Application of glutamine in treatment of soybean meal enteritis of micropterus salmoides

Abstract

The invention discloses innovative application of glutamine in treatment of soybean meal enteritis of micropterus salmoides, and relates to the technical field of feed additives. The core of the technical scheme is to develop a feed additive, and the feed additive comprises glutamine and aims to improve the nutritional value of animal feed and the overall health level of animals. The alanyl-glutamine is added into the soybean meal type feed, so that the growth performance of the largemouth bass is remarkably improved, the health states of intestinal structures and organelles are improved, and the symptoms of soybean meal type enteritis are effectively relieved; the breakthrough achievement brings remarkable economic benefits and ecological values to the feed industry and the aquaculture industry.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, and more particularly to application of glutamine in treating soybean meal-type enteritis in largemouth seabass. Background Art

[0002] Glutamine (Gln), as a conditionally essential amino acid, plays an important role in animal nutrition and health. It is not only the main energy source for intestinal epithelial cells and immune cells, but also participates in the regulation of the mucosal immune system. In mammals, glutamine has been widely used in the treatment of inflammatory bowel disease, showing its importance in maintaining intestinal health.

[0003] Largemouth bass (Micropterus salmoides) is an important carnivorous economic fish. The use of soybean meal-based feed in its aquaculture process often leads to enteritis problems, which not only affects the growth performance of fish, but also may lead to a high mortality rate. The occurrence of soybean meal-based enteritis is related to the anti-nutritional factors in soybean meal, which can cause intestinal inflammation and structural damage, thereby affecting the health and growth of largemouth bass, seriously affecting the economic benefits and product quality of the aquaculture industry.

[0004] Therefore, the present invention aims to provide an application of glutamine in treating soybean meal-type enteritis in largemouth sea bass to solve the above problems. Summary of the invention

[0005] The purpose of the present invention is to provide an application of glutamine in treating soybean meal-type enteritis of largemouth seabass. The present invention not only significantly improves the growth performance of largemouth seabass, but also improves the health status of intestinal structure and organelles, and effectively alleviates the symptoms of soybean meal-type enteritis by adding alanyl-glutamine to soybean meal-type feed.

[0006] The above technical purpose of the present invention is achieved through the following technical scheme: application of glutamine in treating soybean meal enteritis of largemouth bass, a feed additive, the feed additive comprising glutamine, for improving the nutritional value of animal feed and the health status of animals.

[0007] The invention also provides an application of glutamine in preparing an animal feed additive.

[0008] In summary, the present invention has the following beneficial effects:

[0009] 1. The present invention can significantly increase the weight gain rate of largemouth bass and reduce the feed coefficient by adding alanyl-glutamine to soybean meal feed, while alleviating intestinal inflammation caused by soybean meal feed and improving the growth performance of largemouth bass;

[0010] 2. The present invention adds alanyl-glutamine to soybean meal feed, and through experimental verification, it is shown that the addition of alanyl-glutamine can significantly increase the width of intestinal villi and improve the intestinal tissue structure, while alleviating the pathological phenomena such as intestinal villus shedding, nuclear pyknosis, goblet cell vacuolation, mitochondrial matrix dissolution and double membrane dissolution induced by soybean meal feed, thereby proving that glutamine can improve the intestinal health of largemouth bass;

[0011] 3. A feed additive involved in the present invention is unique in that its main ingredient is glutamine, which is intended to enhance the nutritional value of animal feed and further optimize the health status of animals;

[0012] 4. The present invention explores the innovative application of glutamine in the preparation of animal feed additives, aiming to enhance the nutritional value of animal feed by adding glutamine while promoting animal health. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 Schematic diagram of the effect of feed Ala-Gln on the intestinal tissue structure of juvenile largemouth bass observed by H&E and PAS staining in an embodiment of the present invention;

[0014] Figure 2 Schematic diagram of the effect of feed Ala-Gln on the intestinal tissue structure of juvenile largemouth bass observed by transmission electron microscopy in an embodiment of the present invention (MV is microvilli, MC is mitochondria, GC is goblet cells, N is nucleus, and ER is endoplasmic reticulum). DETAILED DESCRIPTION

[0015] The following is combined with Figure 1-2 The present invention is described in further detail.

[0016] Example: Experimental application of glutamine in the treatment of soybean meal enteritis in largemouth bass

[0017] In this embodiment, three kinds of isonitrogen and isolipid feeds were prepared, namely, FM (fish meal, positive control group), SBM50 (soybean meal replacing 50% of fish meal, negative control group), and SBM50+1% AG (soybean meal replacing 50% of fish meal with 1% alanyl-glutamine added, a feed substitute for glutamine); healthy largemouth bass fry with an initial body weight of about 10 g were selected, and the experiment was divided into 3 groups, each with 4 replicates, each with 25 fish, and the breeding experiment lasted for 8 weeks.

[0018] Experimental data:

[0019] The growth performance results showed that the final average weight, weight gain rate and specific growth rate of SBM50+1%AG were significantly higher than those of FM group and SBM50 group (P<0.05), as shown in Table 1:

[0020] Table 1 Effects of dietary Ala-Gln on growth performance of juvenile largemouth bass

[0021] project FM SBM50 SBM50+1%AG Initial average weight g 10.39±.051 10.42±.012 10.4±.038 Final average weight g <![CDATA[51.25±1.13 ab ]]> <![CDATA[48.92±0.45 a ]]> <![CDATA[53.91±0.85 b ]]> Weight gain% <![CDATA[393.16±10.6 b ]]> <![CDATA[369.53±4.12 a ]]> <![CDATA[418.25±8.69 b ]]> Specific growth rate % <![CDATA[2.85±0.04b ab ]]> <![CDATA[2.76±0.02 a ]]> <![CDATA[2.94±0.03 b ]]> Feed coefficient % 1.12±0.02 1.12±0.02 1.1±0.01

[0022] In Table 1, the values ​​with different superscript letters in the same row (mean ± SD, n = 4) were significantly different (P < 0.05).

[0023] H&E and PAS staining results are shown in Figure 1 As shown in the figure, the thickness of the muscular layer in the SBM50 and SBM50+1%AG groups was significantly lower than that in the FM group (P<0.05), and the height of the intestinal folds in the SBM50+1%AG group was significantly higher than that in the SBM50 group (P<0.05), but there was no significant difference compared with the FM group (P>0.05). However, there was no significant difference in the width of the folds among the groups (P>0.05).

[0024] Transmission electron microscopy results Figure 2 As shown, the SBM50 group had pathological phenomena such as loss of intestinal villi, nuclear condensation, dissolution of sticky granules in goblet cells causing goblet cell vacuolation, dissolution of mitochondrial matrix and double membrane, while the electron microscopy results of the FM group and SBM+1%AG showed that the cell organelles were relatively normal.

[0025] This specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed. However, as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. A feed additive, characterized in that: The feed additive comprises glutamine, and is used for improving the nutritional value of animal feed and the health status of animals.

2. An application of glutamine in the preparation of animal feed additives.

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