Tumor organoid culture kit

By designing removable scaffolds and limiting components in the tumor organoid culture kit, the problem of inconvenient handling of the reagent bottle is solved, and the operation efficiency and safety are improved.

CN119683149AActive Publication Date: 2025-03-25SHANDONG FUYOU LIFE SCI CO LTD
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Patent Information

Application Number
CN202510201118.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-24
Publication Date
2025-03-25
Estimated Expiration
2045-02-24

AI Technical Summary

Technical Problem

During the process of tumor organoid culture, the reagent bottle is inconvenient to hold and hold it incorrectly or knock it down, which increases the difficulty and cost of operation.

Method used

A tumor organoid culture kit is designed, including removable scaffolds and limiting components, through which the reagent bottles and markers are limited and fixed, improving the convenience and safety of operation.

Benefits of technology

Through the design of scaffolds and limiting components, it is more convenient for operators to hold and place reagent bottles, which improves the efficiency of tumor organoid culture and ensures the safety of reagent bottles.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention provides a tumor organoid culture kit, and belongs to the technical field of kits. Comprising a kit body, a kit cover is rotationally connected to one side of the kit body, a support is inserted into the kit body, reagent bottles and a label plate are placed on the support, and a batten is inserted into the label plate; the limiting assembly is used for limiting the reagent bottles and the label plate, and the limiting assembly is respectively connected with the bracket and the box body. By arranging the limiting assembly and the support, the reagent bottles and the label plates can be placed, the support and the limiting assembly are taken out of the kit body according to the using process of the reagent bottles and the label plates, then the reagent bottles and the label plates are stored and limited independently, and after the support and the limiting assembly are taken out of the kit body, the reagent bottles and the label plates are stored and limited independently. An operator can take and place the reagent bottle more conveniently, the efficiency of culturing the tumor organoids is improved, and the safety of the reagent bottle in the repeated use process is ensured.
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Description

Technical Field

[0001] The present invention relates to the technical field of kits, and in particular to a tumor organoid culture kit. Background Art

[0002] The test kit is a box used to hold chemical reagents for detecting chemical components, drug residues, virus types, etc. A tumor organoid culture kit is needed for tumor organoid culture. The tumor organoid culture kit generally stores sample cleaning solution, culture medium, primary clearing solution, stop solution, organoid digestion solution, cell culture plates and other related reagents and tools. In the test kit, plastic sponges are currently used to limit the reagent bottles, and disposable sealing bags are used to seal the cell culture plates.

[0003] When culturing tumor organoids, it is necessary to take out the reagent bottle from the reagent kit and place it on the experimental table, and then disassemble the cell culture plate from the disposable sealed bag and take it out. During the entire culturing process, the operator needs to repeatedly add reagents to the cell culture plate. Since there are many types of reagents and the reagent bottles look similar, it is easy to take the wrong bottle or knock it over if the reagent bottle is directly placed on the table. Since the friction of the plastic sponge in the reagent kit is large, it is inconvenient for the operator to directly use the reagent kit to conveniently and efficiently take and place the reagent bottle. This requires the operator to use other auxiliary tools to temporarily store and limit the reagent bottle, which increases the cost of tumor organoid culture. Therefore, the present invention provides a tumor organoid culture kit to meet the needs. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a tumor organoid culture kit which can not only realize the placement of reagent bottles and label plates by setting a limiting component and a bracket, but also take out the bracket and the limiting component from the box body according to the use process of the reagent bottles and label plates, and then store and limit the reagent bottles and label plates separately. When the bracket and the limiting component are taken out from the box body, it is not only more convenient for operators to take and place reagent bottles, but also improves the efficiency of tumor organoid culture and ensures the safety of reagent bottles during repeated use. The above settings can solve the problem of inconvenient taking and placing of reagent bottles during use.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions: A tumor organoid culture kit comprises a box body, one side of which is rotatably connected to a box cover, a bracket inserted in the box body, a reagent bottle and a label plate placed on the bracket, and a slat inserted on the label plate; a limiting component is used to limit the reagent bottle and the label plate, and the limiting component is respectively connected to the bracket and the box body.

[0006] Optionally, the limiting assembly includes a storage tube and a first storage plate fixedly connected to the bottom of the bracket, a second storage plate is fixedly connected to the bottom of the first storage plate, and an extrusion block is fixedly connected to the inner wall of the box body.

[0007] Optionally, the storage tube is a cylinder structure with a cylindrical profile, a lightweight groove is opened on the storage tube, and the storage tube and the bracket are an integral injection-molded structure.

[0008] Optionally, the position of the extrusion block corresponds to the position of the storage tube, the top of the extrusion block is fixedly connected with an extrusion sheet, the extrusion sheet is inserted into the lightweight slot, and the middle position of the extrusion sheet has an inwardly concave arc.

[0009] Optionally, the size of the first storage plate is greater than that of the second storage plate, and the first storage plate, the second storage plate and the bracket are an integral injection-molded structure.

[0010] Optionally, a first positioning block is fixedly connected to the top of the first storage plate, an inserting block is fixedly connected to the bottom of the target plate, and a groove body matching the outline of the inserting block is provided at the middle position of the first positioning block.

[0011] Optionally, a second positioning block is fixedly connected to the top of the second storage plate, the overall contour of the second positioning block is a sphere, and the top of the second positioning block is an open segmented structure.

[0012] Optionally, an arc-shaped piece is fixedly connected to the bottom of the bracket, one end of the arc-shaped piece is fixedly connected to a support portion, and a limiting portion is fixed on an outer wall of the bottom of the arc-shaped piece.

[0013] Optionally, the arc-shaped piece has an inwardly inclined angle, a middle position of the arc-shaped piece has an inwardly recessed arc-shaped profile, and the supporting portion and the limiting portion are both curved arc-shaped structures.

[0014] Optionally, an inclined surface and a limit block are fixedly connected to the inner wall of the box body, the inclined surface has an angle inclined toward the middle position of the box body, a groove with a "U"-shaped profile is opened in the middle position of the support part, and the position of the limit block corresponds to the position of the "U"-shaped profile groove in the middle of the support part.

[0015] Compared with the prior art, the present invention has at least the following beneficial effects: In the above scheme, compared with the prior art, the technical scheme provided by the present application can not only realize the placement of reagent bottles and label plates, but also take out the bracket and the limit assembly from the box body according to the use process of the reagent bottles and label plates, and then store and limit the reagent bottles and label plates separately. When the bracket and the limit assembly are taken out of the box body, it is not only more convenient for the operator to take and place the reagent bottles, but also improves the efficiency of tumor organoid culture, ensures the safety of the reagent bottles during repeated use, and improves the practical application value of the test kit.

[0016] By providing structures such as arc pieces, supporting parts and limiting parts, not only can the bracket be more stable during transportation, but the impact force and collision force generated during transportation can also be buffered and absorbed, thereby improving the safety of the reagent bottles during transportation. Moreover, the bracket can be removed from the box body and placed on the table separately, making it more convenient and efficient for users to take and place reagent bottles during tumor organ culture. The cooperation between the arc pieces and the supporting parts makes the bracket more stable when the user places it on the table, ensuring that the bracket will not tip over or fall over when the user takes and places the reagent bottles, thereby improving the safety of the reagent bottles.

[0017] By providing a storage tube and an extrusion block, when the bracket is placed in the box body, the storage tube can form a structural cooperation with the extrusion block in the box body. The effect achieved by the cooperation makes the reagent bottle more firm and stable in the storage tube, thereby ensuring that the reagent bottle will not sway or shake in the storage tube during transportation, thereby improving the safety of the reagent bottle. When the bracket is taken out of the box body, the storage tube can only simply position the reagent bottle without squeezing and limiting it, thereby making it easier and more convenient for the user to take and put the reagent bottle.

[0018] By setting the first placement plate and the second placement plate, the label plate and the slats can be limited and fixed respectively. This not only improves the efficiency of users in taking and placing the label plate and the slats, but also effectively ensures the safety and convenience of the label plate and the slats during secondary use, thereby making the entire tumor organoid culture process simpler and more efficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings, which are incorporated herein and constitute a part of the specification, illustrate embodiments of the invention and, together with the description, further serve to explain the principles of the invention and to enable those skilled in the relevant art to make and use the invention.

[0020] Figure 1 This is a first-person perspective diagram of the three-dimensional structure of the tumor organoid culture kit; Figure 2 This is a schematic diagram of the third-dimensional structure of the tumor organoid culture kit from the second perspective; Figure 3 It is a schematic diagram of the enlarged three-dimensional structure of the box body and the box cover; Figure 4 It is a schematic diagram of the enlarged three-dimensional structure of the limit assembly; Figure 5 It is a schematic diagram of the three-dimensional structure of the box body and the limit assembly in a cutaway first-person perspective; Figure 6 It is a schematic diagram of the three-dimensional structure of the box body and the limiting component in a cutaway second viewing angle; Figure 7 It is a schematic diagram of the three-dimensional structure of the limit assembly in a cutaway first-person perspective; Figure 8 It is a schematic diagram of the three-dimensional structure of the limit assembly in a cutaway second viewing angle; Fig. 9 It is a schematic diagram of the enlarged three-dimensional structure of the extrusion block; Fig.10 It is a schematic diagram of an enlarged three-dimensional structure of the first storage plate and the second storage plate; Fig.11 This is a schematic diagram of the three-dimensional structure of the target plate when viewed from above; Fig.12 It is a top view of the three-dimensional structure of the second storage plate and the slats.

[0021] Reference numerals: 1. Box body; 101. Box cover; 102. Inclined surface; 103. Limiting block; 2. Bracket; 201. Arc-shaped piece; 202. Supporting part; 203. Limiting part; 3. Storage tube; 4. First storage plate; 401. First positioning block; 402. Second storage plate; 403. Second positioning block; 5. Extrusion block; 501. Extrusion sheet; 6. Reagent bottle; 7. Marking plate; 701. Slat; 702. Insert block.

[0022] As shown in the figure, in order to clearly implement the structure of the embodiment of the present invention, specific structures and devices are marked in the figure, but this is only for illustrative purposes and is not intended to limit the present invention to the specific structure, device and environment. According to specific needs, ordinary technicians in this field can adjust or modify these devices and environments. DETAILED DESCRIPTION

[0023] The following is a detailed description of a tumor organoid culture kit provided by the present invention in conjunction with the accompanying drawings and specific embodiments. At the same time, it is explained here that in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments, and those skilled in the art may also adopt other alternatives to implement some known technologies; and the accompanying drawings are only for a more specific description of the embodiments, and are not intended to specifically limit the present invention.

[0024] It should be noted that the references to "one embodiment", "embodiment", "exemplary embodiments", "some embodiments" and the like in the specification indicate that the embodiments described may include specific features, structures or characteristics, but not every embodiment may include the specific features, structures or characteristics. In addition, when a specific feature, structure or characteristic is described in conjunction with an embodiment, it should be within the knowledge of a person skilled in the art to implement such feature, structure or characteristic in conjunction with other embodiments (whether or not explicitly described).

[0025] In general, a term can be understood, at least in part, from its use in context. For example, depending, at least in part, on the context, the term "one or more" as used herein can be used to describe any feature, structure, or characteristic in the singular sense, or can be used to describe a combination of features, structures, or characteristics in the plural sense. Additionally, the term "based on" can be understood as not necessarily intended to convey an exclusive set of factors, but can instead, depending, at least in part, on the context, allow for the presence of other factors that are not necessarily explicitly described.

[0026] It will be understood that the meanings of “on,” “over,” and “above” in the present invention should be interpreted in the broadest manner, so that “on” not only means “directly on” something, but also includes the meaning of being “on” something with intervening features or layers therebetween, and “on” or “over” not only means “on” or “above” something, but also includes the meaning of being “on” or “above” something with no intervening features or layers therebetween.

[0027] Additionally, spatially relative terms such as "under," "beneath," "lower," "above," "upper," and the like may be used herein for descriptive convenience to describe the relationship of one element or feature to another element or features, as shown in the accompanying drawings. Spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the accompanying drawings. The device may be oriented in other ways, and the spatially relative descriptors used herein may be similarly interpreted accordingly.

[0028] like Figures 1 to 5As shown, an embodiment of the present invention provides a tumor organoid culture kit, including a box body 1, a box cover 101 is rotatably connected to one side of the box body 1, a bracket 2 is inserted in the box body 1, a reagent bottle 6 and a mark plate 7 are placed on the bracket 2, and a strip 701 is inserted on the mark plate 7; a limiting component is used to limit the reagent bottle 6 and the mark plate 7, and the limiting component is connected to the bracket 2 and the box body 1 respectively. The box body 1 and the box cover 101 are both hard paper shells. The existing tumor organoid culture kit usually places a whole piece of plastic sponge in the box body 1, and then opens a placement hole on the plastic sponge. By placing the reagent bottle 6 in the placement hole on the plastic sponge, friction is used to achieve positioning. The limiting effect of the reagent bottle 6 is achieved by storing the label plate 7 in a disposable sealed bag. The present application uses the bracket 2 and the limiting component to limit the reagent bottle 6 and the label plate 7 at the same time, without the need to place them separately. Compared with the prior art, the technical solution provided by the present application can not only realize the placement of the reagent bottle 6 and the label plate 7, but also take out the bracket 2 and the limiting component from the box body 1 according to the use process of the reagent bottle 6 and the label plate 7, and then store and limit the reagent bottle 6 and the label plate 7 separately. After the bracket 2 and the limiting component are taken out from the box body 1, it is not only more convenient for the operator to take and place the reagent bottle 6, but also improves the efficiency of tumor organoid culture and improves the practical application value of the test kit.

[0029] In this embodiment, if Figures 1 to 9As shown, the bottom of the bracket 2 is fixedly connected with an arc-shaped piece 201, one end of the arc-shaped piece 201 is fixedly connected with a support portion 202, a limiting portion 203 is fixed on the bottom outer wall of the arc-shaped piece 201, the arc-shaped piece 201 has an inwardly inclined angle, the middle position of the arc-shaped piece 201 has an inwardly concave arc profile, the support portion 202 and the limiting portion 203 are both curved arc structures, the inner wall of the box body 1 is fixedly connected with an inclined surface 102 and a limiting block 103, the inclined surface 102 has an angle inclined toward the middle position of the box body 1, a "U"-shaped groove is provided in the middle position of the support portion 202, and the limiting block The position of 103 corresponds to the position of the "U"-shaped contour groove in the middle of the support part 202. The bracket 2 is a rectangular sheet structure made of plastic material. Such material selection can save cost investment. Four arc-shaped pieces 201 extend from the bottom side of the bracket 2. The four arc-shaped pieces 201 are respectively located on the four side lines of the rectangular contour of the bracket 2. Since the arc-shaped pieces 201 have an inwardly inclined angle and the inclined surface 102 on the inner wall of the box body 1 also has an angle inclined toward the middle position of the box body 1, when the user needs to place the bracket 2 in the box body 1, the bracket 2 can be pressed into the box body 1 from top to bottom, and the bracket 2 can be placed in the box body 1. During the pressing process, the arc-shaped piece 201 and the inclined surface 102 squeeze each other, and the two are assisted by the inclined surface 102 for guiding and limiting, thereby ensuring that the bracket 2 will not be misplaced during the pressing process, and improving the efficiency of the user in assembling the bracket 2. Furthermore, since the position of the limit block 103 corresponds to the position of the "U"-shaped contour groove in the middle of the support part 202, when the user presses down the bracket 2, the limit block 103 will slide in the "U"-shaped contour groove in the middle of the support part 202, and the bracket 2 is limited by the adaptation effect of the contours between the two, ensuring that the bracket 2 is pressed down and used. There will be no displacement in the box body 1 during the process, which improves the stability of the bracket 2 and makes the reagent bottle 6 more stable and safe during transportation. Furthermore, since the middle position of the arc piece 201 has an inwardly concave arc profile, the middle position of the arc piece 201 is more easily deformed under the extrusion of external force under the action of the arc profile. This arrangement ensures that if the reagent bottle 6 is hit or bumped during transportation, the middle position of the arc piece 201 will be deformed, and the impact force and bump force will be buffered and absorbed while deforming, thereby improving the safety of the reagent bottle 6.

[0030] When culturing tumor organoids, the bracket 2 can be pulled upward to deform the limiting plate and disengage from the limiting block 103. After the bracket 2 is taken out of the box body 1, it is placed on the desktop. Since the support portion 202 and the limiting portion 203 are both curved arc structures and are arranged on both sides of the arc-shaped piece 201, when the user places the bracket 2 on the desktop, the support portion 202 and the limiting portion 203 can simultaneously support and limit the bracket 2. The two can increase the contact area between the bottom of the bracket 2 and the desktop, thereby improving the overall stability of the bracket 2. Secondly, since the arc-shaped piece 201 has an inwardly inclined angle and cooperates with the arc profile of the upper limit portion 203, the arc-shaped piece 201, the limiting portion 203 and the desktop form a triangular profile support system, thereby effectively improving the stability of the bracket 2 after it is placed on the desktop. The stability of the structure ensures that the bracket 2 will not tip over or fall over when the user takes or places the reagent bottle 6; in summary, the coordination of the arc piece 201, the support portion 202, the limit portion 203 and other structures not only makes the bracket 2 more stable during transportation, but also can buffer and absorb the impact force and collision force generated during transportation, thereby improving the safety of the reagent bottle 6 during transportation, and the bracket 2 can be removed from the box body 1 and placed on the desktop separately, thereby making it more convenient and efficient for the user to take or place the reagent bottle 6 during the tumor organoid culture process. In addition, the coordination of the arc piece 201 and the support portion 202 ensures that the bracket 2 will have better stability when the user places the reagent bottle 6 on the desktop, ensuring that the bracket 2 will not tip over or fall over when the user takes or places the reagent bottle 6, thereby improving the safety of the reagent bottle 6.

[0031] As an implementation method in this embodiment, Figures 4 to 9As shown, the limiting assembly includes a storage barrel 3 and a first storage plate 4 fixedly connected to the bottom of the bracket 2, the bottom of the first storage plate 4 is fixedly connected to the second storage plate 402, and an extrusion block 5 is fixedly connected to the inner wall of the box body 1. The storage barrel 3 is a cylinder structure with a cylindrical profile, and a lightweight groove is opened on the storage barrel 3. The storage barrel 3 and the bracket 2 are an integral injection-molded structure. The position of the extrusion block 5 corresponds to the position of the storage barrel 3. The top of the extrusion block 5 is fixedly connected to an extrusion sheet 501, which is inserted into the lightweight groove. The middle position of the extrusion sheet 501 has an inwardly concave arc. In the limiting assembly, the reagent bottle 6 is mainly inserted into the lightweight groove through the storage barrel 3. The holder 2 is provided with a plurality of locking members 100, 102 and 104 locking members 106. The holder 2 is provided with a plurality of locking members 100, 104 and 104 locking members 107. The holder 2 is provided with a plurality of locking members 100, 102 and 104 locking members 108. The holder 2 is provided with a plurality of locking members 100, 104 and 104 locking members 106. The middle position of the squeezing sheet 501 has an inwardly concave arc, so the concave arc in the middle of the squeezing sheet 501 can squeeze the reagent bottle 6 in the storage barrel 3, thereby improving the firmness of the reagent bottle 6 in the storage barrel 3, so that the reagent bottle 6 will not sway in the storage barrel 3 during transportation. Since the squeezing block 5 is fixed to the bottom of the box body 1, when the bracket 2 is taken out of the box body 1, the squeezing sheet 501 will not continue to squeeze the reagent bottle 6 in the storage barrel 3, and the user will be more relaxed when taking and placing the reagent bottle 6. In summary, when the bracket 2 is placed in the box body 1, the storage barrel 3 can be squeezed with the squeezing block 5 in the box body 1. The pressing block 5 forms a structural fit, and the effect achieved by the fit makes the reagent bottle 6 more firm and stable in the storage barrel 3, thereby ensuring that the reagent bottle 6 will not sway or shake in the storage barrel 3 during transportation, thereby improving the safety of the reagent bottle 6. When the bracket 2 is taken out of the box body 1, the storage barrel 3 can only simply position the reagent bottle 6 without squeezing or limiting it, thereby making it easier and more convenient for the user to take and place the reagent bottle 6; since the storage barrel 3 and the bracket 2 are an integral injection-molded structure, during the production process, the storage rack and the bracket 2 are integrally injection-molded using the same mold, making the two easier to produce and manufacture, reducing capital investment.

[0032] Optionally, relevant information of the reagent bottle 6 is posted at the position of the storage tube 3 on the bracket 2, so that the user can refer to the information on the bracket 2 when taking or placing the reagent bottle 6, ensuring that the reagent bottle 6 will not be taken or placed incorrectly. Since the bracket 2 is made of plastic, the user can also choose to record relevant information of the reagent bottle 6 on the bracket 2 with a signature pen.

[0033] In this embodiment, if Figures 4 to 12 As shown, the size of the first storage plate 4 is larger than that of the second storage plate 402, the first storage plate 4, the second storage plate 402 and the bracket 2 are an integral injection-molded structure, the top of the first storage plate 4 is fixedly connected with a first positioning block 401, the bottom of the mark plate 7 is fixedly connected with an insert block 702, the middle position of the first positioning block 401 is provided with a groove body adapted to the contour of the insert block 702, the top of the second storage plate 402 is fixedly connected with a second positioning block 403, the overall contour of the second positioning block 403 is a sphere, and the top of the second positioning block 403 is an open segmented structure, in the limiting assembly, the mark plate 7 is mainly fixed by the first storage plate 4 and the second storage plate 402, and the bracket 2, the first storage plate 4 and the second storage plate 402 are fixed from top to bottom. The one-piece structure connected to each other below is integrally injection molded by a mold during actual production. Since the sizes of the first storage plate 4 and the mark plate 7 are consistent, the user can directly place the mark plate 7 on the top of the first storage plate 4 from top to bottom. Because a groove body adapted to the contour of the plug block 702 is provided at the middle position of the first positioning block 401, when the user places the mark plate 7 on the top of the first storage plate 4, the plug block 702 will be directly plugged into the groove body at the middle position of the first positioning block 401, and the mark plate 7 is limited and fixed with the help of the first positioning block 401. Optionally, a transparent plastic sealing sticker is used to seal the mark plate 7 at the position of the first storage plate 4 on the top of the bracket 2 to ensure that the mark plate 7 will not be contaminated by the external environment in the box body 1.

[0034] Before the operator carries out tumor organoid culture, he needs to determine the number of slats 701 on the mark plate 7 to be used, and then remove the excess slats 701 from the mark plate 7 and re-place them in a disposable sealed bag for storage. This operation has the following disadvantages: when the slats 701 are removed, they become messy and inconvenient to put into the sealed bag, and it is inconvenient to take and put them when they are used again. In addition, when the scattered slats 701 are carried in the box body 1, they will collide with each other due to shaking, which is not only easy to cause damage but also make noise; in the present application, when using After the user removes the mark plate 7 from the first storage plate 4, the user can place the extra slats 701 on the second storage plate 402. The second storage plate 402 is provided with a second positioning block 403. Since the overall contour of the second positioning block 403 is a sphere and the top of the second positioning block 403 is an open segmented structure, the top of the second positioning block 403 is elastic. When the user places the slats 701 on the second storage plate 402, the slats 701 can be inserted into the gap between two adjacent second positioning blocks 403 (such as Fig.12As shown in the figure, during the insertion of the slat 701, the top of the second positioning block 403 will be squeezed, and the top of the second positioning block 403 will be deformed and contracted inwardly. During the deformation of the second positioning block 403, the slat 701 will be reversely squeezed, thereby achieving the fixing effect of the slat 701. Furthermore, when the label plate 7 is used up, it can be replaced on the top of the first storage plate 4. Since the gap between the first storage plate 4 and the second storage plate 402 is greater than the height of the slat 701, placing the slat 701 on the second storage plate 402 will not affect the storage of the label plate 7. After the two are placed, they can be sealed again with the transparent plastic seal on the top of the bracket 2. The label plate 7 and the slats 701 are sealed with paper, and the next time the label plate 7 and the slats 701 are used, they can be directly taken from the first storage plate 4 and the second storage plate 402, and under the limiting action of the second positioning block 403, the slats 701 will not collide with each other, and it is convenient and efficient to take the slats 701; in summary, by setting the first storage plate 4 and the second storage plate 402, the label plate 7 and the slats 701 can be limited and fixed respectively, which on the one hand improves the efficiency of users taking and placing the label plate 7 and the slats 701, and on the other hand effectively ensures the safety and convenience of the label plate 7 and the slats 701 during the secondary use, thereby making the entire tumor organoid culture process more concise and efficient.

[0035] The working principle of the technical solution provided by the present invention is as follows: When the user needs to perform tumor organoid culture operation, the box cover 101 on the top of the box body 1 is opened, and then the bracket 2 is pulled upward to deform the limiting plate and disengage the limiting block 103. When the bracket 2 is taken out of the box body 1, it is placed on the desktop. Since the support portion 202 and the limiting portion 203 are both curved arc structures and both are arranged on both sides of the arc-shaped piece 201, when the user places the bracket 2 on the desktop, the support portion 202 and the limiting portion 203 can simultaneously support and limit the bracket 2. The two can increase the contact area between the bottom of the bracket 2 and the desktop, thereby improving the overall stability of the bracket 2. Secondly, since the arc-shaped piece 201 has an inwardly inclined angle and cooperates with the arc contour of the upper limit portion 203, the arc-shaped piece 201, the limiting portion 203 and the desktop form a triangular contour support system, thereby effectively improving the stability of the structure after the bracket 2 is placed on the desktop. The urging member 501 is preferably positioned at the bottom of the box body 1 so that the urging member 501 is not loosened or tilted when the urging member 5 is in the box body 1.

[0036] When the user needs to use the label plate 7, the user tears off one end of the transparent plastic sealing sticker on the top of the first storage plate 4 and then takes the label plate 7 out of the first storage plate 4 from bottom to top. After the user takes the label plate 7 off the first storage plate 4, the excess slats 701 are placed on the second storage plate 402. Since the overall contour of the second positioning block 403 is a sphere and the top of the second positioning block 403 is an open segmented structure, the top of the second positioning block 403 is elastic. When the user places the slat 701 on the second storage plate 402, the slat 701 can be inserted into the gap between two adjacent second positioning blocks 403. During the insertion process of the slat 701, the top of the second positioning block 403 will be squeezed, and the top of the second positioning block 403 will be deformed and contracted inwardly. During the deformation process of the second positioning block 403, the slat 701 will be reversely squeezed, thereby achieving the fixing effect of the slat 701. After use, the plate 7 can be replaced on the top of the first storage plate 4, because a groove body that matches the contour of the insertion block 702 is opened in the middle position of the first positioning block 401. Therefore, when the user places the mark plate 7 on the top of the first storage plate 4, the insertion block 702 will be directly inserted into the groove body in the middle position of the first positioning block 401, and the mark plate 7 is limited and fixed with the help of the first positioning block 401. Since the gap between the first storage plate 4 and the second storage plate 402 is greater than the height of the slat 701, the slat 701 placed on the second storage plate 402 will not affect the storage of the mark plate 7. After the two are placed, they can be sealed again with the help of the transparent plastic sealing sticker on the top of the bracket 2. The next time the mark plate 7 and the slat 701 are used, they can be directly taken from the first storage plate 4 and the second storage plate 402, and under the limiting action of the second positioning block 403, the slats 701 will not collide with each other, and it is convenient and efficient to take the slat 701.

[0037] The present invention covers any substitution, modification, equivalent method and scheme made on the essence and scope of the present invention. In order to make the public have a thorough understanding of the present invention, specific details are described in detail in the following preferred embodiments of the present invention, but those skilled in the art can fully understand the present invention without the description of these details. In addition, in order to avoid unnecessary confusion about the essence of the present invention, well-known methods, processes, procedures, components and circuits are not described in detail.

[0038] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A tumor organoid culture kit, comprising a box body, characterized in that: A box cover is rotatably connected to one side of the box body, a bracket is inserted into the box body, a reagent bottle and a label are placed on the bracket, and a strip is inserted into the label; A limiting component is used to limit the reagent bottle and the mark plate, and the limiting component is connected to the bracket and the box body respectively.

2. The tumor organoid culture kit according to claim 1, characterized in that: The limiting assembly includes a storage tube and a first storage plate fixedly connected to the bottom of the bracket, a second storage plate is fixedly connected to the bottom of the first storage plate, and an extrusion block is fixedly connected to the inner wall of the box body.

3. The tumor organoid culture kit according to claim 2, characterized in that: The storage tube is a cylinder structure with a cylindrical profile, a lightweight groove is opened on the storage tube, and the storage tube and the bracket are an integral injection-molded structure.

4. The tumor organoid culture kit according to claim 3, characterized in that: The position of the extrusion block corresponds to the position of the storage tube. The top of the extrusion block is fixedly connected with an extrusion sheet, which is inserted into the lightweight slot. The middle position of the extrusion sheet has an inwardly concave arc.

5. The tumor organoid culture kit according to claim 2, characterized in that: The size of the first storage plate is greater than that of the second storage plate, and the first storage plate, the second storage plate and the bracket are an integral injection-molded structure.

6. The tumor organoid culture kit according to claim 2, characterized in that: A first positioning block is fixedly connected to the top of the first storage plate, an inserting block is fixedly connected to the bottom of the mark plate, and a groove body matching the outline of the inserting block is provided at the middle position of the first positioning block.

7. The tumor organoid culture kit according to claim 2, characterized in that: A second positioning block is fixedly connected to the top of the second storage plate. The overall outline of the second positioning block is a sphere, and the top of the second positioning block is an open segmented structure.

8. The tumor organoid culture kit according to claim 2, characterized in that: An arc-shaped piece is fixedly connected to the bottom of the bracket, one end of the arc-shaped piece is fixedly connected to a support portion, and a limiting portion is fixed on the outer wall of the bottom of the arc-shaped piece.

9. The tumor organoid culture kit according to claim 8, characterized in that: The arc-shaped piece has an inwardly inclined angle, the middle position of the arc-shaped piece has an inwardly concave arc-shaped contour, and the supporting portion and the limiting portion are both curved arc-shaped structures.

10. The tumor organoid culture kit according to claim 8, characterized in that: An inclined surface and a limit block are fixedly connected to the inner wall of the box body, the inclined surface has an angle inclined toward the middle position of the box body, a groove with a "U"-shaped profile is opened in the middle position of the support part, and the position of the limit block corresponds to the position of the "U"-shaped groove in the middle of the support part.

Citation Information

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