Micro-fluidic chip

Abstract

The present invention relates to the technical field of microfluidics, and discloses a microfluidic chip, which comprises a mixing detection module, a control valve and a power module, and the mixing detection module comprises a plurality of storage cavities, a first channel, a second channel, a third channel, a common channel, a mixing cavity and a detection cavity. According to the invention, the storage cavity for providing the reagent, the channel for fluid such as the reagent and the sample to flow, the mixing cavity for mixing and reacting the fluid such as the reagent and the sample, and the detection cavity for analyzing and detecting the sample are integrated on the mixing detection module; a control valve and a power module which are used for realizing directional flow and mixed reaction of fluid in each channel are arranged as parts which can be removed relative to the mixed detection module, so that the integration degree of the micro-fluidic chip can be improved, and the volume of the micro-fluidic chip can be reduced; in addition, the installation and replacement of the mixed detection module serving as a consumable are facilitated, and the flexibility and convenience of the micro-fluidic chip in use are effectively improved.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic technology, and in particular to a microfluidic chip. Background Art

[0002] The contents in this section merely provide background information related to the present invention and may not constitute prior art.

[0003] Microfluidic chip technology, as a sample analysis technology with functions such as sample sampling, reaction, mixing, and detection, is widely used in medical, biochemical and other fields, especially in automated biochemical analyzers.

[0004] However, the modules of known microfluidic chips for realizing different functions usually adopt a split design. Such microfluidic chips are not only bulky, but also the process of installing and replacing consumables is cumbersome. Summary of the invention

[0005] In view of this, an object of the present invention is to provide a microfluidic chip with a high degree of integration, a small size, and convenient installation and replacement of consumables.

[0006] The purpose of the present invention is achieved through the following technical solutions:

[0007] The present invention discloses a microfluidic chip, comprising a mixing detection module, a control valve and a power module;

[0008] The hybrid detection module comprises:

[0009] A plurality of storage chambers suitable for storing reagents, each of the storage chambers being equipped with a first channel;

[0010] a second channel, wherein a mixing chamber is provided on the second channel;

[0011] A detection chamber, connected to the mixing chamber;

[0012] at least one third channel;

[0013] Public access;

[0014] The control valve is removably attached to the mixing detection module; wherein, when the control valve is attached to the mixing detection module, the control valve is configured to selectively allow the common channel to communicate with any one of the first channel, the second channel or the third channel;

[0015] The power module is removably attached to the hybrid detection module; wherein, when the power module is attached to the hybrid detection module, the power module is connected to the common channel so as to pressurize or depressurize the common channel through the power module.

[0016] Furthermore, the mixed detection module further comprises a storage box and a base plate, wherein the storage box and the base plate are arranged to overlap each other;

[0017] The plurality of storage cavities are disposed in the storage box; the first channel, the second channel, the third channel and the common channel are disposed in the substrate;

[0018] The storage box and the base plate are detachably connected; wherein, when the base plate is connected to the storage box, the first channel is connected to the corresponding storage cavity;

[0019] The control valve and the power module are removably attached to a side of the base plate facing away from the storage box.

[0020] Furthermore, a first connecting column is provided on a side of the base plate facing the storage box, the first connecting column corresponds to the first channel one by one and extends toward the storage box, and a first end of the first channel passes through an end of the first connecting column;

[0021] The storage box is provided with a first groove corresponding to the storage cavity one by one on one side facing the base plate, and the first groove is provided with an outlet communicating with the corresponding storage cavity;

[0022] The first connecting column is inserted into the corresponding first groove, and a first sealing member is provided between the outer wall of the first connecting column and the inner wall of the first groove.

[0023] Furthermore, the storage cavity has an inlet, and a sealing portion is provided at the inlet;

[0024] The sealing portion is configured to prevent the reagent in the storage cavity from flowing out from the inlet and maintain the air pressure balance between the interior of the storage cavity and the external environment.

[0025] Further, the hybrid detection module is provided with a mounting surface, the first channel, the second channel, the third channel and the second end of the common channel all pass through the mounting surface, and the second ends of the first channel, the second channel and the third channel are arranged on the same circumference with the second end of the common channel as the center;

[0026] The control valve comprises a rotating member and a positioning member, wherein the rotating member has a sealing surface and a flow guide channel; the sealing surface is in sealing cooperation with the mounting surface, the first end of the flow guide channel penetrates the sealing surface and is always in communication with the second end of the common channel, and the second end of the flow guide channel penetrates the sealing surface and is located on the circumference;

[0027] Wherein, the rotating member is configured to be able to rotate around the center of the circle;

[0028] The positioning member is configured to restrict the rotating member to the mounting surface and allow the rotating member to rotate around the center of the circle.

[0029] Furthermore, the mounting surface is provided with a clamping piece, and the clamping piece defines a mounting cavity enclosing each of the first channel, the second channel, the third channel and the second end of the common channel;

[0030] The positioning member includes a shell and an elastic member; the shell defines a receiving cavity, and the shell is snap-connected to the clamping member;

[0031] The rotating member and the elastic member are both arranged in the accommodating cavity; the elastic member is configured to provide the rotating member with an elastic force that forces the sealing surface to seal with the mounting surface and allows the rotating member to rotate.

[0032] Furthermore, the control valve further comprises a power source, and the power source is used to provide power to the rotating member to force it to rotate;

[0033] The power source includes a connecting member, a transmission mechanism and a motor; the connecting member is coaxially arranged with the rotating member;

[0034] The transmission mechanism includes a transmission shaft, a transmission gear set and a worm; the transmission shaft is coaxially connected to the connecting member, the worm is transmission-connected to the output end of the motor, and the worm is transmission-connected to the transmission shaft through the transmission gear set.

[0035] Further, the power module includes an adapter and a pump, the adapter is provided with a guide channel and a second groove corresponding to the guide channel one by one, the first end of the guide channel is located in the second groove, and the second end of the guide channel is connected to the output end of the pump;

[0036] The mounting surface is provided with second connecting columns corresponding to the second grooves one by one, the second connecting columns extend in a direction away from the mounting surface, and the common channel is provided with a connecting port penetrating through the ends of the second connecting columns;

[0037] The second connecting column is inserted into the corresponding second groove, and a second sealing member is provided between the outer wall of the second connecting column and the inner wall of the second groove.

[0038] Furthermore, there are two guide channels, and the pump includes a first pump and a second pump. The output end of the first pump is connected to the second end of one of the guide channels, and the output end of the second pump is connected to the second end of the other guide channel.

[0039] Furthermore, the hybrid detection module also includes a connection block and a detection box;

[0040] The connection block is arranged on the mounting surface, and a drainage channel corresponding to the third channel is arranged on the connection block; the detection box is detachably connected to the connection block, and the detection chamber is defined in the detection box, and the detection chamber is provided with a liquid inlet;

[0041] The first end of the second channel passes through the mounting surface and is communicated with the liquid inlet; the first end of the third channel passes through the mounting surface and is communicated with the first end of the corresponding drainage channel.

[0042] The technical solution of the embodiment of the present invention has at least the following advantages and beneficial effects:

[0043] 1. The microfluidic chip disclosed in the present invention integrates a storage cavity for providing reagents, a channel for the flow of fluids such as reagents and samples, a mixing cavity for the mixed reaction of fluids such as reagents and samples, and a detection cavity for analyzing and detecting samples on a mixing detection module, and sets a control valve and a power module for realizing directional flow and mixed reaction of fluids in each channel as removable components relative to the mixing detection module. This not only improves the integration level of the microfluidic chip to reduce the volume of the microfluidic chip, but also helps to install and replace the mixing detection module as a consumable, effectively improving the flexibility and convenience of the microfluidic chip when used.

[0044] 2. The present invention arranges a mixing chamber for mixing reactions of fluids such as reagents and samples on the second channel. Compared with the method of arranging the mixing chamber on the common channel, it can effectively reduce the residual amount of fluids such as reagents and samples in the common channel, thereby helping to reduce the burden of subsequent cleaning of each channel and improve the cleaning quality.

[0045] 3. The present invention utilizes a power module to provide power for the mixing reaction of reagents and samples in the mixing chamber. Compared with the method of using an additional mixing device to mix the reagents and samples, it can effectively reduce costs and further reduce the volume of the microfluidic chip. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 A schematic diagram of the structure of a microfluidic chip provided by an embodiment of the present invention;

[0047] Figure 2 A schematic diagram of the structure of a hybrid detection module provided by an embodiment of the present invention;

[0048] Figure 3 for Figure 2 An exploded view of the structure of the hybrid detection module shown in FIG.

[0049] Figure 4An exploded view of the structure of a storage box provided by an embodiment of the present invention;

[0050] Figure 5 An exploded view of the structure of the substrate provided by the embodiment of the present invention;

[0051] Figure 6 A front view of a second plate provided in an embodiment of the present invention, showing the distribution of channels in the hybrid detection module;

[0052] Figure 7 It is a partial structural cross-sectional view of the connection between the second channel and the detection cavity;

[0053] Figure 8 A cross-sectional view of a local structure of a connection between a single storage cavity and a first channel;

[0054] Fig. 9 A schematic diagram of the structure of a mixing detection module and a control valve provided in an embodiment of the present invention;

[0055] Fig.10 A schematic diagram of the structure of a control valve provided by an embodiment of the present invention;

[0056] Fig.11 for Fig.10 A cross-sectional view of a control valve shown in FIG.

[0057] Fig.12 A schematic diagram of the structure of a hybrid detection module and a power module provided in an embodiment of the present invention;

[0058] Fig.13 A schematic diagram of the structure of an adapter provided in an embodiment of the present invention;

[0059] Fig.14 It is a schematic diagram of the local structure of the connection between the guide channel on the adapter and the connection port of the common channel;

[0060] Fig.15 A schematic diagram of the structure of a connection block provided by an embodiment of the present invention;

[0061] Fig.16 It is a partial structural cross-sectional view of the connection between a single third channel and the drainage channel on the connection block;

[0062] Fig.17 An exploded view of the structure of the detection box provided by an embodiment of the present invention.

[0063] Icons: 10-mixed detection module, 11-storage chamber, 111-export, 112-import, 12-first channel, 13-second channel, 14-detection chamber, 141-liquid discharge port, 142-liquid inlet, 15-mixing chamber, 16-third channel, 17-common channel, 171-connection port, 18-storage box, 181-first female buckle, 182-first groove, 183-first box body, 184-first cover plate, 19-substrate, 191-first male buckle, 192-first connecting column, 193-first sealing member, 194-first plate body, 195-second plate body, 110-mounting surface, 120-clamping member, 121-mounting chamber, 122-second male buckle, 130-second connecting column, 140- The second sealing member, 150-connecting block, 151-drainage channel, 160-detection box, 161-second box body, 162-second cover plate, 20-control valve, 21-rotating member, 211-sealing surface, 212-drainage channel, 22-positioning member, 221-housing, 222-elastic member, 223-accommodating chamber, 224-second female buckle, 23-power source, 231-connecting member, 232-transmission mechanism, 2321-transmission shaft, 2322-transmission gear set, 2323-worm, 233-motor, 24-detection component, 241-code disk, 242-sensor, 30-power module, 31-adapter, 311-guide channel, 312-second groove, 32-first pump, 33-second pump. DETAILED DESCRIPTION

[0064] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described in conjunction with specific implementation methods below. The same figure marks in the accompanying drawings represent the same components. It should be noted that the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the described embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0065] Compared to the embodiments shown in the drawings, feasible embodiments within the scope of the present invention may have fewer components, other components not shown in the drawings, different components, differently arranged components, or differently connected components, etc. In addition, two or more components in the drawings may be implemented in a single component, or a single component shown in the drawings may be implemented as multiple separate components.

[0066] The embodiments of the present invention disclose a microfluidic chip, in particular a microfluidic chip with high integration, small size and convenient installation and replacement of consumables.

[0067] Figure 1 FIG. 2 shows the appearance of an exemplary microfluidic chip disclosed in an embodiment of the present invention. Figure 1 As shown, the microfluidic chip may include a mixing detection module 10 , a control valve 20 and a power module 30 .

[0068] In some embodiments, the mixed detection module 10 is used to provide various reagents required for sample analysis, provide a channel for the flow of fluids such as samples and reagents, and provide a place for the mixed reaction and detection of samples and reagents.

[0069] Combination Figure 4 , Figures 6 to 8 As shown, the hybrid detection module 10 may include a plurality of storage chambers 11. Each storage chamber 11 is suitable for storing reagents required for sample analysis. In addition, each storage chamber 11 is equipped with a first channel 12, and the reagents in each storage chamber 11 are suitable for entering the corresponding first channel 12.

[0070] The hybrid detection module 10 may further include a second channel 13 and a detection chamber 14. The second channel 13 is at least used to receive the reagent from the storage chamber 11. At the same time, the second channel 13 can also receive the sample to be analyzed, as well as fluids such as cleaning fluid to be described later. The cleaning fluid described in this embodiment may be, but is not limited to, water.

[0071] The second channel 13 is also provided with Figure 6 As shown in the mixing chamber 15, fluids such as reagents and samples entering the second channel 13 can enter the mixing chamber 15, so that the fluids such as reagents and samples can be mixed and reacted in the mixing chamber 15. Therefore, the mixing chamber 15 should have a large volume so that the mixing chamber 15 can accommodate enough reagents and samples.

[0072] The detection chamber 14 is connected to the mixing chamber 15. The detection chamber 14 is located downstream of the mixing chamber 15. When the reagents, samples and other fluids are mixed and reacted in the mixing chamber 15 to form a mixed fluid, the mixed fluid in the mixing chamber 15 can enter the detection chamber 14, so as to detect the sample in the mixed fluid in the detection chamber 14.

[0073] The hybrid detection module 10 may also include at least one third channel 16. The third channel 16 is used to guide samples that need to be analyzed from the outside, or cleaning fluids required for subsequent cleaning of each channel, or air in the external environment into the hybrid detection module 10, so as to realize functions such as sample analysis, channel cleaning, and channel pressure balance. In other words, the third channel 16 is a spare channel, which can be connected to different external devices according to the functions to be realized. For example, when sample analysis is required, the third channel 16 can be connected to an external sample source; when channel cleaning is required, the third channel 16 can be connected to an external cleaning fluid source; when the internal pressure of each channel needs to be balanced, the third channel 16 can be directly connected to the external atmosphere.

[0074] In order to improve the usability of the microfluidic chip, a plurality of third channels 16 may be provided for backup if space in the mixing detection module 10 permits.

[0075] The mixed detection module 10 further includes a common channel 17 , which serves as a transfer station for the above-mentioned reagents, samples and other fluids, so that the samples and reagents from the storage chamber 11 can enter the second channel 13 in sequence and reach the mixing chamber 15 .

[0076] For example, Figure 6 FIG. 2 shows a schematic diagram of possible distribution of each channel in the hybrid detection module 10 disclosed in the embodiment of the present invention. Figure 6 As shown, the hybrid detection module 10 may have 12 first channels 12 to correspond to 12 storage chambers 11 for storing different reagents, and the 12 first channels 12 may be arranged in a fan-shaped manner on one side in sequence to optimize the layout design of the channel. The second channel 13 may be arranged opposite to the 12 first channels 12, and the mixing chamber 15 on the second channel 13 may be in an "8" shape so that the mixing chamber 15 has a sufficiently large volume. The third channel 16 may be set to 5, and the 5 third channels 16 may be distributed on both sides of the second channel 13 to further optimize the layout design of the channel. The common channel 17 may be a meandering special-shaped structure to increase the volume of the common channel 17 without increasing the cross-sectional area of ​​the common channel 17.

[0077] In some embodiments, the control valve 20 is mainly used to achieve directional flow of reagents, samples, cleaning fluids and other fluids. For example, the reagents in the storage chamber 11 are allowed to enter the second channel 13 through the first channel 12 and the common channel 17 in sequence, or the samples, cleaning fluids and other fluids are allowed to enter the second channel 13 through the third channel 16 and the common channel 17 in sequence.

[0078] Specifically, the control valve 20 is a component that is removably attached to the mixing detection module 10. In the state where the control valve 20 is attached to the mixing detection module 10, the control valve 20 is configured to selectively allow the common channel 17 to communicate with any one of the first channel 12, the second channel 13, or the third channel 16. In other words, when the control valve 20 is attached to the mixing detection module 10, any one of the first channel 12, the second channel 13, or the third channel 16 can communicate with the common channel 17 under the control of the control valve 20, thereby realizing the switching of the flow path.

[0079] Thus, for a single storage chamber 11 and its corresponding first channel 12, when the control valve 20 controls the first channel 12 of the storage chamber 11 to be connected to the common channel 17, the reagent in the storage chamber 11 can enter the common channel 17 through the first channel 12. On this basis, it is only necessary to control the common channel 17 to be connected to the second channel 13 by the control valve 20, so that the reagent in the common channel 17 can enter the second channel 13. Similarly, it is only necessary to reasonably control the common channel 17 to be connected to the third channel 16 and the second channel 13 in sequence by the control valve 20, so that the transfer of fluid, such as a sample or a cleaning solution, between the third channel 16 and the second channel 13 can be achieved.

[0080] In some embodiments, the power module 30 is mainly used to provide power for the flow of fluids such as reagents, samples, and cleaning fluids in corresponding channels, as well as the mixing reaction of reagents and samples in the mixing chamber 15. Specifically, the power module 30 is a component that is removably attached to the mixing detection module 10. In the state where the power module 30 is attached to the mixing detection module 10, the power module 30 is connected to the common channel 17 to pressurize or depressurize the common channel 17 through the power module 30. In the state where the power module 30 is attached to the mixing detection module 10, the power module 30 is connected to the common channel 17, so that the power module 30 can include a gas device with the ability to change the gas pressure, such as a pump.

[0081] Thus, when it is necessary to transfer the reagent in a certain storage cavity 11 to the second channel 13, the control valve 20 is first used to control the common channel 17 to be connected to the first channel 12 of the corresponding storage cavity 11, and then the power module 30 decompresses the common channel 17 to form a negative pressure in the common channel 17, thereby attracting the reagent in the corresponding storage cavity 11 to the common channel 17 through the corresponding first channel 12; on this basis, the control valve 20 controls the common channel 17 to be connected to the second channel 13, and then the power module 30 pressurizes the common channel 17 to push the reagent in the common channel 17 to the second channel 13. Similarly, with the cooperation of the control valve 20 and the power module 30, the sample or cleaning fluid from the third channel 16 can be transferred to the second channel 13 in a substantially similar manner.

[0082] In order to more clearly and intuitively understand the microfluidic chip disclosed in the embodiment of the present invention, the operation method of the microfluidic chip is described below in conjunction with the process of analyzing a certain sample. For example, the sample can be urine.

[0083] Assume that the hybrid detection module 10 of the microfluidic chip has a plurality of third channels 16. Before using the microfluidic chip, one of the third channels 16 is connected to a sample source and another third channel 16 is connected to a cleaning liquid source, and then the sample analysis operation can be performed.

[0084] First, the sample to be analyzed is transferred to the mixing chamber 15 of the second channel 13. Specifically, under the control of the control valve 20, the third channel 16 connected to the sample source is connected to the common channel 17, and the power module 30 decompresses the common channel 17 so that the sample provided by the sample source flows through the corresponding third channel 16 and enters the common channel 17; then, under the control of the control valve 20, the common channel 17 is connected to the second channel 13, and the power module 30 pressurizes the common channel 17 so that the sample in the common channel 17 enters the second channel 13 and reaches the mixing chamber 15.

[0085] Secondly, the reagents used for analysis are transferred to the mixing chamber 15 of the second channel 13. Specifically, in a manner substantially the same as that required above, the control valve 20 controls the common channel 17 to communicate with the first channels 12 of different storage chambers 11, and cooperates with the power module 30 to attract the reagents in the corresponding storage chamber 11 to the common channel 17, and then the control valve 20 controls the common channel 17 to communicate with the second channel 13, and cooperates with the power module 30 to push the reagents in the common channel 17 to the second channel 13 and reach the mixing chamber 15.

[0086] After that, the reagent and sample in the mixing chamber 15 are fully mixed and reacted by the power module 30. Specifically, under the control of the control valve 20, the common channel 17 and the second channel 13 are kept connected. On this basis, the power module 30 repeatedly pressurizes or depressurizes the common channel 17 several times to repeatedly change the air pressure in the mixing chamber 15, so that the reagent and sample in the mixing chamber 15 are oscillated, and then the reagent and sample are fully mixed and reacted.

[0087] After the reagents and samples in the mixing chamber 15 complete the mixing reaction, the power module 30 continuously pressurizes the common channel 17 to push the mixed fluid formed by the mixing reaction of the reagents and samples in the mixing chamber 15 into the detection chamber 14 to complete the detection and analysis of the sample in the detection chamber 14.

[0088] After the sample detection and analysis is completed, if it is necessary to clean each channel, the mixed fluid in the detection chamber 14 is first drained as much as possible. Figure 7 The drainage port 141 shown in the figure can conveniently discharge the mixed fluid in the detection chamber 14. Specifically, after the detection and analysis of the sample in the detection chamber 14 is completed, the power module 30 can continue to pressurize the common channel 17 to push the mixed fluid in the detection chamber 14 out from the drainage port 141.

[0089] After draining the mixed fluid in the detection chamber 14 as much as possible, the cleaning liquid provided by the cleaning liquid source can be transferred in the channels to be cleaned (such as the common channel 17, the second channel 13, etc.) in turn, referring to the above-mentioned method of transferring the sample in each channel, so as to achieve cleaning of the corresponding channels.

[0090] In summary, it can be seen that the microfluidic chip disclosed in the embodiment of the present invention integrates a storage chamber 11 for providing reagents, a channel for the flow of fluids such as reagents and samples, a mixing chamber 15 for mixed reactions of fluids such as reagents and samples, and a detection chamber 14 for analyzing and detecting samples on a mixing detection module 10, and sets a control valve 20 and a power module 30 for realizing directional flow and mixed reaction of fluids in each channel as removable components relative to the mixing detection module 10. This not only improves the degree of integration of the microfluidic chip to reduce the volume of the microfluidic chip, but also facilitates the installation and replacement of the mixing detection module 10 as a consumable, thereby effectively improving the flexibility and convenience of the microfluidic chip when used.

[0091] For example, when the microfluidic chip is actually used, the control valve 20 and the power module 30 can be kept fixed as long-term components. On this basis, for the analysis and detection of different samples, only the mixed detection module 10 needs to be replaced without replacing the entire microfluidic chip.

[0092] At the same time, by setting the mixing chamber 15 for mixing reactions of reagents, samples and other fluids on the second channel 13, compared with the method of setting the mixing chamber 15 on the common channel 17, the residual amount of reagents, samples and other fluids in the common channel 17 can be effectively reduced, which is beneficial to reduce the burden of subsequent cleaning of each channel and improve the cleaning quality.

[0093] In addition, in the microfluidic chip disclosed in the embodiment of the present invention, the mixing reaction of the reagent and the sample in the mixing chamber 15 is achieved based on the power module 30 repeatedly changing the air pressure in the mixing chamber 15. Compared with the method of using an additional mixing device (such as an ultrasonic mixing device) to mix the reagent and the sample, it can effectively reduce the cost and further reduce the volume of the microfluidic chip.

[0094] The hybrid detection module 10 may be constructed in the following manner but is not limited thereto, so as to further optimize the structural design of the hybrid detection module 10 .

[0095] Combination Figure 2 and Figure 3As shown in the figure, the mixed detection module 10 may further include a storage box 18 and a substrate 19. The storage box 18 and the substrate 19 may be structures that overlap each other, the aforementioned multiple storage cavities 11 may be arranged on the storage box 18, and the first channel 12, the second channel 13, the third channel 16 and the common channel 17 corresponding to each storage cavity 11 may be arranged on the substrate 19.

[0096] Furthermore, the storage box 18 is detachably connected to the base plate 19. When the base plate 19 is connected to the storage box 18, the first channel 12 on the base plate 19 is connected to the corresponding storage chamber 11, so that the first channel 12 can receive the reagent from the corresponding storage chamber 11. At this time, the control valve 20 and the power module 30 can be removably attached to the side of the base plate 19 away from the storage box 18.

[0097] It is worth mentioning that by further dividing the mixed detection module 10 into a storage box 18 and a substrate 19, and setting the storage cavity 11 for storing reagents on the storage box 18, when the microfluidic chip is actually used, for the analysis and detection of the same sample, it is only necessary to replace the storage box 18 to replenish the reagents without replacing the entire mixed detection module 10, which further improves the flexibility and convenience of the microfluidic chip when using.

[0098] Reference Figure 3 As shown, the connection between the base plate 19 and the storage box 18 can be a snap connection, so as to facilitate the quick disassembly and assembly of the base plate 19 and the storage box 18. For example, a first male buckle 191 can be provided on the base plate 19, and a first female buckle 181 that cooperates with the first male buckle 191 can be provided on the storage box 18 to achieve the snap connection between the base plate 19 and the storage box 18.

[0099] In order to prevent leakage at the connection between the storage chamber 11 and the corresponding first channel 12 after the storage box 18 is connected to the base plate 19, Figure 3 , Figure 5 as well as Figure 8 As shown, a first connecting column 192 is provided on the side of the substrate 19 facing the storage box 18 . The first connecting column 192 corresponds one-to-one with the first channel 12 and extends toward the storage box 18 . The first end of the first channel 12 passes through the end of the first connecting column 192 .

[0100] The storage box 18 is provided with first grooves 182 corresponding to the storage cavities 11 on one side facing the base plate 19, and each first groove 182 is provided with an outlet 111 communicating with the corresponding storage cavity 11. In addition, the shape of a single first groove 182 is adapted to a single first connecting column 192, so that a single first connecting column 192 can be reliably plugged into the corresponding first groove 182, and a first sealing member 193 such as a sealing ring is provided between the outer wall of the first connecting column 192 and the inner wall of the first groove 182. Among them, the first connecting column 192 can be a truncated cone with a diameter gradually decreasing in the direction away from the base plate 19, so as to improve the reliability of the first connecting column 192 when it is plugged into the first groove 182.

[0101] Based on the above arrangement, when the storage box 18 and the base plate 19 are actually assembled, the first sealing member 193 is first sleeved on the outer wall of each first connecting column 192, and then, each first connecting column 192 on the base plate 19 is aligned one by one with the first groove 182 on the storage box 18, and the first connecting column 192 is inserted into the corresponding first groove 182, so that the first channel 12 is connected with the outlet 111 of the corresponding storage cavity 11 by means of the first end. In this process, the base plate 19 is snap-fitted with the first female buckle 181 on the storage box 18 by means of the first male buckle 191, so that the assembly of the base plate 19 and the storage box 18 can be realized. At this time, each first channel 12 is connected with the corresponding storage cavity 11, and under the action of the first sealing member 193 in each first groove 182, the reagent will not leak when it flows out from the outlet 111 of the storage cavity 11 to the corresponding first channel 12.

[0102] In order to facilitate the replenishment of corresponding reagents into each storage chamber 11 before using the microfluidic chip, such as Figure 4 As shown, each storage cavity 11 also has an inlet 112 for the reagent to enter the storage cavity 11, and a sealing portion (not shown in the figure) is provided at the inlet 112, which is configured to prevent the reagent in the storage cavity 11 from flowing out from the inlet 112 and maintain the air pressure balance between the inside of the storage cavity 11 and the external environment, so that when the first channel 12 of the storage cavity 11 is connected to the common channel 17 and the power module 30 decompresses the common channel 17, the reagent in the storage cavity 11 can flow out from the outlet 111 of the storage cavity 11 under the action of the power module 30. Among them, the sealing portion mentioned above can be a follow-up sealant that does not react with the reagent, for example, silicone oil, glycerin and other greases.

[0103] It is worth noting that by setting an inlet 112 for the storage chamber 11 and setting a follow-up sealant as a sealing part at the inlet 112, it is not only beneficial to replenish the corresponding reagent to the storage chamber 11 before using the microfluidic chip, but also can prevent the reagent in the storage chamber 11 from being contaminated due to contact with the external environment. At the same time, based on the fact that the follow-up sealant can maintain the air pressure balance between the inside of the storage chamber 11 and the external environment, in the subsequent use of the microfluidic chip, the reagent in the storage chamber 11 can flow out directly from the outlet 111 under the action of the power module 30 without puncturing the sealing part.

[0104] Further, such as Figure 4 As shown, the storage box 18 may include a first box body 183 and a first cover plate 184. The first box body 183 is detachably connected to the substrate 19, that is, the first female buckle 181 described above may be provided on the first box body 183. A plurality of storage cavities 11 are provided on a side of the first box body 183 away from the substrate 19, and the side of each storage cavity 11 away from the substrate 19 is in an open structure.

[0105] The first cover plate 184 is arranged close to the side of the first box body 183 away from the substrate 19 and forms a seal with the first box body 183, and the first cover plate 184 covers the openings of each storage cavity 11. For example, the first cover plate 184 and the first box body 183 can be connected together by means such as welding, bonding, etc. to ensure a reliable seal between the first cover plate 184 and the first box body 183 to prevent leakage of the storage cavity 11. At this time, the first groove 182 mentioned above is arranged on the side of the first box body 183 away from the first cover plate 184. It can be understood that by further dividing the storage box 18 into the first box body 183 and the first cover plate 184, it is convenient to process and manufacture multiple storage cavities 11.

[0106] Continue to refer to Figure 4 The outlet 111 and the inlet 112 on each storage cavity 11 can be arranged on two opposite sides of the storage cavity 11 to further optimize the structural design of the hybrid detection module 10 .

[0107] Further, such as Figure 5 As shown, the base plate 19 may further include a first plate body 194 and a second plate body 195 which are arranged to overlap each other. The first plate body 194 is detachably connected to the storage box 18, specifically the first box body 183 of the storage box 18, that is, the first male buckle 191 mentioned above may be arranged on the first plate body 194. At the same time, the first connecting column 192 mentioned above may be arranged on the side of the first plate body 194 away from the second plate body 195.

[0108] A plurality of first channels 12, second channels 13, third channels 16 and common channels 17 are provided on the side of the second plate 195 facing the first plate 194. The first plate 194 covers each channel and is sealed with the second plate 195. For example, the first plate 194 and the second plate 195 can be components formed together by bonding. At this time, the control valve 20 and the power module 30 can be removably attached to the side of the second plate 195 facing away from the first plate 194.

[0109] It can be understood that by further dividing the substrate 19 into a first plate body 194 and a second plate body 195 and arranging each channel on the second plate body 195 , it is facilitated to process and manufacture each channel.

[0110] In some embodiments, the hybrid detection module 10 may be further constructed in the manner described below, but is not limited thereto, so that the control valve 20 and the power module 30 can be attached to the hybrid detection module 10 and realize corresponding functions.

[0111] Specifically, Figure 2 or Fig. 9 As shown, the mixed detection module 10 is provided with a mounting surface 110. In this embodiment, the mounting surface 110 refers to the side of the substrate 19 facing away from the storage box 18, specifically refers to the side of the second plate 195 facing away from the first plate 194. The second ends of the first channels 12, the second channels 13, the third channels 16 and the common channel 17 all pass through the mounting surface 110, and refer to Figure 6 As shown, the second ends of the first channels 12, the second channels 13 and the third channels 16 are arranged on the same circumference with the second end of the common channel 17 as the center. The second end of the first channel 12 refers to the end of the first channel 12 away from the aforementioned storage cavity 11, the second end of the second channel 13 refers to the end away from the detection cavity 14, the second end of the third channel 16 refers to the end away from the external device (such as a sample source, a cleaning liquid source, etc.), and the second end of the common channel 17 refers to the end away from the power module 30 to be described in detail below.

[0112] The control valve 20 may be a rotary valve. Figures 9 to 11 As shown in the figure, the control valve 20 includes a rotating member 21, and the rotating member 21 has a sealing surface 211 and a flow guide channel 212. The sealing surface 211 of the rotating member 21 is sealed with the mounting surface 110 of the mixing detection module 10, the first end of the flow guide channel 212 penetrates the sealing surface 211 and is always connected with the second end of the common channel 17, and the second end of the flow guide channel 212 penetrates the sealing surface 211 and is located on the circumference mentioned above. The rotating member 21 is configured to be able to rotate around the center of the circumference, that is, the rotating member 21 can rotate around the second end of the common channel 17.

[0113] In this way, when the common channel 17 needs to be connected to different first channels 12, second channels 13 and third channels 16, it is only necessary to rotate the rotating member 21 so that the second end of the guide channel 212 is aligned with the second ends of different first channels 12, second channels 13 and third channels 16.

[0114] The control valve 20 may further include a positioning member 22, which is used to restrict the rotating member 21 to the mounting surface 110 of the mixing detection module 10, so that its sealing surface 211 is sealed with the mounting surface 110 of the mixing detection module 10, and allows the rotating member 21 to rotate around the center of the circle. That is, the positioning member 22 is immovable relative to the mixing detection module 10, and the rotating member 21 can rotate freely between the positioning member 22 and the mixing detection module 10 around the center of the circle.

[0115] Specifically, combined Figure 2 , Figures 9 to 11 As shown in the figure, the mounting surface 110 of the hybrid detection module 10 is provided with a clamping member 120, and the clamping member 120 defines a mounting cavity 121 enclosing the second ends of the first channels 12, the second channels 13, the third channels 16, and the common channel 17. The positioning member 22 includes a shell 221 and an elastic member 222, and the shell 221 defines a receiving cavity 223, and the shell 221 is snap-connected with the clamping member 120. For example, referring to Figure 2 and Fig.10 As shown, a second male buckle 122 can be provided on the outer wall of the snap-in component 120, and a second female buckle 224 adapted to the second male buckle 122 can be provided on the inner wall of the shell 221, so that the snap-in component 120 and the shell 221 can be snap-connected with the aid of the second male buckle 122 and the second female buckle 224.

[0116] like Fig.11 As shown, the rotating member 21 and the elastic member 222 are both arranged in the accommodating cavity 223 of the housing 221. The elastic member 222 is configured to provide elastic force to the rotating member 21 to force its sealing surface 211 to seal with the mounting surface 110 of the mixing detection module 10, and allow the rotating member 21 to rotate around the center of the circle. That is to say, when the control valve 20 and the mixing detection module 10 are assembled in place, the elastic member 222 can provide elastic force to the rotating member 21 to force the sealing surface 211 of the rotating member 21 to seal with the mounting surface 110 of the mixing detection module 10, thereby preventing leakage between the sealing surface 211 and the mounting surface 110, but the elastic force will not hinder the rotation of the rotating member 21. The elastic member 222 can be a spring, such as a disc spring, and one end of the spring is against the side of the rotating member 21 away from the mixing detection module 10, and the other end of the spring can be fixedly connected to the inner wall of the housing 221.

[0117] It is worth noting that the above-mentioned method is used to realize the connection and cooperation between the control valve 20 and the mixing detection module 10, which is conducive to quickly separating the control valve 20 from the mixing detection module 10 and realizing reliable sealing between the sealing surface 211 of the rotating part 21 and the mounting surface 110 of the mixing detection module 10.

[0118] Further, combined with Figures 9 to 11 As shown in the figure, the control valve 20 may further include a power source 23, which is used to provide power to the rotating member 21 to force it to rotate. Specifically, the power source 23 may include a connecting member 231, a transmission mechanism 232, and a motor 233. The connecting member 231 is coaxially arranged with the rotating member 21, for example, arranged on a side of the rotating member 21 away from the mixing detection module 10, and the connecting member 231 is transmission-connected with the motor 233 through the transmission mechanism 232, so that the motor 233 can drive the rotating member 21 to rotate by means of the transmission mechanism 232 and the connecting member 231.

[0119] Among them, a cross-shaped connecting groove can be set on the side of the rotating part 21 away from the installation surface 110 of the hybrid detection module 10, and the connecting part 231 can be connected to the rotating part 21 through the connecting groove to facilitate the quick connection between the rotating part 21 and the connecting part 231, and the connecting groove is provided with an anti-misinstallation structure. For example, the width of the cross-shaped connecting groove can be set to a different structure to prevent misinstallation.

[0120] The transmission mechanism 232 may include a transmission shaft 2321, a transmission gear set 2322, and a worm 2323. The transmission shaft 2321 is coaxially connected to the connecting member 231. The worm 2323 is transmission-connected to the output end of the motor 233, and the worm 2323 is transmission-connected to the transmission shaft 2321 through the transmission gear set 2322. In this way, the motor 233 can transmit power to the connecting member 231 by means of the worm 2323, the transmission gear set 2322, and the transmission shaft 2321, and then drive the rotating member 21 to rotate through the connecting member 231. The method of setting the worm 2323 as a transmission component at the output end of the motor 233 is helpful to optimize the structural design of the control valve 20. For example, the motor 233 can be set as a component parallel to the mounting surface 110 of the mixing detection module 10, so as to reduce the volume of the entire microfluidic chip in the direction of the connection between the mixing detection module 10 and the control valve 20.

[0121] In some embodiments, continue to refer to Figures 9 to 11The control valve 20 may further include a detection component 24, which is mainly used to detect the rotation angle of the rotating member 21, so as to improve the accuracy of the flow path switching according to the rotation angle of the rotating member 21. The detection component 24 may include a code disk 241 and a sensor 242 that cooperates with the code disk 241. The code disk 241 is coaxially arranged with the rotating member 21. For example, the code disk 241 may be arranged on the transmission shaft 2321 so that the code disk 241 can rotate coaxially with the rotating member 21. The sensor 242 is arranged beside the code disk 241 and is stationary relative to the code disk 241, so as to detect the rotation angle of the rotating member 21 through the cooperation between the sensor 242 and the code disk 241.

[0122] In some embodiments, in order to facilitate the attachment of the power module 30 to the hybrid detection module 10 and realize the conduction between the power module 30 and the common channel 17, Figure 12 to Figure 14 As shown, the power module 30 includes an adapter 31 , which is provided with a guide channel 311 and a second groove 312 corresponding to the guide channel 311 , and a first end of the guide channel 311 is located in the second groove 312 .

[0123] The power module 30 further includes a pump, an output end of the pump is connected to the second end of the guide channel 311 through a pipeline (not shown in the figure).

[0124] The mounting surface 110 of the hybrid detection module 10 is provided with a second connecting column 130 corresponding to the second groove 312, and the second connecting column 130 extends in a direction away from the mounting surface 110. The common channel 17 is provided with a connecting port 171 that passes through the end of the second connecting column 130. The shape of a single second groove 312 is adapted to a single second connecting column 130, so that the single second connecting column 130 can be reliably plugged into the corresponding second groove 312, and a second sealing member 140 such as a sealing ring is provided between the outer wall of the second connecting column 130 and the inner wall of the second groove 312. The second connecting column 130 can be a truncated cone with a diameter gradually decreasing in a direction away from the mounting surface 110, so as to improve the reliability of the second connecting column 130 when plugged into the second groove 312.

[0125] Based on the above arrangement, it is only necessary to align the second connecting column 130 at the mounting surface 110 of the hybrid detection module 10 with the corresponding second groove 312 on the adapter 31 and insert the second connecting column 130 into the corresponding second groove 312, so that the guide channel 311 can be connected to the common channel 17 by means of the corresponding connecting port 171. On this basis, the common channel 17 can be pressurized or depressurized only by operating the pump.

[0126] It is worth noting that adopting this method is conducive to simplifying the disassembly and assembly process between the power module 30 and the hybrid detection module 10 without disassembly and assembly of the pipeline connected to the pump.

[0127] Furthermore, based on the fact that during the actual use of the microfluidic chip, the amount of reagents required for sample analysis and detection often needs to be precisely controlled, and a large amount of cleaning liquid is often required to be transported when cleaning channels such as the common channel 17 and the second channel 13 in the mixed detection module 10. Therefore, Fig.14 As shown, there are two guide channels 311 on the adapter 31. In this case, the pump of the power module 30 may further include: Fig.12 The first pump 32 and the second pump 33 are shown, the output end of the first pump 32 is connected to the second end of one of the guide channels 311 through a pipeline, and the output end of the second pump 33 is connected to the second end of the other guide channel 311 through a pipeline.

[0128] The first pump 32 may be a pump with high fluid delivery accuracy at a small displacement, such as a syringe, and the second pump 33 may be a pump capable of delivering more fluid in a shorter time (i.e., having a larger displacement), such as a peristaltic pump. In addition, both the first pump 32 and the second pump 33 have a cut-off capability (i.e., the capability to prevent fluid backflow).

[0129] Thus, in combination with the above, during the sample analysis and detection stage, the first pump 32 can be started to pressurize or depressurize the common channel 17 to achieve the transfer of fluids such as reagents and samples, and the amount of fluids such as reagents and samples can be more accurately controlled. Correspondingly, when channel cleaning is required, the second pump 33 can be started to transfer the cleaning liquid in each channel to be cleaned, so that the amount of cleaning liquid entering each channel to be cleaned is sufficient to improve the cleaning quality.

[0130] In some embodiments, in combination Figures 2 to 3 , Figure 16 to Figure 17 As shown in the figure, the hybrid detection module 10 may further include a connection block 150 and a detection box 160. The connection block 150 may be arranged on the mounting surface 110 of the hybrid detection module 10, and a drainage channel 151 corresponding to the third channel 16 is provided on the connection block 150. The detection box 160 is detachably connected to the connection block 150, and the detection chamber 14 described above is defined in the detection box 160, and the detection chamber 14 is provided with a liquid inlet 142. The connection block 150 may be a component connected to the mounting surface 110 in a snap-fit ​​connection manner.

[0131] The first end of the second channel 13 penetrates the mounting surface 110 and communicates with the liquid inlet 142 of the detection chamber 14. The first end of each third channel 16 penetrates the mounting surface 110 and communicates with the first end of the corresponding drainage channel 151, and a connector can be provided at the second end of each drainage channel 151 to facilitate communication with external devices such as a sample source, a cleaning liquid source, etc. through the connector.

[0132] Thus, when adding a sample to the mixing chamber 15 or cleaning the channels on the mixed detection module 10, the sample provided by the external sample source or the cleaning liquid provided by the external cleaning liquid source will first flow through the corresponding drainage channel 151 and then enter the corresponding third channel 16. Accordingly, during the sample analysis and detection stage, the mixed fluid from the mixing chamber 15 will directly enter the detection chamber 14.

[0133] It is understandable that such a design helps to further optimize the structural design of the hybrid detection module 10, and the detection chamber 14 is arranged on the detachable detection box 160, which is conducive to replacing the detection chamber 14 with different detection substances according to the actual analysis and detection requirements. Among them, the connection between the detection box 160 and the connection block 150 can also be a snap connection.

[0134] Among them, Figure 7 and Fig.16 As shown, the first end of the second channel 13 and the detection chamber 14, and the first end of each third channel 16 and the first end of the corresponding drainage channel 151 can be connected by the aforementioned method of connecting columns, grooves and seals to prevent leakage.

[0135] Further, such as Fig.17 As shown, the detection box 160 may include a second box body 161 and a second cover plate 162. The detection chamber 14 is disposed on one side of the second box body 161 and is in an open structure. The second cover plate 162 is disposed close to the second box body 161 and forms a seal with the second box body 161, and the second cover plate 162 covers the opening of the detection chamber 14. It can be understood that by further dividing the detection box 160 into the second box body 161 and the second cover plate 162, it is convenient to process and manufacture the detection chamber 14.

[0136] Among them, combined Figure 7 or Fig.17 As shown, the detection chamber 14 can also be a chamber with a roughly L-shaped cross-section, and the liquid inlet 142 and the liquid outlet 141 are respectively located at the two ends of the detection chamber 14, so as to facilitate a more comprehensive analysis and detection of the sample in the mixed fluid from the mixing chamber 15, while facilitating the removal of air from the detection chamber 14 and reducing the residual amount of fluid in the detection chamber 14 when discharging the fluid in the detection chamber 14.

[0137] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A microfluidic chip, characterized in that: It includes a mixing detection module, a control valve and a power module; The hybrid detection module comprises: A plurality of storage chambers suitable for storing reagents, each of the storage chambers being equipped with a first channel; a second channel, wherein a mixing chamber is provided on the second channel; A detection chamber, connected to the mixing chamber; at least one third channel; Public access; The control valve is removably attached to the mixing detection module; wherein, when the control valve is attached to the mixing detection module, the control valve is configured to selectively allow the common channel to communicate with any one of the first channel, the second channel or the third channel; The power module is removably attached to the hybrid detection module; wherein, when the power module is attached to the hybrid detection module, the power module is connected to the common channel so as to pressurize or depressurize the common channel through the power module.

2. The microfluidic chip according to claim 1, characterized in that: The mixed detection module further comprises a storage box and a base plate, wherein the storage box and the base plate are arranged to overlap each other; The plurality of storage cavities are disposed in the storage box; the first channel, the second channel, the third channel and the common channel are disposed in the substrate; The storage box and the base plate are detachably connected; wherein, when the base plate is connected to the storage box, the first channel is connected to the corresponding storage cavity; The control valve and the power module are removably attached to a side of the base plate facing away from the storage box.

3. The microfluidic chip according to claim 2, characterized in that: A first connecting column is disposed on a side of the base plate facing the storage box, the first connecting column corresponds to the first channel one by one and extends toward the storage box, and a first end of the first channel passes through an end of the first connecting column; The storage box is provided with a first groove corresponding to the storage cavity one by one on one side facing the base plate, and the first groove is provided with an outlet communicating with the corresponding storage cavity; The first connecting column is inserted into the corresponding first groove, and a first sealing member is provided between the outer wall of the first connecting column and the inner wall of the first groove.

4. The microfluidic chip according to claim 1, characterized in that: The storage cavity has an inlet, and a sealing portion is provided at the inlet; The sealing portion is configured to prevent the reagent in the storage cavity from flowing out from the inlet and maintain the air pressure balance between the interior of the storage cavity and the external environment.

5. The microfluidic chip according to claim 1, characterized in that: The mixed detection module is provided with a mounting surface, the first channel, the second channel, the third channel and the second end of the common channel all pass through the mounting surface, and the second ends of the first channel, the second channel and the third channel are arranged on the same circumference with the second end of the common channel as the center; The control valve comprises a rotating member and a positioning member, wherein the rotating member has a sealing surface and a flow guide channel; the sealing surface is in sealing cooperation with the mounting surface, the first end of the flow guide channel penetrates the sealing surface and is always in communication with the second end of the common channel, and the second end of the flow guide channel penetrates the sealing surface and is located on the circumference; Wherein, the rotating member is configured to be able to rotate around the center of the circle; The positioning member is configured to restrict the rotating member to the mounting surface and allow the rotating member to rotate around the center of the circle.

6. The microfluidic chip according to claim 5, characterized in that: The mounting surface is provided with a clamping piece, and the clamping piece defines a mounting cavity enclosing the first channels, the second channels, the third channels and the second end of the common channel; The positioning member includes a shell and an elastic member; the shell defines a receiving cavity, and the shell is snap-connected to the clamping member; The rotating member and the elastic member are both arranged in the accommodating cavity; the elastic member is configured to provide the rotating member with an elastic force that forces the sealing surface to seal with the mounting surface and allows the rotating member to rotate.

7. The microfluidic chip according to claim 5, characterized in that: The control valve further comprises a power source, the power source being used to provide power to the rotating member to force it to rotate; The power source includes a connecting member, a transmission mechanism and a motor; the connecting member is coaxially arranged with the rotating member; The transmission mechanism includes a transmission shaft, a transmission gear set and a worm; the transmission shaft is coaxially connected to the connecting member, the worm is transmission-connected to the output end of the motor, and the worm is transmission-connected to the transmission shaft through the transmission gear set.

8. The microfluidic chip according to claim 5, characterized in that: The power module comprises an adapter and a pump, the adapter is provided with a guide channel and a second groove corresponding to the guide channel one by one, the first end of the guide channel is located in the second groove, and the second end of the guide channel is connected to the output end of the pump; The mounting surface is provided with second connecting columns corresponding to the second grooves one by one, the second connecting columns extend in a direction away from the mounting surface, and the common channel is provided with a connecting port penetrating through the ends of the second connecting columns; The second connecting column is inserted into the corresponding second groove, and a second sealing member is provided between the outer wall of the second connecting column and the inner wall of the second groove.

9. The microfluidic chip according to claim 8, characterized in that: There are two guide channels, and the pump includes a first pump and a second pump. The output end of the first pump is connected to the second end of one of the guide channels, and the output end of the second pump is connected to the second end of the other guide channel.

10. The microfluidic chip according to claim 5, characterized in that: The hybrid detection module also includes a connection block and a detection box; The connection block is arranged on the mounting surface, and a drainage channel corresponding to the third channel is arranged on the connection block; the detection box is detachably connected to the connection block, and the detection chamber is defined in the detection box, and the detection chamber is provided with a liquid inlet; The first end of the second channel passes through the mounting surface and is communicated with the liquid inlet; the first end of the third channel passes through the mounting surface and is communicated with the first end of the corresponding drainage channel.

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