DNA (deoxyribonucleic acid) synthesizer and liquid pumping and cleaning method

Abstract

The invention relates to the technical field of biology, and particularly discloses a DNA synthesizer and a liquid pumping and cleaning method. The reagent bottle is connected with a liquid feeding pipeline and a liquid injection needle, and a first valve body is arranged on the liquid feeding pipeline; the liquid inlet system comprises an alcohol supply assembly and a pure water supply assembly, the liquid outlet end of the alcohol supply assembly and the liquid outlet end of the pure water supply assembly are both communicated with the reagent bottle, and alcohol output by the alcohol supply assembly and pure water output by the pure water supply assembly can sequentially flow into the reagent bottle to clean the inner wall of the reagent bottle; the air outlet end of the air inlet system is communicated with the reagent bottle, and the air inlet system can introduce air into the reagent bottle, so that liquid in the reagent bottle is conveyed into the liquid conveying pipeline in a pressed mode and discharged from the liquid injection needle. The inner wall of the reagent bottle can be cleaned without opening the reagent bottle, so that the influence of frequent opening and closing of the bottle cap on the reagent bottle can be avoided, and the sealing performance of the reagent bottle can be ensured.

Description

Technical Field

[0001] The present application relates to the field of biotechnology, and in particular to a DNA synthesizer and a liquid cleaning method. Background Art

[0002] DNA synthesis technology is a key basic technology in synthetic biology. When the DNA synthesizer is working, the liquid injection module injects the reagents in the reagent bottle into the reaction chamber according to the synthesis sequence requirements to achieve synthesis. It is necessary to ensure that the reagent injection amount is accurate, and there is no leakage or pollution during the reaction; after a single synthesis is completed, the waste liquid in the reaction chamber needs to be completely discharged before starting the next round of synthesis.

[0003] After the DNA synthesizer is used, the inner wall of the reagent bottle needs to be cleaned to avoid crystallization on the inner wall of the reagent bottle, which will have an adverse effect on subsequent use. When cleaning the reagent bottle, it is necessary to first open the bottle cap of the reagent bottle, then fill the reagent bottle with cleaning liquid, clean the inner wall of the reagent bottle with the cleaning liquid, dissolve the substances adhering to the inner wall of the reagent bottle, then pour out the cleaning liquid in the reagent bottle, and then reinstall the bottle cap of the reagent bottle. Since a certain pressure of inert gas and an electromagnetic valve group are usually used in the DNA synthesizer to drive the movement of liquid reagents, frequent opening and closing of the reagent bottle is likely to affect the sealing of the reagent bottle, thereby affecting the discharge process of the reagent in the reagent bottle and affecting the normal use of the DNA synthesizer. Summary of the invention

[0004] In order to reduce the adverse effects of the cleaning process of the reagent bottle on the sealing of the reagent bottle, the present application provides a DNA synthesizer and a liquid cleaning method.

[0005] In a first aspect, the present application provides a DNA synthesizer, which adopts the following technical solution: A DNA synthesizer, comprising: The synthesizer body; A reagent bottle is arranged on one side of the synthesizer body, and a plurality of reagent bottles are arranged, each of which is connected to a liquid injection needle, and a liquid delivery pipe is connected between the reagent bottle and the liquid injection needle, and one end of the liquid delivery pipe away from the liquid injection needle is located at the bottom of the reagent bottle, and a first valve body is arranged on the liquid delivery pipe; The liquid inlet system includes an alcohol supply component and a pure water supply component, wherein the liquid outlet ends of the alcohol supply component and the pure water supply component are both connected to the reagent bottle, and the alcohol output by the alcohol supply component and the pure water output by the pure water supply component can flow into the reagent bottle in sequence to clean the inner wall of the reagent bottle; The air intake system includes a positive pressure air supply component, the air outlet end of which is connected to the reagent bottle, and the positive pressure air supply component can ventilate the reagent bottle so as to pressurize the reagent liquid, alcohol and pure water in the reagent bottle to the liquid delivery pipeline and discharge them from the injection needle; When the positive pressure gas supply component is used alone, the synthesizer performs a DNA synthesis reaction process; when the positive pressure gas supply component and the liquid inlet system are used in combination, the synthesizer performs a cleaning process.

[0006] By adopting the above technical scheme, alcohol can be first introduced into the reagent bottle, and the alcohol dissolves the substances attached to the inner wall of the reagent bottle, and then the alcohol is discharged; then pure water is introduced into the reagent bottle, and the pure water can dissolve the alcohol in the reagent bottle, and then the pure water is discharged; therefore, the substances attached to the inner wall of the reagent bottle can be washed away by two cleanings, so that impurities are not easily adhered to the inner wall of the reagent bottle to cause subsequent crystallization, thereby ensuring the cleanliness of the reagent bottle, and when the reagent bottle is used again, the reagent bottle is not easy to cause contamination to the introduced reagent; since the above cleaning process does not require the bottle cap of the reagent bottle to be removed, it helps to ensure the sealing of the reagent bottle, thereby ensuring the normal use of the reagent bottle; the alcohol and pure water discharged from the reagent bottle can be discharged through the liquid delivery pipe and the liquid injection needle, so the inner wall of the liquid delivery pipe and the liquid injection needle can also be cleaned, thereby avoiding blockage of the liquid delivery pipe and the liquid injection needle; the air intake system can introduce gas into the reagent bottle to discharge the liquid in the reagent bottle, which can not only discharge the alcohol and pure water used for cleaning, but also discharge the reagents used for DNA synthesis reaction, thereby helping to simplify the system.

[0007] Optionally, the alcohol supply assembly includes a pump body, a first pipeline, a second pipeline and an alcohol tank, a second valve body is provided on the first pipeline, the first pipeline is connected between the liquid inlet end of the pump body and the alcohol tank, the number of the second pipelines is the same as the number of reagent bottles, each of the second pipelines is connected between the liquid outlet end of the pump body and the corresponding reagent bottle, and a third valve body is provided on the second pipeline.

[0008] By adopting the above technical scheme, the pump body can transport the alcohol in the alcohol tank to the reagent bottle, thereby realizing alcohol cleaning of the reagent bottle; the second valve body is used to realize the switching of the cleaning state, when the second valve body is opened, alcohol cleaning can be performed, and when the second valve body is closed, pure water cleaning can be performed; the third valve body further enhances the flexibility of the system. When the required dose of alcohol is introduced into the smaller reagent bottle, the corresponding third valve body can be closed to stop the introduction of alcohol into this reagent bottle, while alcohol can continue to be introduced into the remaining reagent bottles. Therefore, even if each reagent bottle is of different sizes, the required dose of alcohol can be introduced into each reagent bottle, while the amount of alcohol can be reduced while ensuring the cleaning effect of the reagent bottle, thereby reducing waste.

[0009] Optionally, the positive pressure air supply assembly includes an air pump, a main air circuit and a branch air circuit, the main air circuit is connected between the air pump and the branch air circuit, the number of the branch air circuits is the same as the number of reagent bottles, and each of the branch air circuits is connected to a corresponding reagent bottle at one end away from the main air circuit. An exhaust pipe is provided on the branch air circuit, and a fourth valve body is provided on the exhaust pipe.

[0010] By adopting the above technical solution, after the air pump is started, gas can be introduced into the reagent bottle through the main air path and the branch air path, so that the gas pressure in the reagent bottle increases, and the liquid in the reagent bottle is squeezed into the liquid delivery pipe, thereby discharging the liquid in the reagent bottle.

[0011] Optionally, a fluid infusion component is provided on the outside of the reagent bottle, and the fluid infusion component includes a fluid infusion gas circuit, a fluid infusion pipeline, a reagent container and a fifth valve body. The fluid infusion gas circuit is connected between the branch gas circuit and the reagent container, the fluid infusion pipeline is connected between the reagent container and the reagent bottle, and the fifth valve body is provided on the fluid infusion gas circuit. When the fifth valve body is opened, the air pump can ventilate the reagent container to pressurize the reagent in the reagent container into the reagent bottle.

[0012] By adopting the above technical solution, reagents can be replenished without opening the reagent bottle, which effectively avoids the sealing problem caused by frequent opening of the reagent bottle, ensures that the reagents in the reagent bottle can be discharged stably, and improves the working efficiency and reliability of the DNA synthesizer.

[0013] Optionally, a blowing assembly is provided in the reagent bottle, and the blowing assembly includes an air supply pipe, a sixth valve body, a connecting ring, a connecting pipe and a driving component. The air supply pipe is connected to the air pump, the sixth valve body is arranged on the air supply pipe, the connecting ring is slidably arranged in the reagent bottle along the height direction of the reagent bottle, the interior of the connecting ring is provided with a cavity connected to the air supply pipe, the connecting pipe is arranged on the connecting ring, and there are multiple connecting pipes. The connecting pipe is connected to the cavity inside the connecting ring, and the opening of the connecting pipe faces the inner wall of the reagent bottle. The driving component is used to drive the connecting ring to slide reciprocatingly.

[0014] By adopting the above technical scheme, the air pump introduces gas into the connecting ring through the air supply pipe, and the gas in the connecting ring is discharged through the connecting tube, thereby blowing air to the inner wall of the reagent bottle, blowing the water droplets attached to the inner wall of the reagent bottle to the bottom of the reagent bottle, and then discharging the water under the action of positive pressure, thereby reducing the water droplets attached to the inner wall of the reagent bottle, avoiding the residual moisture in the reagent bottle from contaminating the reagent introduced next time or affecting the reagent concentration, which helps to ensure the cleaning effect; the water droplets attached to the inner wall of the reagent bottle can be blown off during the sliding of the connecting ring and the connecting tube, and after the blowing process is completed, the connecting ring and the connecting tube move up, and when the reagent, alcohol and pure water are subsequently added to the reagent bottle in turn, the liquid level of the liquid in the reagent bottle is always below the connecting ring and the connecting tube, so the connecting ring and the connecting tube are not easy to cause pollution to the liquid in the reagent bottle.

[0015] Optionally, the driving component includes an airbag and a spring, one end of the airbag is closed and fixedly connected to the connecting ring, the other end of the airbag is open and connected to the air supply pipe, the airbag is provided with an exhaust port, the spring is connected between the connecting ring and the inner wall of the reagent bottle, and the spring causes the connecting ring to have a tendency to slide toward the side close to the top of the reagent bottle.

[0016] By adopting the above technical solution, the air pump introduces gas into the airbag through the air supply pipe, which can make the airbag inflate and stretch, and the airbag causes the connecting ring to move downward; when the connecting ring moves to the bottom of the reagent bottle, the connecting pipe on the connecting ring is blown for a period of time, and then the sixth valve body is closed, and the spring causes the connecting ring to move up and reset. When the connecting ring moves up, it can compress the airbag, and the gas in the airbag is discharged through the exhaust port until the connecting ring moves up to the initial position; thereby, the connecting ring can be driven, and the driving of the connecting ring does not require the provision of an additional power source, so it is more energy-efficient.

[0017] Optionally, the bottom wall of the reagent bottle is spherical.

[0018] By adopting the above technical solution, the water blown to the bottom of the reagent bottle can fall downward along the spherical surface to the bottom end of the spherical surface, and then the water gathers at the bottom end. The water gathered at the bottom end covers the opening of the liquid delivery pipe, so that the positive pressure in the reagent bottle can more easily pressurize the water into the liquid delivery pipe, thereby discharging the residual water in the reagent bottle.

[0019] Optionally, the connecting tube is rotatably connected to a connecting ring, and an adjusting assembly is provided on the connecting ring, the adjusting assembly includes a motor, a connecting column, a connecting rope and a torsion spring, the motor is provided on the connecting ring, the connecting column and the output shaft of the motor are coaxially fixedly connected, the number of the connecting ropes is the same as the number of the connecting tubes, one end of the connecting rope is fixedly connected to the connecting column, and the other end of the connecting rope is fixedly connected to the connecting tube, so that when the connecting column rotates, the connecting tube can be driven to rotate through the connecting rope, and the torsion spring is connected between the connecting tube and the connecting ring.

[0020] By adopting the above technical solution, when the connecting ring moves to the bottom of the reagent bottle, the motor starts, causing the connecting column to rotate, so that the connecting rope arranged around the connecting column is unfolded, and the torsion spring can cause the connecting tube to rotate inward until the opening of the connecting tube faces the side away from the inner wall of the reagent bottle, thereby realizing the adjustment of the opening angle of the connecting tube; therefore, the connecting tube can better blow down the water droplets attached to the spherical surface at the bottom of the reagent bottle, so that more water gathers at the bottom end of the spherical surface, thereby facilitating the discharge of residual water.

[0021] Optionally, a support rod is provided in the reagent bottle, the connecting ring and the support rod are slidably connected, and a heating wire is provided in the support rod.

[0022] By adopting the above technical solution, the support rod can limit the sliding of the connecting ring, thereby helping to improve the stability of the connecting ring sliding process; the heating wire can heat the inside of the reagent bottle, thereby drying the small amount of moisture remaining inside the reagent bottle, further enhancing the cleaning effect; in addition, after the heating wire heats the air inside the reagent bottle, the heated air is discharged into the liquid delivery pipe, which can dry the moisture remaining on the inner wall of the liquid delivery pipe, thereby enhancing the cleaning effect of the inner wall of the liquid delivery pipe.

[0023] In a second aspect, the present application provides a liquid cleaning method, which adopts the following technical solution: A liquid cleaning method, using any of the above-mentioned DNA synthesizers, comprises the following steps: a. The alcohol supply component feeds alcohol into the reagent bottle, and the alcohol dissolves the substances attached to the inner wall of the reagent bottle; b. The positive pressure gas supply component introduces gas into the reagent bottle, pressurizes the alcohol in the reagent bottle to the liquid delivery pipe, and the alcohol in the liquid delivery pipe is discharged through the injection needle; c. The pure water supply component feeds pure water into the reagent bottle, and the pure water dissolves in the alcohol attached to the inner wall of the reagent bottle; d. The positive pressure gas supply component introduces gas into the reagent bottle, and pressurizes the pure water dissolved with alcohol in the reagent bottle to the liquid delivery pipeline, and the pure water in the liquid delivery pipeline is discharged through the injection needle; e. Add the reagent into the reagent bottle, and use the positive pressure gas supply component to introduce gas into the reagent bottle, pressurize the reagent in the reagent bottle into the liquid delivery pipeline, and the reagent in the liquid delivery pipeline is discharged through the injection needle.

[0024] By adopting the above technical solution, alcohol and pure water are introduced into the interior of the reagent bottle in sequence, so that the inner wall of the reagent bottle can be cleaned. Since the bottle cap of the reagent bottle does not need to be opened during the cleaning process, the influence of frequent opening of the bottle cap on the sealing of the reagent bottle can be reduced, which helps to ensure the normal use of the DNA synthesizer.

[0025] In summary, this application includes the following beneficial technical effects: 1. The inner wall of the reagent bottle can be cleaned without opening the reagent bottle, thereby avoiding the impact of frequent opening and closing of the bottle cap on the reagent bottle, which helps to ensure the sealing of the reagent bottle.

[0026] 2. When cleaning the reagent bottle, just add alcohol and pure water in turn. The cleaning process is relatively simple and helps to improve the cleaning efficiency.

[0027] 3. The air blowing component can blow off the water droplets attached to the inner wall of the reagent bottle, so that the water droplets fall to the bottom of the reagent bottle and then gather at the bottom of the spherical surface. The gathered water can cover the outside of the liquid delivery pipe. Therefore, the positive pressure in the reagent bottle can more easily squeeze the water at the bottom of the spherical surface into the liquid delivery pipe, thereby discharging the residual water in the reagent bottle, which helps to reduce the residual water in the reagent bottle and prevent the residual water from contaminating the subsequently loaded reagents or reducing the concentration of the reagents. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram of the overall structure of Example 1 of the present application; Figure 2 This is a schematic diagram of the structure of Example 1 of the present application without the refrigerator; Figure 3 is the pipeline arrangement diagram of Example 1 of the present application; Figure 4 is a pipeline arrangement diagram of Example 2 of the present application; Figure 5 This is a pipeline arrangement diagram used to display the fluid infusion component in Example 2 of the present application; Figure 6 is a cross-sectional view of the reagent bottle in Example 2 of the present application; Figure 7 This is a schematic diagram of the structure of the adjustment component used to illustrate the embodiment 2 of the present application; Figure 8 It is a cross-sectional view after the connecting ring in Example 2 of the present application slides to the bottom.

[0029] Figure numerals: 1. synthesizer body; 11. injection unit; 12. discharge unit; 13. transfer unit; 14. reaction unit; 15. waste liquid collection unit; 16. reagent refrigeration unit; 161. refrigerator; 162. reagent bottle; 17. electrical control unit; 2. injection needle; 21. liquid delivery pipeline; 211. first valve body; 3. alcohol supply assembly; 31. pump body; 32. first pipeline; 321. second valve body; 33. second pipeline; 331. third valve body; 332. first one-way valve; 34. alcohol tank; 4. pure water supply assembly; 41. third pipeline; 42. seventh valve body; 43. water tank; 5. positive pressure air supply assembly ; 51. air pump; 52. main air circuit; 521. filter pressure reducing valve; 522. pressure regulating proportional valve; 53. branch air circuit; 531. exhaust pipe; 5311. fourth valve body; 532. second one-way valve; 6. fluid replenishment assembly; 61. fluid replenishment air circuit; 62. fluid replenishment pipeline; 63. reagent container; 64. fifth valve body; 7. blowing assembly; 71. air supply pipe; 72. sixth valve body; 73. connecting ring; 731. mounting plate; 74. connecting pipe; 741. telescopic pipe; 75. driving component; 751. air bag; 752. spring; 8. adjustment assembly; 81. motor; 82. connecting column; 83. connecting rope; 84. torsion spring; 9. support rod. DETAILED DESCRIPTION

[0030] The following combination Figure 1-Figure 8 This application is described in further detail. Example 1

[0031] The present application embodiment discloses a DNA synthesizer. Figure 1 and Figure 2 The DNA synthesizer includes a synthesizer body 1, and a liquid injection unit 11 and a liquid discharge unit 12 are respectively arranged on both sides of the synthesizer body 1. A transfer unit 13 is arranged on the synthesizer body 1 below the liquid injection unit 11 and the liquid discharge unit 12, and a reaction unit 14 is arranged on the transfer unit 13; a reaction chamber is arranged in the reaction unit 14, and a DNA synthesis reaction can be carried out after adding reagents to the reaction chamber; the transfer unit 13 can make the reaction unit 14 move laterally between the liquid injection unit 11 and the liquid discharge unit 12. The liquid injection unit 11 is used to inject reagents into the reaction chamber, and the liquid discharge unit 12 is used to discharge waste liquid in the reaction chamber. A waste liquid collection unit 15, a reagent refrigeration unit 16 and a cleaning unit are also arranged on the outside of the synthesizer body 1. The waste liquid collection unit 15 can collect the discharged waste liquid. The reagent refrigeration unit 16 is used to store reaction reagents at low temperatures; the reagent refrigeration unit 16 includes a refrigeration box 161 and a reagent bottle 162 arranged in the refrigeration box 161. The height direction of the reagent bottle 162 is vertically arranged. There are multiple reagent bottles 162 with different sizes. The capacity of the reagent bottle 162 can be set according to needs.

[0032] Reference Figure 3 Each reagent bottle 162 is fixedly connected with a liquid delivery pipe 21, one end of which is inserted into the reagent bottle 162 and located at the bottom of the reagent bottle 162, and the other end of which is located outside the reagent bottle 162 and fixedly connected with a liquid injection needle 2. Therefore, the reagent in the reagent bottle 162 can be discharged through the liquid delivery pipe 21 and the liquid injection needle 2, so that the reagent is added to the reaction chamber, which is convenient for the subsequent DNA synthesis reaction. A first valve body 211 is provided on the liquid delivery pipe 21, and the first valve body 211 is a two-position three-way valve, which is used to control the opening and closing of the liquid delivery pipe 21; the first valve body 211 can also be other forms of solenoid valves, and any valve body that can control the opening and closing of the liquid delivery pipe 21 can be used.

[0033] The cleaning unit is used to clean the inner wall of the reagent bottle 162 and the inner wall of the liquid delivery pipe 21 to ensure the synthesis effect. The synthesizer body 1 is also provided with an electrical control unit 17, which includes a PLC control system and a touch screen interactive system, and can adjust equipment parameters and observe equipment operation through the touch screen.

[0034] When synthesizing DNA, the reaction unit 14 starts to heat so that the reaction chamber maintains a suitable reaction temperature. Then the synthesis sequence is imported and the synthesis program is started. The transfer unit 13 moves the reaction unit 14 to the bottom of the liquid-making unit 11, and the liquid-making unit 11 fills each reaction chamber with reagents as required. Then the reaction chamber is sealed, and the synthesis reaction of DNA begins in the reaction chamber. After the synthesis reaction is completed, the transfer unit 13 moves the reaction unit 14 to the bottom of the liquid-discharging unit 12, and the liquid-discharging unit 12 discharges the waste liquid in the reaction unit 14 into the waste liquid collection unit 15. After the liquid-discharging process is completed, the reaction unit 14 moves to the bottom of the liquid-making unit 11 again, and then the processes of liquid-making, reaction, and liquid-discharging are carried out in sequence, and the above cycle is repeated. After the sequence is fully synthesized, the reagent bottle 162 and the liquid delivery pipeline 21 are cleaned by the cleaning unit.

[0035] Reference Figure 3, the cleaning unit includes a liquid inlet system and an air inlet system. The liquid inlet system includes an alcohol supply component 3 and a pure water supply component 4. The alcohol supply component 3 includes a pump body 31, a first pipeline 32, a second pipeline 33 and an alcohol tank 34. The pump body 31 is a diaphragm liquid pump; the first pipeline 32 is fixedly connected between the liquid inlet end of the pump body 31 and the alcohol tank 34, and a second valve body 321 is provided on the first pipeline 32. The second valve body 321 is a two-position two-way valve for controlling the opening and closing of the first pipeline 32. The number of second pipelines 33 is the same as the number of reagent bottles 162, and each second pipeline 33 corresponds to a reagent bottle 162. The second pipeline 33 is fixedly connected between the liquid outlet end of the pump body 31 and the reagent bottle 162. A third valve body 331 is provided on the second pipeline 33. The third valve body 331 is a two-position two-way valve for controlling the opening and closing of the second pipeline 33. Therefore, when the second valve body 321 and the third valve body 331 are both opened, the pump body 31 can transport the alcohol in the alcohol tank 34 to the reagent bottle 162 along the first pipeline 32 and the second pipeline 33, dissolving the substances attached to the inner wall of the reagent bottle 162, thereby achieving alcohol cleaning of the reagent bottle 162. Among them, the second pipeline 33 is also provided with a first one-way valve 332, and the liquid in the first one-way valve 332 flows in the direction from the pump body 31 to the reagent bottle 162, thereby preventing the liquid from flowing in the opposite direction.

[0036] The pure water supply assembly 4 includes a third pipeline 41, a seventh valve body 42 and a water tank 43. The third pipeline 41 is fixedly connected between the liquid inlet end of the pump body 31 and the water tank 43, and pure water is stored in the water tank 43. The seventh valve body 42 is arranged on the third pipeline 41, and the seventh valve body 42 is a two-position two-way valve, which is used to control the opening and closing of the third pipeline 41. Therefore, when the seventh valve body 42 and the third valve body 331 are both opened, the pump body 31 can transport the pure water in the water tank 43 to the reagent bottle 162 along the third pipeline 41 and the second pipeline 33, dissolve the alcohol attached to the inner wall of the reagent bottle 162, thereby realizing the water washing of the reagent bottle 162.

[0037] The air intake system includes a positive pressure air supply assembly 5, which includes an air pump 51, a main air circuit 52 and a branch air circuit 53. The main air circuit 52 is fixedly connected between the air outlet end of the air pump 51 and the branch air circuit 53. The number of branch air circuits 53 is the same as the number of reagent bottles 162, and each branch air circuit 53 corresponds to a reagent bottle 162. One end of the branch air circuit 53 away from the main air circuit 52 is fixedly connected to the corresponding reagent bottle 162, so that when the air pump 51 is working, gas can be introduced into the reagent bottle 162 through the main air circuit 52 and the branch air circuit 53, so that the pressure in the reagent bottle 162 increases, thereby pressing the liquid in the reagent bottle 162 into the liquid delivery pipe 21, and then the liquid in the liquid delivery pipe 21 is discharged through the injection needle 2. When the liquid in the reagent bottle 162 is alcohol or pure water, the inner wall of the liquid delivery pipe 21 can be cleaned, thereby avoiding crystallization and blockage of the inner wall of the liquid delivery pipe 21; when the liquid in the reagent bottle 162 is a reagent, the reaction reagent can be transported.

[0038] Each branch gas path 53 is fixedly connected with an exhaust pipe 531, one end of the exhaust pipe 531 is connected with the branch gas path 53, and the other end of the exhaust pipe 531 is connected with the outside. The exhaust pipe 531 is provided with a fourth valve body 5311, which is a two-position two-way valve for controlling the opening and closing of the exhaust pipe 531. Therefore, when the fourth valve body 5311 is opened, the branch gas path 53 can be connected with the outside, so that when the liquid inlet system is conveying alcohol and pure water, the gas in the reagent bottle 162 can be discharged through the exhaust pipe 531, thereby helping to ensure the smooth progress of the alcohol and pure water conveying process.

[0039] Among them, the main gas circuit 52 is provided with a filter pressure reducing valve 521 and a pressure regulating proportional valve 522, so that the gas pressure output by the air pump 51 is relatively stable, which helps to ensure the discharge effect of the injection needle 2. The injection pressure of different types of liquids or media can also be controlled by pressure regulation, which is more adaptable. The branch gas circuit 53 is provided with a second one-way valve 532, and the flow direction of the gas in the second one-way valve 532 is to flow from the main gas circuit 52 into the reagent bottle 162, thereby preventing the gas from flowing in the opposite direction.

[0040] The implementation principle of Example 1 is as follows: after the DNA synthesis process is completed, the inner wall of the reagent bottle 162 and the liquid delivery pipe 21 needs to be flushed. In the initial state, all valve bodies are in a closed state. First, the second valve body 321, all the third valve bodies 331 and all the fourth valve bodies 5311 are opened, and the pump body 31 delivers the alcohol in the alcohol tank 34 to the reagent bottle 162; since the reagent bottles 162 are of different sizes, the alcohol in the smaller reagent bottle 162 first reaches the preset dose (two-thirds of the reagent bottle 162), and the third valve body 331 and the fourth valve body 5311 corresponding to the smaller reagent bottle 162 are closed, and the alcohol is stopped from being passed into the smaller reagent bottle 162; at this time, the remaining reagent bottles 162 are still passing alcohol, and the above operation is repeated. When the alcohol in all the reagent bottles 162 reaches the preset dose, the second valve body 321 is closed to stop delivering alcohol.

[0041] The amount of alcohol sent into the reagent bottle 162 is greater than the amount of the reagent originally installed in the reagent bottle 162, so the alcohol can dissolve the substances attached to the inner wall of the reagent bottle 162, and realize the alcohol cleaning of the reagent bottle 162. After the alcohol cleaning is completed, all the fourth valve bodies 5311 are opened, and the reagent bottle 162 is connected to the outside world, and then all the third valve bodies 331 are opened, so that the alcohol remaining in the second pipeline 33 can flow into the reagent bottle 162 by itself under the action of gravity, and the residual liquid in the pipeline is emptied. Then all the third valve bodies 331 and all the fourth valve bodies 5311 are closed, all the first valve bodies 211 are opened, and the air pump 51 is started. The gas discharged by the air pump 51 flows into the reagent bottle 162 after decompression and pressure regulation, so that the pressure in the reagent bottle 162 is increased, and then the alcohol in the reagent bottle 162 is squeezed into the liquid delivery pipeline 21, and the alcohol in the liquid delivery pipeline 21 is discharged through the liquid injection needle 2, so that the alcohol cleaning of the liquid delivery pipeline 21 and the liquid injection needle 2 can be realized.

[0042] Then open the seventh valve body 42, and then pass pure water into the reagent bottle 162 in the same way, and the pure water dissolves the alcohol in the reagent bottle 162. Then empty the residual liquid in the second pipeline 33 to prevent the subsequent reagent from mixing with the residual liquid and affecting the reagent concentration. Then pass gas into the reagent bottle 162 to send pure water into the liquid delivery pipe 21, and then make the pure water in the liquid delivery pipe 21 discharged through the liquid injection needle 2, thereby achieving pure water cleaning of the reagent bottle 162, the liquid delivery pipe 21 and the liquid injection needle 2, and further enhancing the cleaning effect. Example 2

[0043] Reference Figure 4 and Figure 5The difference between this embodiment and embodiment 1 is that a liquid infusion assembly 6 is provided on the outside of each reagent bottle 162 in this embodiment, and the liquid infusion assembly 6 includes a liquid infusion gas circuit 61, a liquid infusion pipeline 62, a reagent container 63 and a fifth valve body 64. The liquid infusion gas circuit 61 is fixedly connected between the branch gas circuit 53 and the reagent container 63, and the reagent container 63 is filled with a reagent solution; the liquid infusion pipeline 62 is fixedly connected between the reagent container 63 and the reagent bottle 162. The fifth valve body 64 is provided on the liquid infusion gas circuit 61, and the fifth valve body 64 is a two-position two-way valve, which is used to control the opening and closing of the liquid infusion gas circuit 61. Therefore, when the fifth valve body 64 is opened, the air pump 51 can introduce gas into the reagent container 63 through the main air circuit 52, the branch air circuit 53 and the liquid replenishment air circuit 61, so that the pressure in the reagent container 63 increases, and then the reagent in the reagent container 63 is pressed into the liquid replenishment pipeline 62, and the reagent in the liquid replenishment pipeline 62 then flows into the reagent bottle 162, thereby realizing the liquid replenishment of the reagent bottle 162. Therefore, the process of adding reagent to the reagent bottle 162 does not require opening the bottle cap of the reagent bottle 162, which helps to ensure the sealing of the reagent bottle 162.

[0044] During the process of adding the reagent into the reagent bottle 162 , the fourth valve body 5311 corresponding to the reagent bottle 162 is opened, thereby allowing the reagent to flow into the reagent bottle 162 smoothly.

[0045] Reference Figure 6 , the reagent bottle 162 is also provided with an air blowing assembly 7, which includes an air supply pipe 71, a sixth valve body 72, a connecting ring 73, a connecting pipe 74 and a driving component 75. The air supply pipe 71 is fixedly connected to the bottle mouth of the reagent bottle 162, one end of the air supply pipe 71 is located outside the reagent bottle 162 and is connected to the branch air path 53; the sixth valve body 72 is provided at one end of the air supply pipe 71 located outside the reagent bottle 162, and the sixth valve body 72 is a two-position two-way valve for controlling the opening and closing of the air supply pipe 71. The other end of the air supply pipe 71 is located inside the reagent bottle 162. The connecting ring 73 is a circular ring, and the connecting ring 73 is slidably arranged in the reagent bottle 162 along the vertical direction; a cavity is provided inside the connecting ring 73, and the cavity inside the connecting ring 73 is connected to one end of the air supply pipe 71 located inside the reagent bottle 162. The connecting tube 74 is disposed on the connecting ring 73, and a plurality of connecting tubes 74 are disposed and arranged in a circumferential array; the connecting tube 74 is communicated with the cavity inside the connecting ring 73, and the opening of the connecting tube 74 faces the inner wall of the reagent bottle 162. Therefore, after the sixth valve body 72 is opened, the gas in the branch gas path 53 can flow into the connecting ring 73 through the gas supply pipe 71, and then the gas in the connecting ring 73 is discharged through the connecting tube 74, thereby blowing air to the inner wall of the reagent bottle 162, blowing off the water droplets attached to the inner wall of the reagent bottle 162, thereby reducing the water droplets attached to the inner wall of the reagent bottle 162, and helping to enhance the cleaning effect of the reagent bottle 162.

[0046] The driving component 75 is used to drive the connecting ring 73 to slide back and forth, and the driving component 75 includes an airbag 751 and a spring 752. The connecting ring 73 is fixedly connected with a mounting plate 731; the airbag 751 is in an elongated shape, the bottom end of the airbag 751 is closed and fixedly connected to the top wall of the mounting plate 731, the top end of the airbag 751 is fixedly connected to the inner wall of the reagent bottle 162, and the top end of the airbag 751 is open and communicated with the air supply pipe 71. An exhaust port is provided on the airbag 751, and the exhaust port is small. Therefore, when gas is introduced into the airbag 751, the airbag 751 can be bulged by making the amount of gas entering the airbag 751 greater than the amount of gas discharged through the exhaust port. The spring 752 is fixedly connected between the top wall of the connecting ring 73 and the inner wall of the reagent bottle 162. Under normal conditions, the spring 752 is at its original length, the connecting ring 73 is located at the top side inside the reagent bottle 162, and the airbag 751 is in a compressed state. When the sixth valve body 72 is opened, the gas in the branch path 53 can flow into the airbag 751 through the air supply pipe 71, and the airbag 751 gradually swells and stretches, causing the connecting ring 73 to move downward and the spring 752 to be stretched; while moving downward, the connecting ring 73 can blow air toward the inner wall of the reagent bottle 162 through the connecting pipe 74, thereby blowing off the water droplets attached to the inner wall of the reagent bottle 162; when the connecting ring 73 moves to the bottom of the reagent bottle 162, the sixth valve body 72 is closed again, and the elastic force of the spring 752 can cause the connecting ring 73 to move up and reset. When the connecting ring 73 moves up, the airbag 751 is gradually compressed, and the gas in the airbag 751 can be discharged through the exhaust port until the connecting ring 73 moves to the top of the reagent bottle 162 and completes the reset.

[0047] Furthermore, the bottom wall of the reagent bottle 162 is spherical, so that the water droplets on the inner wall of the reagent bottle 162 can fall to the bottom of the reagent bottle 162 after being blown down, and then can slide down along the spherical surface. The water droplets can gather at the bottom of the spherical surface, and the bottom end of the liquid delivery pipe 21 is located at the bottom of the spherical surface, so that the water at the bottom of the reagent bottle 162 can more easily enter the liquid delivery pipe 21, and then under the action of positive pressure, the water in the liquid delivery pipe 21 is discharged through the injection needle 2.

[0048] It should be noted that a fork is provided at one end of the air supply pipe 71 located inside the reagent bottle 162, one end of the fork is fixedly connected to the connecting ring 73 and communicates with the cavity inside the connecting ring 73, and the other end of the fork is fixedly connected to the top of the airbag 751 and communicates with the inside of the airbag 751. The part where the fork on the air supply pipe 71 is connected to the connecting ring 73 is a hose, and this hose can be extended or contracted as the connecting ring 73 moves, so the sliding process of the connecting ring 73 is not easy to affect the connection between the air supply pipe 71 and the connecting ring 73, which helps to ensure the smooth sliding of the connecting ring 73.

[0049] Reference Figure 7 and Figure 8In order to make it easier for the water at the bottom of the reagent bottle 162 to slide down along the spherical surface, the opening direction of the connecting tube 74 can be adjusted. A connecting seat is fixedly connected to the connecting ring 73, and the connecting tube 74 is hinged on the connecting seat. A telescopic tube 741 is fixedly connected between the connecting tube 74 and the connecting ring 73. The telescopic tube 741 is a corrugated hose. The telescopic tube 741 keeps the connecting tube 74 and the cavity inside the connecting ring 73 in a connected state. An adjusting component 8 for adjusting the opening direction of the connecting tube 74 is provided on the connecting ring 73.

[0050] The adjustment assembly 8 includes a motor 81, a connecting column 82, a connecting rope 83 and a torsion spring 84. The motor 81 is fixedly connected to the mounting plate 731, and the output shaft of the motor 81 is arranged vertically; the output shaft of the motor 81 can be self-locking, that is, after the motor 81 stops running, the output shaft of the motor 81 cannot be rotated by the action of external force. The connecting column 82 is coaxially fixedly connected to the end of the output shaft of the motor 81. The connecting rope 83 is a flexible rope body, and the connecting rope 83 is not elastic; the number of the connecting ropes 83 is the same as the number of the connecting tubes 74, and each connecting rope 83 corresponds to a connecting tube 74. One end of the connecting rope 83 is fixedly wound around the connecting column 82, and the other end of the connecting rope 83 is fixedly connected to the outer wall of the connecting tube 74. The number of torsion springs 84 is adapted to the number of the connecting tubes 74, and each torsion spring 84 corresponds to a connecting tube 74, and the torsion spring 84 is fixedly connected between the connecting tube 74 and the connecting seat. Under normal conditions, the torsion spring 84 is in a twisted state, so that the opening of the connecting tube 74 faces the inner wall of the reagent bottle 162, and the connecting rope 83 is in a tensioned and straight state.

[0051] After the connecting ring 73 moves to the bottom of the reagent bottle 162, the motor 81 is started, so that the connecting column 82 rotates, so that the connecting rope 83 wound on the connecting column 82 is unfolded, and the torsion spring 84 rotates the connecting tube 74 inward until the opening of the connecting tube 74 faces the side away from the inner wall of the reagent bottle 162. At this time, the connecting tube 74 blows air outward, which can better blow down the water droplets on the bottom wall of the reagent bottle 162, so that the water droplets attached to the bottom wall of the reagent bottle 162 can slide down to the bottom end of the spherical surface more easily.

[0052] In other embodiments, the bottom wall of the reagent bottle 162 may also be configured to be conical.

[0053] A vertical support rod 9 is also provided in the reagent bottle 162, and two support rods 9 are provided and arranged in parallel at intervals. A guide hole is provided on the mounting plate 731, and the support rod 9 is slidably inserted into the guide hole, so that the support rod 9 can limit the sliding of the connecting ring 73, thereby improving the stability of the sliding process of the connecting ring 73. A heating wire is also provided in the support rod 9, so that the heating wire can heat the inside of the reagent bottle 162 after being energized, thereby drying a small amount of water remaining at the bottom of the reagent bottle 162, and enhancing the cleaning effect of the reagent bottle 162.

[0054] The implementation principle of Example 2 is as follows: first, the inner wall of the reagent bottle 162 is cleaned with alcohol, and then the alcohol is discharged; then, the inner wall of the reagent bottle 162 is cleaned with pure water, and then the pure water is discharged. After the cleaning process is completed, the sixth valve body 72 is opened to allow gas to pass into the air supply pipe 71, and a part of the gas in the air supply pipe 71 flows into the air bag 751, so that the air bag 751 swells and stretches, and the stretched air bag 751 drives the connecting ring 73 to move downward; another part of the gas in the air supply pipe 71 flows into the connecting ring 73, and the gas in the connecting ring 73 is then discharged through the connecting pipe 74, blowing the inner wall of the reagent bottle 162, blowing off the water droplets attached to the inner wall of the reagent bottle 162, so that the water droplets fall to the bottom of the reagent bottle 162.

[0055] When the connecting ring 73 slides to the bottom of the reagent bottle 162, the motor 81 is started, so that the connecting column 82 rotates, the connecting rope 83 arranged around the connecting column 82 is unfolded, and the torsion spring 84 makes the connecting pipe 74 rotate inward until the opening of the connecting pipe 74 faces the side away from the inner wall of the reagent bottle 162, so that the connecting pipe 74 changes the blowing direction, thereby making it easier to blow the water droplets at the bottom of the reagent bottle 162 along the spherical surface to the bottom. At the same time, the inside of the reagent bottle 162 is still constantly passing gas, so the positive pressure can pressurize the water at the bottom of the reagent bottle 162 to the liquid delivery pipeline 21, and then discharge it through the liquid injection needle 2, thereby reducing the residual water inside the reagent bottle 162 and ensuring the cleaning effect.

[0056] During the blowing process, the heating wire in the support rod 9 is energized and heated, and the interior of the reagent bottle 162 is heated, thereby further drying the residual water in the reagent bottle 162, which helps to further enhance the cleaning effect. The heated gas is discharged into the liquid delivery pipe 21, so that the water attached to the inner wall of the liquid delivery pipe 21 can also be dried.

[0057] After the blowing process is completed, the motor 81 causes the connecting column 82 to rotate in the opposite direction, so that the connecting rope 83 on the connecting column 82 is rolled up, and the connecting pipe 74 is pulled to rotate outward by the connecting rope 83 until the opening of the connecting pipe 74 faces the inner wall of the reagent bottle 162. Then the sixth valve body 72 is closed, and the ventilation to the air bag 751 is stopped. The spring 752 causes the connecting ring 73 to move up and reset, so that the air bag 751 is compressed, and the gas in the air bag 751 is discharged through the exhaust port until the connecting ring 73 moves up to the initial position. When the reagent, alcohol and pure water are subsequently introduced into the reagent bottle 162, the liquid level of the liquid is always located below the connecting ring 73, so that the connecting ring 73 and the connecting pipe 74 do not directly contact the liquid, and it is not easy to cause pollution to the liquid in the reagent bottle 162.

[0058] This embodiment also discloses a liquid cleaning method, comprising the following steps: Step a. Open the second valve body 321 , all the third valve bodies 331 and all the fourth valve bodies 5311 , and the pump body 31 delivers the alcohol in the alcohol tank 34 to the reagent bottle 162 , and cleans the inner wall of the reagent bottle 162 with alcohol.

[0059] Step b. When the alcohol in the smaller reagent bottle 162 reaches a preset dose, the corresponding third valve body 331 and fourth valve body 5311 are closed. Meanwhile, the remaining reagent bottles 162 are still in a state of being filled with alcohol.

[0060] Step c. When the alcohol in all reagent bottles 162 reaches the preset dose, open all third valve bodies 331 and fourth valve bodies 5311 to discharge the residual liquid in the second pipeline 33 into the reagent bottle 162; then close the second valve body 321 and all third valve bodies 331 and fourth valve bodies 5311.

[0061] Step d. Open all the first valve bodies 211, start the air pump 51, and allow the air pump 51 to pass gas into the reagent bottle 162 to pressurize the alcohol in the reagent bottle 162 into the liquid delivery pipe 21. The alcohol in the liquid delivery pipe 21 is then discharged through the liquid injection needle 2 to achieve cleaning of the liquid delivery pipe 21 and the liquid injection needle 2; after cleaning, close the first valve body 211.

[0062] Step e. Open the seventh valve body 42 , all the third valve bodies 331 and all the fourth valve bodies 5311 , and the pump body 31 delivers the pure water in the water tank 43 to the reagent bottle 162 , and cleans the inner wall of the reagent bottle 162 with pure water.

[0063] Step f. When the pure water in the smaller reagent bottle 162 reaches a preset dose, the corresponding third valve body 331 and the fourth valve body 5311 are closed. Meanwhile, the remaining reagent bottles 162 are still in a state of being supplied with pure water.

[0064] Step g. When the pure water in all reagent bottles 162 reaches the preset dose, open all third valve bodies 331 and fourth valve bodies 5311 to discharge the residual liquid in the second pipeline 33 into the reagent bottle 162; then close the seventh valve body 42 and all third valve bodies 331 and fourth valve bodies 5311.

[0065] Step h. Open all the first valve bodies 211, start the air pump 51, and allow the air pump 51 to introduce gas into the reagent bottle 162 to pressurize the pure water in the reagent bottle 162 into the liquid delivery pipe 21. The pure water in the liquid delivery pipe 21 is then discharged through the liquid injection needle 2 to achieve re-cleaning of the liquid delivery pipe 21 and the liquid injection needle 2; after cleaning, close the first valve body 211.

[0066] Step i. Open the sixth valve body 72, and part of the gas discharged from the air pump 51 flows into the reagent bottle 162, and the other part flows into the air supply pipe 71; part of the gas in the air supply pipe 71 flows into the airbag 751, causing the airbag 751 to swell and stretch, and the connecting ring 73 moves downward; another part of the gas in the air supply pipe 71 flows into the connecting ring 73, and the gas in the connecting ring 73 is then discharged through the connecting pipe 74, thereby blowing the inner wall of the reagent bottle 162, and blowing the water droplets attached to the inner wall of the reagent bottle 162 to the bottom of the reagent bottle 162.

[0067] Step j. After the connecting ring 73 slides to the bottom of the reagent bottle 162, the motor 81 drives the connecting column 82 to rotate, and the connecting rope 83 arranged around the connecting column 82 unfolds, and the torsion spring 84 causes the connecting tube 74 to rotate inward, changing the blowing direction of the connecting tube 74, and blowing the water droplets on the spherical surface at the bottom of the reagent bottle 162 downward to the bottom end of the spherical surface. The positive pressure of the gas then presses the water droplets at the bottom end into the liquid delivery pipe 21, and then discharged through the liquid injection needle 2.

[0068] Step k. The heating wire is energized to heat the interior of the reagent bottle 162 to dry the residual water in the reagent bottle 162, thereby further reducing the residual water.

[0069] Step 1. After the blowing is completed, the motor 81 drives the connecting column 82 to rotate in the opposite direction, and the connecting rope 83 wound around the connecting column 82 is wound up, and the connecting rope 83 causes the connecting tube 74 to rotate outward; the sixth valve body 72 is closed, and the spring 752 causes the connecting ring 73 to move up and reset, and the gas in the airbag 751 is discharged through the exhaust port, and the airbag 751 returns to the compressed state.

[0070] The above are optional embodiments of the present application, and are not intended to limit the protection scope of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A DNA synthesizer, characterized in that: include: Synthesizer body (1); A reagent bottle (162) is arranged on one side of the synthesizer body (1), and a plurality of reagent bottles (162) are arranged, each of the reagent bottles (162) is connected to a liquid injection needle (2), a liquid delivery pipe (21) is connected between the reagent bottle (162) and the liquid injection needle (2), an end of the liquid delivery pipe (21) away from the liquid injection needle (2) is located at the bottom of the reagent bottle (162), and a first valve body (211) is arranged on the liquid delivery pipe (21); The liquid inlet system comprises an alcohol supply component (3) and a pure water supply component (4), wherein the liquid outlet ends of the alcohol supply component (3) and the pure water supply component (4) are both connected to the reagent bottle (162), and the alcohol output by the alcohol supply component (3) and the pure water output by the pure water supply component (4) can flow into the reagent bottle (162) in sequence to clean the inner wall of the reagent bottle (162); The air intake system comprises a positive pressure air supply component (5), wherein the air outlet end of the positive pressure air supply component (5) is connected to the reagent bottle (162), and the positive pressure air supply component (5) can ventilate the reagent bottle (162) so as to pressure-feed the reagent liquid, alcohol and pure water in the reagent bottle (162) to the liquid delivery pipe (21) and discharge them from the liquid injection needle (2); Wherein, when the positive pressure gas supply component (5) is used alone, the synthesizer performs a DNA synthesis reaction process; when the positive pressure gas supply component (5) and the liquid inlet system are used in combination, the synthesizer performs a cleaning process; The reagent bottle (162) is provided with an air blowing assembly (7), the air blowing assembly (7) comprising an air supply pipe (71), a sixth valve body (72), a connecting ring (73), a connecting pipe (74) and a driving component (75), the air supply pipe (71) being connected to the air pump (51), the sixth valve body (72) being provided on the air supply pipe (71), the connecting ring (73) being slidably provided in the reagent bottle (162) along the height direction of the reagent bottle (162), the connecting ring (73) being provided with a cavity communicating with the air supply pipe (71), the connecting pipe (74) being provided on the connecting ring (73), a plurality of connecting pipes (74) being provided, the connecting pipes (74) being communicated with the cavity inside the connecting ring (73), the opening of the connecting pipe (74) facing the inner wall of the reagent bottle (162), and the driving component (75) being used for driving the connecting ring (73) to slide back and forth.

2. A DNA synthesizer according to claim 1, characterized in that: The alcohol supply assembly (3) comprises a pump body (31), a first pipeline (32), a second pipeline (33) and an alcohol tank (34); a second valve body (321) is provided on the first pipeline (32); the first pipeline (32) is connected between a liquid inlet end of the pump body (31) and the alcohol tank (34); the number of the second pipelines (33) is the same as the number of the reagent bottles (162); each of the second pipelines (33) is connected between a liquid outlet end of the pump body (31) and a corresponding reagent bottle (162); and a third valve body (331) is provided on the second pipeline (33).

3. A DNA synthesizer according to claim 1, characterized in that: The positive pressure air supply assembly (5) comprises an air pump (51), a main air circuit (52) and a branch air circuit (53); the main air circuit (52) is connected between the air pump (51) and the branch air circuit (53); the number of the branch air circuits (53) is the same as the number of the reagent bottles (162); one end of each branch air circuit (53) away from the main air circuit (52) is connected to a corresponding reagent bottle (162); an exhaust pipe (531) is provided on the branch air circuit (53); and a fourth valve body (5311) is provided on the exhaust pipe (531).

4. A DNA synthesizer according to claim 1, characterized in that: A liquid infusion component (6) is arranged on the outside of the reagent bottle (162), and the liquid infusion component (6) includes a liquid infusion gas circuit (61), a liquid infusion pipeline (62), a reagent container (63) and a fifth valve body (64). The liquid infusion gas circuit (61) is connected between the branch gas circuit (53) and the reagent container (63), the liquid infusion pipeline (62) is connected between the reagent container (63) and the reagent bottle (162), and the fifth valve body (64) is arranged on the liquid infusion gas circuit (61). When the fifth valve body (64) is opened, the air pump (51) can ventilate the reagent container (63) so as to pressurize the reagent in the reagent container (63) into the reagent bottle (162).

5. A DNA synthesizer according to claim 1, characterized in that: The driving component (75) comprises an airbag (751) and a spring (752); one end of the airbag (751) is closed and fixedly connected to the connecting ring (73); the other end of the airbag (751) is open and communicates with the air supply pipe (71); an exhaust port is provided on the airbag (751), so that the amount of gas entering the airbag (751) is greater than the amount of gas discharged through the exhaust port, and the airbag (751) can swell; the spring (752) is connected between the connecting ring (73) and the inner wall of the reagent bottle (162); the spring (752) causes the connecting ring (73) to have a tendency to slide toward a side close to the top of the reagent bottle (162).

6. A DNA synthesizer according to claim 1, characterized in that: The bottom wall of the reagent bottle (162) is spherical.

7. A DNA synthesizer according to claim 1, characterized in that: The connecting tube (74) is rotatably connected to the connecting ring (73). The connecting ring (73) is provided with an adjustment assembly (8). The adjustment assembly (8) comprises a motor (81), a connecting column (82), a connecting rope (83) and a torsion spring (84). The connecting ring (73) is fixedly connected to a mounting plate (731). The motor (81) is arranged on the mounting plate (731). The connecting column (82) and the output shaft of the motor (81) are coaxially fixedly connected. The number of the connecting ropes (83) is the same as the number of the connecting tubes (74). One end of the connecting rope (83) is fixedly connected to the connecting column (82), and the other end of the connecting rope (83) is fixedly connected to the connecting tube (74), so that when the connecting column (82) rotates, the connecting tube (74) can be driven to rotate through the connecting rope (83). The torsion spring (84) is connected between the connecting tube (74) and the connecting ring (73).

8. A DNA synthesizer according to claim 1, characterized in that: A support rod (9) is provided in the reagent bottle (162), the connecting ring (73) and the support rod (9) are slidably connected, and a heating wire is provided in the support rod (9).

9. A method for cleaning a DNA synthesizer using any one of claims 1 to 8, characterized in that: The liquid cleaning method comprises the following steps: a. The alcohol supply component (3) supplies alcohol into the reagent bottle (162), and the alcohol dissolves the substance attached to the inner wall of the reagent bottle (162); b. The positive pressure gas supply component (5) introduces gas into the reagent bottle (162) to pressurize the alcohol in the reagent bottle (162) to the liquid delivery pipe (21), and the alcohol in the liquid delivery pipe (21) is discharged through the injection needle (2); c. The pure water supply component (4) supplies pure water into the reagent bottle (162), and the pure water dissolves in the alcohol attached to the inner wall of the reagent bottle (162); d. The positive pressure gas supply component (5) introduces gas into the reagent bottle (162), and the pure water dissolved with alcohol in the reagent bottle (162) is pressure-transmitted into the liquid delivery pipe (21), and the pure water in the liquid delivery pipe (21) is discharged through the liquid injection needle (2); e. Add the reagent into the reagent bottle (162), and the positive pressure gas supply component (5) introduces gas into the reagent bottle (162), so as to pressurize the reagent in the reagent bottle (162) into the liquid delivery pipe (21), and the reagent in the liquid delivery pipe (21) is discharged through the liquid injection needle (2).

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