Microbial water substrate modifier and application thereof

Abstract

The invention relates to a microbial water body substrate modifier and application thereof. The microbial water body substrate modifier comprises the following components: compound microbial powder, zeolite powder, shell powder and polyferric sulfate, the compound microbial powder comprises bacillus powder and lactic acid bacteria powder. The microbial water substrate modifier can effectively realize rapid bottom modification of aquaculture water, improve water quality, optimize aquaculture environment, reduce diseases and promote healthy growth of aquaculture animals. Wherein the bacillus can flocculate and settle residual organic matters such as feed, animal waste and the like in the culture water body by utilizing the flocculation effect of the bacillus, and the physical adsorption components, namely the zeolite powder and the shell powder, and the chemical adsorption component, namely the polyferric sulfate are synergistically matched, so that suspended organic matter large particles in the water body are effectively adsorbed and settled; and the organic matters are jointly degraded through the common graded degradation effect of the bacillus and the lactic acid bacteria, so that the effects of improving the substrate of the aquaculture water body and promoting the healthy growth of the aquaculture animals are achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture and relates to a microbial water body bottom quality improver and application thereof. Background Art

[0002] Ponds are not only the growth environment for farmed fish and other aquatic organisms, but also the treatment sites for their secretions and excrement. As the density of aquaculture continues to increase, the farming model that consumes a large amount of high-protein feed to maintain production has destroyed the original ecological balance of the pond. At the same time, the amount of leftover bait, feces, corpses, and dead algae of aquatic organisms formed after a large amount of feeding continues to increase and deposit at the bottom of the pond, forming a layer of black sludge at the bottom of the pond. The organic matter in the sludge ferments and decomposes under anaerobic conditions to produce ammonia, hydrogen sulfide, mercaptans and other substances. The nitrite in the water body seriously exceeds the standard, directly or indirectly endangering farmed fish and aquatic organisms, and even causing poisoning and death of farmed animals, seriously affecting the output of farmed products, reducing farmed income or causing losses.

[0003] Commonly used methods for improving water bottom quality are physical, chemical and biological. Compared with biological methods, physical and chemical methods are faster, more direct and more effective, but they also have some disadvantages. For example, physical methods cause greater disturbance to the pond, require more work, and are prone to waste of resources. Chemical methods are prone to secondary pollution such as chemical residues, causing ecological damage, damage to farmed animals, and affecting farming results. Compared with them, biological methods have shown unique advantages, such as safety and environmental protection, good maintenance of ecological balance, and good biosafety. Biological bottom improvement refers to the use of microbial preparations and other methods to improve the bottom environment of the pond, decompose the bottom organic matter, and reduce the accumulation of pollutants by using the life activities of beneficial microorganisms. However, most of the current biological bottom improvement technologies and products also have some problems, such as slow effect, poor stability, and strong dependence on the environment, which make it difficult to promote them on a large scale. Therefore, it is very necessary to develop more biological bottom improvement strategies that are quick to take effect. Summary of the invention

[0004] In view of the deficiencies in the prior art, the object of the present invention is to provide a microbial water body bottom quality improver and its application, and in particular to a microbial water body bottom quality improver and its application in improving aquaculture water quality and / or improving aquaculture production performance.

[0005] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a microbial water body bottom quality improver, the components of which include composite microbial powder, zeolite powder, shell powder, and polymerized ferric sulfate; the composite microbial powder includes Bacillus powder and lactic acid bacteria powder.

[0007] The microbial water body bottom quality improver involved in the present invention creatively combines bacillus powder, lactic acid bacteria powder with zeolite powder, shell powder and polyferric sulfate, which can effectively realize the rapid bottom improvement of aquaculture water, improve water quality, optimize the breeding environment, reduce diseases, and promote the healthy growth of aquaculture animals. Among them, bacillus can flocculate and settle organic matter such as feed and animal feces remaining in the breeding water by its own flocculation effect, and the physical adsorption components zeolite powder and shell powder cooperate with the chemical adsorption component polyferric sulfate to effectively adsorb and settle large particles of suspended organic matter in the water body, and then the organic matter is degraded together by the common graded degradation of bacillus and lactic acid bacteria, thereby achieving the effect of improving the bottom quality of the breeding water body and promoting the healthy growth of aquaculture animals. The production process of this product is simple, and only the above-mentioned raw materials need to be mixed evenly. The production cost and energy consumption are low, and it is easy to mass produce. The final effective live bacteria count is high, the shelf life is long, and the adaptability is strong. It solves the problems of unstable product quality, unsatisfactory use effect, low survival rate of effective live bacteria count and high production cost that exist in the current bottom soil improvers for aquaculture on the market.

[0008] Preferably, the components of the microbial water body substrate improver include, by mass, 10-20 parts of composite microbial powder, 30-40 parts of zeolite powder, 20-30 parts of shell powder, and 20-30 parts of polyferric sulfate.

[0009] The mass fraction of the composite microbial powder can be selected from 10 parts, 12 parts, 13 parts, 15 parts, 16 parts, 18 parts, 20 parts, etc.; the mass fraction of the zeolite powder can be selected from 30 parts, 32 parts, 33 parts, 35 parts, 36 parts, 38 parts, 40 parts, etc.; the mass fraction of the shell powder can be selected from 20 parts, 22 parts, 23 parts, 25 parts, 26 parts, 28 parts, 30 parts, etc.; the mass fraction of the polymerized ferric sulfate can be selected from 20 parts, 22 parts, 23 parts, 25 parts, 26 parts, 28 parts, 30 parts, etc.; other specific point values ​​within the above numerical ranges can be selected, and they will not be repeated here one by one.

[0010] Preferably, the Bacillus powder is selected from any one of Bacillus coagulans powder, Bacillus subtilis powder and Paenibacillus polymyxa powder, or a combination of at least two thereof, more preferably a combination of Bacillus coagulans powder, Bacillus subtilis powder and Paenibacillus polymyxa powder.

[0011] When the Bacillus powder in the microbial water body bottom quality improver of the present invention is a combination of Bacillus coagulans powder, Bacillus subtilis powder and Bacillus polymyxa powder, it has a better effect in flocculating and settling organic matter and degrading organic matter together with lactic acid bacteria, thereby improving the bottom quality of aquaculture water bodies. Furthermore, when the use ratio of the three strains is in the following range, the effect is more significant.

[0012] Preferably, the ratio of the number of live bacteria of Bacillus coagulans, Bacillus subtilis and Paenibacillus polymyxa in the Bacillus powder is (2-10):(1-2):(1-2).

[0013] The specific point values ​​in (2-10) can be selected from 2, 3, 4, 5, 6, 7, 8, 9, 10, etc.; the specific point values ​​in (1-2) can be selected from 1, 1.2, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, etc.; other specific point values ​​within the above numerical ranges can be selected and will not be listed here one by one.

[0014] Preferably, the lactic acid bacteria powder is selected from any one of Lactobacillus plantarum powder, Pediococcus acidilactici powder or Lactobacillus acidophilus powder, or a combination of at least two thereof, and more preferably a combination of Lactobacillus plantarum powder, Pediococcus acidilactici powder and Lactobacillus acidophilus powder.

[0015] When the lactic acid bacteria powder in the microbial water body substrate improver of the present invention is a combination of Lactobacillus plantarum powder, Pediococcus acidilactici powder and Lactobacillus acidophilus powder, it has a better effect in cooperating with Bacillus to degrade organic matter, thereby improving the bottom quality of aquaculture water bodies. Further, when the use ratio of the three strains is in the following range, the effect is more significant.

[0016] Preferably, the ratio of the number of viable bacteria of Lactobacillus plantarum, Pediococcus acidilactici and Lactobacillus acidophilus in the lactic acid bacteria powder is (5-10):(1-5):1.

[0017] The specific point values ​​in (5-10) can be selected from 5, 6, 7, 8, 9, 10, etc.; the specific point values ​​in (1-5) can be selected from 1, 2, 3, 4, 5, etc.; other specific point values ​​within the above numerical ranges can be selected, and they will not be listed here one by one.

[0018] Preferably, the number of viable bacteria in the Bacillus coagulans powder, Bacillus subtilis powder, and Bacillus polymyxa powder is not less than 10 8 CFU / g, for example 1×10 8 CFU / g, 3×10 8 CFU / g, 5×10 8 CFU / g, 1×10 9 CFU / g, 5×10 9 CFU / g, 1×10 10 CFU / g, 1×10 11 CFU / g, etc. Other specific point values ​​within this numerical range can be selected and will not be described here one by one.

[0019] Preferably, the number of viable bacteria in the Lactobacillus plantarum powder, Pediococcus acidilactici powder, and Lactobacillus acidophilus powder is not less than 10 8 CFU / g, for example 1×10 8 CFU / g, 3×10 8 CFU / g, 5×10 8 CFU / g, 1×10 9 CFU / g, 5×10 9 CFU / g, 1×10 10 CFU / g, 1×10 11 CFU / g, etc. Other specific point values ​​within this numerical range can be selected and will not be described here one by one.

[0020] Preferably, the ratio of the number of live bacteria of Bacillus coagulans, Bacillus subtilis, Paenibacillus polymyxa, Lactobacillus plantarum, Pediococcus acidilactici to Lactobacillus acidophilus in the composite microbial powder is (10-50):(5-10):(5-10):(5-10):(1-5):1.

[0021] The specific point values ​​in (10-50) can be selected from 10, 15, 20, 25, 30, 35, 40, 45, 50, etc.; the specific point values ​​in (5-10) can be selected from 5, 6, 7, 8, 9, 10, etc.; the specific point values ​​in (1-5) can be selected from 1, 2, 3, 4, 5, etc.; other specific point values ​​within the above numerical ranges can be selected, and they will not be listed here one by one.

[0022] Preferably, the composite microbial powder is prepared by a method comprising the following steps:

[0023] The required strains are inoculated into culture medium respectively for activation and fermentation culture in sequence to obtain fermentation liquid; the fermentation liquid is centrifuged respectively, mixed with protective agent and then dried to obtain different bacterial powders; the different bacterial powders are mixed according to the ratio of live bacterial count to obtain the composite microbial powder.

[0024] Preferably, the protective agent is selected from any one or a combination of at least two of skim milk, gelatin, dextrin, gum arabic, dextran, sodium alginate, polyvinyl pyrrolidone, sucrose, lactose, trehalose, sorbitol or xylitol.

[0025] Preferably, the components of the microbial water body substrate improver also include any one or a combination of at least two of sodium humate, chitosan, potassium humate, bentonite, calcium peroxide, diatomaceous earth or medical stone.

[0026] In a second aspect, the present invention provides use of the microbial water body substrate improver according to the first aspect in improving aquaculture water quality and / or enhancing aquaculture production performance.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The microbial water body bottom quality improver involved in the present invention creatively combines bacillus powder, lactic acid bacteria powder with zeolite powder, shell powder and polyferric sulfate, which can effectively realize the rapid bottom improvement of aquaculture water, improve water quality, optimize the breeding environment, reduce diseases, and promote the healthy growth of aquaculture animals. Among them, bacillus can flocculate and settle organic matter such as feed and animal feces remaining in the breeding water by its own flocculation effect, and the physical adsorption components zeolite powder and shell powder cooperate with the chemical adsorption component polyferric sulfate to effectively adsorb and settle large particles of suspended organic matter in the water body, and then the organic matter is degraded together by the common graded degradation of bacillus and lactic acid bacteria, thereby achieving the effect of improving the bottom quality of the breeding water body and promoting the healthy growth of aquaculture animals. The production process of this product is simple, and only the above-mentioned raw materials need to be mixed evenly. The production cost and energy consumption are low, and it is easy to mass produce. The final effective live bacteria count is high, the shelf life is long, and the adaptability is strong. It solves the problems of unstable product quality, unsatisfactory use effect, low survival rate of effective live bacteria count and high production cost that exist in the current bottom soil improvers for aquaculture on the market. DETAILED DESCRIPTION

[0029] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0030] The zeolite powder used in the following Examples 1-3 is a zeolite powder product with a model of 100 mesh purchased from Xinyang Shanhe New Materials Co., Ltd.;

[0031] The shell powder used in the following Examples 1-3 is a 100-mesh shell powder product purchased from Shuntian Mineral Products Processing Plant in Lingshou County;

[0032] The polyferric sulfate used in the following Examples 1-3 is a 100-mesh polyferric sulfate product purchased from Henan Sairuike Biotechnology Co., Ltd.;

[0033] The Bacillus coagulans used in the following Example 1 is the BC66 strain, which is classified and named Bacillus coagulans. The depository unit is the General Microbiology Center of the China Microbiological Culture Collection Administration, the deposit time is March 8, 2021, the deposit number is CGMCC No. 21879, and the address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the Bacillus coagulans used in the following Example 2 is the ATCC7050 strain; the Bacillus coagulans used in the following Example 3 is the BC66 strain.

[0034] The Bacillus subtilis used in the following Example 1 is the ATCC10719 strain; the Bacillus subtilis used in the following Example 2 is the ATCC21394 strain; and the Bacillus subtilis used in the following Example 3 is the ATCC6633 strain.

[0035] The Paenibacillus polymyxa used in the following Example 1 is the ATCC43865 strain; the Paenibacillus polymyxa used in the following Example 2 is the ATCC15970 strain; and the Paenibacillus polymyxa used in the following Example 3 is the ATCC43865 strain.

[0036] The plant lactobacillus used in the following embodiment 1 is the Lp90 strain, which is classified and named as plant lactobacillus Lactobacillus plantarum, and the deposit unit is the General Microbiological Center of the China Microorganism Culture Collection Administration Committee, the deposit time is January 27, 2015, the deposit number is CGMCC No.10453, and the address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; the plant lactobacillus used in the following embodiment 2 is the ATCC8014 strain; and the plant lactobacillus used in the following embodiment 3 is the ATCC14917 strain.

[0037] The Pediococcus acidilactici used in the following Example 1 is the PA153 strain, which is classified and named Pediococcus acidilactici; the preservation unit is the China Center for Type Culture Collection, the preservation time is March 02, 2023, the preservation number is CCTCC No.M2023230, and the address is: Wuhan University, Wuhan, China; the Pediococcus acidilactici used in the following Example 2 is the ATCC8042 strain; the Pediococcus acidilactici used in the following Example 3 is the PA153 strain.

[0038] The Lactobacillus acidophilus used in the following Example 1 is the LA71 strain, which is classified and named as Lactobacillus acidophilus Lactobacillus acidophilus, and the preservation unit is the General Microbiology Center of the China Microorganism Culture Collection Administration Committee. The preservation time is January 29, 2021, the preservation number is CGMCC No.21765, and the address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the Lactobacillus acidophilus used in the following Example 2 is the ATCC4356 strain; the Lactobacillus acidophilus used in the following Example 3 is the ATCC4357 strain.

[0039] The culture medium formula involved in the following content is as follows:

[0040] MRS medium (g / L): peptone 10g / L, beef extract powder 8g / L, glucose 20g / L, yeast extract powder 4g / L, diammonium hydrogen citrate 2g / L, K2HPO4 2g / L, MgSO4 0.2g / L, MnSO4 0.04g / L, Tween 80 1mL / L, sodium acetate 5g / L.

[0041] The following content involves the method for preparing the bacterial powder as follows:

[0042] (1) Bacillus coagulans, Bacillus subtilis, Paenibacillus polymyxa, Lactobacillus plantarum, Pediococcus acidilactici and Lactobacillus acidophilus stored in -80°C glycerol tubes were inoculated at 5% into 5 mL of sterile MRS medium, cultured at 37°C for 24 h, and subcultured twice to obtain activated strains; then, the activated strains were inoculated at 5% into 50 mL of sterile MRS medium, cultured at 37°C for 12 h, and expanded culture fluid was obtained.

[0043] (2) The culture medium was expanded and inoculated into 1000 mL of sterile MRS medium at a 3% inoculum and cultured at 37° C. During the culture process, the bacterial concentration of the fermentation liquid was monitored and the culture time of each strain was adjusted to ensure that the bacterial concentration was roughly consistent.

[0044] (3) The obtained fermentation broth was centrifuged at 5000 r / min for 20 min at 4°C, the supernatant was discarded under sterile conditions, and the bacterial sludge was collected; the collected bacterial sludge was mixed with a protective agent (8% xylo-oligosaccharides, 15% skim milk powder, and the remainder was water) in a volume ratio of 1:2 using a vortex to obtain a mixture of each strain and the protective agent.

[0045] (4) The mixtures were pre-frozen in a -80°C refrigerator for 3 h to obtain pre-frozen live bacterial preparations; then, the pre-frozen live bacterial preparations were freeze-dried in a vacuum freeze dryer at a freeze-drying temperature of -50°C and a vacuum degree of 0.20 mBar for 48 h until completely dry, to obtain Bacillus coagulans powder, Bacillus subtilis powder, Bacillus polymyxa powder, Lactobacillus plantarum powder, Pediococcus acidilactici powder, and Lactobacillus acidophilus powder, with the number of live bacteria being 5×10 9 CFU / g. Store in a -4℃ refrigerator for later use.

[0046] Example 1

[0047] The present embodiment provides a microbial water body substrate improver, the components of which include 15 parts of composite microbial powder, 35 parts of zeolite powder, 25 parts of shell powder, and 25 parts of polyferric sulfate by mass; the composite microbial powder is composed of Bacillus coagulans powder, Bacillus subtilis powder, Paenibacillus polymyxa powder, Lactobacillus plantarum powder, Pediococcus acidilactici powder, and Lactobacillus acidophilus powder in a ratio of 30:10:10:5:5:1 of viable bacteria. The above-prepared raw materials are put into a mixer and mixed evenly.

[0048] Example 2

[0049] The present embodiment provides a microbial water body substrate improver, the components of which include 20 parts of composite microbial powder, 40 parts of zeolite powder, 30 parts of shell powder, and 30 parts of polyferric sulfate by mass; the composite microbial powder is composed of Bacillus coagulans powder, Bacillus subtilis powder, Paenibacillus polymyxa powder, Lactobacillus plantarum powder, Pediococcus acidilactici powder, and Lactobacillus acidophilus powder in a ratio of 20:5:5:10:1:1 of viable bacteria. The above-prepared raw materials are put into a mixer and mixed evenly.

[0050] Example 3

[0051] The present embodiment provides a microbial water body substrate improver, the components of which include 10 parts of composite microbial powder, 30 parts of zeolite powder, 20 parts of shell powder, and 20 parts of polyferric sulfate by weight; the composite microbial powder is composed of Bacillus coagulans powder, Bacillus subtilis powder, Paenibacillus polymyxa powder, Lactobacillus plantarum powder, Pediococcus acidilactici powder, and Lactobacillus acidophilus powder in a ratio of 40:10:10:10:5:1 of viable bacteria. The above-prepared raw materials are put into a mixer and mixed evenly.

[0052] Example 4

[0053] This embodiment provides a microbial water body bottom quality improver, the components of which differ from those of embodiment 1 only in that Bacillus coagulans powder is missing, and the reduced mass is proportionally distributed to the mass of Bacillus subtilis powder and Paenibacillus polymyxa powder, while the other components and contents remain unchanged. The above-prepared raw materials are put into a mixer and mixed evenly to obtain the product.

[0054] Example 5

[0055] This embodiment provides a microbial water body bottom quality improver, the components of which differ from those of embodiment 1 only in that Bacillus subtilis powder is missing, and the reduced mass is proportionally distributed to the mass of Bacillus coagulans powder and Paenibacillus polymyxa powder, while the other components and contents remain unchanged. The above-prepared raw materials are put into a mixer and mixed evenly to obtain the product.

[0056] Example 6

[0057] This embodiment provides a microbial water body bottom quality improver, the components of which differ from those of embodiment 1 only in that the polymyxa bacillus powder is missing, and the reduced mass is proportionally distributed to the mass of the coagulant bacillus powder and the subtilis bacillus powder, while the other components and contents remain unchanged. The above-prepared raw materials are put into a mixer and mixed evenly to obtain the product.

[0058] Example 7

[0059] This embodiment provides a microbial water body substrate improver, the components of which differ from those of embodiment 1 only in that Lactobacillus plantarum powder is missing, and the reduced mass is proportionally distributed to the mass of Pediococcus acidilactici powder and Lactobacillus acidophilus powder, while the other components and contents remain unchanged. The above-prepared raw materials are put into a mixer and mixed evenly to obtain the product.

[0060] Example 8

[0061] This embodiment provides a microbial water body substrate improver, the components of which differ from those of embodiment 1 only in that the lactic acid Pediococcus powder is missing, and the reduced mass is proportionally distributed to the mass of plant lactobacillus powder and acidophilus powder, while the other components and contents remain unchanged. The above-prepared raw materials are put into a mixer and mixed evenly to obtain the product.

[0062] Example 9

[0063] This embodiment provides a microbial water body substrate improver, the components of which differ from those of embodiment 1 only in that Lactobacillus acidophilus powder is missing, and the reduced mass is proportionally distributed to the mass of Lactobacillus plantarum powder and Pediococcus acidilactici powder, while the other components and contents remain unchanged. The above-prepared raw materials are put into a mixer and mixed evenly to obtain the product.

[0064] Comparative Example 1

[0065] This comparative example provides a microbial water body substrate improver, the components of which differ from those of Example 1 only in that the three types of Bacillus powder are missing, and the reduced mass is proportionally distributed to the mass of the three types of lactic acid bacteria powder, while the other components and contents remain unchanged. The above-prepared raw materials are put into a mixer and mixed evenly to obtain the product.

[0066] Comparative Example 2

[0067] This comparative example provides a microbial water body substrate improver, the components of which differ from those of Example 1 only in that the three lactic acid bacteria powders are missing, and the reduced mass is proportionally distributed to the mass of the three Bacillus powders, while the other components and contents remain unchanged. The above-prepared raw materials are put into a mixer and mixed evenly to obtain the product.

[0068] Comparative Example 3

[0069] This comparative example provides a microbial water body substrate improver, the components of which differ from those of Example 1 only in that zeolite powder is missing, and the reduced mass thereof is proportionally distributed to the mass of shell powder and polyferric sulfate, while the other components and contents remain unchanged. The above-prepared raw materials are put into a mixer and mixed evenly to obtain the product.

[0070] Comparative Example 4

[0071] This comparative example provides a microbial water body substrate improver, the components of which differ from those of Example 1 only in that shell powder is missing, and the reduced mass thereof is proportionally distributed to the mass of zeolite powder and polyferric sulfate, while the other components and contents remain unchanged. The above-prepared raw materials are put into a mixer and mixed evenly to obtain the product.

[0072] Comparative Example 5

[0073] This comparative example provides a microbial water body substrate improver, the components of which differ from those of Example 1 only in the lack of polyferric sulfate, the reduced mass of which is proportionally distributed to the mass of zeolite powder and shell powder, and the other components and contents remain unchanged. The above-prepared raw materials are put into a mixer and mixed evenly to obtain a product.

[0074] Test Example 1

[0075] 15 groups of hairy crab breeding ponds with consistent breeding conditions were selected as test ponds. The microbial water body bottom quality improvers prepared in Examples 1-9 and Comparative Examples 1-5 were used in groups 1-14, respectively, at an amount of 300 g / mu. The control group 15 groups did not use the product. The ammonia nitrogen content and nitrite content in the pond water were counted before using the product and 3 days and 7 days after using the product. The ammonia nitrogen degradation rate (%) and nitrite degradation rate (%) after 3 days and 7 days were calculated. The test results are shown in Tables 1 and 2.

[0076] Table 1

[0077]

[0078]

[0079] It can be seen from the data results in Table 1 that the microbial water body bottom quality improver involved in the present invention has an excellent effect of promoting the degradation of ammonia nitrogen in aquaculture pond water, and the formula selection of microorganisms in the improver has an important influence on the above effect.

[0080] Table 2

[0081]

[0082]

[0083] It can be seen from the data results in Table 2 that the microbial water body bottom quality improver involved in the present invention has an excellent effect of promoting the degradation of nitrite in aquaculture pond water, and the formula selection of microorganisms in the improver has an important influence on the above effect.

[0084] Test Example 2

[0085] The bottom area is 0.2m 2 The experiment of outdoor breeding of Penaeus vannamei was carried out in a round barrel with a volume of 2000L. The initial body length of the juvenile shrimp was 1.8±0.1cm, the breeding density was 500 per barrel, and the bottom of the barrel was laid with a thickness of 7cm as the bottom. The barrel for breeding was installed with a shade cloth and an air stone for inflation. Seawater was used as the water source, and no water was exchanged with the outside world except for replenishing the evaporated water (tap water) during the entire breeding period. Commercial Penaeus vannamei feed was used for feeding twice a day, and the feeding rate was 6% of the shrimp body weight.

[0086] The experiment was divided into 15 groups, and the experimental breeding period was 90 days. On the 7th day, groups 1-14 used the microbial water body bottom quality improvers prepared in Examples 1-9 and Comparative Examples 1-5 to treat the breeding system for the first time, with the usage amount of 2g / m 2 Then, an equal amount of microbial water body bottom conditioner was added to the aquaculture system every 7 days until the end of the experiment. No conditioner was added to the control group.

[0087] At the end of the experiment, the growth parameters of each group of whiteleg shrimp were measured, including shrimp production, survival rate and feed conversion rate. The results are shown in Table 3.

[0088] Table 3

[0089] Group Survival rate (%) Feed coefficient Example 1 84.8 1.45 Example 2 83.6 1.39 Example 3 83.2 1.42 Example 4 70.8 1.58 Example 5 76.2 1.63 Example 6 77.6 1.55 Example 7 79.6 1.66 Example 8 80.2 1.57 Example 9 77.4 1.60 Comparative Example 1 62.4 1.84 Comparative Example 2 66.2 1.77 Comparative Example 3 73.8 1.69 Comparative Example 4 77.0 1.71 Comparative Example 5 75.8 1.62 Control group 56.8 2.06

[0090] It can be seen from the data results in Table 3 that the microbial water body bottom quality improver involved in the present invention has an excellent effect of improving the production performance of aquatic animals, and the formula selection of microorganisms in the improver has an important influence on the above effect.

[0091] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0092] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0093] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A microbial water body substrate improver, characterized in that: The components of the microbial water body bottom quality improver include composite microbial powder, zeolite powder, shell powder and polyferric sulfate; the composite microbial powder includes bacillus powder and lactic acid bacteria powder.

2. The microbial water body substrate improver according to claim 1, characterized in that: The components of the microbial water body bottom quality improver include, by weight, 10-20 parts of composite microbial powder, 30-40 parts of zeolite powder, 20-30 parts of shell powder, and 20-30 parts of polyferric sulfate.

3. The microbial water body substrate improver according to claim 1 or 2, characterized in that: The Bacillus powder is selected from any one of Bacillus coagulans powder, Bacillus subtilis powder and Paenibacillus polymyxa powder, or a combination of at least two thereof; more preferably, a combination of Bacillus coagulans powder, Bacillus subtilis powder and Paenibacillus polymyxa powder; Preferably, the ratio of the number of live bacteria of Bacillus coagulans, Bacillus subtilis and Paenibacillus polymyxa in the Bacillus powder is (2-10):(1-2):(1-2).

4. The microbial water body bottom quality improver according to any one of claims 1 to 3, characterized in that: The lactic acid bacteria powder is selected from any one of Lactobacillus plantarum powder, Pediococcus acidilactici powder or Lactobacillus acidophilus powder, or a combination of at least two thereof; more preferably, a combination of Lactobacillus plantarum powder, Pediococcus acidilactici powder and Lactobacillus acidophilus powder; Preferably, the ratio of the number of live bacteria of Lactobacillus plantarum, Pediococcus acidilactici and Lactobacillus acidophilus in the lactic acid bacteria powder is (5-10):(1-5):

1.

5. The microbial water body bottom quality improver according to claim 3, characterized in that: The number of live bacteria in the Bacillus coagulans powder, Bacillus subtilis powder and Paenibacillus polymyxa powder is not less than 10 8 CFU / g.

6. The microbial water body substrate improver according to claim 4, characterized in that: The number of live bacteria in the plant lactobacillus powder, the lactic acid Pediococcus powder and the acidophilus Lactobacillus powder is not less than 10 8 CFU / g.

7. The microbial water body substrate improver according to any one of claims 1 to 6, characterized in that: The ratio of the number of live bacteria of Bacillus coagulans, Bacillus subtilis, Paenibacillus polymyxa, Lactobacillus plantarum, Pediococcus acidilactici to Lactobacillus acidophilus in the composite microbial powder is (10-50):(5-10):(5-10):(5-10):(1-5):

1.

8. The microbial water body substrate improver according to any one of claims 1 to 7, characterized in that: The composite microbial powder is prepared by a method comprising the following steps: The desired strains are inoculated into culture medium respectively for activation and fermentation culture in sequence to obtain fermentation broth; the fermentation broth is centrifuged respectively, mixed with a protective agent and then dried to obtain different bacterial powders; the different bacterial powders are mixed according to the ratio of the number of live bacteria to obtain the composite microbial powder; Preferably, the protective agent is selected from any one or a combination of at least two of skim milk, gelatin, dextrin, gum arabic, dextran, sodium alginate, polyvinyl pyrrolidone, sucrose, lactose, trehalose, sorbitol or xylitol.

9. The microbial water body substrate improver according to any one of claims 1 to 8, characterized in that: The components of the microbial water body substrate improver also include any one of sodium humate, chitosan, potassium humate, bentonite, calcium peroxide, diatomaceous earth or medical stone, or a combination of at least two of them.

10. Use of the microbial water body bottom quality improver according to any one of claims 1 to 9 in improving aquaculture water quality and / or improving aquaculture production performance.

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