A composite camellia oil oleogel for treating burns and scalds, its preparation method and application

Abstract

This invention discloses a composite camellia oil oleogel for treating burns and scalds, its preparation method, and its application. Using lauric acid monoglyceride as a gelling agent and antibacterial agent, and xanthan gum and sodium alginate as oleogeling agents, a composite camellia oil oleogel is prepared. The process includes the following steps: lauric acid monoglyceride is added to camellia seed oil and stirred continuously until completely dissolved; the resulting mixture is filtered through a 5-20 kDa membrane, and the filtrate is frozen overnight; a polysaccharide oleogel solution is added to the mixture of lauric acid monoglyceride and camellia oil, and the mixture is homogenized, sheared, and freeze-dried to obtain the camellia oil oleogel. This invention utilizes the above-mentioned composite camellia oil oleogel for treating burns and scalds, its preparation method, and its application. By regulating the wound microenvironment, it alleviates inflammation, increases collagen deposition, promotes angiogenesis, promotes wound healing, and reduces scar formation. The method provided by this invention has mild and simple reaction conditions and is easy to industrialize.

Description

Technical Field

[0001] This invention relates to the field of burn ointment technology, and in particular to a composite camellia oil oleogel for treating burns and scalds, its preparation method, and its application. Background Technology

[0002] Burns and scalds refer to tissue damage caused by various physical or chemical factors, including hot liquids, steam, and flames. Effective wound treatment and timely medication can quickly reduce ongoing damage and accelerate recovery; however, improper medication can not only delay recovery but also lead to scarring. Wound healing mainly involves stages of bacterial infection and inflammation, oxidative stress, cell proliferation, and tissue remodeling. It is a process of spontaneously and orderly restoring the continuity and integrity of the skin and maintaining a relatively stable internal environment. Commonly used medications include antibiotics and traditional Chinese medicines. Long-term use can increase dependence and cannot regulate the metabolic balance of collagen in the wound.

[0003] Camellia oleifera, belonging to the genus Camellia in the family Theaceae, is a unique edible oilseed tree species in my country, and is known as one of the world's four major woody oilseed plants, along with olive, oil palm, and coconut. Camellia oil is rich in various active ingredients such as tea polyphenols, tocopherols, and phytosterols, which have protective and therapeutic effects on the skin. Currently, patents CN1616052 and CN115006472A disclose a single camellia oil burn ointment, but it only prevents wound infection by containing active ingredients and isolating the wound from air, resulting in a limited efficacy in promoting wound healing. Therefore, to improve the limitations of camellia oil in wound healing, a composite camellia oil ointment with controllable morphology, high oil content, and high efficacy is being prepared to improve wound healing efficacy, showing broad application prospects in the prevention and treatment of burns and in the field of medical aesthetics. Summary of the Invention

[0004] The purpose of this invention is to provide a composite camellia oil oleogel for treating burns and scalds, its preparation method, and its application, in order to solve the above-mentioned problems.

[0005] To achieve the above objectives, this invention provides a method for preparing a composite camellia oil oleogel for treating burns and scalds. Camellia seed oil is used as the raw material, glyceryl monolaurate as a gelling agent and antibacterial agent, and xanthan gum and sodium alginate as oleogeling agents to obtain the composite camellia oil oleogel. In different phase systems, the camellia seed oil has a mass fraction of 81 wt%, the glyceryl monolaurate concentration is 7.0 wt%, the sodium alginate solution concentration is 4.8%, and the xanthan gum solution concentration is 7.2%.

[0006] The camellia seed oil contains more than 85 wt% unsaturated fatty acids.

[0007] Preferably, in the above-mentioned composite camellia oil oleogel for treating burns and scalds and its preparation method, the preparation method includes the following steps:

[0008] The lauric acid monoglyceride was added to camellia seed oil and stirred continuously until completely dissolved; the stirring speed was 3000-5000 rpm; the reaction conditions were 80℃ for 0.5 h.

[0009] The resulting mixture was filtered through a membrane with a molecular weight of 5-20 kDa, and the filtrate was frozen overnight; the freezing time was 24 h, and the freezing temperature was 4 °C.

[0010] A polysaccharide xanthan gum solution and a sodium alginate solution were prepared, and the polysaccharide xanthan gum solution and sodium alginate solution were mixed to obtain a xanthan gum-sodium alginate polysaccharide gelling agent.

[0011] A polysaccharide gelling agent solution was added to a mixture of glyceryl monolaurate and camellia oil, and the mixture was homogenized, sheared, and freeze-dried to obtain a composite camellia oil oleogel.

[0012] Preferably, in the above-mentioned composite camellia oil oleogel for treating burns and scalds and its preparation method, the xanthan gum exists in liquid form to prepare a polysaccharide xanthan gum solution. The preparation method of the polysaccharide xanthan gum solution is as follows: dissolve the polysaccharide xanthan gum in deionized water and stir magnetically to fully hydrate it to obtain a xanthan gum solution of a certain concentration, cool it to room temperature, and then refrigerate it at 4°C.

[0013] Preferably, in the above-mentioned composite camellia oil oleogel for treating burns and scalds and its preparation method, the sodium alginate is in liquid form to obtain a sodium alginate solution. The preparation method of the sodium alginate solution is as follows: dissolve sodium alginate in deionized water, heat in a water bath until completely dissolved to obtain a sodium alginate solution of a certain concentration, cool to room temperature and then refrigerate at 4°C.

[0014] Preferably, in the above-mentioned composite camellia oil oleogel for treating burns and the preparation method thereof, the method for constructing the xanthan gum-sodium alginate polysaccharide gel agent is as follows: the polysaccharide xanthan gum and sodium alginate solution are thoroughly mixed, and the pH is adjusted to 6 by adding NaOH (0.1M) and HCl (0.1M) to prepare a mixed dispersion; wherein, the concentration of sodium alginate solution is 4.8% and the concentration of xanthan gum solution is 7.2%.

[0015] Preferably, in the above-mentioned composite camellia oil oleogel for treating burns and scalds and its preparation method, the polysaccharide gelling agent solution is added to a mixture of glyceryl monolaurate and camellia oil, homogenized at 13000 rpm / min for 3 min to form a uniform mixture; after shearing at 10000 rpm / min for 3 min, it is freeze-dried to obtain the composite camellia oil oleogel.

[0016] Preferably, in the above-mentioned composite camellia oil oleogel for treating burns and its preparation method, the freeze-drying time is 48 hours and the freeze-drying temperature is -58°C.

[0017] Preferably, the composite camellia oil oleogel for treating burns obtained by the above preparation method is a composite oleogel for treating burns composed of camellia seed oil as raw material.

[0018] Preferably, the present invention provides an application of a composite camellia oil oleogel in the preparation of drugs for preventing and treating burns and repairing scars. It can prevent persistent wound infection, reduce inflammation in the body, increase collagen deposition, and promote angiogenesis, thereby promoting wound healing and reducing scar formation.

[0019] Therefore, this invention employs the aforementioned composite camellia oil oleogel for treating burns and scalds, its preparation method, and its application. Camellia seeds are cold-pressed to extract camellia seed oil, which is then combined with lauric acid monoglyceride, which possesses antibacterial and lipophilic properties, to form a camellia oil oleogel mixture. The amphiphilic nature of lauric acid monoglyceride allows its fatty acid chains to effectively embed into the oil through hydrophobic interactions. Simultaneously, the hydroxyl groups of lauric acid monoglyceride interact weakly with polar groups or ester groups in camellia oil through hydrogen bonding. This localized polar interaction helps lauric acid monoglyceride form a dense three-dimensional network structure in camellia oil, endowing it with excellent antibacterial capabilities.

[0020] Furthermore, using xanthan gum and sodium alginate as polysaccharide oleogels can effectively enhance the compactness of the three-dimensional network structure of the composite oleogels through hydrogen bonding and electrostatic interactions, significantly improving the gelling properties of the oleogels. The high adhesiveness of this composite camellia oil oleogels provides better interfacial support for cell proliferation and migration, effectively inhibits the growth of microbiota near the wound, regulates the wound microenvironment, and thus promotes wound healing by alleviating inflammation and scar formation.

[0021] A composite camellia oil ogel encapsulated with the antibacterial agent laurate monoglyceride was prepared, which is beneficial for skin penetration and wound healing. This invention overcomes the limitations of relying solely on oils, polysaccharides, and monoglycerides for skin healing, and improves upon the drawback of uncontrollable morphology. By regulating the wound microenvironment, it alleviates inflammation, increases collagen deposition, promotes angiogenesis, accelerates wound healing, and reduces scar formation. The method provided by this invention features mild and simple reaction conditions, making it suitable for industrial production.

[0022] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0023] Figure 1Macroscopic and microscopic morphology images of a composite camellia oil oleogel for treating burns and scalds according to the present invention, its preparation method and application examples 1-3;

[0024] Figure 2 The particle size distribution and oil holding capacity of the composite camellia oil oleogel for treating burns and scalds of the present invention, its preparation method and application examples 1-3 are shown in the diagram.

[0025] Figure 3 Thixotropic recovery diagrams of the composite camellia oil oleogel for treating burns and scalds of the present invention, its preparation method, and application examples 1-3;

[0026] Figure 4 The images show the antibacterial effects of the composite camellia oil oleogel for treating burns and scalds, its preparation method, and its application in Examples 1-3 and Comparative Example 1. Detailed Implementation

[0027] To better understand the above technical solutions, a detailed description of the solutions will be provided below in conjunction with the accompanying drawings and specific embodiments. Obviously, the described embodiments are merely some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0028] The terminology used in the embodiments of this invention is for the purpose of describing particular embodiments only and is not intended to limit the invention. The singular forms “a,” “the,” and “the” as used in the embodiments of this invention and the appended claims are also intended to include the plural forms, and “multiple” generally includes at least two unless the context clearly indicates otherwise.

[0029] It should also be noted that the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that an article or device that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such an article or device. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the article or device that includes said element.

[0030] Example 1

[0031] 1) Accurately weigh xanthan gum and sodium alginate, and dissolve them separately in 10g of deionized water. After they are completely dissolved, mix the fully hydrated sodium alginate solution and xanthan gum solution at a certain concentration. Finally, adjust the pH to 6 to obtain xanthan gum-sodium alginate gel. The above operation ensures that the total content of sodium alginate and xanthan gum in the system is 12wt%, of which sodium alginate is 4.8wt% and xanthan gum is 7.2wt%.

[0032] 2) The xanthan gum-sodium alginate gel system from step 1) was added to 10g of camellia oil at 80℃ and mixed thoroughly for 1h; homogenized at 10000rpm / min for 3min to form a uniform mixture, and then sheared at 10000rpm / min for 3min; then freeze-dried at -58℃ under vacuum for 48h to obtain camellia oil oleogel.

[0033] Example 2

[0034] Accurately weigh 10g of camellia seed oil and add 0.7g of lauric acid monoglyceride, stirring continuously until completely dissolved; the stirring speed is 3000-5000rpm; the reaction conditions are 80℃ for 0.5h; the resulting gel is filtered through a membrane with a molecular weight of 5-20kDa, and the filtrate is frozen for 24h at a freezing temperature of 4℃; the above operations ensure that the lauric acid monoglyceride in the system is 7wt%;

[0035] Example 3

[0036] 1) Accurately weigh xanthan gum and sodium alginate, and dissolve them separately in 10g of deionized water. After they are completely dissolved, mix the fully hydrated sodium alginate solution and xanthan gum solution at a certain concentration. Finally, adjust the pH to 6 to obtain xanthan gum-sodium alginate gel. The above operation ensures that the total content of sodium alginate and xanthan gum in the system is 12wt%, of which sodium alginate is 4.8wt% and xanthan gum is 7.2wt%.

[0037] 2) Accurately weigh 10g of camellia seed oil and add 0.7g of lauric acid monoglyceride, stirring continuously until completely dissolved; the stirring speed is 3000-5000rpm; the reaction conditions are 80℃ for 0.5h; filter the resulting gel through a membrane with a molecular weight of 5-20kDa, and freeze the filtrate for 24h at a freezing temperature of 4℃; the above operations ensure that the lauric acid monoglyceride in the system is 7wt%;

[0038] 3) The xanthan gum-sodium alginate gel obtained in step 1) was added to the gel in step 2) at 80°C and mixed thoroughly for 1 hour. After homogenization at 10,000 rpm / min for 3 minutes to form a uniform mixture, it was sheared at 10,000 rpm / min for 3 minutes. Then, it was freeze-dried at -58°C under vacuum for 48 hours to obtain the composite camellia oil oleogel.

[0039] Comparative Example 1

[0040] The preparation method of Example 1 was followed, but glyceryl monolaurate was not added in the steps, and camellia seed oil alone was used as the product obtained as Comparative Example 1.

[0041] Comparative Example 2

[0042] Jinwanhong Ointment, a commonly used burn ointment on the market, was used as a comparative example 2.

[0043] Test 1: Macroscopic and microscopic morphology of camellia oil oleogels prepared in Examples 1-3

[0044] Methods: Camellia oil oleogloss obtained in Examples 1-3 was used as the experimental sample. Its macroscopic morphology was observed and photographed in sample vials. Furthermore, its microstructure was observed using a polarizing microscope (PLM, Nikon AZ100).

[0045] result: Figure 1 The figures show the macroscopic and microscopic structures of the oleogels in Examples 1-3. As can be seen from the figures, Examples 1-2, formed after adding glyceryl monolaurate and polysaccharide gelling agents (sodium alginate and xanthan gum), all exhibit an opaque gel-like structure with a gradually increasing viscous feel. This indicates that glyceryl monolaurate and the polysaccharide oleogelling agents act as stabilizers in Examples 1-3. Furthermore, polarized light microscopy (PLM) images reveal that the microstructure of Example 1 mainly exhibits an irregular lamellar structure, while Example 2 forms rose-needle-shaped crystals with a three-dimensional network structure. Example 3, due to the inclusion of the aforementioned multi-element oleogelling agents, exhibits a coexistence of rose-needle and lamellar crystal structures. Compared to Example 2, the rose-needle structure of Example 3 is denser and more narrowly distributed. This indicates that the dispersion of polysaccharides in Example 3 facilitates the formation of cross-linked crystal branches, which further interact with the oleogel, ultimately forming a denser network structure with lower anisotropy.

[0046] Test 2: Determination of particle size and oil holding capacity of camellia oil oleogels prepared in Examples 1-3

[0047] Methods: An appropriate amount of experimental sample was taken, and its particle size was measured using a dynamic light scattering instrument (LPSA, Bettersize BT-2600E). The oil-holding capacity was determined by centrifugation. The specific steps were as follows: The oleogel sample was weighed and centrifuged at 8000 rpm for 15 min. After centrifugation, excess oil was removed, and the sample was weighed again. The formula for calculating the oil-holding capacity is as follows:

[0048]

[0049] result: Figure 2 The particle size distribution and oil holding capacity of the oleogels in Examples 1-3 are shown. All samples exhibited a multi-peaked particle size distribution, mainly concentrated between 127.3-198.5 μm. The particle size of Example 3 was significantly reduced, possibly due to the interaction between the hydrophilic groups of glyceryl monolaurate and the hydroxyl groups in the polysaccharide gelling agent. This interaction promotes the dispersion of the polysaccharide gelling agent in the oleogel and enhances the encapsulation of dispersed droplets, resulting in a smaller particle size in the composite oleogel. Furthermore, the comparison of oil holding capacity in Examples 1-3 indicates that the choice of oleogelling agent is crucial to the oil holding capacity of the composite oleogel. Anionic polysaccharide gelling agents such as xanthan gum and sodium alginate adsorb onto the oil surface through increased electrostatic deposition and steric repulsion, forming a more robust structure that encapsulates more oil molecules. In Example 3, the introduction of glyceryl monolaurate as a gelling enhancer not only strengthened the interaction between the polysaccharide gelling agent and camellia oil but also improved the density of the three-dimensional network structure between glyceryl monolaurate and camellia oil. These effects can all increase the contact surface area between the gelling agent and the oil, thereby effectively encapsulating more oil and slowing down the flow of liquid oil during centrifugation to a greater extent.

[0050] Test 3: Thixotropic recovery ability of camellia oil oleogels prepared in Examples 1-3

[0051] Methods: Thixotropic recovery testing was used to evaluate the viscosity recovery ability of camellia oil oleoglucon after shearing. The samples were first allowed to stand for 5 minutes, followed by the following steps: 300 s low shear rate (0.1 s... -1 ), 50s high shear rate (10s) -1 ) and 300s low shear rate (0.1s) -1 The viscosity recovery percentage of the oleogel was calculated by comparing the final viscosity values ​​after two low-shear rate steps.

[0052]

[0053] result: Figure 3The figures show the thixotropic recovery curves of Examples 1-3 at different shear rates. As can be seen from the figures, the apparent viscosity of the oleogel gradually decreases with increasing shear rate; however, when the shear rate recovers to a low level, the apparent viscosity recovers rapidly. This phenomenon indicates that the oleogel has good structural recovery ability. Comparing the initial viscosities of Examples 1 and 3, it was found that the polysaccharide oleogel significantly increased the initial viscosity of the oleogel, further proving that the polysaccharide gelling agent plays a dominant role in the formation of the oleogel's crystalline network. The polysaccharide oleogelling agent can increase the electrostatic repulsion of the oleogel, making its internal structure difficult to destroy. Furthermore, the addition of glyceryl monolaurate enhanced the crystallinity of Example 3, resulting in higher viscosity and elastic modulus.

[0054] Test 4: Evaluation of the antibacterial properties of the composite camellia oil oleogel prepared in Examples 1-3 and Comparative Example 1

[0055] Methods: Blank control (ultrapure water, without any added components), Comparative Example 1, and Examples 1-3 were used as experimental samples. 100 μL of each experimental sample was added to a solution containing 10% pure water in the logarithmic growth phase. 6 In a CFU / mL suspension of Escherichia coli and Staphylococcus aureus, the mixture was spread onto a solid culture medium using a sterile spreader. The culture dish was then placed in a 37°C incubator for 24 hours, and the colony growth on the culture medium was observed and recorded.

[0056] Result: From Figure 4 It can be seen that Examples 1-3 and Comparative Example 1 all have a certain inhibitory effect on Escherichia coli and Staphylococcus aureus. Compared with Comparative Example 1 and Example 1, the inhibitory effect of Examples 2-3 on bacteria is significantly enhanced. Among them, the inhibition rate of Escherichia coli in Example 3 is almost 100%. Therefore, the introduction of glyceryl monolaurate in this invention can not only act as a gel reinforcing agent to enhance the network structure of the oleogel, but also significantly improve the antibacterial activity of the oleogel. Furthermore, compared with Example 2, Example 3 has a more significant inhibitory effect on Escherichia coli and Staphylococcus aureus, which may be due to the synergistic effect of the polysaccharide gelling agent and GML in the oleogel enhancing the inhibitory effect of the oleogel on bacteria.

[0057] Test 5: Application effects of camellia oil oleogels prepared in Examples 1-3 and Comparative Examples 1-2

[0058] Methods: Forty female SD mice (20-25±5g) were selected and subjected to an acclimatization test for one week before the experiment. Mice were anesthetized by intraperitoneal injection of chloral hydrate (4%, 200mg / kg), and the hair on their backs was removed using a shaver. The wounds were then disinfected with 75% alcohol. Subsequently, a burn wound with a diameter of 8mm was created on the back of the mice using a burn probe at 100℃. The burn duration was 15s, and an external force of 500Pa was applied, successfully establishing a mouse model of third-degree burns. The wounds were then cleaned. One hour after the burn, the mice were randomly divided into 6 groups of 10 mice each: a negative control group (saline group), drug administration groups (Examples 1-3 and Comparative Example 1), and a positive control group (Comparative Example 2). The mice were housed separately and administered the drug twice daily, with each mouse receiving approximately 20mg. The condition of the burn wounds and the area of ​​the burn wounds were recorded and measured on the mornings of days 1, 4, 9, and 13. Calculate the wound healing rate using the following formula:

[0059]

[0060] Results: As shown in Table 1, compared with the negative control group, Comparative Examples 1-2 and Examples 1-3 all showed a certain promoting effect on the healing of third-degree burn wounds in mice. After 13 days of data comparison, it was found that the wound healing rate of Example 3 was significantly higher than that of the other groups, and its therapeutic effect was even comparable to the positive control (Comparative Example 2). This can be attributed to the interaction between camellia oil and various gelling agents, which accelerated the formation of new epidermis and provided a more favorable healing environment for the wound.

[0061] Table 1 Wound healing rate ( %)

[0062]

[0063]

[0064] Therefore, this invention utilizes the aforementioned composite camellia oil olegel for treating burns and scalds, along with its preparation method and application, to prepare a composite camellia oil olegel encapsulated with the antibacterial agent lauric acid monoglyceride, which is beneficial for skin penetration and wound healing. It overcomes the limitations of relying solely on oils, polysaccharides, and monoglycerides for skin healing, and improves upon the drawback of uncontrollable morphology. By regulating the wound microenvironment, it alleviates inflammation, increases collagen deposition, promotes angiogenesis, accelerates wound healing, and reduces scar formation. The method provided by this invention features mild and simple reaction conditions, facilitating industrial production. In vitro antibacterial experiments and animal experiments have demonstrated its effectiveness in promoting wound healing by reducing bacterial infection. Compared to traditional camellia oil usage, this invention introduces functional components such as lauric acid monoglyceride, significantly improving the application effect of the olegel. The preparation method of the composite camellia oil olegel of this invention is simple, highly stable, and suitable for industrial production; its application effect is significant, showing potential application prospects in burn treatment drugs. Furthermore, this invention provides a theoretical basis for the application of camellia oil in the prevention and treatment of skin diseases and in the field of medical aesthetics, and helps to improve the quality and efficiency of the camellia oil industry.

[0065] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A method for preparing a composite camellia oil oleogel for treating burns, characterized in that, A composite camellia oil oleogel was prepared using camellia seed oil as raw material, glyceryl monolaurate as a gelling agent and antibacterial agent, and xanthan gum and sodium alginate as polysaccharide oleogelizing agents. In different phase systems, the mass fraction of camellia seed oil was 81 wt%, the concentration of glyceryl monolaurate was 7.0 wt%, the concentration of sodium alginate solution was 4.8%, and the concentration of xanthan gum solution was 7.2%. Specifically, the steps include the following: Add glyceryl monolaurate to camellia seed oil and stir continuously until completely dissolved; the stirring speed is 3000-5000 rpm; the reaction conditions are 80℃ for 0.5 h. The resulting mixture was filtered through a membrane with a molecular weight of 5-20 kDa, and the filtrate was frozen overnight; the freezing time was 24 h, and the freezing temperature was 4 °C. A polysaccharide xanthan gum solution and a sodium alginate solution were prepared, and the polysaccharide xanthan gum solution and sodium alginate solution were mixed to obtain a xanthan gum-sodium alginate polysaccharide gelling agent. A polysaccharide gelling agent solution was added to a mixture of glyceryl monolaurate and camellia oil, and the mixture was homogenized, sheared, and freeze-dried to obtain a composite camellia oil oleogel.

2. The method for preparing a composite camellia oil oleogel for treating burns according to claim 1, characterized in that, Xanthan gum exists in liquid form to prepare a polysaccharide xanthan gum solution. The preparation method of the polysaccharide xanthan gum solution is as follows: dissolve polysaccharide xanthan gum in deionized water and stir magnetically to fully hydrate and obtain a xanthan gum solution of a certain concentration. After cooling to room temperature, it is refrigerated at 4°C.

3. The method for preparing a composite camellia oil oleogel for treating burns according to claim 2, characterized in that, Sodium alginate is prepared in liquid form to obtain sodium alginate solution. The preparation method of sodium alginate solution is as follows: dissolve sodium alginate in deionized water, heat in a water bath until completely dissolved, obtain sodium alginate solution of a certain concentration, cool to room temperature and then refrigerate at 4°C.

4. The method for preparing a composite camellia oil oleogel for treating burns according to claim 3, characterized in that, The method for constructing the xanthan gum-sodium alginate polysaccharide gel is as follows: the polysaccharide xanthan gum and sodium alginate solution are thoroughly mixed, and the pH is adjusted to 6 by adding 0.1M NaOH and 0.1M HCl to prepare a mixed dispersion; wherein, the concentration of sodium alginate solution is 4.8% and the concentration of xanthan gum solution is 7.2%.

5. The method for preparing a composite camellia oil oleogel for treating burns according to claim 4, characterized in that, The polysaccharide gelling agent solution was added to a mixture of glyceryl monolaurate and camellia oil, homogenized at 13,000 rpm for 3 minutes to form a uniform mixture; after shearing at 10,000 rpm for 3 minutes, it was freeze-dried to obtain the composite camellia oil oleogel.

6. The method for preparing a composite camellia oil oleogel for treating burns according to claim 5, characterized in that, The freeze-drying time was 48 hours, and the freeze-drying temperature was -58℃.

7. A composite camellia oil oleogel obtained by the preparation method according to any one of claims 1-6, characterized in that: It is a compound oil gel for treating burns and scalds, made from camellia seed oil.

8. The application of the composite camellia oil oleogel according to claim 7 in the preparation of drugs for preventing and treating burns and repairing scars.

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