Plorizin glycosylation gene and application thereof

Through gene mining, it was found that the PGGT1.1 and PGGT1.2 genes can catalyze the glycosylation of root sacin 2’-O-xylose glucoside to form root sacin 2’-O-xylose glucoside, which solved the difficulties encountered in the separation and extraction process in the prior art, and achieved a significant increase in the content of root sacin 2’-O-xylose glucoside and simplified isolation and extraction.

CN119639725AActive Publication Date: 2025-03-18NORTHWEST A & F UNIV
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Patent Information

Application Number
CN202411912555.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-24
Publication Date
2025-03-18
Estimated Expiration
2044-12-24

AI Technical Summary

Technical Problem

The prior art is difficult to efficiently catalyze root sacin glycosylation to form root sacin 2’-O-xylose glucoside, especially in the process of separation and extraction in fruits.

Method used

Through gene mining, it was found that the PGGT1.1 and PGGT1.2 genes can catalyze the glycosylation of root cerin 2’-O-xylose glucoside to form root cerin 2’-O-xylose glucoside, and improve catalytic efficiency by regulating the expression or enzyme activity of these genes.

Benefits of technology

The content of root 2’-O-xylose glucoside was significantly improved, especially in apple leaves, and its separation and extraction process was simplified and it has important application value.

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Abstract

The invention belongs to the technical field of plant genetic engineering, and particularly discloses phlorizin glycosylation protease, a gene and application of the phlorizin glycosylation protease. According to the application, glycosyl transferase genes PGGT1.1 and PGGT1.2 for synthesizing phloretin 2 '-O-xylose glucoside through phlorizin glycosylation are obtained, the PGGT1.1 and the PGGT1.2 are over-expressed in plants, and the content of phloretin 2'-O-xylose glucoside in plant leaves is obviously increased, so that biosynthesis, separation and extraction of phloretin 2 '-O-xylose glucoside are facilitated.
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