Sample analysis method and sample analyzer

Abstract

The invention discloses a sample analysis method and a sample analyzer. The sample analysis method comprises the following steps: preparing a first sample solution and a second sample solution; detecting the first sample liquid through the optical channel to obtain a first detection result, and detecting the second sample liquid through the impedance channel to obtain a second detection result; judging the signal quality of the optical channel and the impedance channel based on the first detection result and the second detection result; and calculating parameter results of red blood cells and blood platelets according to the signal quality. Compared with the prior art, the optical channel reference dimension is added, the parameter results of the red blood cells and the blood platelets are calculated by combining the detection results of the optical channel and the impedance channel, and the problem that the measurement accuracy of the red blood cells and the blood platelets is reduced when errors occur when abnormity is judged by independently adopting the impedance channel is avoided.

Description

Technical Field

[0001] The present application relates to the field of blood cell analysis, and in particular to a sample analysis method and a sample analyzer. Background Art

[0002] Blood cell analysis is an analytical testing technology widely used in clinical testing, which mainly includes the measurement of red blood cells and platelets. Accurate measurement of red blood cells and platelets is of great significance for the diagnosis and detection of diseases.

[0003] At present, when the impedance method and the optical method are combined to measure red blood cells and platelets, the method used is to start the optical method for measurement only when an abnormality is detected by the impedance method. However, when an error occurs when the impedance method is used alone to determine an abnormality, this method will result in not starting the optical method detection and directly using the abnormal results of the impedance method to calculate the parameter results of red blood cells and platelets; or mistakenly entering the optical method detection and selecting the optical detection results with poor detection accuracy to calculate the parameter results of red blood cells and platelets, which will lead to inaccurate red blood cell and platelet measurement results. Summary of the invention

[0004] The main technical problem solved by the present application is to improve the accuracy of red blood cell and platelet measurement, and to provide a sample analysis method and a sample analyzer that can improve the accuracy of red blood cell and platelet measurement.

[0005] In order to solve the above technical problems, a technical solution adopted in the present application is: providing a sample analysis method, the method comprising: preparing a first sample liquid and a second sample liquid; detecting the first sample liquid through an optical channel to obtain a first detection result, and detecting the second sample liquid through an impedance channel to obtain a second detection result; judging the signal quality of the optical channel and the impedance channel based on the first detection result and the second detection result; and calculating the parameter results of red blood cells and platelets according to the signal quality.

[0006] In order to solve the above technical problems, another technical solution adopted by the present application is: to provide a sample analyzer, including: an optical channel, an impedance channel, and a controller; wherein the controller is connected to the optical channel and the impedance channel respectively; the optical channel is used to detect a first test result of red blood cells and platelets; the impedance channel is used to detect a second test result of red blood cells and platelets; the controller is used to determine the signal quality of the optical channel and the impedance channel, and calculate the parameter results of the red blood cells and platelets according to the signal quality.

[0007] The beneficial effects of the present application are: preparing a first sample liquid and a second sample liquid; detecting the first sample liquid through an optical channel to obtain a first detection result, and detecting the second sample liquid through an impedance channel to obtain a second detection result; judging the signal quality of the optical channel and the impedance channel based on the first detection result and the second detection result; and calculating the parameter results of red blood cells and platelets according to the signal quality. Compared with the prior art, the impedance channel and the optical channel of the present application do not perform another detection method based on the result of a certain detection method, but calculate the parameter results of red blood cells and platelets in combination with the detection results of the optical channel and the impedance channel, thereby avoiding the problem of a decrease in the measurement accuracy of red blood cells and platelets when an error occurs when using the impedance channel alone to judge an abnormality. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative work, among which:

[0009] Figure 1 is a flow chart of an exemplary embodiment of a sample analysis method shown in the present application;

[0010] Figure 2 yes Figure 1 A schematic flow chart of an exemplary embodiment of step S140 in the sample analysis method is shown;

[0011] Figure 3 yes Figure 2 A schematic flow chart of an exemplary embodiment of step S210 in the sample analysis method is shown;

[0012] Figure 4 yes Figure 2 A schematic flow chart of another exemplary embodiment of step S210 in the sample analysis method is shown;

[0013] Figure 5 yes Figure 2 A schematic flow chart of another exemplary embodiment of step S210 in the sample analysis method is shown;

[0014] Figure 6 yes Figure 2 A schematic flow chart of another exemplary embodiment of step S210 in the sample analysis method is shown;

[0015] Figure 7 It is a specific flow chart of an exemplary embodiment of the sample analysis method shown in the present application;

[0016] Figure 8 It is a schematic structural diagram of an exemplary embodiment of a sample analyzer shown in the present application. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. It will be appreciated that the specific embodiments described herein are only used to explain the present application, rather than to limit the present application. It should also be noted that, for ease of description, only some but not all structures related to the present application are shown in the drawings. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the art without making creative work are within the scope of protection of the present application.

[0018] In the description of the embodiments of the present application, technical terms such as "first", "second", "third", etc. are only used to distinguish different objects and cannot be understood as indicating or implying relative importance or implicitly indicating the number, specific order or primary and secondary relationship of the indicated technical features.

[0019] First of all, it should be noted that red blood cells and platelets are important components of blood cells, and accurate analysis of red blood cells and platelets in blood cells is of great significance for diagnosing and detecting diseases. Based on this, the embodiment of the present application can obtain more accurate red blood cell and platelet parameter results by combining optical channels and impedance channels to analyze blood cells.

[0020] Sample analysis methods refer to methods for detecting specific components or properties in a sample. Sample analysis methods mainly include optical methods and impedance methods. The optical method refers to a method of measuring and analyzing blood samples using optical principles. By measuring the scattering, absorption and transmission characteristics of light by different types of cells, information about the number, morphology and function of cells can be obtained. The impedance method refers to the measurement and analysis of blood samples based on the measurement principle of resistance. The blood sample is passed through a small channel, and the change in resistance is used to detect the presence of cells. The measurement and analysis are performed based on the characteristics of the resistance signal.

[0021] The sample analyzer is an instrument for detecting blood samples, including an optical channel, an impedance channel and a controller. When it is necessary to obtain the optical detection result of the blood sample by optical method, the blood sample is detected through the optical channel, and the light beam in the optical channel is used to make different cells in the blood sample produce different reactions, thereby obtaining the cell information in the blood sample. When it is necessary to obtain the impedance detection result of the blood sample by impedance method, the blood sample is detected through the impedance channel. The impedance channel is provided with electrodes. When the blood sample passes through the channel, the electrodes measure the resistance change generated by the cells passing through, thereby realizing the measurement and analysis of the blood sample.

[0022] See also Figure 1 , Figure 1 1 is a flow chart of an exemplary embodiment of a sample analysis method shown in the present application. Specifically, it may include the following steps:

[0023] S110: preparing a first sample solution and a second sample solution.

[0024] The first sample liquid may refer to the sample liquid detected in the optical channel. Exemplarily, the first sample liquid may be obtained by pre-treating a blood sample according to the detection environment of the optical channel. Exemplarily, the steps of pre-treating the blood sample may include blood collection, blood dilution, cell fixation, cell staining, etc.

[0025] The second sample liquid may refer to the sample liquid detected in the impedance channel. Exemplarily, the second sample liquid may be obtained by pre-treating the blood sample according to the detection environment of the impedance channel. Exemplarily, the steps of pre-treating the blood sample may include blood collection, mixing, etc.

[0026] It should be noted that the volumes of the first sample liquid and the second sample liquid may be the same or different, which will not affect the final detection result.

[0027] S120: Detecting the first sample liquid through an optical channel to obtain a first detection result, and detecting the second sample liquid through an impedance channel to obtain a second detection result.

[0028] The optical channel refers to a channel for detecting the first sample liquid using an optical detection method. Exemplarily, the optical channel may be composed of an optical detection part and a liquid path part, etc., and is used to separate and measure the optical information of red blood cells and platelets.

[0029] The first test result refers to the measurement result of red blood cells and platelets obtained by detecting blood cells through the optical channel. Exemplarily, the first test result can be obtained by analyzing the optical information of red blood cells and platelets in the blood cells through the optical channel.

[0030] The impedance channel refers to a channel for detecting the second sample liquid using an impedance method. For example, the impedance channel can calculate the impedance values ​​of red blood cells and platelets in blood cells by measuring current and voltage.

[0031] The second detection result refers to the measurement result of red blood cells and platelets obtained by detecting blood cells through the impedance channel. Exemplarily, the second detection result can be obtained by analyzing the impedance values ​​of red blood cells and platelets in blood cells through the impedance channel.

[0032] It can be understood that the first test result and the second test result are both measurement results of red blood cells and platelets, but the detection methods are different. The first test result is obtained by optical detection method, and the second test result is obtained by impedance method. Therefore, the test result values ​​obtained by different detection methods are not necessarily exactly the same, and the accuracy of the test results is also different.

[0033] S130: Determine the signal quality of the optical channel and the impedance channel based on the first detection result and the second detection result.

[0034] Signal quality refers to the accuracy of measuring red blood cells and platelets through an optical channel or an impedance channel. Exemplarily, the signal quality of the optical channel and the impedance channel can be determined by analyzing the detection quality of the first detection result and the second detection result.

[0035] It should be noted that the signal quality may be judged multiple times, and the criteria for each judgment may be different. For example, the signal quality may be judged once based on one of the first test result and the second test result, for example, the signal quality may be judged based on the particle number information of red blood cells and platelets within a preset time.

[0036] S140: Calculate the parameter results of red blood cells and platelets according to the signal quality.

[0037] Red blood cells are small cells found in the blood and are mainly composed of hemoglobin.

[0038] Platelets are tiny platelet-like cells found in the blood that are primarily involved in the formation of blood clots and the process of stopping bleeding.

[0039] It can be understood that the parameter results of red blood cells and platelets can be calculated based on the signal quality after judging the signal quality of the optical channel and the impedance channel, and then the parameter results of red blood cells and platelets are calculated based on the signal quality judgment result through the first test result and the second test result.

[0040] It can be seen that the sample analysis method of the embodiment of the present application prepares a first sample liquid and a second sample liquid; detects the first sample liquid through an optical channel to obtain a first detection result, and detects the second sample liquid through an impedance channel to obtain a second detection result; judges the signal quality of the optical channel and the impedance channel based on the first detection result and the second detection result; and calculates the parameter results of red blood cells and platelets according to the signal quality. In the above manner, the impedance channel and the optical channel of the present application do not perform another detection method based on the result of a certain detection method, but calculate the parameter results of red blood cells and platelets in combination with the detection results of the optical channel and the impedance channel, thereby avoiding the problem of a decrease in the measurement accuracy of red blood cells and platelets when an error occurs when using the impedance channel alone to judge an abnormality.

[0041] Based on the above embodiments, the embodiments of the present application adopt Figure 2 The flowchart details how the RBC and PLT parameter results are calculated based on the signal quality of the optical and impedance channels. Figure 2 , Figure 2 yes Figure 1 The flow chart of an exemplary embodiment of step S140 in the sample analysis method is shown. Specifically, in step S140, the process of calculating the parameter results of red blood cells and platelets according to the signal quality specifically includes the following steps:

[0042] S210: In response to the signal qualities of the optical channel and the impedance channel both reaching the standard, further determining the better signal quality of the optical channel and the impedance channel, and using the detection result corresponding to the better signal quality to calculate the parameter results of red blood cells and platelets.

[0043] Meeting the standard means that the optical channel or impedance channel meets the preset standard. Exemplarily, whether the standard is met can be determined by comparing the difference between the first test result and the second test result and the preset value. The preset value can be a predetermined optimal value.

[0044] Exemplarily, if the optical channel is judged to have better signal quality, the first detection result is used to calculate the parameter results of red blood cells and platelets; if the impedance channel is judged to have better signal quality, the second detection result is used to calculate the parameter results of red blood cells and platelets.

[0045] S220: In response to the signal quality of one of the optical channel and the impedance channel meeting the standard and the other not meeting the standard, the corresponding detection result of the meeting the standard is used to calculate the parameter results of the red blood cells and platelets.

[0046] Exemplarily, if the signal quality of the optical channel is judged to be up to standard, but the signal quality of the impedance channel is not up to standard, the first detection result is used to calculate the parameter results of red blood cells and platelets; if the signal quality of the impedance channel is judged to be up to standard, but the signal quality of the optical channel is not up to standard, the second detection result is used to calculate the parameter results of red blood cells and platelets.

[0047] S230: In response to the signal qualities of the optical channel and the impedance channel not being up to standard, calculating the parameter results of red blood cells and platelets by combining the first detection result and the second detection result.

[0048] Wherein, when the signal quality of both the optical channel and the impedance channel is not up to standard, the parameter results of the red blood cells and platelets can be calculated by combining the detection results of the optical channel and the impedance channel at a preset ratio. Exemplarily, the preset ratio can be determined according to the signal quality of the optical channel and the impedance channel, and the ratio of the detection results corresponding to the channel with relatively good signal quality can be greater than the detection results corresponding to the channel with relatively poor signal quality.

[0049] It can be seen that the sample analysis method of the embodiment of the present application calculates the parameter results of red blood cells and platelets by comparing the signal quality of the optical channel and the impedance channel. The corresponding test result with better signal quality in the optical channel and the impedance channel is selected to calculate the parameter results of red blood cells and platelets. If the signal quality of the optical channel and the impedance channel is not up to standard, the parameter results of red blood cells and platelets are calculated by combining the first test result and the second test result. In the prior art, the abnormal analysis of the impedance channel is first performed. Only when there is an abnormality will the optical channel be detected and the test result of the optical channel be adopted. If there is no abnormality in the impedance channel, the test result of the impedance channel is directly adopted. This will cause if the detection of the impedance channel is abnormal, but there is an error in this abnormality (the actual result may be no abnormality), or the test result of the optical channel is worse than the test result of the impedance channel, then the test result of the optical channel will cause the detection accuracy to be worse. Another possibility is that if the test result of the impedance channel is normal, but there is an error in this abnormality (the actual result may be abnormal), or the test result of the optical channel is better, then the test result of the impedance channel is used to lose the better test result. Compared with the prior art, the impedance channel and optical channel of the present application do not execute a certain detection method based on the result of another detection method, but calculate the parameter results of red blood cells and platelets by combining the detection results of the optical channel and the impedance channel, thereby avoiding the problem of reduced measurement accuracy of red blood cells and platelets when errors occur in judging abnormalities using the impedance channel alone.

[0050] Based on the above examples, please refer to Figure 3 , Figure 3 yes Figure 2 The flow chart of an exemplary embodiment of step S210 in the sample analysis method is shown. Specifically, step S210 further determines the better signal quality of the optical channel and the impedance channel, and specifically includes the following steps:

[0051] First of all, it should be noted that the first detection result includes the first particle number information within a preset time, and the second detection result includes the second particle number information within a preset time.

[0052] The first particle number information within the preset time refers to the number of particles of red blood cells and platelets detected within the preset time through the optical channel.

[0053] The second particle number information within the preset time refers to the number of red blood cells and platelets detected within the preset time through the impedance channel.

[0054] S310: Calculate a first variation coefficient by using first particle number information within a preset time, and calculate a second variation coefficient by using second particle number information within a preset time.

[0055] The first coefficient of variation is an indicator of the degree of variation of the first particle number information, which can reflect the stability of the optical channel detection. For example, the first standard deviation of red blood cells and platelets and the first average value of red blood cells and platelets can be calculated based on the first particle number information, and the ratio between the first standard deviation of red blood cells and platelets and the first average value of red blood cells and platelets can be used as the first coefficient of variation.

[0056] The second coefficient of variation is an indicator for measuring the degree of variation of the second particle number information, which can reflect the stability of the impedance channel detection. For example, the second standard deviation of red blood cells and platelets and the second average value of red blood cells and platelets can be calculated based on the second particle number information, and the ratio between the second standard deviation of red blood cells and platelets and the second average value of red blood cells and platelets can be used as the second coefficient of variation.

[0057] S320: In response to the first coefficient of variation being less than or equal to the first threshold, determining that the signal quality of the optical channel meets the standard.

[0058] The first threshold value may be a preset value. Exemplarily, if the first coefficient of variation is less than or equal to the first threshold value, it is determined that the signal quality of the optical channel meets the standard. On the contrary, if the first coefficient of variation is greater than the first threshold value, it is determined that the signal quality of the optical channel does not meet the standard.

[0059] S330: In response to the second coefficient of variation being less than or equal to the first threshold, determining that the signal quality of the impedance channel meets the standard.

[0060] It should be noted that, in this embodiment, when calculating red blood cells and platelets, the signal quality of the impedance channel and the optical channel needs to be combined, that is, the judgment results of step S320 and step S330 need to be combined and analyzed. Exemplarily, when the first coefficient of variation is less than or equal to the first threshold, and the second coefficient of variation is greater than the first threshold, the parameter results of red blood cells and platelets are calculated according to the first test result; when the first coefficient of variation is greater than the first threshold, and the second coefficient of variation is less than or equal to the first threshold, the parameter results of red blood cells and platelets are calculated according to the second test result; when the first coefficient of variation and the second coefficient of variation are both less than or equal to the first threshold, it is necessary to further determine the better one of the optical channel and the impedance channel; when the first coefficient of variation and the second coefficient of variation are both greater than the first threshold, the parameter results of red blood cells and platelets are calculated based on the first test result and the second test result.

[0061] It can be seen that the sample analysis method of the embodiment of the present application calculates the first coefficient of variation through the first particle number information within the preset time, and calculates the second coefficient of variation through the second particle number information within the preset time; in response to the first coefficient of variation being less than or equal to the first threshold, it is judged that the signal quality of the optical channel meets the standard; in response to the second coefficient of variation being less than or equal to the first threshold, it is judged that the signal quality of the impedance channel meets the standard. Therefore, the signal quality is judged by combining the particle number information detection results of the optical channel and the impedance channel within the preset time, and the parameter results of the red blood cells and platelets are calculated according to the signal quality, which can improve the accuracy of the red blood cell and platelet measurement.

[0062] Based on the above examples, please refer to Figure 4 , Figure 4 yes Figure 2 The flowchart of another exemplary embodiment of step S210 in the sample analysis method is shown. Specifically, step S210 further determines the better signal quality of the optical channel and the impedance channel and specifically includes the following steps:

[0063] First of all, it should be noted that the first test result also includes the volume information and fluorescence intensity of red blood cells and platelets.

[0064] The volume information reflects the size and morphological characteristics of red blood cells and platelets. Exemplarily, the volume information can be obtained by detecting blood cells through an optical channel.

[0065] Fluorescence intensity refers to the intensity of the fluorescent signal generated by red blood cells and platelets. For example, fluorescence intensity refers to the brightness of light presented by red blood cells and platelets labeled with fluorescent dyes under light irradiation.

[0066] It should be noted that the premise for the judgment of this embodiment is that the judgment results of the optical channel and the impedance channel through the particle number information within the preset time are both up to standard. That is, if the judgment result of the previous embodiment is that one channel is up to standard and the other is not up to standard, or both channels are not up to standard, the calculation of red blood cells and platelets is directly performed based on the judgment result of the previous embodiment, and the judgment of this embodiment is not required.

[0067] S410: Draw a two-dimensional scatter plot using volume information and fluorescence intensity.

[0068] The two-dimensional scatter plot is used to show the relationship between volume information and fluorescence intensity. For example, in order to facilitate the observation of the measurement results of red blood cells and platelets, the volume information and fluorescence intensity of red blood cells and platelets are plotted on the same two-dimensional scatter plot.

[0069] Since the volume and number of red blood cells and platelets in blood cells are different, the actual volume information and the distribution area of ​​the actual fluorescence intensity of red blood cells and platelets in the two-dimensional scatter plot are different. This application draws a two-dimensional scatter plot based on the measurement results of the optical channel, which can more intuitively show the detection effect of the optical channel and further determine the signal quality of the optical channel.

[0070] S420: Counting the number of scatter points of red blood cells and platelets based on the two-dimensional scatter plot.

[0071] The number of scatter points refers to the coordinate points of the volume information and the fluorescence intensity in the first detection result in the two-dimensional scatter plot. Exemplarily, the number of scatter points of red blood cells in the two-dimensional scatter plot and the number of scatter points of platelets in the two-dimensional scatter plot are counted respectively.

[0072] S430: Compare the overlapping area of ​​the scattered point counts of red blood cells and platelets with a second threshold.

[0073] The overlapping area refers to the volume information or fluorescence intensity of red blood cells and platelets at the same coordinate point in the two-dimensional scatter plot, that is, the volume information or fluorescence intensity of red blood cells and platelets are the same. Exemplarily, the overlapping area of ​​red blood cells and platelets can be obtained by counting the number of scatter points of red blood cells and platelets. It should be noted that the overlapping area reflects the effect of the optical channel in distinguishing red blood cells and platelets when detecting blood cells, and further indicates the signal quality of the optical channel.

[0074] The second threshold value may be a preset value. Exemplarily, the second threshold value may be manually predetermined.

[0075] S440: In response to the overlapping area being less than or equal to the second threshold, it is determined that the signal quality of the optical channel meets the standard.

[0076] It should be noted that in this judgment step, since the detection effect of the optical channel is usually better than that of the impedance channel, in this judgment step, if the signal quality of the optical channel meets the standard, the first detection result corresponding to the optical channel is directly used to calculate the parameter results of red blood cells and platelets without judging the signal quality of the impedance channel.

[0077] It can be seen that the sample analysis method of the embodiment of the present application draws a two-dimensional scatter plot through volume information and fluorescence intensity; counts the scatter points of red blood cells and platelets based on the two-dimensional scatter plot; compares the overlapping area of ​​the scatter points of red blood cells and platelets with the second threshold; in response to the overlapping area being less than or equal to the second threshold, it is judged that the signal quality of the optical channel meets the standard. Therefore, when the overlapping area is less than or equal to the second threshold, it indicates that the optical channel can better distinguish between red blood cells and platelets, that is, the signal quality of the optical channel meets the standard. Determining how to calculate red blood cells and platelets only by the signal quality of the optical channel simplifies the judgment process and improves the efficiency of blood cell analysis.

[0078] Based on the above examples, please refer to Figure 5 , Figure 5 yes Figure 2 The flowchart of another exemplary embodiment of step S210 in the sample analysis method is shown. Specifically, step S210 further determines the better signal quality of the optical channel and the impedance channel and specifically includes the following steps:

[0079] Firstly, it should be noted that the second test result also includes the volume distribution information of red blood cells and platelets.

[0080] The volume distribution information refers to the volume distribution of red blood cells and platelets. For example, the volume distribution information can be obtained by detecting the impedance information of blood cells through an impedance channel.

[0081] S510: In response to the overlapping area being greater than a second threshold, obtaining a volume distribution histogram of the optical channel based on the two-dimensional scatter plot, and obtaining a volume distribution histogram of the impedance channel based on the volume distribution information.

[0082] It should be noted that the premise for the judgment of this embodiment is that the judgment result of the optical channel signal quality by the two-dimensional scatter plot drawn according to the volume information and the fluorescence intensity in the previous embodiment does not meet the standard, that is, the overlap area is greater than the second threshold value, and the judgment of this embodiment will be performed. That is, if the signal quality of the optical channel is judged to meet the standard according to the two-dimensional scatter plot, the parameter results of red blood cells and platelets are directly calculated according to the judgment result of the previous embodiment, and the judgment of this embodiment is not required.

[0083] The volume distribution histogram is a graph used to describe the distribution of the number of red blood cells and platelets in different volume ranges. Exemplarily, the two-dimensional scatter plot includes volume information of red blood cells and platelets measured according to the optical channel, and the volume distribution histogram of the optical channel is obtained from the volume information in the two-dimensional scatter plot.

[0084] S520: Calculate the first distribution width of red blood cells and platelets through the volume distribution histogram of the optical channel, and calculate the second distribution width of red blood cells and platelets through the volume distribution histogram of the impedance channel.

[0085] The distribution width is used to describe the distribution of red blood cells and platelets in different volume ranges. Exemplarily, the first distribution width is calculated by the volume distribution histogram of the optical channel, and the second distribution width is calculated by the volume distribution histogram of the impedance channel.

[0086] S530: In response to the difference between the first distribution width and the second distribution width being less than or equal to a third threshold, determining that the signal quality of the impedance channel is a better one.

[0087] The third threshold value may be a preset value. Exemplarily, the third threshold value may be manually preset. Since the detection accuracy of the optical channel is generally higher than the detection accuracy of the impedance channel, the first distribution width of the optical channel is used as a standard. If the difference between the first distribution width and the second distribution width is smaller, it means that the difference between the impedance channel and the optical channel is smaller, and the signal quality of the impedance channel is better.

[0088] It can be seen that the sample analysis method of the embodiment of the present application judges the signal quality of the impedance channel through the first distribution width of the optical channel and the second distribution width of the impedance channel, and determines how to calculate red blood cells and platelets based on the signal quality of the impedance channel. In this way, it is possible to determine whether to use the impedance channel for calculation under the standard of the optical channel, thereby achieving the effect of the optical channel correcting the impedance channel.

[0089] Based on the above examples, please refer to Figure 6 , Figure 6 yes Figure 2 The flowchart of another exemplary embodiment of step S210 in the sample analysis method is shown. Specifically, step S210 further determines the better signal quality of the optical channel and the impedance channel and specifically includes the following steps:

[0090] In response to the difference between the first distribution width and the second distribution width being greater than the third threshold and the first distribution width being less than or equal to the fourth threshold, it is determined that the signal quality of the optical channel is a better one.

[0091] The fourth threshold value may be a preset value. Exemplarily, the fourth threshold value may be preset manually.

[0092] It should be noted that the premise for the judgment of this embodiment is that the difference between the first distribution width and the second distribution width in the previous embodiment is greater than the third threshold value, that is, the signal quality of the impedance channel is not up to standard, and the judgment step of this embodiment will be performed. If the signal quality of the impedance channel in the previous embodiment is up to standard, the parameter results of red blood cells and platelets are directly calculated by the judgment result of the previous embodiment, that is, the second detection result corresponding to the impedance channel.

[0093] If the first distribution width is less than or equal to the fourth threshold value, the signal quality of the optical channel is better, and the first test result corresponding to the optical channel is directly used to calculate the parameter results of red blood cells and platelets; if the first distribution width is greater than the fourth threshold value, the signal quality of the optical channel does not meet the standard, and the parameter results of red blood cells and platelets are calculated by combining the first test result and the second test result.

[0094] Furthermore, the parameter results of red blood cells and platelets include parameter results calculated from at least one parameter or a combination of red blood cell count, platelet count, mean red blood cell volume, mean platelet volume, red blood cell distribution width, and platelet distribution width.

[0095] Exemplarily, the parameter results of red blood cells and platelets can be obtained by weighted calculation based on the detection results corresponding to the optical channel and the impedance channel.

[0096] Furthermore, the first sample liquid is a mixture of a dedicated fluorescent dye, a sample and an auxiliary reaction liquid according to a preset ratio, and the second sample liquid is a mixture of a sample and an auxiliary reaction liquid.

[0097] A dedicated fluorescent dye refers to a compound that emits fluorescence under certain conditions. For example, the optical channel needs to collect the fluorescence intensity of red blood cells and platelets, so the red blood cells and platelets need to be stained with a dedicated fluorescent dye to obtain the fluorescence intensity of the red blood cells and platelets.

[0098] The sample refers to a collected blood sample. Exemplarily, the sample volume in the first sample solution may be the same as or different from the sample volume in the second sample solution, which is not specifically limited herein.

[0099] The auxiliary reaction liquid refers to a solution that promotes the sample to react in the corresponding channel. For example, the auxiliary reaction liquid in the first sample liquid can be the same as the auxiliary reaction liquid in the second sample liquid, or can be different, which is not specifically limited here.

[0100] The process of mixing the first sample liquid and the process of mixing the second sample liquid can be the same or different. The order of the process does not affect the detection effect of the impedance channel and the optical channel.

[0101] It can be seen that preparing different sample solutions according to the characteristics of different channels can make the samples have different reaction effects in the impedance channel and the optical channel, thereby obtaining more accurate detection results.

[0102] Furthermore, the optical channel and the impedance channel operate simultaneously or at least partially overlap.

[0103] Exemplarily, the optical channel and the impedance channel can start the detection of blood cells at the same time, or one of the optical channel and the impedance channel can start first and the other one can start later, as long as the detection results of the optical channel and the impedance channel can be obtained.

[0104] It can be seen that in the sample analysis method of the embodiment of the present application, the optical channel and the impedance channel work simultaneously or at least partially overlap in time, which can reduce the error of sample analysis.

[0105] In order to elaborate on the sample analysis method of this application, Figure 7 The flowchart shown further illustrates it, and the details are as follows:

[0106] The prepared first sample liquid and the second sample liquid are detected through the optical channel and the impedance channel respectively to obtain the first detection result and the second detection result, and the first coefficient of variation is calculated based on the first particle number information within the preset time, and the second coefficient of variation is calculated based on the second particle number information within the preset time. The first coefficient of variation and the second coefficient of variation are respectively compared with the first threshold value. If the first coefficient of variation and the second coefficient of variation are both greater than the first threshold value, the parameter results of red blood cells and platelets are calculated by combining the first detection result and the second detection result. If one of the first coefficient of variation and the second coefficient of variation is greater than the first threshold value and the other is less than or equal to the first threshold value, the parameter results of red blood cells and platelets are calculated according to the detection result corresponding to the channel less than or equal to the first threshold value. If the first coefficient of variation and the second coefficient of variation are both less than or equal to the first threshold value, the scattered point counts of red blood cells and platelets are calculated through a two-dimensional scatter plot drawn by volume information and fluorescence intensity. If the overlapping area of ​​the scatter points of red blood cells and platelets is less than or equal to the second threshold, the parameter results of red blood cells and platelets are calculated according to the first test result; if the overlapping area of ​​the scatter points of red blood cells and platelets is greater than the second threshold, the first distribution width is obtained based on the two-dimensional scatter plot, and the second distribution width is obtained based on the volume distribution information. If the difference between the first distribution width and the second distribution width is less than or equal to the third threshold, the parameter results of red blood cells and platelets are calculated according to the second test result. If the difference between the first distribution width and the second distribution width is greater than the third threshold, the first distribution width is compared with the fourth threshold. If the first distribution width is less than or equal to the fourth threshold, the parameter results of red blood cells and platelets are calculated according to the first test result, and if the first distribution width is greater than the fourth threshold, the parameter results of red blood cells and platelets are calculated based on the first test result and the second test result.

[0107] See also Figure 8 , Figure 8 8 is a schematic diagram of an exemplary embodiment of a sample analyzer shown in the present application. The sample analyzer 80 includes an optical channel 810, an impedance channel 820 and a controller 830. The controller 830 is connected to the optical channel 810 and the impedance channel 820 respectively; the optical channel 810 is used to detect the first detection result of red blood cells and platelets; the impedance channel 820 is used to detect the second detection result of red blood cells and platelets; the controller 830 is used to determine the signal quality of the optical channel 810 and the impedance channel 820, and calculate the parameter results of red blood cells and platelets according to the signal quality.

[0108] The optical channel 810 includes an optical detection part and a fluid path part, and is used to detect optical information of red blood cells and platelets.

[0109] The impedance channel 820 is used to detect the impedance information of red blood cells and platelets.

[0110] The controller 830 is used to determine the signal quality of the optical channel 810 and the impedance channel 820 and calculate the parameter results of the red blood cells and platelets. Exemplarily, determining the signal quality of the optical channel 810 and the impedance channel 820 includes determining whether the coefficient of variation calculated based on the particle number information within a preset time exceeds a first threshold, whether the overlapping area of ​​the scattered point numbers of red blood cells and platelets exceeds a second threshold, whether the difference between the first distribution width of the optical channel 810 and the second distribution width of the impedance channel 820 exceeds a third threshold, whether the first distribution width of the optical channel 810 exceeds a fourth threshold, and whether the overlapping area of ​​the two-dimensional signal of the optical channel 810 exceeds a preset threshold, etc.

[0111] In the above scheme, the sample analyzer 80 of the embodiment of the present application detects the first sample liquid through the optical channel 810 to obtain the first detection result, and detects the second sample liquid through the impedance channel 820 to obtain the second detection result; based on the first detection result and the second detection result, the signal quality of the optical channel 810 and the impedance channel 820 is determined; according to the signal quality, the parameter results of the red blood cells and platelets are calculated. Therefore, the combination of the optical channel 810 and the impedance channel 820 can improve the accuracy of the red blood cell and platelet measurement.

[0112] The above description is only an implementation method of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A sample analysis method, characterized in that: The method comprises: preparing a first sample solution and a second sample solution; Detecting the first sample liquid through an optical channel to obtain a first detection result, and detecting the second sample liquid through an impedance channel to obtain a second detection result; Determine the signal quality of the optical channel and the impedance channel based on the first detection result and the second detection result; Based on the signal quality, the parameter results of red blood cells and platelets are calculated.

2. The sample analysis method according to claim 1, characterized in that: The calculation of the red blood cell and platelet parameter results according to the signal quality includes: In response to the signal qualities of the optical channel and the impedance channel both meeting the standards, further determining a better one of the signal qualities of the optical channel and the impedance channel, and using the detection result corresponding to the better one to calculate the parameter results of the red blood cells and platelets; In response to the signal quality of one of the optical channel and the impedance channel meeting the standard and the other not meeting the standard, the corresponding detection result of the meeting the standard is used to calculate the parameter results of the red blood cells and platelets; In response to the signal qualities of the optical channel and the impedance channel not being up to standard, the parameter results of the red blood cells and platelets are calculated by combining the first detection result and the second detection result.

3. The sample analysis method according to claim 2, characterized in that: The first detection result includes first particle number information within a preset time, and the second detection result includes second particle number information within a preset time; The further determining the better one of the signal qualities of the optical channel and the impedance channel comprises: A first coefficient of variation is calculated by first particle number information within the preset time, and a second coefficient of variation is calculated by second particle number information within the preset time; In response to the first coefficient of variation being less than or equal to a first threshold, determining that the signal quality of the optical channel meets the standard; In response to the second coefficient of variation being less than or equal to a first threshold, it is determined that the signal quality of the impedance channel meets the standard.

4. The sample analysis method according to claim 2, characterized in that: The first detection result includes volume information and fluorescence intensity of the red blood cells and platelets; The further determining the better one of the signal qualities of the optical channel and the impedance channel comprises: Drawing a two-dimensional scatter plot using the volume information and the fluorescence intensity; Counting the scatter points of the red blood cells and the platelets based on the two-dimensional scatter plot; comparing the overlapping area of ​​the scatter point counts of the red blood cells and the platelets with a second threshold; In response to the overlapping area being less than or equal to the second threshold, it is determined that the signal quality of the optical channel meets the standard.

5. The sample analysis method according to claim 4, characterized in that: The second test result includes volume distribution information of the red blood cells and platelets; The further determining the better one of the signal qualities of the optical channel and the impedance channel comprises: In response to the overlapping area being greater than the second threshold, Obtaining a volume distribution histogram of the optical channel based on the two-dimensional scatter plot, and obtaining a volume distribution histogram of the impedance channel based on the volume distribution information; The first distribution widths of the red blood cells and the platelets are calculated by the volume distribution histogram of the optical channel, and the second distribution widths of the red blood cells and the platelets are calculated by the volume distribution histogram of the impedance channel; In response to the difference between the first distribution width and the second distribution width being less than or equal to a third threshold, it is determined that the signal quality of the impedance channel is a better one.

6. The sample analysis method according to claim 5, characterized in that: The further determining the better one of the signal qualities of the optical channel and the impedance channel comprises: In response to the difference between the first distribution width and the second distribution width being greater than a third threshold and the first distribution width being less than or equal to a fourth threshold, it is determined that the signal quality of the optical channel is a better one.

7. The sample analysis method according to claim 1, characterized in that: The parameter results of red blood cells and platelets include red blood cell count, platelet count, mean red blood cell volume, mean platelet volume, red blood cell distribution width, platelet distribution width, at least one parameter or a combination of the calculated parameters.

8. The sample analysis method according to claim 1, characterized in that: The first sample liquid is a mixture of a dedicated fluorescent dye, a sample and an auxiliary reaction liquid according to a preset ratio, and the second sample liquid is a mixture of a sample and an auxiliary reaction liquid.

9. The sample analysis method according to claim 1, characterized in that: The optical channel and the impedance channel operate simultaneously or at least partially overlap.

10. A sample analyzer, characterized in that: include: Optical channel, impedance channel, controller; Wherein, the controller is connected to the optical channel and the impedance channel respectively; The optical channel is used to detect a first detection result of red blood cells and platelets; The impedance channel is used to detect the second detection result of the red blood cells and platelets; The controller is used to determine the signal quality of the optical channel and the impedance channel, and calculate the parameter results of the red blood cells and platelets according to the signal quality.

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