Skin-moistening hand-washing-free gel disinfectant and preparation method thereof

Abstract

The invention relates to the field of disinfectant preparation, and particularly discloses a skin-moistening hand-washing-free gel disinfectant and a preparation method thereof. The gel disinfectant is prepared from the following components in parts by weight: 1.5 to 2.5 parts of chlorhexidine gluconate, 65 to 75 parts of ethanol, 10 to 15 parts of carbomer and 2 to 5 parts of skin moistening penetrant. The gel disinfectant provided by the invention has the advantage of long-acting antibacterial effect; in addition, the preparation method provided by the invention has the advantage of simple process.

Description

Technical Field

[0001] The present application relates to the field of disinfectant preparation, and more specifically, to a moisturizing hand-free gel disinfectant and a preparation method thereof. Background Art

[0002] Disinfectant is a liquid disinfectant that is characterized by strong bactericidal ability and fast action. Disinfectant gel is a hand sanitizer that can eliminate viruses and bacteria after use to achieve the purpose of disinfection.

[0003] The main ingredient of the commonly used hand-free disinfectant currently is ethanol, but the durability of this type of hand-free disinfectant is poor. Summary of the invention

[0004] In order to improve the durability of hand-free gel disinfectant, the present application provides a moisturizing hand-free gel disinfectant and a preparation method thereof.

[0005] In the first aspect, the present application provides a moisturizing hand-free gel disinfectant, which adopts the following technical solution: A skin-moistening hand-free gel disinfectant comprises the following components in parts by weight: 1.5 to 2.5 parts of chlorhexidine gluconate, 65 to 75 parts of ethanol, 10 to 15 parts of carbomer and 2 to 5 parts of a skin-moistening penetrant.

[0006] By adopting the above technical scheme, the bacterial cell membrane is mainly composed of a phospholipid bilayer and proteins. Since the phospholipid functional groups in the outer layer of the cell membrane are negatively charged, the chlorhexidine molecules as cations can easily interact with and adsorb the phospholipid molecules on the cell membrane as targets, thereby causing rupture and damage to the cell membranes of various microorganisms, causing the cell contents to leak out and kill the bacteria. When other organic matter exists on the skin surface, the bactericidal effect of chlorhexidine is not affected. In addition, since chlorhexidine has good affinity with the skin, it is easy to adsorb and remain on the skin surface, thereby achieving a long-lasting antibacterial effect on the skin surface, that is, improving the durability of the hand-free gel disinfectant.

[0007] Preferably, the emollient penetrant comprises allantoin.

[0008] By adopting the above technical solution, allantoin can keep the skin hydrated, moisturized and soft. At the same time, the addition of allantoin can protect the tissue while absorbing water and preventing water evaporation to keep the skin moist. In addition, allantoin has a bactericidal and preservative effect, thereby further improving the bactericidal effect of the disinfectant.

[0009] Preferably, the emollient penetrant comprises allantoin and a carrier, and the weight ratio of the allantoin to the carrier is 1:(0.4-1).

[0010] By adopting the above technical solution, the addition of the carrier can cause allantoin to be slowly released, thereby improving the moisturizing effect of the disinfectant and extending the bactericidal effect of the disinfectant.

[0011] Preferably, the weight ratio of allantoin to the carrier is 1:(0.4-0.7).

[0012] Preferably, the carrier is a protein / polysaccharide hydrogel.

[0013] By adopting the above technical solution, the carrier adopts protein / polysaccharide hydrogel, so that after the staff uses the disinfectant, the protein / polysaccharide hydrogel can adhere to the skin surface, thereby forming a film, so that bacteria and the like are not easy to contact the skin surface, thereby further extending the bactericidal effect of the disinfectant.

[0014] Preferably, the emollient penetrant is obtained by dissolving allantoin in water to obtain a mixture, and then mixing the mixture with a carrier and drying the mixture.

[0015] By adopting the above technical solution, the preparation process of the emollient penetrant is simple.

[0016] Preferably, the gel disinfectant also includes 4 to 7 parts of plant extracts, the plant extracts include Phellodendron chinense bark extract, Paeonia suffruticosa root bark extract and Gentiana scabra root extract, and the mass ratio of Phellodendron chinense bark extract, Paeonia suffruticosa root bark extract and Gentiana scabra root extract is 1:(0.6-1):(1-1.5).

[0017] By adopting the above technical scheme, using gentian root extract, peony root bark extract, and phellodendron bark extract as main ingredients and adding them to the gel disinfectant, it not only helps to improve the skin's moisturizing properties, but also can enhance the skin's own defense, and has a certain effect of clearing some inflammatory mediators caused by ultraviolet radiation or skin dryness, thereby achieving soothing and repairing effects.

[0018] In a second aspect, the present application provides a method for preparing a moisturizing hand-free gel disinfectant, using the following technical solution: A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1. Weigh each substance according to weight; S2. Evenly mix chlorhexidine gluconate and water, and then evenly mix with other substances to obtain a moisturizing hand-free gel disinfectant.

[0019] By adopting the above technical solution, the preparation method of the present application has the advantage of simple process.

[0020] In summary, this application has the following beneficial effects: 1. The cell membrane of bacteria is mainly composed of a phospholipid bilayer and proteins. Since the phospholipid functional groups on the outer layer of the cell membrane are negatively charged, the chlorhexidine molecules as cations can easily interact with and adsorb the phospholipid molecules on the cell membrane as targets, thereby causing rupture and damage to the cell membranes of various microorganisms, allowing the cell contents to leak out and kill the bacteria. When other organic matter exists on the skin surface, the bactericidal effect of chlorhexidine is not affected. In addition, since chlorhexidine has good affinity with the skin, it is easy to adsorb and remain on the skin surface, thereby achieving a long-lasting antibacterial effect on the skin surface.

[0021] 2. In the present application, the carrier preferably adopts protein / polysaccharide hydrogel, so that after the staff uses the disinfectant, the protein / polysaccharide hydrogel can adhere to the skin surface, thereby forming a film, so that bacteria and the like are not easy to contact the skin surface, thereby further extending the bactericidal effect of the disinfectant.

[0022] 3. The method of the present application has the advantage of simple process. DETAILED DESCRIPTION

[0023] The present application is further described in detail below in conjunction with comparative examples and embodiments.

[0024] The raw materials used in the preparation examples and embodiments of the present application are all commercially available, including: hyaluronic acid was purchased from Sinopharm Chemical Reagent Co., Ltd., chlorella protein was purchased from Tianjin Damao Chemical Reagent Factory, hydroxysuccinimide was purchased from Shandong Jinyufeng New Materials Co., Ltd., carbomer was purchased from Carbomer 940 of Desheng Materials, gentian root extract was purchased from Xi'an Green Biotechnology Co., Ltd., peony root bark extract was purchased from Shandong Shoucheng Chemical Co., Ltd., and Phellodendron chinense bark extract was purchased from Ningxia Vanilla Biotechnology Co., Ltd.

[0025] Preparation Example Preparation Example 1 Preparation of a carrier: a certain amount of chlorella protein and hyaluronic acid are dispersed in deionized water to prepare a mixed solution with a mass concentration of 10%, wherein the mass ratio of chlorella protein to hyaluronic acid is 3:1; after the mixed solution is mixed evenly, hydroxysuccinimide is added, and the mass ratio of hydroxysuccinimide to the mixed solution is 3:10, and then 1-ethyl-(3-dimethylaminopropyl) carbodiimide is slowly poured and stirred evenly to obtain a mixture, wherein: the molar ratio of hydroxysuccinimide to 1-ethyl-(3-dimethylaminopropyl) carbodiimide is 1:1; the mixture is freeze-dried at 4°C to obtain a protein / polysaccharide hydrogel.

[0026] Preparation Example 2 Preparation of a skin moisturizing penetrant: Dissolve allantoin in deionized water to obtain a solution, add the protein / polysaccharide hydrogel prepared in Preparation Example 1 to the solution and mix well to obtain a mixed solution, and then dry the mixed solution at room temperature to obtain a skin moisturizing penetrant, wherein the mass ratio of allantoin to protein / polysaccharide hydrogel is 1:0.4.

[0027] Preparation Example 3 Preparation of a skin moisturizing penetrant: Dissolve allantoin in deionized water to obtain a solution, add the protein / polysaccharide hydrogel prepared in Preparation Example 1 to the solution and mix well to obtain a mixed solution, and then dry the mixed solution at room temperature to obtain a skin moisturizing penetrant, wherein the mass ratio of allantoin to protein / polysaccharide hydrogel is 1:0.7.

[0028] Preparation Example 4 Preparation of a skin moisturizing penetrant: Dissolve allantoin in deionized water to obtain a solution, add the protein / polysaccharide hydrogel prepared in Preparation Example 1 to the solution and mix evenly to obtain a mixed solution, and then dry the mixed solution at room temperature to obtain a skin moisturizing penetrant, wherein the mass ratio of allantoin to the protein / polysaccharide hydrogel is 1:1.

[0029] Preparation Example 5 Preparation of the skin-moistening penetrant: dissolving allantoin in deionized water to obtain a solution, adding gelatin to the solution and mixing evenly to obtain a mixed solution, and then drying the mixed solution at room temperature to obtain the skin-moistening penetrant, wherein the mass ratio of allantoin to gelatin is 1:0.7.

[0030] Preparation Example 6 Preparation of plant extract: 1 kg of Phellodendron amurense bark extract, 0.6 kg of Paeonia suffruticosa root bark extract and 1 kg of Gentiana scabra extract were mixed uniformly to obtain the plant extract.

[0031] Preparation Example 7 Preparation of plant extract: 1 kg of Phellodendron amurense bark extract, 1 kg of Paeonia suffruticosa root bark extract and 1.5 kg of Gentiana scabra extract were mixed evenly to obtain the plant extract.

[0032] Preparation Example 8 Preparation of plant extract: 1 kg of Phellodendron amurense bark extract, 0.8 kg of Paeonia suffruticosa root bark extract and 1.3 kg of Gentiana scabra extract were mixed uniformly to obtain the plant extract. Example

[0033] Example 1 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 1.5 kg of chlorhexidine gluconate, 65 kg of ethanol, 10 kg of carbomer and 2 kg of a moisturizing penetrant are used, wherein the moisturizing penetrant is allantoin; S2. Evenly mix chlorhexidine gluconate and water, and then evenly mix with other substances to obtain a moisturizing hand-free gel disinfectant.

[0034] Example 2 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 2.5 kg of chlorhexidine gluconate, 75 kg of ethanol, 15 kg of carbomer and 5 kg of a moisturizing penetrant are used, wherein the moisturizing penetrant is allantoin; S2. Evenly mix chlorhexidine gluconate and water, and then evenly mix with other substances to obtain a moisturizing hand-free gel disinfectant.

[0035] Example 3 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 2 kg of chlorhexidine gluconate, 70 kg of ethanol, 13 kg of carbomer and 3 kg of a moisturizing penetrant, wherein the moisturizing penetrant is allantoin; S2. Evenly mix chlorhexidine gluconate and water, and then evenly mix with other substances to obtain a moisturizing hand-free gel disinfectant.

[0036] Example 4 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 2 kg of chlorhexidine gluconate, 70 kg of ethanol, 13 kg of carbomer and 3 kg of a moisturizing penetrant, wherein the moisturizing penetrant is the moisturizing penetrant prepared in Preparation Example 2; S2. Evenly mix chlorhexidine gluconate and water, and then evenly mix with other substances to obtain a moisturizing hand-free gel disinfectant.

[0037] Example 5 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 2 kg of chlorhexidine gluconate, 70 kg of ethanol, 13 kg of carbomer and 3 kg of a moisturizing penetrant, wherein the moisturizing penetrant is the moisturizing penetrant prepared in Preparation Example 3; S2. Evenly mix chlorhexidine gluconate and water, and then evenly mix with other substances to obtain a moisturizing hand-free gel disinfectant.

[0038] Example 6 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 2 kg of chlorhexidine gluconate, 70 kg of ethanol, 13 kg of carbomer and 3 kg of a moisturizing penetrant are used, wherein the moisturizing penetrant is the moisturizing penetrant prepared in Preparation Example 4; S2. Evenly mix chlorhexidine gluconate and water, and then evenly mix with other substances to obtain a moisturizing hand-free gel disinfectant.

[0039] Example 7 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 2 kg of chlorhexidine gluconate, 70 kg of ethanol, 13 kg of carbomer and 3 kg of a moisturizing penetrant, wherein the moisturizing penetrant is the moisturizing penetrant prepared in Preparation Example 5; S2. Evenly mix chlorhexidine gluconate and water, and then evenly mix with other substances to obtain a moisturizing hand-free gel disinfectant.

[0040] Example 8 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 2kg of chlorhexidine gluconate, 70kg of ethanol, 13kg of carbomer, 3kg of a moisturizing penetrant and 4kg of a plant extract, wherein the moisturizing penetrant is the moisturizing penetrant prepared in Preparation Example 4, and the plant extract is the plant extract prepared in Preparation Example 6; S2, uniformly mixing the chlorhexidine gluconate and water, and then uniformly mixing with other substances to obtain a moisturizing hand-free gel disinfectant.

[0041] Example 9 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 2kg of chlorhexidine gluconate, 70kg of ethanol, 13kg of carbomer, 3kg of a moisturizing penetrant and 7kg of a plant extract, wherein the moisturizing penetrant is the moisturizing penetrant prepared in Preparation Example 4, and the plant extract is the plant extract prepared in Preparation Example 6; S2, uniformly mixing the chlorhexidine gluconate and water, and then uniformly mixing with other substances to obtain a moisturizing hand-free gel disinfectant.

[0042] Example 10 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 2kg of chlorhexidine gluconate, 70kg of ethanol, 13kg of carbomer, 3kg of a moisturizing penetrant and 5kg of a plant extract, wherein the moisturizing penetrant is the moisturizing penetrant prepared in Preparation Example 4, and the plant extract is the plant extract prepared in Preparation Example 6; S2, uniformly mixing the chlorhexidine gluconate and water, and then uniformly mixing with other substances to obtain a moisturizing hand-free gel disinfectant.

[0043] Embodiment 11 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 2kg of chlorhexidine gluconate, 70kg of ethanol, 13kg of carbomer, 3kg of a moisturizing penetrant and 5kg of a plant extract, wherein the moisturizing penetrant is the moisturizing penetrant prepared in Preparation Example 4, and the plant extract is the plant extract prepared in Preparation Example 7; S2, uniformly mixing the chlorhexidine gluconate and water, and then uniformly mixing with other substances to obtain a moisturizing hand-free gel disinfectant.

[0044] Example 12 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 2kg of chlorhexidine gluconate, 70kg of ethanol, 13kg of carbomer, 3kg of a moisturizing penetrant and 5kg of a plant extract, wherein the moisturizing penetrant is the moisturizing penetrant prepared in Preparation Example 4, and the plant extract is the plant extract prepared in Preparation Example 8; S2, uniformly mixing the chlorhexidine gluconate and water, and then uniformly mixing with other substances to obtain a moisturizing hand-free gel disinfectant.

[0045] Comparative Example Comparative Example 1 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 1.5 kg of chlorhexidine gluconate, 65 kg of ethanol and 10 kg of carbomer; S2. Evenly mix chlorhexidine gluconate and water, and then evenly mix with other substances to obtain a moisturizing hand-free gel disinfectant.

[0046] Comparative Example 2 A method for preparing a moisturizing hand-free gel disinfectant comprises the following steps: S1, weighing various substances according to weight, wherein 66.5 kg of ethanol, 10 kg of carbomer and 2 kg of a moisturizing penetrant are taken, wherein the moisturizing penetrant is allantoin; S2. Evenly mix chlorhexidine gluconate and water, and then evenly mix with other substances to obtain a moisturizing hand-free gel disinfectant.

[0047] Performance testing Detection Methods 1. The sterilization rates of the hand-free disinfectant gel prepared in the examples and comparative examples against Staphylococcus aureus, Escherichia coli and Candida albicans were determined by referring to the sterilization method in Appendix C of GB15979-2002 "Hygiene Standards for Disposable Hygiene Products". See Table 1; 2. Persistent efficacy test: In order to verify the long-lasting antibacterial effect of the hand-free gel disinfectant in this application, an antibacterial test was conducted in part with reference to QB / T2738-2005 "Evaluation Method for Antibacterial and Antibacterial Effects of Daily Chemical Products". The details are as follows: (1) Using silicone gloves to simulate human hands, 14 pairs of silicone gloves were prepared, and the 14 pairs of gloves were set corresponding to Examples 1-12 and Comparative Examples 1-2. The silicone gloves were put on the hands, and the disinfectants obtained in Examples 1-12 and Comparative Examples 1-2 were used to rub the silicone gloves, respectively, in a manner that simulates the use of hand-free gel disinfectant, and the rubbing strength and time of the silicone gloves were consistent, and the silicone gloves were placed at room temperature for 2 hours; (2) The test bacteria used were Staphylococcus aureus ATCC6538, which was cultured for the third generation and diluted with tryptone soy broth to a concentration of 1×10 4 cfu / mL; (3) 25 μL of bacterial solution was dropped into an area with a diameter of 4 cm on the silicone glove. After 60 minutes, the colony count concentration of Staphylococcus aureus was tested to evaluate the antibacterial persistence. The test results are shown in Table 1.

[0048] 3. Emollient effect test: (1) 14 volunteers were recruited for testing, and the gel disinfectants prepared in Examples 1-12 and Comparative Examples 1-2 corresponded to the volunteers one by one; (2) Clean the inner forearms of both hands of the subject and mark 6 areas on the inner forearms of both sides, 3 areas on each arm, each area is 3*3cm, and the interval between areas is more than 1cm. One area without applying the sample serves as the blank control group, and the other two areas are applied with the corresponding gel disinfectant as the test group; (3) The test area of ​​the subject was exposed to a constant temperature and humidity environment for 20 min. The subject did not drink water or other beverages during this period. Both forearms were exposed and the subject remained relaxed. (4) After detecting the initial value of the stratum corneum moisture content in each area of ​​the skin, spray the disinfectant on the corresponding position; (5) 5 hours after applying the sample, measure the blank control sample and the sample area. Repeat the measurement 3 times for each area, calculate the average value, and record it in Table 1.

[0049] Table 1 It can be seen from Example 1 and Comparative Example 2 and Table 1 that the bactericidal effect of the disinfectant obtained by using ethanol and chlorhexidine gluconate is more significant than that of the disinfectant obtained by using ethanol alone.

[0050] Combining Example 6 and Example 7 with Table 1, it can be seen that when protein / polysaccharide hydrogel is used as a carrier, the moisture content of the stratum corneum increases significantly. The reason is that: the carrier uses protein / polysaccharide hydrogel, so that after the staff uses the disinfectant, the protein / polysaccharide hydrogel can adhere to the skin surface to form a film, which makes it difficult for moisture to be lost on the one hand, and the water-trapping property of the protein / polysaccharide hydrogel also has a hydrating effect on the skin; in addition, the film formed by the protein / polysaccharide hydrogel on the skin surface makes it difficult for bacteria to contact the skin surface, thereby further extending the bactericidal effect of the disinfectant.

[0051] Combining Example 7 and Example 8 with Table 1, it can be seen that the addition of plant extracts further increases the moisture content of the stratum corneum. The reason is that the addition of plant extracts improves the moisturizing properties of the skin, thereby reducing the loss of moisture on the skin after the use of the gel disinfectant.

[0052] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A moisturizing hand-free gel disinfectant, characterized in that: The invention comprises the following components in parts by weight: 1.5 to 2.5 parts of chlorhexidine gluconate, 65 to 75 parts of ethanol, 10 to 15 parts of carbomer and 2 to 5 parts of a skin moisturizing penetrant.

2. A moisturizing hand-free gel disinfectant according to claim 1, characterized in that: The emollient penetrant includes allantoin.

3. A moisturizing hand-free gel disinfectant according to claim 1, characterized in that: The skin moisturizing penetrant comprises allantoin and a carrier, and the weight ratio of the allantoin to the carrier is 1:(0.4-1).

4. A moisturizing hand-free gel disinfectant according to claim 1, characterized in that: The weight ratio of the allantoin to the carrier is 1:(0.4-0.7).

5. A moisturizing hand-free gel disinfectant according to claim 3, characterized in that: The carrier is a protein / polysaccharide hydrogel.

6. A moisturizing hand-free gel disinfectant according to claim 1, characterized in that: The emollient penetrant is obtained by dissolving allantoin in water to obtain a mixture, and then mixing the mixture with a carrier and drying the mixture.

7. A moisturizing hand-free gel disinfectant according to claim 1, characterized in that: The gel disinfectant also includes 4 to 7 parts of plant extracts, including Phellodendron amurense bark extract, Paeonia suffruticosa root bark extract and Gentiana scabra root extract, and the mass ratio of the Phellodendron amurense bark extract, Paeonia suffruticosa root bark extract and Gentiana scabra root extract is 1:(0.6-1):(1-1.5).

8. A method for preparing a moisturizing hand-free gel disinfectant, characterized in that: The method comprises the following steps: S1, weighing each substance according to weight; S2. Evenly mix chlorhexidine gluconate and water, and then evenly mix with other substances to obtain a moisturizing hand-free gel disinfectant.

Images