High-pressure immunohistochemical system and method

Abstract

The invention relates to a biological reaction device, in particular to a high-pressure immunohistochemical system and method. The invention provides a high-pressure immunohistochemical system. The high-pressure immunohistochemical system comprises a thermal repair reaction device, an incubation device and a turnover mechanism, the thermal repair reaction device, the incubation device and the turnover mechanism are arranged; sample carriers can be accommodated in the thermal repair reaction device and the incubation device; and the overturning mechanism is used for overturning or / and moving the sample carrier. According to the method disclosed by the invention, the sample carrier is accurately positioned in different operation stages through the cooperation of the thermal repair reaction device, the incubation device and the turnover mechanism. And the overturning mechanism can ensure that the sample is covered in the liquid adding and incubation links, so that the loss and evaporation of the reagent are reduced.

Description

Technical Field

[0001] The invention relates to a bioreaction device, in particular to a high-pressure immunohistochemistry system and method. Background Art

[0002] In the field of biotechnology, immunohistochemistry is a commonly used method to study the expression of specific proteins in cell or tissue samples. This technology has a wide range of applications in medical diagnosis, drug development, and basic scientific research.

[0003] Immunohistochemistry is the application of the basic principle of immunology - antigen-antibody reaction, that is, the principle of specific binding of antigen and antibody. It uses chemical reaction to make the color developer (fluorescein, enzyme, metal ion, isotope) labeled with antibody develop color to determine the antigen (peptide and protein) in tissue cells, and conduct localization, qualitative and relative quantitative research on it. This is called immunohistochemistry or immunocytochemistry.

[0004] In the prior art immunohistochemistry system, when used in the process of adding reagents and incubating, the sample carrier (such as a glass slide) is usually arranged with the front side facing upward, which has the following defects: 1. Reagent loss and evaporation: Since the sample surface is exposed to the air, the reagent is easy to evaporate or lose from the glass slide, causing the sample tissue to be directly exposed to the air and dry, affecting the experimental results.

[0005] 2. Reagents react with non-antigenic substances: Exposed samples easily react with substances in the air, resulting in an increase in nonspecific reactions and affecting detection accuracy. Summary of the invention

[0006] 1. Technical issues to be resolved In view of the above-mentioned shortcomings and deficiencies of the prior art, the present invention provides a high-pressure immunohistochemistry system, in which the setting of the flipping mechanism can make the sample carrier inverted on the container containing reagents, thereby preventing the loss and evaporation of reagents, reducing nonspecific reactions and a more efficient incubation process.

[0007] Accordingly, the present invention also provides a high-pressure immunohistochemistry method, in which the sample carrier is covered with reagents and incubated, which can improve specificity.

[0008] (II) Technical solution In order to achieve the above object, the main technical solutions adopted by the present invention include: In a first aspect, the present invention provides a high-pressure immunohistochemistry system, which includes a thermal repair reaction device, an incubation device, and a flipping mechanism; The thermal repair reaction device and the incubation device can both accommodate a sample carrier; The flipping mechanism is used for flipping and / or moving the sample carrier.

[0009] The present invention realizes accurate positioning of the sample carrier at different operation stages through the cooperation of the thermal repair reaction device, the incubation device and the flip mechanism. The flip mechanism can ensure that the sample is covered during the steps of adding liquid and incubation, thereby reducing the loss and evaporation of reagents.

[0010] Optionally, the thermal repair reaction device comprises: a reactor body, a sample carrier placement rack, a reaction drive mechanism, and a heating element; In the present invention, a heating element is provided in the reactor body, which can provide a stable temperature environment for the sample to perform dewaxing and thermal repair treatment. The reaction drive mechanism can drive the reactor cover to move, making the loading and unloading of the sample carrier more convenient.

[0011] The reactor body comprises a reactor wall and a reactor cover, wherein the reactor wall and the reactor cover form a receiving cavity capable of receiving a sample carrier; The reactor cover is connected to a sample carrier placement rack on one side close to the accommodating cavity; The reactor cover is driven by a reaction drive mechanism to move closer to or away from the reactor wall; The heating element is disposed on the reactor body.

[0012] Optionally, the thermal repair reaction device further comprises a temperature sensor and a thermal repair control unit, and a temperature probe of the temperature sensor is arranged on the reactor body; The thermal repair control unit includes a temperature control module and a drive module. The temperature control module is electrically connected to the temperature sensor and is used to control the heating element based on the temperature reading of the temperature sensor; the drive module is electrically connected to the reaction drive mechanism and is used to drive the movement of the reactor cover.

[0013] In this scheme, the temperature sensor is connected to the thermal repair control unit, and the output of the heating element is controlled through temperature feedback to ensure that the temperature in the reaction device is always within the set range, thereby ensuring the accuracy and repeatability of the experiment.

[0014] Optionally, the sample carrier placement rack is provided with a limiting member for limiting the vertical placement of the sample carrier.

[0015] In this solution, the limiter ensures that the sample carrier can be placed vertically during loading, unloading and flipping, avoiding tilting and shifting, and improving the accuracy of incubation and processing.

[0016] The stoppers keep the sample carrier in an upright position, preventing it from tilting or shifting during operation, ensuring a stable reaction during heating or incubation. Placing the sample carrier vertically saves space and enables the system to accommodate more samples in a smaller volume, thereby improving operational efficiency. Because the samples are placed vertically and densely arranged, higher pressures can be achieved in a smaller space, improving the effect of the thermal repair treatment within the reactor body. Uniform heating: The vertical arrangement ensures that each sample carrier is evenly exposed to the heat of the heating element, thereby achieving uniform dewaxing and thermal repair effects. In summary, the design of the stoppers not only ensures that the sample carrier remains upright during operation, but also improves the space utilization of the system, achieving more efficient and stable immunohistochemistry processing.

[0017] Optionally, the flipping mechanism includes a rotating arm, a flipping motor and a first suction member. The end of the rotating arm is provided with a first suction piece for adsorbing the sample carrier, and the first suction piece is used to adsorb and put down the sample carrier. The output shaft of the flip motor is connected to the rotating shaft of the rotating arm to drive the rotation of the rotating arm; In this solution, the rotating arm can rotate to absorb or put down the sample carrier through the drive of the flipping motor, thereby realizing the flipping or moving operation and ensuring the correct positioning of the sample carrier between different devices.

[0018] Optionally, it includes a flip control unit, which is connected to the flip motor through a signal; Optionally, it also includes an automatic sample carrier transfer device, which includes a first robot arm assembly, The first mechanical arm assembly includes a mechanical arm, a rotating arm arranged on the mechanical arm and rotating between the Y direction and the Z direction, and a flip control unit. The flip control unit is connected to a flip motor in the flip mechanism through a signal.

[0019] Optionally, the automatic sample carrier transfer device further comprises a third mechanical arm assembly and a transfer control unit, the third mechanical arm assembly is provided with a second absorber, and the second absorber can absorb and transfer more than one sample carrier at the same time; The transfer control unit is connected to the transfer motor in the third mechanical arm assembly through a signal to control the rotation of the third mechanical arm.

[0020] Optionally, the first robot arm assembly includes a first X-direction moving group, a first Y-direction moving group and a first Z-direction moving group; the first Y-direction moving group is provided with a rotating arm rotating between the Y direction and the Z direction.

[0021] Optionally, it also includes a transfer module for accommodating more than one sample carrier.

[0022] Optionally, it further comprises a liquid adding device, wherein the liquid adding device comprises a liquid adding body and a reagent component and a cleaning component arranged on the liquid adding body; The reagent assembly includes a liquid transfer gun and a reagent driving mechanism arranged on a liquid adding body; The reagent driving mechanism drives the pipette gun to move up and down relative to the liquid adding body Z, so that the tip of the pipette gun extends downward and is recovered; The cleaning component includes a liquid suction pipe, an air blowing pipe, a liquid outlet pipe, and a cleaning drive mechanism arranged on the liquid adding body; the cleaning drive mechanism drives the cleaning component to move upward and downward relative to the liquid adding body Z; In this solution, the pipette moves up and down along the Z direction under the drive of the reagent drive mechanism to achieve accurate addition of the reagent. The pipette, air blow pipe and liquid outlet pipe are driven by the cleaning drive mechanism to achieve the cleaning operation of the sample. The whole process can be controlled by the transfer control unit.

[0023] Optionally, it also includes a liquid adding control unit, which is connected to the reagent motor of the reagent driving mechanism and the cleaning motor of the cleaning driving mechanism through signals to drive the movement of the cleaning component and the reagent component respectively.

[0024] Optionally, it further comprises a second robot arm assembly, wherein the second robot arm assembly comprises a second X-axis moving group and a second Y-axis moving group arranged on the second X-axis moving group; the liquid adding device is arranged on the second Y-axis moving group.

[0025] Optionally, it also includes a fixedly arranged pipette tip rack, on which a plurality of slots for placing the tips and a plurality of tips mounted on the slots are arranged, and the plurality of tips are arranged to adsorb corresponding different reagents.

[0026] In this scheme, a quick operation for replacing multiple reagents is provided to prevent cross contamination and improve experimental efficiency.

[0027] Optionally, the incubation device comprises an incubation rack, the incubation rack comprises a plurality of parallel incubation trays, each incubation tray is connected to an adjacent incubation tray via at least one supporting structure; each incubation tray can be pushed out independently. Each incubation tray can be pushed out independently, so as to realize flexible processing of samples and ensure effective incubation under different experimental requirements.

[0028] Among them, the independent pushing out of the incubation tray and the cooperation of the flipping mechanism can realize the pre-addition of reagents in the incubation tray. The flipping mechanism flips and moves the sample carrier so that the side carrying the sample is inverted and attached to the incubation tray, promoting the contact between the reagent and the sample to achieve sample addition or liquid addition; maintain and continue to attach for incubation.

[0029] Optionally, the incubation rack is further provided with a blowing tray, which can be independently pushed out relative to the incubation tray; and the blowing tray is provided with a blowing port. The blowing tray is independently pushed out to provide a blowing function, thereby ensuring that the reagent is evenly attached and reducing the interference of residual liquid on the detection.

[0030] Optionally, the incubation rack is further provided with a cleaning tray, and the inner side wall of the cleaning tray is provided with a water outlet. The water outlet inside the cleaning tray can ensure thorough cleaning of the sample carrier, reduce interference from impurities, and provide a clean environment for subsequent reactions.

[0031] Optionally, it also includes a third robotic arm assembly, which can adsorb and transfer more than one sample carrier at the same time; it can process multiple sample carriers at the same time, shorten the operation time, and significantly improve the system efficiency.

[0032] Optionally, it also includes a transfer module for accommodating more than one sample carrier.

[0033] In a second aspect, the present invention provides a high pressure immunohistochemistry method, comprising the following steps: S1 Dewaxing and heat repair of sample carriers containing samples; S2: incubating more than once, the incubation method comprising: turning over the sample carrier (10) so that the side carrying the sample covers the container with the reagent so that the reagent and the sample are attached to each other, and then incubating after adding liquid; S3 color development reaction: adding color development reagent to the incubated sample carrier to carry out color development reaction.

[0034] In this scheme, the flipping operation makes the sample away from the surface side or other non-specific substances participating in the incubation and other reaction steps, thereby reducing noise and improving specific reaction.

[0035] Optionally, it further includes the following steps: In step S2, the sample carrier is cleaned after incubation, and the cleaning method includes: turning over the sample carrier to expose a side carrying the sample, and cleaning with water.

[0036] Optionally, dewaxing and thermal repair are performed in a thermal repair reaction device; The sample carrier is taken out from the thermal repair reaction device by means of the flipping mechanism, and the sample carrier is flipped so that the side carrying the sample is inverted; The sample carrier is moved to the incubation device by the flipping mechanism to perform steps including liquid addition and incubation.

[0037] Optionally, the one or more incubations include primary antibody incubation; the sample carrier is added with a primary antibody reagent for primary antibody incubation; Optionally, the one or more incubations further include secondary antibody incubation; the sample carrier is added with a secondary antibody reagent for secondary antibody incubation; Optionally, the primary antibody reagent may be selected as an amplifier or enhancer; Optionally, the color developing reagent is a DAB reagent.

[0038] Optionally, it also includes the following steps: In step S2, the sample carrier is cleaned after incubation, and the cleaning method includes: turning over the sample carrier to expose the side carrying the sample, and cleaning with water.

[0039] Optionally, the dewaxing and thermal repair are carried out in a thermal repair reaction device; The sample carrier is taken out from the thermal repair reaction device by means of the flipping mechanism, and the sample carrier is flipped so that the side carrying the sample is inverted; The sample carrier is moved to the incubation device by the flipping mechanism to perform steps including liquid addition and incubation.

[0040] In this solution, the flipping mechanism can accurately move the sample carrier between the thermal repair reaction device and the incubation device, realize the continuous operation of incubation and color development reaction, and improve the degree of automation of the experiment.

[0041] (III) Beneficial effects The beneficial effects of the present invention are: The high-pressure immunohistochemistry system of the present invention is suitable for high-pressure immunohistochemistry methods. By improving the moisture retention and coverage of reagents, the efficiency and quality of immunohistochemistry experiments are significantly improved, and the problems of reagent evaporation and sample drying in traditional technologies are solved. It is an innovative design with significant advantages.

[0042] Among them, the special flipping mechanism is set up to prevent the loss and evaporation of reagents: the sample carrier is inverted and covered in the container containing the reagent, which avoids the reagent from being directly exposed to the air, reduces evaporation, and ensures that the reagent is in full contact with the sample surface during incubation, thereby improving the stability of the experiment; reducing non-specific reactions: the inverted design allows the sample to be in full contact with the reagent in the container while isolating the air, effectively reducing the chance of non-antigen substances reacting with the reagent, thereby reducing noise and improving sensitivity; more efficient incubation process: the reagent can cover the sample more evenly and fully, ensuring the effect and consistency of incubation, and enhancing the sensitivity and accuracy of the immunohistochemical reaction.

[0043] Among them, the present invention further performs dewaxing and thermal repair through a thermal repair reaction device, which has a closed cavity and can achieve heating and pressurization at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 It is a schematic diagram of the structure of the high-pressure immunohistochemistry system of the present invention; Figure 2 It is a structural schematic diagram of the first mechanical arm assembly and the flipping mechanism of the present invention; Figure 3It is a structural schematic diagram of the thermal repair reaction device of the present invention; Figure 4 A more specific structural schematic diagram of the thermal repair reaction device of the present invention; Figure 5 It is a structural schematic diagram of the first sample carrier placement area of ​​the present invention; Figure 6 It is a structural schematic diagram of the liquid adding device of the present invention; Figure 7 It is a schematic diagram of the structure of the incubation device of the present invention; Figure 8 It is a schematic structural diagram of the first mechanical arm assembly, the second mechanical arm assembly and the third mechanical arm assembly of the present invention; Fig. 9 It is a structural schematic diagram of the reagent storage tank of the present invention; Fig.10 It is a schematic diagram of the structure of the push-pull member of the present invention; Fig.11 It is an enlarged schematic diagram of the structure of the blowing plate of the present invention; Fig.12 It is an enlarged schematic diagram of the structure of the cleaning disk of the present invention.

[0045] The reference numerals in the above drawings are described as follows: 1. Thermal repair reaction device; 11. Reactor body; 111. Reactor wall; 112. First water outlet; 113. Water inlet; 114. Exhaust port; 115. Temperature probe; 116. Heating element; 117. Accommodating cavity; 118. Top cover; 119. Bottom cover; 12. First sample carrier placement area; 13. Tank body; 14. Lifting mechanism; 15. Stepping motor; 16. Screw rod; 17. Bracket; 18. Support rod; 19. Connecting rod; 2. Incubation device; 21. First incubation rack; 22. Second incubation rack; 23. Incubation tray; 24. Air blowing tray; 25. Air blowing port; 26. Push-pull member; 261. Push-pull rod; 262. Snap-fit ​​block; 263. Snap-fit ​​hole; 27. Cleaning tray; 28. Second water outlet; 29. ​​Pipette tip rack; 3. First mechanical arm assembly; 31. Flip mechanism; 311. Rotating arm; 312. Flip motor; 313. First suction member; 32. First X-direction moving group; 33. First Y-direction moving group; 34. First Z-direction moving group; 4. Workbench; 41. Crossbar; 5. Reagent storage tank; 6. Liquid adding device; 61. Liquid adding body; 62. Reagent assembly; 621. Liquid transfer gun; 63. Cleaning assembly; 631. Liquid pipette; 632. Air blowing tube; 633. Liquid outlet tube; 7. Second mechanical arm assembly; 71. Second mechanical arm; 8. The third mechanical arm assembly; 81. The third Y-axis moving group; 82. The third Z-axis moving group; 83 second suction member; 9. Transfer module; 10. Sample carrier. DETAILED DESCRIPTION

[0046] In order to better explain the present invention, so as to facilitate understanding, exemplary embodiments of the present invention will be described in more detail below. Although exemplary embodiments of the present invention are shown below, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided in order to be able to more clearly and thoroughly understand the present invention, and to be able to fully convey the scope of the present invention to those skilled in the art.

[0047] Reference to "embodiment" herein means that the specific features, structures or characteristics described in conjunction with the embodiment may be included in at least one embodiment of the present application. The term "embodiment" appearing in various places in the specification does not necessarily refer to the same embodiment, nor does it particularly limit its independence or association with other embodiments. In principle, in the present application, as long as there is no technical contradiction or conflict, the various technical features mentioned in the embodiments can be combined in any way to form a corresponding implementable technical solution.

[0048] Unless otherwise defined, the technical terms used in this document have the same meanings as those generally understood by those skilled in the art to which this application belongs; the use of relevant terms in this document is only for describing specific embodiments and is not intended to limit this application.

[0049] In the description of this application, the term "and / or" is an expression used to describe the logical relationship between objects, indicating that three relationships may exist, for example, A and / or B, which means: A exists, B exists, and A and B exist at the same time. In addition, the character " / " in this article generally indicates that the objects before and after are in an "or" logical relationship.

[0050] In the present application, terms such as “first” and “second” are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship of quantity, priority or sequence between these entities or operations.

[0051] Without further limitations, in this application, the words "include", "comprises", "has" or other similar expressions used in the sentences are intended to cover non-exclusive inclusion. These expressions do not exclude the presence of additional elements in the process, method or product including the elements, so that the process, method or product including a series of elements may include not only those limited elements, but also other elements not explicitly listed, or also include elements inherent to such process, method or product.

[0052] Similar to the understanding in the Examination Guidelines, in this application, expressions such as "greater than", "less than", "exceed" and the like are understood to exclude the number itself; expressions such as "above", "below", "within" and the like are understood to include the number itself. In addition, in the description of the embodiments of this application, "multiple" means more than two (including two), and similar expressions related to "multiple" are also understood in this way, such as "multiple groups", "multiple times", etc., unless otherwise clearly and specifically limited.

[0053] In the description of the embodiments of the present application, space-related expressions used, such as "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "vertical", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicate the orientation or position relationship based on the orientation or position relationship shown in the specific embodiments or drawings, and are only for the convenience of describing the specific embodiments of the present application or facilitating the reader's understanding, and do not indicate or imply that the referred device or component must have a specific position, a specific orientation, or be constructed or operated in a specific orientation, and therefore cannot be understood as a limitation on the embodiments of the present application.

[0054] Unless otherwise expressly specified or limited, in the description of the embodiments of the present application, the terms such as "install", "connect", "connect", "fix", "set", etc. used should be understood in a broad sense. For example, the "connection" can be a fixed connection, a detachable connection, or an integrated setting; it can be a mechanical connection, an electrical connection, or a communication connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium; it can be the internal connection of two elements or the interaction relationship between two elements. For technicians in the technical field to which the present application belongs, the specific meanings of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0055] It should be noted that the sample carrier described in the present invention includes but is not limited to a glass slide, and may include any container or carrier having a side on which a sample can be placed.

[0056] The X direction, Y direction and Z direction referred to in the present invention do not only refer to the directions of the arrows on the X axis, Y axis and Z axis in the drawings, but may be the opposite directions indicated by the arrows on the X axis, Y axis and Z axis.

[0057] Example 1 like Figure 1 As shown: This embodiment provides a high-pressure immunohistochemistry system, which includes a thermal repair reaction device 1, an incubation device 2, and a flipping mechanism 31; the thermal repair reaction device 1 and the incubation device can both accommodate a sample carrier 10; the flipping mechanism 31 is used to flip and / or move the sample carrier 10.

[0058] The flip mechanism 31 in this embodiment is used to flip and / or move the sample carrier 10, and the sample carrier 10 is accurately positioned at different operation stages through the cooperation of the thermal repair reaction device 1, the incubation device and the flip mechanism 31. The flip mechanism 31 can ensure that the sample is inverted and covered during the liquid addition and incubation process, thereby reducing the loss and evaporation of reagents.

[0059] The flipping mechanism 31 in this embodiment can flip the sample carrier 10, so that the sample carrier 10, such as a glass slide, with the side of the sample such as tissue facing downward, is moved to the incubation device for incubation. The incubation device 2 has a first sample carrier placement area, and the reagent to be reacted is dripped in advance in the first sample carrier placement area. After the flipping mechanism 31 flips the sample carrier 10 upside down, the side of the sample carrier 10 with the sample such as tissue facing downward covers any area with the reaction reagent, and the reagent is added, while continuing to incubate the reaction in a covered manner.

[0060] In this embodiment, the flipping mechanism 31 may be any other structure that can flip the sample carrier 10 upside down so that the side of the sample carrier 10 with the tissue sample faces downward.

[0061] In some other specific embodiments, Figure 2 As shown, the flipping mechanism 31 includes a rotating arm 311, a flipping motor 312 and a first suction piece 313. The end of the rotating arm 311 is provided with a first suction piece 313 for adsorbing the sample carrier 10. The first suction piece 313 is used to adsorb and put down the sample carrier 10. The output shaft of the flipping motor 312 is connected to the rotating shaft of the rotating arm 311 to drive the rotation of the rotating arm 311.

[0062] In some other specific embodiments, the flip mechanism 31 is driven to move by a mechanical arm, and the mechanical arm and the flip structure 31 constitute a first mechanical arm assembly 3 in the automatic slide transfer module, wherein the first mechanical arm assembly 3 also includes a first X-direction moving group 32, a first Y-direction moving group 33, and a first Z-direction moving group 34; the first Y-direction moving group 33 is provided with a rotating arm 311 that can rotate between the Y-direction and the Z-direction, and the output shaft of the flip motor 312 is connected to the sample carrier 10 of the rotating arm 311. In some other specific embodiments, the high-pressure immunohistochemistry system also includes a workbench 4, and the thermal repair reaction device 1, the incubation device 2, and the mechanical arm are arranged on the workbench 4. The flip mechanism 31 is connected to the workbench 4 through the mechanical arm.

[0063] In some other specific embodiments, the first mechanical arm assembly 3 further includes a flip control unit. The flip control unit is connected to the flip motor 312 in the flip mechanism 31 through a signal.

[0064] In some other specific embodiments, the first suction member 313 is one or more suction cups arranged at the end of the rotating disk. A vacuum interface is arranged in the suction cup, which is connected to a vacuum pump pipeline. The vacuum pump controls the switch through the first electromagnetic valve to realize the suction cup sucking and putting down the glass slide; wherein the first electromagnetic valve is controlled by the suction control unit to control its sucking and putting down.

[0065] Example 2 like Figure 3 , 4 As shown in Figure 5, this embodiment provides a high-pressure immunohistochemistry system. Compared with Example 1, the thermal repair reaction device 1 includes: It includes a reactor body 11, a heating element 116, a temperature probe 115, a sample carrier placement area 12, a lifting mechanism 14 and a thermal repair control unit. The reactor body 11 needs to be able to meet the dewaxing and repairing conditions of immunohistochemistry in the biomedical field, such as heating uniformity, durability and safety, easy cleaning, and compatibility with commonly used immunohistochemical reagents and solutions. It can be made of materials such as stainless steel, aluminum alloy, ceramic, etc., and the shape is not particularly limited, including but not limited to cylindrical, square, rectangular, but preferably a stainless steel cylindrical pot. The reactor body 11 has a reactor wall 111, a top cover 118 and a bottom cover 119. The reactor wall 111 and the bottom cover 119 enclose a receiving cavity 117, and the receiving cavity 117 is used to accommodate the test sample. In the dewaxing and repairing process, the test sample is the glass slide group to be dewaxed and repaired. The heating element 116 is, for example, an electric heating wire, an electric heating tube, an electric heating plate, an electromagnetic coil, an electric heating film, a graphite heating element, etc., which is separately arranged on the reactor wall 111 or the accommodating cavity 117, or is simultaneously arranged on the reactor wall 111 and the accommodating cavity 117. It is preferably separately arranged on the reactor wall 111 to avoid occupying space in the accommodating cavity 117 and unnecessary test risks. The temperature probe 115 is arranged on the top cover 118. The first sample carrier placement area 12 in this embodiment is a box or basket-shaped slide rack made of a material that can withstand high temperatures above 60°C and has a slide limiting structure that can place the slide horizontally or vertically. The lifting mechanism 14 includes a stepper motor 15, a screw rod 16, a bracket 17, a support rod 18, and some screws, nuts and other accessories for fixing. The stepper motor 15 is fixedly arranged on the bracket 17, and is connected to the top cover 118 located directly below the stepper motor 15 through the screw rod 16. The top cover 118 is connected to the first sample carrier placement area 12 through the connecting rod 19. The maximum distance between the top cover 118 and the first sample carrier placement area 12 is less than or equal to the depth of the accommodating cavity 117. In this way, when the top cover 118 is lowered along the screw rod 16, the glass slide placement area is gradually lowered into the accommodating cavity 117, and it can be ensured that the top cover 118 can be completely closed with the upper part of the reactor body 11. The support rod 18 penetrates the edge of the top cover 118 to support the bracket 17, and is used for the top cover 118 to move up and down along the support rod 18. The thermal repair control unit includes a temperature control module and a drive module. The temperature control module is electrically connected to the temperature probe 115 and is used to control the heating element 116 based on the temperature reading of the temperature probe 115; the drive module is electrically connected to the lifting mechanism 14 and is used to drive the lifting mechanism 14 to move upward or downward.

[0066] These embodiments realize the automation of the thermal repair process, automatically expose or close the slide placement area, effectively reduce the complexity and potential errors of manual operation, shorten the total time required for thermal repair, and are of great significance for speeding up the experimental process and improving laboratory work efficiency.

[0067] Example 3 This embodiment provides a high-pressure immunohistochemistry system, which is different from the embodiment 1. Figure 6 As shown, it also includes a liquid adding device 6, The liquid adding device 6 includes a liquid adding body 61 and a reagent assembly and a cleaning assembly arranged on the liquid adding body 61; the reagent assembly includes a liquid transfer gun 621 and a reagent driving mechanism arranged on the liquid adding body 61; The reagent driving mechanism drives the pipette gun 621 to move up and down relative to the liquid adding body 61, so that the tip of the pipette gun 621 extends downward and is recovered; In some other specific embodiments, the reagent driving mechanism includes a reagent motor, a rotating shaft, a gear and a driving belt. The output shaft of the reagent motor is connected to the rotating shaft. The upper and lower ends of the liquid adding body 61 are respectively provided with rotating shafts. Gears are fixedly provided on the rotating shafts. A driving belt is provided on the gears. Part of the driving belt is fixedly connected to the pipetting gun 621. In some other specific embodiments, the pipette gun 621 includes a gun body, a pipette tip, a pipette button, a pipette tip ejection button, and an electric telescopic mechanism, wherein the pipette tip is disposed below the gun body, a pipette tip connector, a piston mechanism, and an ejection mechanism control element; The telescopic end of the electric telescopic mechanism faces downward and directly faces the top of the liquid aspiration button. The electric telescopic mechanism is connected to a power source through a controller. The controller controls the telescopic mechanism to extend and retract, and transmits a control signal to the controller.

[0068] In some other specific embodiments, Figure 7 As shown, it also includes a fixedly arranged pipette tip rack 29; the pipette tip rack 29 is provided with a plurality of slots for placing the tips and a plurality of tips arranged on the slots, and the plurality of tips are arranged to adsorb different reagents accordingly. The pipette tip rack 29 can be fixedly arranged on the workbench 4.

[0069] In some other specific embodiments, like Figure 6 As shown, the cleaning component includes a liquid suction pipe 631, an air blowing pipe 632, a liquid outlet pipe 633, and a cleaning drive mechanism arranged on the liquid adding body 61; the cleaning drive mechanism drives the cleaning component to move up and down in the Z direction relative to the liquid adding body 61; In some other specific embodiments, the cleaning assembly includes a liquid suction pipe 631, an air blowing pipe 632, a liquid outlet pipe 633, a water tank, a waste liquid tank and a reagent driving mechanism arranged on the liquid adding body 61. The water tank and waste liquid tank are detachably arranged on the liquid adding body 61. The other end of the liquid outlet pipe 633 is connected to the liquid outlet of the water tank, and a first valve is provided on the liquid outlet pipe 633, and the pipe opening of the liquid outlet pipe 633 is arranged downward; the other end of the liquid suction pipe 631 is connected to the liquid inlet of the waste liquid tank, and a second valve is provided on the liquid suction pipe 631, and the pipe opening of the liquid suction pipe 631 is arranged downward; the pipe opening of the air blowing pipe 632 is arranged downward; the pipe openings of the liquid outlet pipe 633, the liquid suction pipe 631 and the air blowing pipe 632 are arranged to be not in the same horizontal plane; preferably, the pipe opening of the liquid suction pipe 631 is arranged at the lowest end, and the pipe opening of the liquid outlet pipe 633 is arranged at the highest end; In some other specific embodiments, it also includes a cleaning control unit, which controls the opening and closing of the first valve and the second valve through signals.

[0070] In some other specific embodiments, Figure 1 , 7 As shown in FIG. 8 , the liquid adding device 6 is further provided. The second mechanical arm assembly 7 includes a second X-direction moving group and a second Y-direction moving group arranged on the second X-direction moving group. The second Y-direction moving group is provided with the liquid adding device 6. The second mechanical arm assembly 7 drives the liquid adding device 6 to move in a three-dimensional direction, so that the liquid adding device 6 moves to a desired position to add reagents.

[0071] In some other specific embodiments, Figure 1 and 7 The workbench shown is a three-dimensional workbench 4, and the second X-direction moving group includes a crossbar 41 arranged on the three-dimensional workbench 4, and a second mechanical arm 71 driven by a second X-direction motor to move on the crossbar 41; The second Y-direction moving group includes a second Y-direction slider disposed on the second mechanical arm 71 and sliding along the second mechanical arm 71 and a second Y-direction motor driving the second Y-direction slider; the liquid adding device 6 is connected to the second Y-direction slider.

[0072] In some other specific embodiments, it also includes a liquid adding control unit, which is connected to the reagent motor of the reagent driving mechanism and the cleaning motor of the cleaning driving mechanism through signals to drive the movement of the cleaning component and the reagent component respectively; it is also connected to the second X-direction motor and the second Y-direction motor through signals to control the movement of the liquid adding component in the X and Y directions.

[0073] In some other specific embodiments, Fig. 9 As shown, the workbench 4 is also provided with a plurality of reagent storage slots 5, corresponding to which fixed reagent bottles are placed. The liquid adding control unit is connected to the electric telescopic mechanism through a signal, and the liquid adding control unit controls the second X-direction motor and the second Y-direction motor 73 and the electric telescopic mechanism to control the liquid adding device 6 to absorb a specific reagent at a specific position of the reagent storage slot 5 for liquid adding.

[0074] The high-pressure immunohistochemistry system provided in this embodiment can automatically complete the sample processing, incubation and cleaning processes by integrating the thermal repair reaction device 1, the incubation device 2 and the robotic arm device on a workbench 4, thereby significantly improving the experimental efficiency and the sample processing speed.

[0075] The system uses a robotic arm device to automatically transfer sample carriers and distribute reagents, reducing human operations during the experiment, thereby reducing possible operational errors and improving the accuracy and repeatability of experimental results.

[0076] Automation: users only need to set relevant parameters to automatically complete the entire experimental process, reducing the complexity of the operation.

[0077] This embodiment can not only effectively improve the automation level and efficiency of the experiment, but also ensure the accuracy and reliability of the experimental results, and is suitable for high-throughput biomedical research and clinical diagnosis.

[0078] In some other specific embodiments, the workbench 4, the thermal repair reaction device 1, the incubation device 2, the reagent assembly and the cleaning assembly can be structures recorded in the prior art with the same functions as those in the present embodiment.

[0079] Example 4 This embodiment provides a high-pressure immunohistochemistry system, which, relative to embodiments 1-3, further comprises: Figure 7 As shown, an incubation device comprises a first incubation rack 21 and a second incubation rack 22, wherein the first incubation rack 21 and the second incubation rack 22 each comprise a plurality of parallel incubation trays 23, each incubation tray 23 is connected to an adjacent level via at least one supporting structure, and each incubation tray 23 is provided with a plurality of slots for placing sample carriers 10, forming a first sample carrier placement area; each incubation tray 23 can be independently pushed out.

[0080] The first incubation rack 21 or the second incubation rack 22 is also provided with an air blowing tray 24 parallel to the incubation tray 23. Fig.10 and 11 As shown, the blowing plate 24 can be pushed out independently relative to the incubation plate 23; the blowing plate 24 is provided with a blowing port 25, and the blowing port 25 is configured as a directional airflow, preferably vertically upward, and the blowing plate 24 is connected to the incubation rack through two guide rails, and the guide rails are controlled by a motor to push the blowing plate 24 along the guide rails, and the motor is controlled by a controller.

[0081] The air port 25 is connected to a compressed air source or an air pump through an air pipe 632; The push-pull member 26 is connected to the incubation tray 23 and is used to push the incubation tray 23 out; The controller is connected to the compressed air source or air pump through a signal to control the opening, closing and operation intensity of the compressed air source or air pump; The controller is connected to the transfer motor through a signal, and controls the motor to drive the push-pull member 26 forward.

[0082] like Fig.12 As shown, a cleaning tray 27 is further provided at the lower end of the first incubation rack 21 or the second incubation rack 22 , and a second water outlet 28 is provided on the inner side wall of the cleaning tray 27 .

[0083] In some other specific embodiments, the push-pull member 26 is disposed between the first incubation rack 21 and the second incubation rack 22, and each incubation tray 23 is provided with a snap-fit ​​hole 263; like Fig. 9 As shown, the push-pull member 26 includes a push-pull member 26 body, a first slider disposed on the push-pull member 26 body, and a second lifting structure, the first slider is connected to a push-pull rod 261 on its left and right sides respectively, the push-pull rod 261 is provided with a snap-fit ​​block 262 that matches the snap-fit ​​hole 263, the snap-fit ​​block 262 is an L-shaped structure extending out of the push-pull rod 261, and one end of the L-shape is arranged downward; Optionally, the first slider includes a left slider and a right slider, and the left slider and the right slider are respectively connected to a push-pull rod 261 .

[0084] The push-pull member body can optionally drive the left and right sliders to move up and down in the Z direction through the second lifting structure, so that the push-pull rod 261 can be selectively engaged with any one of the incubation trays 23, and the push-pull member body can move in the Y direction through the Y-direction moving module, so that the push-pull rod 261 can drive the incubation tray 23 to be pushed out.

[0085] The controller controls the lifting and lowering of the second lifting mechanism 14 by connecting the transfer motor via a signal, and controls the Y-direction movement of the push-pull member 26 by connecting the Y-direction movement module via a signal.

[0086] The second lifting mechanism includes: a second stepper motor, a second driving screw and a guide rail, the shaft of the second stepper motor is directly connected to the second driving screw; the second driving screw converts the rotational motion of the second stepper motor into linear motion. The second screw passes through a fixed nut, which is fixed on the second slider but allows the second screw to rotate freely. When the second stepper motor rotates, the second screw does not rotate, but drives the nut and the attached second slider to move along the axial direction of the second screw.

[0087] In order to ensure the stability and precision movement of the second slider in the Z direction, the second lifting mechanism is equipped with two parallel guide rails. The second slider is equipped with ball bearings or second sliders that match the guide rails. These bearings or second sliders surround the guide rails to provide smooth, low-friction movement.

[0088] Among them, the Y-axis moving module includes: servo motor, gear and guide rail. The Y-axis moving module is powered by a servo motor equipped with a high-precision encoder to monitor and control position and speed to ensure accurate movement.

[0089] The power of the servo motor is transmitted to a rack meshed with it through a gear connected to its shaft. The rack is fixed on the workbench 4 and is extended in the direction of the preset movement of the Y-axis in the Y-direction moving module. The rotation of the gear causes the main body of the push-pull member 26 connected to the rack to move along the Y-axis.

[0090] The guide rail is used to keep the main body of the push-pull member 26 moving linearly and stably, and the main body of the push-pull member 26 interacts with the guide rail through a ball bearing or a sliding bearing.

[0091] The main body of the push-pull member 26 is connected to the first slider connecting arm through one or more transverse connecting arms, allowing the linear movement of the second lifting structure in the Z direction to cooperate with the linear movement of the moving module in the Y direction to accurately control the position of the incubation tray 23.

[0092] Example 5 like Figure 1 and 8 As shown, this embodiment provides a high-pressure immunohistochemistry system. Compared with Embodiments 1 to 4, the automatic transfer module further includes a third mechanical arm assembly 8 and a transfer module 9. The third robot arm assembly 8 includes a third Y-axis moving group 81 and a third Z-axis moving group 82 arranged on the third Y-axis moving group; and can absorb and transfer more than one sample carrier 10 at the same time.

[0093] In some specific embodiments, the first suction member 313 and the second suction member 83 are suction cup structures. The second suction member 83 is arranged on the third Z-direction moving group 82, and includes one or more suction cups, and the suction cups are arranged downward. One or more suction cups can cooperate with one or more sample carriers 10 placed on the transfer module 9 to absorb at the same time. If the sample carrier 10 is a glass slide, the glass slide is placed in a vertical manner on the thermal repair reaction device 1. The controller is connected to the cleaning drive mechanism through a signal to drive the suction and release of the suction cup.

[0094] The first suction member 313 and the second suction member 83 are suction cup structures, which are designed to generate sufficient negative pressure to stably absorb the vertically placed glass slide sample carrier 10. The suction cups are connected to a vacuum pump through a hose, and the switch of the vacuum pump is controlled by a controller signal to ensure that the suction cups can absorb or release the glass slide at the appropriate time.

[0095] The controller is connected to the electric telescopic mechanism, the flip control unit, the transfer control unit, the thermal repair control unit, and the suction control unit through signals. When in operation, the controller controls the first suction member of the first mechanical arm assembly to absorb the vertically placed sample carrier 10 from the thermal repair reaction device 1, in particular, to absorb the sample carrier 10 on the side that does not carry the sample, and flips the sample carrier 10 to be placed horizontally through the flip mechanism of the first mechanical arm assembly. Optionally, the side carrying the sample is covered on the transfer module 9 upside down, and the above steps are repeated until a set number of sample carriers 10 are on the transfer module 9. Start the second suction member of the third robotic arm assembly, wherein the suction cup of the second suction member is configured to fit the positions of the multiple sample carriers 10 on the transfer module 9, and can simultaneously absorb the multiple sample carriers 10; the third robotic arm assembly transfers the multiple sample carriers to the incubation tray of the incubation device, and the third robotic arm assembly is not provided with a Y-direction moving module, because the incubation tray in the incubation device will be pushed out in the Y direction. Start the third robotic arm assembly, move the pipette in the liquid adding device to the pipette storage rack, extend the pipette downward, and after the pipette tip corresponding to the reagent is set on the pipette storage rack, retract the pipette; move the third robotic arm assembly to the reagent storage tank, extend the pipette tip downward, absorb the preset amount in the controller, and then move to the incubation device to add the reagent to the incubation tray in advance; the third robotic arm assembly covers the sample carrier upside down in the reagent, and incubates at the same time. After the incubation is completed, the third robot arm assembly transfers the multiple sample carriers to another incubation tray, preferably to an incubation tray of another incubation rack, and optionally to a cleaning tray so that the sample carriers are attached to the bottom of the cleaning tray. The water outlet of the cleaning tray is set on the inner wall, and the direction of the water outlet is horizontal in the Y direction, which is adapted to the position where each glass slide is placed. After cleaning, the third robot arm assembly transfers the sample carrier to the top of the blowing port of the blowing tray, and the motor of the blowing tray is activated. When the air pump is started to blow, the blowing tray moves back and forth along the guide rail in the Y-axis direction to achieve blowing. After the blowing is completed, it is transferred to a clean incubation tray. At the same time, the third mechanical arm assembly is moved to clean the incubated incubation tray through the cleaning assembly; the cleaning assembly is started to make the liquid outlet pipe flow water onto the incubation tray; preferably, the incubation tray forms multiple sample carrier incubation areas with the sample carrier through multiple grooves, and the movement of the cleaning assembly is controlled so that the liquid outlet pipe can move and spray along the extension direction of the groove while discharging liquid; after the cleaning is completed, the cleaning assembly is moved downward so that the liquid pipette touches the incubation tray to absorb liquid; optionally, a liquid extraction pipe is provided in the cleaning tray, and the liquid extraction pump is controlled by the controller to extract water. After the incubation tray absorbs liquid, the cleaning assembly is retracted upward, and the air blowing pipe is controlled to exhaust air to blow away the water droplets on the incubation tray; after the cleaning and air blowing are completed, it is used as a clean incubation tray to prepare for the next step of adding reagents and incubation.

[0096] This embodiment can be applied to the full automation of any high-pressure immunohistochemical method, and can be applied to all high-pressure immunohistochemical methods by setting corresponding parameters of the controller.

[0097] Transfer drive mechanism: responsible for controlling the suction and release of the suction cup of the third robot arm assembly 8. It includes a small motor that rotates through a gear and linkage mechanism to push the suction cup to move toward or away from the glass slide surface. The movement of the motor is fed back to the controller through an encoder to ensure accurate control of the position of the suction cup.

[0098] The above controller is provided with relevant control units (including but not limited to electric telescopic mechanism, flip control unit, transfer control unit, thermal repair control unit, suction control unit, thermal repair control unit), which transmits instructions to relevant drive mechanisms including but not limited to transfer drive mechanism, reagent drive mechanism, cleaning drive mechanism, transfer motor through electrical signals to control the precise movements of the pipette gun 621, cleaning assembly and suction cup, etc. After receiving the signal, the relevant drive mechanism activates the corresponding motor or solenoid valve through the internal circuit to perform the required action.

[0099] Integration of control systems: The control system integrates sensor data such as position sensors, pressure sensors, etc., monitors the status of the robot arm in real time, and adjusts operating parameters to ensure accuracy and repeatability of operations.

[0100] Example 6 This embodiment also provides a method for high-pressure immunohistochemistry liquid addition and incubation reaction, which includes the following steps: placing the sample carrier 10 carrying the sample upside down in a container pre-added with reagents to perform liquid addition and incubation reaction.

[0101] In the prior art, during the process of adding reagents and incubating, the sample carrier 10, such as a glass slide, is placed with the side with tissue or other samples facing upward, and at most, is only covered with a film.

[0102] There is a risk that the reagent may evaporate or directly leak from the sample carrier 10 such as a glass slide. After the reagent leaks, the tissue is directly exposed to the air and cannot be moisturized, and may dry out, which may easily lead to the failure of the experiment.

[0103] This embodiment adopts an inverted cover type, which can avoid reagent loss and reduce evaporation, while reducing the reaction between non-antigen substances and reagents during incubation, reducing nonspecific reactions, reducing noise, and improving sensitivity.

[0104] Example 7 This embodiment provides a high-pressure immunohistochemistry method, which comprises the following steps: S1 Dewaxing and heat repair of sample carriers containing samples; S2 primary antibody incubation: adding primary antibody reagent to the sample carrier for primary antibody incubation; S3 secondary antibody incubation: add secondary antibody reagent to the sample carrier for secondary antibody incubation; S4 color reaction; Methods for adding a primary antibody reagent or a secondary antibody reagent include: The sample carrier is turned over so that the loaded side is covered in the container with the primary antibody reagent and / or the secondary antibody reagent.

[0105] The flipping operation allows the sample to move away from the surface side or other non-specific substances involved in incubation and other reaction steps, reducing noise and improving specific reactions.

[0106] More specifically: S1 dewaxing and heat repairing of a sample carrier (10) carrying a sample; S5 color development reaction; adding DAB reagent to the sample carrier (10) for DAB incubation; Wherein, the secondary antibody reagent may be, but is not limited to, an amplifier or enhancer; Wherein, in the color development reaction, the loaded reagent may be but is not limited to DAB reagent; Optionally, it also includes the following steps: After any one of steps S1 to S4, the method of adding other reagents to the sample carrier for reaction includes: turning over the sample carrier so that the side carrying the sample is covered with a container with other reagents for reaction; or / and The following steps are also included: The sample carrier is cleaned, and the cleaning method includes: turning over the sample carrier to expose a side carrying the sample, and cleaning with water.

[0107] Optionally, the dewaxing and thermal repair are carried out in a thermal repair reaction device; The sample carrier is taken out from the thermal repair reaction device by means of the flipping mechanism, and the sample carrier is flipped so that the side carrying the sample is inverted; The sample carrier is moved to the incubation device by the flipping mechanism to perform liquid addition and incubation steps including primary antibody incubation and secondary antibody incubation.

[0108] The flipping mechanism can accurately move the sample carrier between the thermal repair reaction device and the incubation device, realize the continuous operation of incubation and color development reaction, and improve the degree of automation of the experiment.

[0109] This embodiment provides a high-pressure immunohistochemistry method, which comprises the following steps: S1: dewaxing and thermally repairing the sample carrier 10 carrying the sample in the thermal repair reaction device 1; S2 uses the first suction cup in the first mechanical arm assembly 3 to suck the sample carrier 10 from the thermal repair reaction device 1 and place it on the transfer module 9; S3: adding a blocking agent to the incubation device in advance through the liquid adding device 6 in the second robot arm assembly 7; transferring the sample carrier 10 on the transfer module 9 to the incubation device by the second suction cup of the third robot arm assembly 8, covering the blocking agent in an inverted manner, performing the first incubation, and then washing the sample carrier 10 for the first time; S4, a first antibody reagent is added to a clean position of the incubation device in advance by the liquid adding device 6 in the second robot arm assembly 7; the sample carrier 10 after the first cleaning is adsorbed and transferred by the second suction cup of the third robot arm assembly 8, and covered on the first antibody reagent in an inverted manner for a second incubation, and then the sample carrier 10 is cleaned for a second time; S6, the liquid adding device 6 in the second robot arm assembly 7 is used to add the amplifying agent to the clean position of the incubation device in advance; the sample carrier 10 after the first cleaning is adsorbed and transferred by the second suction cup of the third robot arm assembly 8, and covered on the amplifying agent in an inverted manner for the third incubation; the sample carrier 10 is cleaned for the third time; S7, the liquid adding device 6 in the second robot arm assembly 7 pre-adds the secondary antibody reagent to the clean position of the incubation device, and the second suction cup of the third robot arm assembly 8 absorbs and transfers the sample carrier 10 after the first washing, and covers it on the secondary antibody reagent in an inverted manner for the fourth incubation; the sample carrier 10 is washed for the fourth time; S8 adds the substrate to the clean position of the incubation device in advance through the liquid adding device 6 in the second robot arm assembly 7; the sample carrier 10 after the first cleaning is adsorbed and transferred through the second suction cup of the third robot arm assembly 8, and covered on the substrate in an inverted manner for the fifth incubation.

[0110] Example 8 The lower part of the reactor body 11 of the high-pressure dewaxing and thermal repairing device for immunohistochemistry is also provided with a base, and its periphery is also provided with a protective cover. In addition, a transfer module 9 is further provided for placing the slides after high-pressure dewaxing and thermal repair.

[0111] The operation process of the high-pressure dewaxing thermal repair device for immunohistochemistry of the present invention is as follows: 1. Device startup and preheating: The user starts the device through the touch screen interface and selects the preset dewaxing thermal repair program. The control unit starts the heating element 116 to preheat the reactor to the target temperature, for example, setting the temperature between 65°C and 75°C.

[0112] 2 Sample loading: Open the top cover 118 of the device through the corresponding button on the touch screen interface, place the glass slide containing the tissue slice on the sample carrier placement rack, start the stepper motor 15 to descend, let the top cover 118, the connecting rod 19 and the sample carrier placement rack descend together to the accommodating cavity 117 of the reactor body 11, and close the top cover 118.

[0113] 3Automatic thermal repair and dewaxing: The temperature probe 115 monitors the temperature in the accommodating chamber 117 in real time and adjusts the heating element 116 to maintain a set temperature.

[0114] 4 Cooling and removing samples: After the heat treatment is completed, the heating is stopped, and the control unit instructs the lifting mechanism 14 to gradually lift the top cover 118 through the stepper motor 15, so that the user can take out the glass slide.

[0115] 5. Equipment cleaning and shutdown: After the user removes all test samples, performs simple cleaning and maintenance, and then shuts down the device through the control unit.

[0116] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some or all of the technical features therein; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A high-pressure immunohistochemistry system, characterized in that: It comprises a thermal repair reaction device (1), an incubation device (2), and a turning mechanism (31); The thermal repair reaction device (1) and the incubation device (2) can both accommodate a sample carrier (10); The turning mechanism (31) is used to turn over and / or move the sample carrier (10).

2. The high-pressure immunohistochemistry system according to claim 1, characterized in that: The thermal repair reaction device (1) comprises: a reactor body (11), a sample carrier placement rack, a reaction drive mechanism, and a heating element (116); The reactor body (11) comprises a reactor wall and a reactor cover, wherein the reactor wall and the reactor cover form a receiving cavity (117) capable of receiving a sample carrier (10); The reactor cover is connected to a sample carrier placement rack on one side close to the accommodating cavity (117); The reactor cover is driven by a reaction drive mechanism to move closer to or away from the reactor wall; The heating element (116) is arranged on the reactor body (11).

3. The high-pressure immunohistochemistry system according to claim 2, characterized in that: The sample carrier placement rack is provided with a limiting member for limiting the vertical placement of the sample carrier (10).

4. The high-pressure immunohistochemistry system according to claim 1, characterized in that: The turning mechanism (31) comprises a rotating arm (311), a turning motor (312) and a first suction member (313). A first suction piece (313) for adsorbing the sample carrier (10) is provided at the end of the rotating arm (311), and the first suction piece (313) is used to adsorb and put down the sample carrier (10). The output shaft of the flip motor (312) is connected to the rotating shaft of the rotating arm (311), driving the rotating arm (311) to rotate.

5. The high-pressure immunohistochemistry system according to claim 1, characterized in that: It also includes an automatic sample carrier transfer device, which includes a first mechanical arm assembly (3), The first mechanical arm assembly (3) comprises a mechanical arm, a rotating arm (311) arranged on the mechanical arm and rotating between a Y direction and a Z direction, and a flip control unit, wherein the flip control unit is connected to a flip motor (312) in the flip mechanism (31) via a signal.

6. The high-pressure immunohistochemistry system according to claim 1, characterized in that: The automatic sample carrier transfer device further comprises a third mechanical arm assembly (8) and a transfer control unit, wherein the third mechanical arm assembly (8) is provided with a second absorbing member, and the second absorbing member can absorb and transfer more than one sample carrier (10) at the same time; The transfer control unit is connected to the transfer motor in the third mechanical arm assembly (8) through a signal to control the rotation of the third mechanical arm; Optionally, it also includes a transfer module (9) for accommodating more than one sample carrier (10).

7. The high-pressure immunohistochemistry system according to claim 1, characterized in that: It also includes a liquid adding device (6), wherein the liquid adding device (6) includes a liquid adding body (61) and a reagent component (62) and a cleaning component (63) arranged on the liquid adding body (61); The reagent assembly (62) comprises a liquid transfer gun (621) and a reagent driving mechanism arranged on the liquid adding body (61); The reagent driving mechanism drives the pipette gun (621) to move up and down relative to the liquid adding body (61) Z, so that the tip of the pipette gun (621) extends downward and is recovered; The cleaning component (63) comprises a liquid suction tube (631) arranged on the liquid adding body (61); An air blowing pipe (632), a liquid outlet pipe (633), and a cleaning drive mechanism; the cleaning drive mechanism drives the cleaning component (63) to move upward and downward relative to the liquid adding body (61) Z.

8. The high-pressure immunohistochemistry system according to claim 1, characterized in that: The incubation device comprises an incubation rack, which comprises a plurality of parallel incubation trays (23), each of which is connected to an adjacent incubation tray (23) via at least one supporting structure; and each of which can be independently pushed out.

9. A high pressure immunohistochemistry method, characterized in that: It includes the following steps: S1 Dewaxing and heat repairing of a sample carrier (10) carrying a sample; S2: incubating more than once, the incubation method comprising: turning over the sample carrier (10) so that the side carrying the sample covers the container with the reagent so that the reagent and the sample are attached to each other, and then incubating after adding liquid; S3 color development reaction: Add a color development reagent to the incubated sample carrier (10) to carry out a color development reaction.

10. The high pressure immunohistochemistry method according to claim 9, characterized in that: The method further comprises the following steps: in step S2, the sample carrier (10) is cleaned after incubation, and the cleaning method comprises: turning over the sample carrier (10) to expose a side carrying the sample, and cleaning with water; Optionally, dewaxing and thermal repair are performed in a thermal repair reaction device (1); The sample carrier (10) is taken out of the thermal repair reaction device (1) by means of a flipping mechanism (31), and the sample carrier (10) is flipped so that the side carrying the sample is inverted; The sample carrier (10) is moved to an incubation device via a turning mechanism (31) to perform steps including liquid addition and incubation.

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