Composition with hypoglycemic effect

Abstract

The invention relates to a composition with a hypoglycemic effect, and belongs to the technical field of traditional Chinese medicines. The composition with the hypoglycemic effect is prepared from the following components in parts by weight: 1 part of radix ophiopogonis, 1 part of corn stigma, 2 parts of poria cocos in Yunnan, 2 parts of Pu'er tea old yellow tablets, 2 parts of Pu'er raw tea and 2 parts of folium mori. The preparation method of the composition with the hypoglycemic effect comprises the following steps: (1) weighing: weighing the raw materials in parts by weight; (2) adding radix ophiopogonis, corn stigma and poria cocos into water of which the weight is 8 times that of the radix ophiopogonis, corn stigma and poria cocos, decocting for 1.5-2 hours, adding Pu'er tea and folium mori, and decocting for 0.5-1 hour; (3) filtering the obtained filtrate through a 200-mesh sieve, concentrating the filtrate into paste, and drying the paste to obtain an extract; and (4) crushing the obtained extract, and packaging to obtain the composition with the hypoglycemic effect. The composition is wide in raw material source, simple in preparation method and low in cost; the prepared composition can play a better role in reducing blood sugar.

Description

Technical Field

[0001] The present application relates to the technical field of traditional Chinese medicine, and in particular to a composition having a blood sugar lowering effect. Background Art

[0002] With the improvement of living standards and unreasonable diet, insufficient sleep and rest, irregular living and work schedules have become common phenomena. Some people are overly nervous and stressed, which causes endocrine disorders. Especially for white-collar workers, lack of exercise and physical overdraft, blood sugar in the body is retained for a long time, and finally forms hyperglycemia. Over time, it becomes an "invisible killer" that endangers health and even becomes an irreversible lifelong disease.

[0003] Diabetes is a common endocrine metabolic disease with a certain genetic tendency, with many complications and long treatment time. At present, the conventional treatment for diabetes is mainly oral hypoglycemic Western medicine. Mainly sulfonylureas, biguanides, a-glucosidase inhibitors, new insulin sensitizers, etc. Although these drugs help diabetics control blood sugar levels with different mechanisms of action, these drugs have side effects such as hypoglycemia and gastrointestinal reactions. At the same time, as the treatment time increases, the hypoglycemic effect tends to decrease. According to traditional Chinese medicine theory, diabetes belongs to the category of thirst. Traditional Chinese medicine has the characteristics of mild and lasting therapeutic effects, fewer side effects, and can be taken for a long time in the treatment of diabetes. Its mechanism of action is often a comprehensive effect of multiple targets, multiple effects, and multiple functions. It has irreplaceable advantages over Western medicine, and is therefore chosen by more diabetic patients.

[0004] The announcement number CN114848748B, "A composition with hypoglycemic effect and its preparation method", is a composition with hypoglycemic effect and its preparation method, the composition is composed of the following components in weight proportion: 3-7 parts of fenugreek, 1-7 parts of cyclocarya paliurus, 1-6 parts of mulberry leaves, 2-8 parts of astragalus, 2-8 parts of ophiopogon, 1-6 parts of Pu'er tea, and 1-6 parts of mannitol. The present invention gives full play to the advantages of traditional Chinese medicine resources, under the guidance of traditional Chinese medicine theory, selects fenugreek, cyclocarya paliurus leaves, mulberry leaves, astragalus, ophiopogon, and Pu'er tea as raw materials, and makes a modern and traditional combination of hypoglycemic products through reasonable preparation and modern technology. The present invention has clear functions, controllable quality, safe to take, and can be taken for a long time.

[0005] Although the above-mentioned composition with hypoglycemic effect has achieved a good hypoglycemic effect, the preparation of the composition involves many raw materials, involving as many as seven components, and the formula is not simple enough; further, in the method for preparing the composition with hypoglycemic effect in the above-mentioned patent, when processing Pu'er tea, it is necessary to use 60Co-γ rays to irradiate 1 / 3 of the formula amount of Pu'er tea and then grind it twice to prepare Pu'er tea powder. The irradiation treatment and two grinds make the entire composition preparation method cumbersome and not simple enough; further, in this method, when extracting and concentrating the remaining raw materials, it is necessary to conduct a secondary concentration, filter after the first concentration and then conduct a secondary concentration. This process further makes the entire preparation method lengthy and not simple enough.

[0006] Therefore, how to simplify the composition of the formula raw materials while ensuring the blood sugar reduction effect and shorten the preparation process has always been a constant pursuit of those skilled in the art. Summary of the invention

[0007] In order to solve or partially solve the problems existing in the related art, the present application provides a composition with hypoglycemic effect, which has a simpler formula and is composed of Ophiopogon japonicus, Corn Silk, Poria cocos, Pu'er Tea Old Yellow Tablets, Pu'er Raw Tea, and mulberry leaves. The raw materials are widely available, and the preparation method is simple and the cost is low. At the same time, the composition in the present application is compared with metformin, a conventional drug with obvious hypoglycemic effect, and the efficacy is even slightly better than metformin, indicating that the composition in the present application can achieve a better hypoglycemic effect.

[0008] The present application discloses a composition having a hypoglycemic effect, which is composed of the following ingredients in proportion by weight: 1 part of Ophiopogon japonicus, 1 part of Corn Silk, 2 parts of Yun Fuling, 2 parts of Pu'er Tea Old Yellow Tablets, 2 parts of Pu'er Raw Tea, and 2 parts of Mulberry Leaves;

[0009] The preparation method of the composition having the hypoglycemic effect comprises the following steps:

[0010] (1) Weighing: Weigh ophiopogon japonicus, corn silk, Yunfuling, Pu'er tea old yellow slices, Pu'er raw tea, and mulberry leaves according to the weight of each raw material;

[0011] (2) Add 8 times the weight of water to Radix Ophiopogonis, Corn Silk, and Poria Cocos, and boil for 1.5 hours, then add Pu'er Tea Old Yellow Pieces, Pu'er Raw Tea, and Mulberry Leaves, and boil for another 0.5 hours;

[0012] (3) The filtrate is passed through a 200-mesh sieve and concentrated to a paste, and then dried to obtain an extract;

[0013] (4) The obtained extract is crushed and packaged to obtain a composition having a blood sugar lowering effect.

[0014] Furthermore, the specific operation of concentrating the filtrate through a 200-mesh sieve to a paste is: passing the filtrate after decoction through a 200-mesh sieve, and then concentrating under reduced pressure. When the filtrate is concentrated under reduced pressure to 50° C., the relative density is measured to be 1.20-1.25.

[0015] Furthermore, the concentration temperature of the reduced pressure concentration is 70° C. and the vacuum degree is -0.06Mpa.

[0016] Furthermore, the drying temperature in step (3) is 70-80°C.

[0017] Furthermore, after the extract obtained in step (4) is crushed, the powder is packaged, and 0.5 g is packed in each bag for inner packaging and 10 bags / bag for outer packaging.

[0018] It should be noted that the old yellow flakes involved in this application refer to the tea leaves that are screened out according to production standards during the raw material screening process because the tea leaves are loose and thick, and the grade of the tea leaves is low. They are commonly called old yellow flakes. The formation of old yellow flakes is that, first, the third leaf other than one bud and one leaf or one bud and two leaves when picking, generally does not reach the fourth leaf; second, the tea leaves are not rolled in place during rolling, and the tea leaves are relatively loose; in these two cases, killing the green leaves will cause the leaves to turn yellow or darker green.

[0019] Pu'er raw tea refers to a general term for various Yunnan teas (cake tea, brick tea, Tuo tea, dragon ball tea) that are made from Yunnan large-leaf sun-dried green tea, aged in a natural way, without artificial "fermentation" or "piling" treatments, but are processed and modified in shape.

[0020] In the present invention, the raw materials are selected from Pu'er tea old yellow slices as raw materials, which can not only play a better hypoglycemic effect, but also turn waste into treasure, reduce production costs, and facilitate industrial production and promotion and application. The experimental results show that the composition obtained by measuring the total saponin content of Ophiopogon japonicus and the α-glucosidase inhibition rate of Ophiopogon japonicus through a specific ratio and a specific preparation method is significantly better than other prescriptions and other ratios, and the raw materials play a synergistic effect, which has a good hypoglycemic effect. The specific ratio of the prescription and preparation method in this application has achieved unexpected technical effects.

[0021] The beneficial effects of this application are:

[0022] 1. The composition with hypoglycemic effect in the present application has a simpler formula, and is composed of six raw materials, namely, Radix Ophiopogonis, Stigma Corni, Poria Cocos, Old Yellow Tablets of Pu'er Tea, Pu'er Raw Tea, and Mulberry Leaves. The raw materials are widely available, and the preparation method is simple and the cost is low. At the same time, the composition in the present application is compared with the conventional drug metformin with obvious hypoglycemic effect, and the efficacy is even slightly better than metformin. This shows that the composition in the present application can play a good hypoglycemic effect. DETAILED DESCRIPTION

[0023] The embodiments of the present application will be described in more detail below with reference to examples. Although the embodiments of the present application are shown in the examples, it should be understood that the present application can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to make the present application more thorough and complete, and the scope of the present application can be fully communicated to those skilled in the art.

[0024] Example 1

[0025] A composition with blood sugar lowering effect is composed of the following ingredients in proportion by weight: 1 part of ophiopogon japonicus, 1 part of corn silk, 2 parts of Yunfuling, 2 parts of Pu'er tea Laohuang slices, 2 parts of Pu'er raw tea, and 2 parts of mulberry leaves.

[0026] The preparation method is as follows:

[0027] (1) Weighing: Weigh ophiopogon japonicus, corn silk, Yunfuling, Pu'er tea old yellow slices, Pu'er raw tea, and mulberry leaves according to the weight of each raw material;

[0028] (2) Add 8 times the weight of water to Radix Ophiopogonis, Corn Silk, and Poria Cocos, and boil for 2 hours, then add Pu'er Tea Old Yellow Pieces, Pu'er Raw Tea, and Mulberry Leaves, and boil for another hour;

[0029] (3) The filtrate obtained by decoction is passed through a 200-mesh sieve and concentrated under reduced pressure to 50° C. The relative density is measured to be 1.20 to 1.25. The temperature for the reduced pressure concentration is 70° C. and the vacuum degree is -0.06 Mpa;

[0030] Drying the obtained concentrated extract at a temperature of 70-80° C. for 6-8 hours until the water content is less than 8%, thereby obtaining an extract;

[0031] (4) After the extract is crushed, the powder is packaged, with 0.5 g in each inner bag and 10 bags in each outer bag, and the composition with hypoglycemic effect is obtained by packaging.

[0032] Example 2

[0033] A composition with blood sugar lowering effect is composed of the following ingredients in proportion by weight: 1 part of ophiopogon japonicus, 1 part of corn silk, 2 parts of Yunfuling, 2 parts of Pu'er tea Laohuang slices, 2 parts of Pu'er raw tea, and 2 parts of mulberry leaves.

[0034] The preparation method is as follows:

[0035] (1) Weighing: Weigh ophiopogon japonicus, corn silk, Yunfuling, Pu'er tea old yellow slices, Pu'er raw tea, and mulberry leaves according to the weight of each raw material;

[0036] (2) Add 8 times the weight of water to Radix Ophiopogonis, Corn Silk, and Poria Cocos, and boil for 1.5 hours, then add Pu'er Tea Old Yellow Pieces, Pu'er Raw Tea, and Mulberry Leaves, and boil for another 0.5 hours;

[0037] (3) The filtrate obtained by decoction is passed through a 200-mesh sieve and concentrated under reduced pressure to 50° C. The relative density is measured to be 1.20 to 1.25. The temperature for the reduced pressure concentration is 70° C. and the vacuum degree is -0.06 Mpa;

[0038] Drying the obtained concentrated extract at a temperature of 70-80° C. for 6-8 hours until the water content is less than 8%, thereby obtaining an extract;

[0039] (5) After the extract is crushed, the powder is packaged, with 0.5 g in each inner bag and 10 bags in each outer bag, and the composition with hypoglycemic effect is obtained by packaging.

[0040] Comparative Example 1

[0041] The raw materials for preparing the composition in Comparative Example 1 are composed of the following ingredients in a weight ratio: Ophiopogon japonicus, Poria cocos, Pu'er raw tea, mulberry leaves, and corn silk = 1:2:2:2:1, without Pu'er tea old yellow slices; the rest of the preparation method is the same as Example 1.

[0042] Comparative Example 2

[0043] The raw materials for preparing the composition in Comparative Example 2 are composed of the following ingredients in a weight ratio: Ophiopogon japonicus, Poria cocos, Pu'er tea old yellow slices, mulberry leaves, corn silk = 1:2:2:2:1, without Pu'er raw tea; the rest of the preparation method is the same as Example 1.

[0044] Comparative Example 3

[0045] The raw materials for preparing the composition in Comparative Example 3 are composed of the following ingredients in a weight ratio: Ophiopogon japonicus, Poria cocos, Corn silk, Mulberry leaf = 1:2:1:1, without Pu'er tea Laohuang tablets and Pu'er raw tea; the rest of the preparation method is the same as Example 1.

[0046] Comparative Example 4

[0047] The raw materials for preparing the composition in Comparative Example 4 are composed of the following ingredients in a weight ratio: Ophiopogon japonicus, Poria cocos, Pu'er tea old yellow slices, Pu'er raw tea, mulberry leaves, corn silk = 1:1:1:1:1:1; the rest of the preparation method is the same as Example 1.

[0048] Comparative Example 5

[0049] The raw materials for preparing the composition in Comparative Example 5 are composed of the following ingredients in a weight ratio: Ophiopogon japonicus, Poria cocos, Pu'er tea old yellow slices, Pu'er raw tea, mulberry leaves, and corn silk = 1:2:2:2:2:1; the rest of the preparation method is the same as Example 1.

[0050] Comparative Example 6

[0051] The raw materials for preparing the composition in Comparative Example 6 are composed of the following ingredients in a weight ratio: Ophiopogon japonicus, Poria cocos, Pu'er tea old yellow slices, Pu'er raw tea, mulberry leaves, and corn silk = 2:2:2:1:1:1; the rest of the preparation method is the same as Example 1.

[0052] Comparative Example 7

[0053] The raw materials for preparing the composition in Comparative Example 7 are composed of the following ingredients in a weight ratio: Ophiopogon japonicus, Poria cocos, Pu'er tea old yellow slices, Pu'er raw tea, mulberry leaves, and corn silk = 2:2:2:2:2:1; the rest of the preparation method is the same as Example 1.

[0054] Comparative Example 8

[0055] The raw materials for preparing the composition in Comparative Example 8 are composed of the following ingredients in a weight ratio: Ophiopogon japonicus, Poria cocos, Pu'er tea old yellow slices, Pu'er raw tea, mulberry leaves, corn silk = 3:3:3:3:3:1; the rest of the preparation method is the same as Example 1.

[0056] Comparative Example 9

[0057] The raw materials for preparing the composition in Comparative Example 9 are composed of the following ingredients in a weight ratio: Ophiopogon japonicus, Poria cocos, Pu'er tea old yellow slices, Pu'er raw tea, mulberry leaves, corn silk = 1:3:3:3:3:1; the rest of the preparation method is the same as Example 1.

[0058] Comparative Example 10

[0059] The composition in this comparative example 10 is composed of the following ingredients in proportion by weight: 1 part of Ophiopogon japonicus, 1 part of Corn Silk, 2 parts of Yun Fuling, 2 parts of Pu'er Tea Laohuang Tablets, 2 parts of Pu'er Raw Tea, and 2 parts of Mulberry Leaves.

[0060] The preparation method is as follows:

[0061] (1) Weighing: Weigh ophiopogon japonicus, corn silk, Yunfuling, Pu'er tea old yellow slices, Pu'er raw tea, and mulberry leaves according to the weight of each raw material;

[0062] (2) Add 8 times the weight of water to Radix Ophiopogonis, corn silk, Poria cocos, Pu'er tea old yellow slices, Pu'er raw tea, and mulberry leaves, and boil for 2.0 hours;

[0063] (3) The filtrate obtained by decoction is passed through a 200-mesh sieve and concentrated under reduced pressure to 50° C. The relative density is measured to be 1.20 to 1.25. The temperature for the reduced pressure concentration is 70° C. and the vacuum degree is -0.06 Mpa;

[0064] Drying the obtained concentrated extract at a temperature of 70-80° C. for 6-8 hours until the water content is less than 8%, thereby obtaining an extract;

[0065] (4) After the extract is crushed, the powder is packaged, with 0.5 g per bag for inner packaging and 10 bags per bag for outer packaging, and the composition is obtained by packaging.

[0066] The mouthfeel, total saponins, total flavonoids, tea polyphenols, DPPH scavenging rate, α-glucosidase inhibition rate and α-amylase inhibition rate of the compositions obtained in Example 1 and Comparative Example 1 were measured; the results are shown in Table 1.

[0067] The sensory evaluation of the composition refers to GB / T 14487-2008 "Tea Sensory Evaluation Terminology" and makes appropriate modifications. Seven sensory evaluators are selected to evaluate the dried Dendrobium officinale tea samples. Before the evaluation, the evaluation requirements are explained first, and the products are numbered and asked to taste and evaluate. The evaluators are required to take it seriously during the evaluation. After each evaluation, they must rinse their mouths with water and wait for 5 minutes before evaluating the next numbered product. The weighted average of the soup color, aroma, and taste is taken as the total score of the sample. The sensory evaluation standards are shown in Table 2.

[0068] Table 1 Evaluation table of compositions obtained from different embodiments and comparative examples

[0069]

[0070] Table 2 Sensory evaluation standards

[0071]

[0072] It can be seen from the data in Table 1 that the content of total saponins of Ophiopogon japonicus in the compositions obtained in Examples 1 and 2 of the present application is significantly higher than that in other groups, and those skilled in the art are aware that there are also public reports in the prior art that total saponins of Ophiopogon japonicus have a significant hypoglycemic effect on experimental diabetic mice; at the same time, the α-glucosidase inhibition rate in Examples 1 and 2 is also significantly higher than that in other groups, which also reflects that the composition prepared in this example has a better hypoglycemic effect.

[0073] Furthermore, in order to verify the hypoglycemic effect of the composition obtained in the present application, the following animal function test was conducted. In addition, it should be noted that the composition with hypoglycemic effect obtained in Example 1 is named Dongling Tea Preparation.

[0074] This part of the experiment used a diabetic mouse model caused by a high-fat and high-sugar diet and a type 2 diabetes gene-deficient mouse model. The positive drugs used were metformin and Tasly tea powder (labeled to have blood sugar-lowering effects). The number of high-fat and high-sugar diabetic mice in each group was no less than 9. The number of gene-deficient mice in each group was initially set to 8, except for the low-dose group, which had 7 mice. Later, the low-dose group lost 2 mice during the treatment process, so except for the low-dose group, which had 5 mice, the other groups had 8 mice. This experiment was completed at the Pu'er Tea Research Institute.

[0075] 1. Evaluation of the effect of Dongling tea preparation on hypoglycemic and stabilizing blood sugar in common mice

[0076] 1.1 Experimental Materials

[0077] Male Kunming mice (80 mice), db / dbT2DM (60 mice), normal saline, the composition with hypoglycemic effect prepared in Example 1: Dongling tea preparation (the composition prepared in Example 1), metformin (brand model: Yuedaning National Medicine Standard No. H20051289, Tasly Pu'er Tea (brand model: Diboer, Instant Tea), high-fat feed and ordinary feed, glucose.

[0078] 1.2 Group feeding and drug treatment of mice

[0079] 1.2.1 Diabetic mouse modeling: After the mice were adaptively fed for one week, they were divided into a normal group (15 mice) and a model group (65 mice). The mice in the normal group were fed with ordinary feed, and the mice in the model group were fed with high-fat feed. Body weight and blood sugar were measured once a week until the blood sugar of the model group was significantly higher than that of the normal group, and the modeling was completed.

[0080] 1.2.2 Evaluation of hypoglycemic effect of Dongling tea preparation in mice

[0081] (1) Evaluation of hypoglycemic effect in mice induced by high-fat and high-sugar diet

[0082] The mice were divided into a normal group (12 mice), a model group (12 mice), a positive drug control group (14 mice), a high-dose group (9 mice), a medium-dose group (12 mice), a low-dose group (13 mice), and a free drinking group (12 mice). The latter 6 groups were randomly divided on the premise that there was no significant difference in blood sugar among the mice. The normal group was given ordinary feed + intragastric administration of normal saline; the model group was given high-fat feed + normal saline; the positive drug control group was given high-fat feed + intragastric administration of metformin (50 mg / kg); the high-dose group was given high-fat feed + daily intragastric administration (50 mg / kg) of Dongling tea preparation; the medium-dose group was given high-fat feed + daily intragastric administration (20 mg / kg) of Dongling tea preparation, and the low-dose group was given high-fat feed + daily intragastric administration (10 mg / kg) of Dongling tea preparation; the free drinking group was given high-fat feed + free drinking (50 mg / kg) of Dongling tea preparation. During the administration period, the fasting blood glucose FBG and body weight changes were detected every 7 days. The treatment lasted for 7 weeks. Next, a glucose stabilization experiment was performed.

[0083] (2) Evaluation of hypoglycemic effect in db / dbT2DM gene-deficient mice

[0084] Genetic mice were raised normally until all mice became ill (blood sugar was higher than 7.0), and then drug treatment was started. The mice were divided into model group, positive drug group (Tasly Pu'er Tea), high, medium and low doses. The positive drug control group was gavaged with Tasly Pu'er Tea (50 mg / kg), and the rest of the treatment was the same as the drug treatment of high-fat and high-sugar model mice.

[0085] 1.2.3 Evaluation of the glucose-stabilizing effect of Dongling tea preparation in mice

[0086] Mice were grouped according to 1.2.2, and the glucose stabilization experiment lasted for 6 weeks. In the 6th week, oral glucose tolerance, insulin FINS, glycosylated hemoglobin A1c (GHBA1c) were measured, and pancreatic sections were made.

[0087] 1.2.4 Index detection:

[0088] (1) Fasting blood glucose test method: Change the litter at 8 pm and fast the animals, but allow free access to water, until 8 am the next day, fast for 12 hours. Cut the tail to collect blood, and use a blood glucose meter and test strips to test blood glucose.

[0089] (2) After the fourth week of administration, all mice were fasted but provided with normal water overnight. In the morning of the next day, 2 g / kg of glucose was gavage-administered 1 h after administration. Blood glucose was measured with a blood glucose meter at 0 (before glucose administration), 15, 30, 60, and 120 min later. The glucose tolerance at each time point was compared, and the area under the glucose tolerance curve (AUC) was calculated: AUC = 1 / 4 (blood glucose value at 0 min) + 1 / 2 (blood glucose value at 30 min) + 3 / 4 (blood glucose value at 60 min) + 1 / 2 (blood glucose value at 120 min).

[0090] (3) Determination of glycosylated blood glucose protein and insulin: Three mice were killed in each group, and blood was collected from the orbital venous plexus and sent to Pu'er Traditional Chinese Medicine Hospital for testing.

[0091] 2. Results and Analysis

[0092] 2.1 Background of mice

[0093] The mice used in the experiment were two-month-old male mice, with a body weight range of 30-35g and blood sugar values ​​ranging from 4-7mmol / L. They were active and struggled violently after being caught. They were slender in body shape, had a good appetite, and had normal water intake, and were healthy mice.

[0094] 2.2 Analysis of diabetic mouse modeling results

[0095] 2.2.1 Analysis of mouse body weight results

[0096] After the mice were fed adaptively for 1 week, the model was established. The time was from June 18 to August 12, a total of 7 weeks. The weight of the mice continued to increase over time, and there was no significant difference in weight between the groups, and the change trend was basically the same.

[0097] Table 2-1 Statistics of weight changes of mice in each group during modeling

[0098]

[0099] 2.2.2 Analysis of blood glucose changes in mice

[0100] In the third week after modeling, fasting blood sugar was measured. In the third week, the blood sugar of mice in each group was less than 7, which was within the normal blood sugar range. In the fourth week, the blood sugar of mice in each group showed a more obvious upward trend. Except for the normal group, the blood sugar of the other groups was high, among which the model group was the highest at 9.2mmol / L. In the fifth week, the blood sugar of mice in each group showed a downward trend. Except for the medium-dose group, the other groups basically returned to the normal blood sugar range, among which the lowest blood sugar in the metformin group was 5.7mmol / L. In the sixth week, the medium and low doses continued to decline, and the drinking group basically maintained a balance, but the upward trend of the metformin group was more obvious, and the blood sugar was slightly higher. In the seventh week, the modeling groups were basically consistent, with high blood sugar, and the blood sugar of the normal group was 6.5, which was within the normal range, so it can be judged that the mouse modeling was successful.

[0101] Table 2-2 Statistics of blood glucose changes in mice during modeling

[0102]

[0103] 2.2.3 Analysis of diabetic mouse modeling results

[0104] Table 2-3 Diabetic mouse modeling results

[0105]

[0106] From the analysis, it can be seen that there is a significant difference in blood sugar between the modeling group mice and the normal group mice, and there is no significant difference in body weight between the groups. The lowest blood sugar of a single mouse in each group is 6.9mmol / L, and the normal blood sugar range is 3.3-6.8mmol / L, which has reached the diabetic blood sugar range. Therefore, it is believed that the blood sugar of a single mouse has reached the diabetic range.

[0107] 2.3 Drug treatment of diabetic mice

[0108] 2.3.1 Body weight changes of diabetic mice after drug administration

[0109] Table 2-4 Statistical table of body weight (g) changes of diabetic mice after drug administration

[0110]

[0111] After successful modeling, mice were divided into groups for treatment, and body weight and blood sugar were measured after the second week after administration. From the third week to the eighth week, the weight of mice in the normal group continued to grow at a low level, and the weight of mice in the model control group and the treatment group increased slowly. In the first week, the weight of the high-dose group, the medium-dose group and the drinking group was high; in the second week, the weight of the drinking group decreased, and the weight of the other groups increased, with the high-dose group having the highest weight; in the third week, the weight of the drinking group increased, and in the fourth week, the weight increase trend of the high-dose group slowed down, the weight of the normal group decreased slightly, and the other groups still showed an increasing trend; in the fifth week, the medium-dose group and the high-dose group had the highest weight; in the sixth week, the high-dose group had the highest weight of 60.42g, and the low-dose group and the normal group had a decrease in weight; in the seventh week, the weight of mice in the high-dose group and the normal group decreased, and the weight of mice in the other groups increased significantly, among which the medium-dose group had the highest weight of 64.97g.

[0112] 2.3.2 Changes in blood glucose in diabetic mice after drug administration

[0113] Table 2-5 Statistics of changes in blood glucose (mmol / L) in diabetic mice after administration

[0114]

[0115] After grouping, the drug treatment was carried out, and the fasting blood sugar was measured every week. The model group served as a control during the treatment period, and the blood sugar showed a steady upward trend. The blood sugar of the model group reached 8.6mmol / L in the seventh week. In the second week after administration, the blood sugar of the mice in the high-dose group showed a slight upward trend, and in the third week, the blood sugar showed a downward trend. In the fourth week, the blood sugar of the mice in the positive drug group, the high-dose group, and the normal group was in the normal range. During the treatment, the blood sugar level of the low-dose group showed a tortuous state, showing a downward trend between the 1st and 3rd weeks and then rising for two consecutive weeks. After the blood sugar level decreased in the sixth week, the blood sugar level increased significantly in the seventh week, reaching 8.5mmol / L, indicating that the treatment effect of the low-dose group was not significant or even no treatment effect. In the fifth week, the blood sugar of the mice in the model group and the low-dose group was still higher than the normal level, and the blood sugar of the other groups was lower than 7mmol / L, which was at a normal level. In the sixth week, the low-dose group returned to the normal level. In the seventh week, except for the high-dose group and the normal group, the blood sugar of the other groups was higher than the normal level, indicating that the treatment effect of the high-dose group was better than that of the positive drug group.

[0116] 2.3.3 Analysis of treatment results in diabetic mice after drug administration

[0117] Table 2-6 Analysis of treatment results of diabetic mice after drug administration

[0118]

[0119] 2.3.4 Analysis of oral glucose tolerance results after 8 weeks of treatment

[0120] Table 2-7 Oral glucose tolerance results after 8 weeks of treatment

[0121]

[0122] On the second day after weaning off food, each group was first tested for fasting blood glucose. At this time, the blood glucose of the low-dose group, the positive drug group and the high-dose group was lower than 6.0mmol / L, the drinking group was 6.4mmol / L, and the model group, the normal group and the medium-dose group were higher than 7.0mmol / L. Blood glucose was measured again 30 minutes after glucose infusion. The blood glucose of each group increased, except for the low-dose group, all were higher than 7.0mmol / L, among which the highest in the normal group was 8.7mmol / L. Blood glucose was measured again at 60 minutes. At this time, the blood glucose of the drinking group did not change, the blood glucose of the positive drug group increased to 8.3mmol / L, and the rest of the groups were in a downward trend. Blood glucose was measured at 120 minutes. The medium-dose group increased slightly to a maximum of 8.1mmol / L, the high-dose group increased significantly, and the rest of the groups still showed a downward trend. From ACU, it can be concluded that the low-dose group has a low glucose tolerance of 11.4, and the medium-dose group has a maximum glucose tolerance of 15.95.

[0123] 3. Analysis of the results of Dongling tea in treating diabetic gene mice

[0124] 3.1db / dbT2DM (diabetes gene defective mice) background value

[0125] The mice used in the experiment were two months old, with a body weight range of approximately 20-35g, and blood sugar values ​​ranging from approximately 4.6-10mmol / L. They were active in action, struggled violently after being caught, were small in size, and had normal food and water intake, making them healthy mice.

[0126] 3.2 Body weight changes in non-diseased db / dbT2DM (type 2 diabetes gene-deficient mice)

[0127] Table 2-8 Record of body weight (g) changes in non-diseased db / dbT2DM (type 2 diabetes gene defective mice)

[0128]

[0129] After one week of adaptive feeding, the body weight of the non-diseased db / dbT2DM mice began to be measured. No grouping was performed from August 27 to October 15. In the first week, the body weight of each group was less than 40g. In the second week, the body weight of the seventh group decreased slightly, and then the body weight of each group increased slowly. The growth trend of the first group was greater than that of the other groups. In the seventh week, the body weight of the first group was higher than 50g, the seventh group was lower than 40g, and the rest of the groups were between 40-50g. It can be concluded that the mice in the first group may be the heaviest of all groups.

[0130] 3.3db / dbT2DM (type 2 diabetes gene defective mice) blood glucose changes

[0131] Table 2-9 Changes in blood glucose (mmol / L) in db / dbT2DM (type 2 diabetes gene defective mice) without disease

[0132]

[0133]

[0134] The fasting blood glucose of db / dbT2DM (type 2 diabetes gene-deficient mice) without disease was recorded. In the first week, the highest blood glucose of the first group was 9.0mmol / L, and the blood glucose of each group was higher than 5mmol / L; in the second week, the blood glucose of all groups except the second group showed an upward trend; in the third week, the blood glucose of the first and seventh groups decreased, while that of the other groups increased, with the highest being 9.6mmol / L in the sixth group; in the fourth week, the blood glucose of all groups increased, with the highest being 10.7mmol / L in the sixth group; in the fifth week, the blood glucose of all groups decreased except the first group, with the highest being 10mmol / L in the first group and the lowest being 6.7mmol / L in the seventh group; in the sixth week, the blood glucose of all groups decreased significantly, with the lowest being 6.8mmol / L and the highest being 9.1mmol / L in the third group; in the seventh week, the blood glucose of all groups increased, with the highest being 12.6mmol / L in the sixth group, and the blood glucose of all groups was higher than 10.0mmol / L, so it was judged that db / dbT2DM (type 2 diabetes gene-deficient mice) had developed the disease.

[0135] 3.4 Body weight changes of db / dbT2DM (type 2 diabetes gene-deficient mice) after treatment

[0136] Table 2-10 Record of changes in body weight (g) of db / dbT2DM (type 2 diabetes gene defective mice) after treatment

[0137]

[0138] After the start of treatment, the mice were divided into groups and given medication, and the body weights of the mice were measured and recorded every week. In the first week, the body weights of all groups were above 40 g, with the lowest being 41.09 g in the low-dose group. In the second and third weeks, the body weights of all groups showed an upward trend, with the highest being 51.73 g in the medium-dose group in the last week. In the fourth week, the body weights of the model group and the low-dose group increased, while those of the other groups decreased. In the fifth week, the body weights of the model group and the positive drug group decreased, while those of the medium-dose group, the low-dose group and the high-dose group increased, with the highest being 51.46 g in the medium-dose group. In the sixth week, all showed an upward trend. In the seventh week, all decreased, with the lowest being 44.56 g in the positive drug group and the highest being 49.94 g in the medium-dose group, with an overall decrease.

[0139] 3.5 Changes in blood glucose in db / dbT2DM (type 2 diabetes gene-deficient mice) after treatment

[0140] Table 2-11 Changes in blood glucose (mmol / L) in db / dbT2DM (type 2 diabetes gene defective mice) after treatment

[0141]

[0142] After group-based medication treatment, fasting blood glucose was measured every week. In the first week, the highest blood glucose in the model group was 12.9mmol / L, and the lowest was 8.6mmol / L in the positive drug group. The blood glucose in each group was higher than 8mmol / L. In the second week, the blood glucose in the high-dose group dropped to a minimum of 8.1mml / L, and the positive drug group slightly dropped to 8.4mmol / L. The medium-dose group dropped significantly, the model group dropped, and the low-dose group increased. In the third week, the blood glucose in the model group dropped significantly, and the growth curve of the low-dose group was gentle, still the highest, with a value of 11.5mmol / L. The other groups all showed an upward trend. In the fourth week, except for the model group, the blood glucose in the model group dropped significantly. All groups showed a downward trend; in the 5th week, the blood sugar level in the positive drug group did not change, the high-dose group decreased slightly, and the other groups showed an upward trend; in the 6th week, all groups showed an upward trend, among which the blood sugar level in the model group was the highest at 16.6mmol / L, and the lowest at 7.4mmol / L in the high-dose group; in the 7th week, all groups showed a downward trend, among which the low-dose group was 7.1mmol / L, and the high-dose group was 10.2mmol / L; From the above analysis, it can be concluded that the high-dose group has the most significant therapeutic effect on db / dbT2DM (type 2 diabetes gene-deficient mice), and the low-dose group has a poor therapeutic effect.

[0143] 3.6 Analysis of treatment results of db / dbT2DM (type 2 diabetes gene-deficient mice) after drug administration

[0144] Table 2-12 Analysis of treatment results of db / dbT2DM (type 2 diabetes gene defective mice) after drug administration

[0145]

[0146]

[0147] After treatment of db / dbT2DM (type 2 diabetes gene defective mice), the mice in the model group had poor appetite, reduced urine output, slow movements, and began to bite mice in the same cage after being deprived of food, and had severe hair loss. Judging from the blood sugar and body weight conditions, the high-dose group and the positive drug group had the most significant treatment effects, and the high-dose group had a better treatment effect than the positive drug group.

[0148] 3.7 Analysis of Glycated Blood Glucose Protein and Insulin Results after 14 Weeks of Treatment

[0149] Table 2-13 Analysis of Glycated Blood Glucose Protein and Insulin Results after 14 Weeks of Treatment

[0150]

[0151] From the above results, insulin levels were all low. Except for the low-dose group, the detection levels of the other groups were all less than 0.05. Glycated blood glucose protein was higher in the medium-dose group, while the other groups were within the normal range. The low-dose group and the positive drug group were slightly better than the high-dose group and the model group.

[0152] 4. Conclusion

[0153] (I) Evaluation of the therapeutic effect of Dongling tea on diabetic mice with high fat and high sugar content

[0154] 1. After 7 weeks of high-fat and high-sugar modeling, the blood sugar level of the modeled mice was between 7.6 and 7.8, while the average blood sugar level of mice on a normal diet was 6.8, indicating that a high-fat and high-sugar diet-induced diabetes model in mice has been formed.

[0155] 2. After 8 weeks of treatment, the blood sugar levels of mice treated with high-fat and high-sugar drugs returned to normal levels in both the positive drug group and the mice treated with Dongling tea. The performance of the free drinking group was basically the same as that of the positive drug group. Therefore, Dongling tea, as a tea drink, has a good advantage in controlling blood sugar if taken regularly.

[0156] 3. Dongling tea and positive drugs have little effect on the body weight and morphological indicators (such as morphology and drinking behavior) of experimental mice.

[0157] (II) Evaluation of the therapeutic effect of Dongling tea on type 2 diabetes absent gene mice

[0158] 1. After the third week of feeding, the blood sugar of type 2 diabetic mice began to rise, and then the blood sugar of mice gradually increased, but the blood sugar of some mice still tended to normal levels. Until the seventh week, all mice became ill and began to receive drug treatment. In this group of experiments, the tea powder of Diboer Tea Zhen produced by Tasly with the function of lowering blood sugar was used as the positive drug control group.

[0159] 2. From the average level of each group, the high-dose group of Dongling Tea had a better therapeutic effect and was better than the positive drug control group. Among all the observation groups, only the high-dose Dongling Tea had a normal average blood sugar level of 6.9 and 6.56 in the 4th and 5th weeks after treatment. Although the blood sugar of the other observation groups, such as the positive drug control group and the medium-dose group, decreased during the treatment, it did not return to normal levels.

[0160] 3. Whether it is a positive drug or Dongling tea, there are individual differences in the effects of drugs on mice, such as

[0161] The average blood sugar level of mice in the high-dose group was 7.1 after 7 weeks of treatment, slightly higher than the normal value. However, at the individual level, there were 8 mice in this group, of which 5 mice had normal blood sugar levels, which were 5.6, 4.9, 4.3, 6.8, and 4.5, respectively, and the other 3 mice had blood sugar levels of 10.0, 11.1, and 9.7. This phenomenon also existed in other observation groups.

[0162] The embodiments of the present application have been described above, and the above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and changes will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The selection of terms used herein is intended to best explain the principles of the embodiments, practical applications, or improvements to the technology in the market, or to enable other persons of ordinary skill in the art to understand the embodiments disclosed herein.

Claims

1. A composition having a blood sugar lowering effect, characterized in that: The following ingredients are prepared by weight: 1 part of Ophiopogon japonicus, 1 part of Corn Silk, 2 parts of Yunfuling, 2 parts of Pu'er Tea Old Yellow Tablets, 2 parts of Pu'er Raw Tea, and 2 parts of Mulberry Leaves; The preparation method of the composition having the hypoglycemic effect comprises the following steps: (1) Weighing: Weigh ophiopogon japonicus, corn silk, Yunfuling, Pu'er tea old yellow slices, Pu'er raw tea, and mulberry leaves according to the weight of each raw material; (2) Add 8 times the weight of water to Radix Ophiopogonis, Corn Silk, and Poria Cocos, and boil for 1.5-2 hours, then add Pu'er Tea Old Yellow Tablets, Pu'er Raw Tea, and Mulberry Leaves, and boil for another 0.5-1 hour; (3) The filtrate is passed through a 200-mesh sieve and concentrated to a paste, and then dried to obtain an extract; (4) The obtained extract is crushed and packaged to obtain a composition having a blood sugar lowering effect.

2. A composition having a hypoglycemic effect according to claim 1, characterized in that: The specific operation of concentrating the filtrate through a 200-mesh sieve to a paste is to pass the decocted filtrate through a 200-mesh sieve, and then concentrate under reduced pressure. When the filtrate is concentrated under reduced pressure to 50° C., the relative density is measured to be 1.20-1.

25.

3. A composition having a hypoglycemic effect according to claim 2, characterized in that: The concentration temperature of the reduced pressure concentration is 70° C. and the vacuum degree is -0.06 MPa.

4. A composition having a hypoglycemic effect according to claim 1, characterized in that: The drying temperature in step (3) is 70-80°C.

5. A composition having a hypoglycemic effect according to claim 1, characterized in that: In the step (4), the extract obtained is crushed and the powder is packaged, with 0.5 g per bag for inner packaging and 10 bags per bag for outer packaging.

Images