A growth-promoting fermented feed additive and preparation method thereof

Abstract

The present invention belongs to the field of feed additive technology, and in particular to a fermented feed additive for promoting growth and a preparation method thereof. The additive includes the following ingredients in parts by weight: 10-20 parts of microbial fermentation bacteria liquid, 1-5 parts of modified montmorillonite, 5-10 parts of chlorella extract, and 0.5-1 parts of chicken bone powder; the modified montmorillonite preparation process is as follows: (1) montmorillonite is added to a nitric acid solution and heated and stirred for a period of time, and pretreated montmorillonite is obtained by filtration, washing, and calcination; (2) sodium carbonate, ferric nitrate, and calcium nitrate are added to water, heated and stirred to obtain a mixed solution; (3) the montmorillonite pretreated in step (1) is dispersed in water, the mixed solution of step (2) is added, heated and reacted, and modified montmorillonite is obtained. The additive of the present invention can not only enhance the activity of fermented strains, regulate intestinal microecological balance, but also meet the various nutritional needs during animal growth and promote animal growth performance.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and particularly relates to a growth-promoting fermented feed additive and a preparation method thereof. Background Art

[0002] In modern animal husbandry, feed additives are crucial for improving animal performance and health, playing a crucial role in promoting animal growth and well-being. With technological advancements and increasing consumer awareness of food safety and quality, the development of efficient, environmentally friendly, and safe feed additives has become a research hotspot. Fermented feed additives, with their unique microbial active ingredients and prebiotic effects, demonstrate significant advantages in promoting intestinal health, improving feed conversion rates, and reducing antibiotic use, garnering widespread attention and application.

[0003] Fermented feed additives typically contain one or more probiotics, which produce beneficial metabolites such as organic acids, enzymes, and vitamins through fermentation. These substances help improve the balance of the animal's intestinal microecology, increase feed digestibility, and promote healthy animal growth. However, existing fermented feed additives still have room for improvement in maintaining the activity of the fermented strains and promoting animal growth. Ensuring the high efficiency and stability of the fermented strains while maximizing their effectiveness in promoting animal growth is a major challenge, especially after long-term storage.

[0004] Montmorillonite is a natural layered silicate mineral that is widely used in various fields due to its unique physical and chemical properties, such as high specific surface area, good adsorption properties, and cation exchange capacity. For example, in agriculture, montmorillonite is often used as a soil conditioner, promoting crop growth by regulating soil structure and improving soil water and fertilizer retention. However, when directly applied as a feed additive, its effect is relatively limited, mainly reflected in its adsorption of nutrients in feed, and lacks specific functional optimization for animal growth needs.

[0005] Therefore, there is an urgent need to provide a growth-promoting fermented feed additive to solve the above problems. Summary of the Invention

[0006] In order to overcome the deficiencies of the prior art, the primary purpose of the present invention is to provide a growth-promoting fermented feed additive.

[0007] Another object of the present invention is to provide a method for preparing a growth-promoting fermented feed additive.

[0008] The purpose of the present invention is achieved through the following technical solutions:

[0009] A growth-promoting fermented feed additive comprising the following ingredients in parts by weight: 10-20 parts of microbial fermentation liquid, 1-5 parts of modified montmorillonite, 5-10 parts of chlorella extract, and 0.5-1 part of chicken bone powder;

[0010] The preparation process of the modified montmorillonite is as follows:

[0011] (1) adding montmorillonite to a nitric acid solution, heating and stirring for a period of time, filtering, washing, and calcining to obtain pretreated montmorillonite;

[0012] (2) adding sodium carbonate, ferric nitrate and calcium nitrate into water, heating and stirring to obtain a mixed solution;

[0013] (3) Dispersing the montmorillonite pretreated in step (1) in water to uniformly disperse the montmorillonite, then adding the mixed solution in step (2), heating and reacting to obtain modified montmorillonite.

[0014] Furthermore, the preparation process of the microbial fermentation broth is as follows: using water extract residue of Rhus chinensis leaves, water extract residue of Pueraria lobata and cooked potatoes as fermentation medium, and inoculating Bacillus subtilis liquid and Saccharomyces cerevisiae liquid as fermentation seed liquid into the fermentation medium for fermentation to obtain microbial fermentation broth.

[0015] Furthermore, the concentration of the nitric acid solution in step (1) is 3-7 mol / L; the material-liquid ratio of the montmorillonite to the nitric acid solution is 1 g:(9-11) mL; the heating and stirring temperature is 70-80° C., and the time is 5-6 h.

[0016] Furthermore, the calcination temperature in step (1) is 500-600° C. and the calcination time is 2-3 hours.

[0017] Furthermore, the mass ratio of the sodium carbonate, ferric nitrate, calcium nitrate and water in step (2) is (15-30):(35-55):(5-15):(800-1100).

[0018] Furthermore, the heating and stirring in step (2) is performed at a temperature of 40-50° C. and for a time of 10-14 h.

[0019] Furthermore, the material-liquid ratio of the pretreated montmorillonite and water in step (3) is 1:(8-10); the mass ratio of the pretreated montmorillonite to the sodium carbonate in the mixed solution of step (2) is 1:(0.5-1); the temperature of the heating reaction is 70-90°C and the time is 5-7h.

[0020] Furthermore, the mass ratio of the water extract of Rhus chinensis leaves, the water extract of Pueraria root and the cooked potatoes is 1:(2-5):(1-2).

[0021] Furthermore, the inoculation amount of the fermentation seed liquid is 1-5%; the concentrations of the Bacillus subtilis liquid and the Saccharomyces cerevisiae liquid are both 2×10 9 -5×10 9 CFU / mL; the fermentation temperature is 32-37°C and the fermentation time is 32-48h.

[0022] Another object of the present invention is achieved through the following technical solutions:

[0023] The preparation method of the above-mentioned growth-promoting fermented feed additive comprises the following steps:

[0024] The microbial fermentation broth, modified montmorillonite, chlorella extract, and chicken bone meal are weighed according to the above-mentioned parts by weight and mixed evenly to obtain the growth-promoting fermented feed additive.

[0025] The present invention has the following effects compared to the prior art:

[0026] 1. The growth-promoting fermented feed additive provided by the present invention is added with modified montmorillonite, which is obtained by subjecting the montmorillonite to acid treatment to increase the void structure, increase the specific surface area, and improve its adsorption capacity, and then adsorbing iron ions and calcium ions on the acid-treated montmorillonite, and performing pillar modification on its internal structure. The modified montmorillonite can not only provide trace elements for the growth of animals, but also adsorb toxins of harmful bacteria in the animal's intestines, improve the intestinal microenvironment, and help to increase the growth rate and weight of animals. On the other hand, the modified montmorillonite utilizes its pore structure to adsorb Bacillus subtilis and Saccharomyces cerevisiae in the fermentation broth, which can significantly improve the stability of the fermentation strains in the feed additive, thereby improving the utilization rate of the bacteria.

[0027] 2. The culture medium of the microbial fermentation liquid of the present invention is added with water extract of Rhus chinensis leaves and water extract of Pueraria lobata root. The two act synergistically in a specific proportion, so that the growth of the strain is better, thereby exerting the probiotic effect of the strain and promoting animal growth. DETAILED DESCRIPTION

[0028] The present invention will be described in further detail below in conjunction with the examples, but embodiments of the present invention are not limited thereto. Unless otherwise stated, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art. The test methods for which specific experimental conditions are not specified in the following examples are usually based on conventional experimental conditions or the experimental conditions recommended by the manufacturer. Unless otherwise stated, the reagents and raw materials used in the present invention can be obtained commercially.

[0029] The Bacillus subtilis in the present invention is an existing strain with a preservation number of CICC10071 and is commercially available; the Saccharomyces cerevisiae is an existing strain with a preservation number of CICC 30196 and is commercially available.

[0030] The preparation method of the water-extracted residue of Rhus chinensis leaves in the following examples is as follows: the Rhus chinensis leaves are dried, sterilized at 120° C. for 10 minutes, then pulverized, and screened through a 30-mesh sieve. Then, water equivalent to 15 times the weight of the Rhus chinensis leaves is added, and reflux extraction is performed twice, each extraction time being 2 hours. After the extraction is completed, the extract is filtered off, and the remaining residue phase is the desired Rhus chinensis leaf water-extracted residue.

[0031] The kudzu root water extract residue is also prepared by adopting the above scheme.

[0032] The preparation method of the chlorella extract comprises: adding chlorella powder, cellulase, and pectinase to water, wherein the mass ratio of chlorella powder, cellulase, pectinase, and water is 1:0.05:0.02:7. Enzymatic hydrolysis is performed at 48°C for 10 hours to obtain an enzymatic hydrolyzate, which is then filtered through an ultrafiltration membrane to collect the retentate. The retentate is then eluted with ethanol and deionized water, and dried to obtain the chlorella extract.

[0033] Example 1

[0034] A growth-promoting fermented feed additive comprising the following ingredients in parts by weight: 15 parts of microbial fermentation broth, 3 parts of modified montmorillonite, 7.5 parts of chlorella extract, and 0.75 parts of chicken bone meal;

[0035] The preparation process of the modified montmorillonite is as follows:

[0036] (1) adding montmorillonite to a 3 mol / L nitric acid solution, wherein the material-liquid ratio of the montmorillonite to the nitric acid solution is 1 g:10 mL; heating to 75° C., stirring and reacting for 5.5 hours, then filtering and collecting the solid, washing and drying it, and calcining it at 550° C. for 2.5 hours to obtain pretreated montmorillonite;

[0037] (2) Sodium carbonate, ferric nitrate, calcium nitrate, and water were weighed in a mass ratio of 25:45:10:1000, and then sodium carbonate, ferric nitrate, and calcium nitrate were added to water, heated and stirred at 45° C. for 12 h to obtain a mixed solution;

[0038] (3) dispersing the montmorillonite pretreated in step (1) in water, and then adding the mixed solution of step (2) thereto, wherein the material-liquid ratio of the pretreated montmorillonite to water is 1:9, and the mass ratio of the pretreated montmorillonite to the sodium carbonate in the mixed solution of step (2) is 1:0.75; heating the reaction at 80° C. for 6 h. After the reaction is completed, the reaction solution is allowed to stand at room temperature for 24 h, and then filtered, washed, and dried to obtain modified montmorillonite.

[0039] The preparation process of the microbial fermentation broth is as follows:

[0040] The water extract of Rhus chinensis leaves, the water extract of Pueraria root and cooked potatoes were mixed in a mass ratio of 1:4:1.5, and then sterile water was added in a material-liquid ratio of 4:1 and stirred to obtain a fermentation medium; the Bacillus subtilis liquid (3.0×10 9 CFU / mL), Saccharomyces cerevisiae culture (3.0×10 9 The seed fermentation liquid was inoculated into the fermentation medium at an inoculum rate of 3%, and fermented at 35°C for 40h to obtain the microbial fermentation liquid.

[0041] This embodiment also provides a method for preparing the above-mentioned growth-promoting fermented feed additive, comprising the following steps:

[0042] The microbial fermentation broth, modified montmorillonite, chlorella extract, and chicken bone meal are weighed according to the above-mentioned parts by weight and mixed evenly to obtain the growth-promoting fermented feed additive.

[0043] Example 2

[0044] A growth-promoting fermented feed additive, comprising the following ingredients in parts by weight: 10 parts of microbial fermentation liquid, 1 part of modified montmorillonite, 5 parts of chlorella extract, and 0.5 parts of chicken bone powder;

[0045] The preparation process of the modified montmorillonite is as follows:

[0046] (1) adding montmorillonite to a 5 mol / L nitric acid solution at a material-liquid ratio of 1 g:10 mL; heating to 70° C., stirring and reacting for 5 h, then filtering and collecting the solid, washing and drying it, and calcining it at 500° C. for 2 h to obtain pretreated montmorillonite;

[0047] (2) Sodium carbonate, ferric nitrate, calcium nitrate, and water were weighed in a mass ratio of 15:35:5:800, and then sodium carbonate, ferric nitrate, and calcium nitrate were added to water, heated and stirred at 40° C. for 10 h to obtain a mixed solution;

[0048] (3) dispersing the montmorillonite pretreated in step (1) in water, and then adding the mixed solution of step (2) thereto, wherein the material-liquid ratio of the pretreated montmorillonite to water is 1:8, and the mass ratio of the pretreated montmorillonite to the sodium carbonate in the mixed solution of step (2) is 1:0.5; heating the reaction at 70° C. for 7 h. After the reaction is completed, the reaction solution is allowed to stand at room temperature for 24 h, and then filtered, washed, and dried to obtain modified montmorillonite.

[0049] The preparation process of the microbial fermentation broth is as follows:

[0050] The water extract of Rhus chinensis leaves, the water extract of Pueraria root and cooked potatoes were mixed in a mass ratio of 1:2:1, and then sterile water was added in a material-liquid ratio of 4:1 and stirred to obtain a fermentation medium; the Bacillus subtilis liquid (2.0×10 9 CFU / mL), Saccharomyces cerevisiae culture (2.0×10 9 The mixture was used as a fermentation seed solution, inoculated into a fermentation medium at a rate of 5%, and fermented at 32°C for 48h to obtain a microbial fermentation solution.

[0051] This embodiment also provides a method for preparing the above-mentioned growth-promoting fermented feed additive, comprising the following steps:

[0052] The microbial fermentation broth, modified montmorillonite, chlorella extract, and chicken bone meal are weighed according to the above-mentioned parts by weight and mixed evenly to obtain the growth-promoting fermented feed additive.

[0053] Example 3

[0054] A growth-promoting fermented feed additive, comprising the following ingredients in parts by weight: 20 parts of microbial fermentation liquid, 5 parts of modified montmorillonite, 10 parts of chlorella extract, and 1 part of chicken bone powder;

[0055] The preparation process of the modified montmorillonite is as follows:

[0056] (1) Montmorillonite was added to a 7 mol / L nitric acid solution, heated to 80°C, stirred and reacted for 6 hours, and then the solid was collected by filtration, washed and dried, and then calcined at 600°C for 2 hours to obtain pretreated montmorillonite;

[0057] (2) Sodium carbonate, ferric nitrate, calcium nitrate, and water were weighed in a mass ratio of 30:55:15:1100, and then sodium carbonate, ferric nitrate, and calcium nitrate were added to water, heated and stirred at 50° C. for 10 h to obtain a mixed solution;

[0058] (3) dispersing the montmorillonite pretreated in step (1) in water, and then adding the mixed solution of step (2) thereto, wherein the material-liquid ratio of the pretreated montmorillonite to water is 1:10, and the mass ratio of the pretreated montmorillonite to the sodium carbonate in the mixed solution of step (2) is 1:1; heating the reaction at 90° C. for 5 h. After the reaction is completed, the reaction solution is allowed to stand at room temperature for 24 h, and then filtered, washed, and dried to obtain modified montmorillonite.

[0059] The preparation process of the microbial fermentation broth is as follows:

[0060] The water extract of Rhus chinensis leaves, the water extract of Pueraria root and cooked potatoes were mixed in a mass ratio of 1:5:2, and then sterile water was added in a material-liquid ratio of 4:1 and stirred to obtain a fermentation medium; the Bacillus subtilis liquid (5.0×10 9 CFU / mL), Saccharomyces cerevisiae culture (5.0×10 9 The mixture was used as a fermentation seed solution, inoculated into the fermentation medium at a rate of 1%, and fermented at 37°C for 32 h to obtain a microbial fermentation solution.

[0061] This embodiment provides a method for preparing a growth-promoting fermented feed additive, comprising the following steps:

[0062] The microbial fermentation broth, modified montmorillonite, chlorella extract, and chicken bone meal are weighed according to the above-mentioned parts by weight and mixed evenly to obtain the growth-promoting fermented feed additive.

[0063] Comparative Example 1

[0064] The difference between Comparative Example 1 and Example 1 is that the modified montmorillonite is replaced by montmorillonite, and the other contents are the same as those in Example 1.

[0065] Comparative Example 2

[0066] The difference between Comparative Example 2 and Example 1 is that when preparing the microbial fermentation broth, the Pueraria root water extract residue is omitted from the fermentation medium, and the ratio of the Rhus chinensis leaf water extract residue to the cooked potatoes is adjusted to 5:1; the rest is consistent with Example 1.

[0067] Comparative Example 3

[0068] The difference between this comparative example 3 and Example 1 is that: when preparing the microbial fermentation broth, the water extract of Rhus chinensis leaves is omitted from the fermentation medium, and the ratio of the water extract of Pueraria lobata to the cooked potatoes is adjusted to 5:1; the other contents are consistent with Example 1.

[0069] Comparative Example 4

[0070] The difference between Comparative Example 4 and Example 1 is that when preparing the microbial fermentation broth, the mass ratio of the water extract of Rhus chinensis leaves, the water extract of Pueraria root, and the cooked potatoes is adjusted to 2:1:1, and the rest is consistent with Example 1.

[0071] Test Example 1

[0072] In order to verify the activity of the bacteria in the fermented feed additives prepared in Examples 1-3 and Comparative Examples 1-4 of the present invention, the following method was used to investigate: the fermented feed additives prepared in the above groups were stored at room temperature for three months, added to 0.9% physiological saline, and filtered to obtain a test solution; a feed additive without adding microbial fermentation solution was set as a blank control group, and the absorbance value of each test solution in each group at a wavelength of 600 nm was detected by a microplate reader. The specific results are recorded in Table 1.

[0073] Table 1 Activity results of bacteria in each group of fermented feed additives

[0074] Group Before storage Three months later Example 1 group 1.52 1.38 Example 2 group 1.45 1.29 Example 3 group 1.49 1.34 Comparative Example 1 1.48 0.96 Comparative Example 2 1.17 1.02 Comparative Example 3 1.22 1.09 Comparative Example 4 1.39 1.25 Blank control group 0.56 0.57

[0075] From the experimental results in Table 1, it can be seen that the absorbance values of Examples 1-3 groups are significantly higher than those of Comparative Examples 1-4 groups, indicating that the strains in the fermented feed additives of Examples 1-3 groups have higher activity and better growth. Moreover, after three months of storage, the absorbance value of the strain did not decrease significantly, and the absorbance value of the strain was still 91% of the value before storage.

[0076] Modified montmorillonite is replaced by montmorillonite by comparative example 1 group, and compared with embodiment 1 group, after storage for three months, its absorbance value obviously reduces, and also the activity of bacterium in fermentation feed additive decreases, and it is seen that modified montmorillonite can improve the stability and activity of bacterium in feed additive.Compared to embodiment 1, comparative example 2-3 group adjusts the kind and dosage of radix kudzu chinensis water extract and rhus chinensis leaf water extract in fermentation medium, and absorbance value appears to be significantly reduced, and this shows that rhus chinensis leaf water extract and rhus chinensis leaf water extract can synergize, promote the growth of bacterial species, and promote the strain activity of bacillus subtilis and saccharomyces cerevisiae bacterial species.Comparative example 4 group adjusts the ratio of rhus chinensis leaf water extract, rhus chinensis leaf water extract in fermentation medium, and absorbance value also reduces, and shows that when adding the rhus chinensis leaf water extract, rhus chinensis leaf water extract of specific ratio in fermentation medium, the growth situation of bacillus subtilis and saccharomyces cerevisiae bacterial species is better, and strain activity is better.

[0077] Test Example 2

[0078] (1) A total of 56 healthy fattening pigs weighing about 80 kg were selected from a local farm and randomly divided into 8 groups, with 7 pigs in each group. Among them, the Example 1-3 groups and the Comparative Example 1-4 groups were respectively supplemented with the feed additives of the corresponding groups in the corn-soybean meal type basic diet (the additive dosage was 0.8% of the corn-soybean meal type basic diet dosage), and the control group was not supplemented with the feed additive of the present invention in the corn-soybean meal type basic diet; during the feeding period, each fattening pig was allowed to drink water freely, and the daily feed intake and the changes in the weight of the fattening pigs after feeding for 30 days were recorded, and the average feed-to-weight ratio was calculated. The relevant test results are shown in Table 2.

[0079] (2) After 30 days of feeding, one fattening pig of medium weight was selected from each replicate and slaughtered. The live weight, carcass weight, and dressing percentage of the fattening pigs from Examples 1 to 3 and Comparative Examples 1 to 4 were measured, where dressing percentage (%) = (carcass weight / live weight before slaughter) × 100%. The specific test results are recorded in Table 3.

[0080] Table 2 Effects of feed additives on growth performance of finishing pigs

[0081]

[0082] From the experimental results in Table 2, it can be seen that the average daily weight gain and feed intake of the fattening pigs in Example 1-3 groups are higher than those in Comparative Example 1-4 groups.

[0083] Compared with Example 1, the feed additive in Comparative Example 1 contained unmodified montmorillonite. The daily weight gain and feed intake of the fattening pigs in Comparative Example 1 were both lower than those in Example 1. This shows that modified montmorillonite plays an important role in improving the daily weight gain and feed intake of animals. On the one hand, the iron and calcium ions adsorbed in the pores of the modified montmorillonite of the present invention can provide essential trace elements for animal growth. In addition, the modified montmorillonite also has the function of adsorbing harmful bacteria and toxins in the animal's intestines and regulating the intestines of fattening pigs, which helps to increase the growth rate and weight of the animals. On the other hand, the modified montmorillonite utilizes its pore structure to adsorb Bacillus subtilis and Saccharomyces cerevisiae in the fermentation broth, which can improve the stability and activity of the fermentation strains in the feed additive, thereby increasing the utilization rate of the bacteria, giving full play to the beneficial effects of the bacteria, and promoting animal growth.

[0084] Compared with the Example 1 group, the preparation method of the fermentation broth was adjusted in the Comparative Examples 2-4 groups, and the daily weight gain and feed intake of the fattening pigs also showed varying degrees of decrease. Combined with the results of the bacterial activity of Test Example 1, it can be seen that the water extract of Rhus chinensis leaves and the water extract of Pueraria lobata in the fermentation medium act synergistically at a specific ratio to enhance the activity of Bacillus subtilis and Saccharomyces cerevisiae, thereby adjusting the intestinal health of the animals and promoting the growth of fattening pigs.

[0085] Table 3 Pre-slaughter live weight, carcass weight and slaughter rate of fattening pigs in each group

[0086]

[0087]

[0088] From the experimental results in Table 3, it can be seen that the slaughter rate of animals in Example 1-3 groups is significantly higher than that in Comparative Example 1-4 groups.

[0089] Finally, it should be noted that the above embodiments are intended only to illustrate the technical solutions of the present invention and are not intended to limit the same. The basic principles and main features of the present invention have been described above using specific implementation schemes. Modifications or substitutions may be made based on the present invention, but such modifications or substitutions do not detract from the essence of the corresponding technical solutions from the scope of protection claimed by the present invention.

Claims

1. A growth-promoting fermented feed additive, characterized in that: The invention comprises the following ingredients in parts by weight: 10-20 parts of microbial fermentation liquid, 1-5 parts of modified montmorillonite, 5-10 parts of chlorella extract, and 0.5-1 part of chicken bone powder; The preparation process of the modified montmorillonite is as follows: (1) adding montmorillonite to a nitric acid solution, heating and stirring, filtering, washing, and calcining to obtain pretreated montmorillonite; (2) Add sodium carbonate, ferric nitrate and calcium nitrate into water, heat and stir to obtain a mixed solution; (3) dispersing the montmorillonite pretreated in step (1) in water, then adding the mixed solution of step (2), heating and reacting to obtain modified montmorillonite; The preparation process of the microbial fermentation liquid is as follows: using water extract residue of Rhus chinensis leaves, water extract residue of Pueraria root and cooked potatoes as fermentation medium, using Bacillus subtilis liquid and Saccharomyces cerevisiae liquid as fermentation seed liquid to inoculate into the fermentation medium for fermentation, thereby obtaining the microbial fermentation liquid; The mass ratio of the water extract of Rhus chinensis leaves, the water extract of Pueraria root and the cooked potatoes is 1:(2-5):(1-2); The concentration of the nitric acid solution in step (1) is 3-7 mol / L; the material-liquid ratio of the montmorillonite to the nitric acid solution is 1 g:10 mL; the temperature of the heating and stirring in step (1) is 70-80° C. and the time is 5-6 h; the temperature of the calcination in step (1) is 500-600° C. and the time is 2-3 h; The mass ratio of sodium carbonate, ferric nitrate, calcium nitrate and water in step (2) is (15-30):(35-55):(5-15):(800-1100); the temperature of heating and stirring in step (2) is 40-50°C and the time is 10-14h; The material-liquid ratio of the pretreated montmorillonite and water in step (3) is 1:(8-10); the mass ratio of the pretreated montmorillonite to the sodium carbonate in the mixed solution of step (2) is 1:(0.5-1); the temperature of the heating reaction in step (3) is 70-90°C and the time is 5-7h; The inoculation amount of the fermentation seed liquid is 1-5%; the concentrations of the Bacillus subtilis liquid and the Saccharomyces cerevisiae liquid are both 2×10 9 -5×10 9 CFU / mL.

2. A growth-promoting fermented feed additive according to claim 1, characterized in that: The fermentation temperature is 32-37° C. and the fermentation time is 32-48 hours.

3. The method for preparing the growth-promoting fermented feed additive according to claim 1, characterized in that: The method comprises the following steps: weighing microbial fermentation broth, modified montmorillonite, chlorella extract, and chicken bone powder according to the weight proportions according to claim 1 and mixing them evenly to obtain the growth-promoting fermented feed additive.

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