Targeted liver cancer exosome preparation equipment based on coarse precipitation

Abstract

The exosome preparation equipment comprises a control box, a centrifugal assembly and an extraction assembly, the centrifugal assembly comprises a centrifugal seat, a rotating shaft and a rotating seat, and the extraction assembly comprises a main pipe body, an overflow pipe and a filter pipe. Through cooperation of the centrifugal assembly and the extraction assembly, extraction of the exosome can be completed without taking a test tube out of a centrifugal machine during preparation of the exosome, so that the situation that the exosome is re-dispersed due to oscillation of the test tube is avoided, automatic extraction of the exosome is achieved, the preparation efficiency of the exosome is effectively improved, and the production cost is reduced. In addition, the main pipe body is rotationally arranged on the rotating seat, so that the main pipe body can be in an inclined state before centrifugation, the centrifugation effect is further improved, a filter membrane assembly is arranged in the filter pipe, large-particle impurities in the exosome can be further filtered through a filter membrane, and the purity of the exosome is further improved.

Description

Technical Field

[0001] The present invention relates to the technical field of exosome preparation, and in particular to a device for preparing exosomes targeting liver cancer based on crude precipitation. Background Art

[0002] In recent years, with the continuous development of biomedical technology, exosomes have gradually shown their unique advantages as a new type of drug carrier in cancer treatment. Exosomes have good biocompatibility and low cytotoxicity, can carry a variety of drugs and accurately target cancer cells, thereby improving the treatment effect. Especially in the treatment of liver cancer, drug-loaded exosomes targeting liver cancer have attracted much attention.

[0003] At present, exosomes need to be crudely precipitated during the preparation process, that is, in the initial stage of exosome extraction, large particles are precipitated by low-speed or medium-speed centrifugation to obtain a relatively clear supernatant, which is convenient for the subsequent extraction of exosomes. The crude precipitation method is simple and fast, and is widely used. In this process, a centrifuge is generally required, that is, the test tube is placed in the centrifuge for centrifugation, and the test tube is taken out after centrifugation, and then the exosomes are extracted manually, which makes the extraction efficiency of exosomes relatively low. In addition, the test tube is often shaken in the process of manually taking out the test tube, which causes the exosomes to disperse, further affecting the extraction work.

[0004] Therefore, it is necessary to provide a crude precipitation-based exosome preparation device targeting liver cancer to solve the problems raised in the above background technology. Summary of the invention

[0005] To achieve the above object, the present invention provides the following technical solution: a crude precipitation-based exosome preparation device targeting liver cancer, comprising a control box, a centrifugal component and an extraction component, wherein the centrifugal component is rotatably arranged in the control box, and four extraction components are rotatably arranged on the centrifugal component;

[0006] The centrifugal assembly comprises a centrifugal seat, a rotating shaft and a rotating seat, wherein a rotating shaft is fixedly arranged at the bottom of the centrifugal seat, four rotating seats are fixedly arranged on the centrifugal seat in a circumferential manner, a driving motor for driving the rotating shaft to rotate is fixedly arranged at the bottom of the control box, two supporting rods are fixedly arranged on the rotating seat, and an extraction assembly is rotatably arranged between the two supporting rods;

[0007] The extraction assembly comprises a main body, an overflow pipe and a filter pipe, wherein the main body is rotatably arranged between the two support rods, an overflow pipe is slidably arranged in the main body, a filter pipe is fixedly arranged in the main body, and the filter pipe is sealed and slidably connected with the overflow pipe, the overflow pipe and the filter pipe divide the interior of the main body into an overflow chamber and a filter chamber, an arc tray is fixedly arranged on the top of the filter pipe, and a filter membrane assembly is also rotatably arranged in the filter pipe;

[0008] The filter membrane assembly comprises a filter seat and a filter membrane, wherein the filter seat is rotatably arranged on the filter tube, and the filter membrane is fixedly arranged on the filter seat.

[0009] Further, as a preference, both ends of the main pipe body are arranged in an arc shape and an arc plate having the same diameter as the arc surface is fixedly arranged on the top of the main pipe body;

[0010] The rotating seat is C-shaped, and arc grooves are arranged on the top and the bottom of the rotating seat, and the main pipe rotates in a sealed manner along the arc grooves.

[0011] Further, as a preference, a gear ring is embedded on one side of the arc plate close to the rotating seat, a rack is slidably provided on the rotating seat, the rack is meshed with the gear ring, a hydraulic rod is fixedly provided on the rack, an annular hydraulic cavity is opened in the centrifugal seat, a hydraulic ring is sealingly and slidably provided in the hydraulic cavity, and the hydraulic ring is fixedly connected to the hydraulic rod.

[0012] Further, as a preference, an injection channel is obliquely opened on the top of the rotating seat, the injection channel is communicated with the input end of the main pipe body, and a conical material delivery port is fixedly provided at the input end of the main pipe body;

[0013] The control box is provided with an injection port on the top, the centrifugal seat is provided with a cache cavity on the top, a hydraulic cavity 2 is provided at the bottom of the cache cavity, a push plate is provided in the cache cavity for sealing and sliding, a push rod is fixed on the push plate, and the push rod slides along the hydraulic cavity 2 for sealing, the cache cavity is connected with the injection port, and the top of the cache cavity is connected with the input ends of the four injection channels.

[0014] Further, as a preference, a discharge pipe 1 is provided at the bottom of the arc-shaped tray, a solenoid valve 1 is provided on the discharge pipe 1, a discharge pipe 2 is provided at the bottom of the filter tube, a solenoid valve 2 is provided on the discharge pipe 2, a through hole 1 and a through hole 2 are provided at the bottom of the main tube body, the through hole 1 is connected to the overflow chamber, the through hole 2 is sealed and connected to the discharge pipe 2, and a liquid level gauge is also provided on the top of the overflow pipe.

[0015] Further, as a preference, the rotating seat is provided with through hole three and through hole four respectively connected with the through hole one and through hole two, and two annular grooves are fixedly provided at the bottom of the rotating seat, and the two annular grooves respectively connect the plurality of through holes three and through holes four;

[0016] Two sealing grooves are arranged at the bottom of the control box, and the two annular grooves are respectively rotated along the two sealing grooves in a sealed manner. Two discharge pipes are arranged on the control box, and the two discharge pipes are respectively communicated with the two sealing grooves.

[0017] Further, as a preference, a plurality of guide rods are fixedly arranged on the overflow pipe, and a second hydraulic rod is vertically fixedly arranged on the guide rods;

[0018] The main pipe body is provided with a plurality of guide grooves, a hydraulic chamber three is provided in the guide grooves, the output end of the guide rod is inserted into the guide grooves and slides along the guide grooves, and the hydraulic rod two slides along the hydraulic chamber three in a sealed manner.

[0019] Further, as a preference, two rotating blocks are fixedly provided on the filter seat, one of which is fixedly provided with a gear, a rack 2 is slidably provided in the filter tube, a hydraulic chamber 4 is opened in the filter tube, a hydraulic rod 3 is fixedly provided on the rack 2, the hydraulic rod 3 slides sealingly along the hydraulic chamber 4, and the rack 2 is meshed with the gear.

[0020] Furthermore, preferably, a control unit and a control panel are provided on the control box.

[0021] Compared with the prior art, the present invention provides a device for preparing exosomes targeting liver cancer based on crude precipitation, which has the following beneficial effects:

[0022] In the present invention, by providing a centrifugal component and an extraction component and cooperating with the two, the extraction of exosomes can be completed without taking the test tube out of the centrifuge during the preparation of exosomes, thereby avoiding the redispersion of exosomes due to the vibration of the test tube, and thereby realizing the automatic extraction of exosomes, effectively improving the preparation efficiency of exosomes. In addition, the main body is rotatably arranged on a rotating seat, so that the main body can be in an inclined state before centrifugation, thereby further improving the centrifugal effect, and a filter membrane component is arranged in the filter tube, and large particle impurities in the exosomes can be further filtered through the filter membrane, thereby improving the purity of the exosomes. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the overall process of the present invention;

[0024] Figure 2 It is a schematic diagram of the overall structure of the present invention;

[0025] Figure 3It is a schematic cross-sectional view of the overall structure of the present invention;

[0026] Figure 4 It is a structural schematic diagram of the centrifugal assembly in the present invention;

[0027] Figure 5 for Figure 4 A schematic diagram of the enlarged structure of the middle part A;

[0028] Figure 6 It is a structural schematic diagram of the extraction component in the present invention;

[0029] Figure 7 for Figure 6 A magnified schematic diagram of the structure of the middle B section;

[0030] In the figure: 1. control box; 11. injection port; 12. discharge pipe; 13. control panel; 2. centrifugal assembly; 21. centrifugal seat; 211. hydraulic ring; 212. buffer chamber; 213. push plate; 22. rotating shaft; 23. rotating seat; 231. rack one; 232. hydraulic rod one; 233. injection channel; 234. ring groove; 24. support rod; 3. extraction assembly; 31. main body; 311. arc plate; 312. gear ring; 313. through hole one; 314. through hole two; 32. overflow pipe; 321. guide rod; 322. hydraulic rod two; 33. filter tube; 331. rack two; 332. hydraulic chamber four; 34. arc tray; 35. filter membrane assembly; 351. filter seat; 352. filter membrane; 353. rotating block; 354. gear. DETAILED DESCRIPTION

[0031] See also Figure 1 to Figure 7 In an embodiment of the present invention, a crude precipitation-based exosome preparation device targeting liver cancer includes a control box 1, a centrifugal component 2 and an extraction component 3, wherein the centrifugal component 2 is rotatably arranged in the control box 1, and four extraction components 3 are rotatably arranged on the centrifugal component 2;

[0032] The centrifugal assembly 2 includes a centrifugal seat 21, a rotating shaft 22 and a rotating seat 23, wherein the rotating shaft 22 is fixedly arranged at the bottom of the centrifugal seat 21, and four rotating seats 23 are fixedly arranged on the centrifugal seat 21 in a circumferential manner, and a driving motor for driving the rotating shaft 22 to rotate is fixedly arranged at the bottom of the control box 1, and two supporting rods 24 are fixedly arranged on the rotating seat 23, and an extraction assembly 3 is rotatably arranged between the two supporting rods 24;

[0033] The extraction assembly 3 includes a main body 31, an overflow pipe 32 and a filter pipe 33, wherein the main body 31 is rotatably arranged between the two support rods 24, the overflow pipe 32 is slidably arranged in the main body 31, the filter pipe 33 is fixedly arranged in the main body 31, and the filter pipe 33 is sealed and slidably connected with the overflow pipe 32, the overflow pipe 32 and the filter pipe 33 divide the interior of the main body 31 into an overflow chamber and a filter chamber, an arc tray 34 is fixedly arranged on the top of the filter pipe 33, and a filter membrane assembly 35 is also rotatably arranged in the filter pipe 33;

[0034] The filter membrane assembly 35 includes a filter seat 351 and a filter membrane 352, wherein the filter seat 351 is rotatably disposed on the filter tube 33, and the filter membrane 352 is fixedly disposed on the filter seat 351;

[0035] A gear ring 312 is embedded on one side of the arc plate 311 close to the rotating seat 23, a rack 231 is slidably provided on the rotating seat 23, the rack 231 is meshed with the gear ring 312, a hydraulic rod 232 is fixedly provided on the rack 231, an annular hydraulic chamber 1 is provided in the centrifugal seat 21, a hydraulic ring 211 is sealingly and slidably provided in the hydraulic chamber 1, and the hydraulic ring 211 is fixedly connected to the hydraulic rod 1 232;

[0036] The top of the rotating seat 23 is inclined to open an injection channel 233, the injection channel 233 is connected to the input end of the main pipe body 31, and the input end of the main pipe body 31 is fixedly provided with a conical feeding port 312;

[0037] The control box 1 is provided with an injection port 11 at the top, the centrifugal seat 21 is provided with a cache chamber 212 at the top, a hydraulic chamber 2 is provided at the bottom of the cache chamber 212, a push plate 213 is provided in a sealing and sliding manner in the cache chamber 212, a push rod is fixedly provided on the push plate 213, and the push rod slides in a sealing manner along the hydraulic chamber 2, the cache chamber 212 is communicated with the injection port 11, and the top of the cache chamber 212 is communicated with the input ends of the four injection channels 233;

[0038] In particular, the buffer chamber 212 is provided so that the injected exosome solution can enter the buffer chamber 212 first, and then the exosome solution overflows into the four main bodies 31 under the pushing of the push plate 212, so that the amount of solution entering each main body 31 is equal through overflow, thereby preventing the excess or insufficient solution in a main body 31 due to biased flow, thereby affecting the centrifugal effect and the extraction of exosomes.

[0039] A discharge pipe 1 is provided at the bottom of the arc tray 34, and a solenoid valve 1 is provided on the discharge pipe 1. A discharge pipe 2 is provided at the bottom of the filter tube 33, and a solenoid valve 2 is provided on the discharge pipe 2. A through hole 1 313 and a through hole 2 314 are provided at the bottom of the main pipe body 31, and the through hole 1 313 is connected to the overflow chamber, and the through hole 2 314 is sealed and connected to the discharge pipe 2, and a liquid level gauge is also provided on the top of the overflow pipe 32;

[0040] A plurality of guide rods 321 are fixedly disposed on the overflow pipe 32, and a hydraulic rod 322 is vertically fixedly disposed on the guide rods 321;

[0041] The main body 31 is provided with a plurality of guide grooves, in which a hydraulic chamber 3 is provided, the output end of the guide rod 321 is inserted into the guide groove and slides along the guide groove, and the hydraulic rod 2 322 slides along the hydraulic chamber 3 in a sealed manner;

[0042] The rotating seat 23 is provided with a through hole 3 and a through hole 4 which are connected to the through hole 1 313 and the through hole 2 314 respectively. Two annular grooves 234 are fixedly provided at the bottom of the rotating seat 23. The two annular grooves 234 respectively connect the plurality of through holes 3 and 4.

[0043] The control box 1 has two sealing grooves at the bottom, and the two annular grooves 234 rotate along the two sealing grooves in a sealed manner. The control box 1 is provided with two discharge pipes 12, and the two discharge pipes 12 are connected to the two sealing grooves respectively.

[0044] In particular, the annular groove 234 and the sealing groove are provided so that no matter where the centrifugal seat 21 rotates to, the through hole three and the through hole four are always connected to the two sealing grooves, thereby effectively discharging the supernatant, exosomes and cleaning liquid from the control box 1. Similarly, a sealing cavity structure with the same structure as the sealing groove and the annular groove 234 can be provided between the control box 1 and the centrifugal seat 21, and the hydraulic pressure is transmitted through the sealing cavity. A plurality of hydraulic transmission channels are provided in the centrifugal seat 21 and the rotating seat 23, thereby providing hydraulic pressure for the sliding of the hydraulic ring 211, the rack 1 231, the overflow pipe 32 and the push plate 213, and the transmission of this hydraulic pressure is not affected by the rotation of the centrifugal seat 21;

[0045] Two rotating blocks 353 are fixedly arranged on the filter seat 351, one of which is fixedly arranged with a gear 354, a rack 2 331 is slidably arranged in the filter tube 33, a hydraulic chamber 4 332 is opened in the filter tube 33, a hydraulic rod 3 is fixedly arranged on the rack 2 331, the hydraulic rod 3 slides along the hydraulic chamber 4 332 in a sealed manner, and the rack 2 331 is meshed with the gear 354;

[0046] The control box 1 is provided with a control unit and a control panel 13 .

[0047] During implementation, the injection port 11 on the control box 1 is opened, and the exosome solution is injected into the cache chamber 212 from the injection port 11. At this time, the push plate 213 is located at the bottom of the cache chamber 212. After the cache chamber 212 is filled with the solution, the push plate 213 is driven to slide upward, so that the solution in the cache chamber 212 overflows and flows to the four injection channels 233, and flows into the main body 31 from the injection channels 233. In this process, the overflowing exosome solution can be evenly diverted to the four extraction components 3, so that the amount of solution in the four extraction components 3 is the same. Then, the solution entering the main body 31 flows into the overflow pipe 32 and the filter tube 33 through the conical feed port 312. At this time, the electromagnetic Valve 1 and solenoid valve 2 are both in a closed state, and then the control unit is controlled by the control panel 13 to drive the hydraulic ring 211 to slide along the hydraulic chamber 1, thereby causing the rack 1 231 to slide downward, and the sliding of the rack 1 231 causes the gear ring 312 to rotate, thereby driving the main body 31 to rotate along the arc groove seal, so that the main body 31 is in an inclined state. During this process, the arc plate 311 can slide along the arc groove seal, and then the injection channel 233 is blocked after the main body 31 is deflected, and then the drive motor is started by the control panel 13 to rotate the centrifugal seat 21, thereby driving the four extraction components 3 to rotate, that is, the exosome solution is centrifuged. And set a specific time as needed. After the centrifugation is completed, the main body tube 31 is reset, and then the overflow pipe 32 is driven to slide downward, so that the supernatant in the overflow pipe 32 flows into the overflow cavity and is discharged through the through hole 1 313 set at the bottom of the main body 31 to the sealing groove connected with the through hole 3 at the bottom of the control box 1, and is discharged through the discharge pipe 12. During this process, the liquid level in the overflow pipe 32 is monitored by a liquid level gauge. When the supernatant is completely overflowed, the overflow pipe 32 is reset, and then the solenoid valve 1 is opened, so that the sediment on the arc tray 34 is discharged from the discharge pipe 1 to the filter membrane assembly 35, and large particles of impurities are filtered out by the filter membrane 352, and pure exosomes pass through the filter membrane 352 into the The bottom of the filter tube 33 is opened, and then the solenoid valve 2 is opened, so that the exosomes flow from the discharge pipe 2 to the sealing groove connected to the through hole 4, and then the exosomes are discharged and collected through the discharge pipe 12. When the exosomes are extracted and collected, the hydraulic rod 3 is driven to slide along the hydraulic chamber 4 332, so that the rack 2 331 drives the gear 354 to rotate, so that the filter seat 351 drives the filter membrane 352 to flip, so that the filter surface of the filter membrane 352 faces downward, and then the cleaning program is started through the control panel 13, and the cleaning liquid is injected from the injection port 11, and then the cleaning liquid enters the cache chamber 212, the injection channel 233, the main body 31, the overflow pipe 32 and the filter tube 33 in sequence to clean them.Then, the electromagnetic valve 1 and the electromagnetic valve 2 are opened, so that the cleaning liquid flushes the filter membrane 352, thereby cleaning the impurities on the filter membrane 352, and then the cleaning liquid is discharged from the two feeding pipes 12 after cleaning, and then the rotating seat 23 is driven to rotate to dry the cleaning liquid on the main body 31, the overflow pipe 32, the filter pipe 33 and the filter membrane 352, and hot air can be blown into the discharge pipe 12 to completely dry the cleaning liquid, and then the various components are reset to facilitate the next exosome extraction work, thereby realizing the automatic extraction of exosomes and effectively improving the exosome preparation efficiency.

[0048] In this embodiment, Figure 6 and 7 The two ends of the main pipe body 31 are arranged in an arc shape and an arc plate 311 having the same diameter as the arc surface is fixedly arranged on the top of the main pipe body 31;

[0049] The rotating seat 23 is C-shaped, and arc grooves are disposed on the top and bottom of the rotating seat 23 . The main pipe 31 rotates in a sealed manner along the arc grooves.

[0050] In summary, in the implementation of the present invention, by setting the centrifugal component 2 and the extraction component 3 and cooperating with the two, the exosomes can be extracted without taking the test tube out of the centrifuge during preparation, thereby avoiding the redispersion of the exosomes due to the oscillation of the test tube, and thereby realizing the automatic extraction of the exosomes, effectively improving the preparation efficiency of the exosomes. In addition, the main body 31 is rotatably set on the rotating seat 23, so that the main body 31 can be in an inclined state before centrifugation, thereby further improving the centrifugal effect, and a filter membrane component 35 is set in the filter tube 33, and the large particle impurities in the exosomes can be further filtered through the filter membrane 352, thereby improving the purity of the exosomes.

[0051] What is described above is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A device for preparing exosomes targeting liver cancer based on crude precipitation, characterized in that: It comprises a control box (1), a centrifugal assembly (2) and an extraction assembly (3), wherein the centrifugal assembly (2) is rotatably arranged in the control box (1), and four extraction assemblies (3) are rotatably arranged on the centrifugal assembly (2); The centrifugal assembly (2) comprises a centrifugal seat (21), a rotating shaft (22) and a rotating seat (23), wherein the rotating shaft (22) is fixedly arranged at the bottom of the centrifugal seat (21), four rotating seats (23) are fixedly arranged in a circle on the centrifugal seat (21), a driving motor for driving the rotating shaft (22) to rotate is fixedly arranged at the bottom of the control box (1), two supporting rods (24) are fixedly arranged on the rotating seat (23), and an extraction assembly (3) is rotatably arranged between the two supporting rods (24); The extraction assembly (3) comprises a main body (31), an overflow pipe (32) and a filter pipe (33), wherein the main body (31) is rotatably arranged between the two support rods (24), an overflow pipe (32) is slidably arranged in the main body (31), a filter pipe (33) is fixedly arranged in the main body (31), and the filter pipe (33) is sealed and slidably connected to the overflow pipe (32), the overflow pipe (32) and the filter pipe (33) divide the interior of the main body (31) into an overflow chamber and a filter chamber, an arc-shaped tray (34) is fixedly arranged on the top of the filter pipe (33), and a filter membrane assembly (35) is also rotatably arranged in the filter pipe (33); The filter membrane assembly (35) comprises a filter seat (351) and a filter membrane (352), wherein the filter seat (351) is rotatably arranged on the filter tube (33), and the filter membrane (352) is fixedly arranged on the filter seat (351).

2. According to claim 1, a crude precipitation-based exosome preparation device targeting liver cancer is characterized by: Both ends of the main pipe (31) are arranged in an arc shape, and an arc-shaped plate (311) having the same diameter as the arc-shaped surface is fixedly arranged on the top of the main pipe (31); The rotating seat (23) is C-shaped, and arc-shaped grooves are provided at the top and the bottom of the rotating seat (23), and the main pipe (31) rotates in a sealed manner along the arc-shaped grooves.

3. The device for preparing exosomes targeting liver cancer based on crude precipitation according to claim 2, characterized in that: A gear ring (312) is embedded in a side of the arc plate (311) close to the rotating seat (23); a rack (231) is slidably provided on the rotating seat (23); the rack (231) is meshed with the gear ring (312); a hydraulic rod (232) is fixedly provided on the rack (231); an annular hydraulic chamber (21) is provided in the centrifugal seat (21); a hydraulic ring (211) is sealingly and slidably provided in the hydraulic chamber (21); the hydraulic ring (211) is fixedly connected to the hydraulic rod (232).

4. The device for preparing exosomes targeting liver cancer based on crude precipitation according to claim 1, characterized in that: An injection channel (233) is obliquely provided on the top of the rotating seat (23), the injection channel (233) is communicated with the input end of the main pipe body (31), and a conical material delivery port (312) is fixedly provided on the input end of the main pipe body (31); The control box (1) is provided with an injection port (11) at the top, the centrifugal seat (21) is provided with a buffer chamber (212) at the top, a second hydraulic chamber is provided at the bottom of the buffer chamber (212), a push plate (213) is provided in a sealing and sliding manner in the buffer chamber (212), a push rod is fixedly provided on the push plate (213), the push rod slides in a sealing manner along the second hydraulic chamber, the buffer chamber (212) is communicated with the injection port (11), and the top of the buffer chamber (212) is communicated with the input ends of the four injection channels (233).

5. The device for preparing exosomes targeting liver cancer based on crude precipitation according to claim 1, characterized in that: A discharge pipe 1 is provided at the bottom of the arc-shaped tray (34), and a solenoid valve 1 is provided on the discharge pipe 1; a discharge pipe 2 is provided at the bottom of the filter tube (33), and a solenoid valve 2 is provided on the discharge pipe 2; a through hole 1 (313) and a through hole 2 (314) are provided at the bottom of the main pipe body (31); the through hole 1 (313) is connected to the overflow chamber, and the through hole 2 (314) is connected to the discharge pipe 2 in a sealed manner; and a liquid level gauge is also provided at the top of the overflow pipe (32).

6. The device for preparing exosomes targeting liver cancer based on crude precipitation according to claim 5, characterized in that: The rotating seat (23) is provided with a through hole three and a through hole four respectively connected to the through hole one (313) and the through hole two (314); two annular grooves (234) are fixedly provided at the bottom of the rotating seat (23); the two annular grooves (234) respectively connect the plurality of through holes three and through holes four; The control box (1) has two sealing grooves at the bottom, and the two annular grooves (234) respectively rotate along the two sealing grooves in a sealed manner. The control box (1) is provided with two discharge pipes (12), and the two discharge pipes (12) are respectively connected to the two sealing grooves.

7. The device for preparing exosomes targeting liver cancer based on crude precipitation according to claim 1, characterized in that: A plurality of guide rods (321) are fixedly arranged on the overflow pipe (32), and a second hydraulic rod (322) is vertically fixedly arranged on the guide rods (321); The main pipe body (31) is provided with a plurality of guide grooves, a hydraulic chamber three is provided in the guide grooves, the output end of the guide rod (321) is inserted into the guide grooves and slides along the guide grooves, and the hydraulic rod two (322) slides sealingly along the hydraulic chamber three.

8. The device for preparing exosomes targeting liver cancer based on crude precipitation according to claim 1, characterized in that: Two rotating blocks (353) are fixedly arranged on the filter seat (351), one of the rotating blocks (353) being fixedly arranged with a gear (354), a rack (331) being slidably arranged in the filter tube (33), a hydraulic chamber (332) being provided in the filter tube (33), a hydraulic rod (3) being fixedly arranged on the rack (331), the hydraulic rod (3) slidingly sliding along the hydraulic chamber (332), and the rack (331) being meshed with the gear (354).

9. The device for preparing exosomes targeting liver cancer based on crude precipitation according to claim 1, characterized in that: The control box (1) is provided with a control unit and a control panel (13).

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