Accurate sampling device for detecting tumor cell pleuroperitoneal metastasis

Abstract

The invention provides a sampling device for tumor cell pleuroperitoneal metastasis detection. The sampling device comprises a liquid storage container, a sampling assembly, a sampling assembly and an electromagnetic stirring assembly. The electromagnetic stirring assembly comprises an electromagnetic stirrer arranged on the outer wall of the bottom of the liquid storage container and a magnetic core arranged on the inner wall of the bottom of the liquid storage container, and magnetic field force generated when the electromagnetic stirring assembly works can drive the sample liquid in the liquid storage container to be uniformly stirred. The sampling assembly and the sampling assembly are each provided with a control system (namely a flow controller and an electromagnetic valve), and when the preset capacity is reached, the flow controller can control the electromagnetic valve to be automatically closed. According to the invention, the sample liquid is fully and uniformly stirred by the electromagnetic stirrer and is accurately sub-packaged in the plurality of sampling tubes, so that the accuracy of the detection result of the tumor cell pleuroperitoneal metastasis and the processing efficiency are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of medical devices, and in particular relates to a sampling device for detecting thoracic and peritoneal metastasis of tumor cells. Background Art

[0002] Tumor cell peritoneal metastasis is currently a common tumor metastasis pathway, especially in abdominal organ malignancies such as gastric cancer and colorectal cancer. The incidence of tumor cell peritoneal metastasis is relatively high, seriously affecting the patient's survival prognosis. Most tumor cell peritoneal metastasis occurs through direct spread and lymphatic transfer. Early tumor cell peritoneal metastasis is difficult to screen out through imaging tests and ctDNA (circulating tumor DNA) blood tests.

[0003] At present, cytological examination of peritoneal lavage fluid can be used as the gold standard for diagnosing free tumor cells in the abdominal cavity. The main operation is to flush the abdominal cavity and peritoneum with lavage fluid. The tumor cells in the abdominal cavity will fall off into the lavage fluid under the flushing of the lavage fluid. The lavage fluid is then extracted and the thoracic and abdominal lavage fluid is used as a test sample for cytological examination or ctDNA (circulating tumor DNA) detection based on the lavage fluid. This allows for early screening of tumor cell metastasis and timely adoption of relevant medical measures.

[0004] For the pleural and abdominal lavage fluid obtained, there is currently no mature pleural and peritoneal tumor cell collection device. The lavage fluid is mainly introduced into the storage device for storage by manual operation, or introduced into the suction ball by pressing the suction ball to form negative pressure for storage, and then manually divided into multiple sampling test tubes for tumor cell metastasis detection. However, in actual applications, it is found that many factors will affect the accuracy of tumor cell pleural and peritoneal metastasis detection results, mainly: 1) If the storage container is placed for too long, it will cause cell precipitation in the lavage fluid and uneven distribution of tumor cells in the sample fluid, thereby affecting the content of tumor cells in the final sampling, resulting in deviations in the test results; 2) Human error, different operation time, operation techniques, and operation strength will lead to inconsistent sample collection, too much or too little. These factors will reduce the accuracy of the test, resulting in the possibility of false negative rate in the test results, delaying the patient's precious treatment time. At the same time, due to the inconsistent amount of tumor cells in different sampling test tubes, inconsistent test results will occur, affecting the reliability of the test results and causing diagnostic doubts. Summary of the invention

[0005] The present invention aims at the problems of the prior art and provides a sampling device for detecting pleural and peritoneal metastasis of tumor cells in order to improve the accuracy and reliability of the detection results.

[0006] In order to achieve the above technical objectives, the present invention adopts the following technical solutions:

[0007] A sampling device for detecting pleural and peritoneal metastasis of tumor cells, comprising: a liquid storage container, a sampling component, a sampling component, an electromagnetic stirring component, a negative pressure suction device, and a sample liquid suction device;

[0008] The liquid storage container is used to store the sample liquid, and is provided with a liquid inlet of the liquid storage container and a negative pressure suction port of the liquid storage container at the top, a liquid outlet of the liquid storage container is provided at the bottom, and a negative pressure suction device is connected to the negative pressure suction port of the liquid storage container;

[0009] The sampling assembly includes a sampling liquid inlet and a first connecting tube, one end of the first connecting tube is connected to the sampling liquid inlet, and the other end is connected to the liquid inlet of the liquid storage container, and the sample liquid suction device is connected to the sampling liquid inlet;

[0010] The sampling assembly includes a sampling liquid outlet and a second connecting tube, one end of the second connecting tube is connected to the liquid outlet of the liquid storage container, and the other end is connected to the sampling liquid outlet;

[0011] The electromagnetic stirring assembly comprises: an electromagnetic stirrer arranged on the outer wall of the bottom of the liquid storage container, and a magnetic core arranged on the inner wall of the bottom of the liquid storage container;

[0012] The sampling component and the sampling component are respectively provided with a sampling control switch and a sampling control switch.

[0013] Preferably, a first flow controller and a first solenoid valve are sequentially arranged on the first connecting pipe of the sampling assembly according to the liquid flow direction;

[0014] The first flow controller is provided with a control system, the input end of the control system is connected to the signal output end of the first flow controller, and the output end of the control system is connected to the first solenoid valve.

[0015] Preferably, the first flow controller and the first solenoid valve are both connected to the power supply device.

[0016] Preferably, the first flow controller and the first connecting pipe, and the first solenoid valve and the first connecting pipe are all sealed.

[0017] Preferably, a second flow controller and a second solenoid valve are sequentially arranged on the second connecting pipe of the sampling assembly according to the liquid flow direction;

[0018] The second flow controller is provided with a control system, the input end of the control system is connected to the signal output end of the second flow controller, and the output end of the control system is connected to the second solenoid valve.

[0019] Preferably, the second flow controller and the second solenoid valve are both connected to the power supply device.

[0020] Preferably, the second flow controller and the second connecting pipe, as well as the second solenoid valve and the second connecting pipe are all sealed.

[0021] Preferably, the electromagnetic stirring assembly is also provided with a stirring control switch, and the electromagnetic stirrer is connected to a power supply device.

[0022] Preferably, the liquid storage container is made of any one of glass and medical polymer materials.

[0023] Preferably, the first connecting tube and the second connecting tube are made of medical polymer materials.

[0024] Compared with the prior art, the beneficial effects of the present invention are:

[0025] 1) By introducing electromagnetic stirring technology, various substances in the peritoneal lavage fluid in the storage container can be quickly and thoroughly mixed to ensure the uniformity of metastatic cells that may exist in the ascites, and to ensure the uniformity and consistency of the sample. Compared with traditional stirring methods, electromagnetic stirring is uniform and gentle, and can better protect the integrity of cells, reduce the risk of cell rupture and damage caused by excessive or uneven stirring during sample processing, and lead to deviations in tumor cell and ctDNA detection.

[0026] 2) Through the two sets of control components (flow controller and solenoid valve) for sampling and sampling in and out, the amount of sample collected into the liquid storage container and the amount of sample taken out of the sampling tube are accurately controlled to avoid the influence of too much or too little collection on the test results, and ultimately ensure the consistency of the sampling amount.

[0027] 3) Automatic control replaces manual operation. The storage, retrieval and stirring processes of the lavage fluid are all automatically controlled, which greatly improves the sample processing efficiency. The sampling, storage and sampling operations of the lavage fluid are standardized, which minimizes the operation time and human errors in the detection, and provides a reliable, efficient and standardized sampling device.

[0028] In summary, the present invention accurately dispenses the lavage fluid drawn from the peritoneal cavity into multiple sampling tubes after being fully and evenly stirred, thereby avoiding the influence of factors such as sample liquid precipitation and human operation on the detection accuracy, ensuring the uniformity and consistency of the sample, and improving the accuracy and reliability of the detection of peritoneal metastasis of tumor cells. At the same time, the device is simple to operate, the process is standardized, and the sample processing efficiency is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 A longitudinal section diagram of an embodiment of the present invention Figure 1 ;

[0030] Figure 2 A longitudinal section diagram of an embodiment of the present invention Figure 2 ;

[0031] Figure 3 for Figure 2 Schematic diagram of the transverse section at A in the middle;

[0032] Figure 4 for Figure 2 Schematic diagram of the transverse section at B in the middle;

[0033] Figure 5 for Figure 2 Schematic diagram of the transverse section at C in the middle;

[0034] Figure 6 for Figure 2 Schematic diagram of the transverse section at D in the middle;

[0035] In the accompanying drawings: 1. sampling component; 2. liquid storage container; 3. electromagnetic stirring component; 4. sampling component; 5. electromagnetic stirrer; 6. magnetic core; 7. liquid inlet of liquid storage container; 8. negative pressure suction port of liquid storage container; 9. liquid outlet of liquid storage container; 10. sampling liquid inlet; 11. first flow controller; 12. first solenoid valve 1; 13. first connecting pipe; 14. second flow controller; 15. second solenoid valve; 16. second connecting pipe; 17. sampling liquid outlet; 18. stirring control switch; 19. sampling control switch; 20. sampling control switch. DETAILED DESCRIPTION

[0036] The following will be combined with the attached embodiment of the present invention Figures 1 to 6 , the technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] In the description of the present invention, it should be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "the other end", "upper", "one side", "top", "inside", "front", "center", "both ends" and the like indicate positions or positional relationships based on the attached Figures 1 to 5 The orientation or positional relationship shown is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0038] In the present invention, unless otherwise clearly stipulated and limited, the terms such as "installation", "setting", "connection", "fixation" and "screw-on" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be the internal connection of two elements or the interaction relationship between two elements. Unless otherwise clearly defined, ordinary technicians in this field can understand the specific meanings of the above terms in the present invention according to the specific circumstances.

[0039] Example 1

[0040] The present invention provides a sampling device for detecting pleural and peritoneal metastasis of tumor cells, the device comprising: a liquid storage container, a sampling component, a sampling component, an electromagnetic stirring component, a negative pressure suction device, and a sample liquid suction device;

[0041] The liquid storage container is used to store the sample liquid, and is provided with a liquid inlet of the liquid storage container and a negative pressure suction port of the liquid storage container at the top, a liquid outlet of the liquid storage container is provided at the bottom, and a negative pressure suction device is connected to the negative pressure suction port of the liquid storage container;

[0042] The sampling assembly includes a sampling liquid inlet and a first connecting tube, one end of the first connecting tube is connected to the sampling liquid inlet, and the other end is connected to the liquid inlet of the liquid storage container, and the sample liquid suction device is connected to the sampling liquid inlet;

[0043] The sampling assembly includes a sampling liquid outlet and a second connecting tube, one end of the second connecting tube is connected to the liquid outlet of the liquid storage container, and the other end is connected to the sampling liquid outlet;

[0044] The electromagnetic stirring assembly comprises: an electromagnetic stirrer arranged on the outer wall of the bottom of the liquid storage container, and a magnetic core arranged on the inner wall of the bottom of the liquid storage container;

[0045] The sampling assembly and the sampling assembly are respectively provided with a sampling control switch and a sampling control switch. Conventional valves and medical clamps can be used to control sampling and the start and stop of sampling.

[0046] During sampling, the sample liquid drawn by the sample liquid suction device enters from the sampling liquid inlet, flows through the first connecting tube into the liquid inlet of the liquid storage container, and is stored in the liquid storage container; during sampling, the sample liquid flows from the liquid storage container outlet through the second connecting tube, flows out from the sampling liquid outlet, and finally is injected into the external sampling test tube. During stirring, the electromagnetic stirring component generates a magnetic field force to drive the liquid in the liquid storage container to stir evenly.

[0047] Example 2

[0048] like Figures 1 to 6As shown, an embodiment of the present invention provides a sampling device for detecting pleural and peritoneal metastasis of tumor cells. The device needs to be connected to an external negative pressure suction device and a sample liquid suction device for use. The sampling device as a whole includes: a liquid storage container 2, a sampling component 1, a sampling component 4, an electromagnetic stirring component 3, a negative pressure suction device, and a sample liquid suction device.

[0049] The liquid storage container 2 in the middle is used to store the sample liquid. The upper part is provided with a liquid storage container liquid inlet 7 and a liquid storage container negative pressure suction port 8. The bottom part is provided with a liquid storage container liquid outlet 9. The negative pressure suction device is connected to the liquid storage container negative pressure suction port 8.

[0050] The sampling assembly 1 above it includes a sampling liquid inlet 10 and a first connecting tube 13. One end of the first connecting tube 13 is connected to the sampling liquid inlet 10, and the other end is connected to the liquid inlet 7 of the liquid storage container. The sample liquid suction device is connected to the sampling liquid inlet 10.

[0051] The sampling assembly 4 below it includes a sampling liquid outlet 17 and a second connecting tube 16. One end of the second connecting tube 16 is connected to the liquid outlet 9 of the liquid storage container, and the other end is connected to the sampling liquid outlet 17.

[0052] The electromagnetic stirring assembly 3 in the middle includes: an electromagnetic stirrer 5 arranged on the outer wall of the bottom of the liquid storage container, and a magnetic core 6 arranged on the inner wall of the bottom of the liquid storage container;

[0053] The sampling component and the sampling component are respectively provided with a sampling control switch and a sampling control switch.

[0054] A first flow controller 11 and a first solenoid valve 12 are sequentially arranged on the first connecting pipe 13 of the sampling assembly 1 according to the liquid flow direction;

[0055] The first flow controller 11 is provided with a control system, the input end of the control system is connected to the first flow controller 11, and the output end of the control system is connected to the first solenoid valve 12;

[0056] When the sample liquid injected into the liquid storage container 2 reaches a preset capacity, the first flow controller 11 controls the first electromagnetic valve 12 to close and stop injecting the sample liquid.

[0057] A second flow controller 14 and a second solenoid valve 15 are sequentially arranged on the second connecting pipe 16 of the sampling assembly 4 according to the liquid flow direction;

[0058] The second flow controller 14 is provided with a control system, the input end of the control system is connected to the second flow controller 14, and the output end of the control system is connected to the second solenoid valve 15;

[0059] When the sample liquid injected into the external sampling tube reaches a preset capacity, the second flow controller 14 controls the second electromagnetic valve 15 to close and stop injecting the sample liquid.

[0060] The electromagnetic stirring assembly 3 is also provided with a stirring control switch 18, which can manually start or shut down the electromagnetic stirrer 5.

[0061] The magnetic core 6 can be in different shapes, including disc, rod, cross, etc.

[0062] The liquid storage container 2 can be made of different materials, preferably a material with good penetration into the magnetic field, such as glass or medical polymer material.

[0063] The sampling control switch 20 provided in the sampling assembly 1, in this embodiment, specifically controls the opening or closing of the first flow controller 11. The first flow controller 11 will synchronously control the opening and closing of the first solenoid valve 12, thereby controlling the start or stop of sampling.

[0064] The sampling control switch 19 provided in the sampling assembly 4 is specifically the opening or closing of the second flow controller 14 in this embodiment. The second flow controller 14 will synchronously control the opening and closing of the second electromagnetic valve 15, thereby controlling the start or stop of sampling.

[0065] The electromagnetic stirrer 5, the first flow controller 11, the first electromagnetic valve 12, the second flow controller 14 and the second electromagnetic valve 15 are all connected to a power supply device, which may be a battery pack or an external power supply.

[0066] The first flow controller 11 and the first connecting pipe 13 , the first solenoid valve 12 and the first connecting pipe 13 , the second flow controller 14 and the second connecting pipe 16 , and the second solenoid valve 15 and the second connecting pipe 16 are all sealedly connected.

[0067] The first connecting tube 13 and the second connecting tube 16 are made of medical polymer material.

[0068] The sampling device can be connected to various types of negative pressure suction devices, preferably a central negative pressure suction system, which is easy to use and can provide stable suction.

[0069] The sampling device can be connected to various types of sample liquid suction devices, and in addition to the sample liquid suction function, it can also include a flushing function with various water discharge modes.

[0070] The maximum capacity of the liquid storage container 2 is 500 ml, and the preset capacity of the liquid storage container 2 is generally 350 ml.

[0071] The preset capacity of the sampling tube is generally 50ml, and 5 tubes are usually required.

[0072] The process of using the sampling device for detecting tumor cell pleural and peritoneal metastasis is as follows:

[0073] 1) Preparation: The negative pressure suction device is connected to the negative pressure suction port 8 of the liquid storage container. One end of the sample liquid suction device is connected to the sampling assembly 1, and the other end is used to fully irrigate the patient's chest and abdominal cavity.

[0074] 2) Sampling process: Turn on the sampling control switch 20. When the negative pressure suction device extracts the air in the liquid storage container 2 through the vacuum pump, the closed liquid storage container 2 will generate negative pressure, prompting the sample liquid to flow from the sample liquid suction device along the drainage tube through the sampling component 1, and finally injected into the liquid storage container 2 from the liquid storage container inlet 7. When the capacity of the liquid storage container 2 reaches the preset capacity, the first flow controller 11 controls the first solenoid valve 12 to automatically close, and no more sample liquid is injected into the liquid storage container 2; at the same time, the negative pressure suction device and the sample liquid suction device are turned off. At this time, the sampling control switch 20 will automatically close.

[0075] 3) Stirring process: After stagnation for 30 to 60 seconds, turn on the stirring control switch 18. Since the magnetic core 6 can be subjected to a relatively uniform magnetic field force at each position in the liquid storage container 2, it performs regular circular motion, ensuring that the liquid can be uniformly stirred in the entire container. Generally, after stirring for 15 to 30 seconds, turn off the electric stirring control switch 18.

[0076] 4) Sampling process: At the same time, the sampling control switch 19 is turned on. The sample liquid flows from the liquid storage container outlet 9 at the bottom of the liquid storage container 2 through the sampling assembly 4, and finally injected into the sampling test tube from the sampling liquid outlet 17. When the sampling test tube reaches the preset capacity, the second flow controller 14 controls the second solenoid valve 15 to automatically close, and no more sample liquid is injected into the sampling test tube. At this time, the sampling control switch 19 is automatically closed.

[0077] 5) Repeat the sampling operation several times to obtain multiple tubes of sample liquid. In addition, since a small amount of sample liquid will remain in the connecting tube and cannot be fully stirred, in order to ensure the uniformity and consistency of the sample, the sample selection for inspection will start from the second tube.

[0078] The collection device of this embodiment will fully and evenly stir the lavage fluid sucked from the peritoneal cavity and accurately dispense it into multiple sampling tubes, avoiding the influence of factors such as sample liquid precipitation and human operation on the detection accuracy, ensuring the uniformity and consistency of the sample, and improving the accuracy of the detection of peritoneal metastasis of tumor cells. At the same time, the access to the sample liquid is automatically controlled by the control component (flow controller and solenoid valve), which is simple to operate and improves the sample processing efficiency.

[0079] The above description is only an embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent replacement and improvement made within the application scope of the present invention shall be included in the protection scope of the present invention.

Claims

1. A sampling device for detecting pleural and peritoneal metastasis of tumor cells, characterized in that: It includes a liquid storage container, a sampling component, a sampling component, an electromagnetic stirring component, a negative pressure suction device, and a sample liquid suction device; The liquid storage container is used to store sample liquid, and is provided with a liquid storage container liquid inlet and a liquid storage container negative pressure suction port at the top, and a liquid storage container liquid outlet at the bottom, and the negative pressure suction device is connected to the negative pressure suction port of the liquid storage container; The sampling assembly comprises a sampling liquid inlet and a first connecting tube, one end of the first connecting tube is connected to one end of the sampling liquid inlet, and the other end is connected to the liquid inlet of the liquid storage container, and the sample liquid suction device is connected to the other end of the sampling liquid inlet; The sampling assembly comprises a sampling liquid outlet and a second connecting tube, one end of the second connecting tube is connected to the liquid outlet of the liquid storage container, and the other end is connected to the sampling liquid outlet; The electromagnetic stirring assembly comprises: an electromagnetic stirrer arranged on the outer wall of the bottom of the liquid storage container, and a magnetic core arranged on the inner wall of the bottom of the liquid storage container; The sampling component and the sampling component are respectively provided with a sampling control switch and a sampling control switch.

2. A sampling device for detecting tumor cell pleural and peritoneal metastasis according to claim 1, characterized in that: A first flow controller and a first solenoid valve are sequentially arranged on the first connecting pipe of the sampling assembly according to the liquid flow direction; The first flow controller is provided with a control system, the input end of the control system is connected to the signal output end of the first flow controller, and the output end of the control system is connected to the first solenoid valve.

3. A sampling device for detecting tumor cell pleural and peritoneal metastasis according to claim 2, characterized in that: The first flow controller and the first solenoid valve are both connected to a power supply device.

4. A sampling device for detecting tumor cell pleural and peritoneal metastasis according to claim 2, characterized in that: The first flow controller and the first connecting pipe, as well as the first solenoid valve and the first connecting pipe are all sealed.

5. The sampling device for detecting tumor cell pleural and peritoneal metastasis according to claim 1, characterized in that: A second flow controller and a second solenoid valve are sequentially arranged on the second connecting pipe of the sampling assembly according to the liquid flow direction; The second flow controller is provided with a control system, the input end of the control system is connected to the signal output end of the second flow controller, and the output end of the control system is connected to the second solenoid valve.

6. A sampling device for detecting tumor cell pleural and peritoneal metastasis according to claim 5, characterized in that: The second flow controller and the second solenoid valve are both connected to a power supply device.

7. A sampling device for detecting tumor cell pleural and peritoneal metastasis according to claim 5, characterized in that: The second flow controller and the second connecting pipe, as well as the second solenoid valve and the second connecting pipe are all sealed.

8. A sampling device for detecting pleural and peritoneal metastasis of tumor cells according to claim 1, characterized in that: The electromagnetic stirring assembly is also provided with a stirring control switch, and the electromagnetic stirrer is connected to a power supply device.

9. A sampling device for detecting tumor cell pleural and peritoneal metastasis according to claim 1, characterized in that: The liquid storage container is made of any one of glass and medical polymer materials.

10. A sampling device for detecting tumor cell pleural and peritoneal metastasis according to claim 1, characterized in that: The first connecting tube and the second connecting tube are made of medical polymer material.

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