Green residue-free skin care detergent and preparation method thereof

Abstract

The invention discloses a green and residue-free skincare detergent. The detergent is prepared from the following components in percentage by weight: (1) 8-12% of cocamidopropyl betaine; (2) 10 to 15 percent of decyl glucoside; (3) 2-5% of glycerol; (4) 1-3% of an aloe extract; 1-2% of a chamomile extract; (6) 0.5 to 1 percent of ethanol; and (7) 65-75% of purified water. The surfactants used in the invention are all natural sources, can be completely biodegraded and are mild to skin. The aloe extract, the chamomile extract and other components are used in the formula, so that the skin care effect is further enhanced. According to the invention, a preparation process combining low-temperature stirring and low-temperature extraction is adopted, so that active ingredients of plants are effectively reserved, and activity loss caused by high temperature is avoided. Therefore, the detergent disclosed by the invention has a good application effect and is suitable for large-scale production and application.

Description

Technical Field

[0001] The invention belongs to the field of daily chemicals and cosmetics, and particularly relates to a green, residue-free, mild skin-protecting detergent and a preparation method thereof. Background Art

[0002] As daily cleaning products, dishwashing liquids are widely used in tableware, kitchen utensils and some skin cleaning. However, most dishwashing liquid products on the market are mainly composed of petrochemical-derived surfactants, synthetic preservatives, fragrances and other chemical ingredients. Although they have strong decontamination capabilities, long-term use may have adverse effects on the skin and the environment. These chemical ingredients are often difficult to rinse completely after use and may leave residues on the surface of tableware or skin. In particular, ingredients such as petrochemical surfactants and phenol preservatives remain on the skin, which can easily lead to adverse reactions such as skin allergies, dryness, and itching. Especially for people with sensitive skin and children, it may cause more serious skin irritation. In addition, chemical surfactants discharged into water bodies after use will also cause water pollution and cause a certain degree of harm to aquatic organisms.

[0003] In recent years, as consumers' awareness of health and environmental protection has increased, the demand for dishwashing liquids has gradually expanded from traditional cleaning functions to mild, residue-free, safe and environmentally friendly aspects. Green, natural and safe daily chemical products are increasingly favored by consumers, and plant extracts and natural surfactants have gradually become an important direction for innovation in dishwashing liquid formulas. For example, plant-derived surfactants such as cocamidopropyl betaine and sugar-based surfactants have attracted much attention for their mild, low-irritation and biodegradability. Plant surfactants are not only mild to the skin, but also have good cleaning power. They can be quickly decomposed by microorganisms in the ecological environment, reducing the risk of pollution to water and soil. In addition, the application of plant extracts (such as aloe vera, chamomile, etc.) in skin care products has proven that they have skin care effects such as moisturizing, anti-inflammatory and repair. Applying these ingredients to dishwashing liquids can not only achieve a cleaning effect, but also play a gentle care role on the skin.

[0004] At present, although some dishwashing liquids claiming to have "natural ingredients" have appeared on the market, many product formulas still have problems such as high proportion of chemical ingredients, unstable plant ingredients, and single efficacy, and fail to achieve the characteristics of true green, safe, and no residue. Therefore, developing a dishwashing liquid with natural ingredients, no residue, and skin care effects has become an urgent problem to be solved in the industry. This not only conforms to the trend of green environmental protection, but also meets consumers' demand for mild and safe daily chemical products. Summary of the invention

[0005] The purpose of the present invention is to provide a green, residue-free skin care detergent and a preparation method thereof. The present invention uses natural surfactants, plant extracts and biodegradable ingredients through formula innovation, so that the detergent has the advantages of high-efficiency cleaning, mild skin care and zero residue. At the same time, the preparation method of the present invention optimizes the production process to ensure that the product is green and pollution-free.

[0006] Therefore, the present invention discloses a green, residue-free skin care detergent, the formula of which is:

[0007] (1) Cocamidopropyl betaine: 8-12%;

[0008] (2) Decyl glucoside: 10-15%;

[0009] (3) Glycerol: 2-5%;

[0010] (4) Aloe extract: 1-3%;

[0011] (5) Chamomile extract: 1-2%;

[0012] (6) Ethanol: 0.5-1%;

[0013] (7) Purified water: 65-75%.

[0014] In another aspect, the present invention further discloses a green, residue-free skin-care detergent, the formula of which is:

[0015] (1) Cocamidopropyl betaine: 10%;

[0016] (2) Decyl glucoside: 12%;

[0017] (3) Glycerol: 4%;

[0018] (4) Aloe vera extract: 2%;

[0019] (5) Chamomile extract: 1.5%;

[0020] (6) Ethanol: 0.8%;

[0021] (7) Purified water: 69.7%.

[0022] In another aspect, the present invention further discloses a green, residue-free skin-care detergent, the formula of which is:

[0023] (1) Cocamidopropyl betaine: 10%;

[0024] (2) Decyl glucoside: 12%;

[0025] (3) Glycerol: 4%;

[0026] (4) Aloe vera extract: 2%;

[0027] (5) Chamomile extract: 1.5%;

[0028] (6) Ethanol: 0.8%;

[0029] (7) Purified water: 69.7%;

[0030] (8) Anti-triglyceride monoclonal antibody: 20 mg / L.

[0031] Preferably, the amino acid sequences of the heavy chain variable region and the light chain variable region of the anti-triglyceride monoclonal antibody of the present invention are as shown in SEQ ID NO.1 and SEQ ID NO.2.

[0032] In another aspect, the present invention further discloses a method for preparing the detergent, the method comprising the following steps:

[0033] (1) Raw material pretreatment: The purified water was allowed to stand at 20°C for 48 hours and filtered using a 0.45 μm filter membrane to ensure the cleanliness and sterility of the purified water;

[0034] (2) Surfactant dissolution: Cocamidopropyl betaine and decyl glucoside were added to the pretreated purified water at room temperature in sequence; the stirring speed was controlled at 200 rpm for 20 minutes to prevent excessive stirring from causing decomposition of the components;

[0035] (3) Adding skin care ingredients: Add glycerin to the stirred solution and stir for 10 minutes; then add aloe vera extract and chamomile extract in sequence, stirring for 15 minutes after each ingredient is added to ensure that the ingredients are evenly distributed;

[0036] (4) Adding preservatives: adding ethanol and anti-triglyceride monoclonal antibodies and stirring for 5 minutes; the ethanol concentration is strictly controlled at 0.8% to balance the preservative effect and mildness, and the pH value is adjusted to 6.5-7.5 to ensure the stability of the anti-triglyceride monoclonal antibodies;

[0037] (5) Degassing and filtration: The solution was degassed for 45 minutes and finally filtered using a 0.22 μm microporous filter membrane to ensure that the product was free of bubbles and particles and maintained clarity;

[0038] (6) Filling: After filtration, fill the solution into sterile bottles, seal and store.

[0039] In another aspect, the present invention also discloses a use of the anti-triglyceride monoclonal antibody in the preparation of detergent.

[0040] The surfactants used in the present invention are all of natural origin, completely biodegradable and mild to the skin. Ingredients such as aloe extract and chamomile extract are used in this formula to further enhance the skin care effect. The present invention adopts a preparation process combining low-temperature stirring with low-temperature extraction, effectively retains plant active ingredients, and avoids the activity loss caused by high temperature. Therefore, the detergent of the present invention has a good application effect, and provides a good basis for the large-scale application of the detergent. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 SDS-PAGE detection results of anti-triglyceride monoclonal antibody. DETAILED DESCRIPTION

[0042] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0043] Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art. Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0044] Example 1: Formula of green, residue-free skin care detergent

[0045] 1. The formula of the present invention is composed of surfactants and natural skin care ingredients from various plant sources to ensure its cleaning effect, skin care effect and no residue. The present invention preferably uses the following formula combination:

[0046] (1) Cocamidopropyl Betaine: 8-12%. As a natural surfactant, cocamidopropyl betaine has good detergency, foam stability and biodegradability, is mild to the skin and does not cause skin irritation.

[0047] (2) Decyl glucoside: 10-15%. Sugar-based surfactants are extracted from plants, are easily biodegradable, have mild cleaning properties, and are friendly to the skin and the ecological environment.

[0048] (3) Glycerin: 2-5%. Glycerin, as a moisturizer, can prevent skin moisture loss during cleansing, keep the skin moist and soft, and reduce dryness.

[0049] (4) Aloe vera extract: 1-3%. Aloe vera is rich in polysaccharides, vitamins and amino acids, and has moisturizing, antioxidant and anti-inflammatory effects, which can help soothe skin irritation during cleansing.

[0050] The preparation of aloe vera extract is briefly described as follows: collect fresh, undamaged aloe vera leaves, cut off the thorny parts on the edges, and wash them thoroughly with purified water to ensure that there are no pollutants on the surface of the leaves. Peel the cleaned aloe vera leaves to expose the transparent gel part of the inner layer. Use a sterile knife to separate the gel, avoid contact with the outer skin, so as not to bring in impurities or irritating ingredients. Put the aloe vera gel into a grinding device (such as a blender), add an appropriate amount of purified water, and mix in a ratio of 1:1 (gel: water); stir at a low speed for 3-5 minutes until a uniform aloe vera gel liquid is formed. Use filter cloth or filter paper to filter the aloe vera gel liquid to remove undissolved solid particles. Concentrate the filtrate to a suitable concentration (generally 5 times the concentration), then transfer the extract to a sterile container, seal and store at 4°C in a refrigerated state, avoiding direct sunlight and high temperature.

[0051] (5) Chamomile extract: 1-2%. Chamomile contains natural antioxidants and flavonoids, which can inhibit inflammation and soothe sensitive skin.

[0052] The preparation of chamomile extract is briefly described as follows: select high-quality chamomile flowers, wash them thoroughly and dry them in a cool and dry place, or use dried chamomile flowers directly. Take an appropriate amount of dried chamomile flowers, place the chamomile flowers in a 70% ethanol solution at a ratio of 1:10 (flower material: solvent), seal them and let them stand at room temperature for 24 hours so that the solvent can be fully soaked. The soaked solution is placed in an ultrasonic extraction device, the temperature is set to 40°C, and the extraction time is 30 minutes. The ultrasonic frequency is controlled at 40kHz to effectively extract the active ingredients in chamomile. Filter the extract through filter cloth or filter paper to remove flower residues and obtain a preliminary chamomile extract. Put the filtered chamomile extract into a vacuum evaporator, control the temperature not to exceed 50°C, and evaporate the solvent to the required concentration (generally retain 1 / 5 of the volume). Transfer the concentrated chamomile extract to a sterile container, seal it and store it in a refrigerated environment at 4°C to avoid light and high temperature.

[0053] (6) Ethanol: 0.5-1%. To prevent product contamination, a small amount of ethanol can be added as a preservative, which can prevent the growth of microorganisms without affecting the environmental friendliness of the product.

[0054] (7) Purified water: 65-75%. As a matrix component, it is used to dissolve various components and provide appropriate viscosity.

[0055] 2. Based on the above formula, the precise proportion of the preferred detergent formula 1 of the present invention is as follows:

[0056] (1) Cocamidopropyl betaine: 10%;

[0057] (2) Decyl glucoside: 12%;

[0058] (3) Glycerol: 4%;

[0059] (4) Aloe vera extract: 2%;

[0060] (5) Chamomile extract: 1.5%;

[0061] (6) Ethanol: 0.8%;

[0062] (7) Purified water: 69.7%.

[0063] 3. Based on the above formula, the precise proportion of the preferred detergent formula 2 of the present invention is as follows:

[0064] (1) Cocamidopropyl betaine: 10%;

[0065] (2) Decyl glucoside: 12%;

[0066] (3) Glycerol: 4%;

[0067] (4) Aloe vera extract: 2%;

[0068] (5) Chamomile extract: 1.5%;

[0069] (6) Ethanol: 0.8%;

[0070] (7) Purified water: 69.7%;

[0071] (8) Anti-triglyceride monoclonal antibody: 20 mg / L.

[0072] Example 2: Preparation and testing of anti-triglyceride monoclonal antibodies

[0073] 1. Preparation of anti-triglyceride monoclonal antibodies

[0074] (1) Conjugate triglycerides with BSA. Dissolve triglyceride-BSA antigen in FCA, prepare an emulsion and inject it into the mouse peritoneum (0.2 ml, concentration 200 μg / mouse). Mix triglyceride-BSA antigen with IFA and inject it into the peritoneum every two weeks, 0.1 ml each time, for 3-4 consecutive immunizations. One week after immunization, detect the anti-triglyceride antibody titer in mouse serum by ELISA. Those with a titer of 1:10,000 or above can be used for fusion.

[0075] (2) Remove the spleen of high-titer mice, separate spleen cells and count them. Mix spleen cells with SP2 / 0 myeloma cells in a ratio of 5:1, and add PEG to induce cell fusion. Then gradually add RPMI 1640 medium to wash and terminate the fusion reaction. Place the fused cells in a 96-well plate, 100 μL per well, and selectively culture them using HAT medium at a culture temperature of 37°C and 5% CO2. Replace the HAT medium every 2-3 days.

[0076] (3) 10-14 days after fusion, detect anti-triglyceride antibodies in the supernatant by ELISA and select positive wells. Subclone the selected positive hybridoma cells to ensure monoclonal specificity. The limiting dilution method is usually used for 3-4 rounds of screening until a stable clone is obtained. The selected clone is expanded and cultured. In this study, a better hybridoma cell was screened and named hybridoma cell 1D4.

[0077] (4) Hybridoma cells 1D4 were cultured on a large scale. Each mouse was intraperitoneally injected with 2×10 7 Up to 1×10 8 hybridoma cells. Monitor the health of the mice weekly after injection. Begin collecting ascites 1-2 weeks after injection, and collect when the volume reaches 10-20 ml. Use a sterile syringe to collect ascites from the mouse peritoneal cavity and immediately transfer to a refrigerated storage environment. After collecting the ascites, filter it to remove cells and impurities.

[0078] (5) Prepare the ProteinA / G affinity column. Pass the filtered ascites sample through the affinity chromatography column, using PBS buffer to slowly pass at a flow rate of 0.5 ml / min to ensure that the antibody binds to the affinity column. Wash the affinity column with PBS to remove unbound impurities. Elute the antibody with a low-salt eluent (glycine buffer, pH 2.7-3.0). Collect the eluate, collecting 0.5 ml each time. Neutralize the eluate with 1 M Tris-HCl buffer (pH 8.0) to restore the stability of the antibody.

[0079] (6) The purified antibody solution is dialyzed through a dialysis bag to remove low molecular weight impurities and solvents. After dialysis, the solution is sterilized by filtering through a 220 nm filter membrane, and then quantitatively divided and stored at -80°C.

[0080] 2. Testing of anti-triglyceride monoclonal antibodies

[0081] (1) Concentration detection: The purified antibody was detected using a BCA kit, and its concentration was 6.8 mg / ml, which translates to an ascites expression yield of approximately 10 mg / ml.

[0082] (2) Purity test: Take an appropriate amount of purified monoclonal antibody and perform SDS-PAGE test. The results show that ( Figure 1 ), its purity can reach more than 95%.

[0083] (3) ELISA antibody titer detection: Coat the triglyceride-BSA antigen in the ELISA plate, add the sample antibody (pre-diluted to 1 mg / ml and then further diluted) for incubation, and then add the enzyme-labeled secondary antibody and TMB colorimetric reagent. Measure the OD value at 450nm. The results showed that when the antibody titer was 1:128000, the OD450 value was 1.2. This shows that the monoclonal antibody has good sensitivity.

[0084] (4) Sequence determination: Total RNA was extracted from hybridoma cell 1D4. TRIzol or RNA extraction kit was used to obtain high-quality RNA according to the operating procedures. The extracted RNA was used as a template and reverse transcriptase (such as M-MLV or SuperScript III) was used for reverse transcription to generate cDNA. Specific primers were designed according to the light chain and heavy chain of the antibody, and PCR amplification was performed using cDNA as a template. The antibody variable region fragment obtained by PCR amplification was purified, connected to pMD18-T, and transformed into Escherichia coli DH5α for cloning. Positive clones were selected and plasmid DNA was extracted. It was sent to a sequencing company for bidirectional sequencing. After analysis, the amino acid sequences of the heavy chain variable region and the light chain variable region of the monoclonal antibody are shown in SEQ ID NO.1 and SEQ ID NO.2.

[0085] 3. Preparation and expression of anti-triglyceride monoclonal antibodies in CHO cells

[0086] (1) Based on the determined antibody light chain and heavy chain variable region sequences, design the gene sequences of the full-length light chain and heavy chain. Connect the gene sequences of the light chain and heavy chain to the IgG constant region, and obtain the complete antibody gene sequence through DNA synthesis. Clone the heavy chain and light chain genes into the CHO cell expression vector (pcDNA3.1) respectively. Confirm the absence of missense mutations or insertion mutations by sequencing. Then use Lipofectamine 3000 to co-transfect the heavy chain and light chain vectors into CHO cells.

[0087] (2) The transfected CHO cells are placed in a medium containing G418 to screen out stably transfected cell lines. The screened CHO cells are further cloned using limiting dilution or flow cytometry to select monoclonal cell lines that highly express antibodies. The antibody content in the culture supernatant of different clones is analyzed using ELISA or Western Blot, and high-expressing clones are selected for large-scale culture.

[0088] (3) The selected high-expressing CHO cell lines were inoculated into serum-free culture medium, and the culture system was gradually expanded to a large-capacity bioreactor (5 L). The culture supernatant was collected and centrifuged every 48-72 hours to remove cells and impurities, and the supernatant with a higher antibody content was collected.

[0089] (4) Purify the antibody using a Protein A or Protein G affinity chromatography column. Load the collected supernatant onto the affinity column and wash with a buffer solution (PBS) after binding to remove nonspecific proteins. Elute the antibody from the affinity column using an acidic elution buffer (citrate buffer at pH 3.0), and immediately neutralize the eluate to protect the antibody structure. Replace the buffer solution (PBS) by dialysis or ultrafiltration, sterilize, and store at -20°C for future use.

[0090] (5) After the above steps, this study screened a better CHO cell strain, whose fermentation culture expressed anti-triglyceride monoclonal antibody production could reach 15 g / L. And the specificity and sensitivity tests were consistent with the monoclonal antibody prepared from mouse ascites. Therefore, the preparation method of the anti-triglyceride monoclonal antibody of the present invention is simple and low in cost, meeting the needs of large-scale application.

[0091] Example 3: Preparation of green, residue-free skin care detergent

[0092] (1) Raw material pretreatment: The purified water was allowed to stand at 20°C for 48 hours and filtered using a 0.45 μm filter membrane to ensure the cleanliness and sterility of the purified water;

[0093] (2) Surfactant dissolution: Cocamidopropyl betaine and decyl glucoside were added to the pretreated purified water at room temperature (25°C) in sequence; the stirring speed was controlled at 200 rpm for 20 minutes to prevent excessive stirring from causing decomposition of the components;

[0094] (3) Adding skin care ingredients: Add glycerin to the stirred solution and stir for 10 minutes; then add aloe vera extract and chamomile extract in sequence, stirring for 15 minutes after each ingredient is added to ensure that the ingredients are evenly distributed;

[0095] (4) Adding preservatives: Add ethanol and anti-triglyceride monoclonal antibodies (required for Formulation 2, not required for Formulation 1), and stir for 5 minutes; the ethanol concentration is strictly controlled at 0.8% to balance the preservative effect and mildness, and the pH value is adjusted to 6.5-7.5 to ensure the stability of the anti-triglyceride monoclonal antibodies;

[0096] (5) Degassing and filtration: The solution was degassed for 45 minutes and finally filtered using a 0.2 μm microporous filter membrane to ensure that the product was free of bubbles and particles and maintained clarity;

[0097] (6) Filling: After filtration, fill the solution into sterile bottles, seal and store.

[0098] Example 4: Application of green, residue-free skin care detergent

[0099] 1. Cleaning effect test

[0100] An equal amount of oil stains were applied to a standard ceramic sheet, and then the two detergents of the present invention (prepared with formula 1 and formula 2, detergent 1 and detergent 2, respectively) and a commercial detergent (Liby natural fruit vinegar concentrated detergent) were used for cleaning. The same dilution ratio (1:20) was used, and each test was repeated 5 times. The amount of oil stains remaining on the ceramic sheet after cleaning (mg) was recorded, and the average decontamination rate was calculated.

[0101] The results show (Table 1) that the average decontamination rates of the detergents 1 and 2 of the present invention are both above 92.8%, both higher than 89.9% of the commercially available detergents. The detergent 2 is better than the detergent 1, which indicates that the anti-triglyceride monoclonal antibody added in the present invention can further promote cleaning and degreasing, and has a good application effect.

[0102] Table 1 Cleaning effect test results

[0103]

[0104] 2. Skin irritation test

[0105] A skin smearing experiment was conducted on 20 volunteers. The two detergents of the present invention (prepared by formula 1 and formula 2, detergent 1 and detergent 2, respectively) and a commercial detergent (Liby natural fruit vinegar concentrated detergent) were diluted to a concentration of 1:20, applied on the back of the hand, left for 2 minutes and then rinsed, and the reaction scores of each volunteer's skin redness, swelling, itching, etc. (0-5 points, 0 points for no irritation, 5 points for severe irritation) were recorded.

[0106] The results show (Table 2) that the average skin irritation scores of the two dishwashing liquids of the present invention are both 0.4, which is much lower than 2.1 of the commercially available dishwashing liquids, indicating that the two dishwashing liquids of the present invention are more gentle to the skin.

[0107] Table 2 Skin irritation test results

[0108] Type of detergent Number of testers Average stimulation rating Commercial dishwashing liquid 20 2.1 The detergent of the present invention 1 20 0.4 The present invention detergent 2 20 0.4

[0109] 3. Biodegradability test

[0110] A 28-day biodegradability experiment was conducted with reference to the OECD 301F standard to determine the degradation rates of two detergents of the present invention (prepared with Formula 1 and Formula 2, namely, Detergent 1 and Detergent 2) and a commercially available detergent (Liby natural fruit vinegar concentrated detergent) in a microbial environment.

[0111] The results show (Table 3) that the degradation rates of the two detergents of the present invention within 28 days are both about 95%, while the commercial detergents are only 47%. This data verifies that the two detergents of the present invention have high biodegradability in the natural environment and are more friendly to the ecological environment.

[0112] Table 3 Biodegradability test results

[0113]

[0114] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A green, residue-free skin care detergent, characterized in that: The formula of the detergent is: (1) Cocamidopropyl betaine: 8-12%; (2) Decyl glucoside: 10-15%; (3) Glycerol: 2-5%; (4) Aloe extract: 1-3%; (5) Chamomile extract: 1-2%; (6) Ethanol: 0.5-1%; (7) Purified water: 65-75%.

2. A green, residue-free skin care detergent, characterized in that: The formula of the detergent is: (1) Cocamidopropyl betaine: 10%; (2) Decyl glucoside: 12%; (3) Glycerol: 4%; (4) Aloe vera extract: 2%; (5) Chamomile extract: 1.5%; (6) Ethanol: 0.8%; (7) Purified water: 69.7%.

3. A green, residue-free skin care detergent, characterized in that: The formula of the detergent is: (1) Cocamidopropyl betaine: 10%; (2) Decyl glucoside: 12%; (3) Glycerol: 4%; (4) Aloe vera extract: 2%; (5) Chamomile extract: 1.5%; (6) Ethanol: 0.8%; (7) Purified water: 69.7%; (8) Anti-triglyceride monoclonal antibody: 20 mg / L.

4. The detergent according to claim 3, characterized in that: The amino acid sequences of the heavy chain variable region and the light chain variable region of the anti-triglyceride monoclonal antibody are shown in SEQ ID NO.1 and SEQ ID NO.

2.

5. A method for preparing the detergent according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: (1) Raw material pretreatment: The purified water was allowed to stand at 20°C for 48 hours and filtered using a 0.45 μm filter membrane to ensure the cleanliness and sterility of the purified water; (2) Surfactant dissolution: Cocamidopropyl betaine and decyl glucoside were added to the pretreated purified water at room temperature in sequence; the stirring speed was controlled at 200 rpm for 20 minutes to prevent excessive stirring from causing decomposition of the components; (3) Adding skin care ingredients: Add glycerin to the stirred solution and stir for 10 minutes; then add aloe vera extract and chamomile extract in sequence, stirring for 15 minutes after each ingredient is added to ensure that the ingredients are evenly distributed; (4) Adding preservatives: adding ethanol and anti-triglyceride monoclonal antibodies and stirring for 5 minutes; the ethanol concentration is strictly controlled at 0.8% to balance the preservative effect and mildness, and the pH value is adjusted to 6.5-7.5 to ensure the stability of the anti-triglyceride monoclonal antibodies; (5) Degassing and filtration: The solution was degassed for 45 minutes and finally filtered using a 0.22 μm microporous filter membrane to ensure that the product was free of bubbles and particulate matter and maintained clarity; (6) Filling: After filtration, fill the solution into sterile bottles, seal and store.

6. Use of the anti-triglyceride monoclonal antibody according to claim 4 in the preparation of detergent.

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