Droplet generation structure, microfluidic channel, microfluidic chip and droplet generation method

Abstract

The invention discloses a liquid drop generation structure, a micro-fluidic channel, a micro-fluidic chip and a liquid drop generation method. The liquid drop generating structure comprises a side wall, a deformable part and a driving part, the deformable part is connected with the side wall to form a channel, and the deformable part is used for controlling opening and closing of the channel through deformation of the deformable part; the driving part is connected with the deformable part and used for driving the deformable part to deform. The microfluidic flow channel comprises the liquid drop generation structure. The micro-fluidic chip comprises the micro-fluidic flow channel. The droplet generation method comprises the following steps: injecting liquid into the flow channel body through an inlet of the flow channel body; the deformable part is driven by the driving part to deform so as to close the channel, liquid in the flow channel body extrudes the deformable part under the action of driving force so that a thin seam channel can be formed between the deformable part and the side wall, and the liquid passes through the thin seam channel to form jet flow liquid drops to be jetted out.

Description

Technical Field

[0001] The present application relates to the field of microfluidics, and in particular to a droplet generation structure, a microfluidic channel, a microfluidic chip and a droplet generation method. Background Art

[0002] In some microfluidic applications, it is necessary to encapsulate single or multiple particles in a single droplet to form a research sample. For example, in biological research, a single droplet can be used to encapsulate single or multiple cells to form a biochemical reaction container suitable for high-throughput and high-resolution biochemical analysis.

[0003] In the related art, passive methods such as T-channel method, flow focusing method, coaxial flow focusing method, etc. are usually used to prepare droplets. The droplets generated by these methods include the following modes: absolutely unstable drip droplet generation mode, relatively unstable jet droplet generation mode and multiple critical transition state modes. Among them, the droplet uniformity of the drip droplet generation mode is good, but the flux is low; the droplet flux of the jet droplet generation mode is high, but the droplet uniformity is not very good. Therefore, there is an urgent need for a droplet generation solution that can meet both uniformity and high throughput. Summary of the invention

[0004] In order to solve at least one of the above technical problems, the present application provides a droplet generation structure, a microfluidic channel, a microfluidic chip and a droplet generation method, and the technical solutions adopted are as follows.

[0005] The present application provides a droplet generation structure, comprising: a side wall; a deformable member, the deformable member is connected to the side wall to form a channel, the deformable member is used to control the opening and closing of the channel by its own deformation; a driving member, the driving member is connected to the deformable member, and the driving member is used to drive the deformable member to deform.

[0006] According to the droplet generation structure provided by the present application, at least the following technical effects are achieved: the deformable member can control the closure of the channel by its own deformation, and the liquid can squeeze the deformable member under the action of the driving force, so that a thin slit channel is formed between the deformable member and the side wall, and the liquid passes through the thin slit channel to form jet droplets. In other words, the deformable member can form a valve that controls the opening and closing of the channel through its own deformation. When the valve is closed, the liquid can break through the valve under the action of a strong driving force and be ejected from the thin slit channel with almost no height in a very thin posture, thereby forming jet droplets. The liquid can form stable jet droplets of uniform size through the thin slit channel, and can also ensure a high flux of droplets.

[0007] In certain embodiments of the present application, the deformable part includes an elastic deformable membrane, two sides of which are respectively connected to two sides of the side wall, so that the elastic deformable membrane and the side wall are combined to form the channel, and when the elastic deformable membrane is deformed, it can bulge toward the side wall to fit the side wall.

[0008] In certain embodiments of the present application, the driving member includes a pressurized air chamber, one side of the elastic deformation membrane is located in the pressurized air chamber, and the pressurized air chamber drives the elastic deformation membrane to deform when pressurized.

[0009] In some embodiments of the present application, the top of the side wall is arc-shaped.

[0010] The present application also provides a microfluidic channel, which includes the above-mentioned droplet generation structure and a channel body having an inlet and an outlet, and the channel is connected to one end of the outlet of the channel body.

[0011] In certain embodiments of the present application, the microfluidic channel further includes a droplet collecting mechanism, and the droplet collecting mechanism is connected to an end of the droplet generating structure away from the channel body.

[0012] In certain embodiments of the present application, the liquid filled in the droplet collection mechanism is incompatible with the droplets.

[0013] The present application also provides a microfluidic chip, which includes the above-mentioned microfluidic channel, and also includes a first plate body and a second plate body, the first plate body is provided with a first groove, the second plate body is provided with a second groove, the driving member is arranged in the second groove, the deformable member is connected to the outer periphery of the opening of the second groove, the first plate body and the second plate body are covered and connected so that the first groove and the second plate body enclose the channel body, and the first groove and the deformable member enclose the channel.

[0014] The present application also provides a droplet generation method, comprising the following steps:

[0015] injecting liquid into the flow channel body through the inlet of the flow channel body;

[0016] The deformable member is driven to deform by a driving member to close the channel. The liquid in the flow channel body squeezes the deformable member under the action of the driving force to form a thin slit channel between the deformable member and the side wall. The liquid passes through the thin slit channel to form jet droplets and is ejected.

[0017] In certain embodiments of the present application, the driving member includes a pressurized air chamber, and the deformable member includes an elastic deformable membrane. The elastic deformable membrane is continuously loaded with air pressure through the pressurized air chamber to drive the elastic deformable membrane to deform. The air pressure of the pressurized air chamber is set to a constant pressure or a regularly high-frequency changing air pressure. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The aspects and advantages described and / or attached in the embodiments of the present application will become apparent and easy to understand in conjunction with the following drawings. It should be noted that the embodiments embodied in the following drawings are exemplary and are only used to explain the present application, and cannot be understood as limiting the present application.

[0019] Figure 1 A schematic structural diagram of a droplet generation structure (the deformable member is not deformed) provided in an embodiment of the present application;

[0020] Figure 2 A schematic diagram of the structure of a droplet generation structure (deformable member deforms) provided in an embodiment of the present application;

[0021] Figure 3 A schematic diagram of the structure of the microfluidic chip provided in the embodiment of the present application;

[0022] Figure 4 for Figure 3 Middle AA section view;

[0023] Figure 5 for Figure 4 Middle BB section view;

[0024] Figure 6 for Figure 3 Another cross-sectional view along the AA direction;

[0025] Figure 7 for Figure 6 Middle CC section view.

[0026] Reference numerals:

[0027] Droplet generation structure 110, side wall 111, deformable member 112, channel 113, driving member 114, flow channel body 120, vertical portion 121, inlet 1211, horizontal portion 122, plane side wall 1221, second arc-shaped side wall 1222, horizontal flow channel 1223, droplet collection mechanism 130, oil phase channel 131;

[0028] A first plate body 210 and a second plate body 220 . DETAILED DESCRIPTION

[0029] Combine the following Figures 1 to 7Embodiments of the present application are described in detail, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application, and cannot be understood as limiting the present application.

[0030] In the description of the present application, it should be understood that if the terms "center", "middle", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "axial", "radial", "circumferential" and the like appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. In addition, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present application, unless otherwise specified, "multiple" means two or more.

[0031] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0032] In some microfluidic applications, it is necessary to encapsulate single or multiple particles in a single droplet to form a research sample. For example, in biological research, a single droplet can be used to encapsulate single or multiple cells to form a biochemical reaction container suitable for high-throughput and high-resolution biochemical analysis.

[0033] In the related art, passive methods such as T-channel method, flow focusing method, coaxial flow focusing method, etc. are usually used to prepare droplets. The droplets generated by these methods include the following modes: absolutely unstable drip droplet generation mode, relatively unstable jet droplet generation mode and multiple critical transition state modes. Among them, the droplet uniformity of the drip droplet generation mode is good, but the flux is low; the droplet flux of the jet droplet generation mode is high, but the droplet uniformity is not very good.

[0034] See also Figure 1, the embodiment of the present application provides a droplet generation structure 110. Exemplarily, the droplet generation structure 110 includes a side wall 111, a deformable member 112 and a driving member 114, the deformable member 112 is connected to the side wall 111 to form a channel 113, and the deformable member 112 is used to control the opening and closing of the channel 113 by its own deformation; the driving member 114 is connected to the deformable member 112, and the driving member 114 is used to drive the deformable member 112 to deform.

[0035] It is understandable that the deformable member 112 can be closed by controlling the channel 113 through its own deformation. The liquid can squeeze the deformable member 112 under the action of the driving force, so that a thin slit channel is formed between the deformable member 112 and the side wall 111, and the liquid passes through the thin slit channel to form jet droplets. In other words, the deformable member 112 can form a valve that controls the opening and closing of the channel 113 through its own deformation. When the valve is closed, the liquid can break through the valve under the action of a strong driving force and be ejected from the thin slit channel with almost no height in a very thin posture, thereby forming jet droplets. The liquid can form stable jet droplets of uniform size through the thin slit channel, and can also ensure a high flux of droplets.

[0036] Optionally, in some embodiments, the deformable part 112 includes an elastic deformable membrane, two sides of which are respectively connected to two sides of the side wall 111, so that the elastic deformable membrane and the side wall 111 enclose a channel 113, and when the elastic deformable membrane is deformed, it can bulge toward the side wall 111 to fit with the side wall 111. Figure 2 A schematic structural diagram is shown when the elastically deformable membrane bulges toward the side wall 111 when deformed (the elastically deformable membrane is not in contact with the side wall 111 ).

[0037] It can be understood that when the elastically deformable membrane bulges toward the side wall 111 to fit with the side wall 111 , the elastically deformable membrane can close the channel 113 .

[0038] Of course, the deformable member 112 may also include other deformable structures that can control the opening and closing of the channel 113 by its own deformation, and no specific limitation is made here. For example, the deformable member 112 may also include an airbag, and the size of the airbag is controlled by inflating the airbag, thereby opening and closing the channel 113, which will not be described in detail here.

[0039] Optionally, in some embodiments, the driving member 114 includes a pressurized air chamber, one side of the elastically deformable membrane is located in the pressurized air chamber, and the elastically deformable membrane is driven to deform when the pressurized air chamber is pressurized. Optionally, the side of the elastically deformable membrane facing away from the channel 113 is located in the pressurized air chamber.

[0040] Optionally, the elastic deformable membrane may be continuously loaded with air pressure through a pressurized air chamber to drive the elastic deformable membrane to deform. Optionally, the air pressure may be set to a constant pressure or a regularly high-frequency changing air pressure.

[0041] Exemplarily, when the air pressure is set to a regularly high-frequency changing air pressure, the pressurized air chamber can alternately apply low-frequency air pressure and high-frequency air pressure to the elastically deformable membrane, thereby achieving vibration of the elastically deformable membrane, promoting the breakup of the jet and the uniform generation of droplets.

[0042] It is understandable that the degree of closure of the channel can be controlled by adjusting the pressurization method and the pressurization size of the pressurized air chamber to control the degree of extrusion between the elastic deformation membrane and the side wall 111. The greater the extrusion force between the elastic deformation membrane and the side wall 111, the higher the degree of closure of the channel, and the greater the difficulty for the liquid to break through the valve. When the extrusion force between the elastic deformation membrane and the side wall 111 is large enough, the elastic deformation membrane can cut off the continuous fluid.

[0043] For example, when the pressurized air chamber applies low-frequency air pressure to the elastically deformable membrane, the liquid can break through the valve to eject; when the pressurized air chamber applies high-frequency air pressure to the elastically deformable membrane, the elastically deformable membrane can cut off the continuous fluid to form droplets. By continuously and alternately applying low-frequency air pressure and high-frequency air pressure to the elastically deformable membrane, the generation of droplets can be promoted.

[0044] Optionally, in some embodiments, the elastically deformable membrane is made of a flexible material.

[0045] Optionally, in some embodiments, the elastic deformation membrane is a thin film. For example, the thin film can be made of a flexible material such as PDMS or silica gel, which is not specifically limited here.

[0046] It should be noted that, compared with conventional step-type droplet generation, the elastic deformation membrane in the embodiment of the present application adopts a thin film structure, which can generate film vibration under the drive of the pressurized air chamber, which can promote the uniformity of droplet generation and the breakage of jet droplets. In addition, the embodiment of the present application can realize a height-free step-type droplet generation structure by using film deformation.

[0047] Optionally, the top of the side wall 111 is arc-shaped. Arranging the top of the side wall 111 into an arc shape is conducive to the elastic deformation membrane being in contact with the side wall 111 when it bulges.

[0048] Exemplarily, the side wall 111 is a first curved side wall, and the film is a flat film. When the film bulges toward one side of the side wall 111, it can almost fit with the first curved side wall, so that a thin slit channel can be formed between the film and the first curved side wall for liquid to pass through and form stable jet droplets of uniform size.

[0049] Optionally, the cross section of the side wall 111 is semicircular. Of course, the cross section of the side wall 111 may also be semi-elliptical or other arc shapes, which is not specifically limited here.

[0050] Other structures and operations of the droplet generation structure 110 according to the embodiment of the present application are known to ordinary technicians in the field and will not be described in detail here.

[0051] See also Figures 3 to 5 The embodiment of the present application also provides a microfluidic channel, which includes the above-mentioned droplet generation structure 110, and also includes a channel body 120 having an inlet 1211 and an outlet, the inlet 1211 is connected to the outlet, and the channel 113 is connected to one end of the outlet of the channel body 120.

[0052] Alternatively, see Figure 6 The flow channel body 120 includes a vertical portion 121 and a horizontal portion 122 connected to each other. The end of the vertical portion 121 away from the horizontal portion 122 is an inlet 1211, and the end of the horizontal portion 122 away from the vertical portion 121 is an outlet. Optionally, the inlet 1211 can be used to inject water into the flow channel body 120.

[0053] Alternatively, see Figure 6 and Figure 7 The horizontal portion 122 may include a plane side wall 1221 and a second arc-shaped side wall 1222. The two sides of the plane side wall 1221 are respectively connected to the two sides of the second arc-shaped side wall 1222 to enclose a horizontal flow channel 1223 for liquid to pass through. The outlet end of the plane side wall 1221 is connected to one end of the film, and the outlet end of the second arc-shaped side wall 1222 is connected to one end of the first arc-shaped side wall.

[0054] Optionally, the cross section of the second arc-shaped side wall 1222 is a semicircle of the same size as the cross section of the first arc-shaped side wall. Of course, the cross section of the second arc-shaped side wall 1222 can also be a semi-ellipse or other arc shape, which is not specifically limited here.

[0055] Optionally, in some embodiments, the flow channel body 120 is integrally formed with the side wall 111. Exemplarily, the first arcuate side wall is formed by extending the second arcuate side wall 1222 along the flow direction of the liquid.

[0056] Optionally, the flow channel body 120 and the first arc-shaped side wall are made of one or more materials such as DMS, silica gel, COC, COP, PS, PC, etc., which are not specifically limited here. It should be noted that DMS, silica gel, COC, COP, PS, PC, etc. are hard or soft materials in the field of microfluidics.

[0057] Optionally, in some embodiments, see Figure 3The microfluidic channel further includes a droplet collecting mechanism 130 , which is connected to an end of the droplet generating structure 110 away from the channel body 120 .

[0058] Optionally, in some embodiments, the liquid filled in the droplet collection mechanism 130 is incompatible with the droplets to achieve the effect of droplet collection. Optionally, when generating water phase droplets, the droplet collection mechanism 130 can be a droplet collection box filled with oil phase. Of course, the droplet collection mechanism 130 can also be other droplet collection structures that can achieve droplet collection, and no specific limitation is made here.

[0059] Optionally, the width of the oil phase channel 131 of the droplet collection mechanism 130 is greater than the width of the channel 113 .

[0060] Exemplarily, one end of the film away from the planar side wall 1221 is connected to the bottom wall of the oil phase channel 131, and one end of the first curved side wall away from the second curved side wall 1222 is connected to the side wall 111 of the oil phase channel 131, so that one end of the droplet generating structure 110 away from the flow channel body 120 is connected to the oil phase channel 131.

[0061] Other structures and operations of the microfluidic channel according to the embodiments of the present application are known to those skilled in the art and will not be described in detail here.

[0062] See also Figure 3 and Figure 6 The embodiment of the present application also provides a microfluidic chip, which includes the above-mentioned microfluidic channel, and also includes a first plate body 210 and a second plate body 220. The first plate body 210 is provided with a first groove, and the second plate body 220 is provided with a second groove. The driving member is arranged in the second groove, and the deformable member is connected to the outer periphery of the opening of the second groove. The first plate body 210 and the second plate body 220 are covered and connected so that the first groove and the second plate body 220 are enclosed to form a channel body, and the first groove and the deformable member are enclosed to form a channel.

[0063] Optionally, the second slot is a pressurized air chamber.

[0064] Optionally, a third groove communicating with the first groove is further formed on the first plate body 210 . When the first plate body 210 and the second plate body 220 are covered, the third groove and the second plate body 220 are enclosed to form an oil phase channel 131 .

[0065] Optionally, the first plate body 210 and the second plate body 220 may be made of PDMS material.

[0066] It is understandable that the first groove and the second groove are processed on the first plate 210, the pressurized air chamber is processed on the second plate 220, and a driving member (elastic deformation membrane) is arranged at the opening of the pressurized air chamber, and the first plate 210 and the second plate 220 are covered and connected to obtain a microfluidic chip with a microfluidic flow channel. This structural setting is conducive to the processing of microfluidic chips.

[0067] Other structures and operations of the microfluidic chip according to the embodiment of the present application are known to ordinary technicians in the field and will not be described in detail here.

[0068] An embodiment of the present application also provides a droplet generation method. Exemplarily, the droplet generation method includes the following steps.

[0069] Step S100 : injecting liquid into the flow channel body 120 through the inlet 1211 of the flow channel body 120 .

[0070] Optionally, in some embodiments, the step S100 may include the following steps: injecting a first liquid into the flow channel body 120 through the inlet 1211 of the flow channel body 120 , and filling the droplet collection mechanism 130 with a second liquid that is incompatible with the first liquid.

[0071] Optionally, the first liquid is an aqueous phase liquid, and the second liquid is an oil phase liquid.

[0072] Optionally, liquid can be injected into the flow channel body 120 through an injection member. Optionally, the injection member can be a micro pump. Figure 4 The injection member (not shown in the drawings) can provide a driving force to drive the first fluid in the flow channel body 120 to flow to the right, and can also prevent the second liquid in the droplet collection mechanism 130 from flowing into the flow channel body 120.

[0073] Step S200, the deformable member 112 is driven to deform by the driving member 114 to close the channel 113. The liquid in the flow channel body 120 squeezes the deformable member 112 under the action of the driving force to form a thin slit channel between the deformable member 112 and the side wall 111. The liquid passes through the thin slit channel to form jet droplets and is ejected.

[0074] Optionally, in some embodiments, the above step S200 may include the following steps: the deformable member 112 is driven to deform by the driving member 114 to close the channel 113, and the liquid in the flow channel body 120 squeezes the deformable member 112 under the action of the driving force to form a thin slit channel between the deformable member 112 and the side wall 111, and the liquid in the flow channel body 120 passes through the thin slit channel to form jet droplets, and the jet droplets are sprayed into the second liquid in the droplet collection mechanism 130 to collect the generated droplets.

[0075] Optionally, in some embodiments, the driving member 114 includes a pressurized air chamber, and the deformable member 112 includes an elastic deformable membrane. The elastic deformable membrane is continuously loaded with air pressure through the pressurized air chamber to drive the elastic deformable membrane to deform. The air pressure of the pressurized air chamber is set to a constant pressure or a regularly high-frequency changing air pressure.

[0076] It is understandable that the degree of closure of the channel can be controlled by adjusting the pressurization method and the pressurization size of the pressurized air chamber to control the degree of extrusion between the elastic deformation membrane and the side wall 111. The greater the extrusion force between the elastic deformation membrane and the side wall 111, the higher the degree of closure of the channel, and the greater the difficulty for the liquid to break through the valve. When the extrusion force between the elastic deformation membrane and the side wall 111 is large enough, the elastic deformation membrane can cut off the continuous fluid.

[0077] For example, when the pressurized air chamber applies low-frequency air pressure to the elastically deformable membrane, the liquid can break through the valve to eject; when the pressurized air chamber applies high-frequency air pressure to the elastically deformable membrane, the elastically deformable membrane can cut off the continuous fluid to form droplets. By continuously and alternately applying low-frequency air pressure and high-frequency air pressure to the elastically deformable membrane, the generation of droplets can be promoted.

[0078] Optionally, in some embodiments, the elastically deformable membrane is made of a flexible material.

[0079] Optionally, in some embodiments, the elastic deformation membrane is a thin film. For example, the thin film can be made of a flexible material such as PDMS or silica gel, which is not specifically limited here.

[0080] Optionally, the droplet collection mechanism 130 is a droplet collection box filled with oil phase. The water phase liquid in the flow channel body 120 passes through the thin slit channel to form stable jet droplets of uniform size, which are sprayed into the oil phase in the droplet collection box. This process can be called a stable jet droplet generation mode.

[0081] The other steps and operations of the droplet generation method according to the embodiment of the present application are known to ordinary technicians in the field and will not be described in detail here.

[0082] In the description of this specification, if the reference terms "one embodiment", "some instances", "some embodiments", "illustrative embodiment", "example", "specific example", or "some examples" appear, it means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in the embodiments or examples of this application. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0083] The above describes the implementation methods of the present application in detail in conjunction with the accompanying drawings, but the present application is not limited to the above implementation methods. Various changes can be made within the knowledge scope of ordinary technicians in the technical field without departing from the purpose of the present application.

[0084] In the description of this application, if "," appears in the patent name, it means an "and" relationship, not an "or" relationship. For example, if the patent name is "a kind of A, a kind of B", it means that the content protected by this application is: the technical solution with the subject name A and the technical solution with the subject name B.

Claims

1. A droplet generation structure, characterized in that: include: Side wall; A deformable member, the deformable member is connected to the side wall to form a channel, and the deformable member is used to control the opening and closing of the channel by its own deformation; A driving member, wherein the driving member is connected to the deformable member, and the driving member is used to drive the deformable member to deform.

2. The droplet generation structure according to claim 1, characterized in that: The deformable member includes an elastic deformation membrane, two sides of which are respectively connected to two sides of the side wall, so that the elastic deformation membrane and the side wall enclose the channel, and when the elastic deformation membrane is deformed, it can bulge toward the side wall to fit with the side wall.

3. The droplet generation structure according to claim 2, characterized in that: The driving member comprises a pressurized air chamber, one side of the elastic deformation membrane is located in the pressurized air chamber, and the pressurized air chamber drives the elastic deformation membrane to deform when pressurized.

4. The droplet generation structure according to claim 1 or 2, characterized in that: The top of the side wall is arc-shaped.

5. A microfluidic channel, characterized in that: The liquid droplet generation structure according to any one of claims 1 to 4 further comprises a flow channel body having an inlet and an outlet, wherein the channel is connected to one end of the outlet of the flow channel body.

6. The microfluidic channel according to claim 5, characterized in that: The microfluidic channel further comprises a droplet collecting mechanism, which is connected to an end of the droplet generating structure away from the channel body.

7. The microfluidic channel according to claim 6, characterized in that: The liquid filled in the liquid droplet collecting mechanism is incompatible with the liquid droplets.

8. A microfluidic chip, characterized in that: The microfluidic channel comprises the microfluidic channel described in any one of claims 5 to 7, and further comprises a first plate body and a second plate body, the first plate body is provided with a first groove, the second plate body is provided with a second groove, the driving member is arranged in the second groove, the deformable member is connected to the outer periphery of the opening of the second groove, the first plate body and the second plate body are covered and connected so that the first groove and the second plate body enclose the channel body, and the first groove and the deformable member enclose the channel.

9. A method for generating droplets, characterized in that: The steps include: injecting liquid into the flow channel body through the inlet of the flow channel body; The deformable member is driven to deform by a driving member to close the channel. The liquid in the flow channel body squeezes the deformable member under the action of the driving force to form a thin slit channel between the deformable member and the side wall. The liquid passes through the thin slit channel to form jet droplets and is ejected.

10. The droplet generation method according to claim 9, characterized in that: The driving member includes a pressurized air chamber, and the deformable member includes an elastic deformable membrane. The elastic deformable membrane is continuously loaded with air pressure through the pressurized air chamber to drive the elastic deformable membrane to deform. The air pressure of the pressurized air chamber is set to a constant pressure or a regularly high-frequency changing air pressure.

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