following traditional Chinese medicine mixed components in parts by weight: 10 parts of Millettia oleifera, 5 parts of Heliotropium chrysantha, and 10 parts of Dendrobium candidum; mix and grind into drug powder; mix the drug powder, water, and cellulase in a weight ratio of 100:600:3, hydrolyze at 52° C. for 4 hours, and inactivate the enzyme in a boiling water bath for 10 minutes to obtain a hydrolyzate; Step (2): mixing the hydrolyzate and Lactobacillus casei, wherein the amount of Lactobacillus casei added is 1% of the weight of the hydrolyzate; and fermenting at room temperature for 3 days to obtain a fermentation product; Step (3): adding ethanol to the fermented product until the ethanol concentration reaches 75%, soaking for 1 hour to obtain a Chinese medicine soaking liquid; Step (4): using a condensation reflux device to condense and reflux the Chinese medicine soaking liquid for 2.5 hours at a heating temperature of 80° C., filtering the condensed refluxed liquid to obtain a Chinese medicine ethanol extract; Step (5): uniformly mixing the Chinese medicine ethanol extract and an equal weight of isoprene glycol to obtain a mixed solution; Step (6): The mixed liquid is subjected to rotary evaporation using a rotary evaporator at a heating temperature of 60° C. until no more liquid in the condenser flows into the receiving bottle. The liquid collected in the evaporating bottle is the mixed extract of traditional Chinese medicine for regulating qi and blood. Example 4

[0054] A mixed extract of traditional Chinese medicine for regulating qi and blood is prepared according to the following steps: Step (1): weigh the following Chinese medicine mixed components in parts by weight: 10 parts of Millettia oleifera, 5 parts of Heliotropium chrysantha, 10 parts of Dendrobium candidum, 8 parts of Ginseng, 8 parts of Poria, 5 parts of Atractylodes macrocephala, 10 parts of Licorice, 7 parts of Chuanxiong, 10 parts of Rehmannia glutinosa, 7 parts of Angelica sinensis, 5 parts of White Peony Root, 5 parts of Codonopsis pilosula, 8 parts of Cinnamon Bark, 7 parts of Dry Ginger, 12 parts of Astragalus and 8 parts of Dendrobium candidum; mix and grind into drug powder; mix the drug powder, water and cellulase in a weight ratio of 100:600:3, hydrolyze at 52° C. for 4 hours, inactivate the enzyme in a boiling water bath for 10 minutes, and obtain a hydrolyzate; Step (2): mixing the hydrolyzate and Lactobacillus casei, wherein the amount of Lactobacillus casei added is 1% of the weight of the hydrolyzate; and fermenting at room temperature for 3 days to obtain a fermentation product; Step (3): adding ethanol to the fermented product until the ethanol concentration reaches 75%, soaking for 1 hour to obtain a Chinese medicine soaking liquid; Step (4): using a condensation reflux device to condense and reflux the Chinese medicine soaking liquid for 2.5 hours at a heating temperature of 80° C., filtering the condensed refluxed liquid to obtain a Chinese medicine ethanol extract; Step (5): uniformly mixing the Chinese medicine ethanol extract and an equal weight of isoprene glycol to obtain a mixed solution; Step (6): The mixed liquid is subjected to rotary evaporation using a rotary evaporator at a heating temperature of 60° C. until no more liquid in the condenser flows into the receiving bottle. The liquid collected in the evaporating bottle is the mixed extract of traditional Chinese medicine for regulating qi and blood. Example 5

[0055] A mixed extract of traditional Chinese medicine for regulating qi and blood is prepared according to the following steps: Step (1): weigh the following Chinese medicine mixed components in parts by weight: 10 parts of Millettia oleifera, 5 parts of Heliotropium chrysantha, 10 parts of Dendrobium candidum, 12 parts of Ginseng, 5 parts of Poria, 8 parts of Atractylodes macrocephala, 7 parts of Licorice, 10 parts of Ligusticum chuanxiong, 7 parts of Rehmannia glutinosa, 10 parts of Angelica sinensis, 3 parts of White Peony Root, 8 parts of Codonopsis pilosula, 5 parts of Cinnamon Bark, 10 parts of Dry Ginger, 8 parts of Astragalus and 12 parts of Dendrobium candidum; mix and grind into drug powder; mix the drug powder, water and cellulase in a weight ratio of 100:600:3, hydrolyze at 52° C. for 4 hours, inactivate the enzyme in a boiling water bath for 10 minutes, and obtain a hydrolyzate; Step (2): mixing the hydrolyzate and Lactobacillus casei, wherein the amount of Lactobacillus casei added is 1% of the weight of the hydrolyzate; and fermenting at room temperature for 3 days to obtain a fermentation product; Step (3): adding ethanol to the fermented product until the ethanol concentration reaches 75%, soaking for 1 hour to obtain a Chinese medicine soaking liquid; Step (4): using a condensation reflux device to condense and reflux the Chinese medicine soaking liquid for 2.5 hours at a heating temperature of 80° C., filtering the condensed refluxed liquid to obtain a Chinese medicine ethanol extract; Step (5): uniformly mixing the Chinese medicine ethanol extract and an equal weight of isoprene glycol to obtain a mixed solution; Step (6): The mixed liquid is subjected to rotary evaporation using a rotary evaporator at a heating temperature of 60° C. until no more liquid in the condenser flows into the receiving bottle. The liquid collected in the evaporating bottle is the mixed extract of traditional Chinese medicine for regulating qi and blood.

[0056] Comparative Example 1 A mixed extract of traditional Chinese medicine for regulating qi and blood is prepared according to the following steps: Step (1): weigh the following parts by weight of the Chinese medicine mixture component: 25 parts of Millettia oleifera; mix and crush into medicine powder; mix the medicine powder, water and cellulase in a weight ratio of 100:600:3, hydrolyze at 52° C. for 4 hours, and inactivate the enzyme in a boiling water bath for 10 minutes to obtain a hydrolyzate; Step (2): mixing the hydrolyzate and Lactobacillus casei, wherein the amount of Lactobacillus casei added is 1% of the weight of the hydrolyzate; and fermenting at room temperature for 3 days to obtain a fermentation product; Step (3): adding ethanol to the fermented product until the ethanol concentration reaches 75%, soaking for 1 hour to obtain a Chinese medicine soaking liquid; Step (4): using a condensation reflux device to condense and reflux the Chinese medicine soaking liquid for 2.5 hours at a heating temperature of 80° C., filtering the condensed refluxed liquid to obtain a Chinese medicine ethanol extract; Step (5): uniformly mixing the Chinese medicine ethanol extract and an equal weight of isoprene glycol to obtain a mixed solution; Step (6): The mixed liquid is subjected to rotary evaporation using a rotary evaporator at a heating temperature of 60° C. until no more liquid in the condenser flows into the receiving bottle. The liquid collected in the evaporating bottle is the mixed extract of traditional Chinese medicine for regulating qi and blood.

[0057] Comparative Example 2 A mixed extract of traditional Chinese medicine for regulating qi and blood is prepared according to the following steps: Step (1): weigh the following parts by weight of the Chinese medicine mixed component: 25 parts of golden sunflower; mix and crush into medicine powder; mix the medicine powder, water and cellulase in a weight ratio of 100:600:3, hydrolyze at 52° C. for 4 hours, and inactivate the enzyme in a boiling water bath for 10 minutes to obtain a hydrolyzate; Step (2): mixing the hydrolyzate and Lactobacillus casei, wherein the amount of Lactobacillus casei added is 1% of the weight of the hydrolyzate; and fermenting at room temperature for 3 days to obtain a fermentation product; Step (3): adding ethanol to the fermented product until the ethanol concentration reaches 75%, soaking for 1 hour to obtain a Chinese medicine soaking liquid; Step (4): using a condensation reflux device to condense and reflux the Chinese medicine soaking liquid for 2.5 hours at a heating temperature of 80° C., filtering the condensed refluxed liquid to obtain a Chinese medicine ethanol extract; Step (5): uniformly mixing the Chinese medicine ethanol extract and an equal weight of isoprene glycol to obtain a mixed solution; Step (6): The mixed liquid is subjected to rotary evaporation using a rotary evaporator at a heating temperature of 60° C. until no more liquid in the condenser flows into the receiving bottle. The liquid collected in the evaporating bottle is the mixed extract of traditional Chinese medicine for regulating qi and blood.

[0058] Comparative Example 3 A mixed extract of traditional Chinese medicine for regulating qi and blood is prepared according to the following steps: Step (1): weigh the following parts by weight of the Chinese medicine mixed component: 25 parts of Dendrobium nobile; mix and crush into medicine powder; mix the medicine powder, water and cellulase in a weight ratio of 100:600:3, hydrolyze at 52° C. for 4 hours, and inactivate the enzyme in a boiling water bath for 10 minutes to obtain a hydrolyzate; Step (2): mixing the hydrolyzate and Lactobacillus casei, wherein the amount of Lactobacillus casei added is 1% of the weight of the hydrolyzate; and fermenting at room temperature for 3 days to obtain a fermentation product; Step (3): adding ethanol to the fermented product until the ethanol concentration reaches 75%, soaking for 1 hour to obtain a Chinese medicine soaking liquid; Step (4): using a condensation reflux device to condense and reflux the Chinese medicine soaking liquid for 2.5 hours at a heating temperature of 80° C., filtering the condensed refluxed liquid to obtain a Chinese medicine ethanol extract; Step (5): uniformly mixing the Chinese medicine ethanol extract and an equal weight of isoprene glycol to obtain a mixed solution; Step (6): The mixed liquid is subjected to rotary evaporation using a rotary evaporator at a heating temperature of 60° C. until no more liquid in the condenser flows into the receiving bottle. The liquid collected in the evaporating bottle is the mixed extract of traditional Chinese medicine for regulating qi and blood.

[0059] Comparative Example 4 A mixed extract of traditional Chinese medicine for regulating qi and blood is prepared according to the following steps: Step (1): weigh the following traditional Chinese medicine mixed components in parts by weight: 10 parts of Millettia oleifera, 5 parts of Heliotropium chrysantha, and 10 parts of Dendrobium candidum; mix and grind into drug powder; mix the drug powder, water, and cellulase in a weight ratio of 100:600:3, hydrolyze at 52° C. for 4 hours, and inactivate the enzyme in a boiling water bath for 10 minutes to obtain a hydrolyzate; Step (2): mixing the hydrolyzate and Lactobacillus casei, wherein the amount of Lactobacillus casei added is 1% of the weight of the hydrolyzate; and fermenting at room temperature for 3 days to obtain a fermentation product; Step (3): adding ethanol to the fermented product until the ethanol concentration reaches 75%, soaking for 1 hour to obtain a Chinese medicine soaking liquid; Step (4): using a condensation reflux device to condense and reflux the Chinese medicine soaking liquid for 2.5 hours at a heating temperature of 80° C., filtering the condensed refluxed liquid to obtain a Chinese medicine ethanol extract; Step (5): uniformly mixing the Chinese medicine ethanol extract and an equal weight of glycerol to obtain a mixed solution; Step (6): The mixed liquid is subjected to rotary evaporation using a rotary evaporator at a heating temperature of 60° C. until no more liquid in the condenser flows into the receiving bottle. The liquid collected in the evaporating bottle is the mixed extract of traditional Chinese medicine for regulating qi and blood.

[0060] Comparative Example 5 A mixed extract of traditional Chinese medicine for regulating qi and blood is prepared according to the following steps: Step (1): weigh the following traditional Chinese medicine mixed components in parts by weight: 10 parts of Millettia oleifera, 5 parts of Heliotropium chrysantha, and 10 parts of Dendrobium nobile; mix and grind into medicine powder; Step (2): soaking the drug powder in a mixed solution of 50 parts by weight of water and 50 parts by weight of isopentyl glycol for 1 hour, then heating to 100° C. and boiling for 1 hour; centrifuging and taking the supernatant to obtain a mixed extract of Chinese medicine for regulating qi and blood.

[0061] Verification example: Qi and blood-tonifying effect of mixed extracts of traditional Chinese medicines (1) The Qi-tonifying effect of a drug on the human body can be reflected by liver glycogen. If a drug has a Qi-tonifying effect, liver glycogen will increase. This verification example tests the Qi-tonifying effect of a mixed extract of traditional Chinese medicine for regulating Qi and blood through a mouse liver glycogen test.

[0062] 96 Kunming mice, half male and half female, were randomly divided into 12 groups according to body weight, with half male and half female in each group; the test animals were fed 10g of feed every day, and 2 hours later, the test animals were gavaged with drugs for 14 days. 30 minutes after feeding the drugs on the 14th day, the eyeballs of the mice were removed to collect blood, and after being placed at room temperature for 30 minutes, the blood was centrifuged at 3000r / min and 40℃ for 20-30 minutes to separate the serum, and the liver glycogen content of each group of mice was measured according to the Elisa kit.

[0063] The following are the grouping conditions and the intragastric medicaments and their dosages. The mixed extracts of Chinese medicines for regulating qi and blood used are the mixed extracts of Chinese medicines for regulating qi and blood prepared in Examples 1-5 and Comparative Examples 1-5, respectively. A blank control group was set up: 0.4 ml / day of distilled water; a positive control group: 0.4 ml / day of donkey-hide gelatin oral solution.

[0064] Table 1 Effects of different drugs on the body weight of mice

[0065] Results: As shown in Table 1, after 14 days of the test, each group did not cause excessive weight gain, and the average body weight of the example was slightly higher than that of the control example.

[0066] Table 2 Effects of mixed extracts of traditional Chinese medicine for regulating qi and blood on liver glycogen content in mice

[0067] As shown in Table 2, the mixed Chinese herbal extract prepared in the embodiment of the present invention can significantly increase the liver glycogen content of mice (p < 0.01), and the effect is better than that of donkey-hide gelatin oral liquid, reflecting a good qi-invigorating effect.

[0068] (2) The blood-tonifying effect of a drug on the human body can be reflected by blood component analysis. If a drug has a blood-tonifying effect, it will promote hematopoiesis, and the content of white blood cells (WBC), red blood cells (RBC), hemoglobin (HGB) and platelets (PLT) in the blood will increase. This verification example tests the blood-tonifying effect of a mixed extract of traditional Chinese medicine for regulating qi and blood through a mouse blood analysis test experiment.

[0069] 40 Kunming mice, half male and half female, were randomly divided into 5 groups according to body weight, with half male and half female in each group; the animals were fed half of their feed every day, and 2 hours after feeding 5g of feed, the animals were injected with drugs at a body weight of 0.01mL / (g·day); in addition, except for the blank group, the animals in each group were subcutaneously injected with acetylphenylhydrazine (APH) in saline solution of 0.2g / kg, 0.1g / kg, and 0.1g / kg on the 1st, 4th, and 7th days of the experiment; the duration was 14 days. 30 minutes after feeding the drug on the 14th day, the eyeballs of the mice were removed and blood was collected. After being placed at room temperature for 30 minutes, the eyeballs were blooded and placed in centrifuge tubes. The biochemical indexes were detected and analyzed using the Japanese SysmexXT-2000iv animal-specific fully automatic blood analyzer. The detection indicators were WBC, RBC, HGB and PLT.

[0070] The following are the grouping conditions, injection drugs and their dosages. The mixed Chinese herbal extract for regulating qi and blood used is the mixed Chinese herbal extract for regulating qi and blood of Example 5.

[0071] A. Blank control group (Control): distilled water 0.4 ml / day; B. Positive control group (Positive): 0.4 ml / day of donkey-hide gelatin oral solution; C. Low-dose group of mixed extracts of traditional Chinese medicines for regulating qi and blood (Low): 0.2 ml / day of mixed extracts of traditional Chinese medicines for regulating qi and blood; D. High-dose group of mixed extract of traditional Chinese medicine for regulating qi and blood (High): 0.4 ml / day of mixed extract of traditional Chinese medicine for regulating qi and blood.

[0072] Results: Figure 1-4 As shown, the mixed Chinese herbal extract prepared in the embodiment of the present invention can significantly increase the WBC, RBC, HGB and PLT contents in the blood of mice, reflecting a good blood-tonifying effect. Example 6

[0073] An anti-aging cream is composed of the following components in percentage by weight: 5% cyclopentasiloxane, 2% polydimethylsiloxane, 3% cetearyl alcohol, 2% cetearyl glucoside, 1% methyl glucose sesquistearate, 5% glycerol, 3% butylene glycol, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, 0.4% carbomer, 0.4% arginine, 5% of the mixed extract of traditional Chinese medicines for regulating qi and blood of Example 5, and the balance water.

[0074] 30 volunteers were recruited to try the anti-aging cream prepared in Example 6 of the present invention. The selection criteria for the volunteers were: women aged 40-55, insufficient qi and blood, and their skin was rough, pale, and dull. The volunteers took an appropriate amount of anti-aging cream on their faces, gently massaged and applied evenly, used it once in the morning and evening, and conducted a return visit after continuous use for 3 months. The return visit found that 18 volunteers had achieved significant results, and their skin became ruddy and shiny, and 10 volunteers had achieved effective results, and their skin pale and rough symptoms were alleviated to a certain extent. In general, in this volunteer trial test, the anti-aging cream prepared in the embodiment of the present invention can effectively replenish qi and blood, replenish qi and blood, make the skin ruddy, and make the face shiny, ensuring good health and beautiful appearance.

[0075] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0076] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for those of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A mixed extract of traditional Chinese medicine for regulating qi and blood, characterized in that: Its ingredients include mixed components of traditional Chinese medicine; The traditional Chinese medicine mixed components mainly include: 5-15 parts of Millettia chinensis, 1-10 parts of Helichrysum chrysantha, and 5-15 parts of Dendrobium nobile.

2. The mixed Chinese medicinal extract for regulating qi and blood according to claim 1, characterized in that: The traditional Chinese medicine mixed components also include: 8-12 parts of ginseng, 5-8 parts of Poria cocos, 5-8 parts of Atractylodes macrocephala, 7-10 parts of licorice, 7-10 parts of Chuanxiong, 7-10 parts of Rehmannia root, 7-10 parts of Angelica sinensis, 3-5 parts of white peony root, 5-8 parts of Codonopsis pilosula, 5-8 parts of cinnamon bark, 7-10 parts of dried ginger, 8-12 parts of Astragalus and 8-12 parts of Dendrobium candidum.

3. The mixed Chinese medicinal extract for regulating qi and blood according to claim 1 or 2, characterized in that: The preparation method of the mixed extract of traditional Chinese medicine for regulating qi and blood comprises the following preparation steps: Step (1): weighing the Chinese medicine mixed components according to the number of portions, mixing, and crushing into drug powder; mixing the drug powder, water, and cellulase, hydrolyzing at 50-55° C. for 3-5 hours, and inactivating the enzyme in a boiling water bath for 10 minutes to obtain a hydrolyzate; Step (2): mixing the hydrolyzate and Lactobacillus casei, and fermenting at room temperature for 2-4 days to obtain a fermentation product; Step (3): adding ethanol to the fermented product until the ethanol volume concentration reaches 75%, soaking for more than 0.5 hours to obtain a Chinese medicine soaking liquid; Step (4): using a condensation reflux device to condense and reflux the Chinese medicine soaking liquid for 2-3 hours, filtering the condensed refluxed object to remove the drug residue, and obtaining the Chinese medicine ethanol extract; Step (5): uniformly mixing the Chinese medicine ethanol extract and the alcohol solvent in a mass ratio of 1:1 to obtain a mixed solution; Step (6): The mixed liquid is subjected to rotary evaporation using a rotary evaporator until no more liquid in the condenser flows into the receiving bottle, and the liquid remaining in the evaporation bottle is the mixed extract of Chinese medicine for regulating qi and blood, which contains water, alcohol solvent and mixed components of Chinese medicine.

4. The mixed Chinese medicinal extract for regulating qi and blood according to claim 3, characterized in that: The drug powder, water and cellulase are mixed in a weight ratio of 100:600:

3.

5. The mixed Chinese medicinal extract for regulating qi and blood according to claim 3, characterized in that: The added amount of the Lactobacillus casei is 1% of the weight of the hydrolyzate.

6. The mixed Chinese medicinal extract for regulating qi and blood according to claim 3, characterized in that: The heating temperature used in the condensation reflux process is 70-90°C.

7. The mixed Chinese medicinal extract for regulating qi and blood according to claim 3, characterized in that: The pore size of the filter membrane used for filtration is 200-600 meshes.

8. The mixed Chinese medicinal extract for regulating qi and blood according to claim 3, characterized in that: The alcohol solvent is isoprene glycol.

9. The mixed Chinese medicinal extract for regulating qi and blood according to claim 3, characterized in that: The heating temperature used in the rotary evaporation process is 50-80° C., and the vacuum degree is 0.05-0.1 MPa.

10. Use of the mixed extract of traditional Chinese medicines for regulating qi and blood according to any one of claims 1 to 9 in the preparation of cosmetics.

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