Brucella pathogenic microorganism extraction and detection integrated device

Abstract

The invention discloses a brucella pathogenic microorganism extraction and detection integrated device which comprises a rack, a first power shaft, a second power shaft and a third power shaft are rotatably connected to the inner bottom of the rack, and a vertical rod which is slidably connected with the rack and can be reset is arranged at the upper end of the rack in a penetrating mode. A centrifugal cylinder for extracting pathogenic microorganisms is fixed at the upper end of the second power shaft, and a detachable upper cover is mounted on the centrifugal cylinder. Automatic centrifugal layering of a pathogenic microorganism solution is achieved, serum is effectively extracted, the serum and normal saline can be mixed for dilution and directly discharged into the plastic funnel, the operation process is simplified, meanwhile, primary detection of pathogenic microorganisms is rapidly and accurately completed, and the detection efficiency is improved. The designed vibration mechanism ensures that residues in the hose are completely discharged, pollution is prevented, the reusability of equipment and the detection continuity are improved, and the detection accuracy is enhanced.

Description

Technical Field

[0001] The present invention relates to the technical field of pathogenic microorganism extraction and detection, and in particular to an integrated device for Brucella pathogenic microorganism extraction and detection. Background Art

[0002] Pathogenic microorganisms refer to microorganisms that can invade animals and cause infection or even infectious diseases, or pathogens; when animals are infected with diseases, it is necessary to study the pathogens that infect the animals, such as the pathogen detection device with publication number CN207779906U.

[0003] Currently, the extraction of pathogenic microorganisms is mostly done through centrifugation, followed by manual sampling. Specifically, after the centrifugation of the pathogenic microorganism solution is completed, the centrifuge is manually opened to take out the pathogenic microorganism extract sample from the centrifuge tube. This increases the labor intensity of the operators and also poses a risk of cross-contamination.

[0004] For example, Brucella is a type of Gram-negative, short rod-shaped bacillus that is most susceptible to infection in cattle, sheep, pigs and other animals, causing infectious abortion in female animals. Humans can be infected by contact with infected animals or by eating sick animals and their dairy products. Among them, the specific steps for extracting pathogenic microorganisms are: extracting animal serum through a syringe, and then centrifuging it through a centrifuge. The upper supernatant after centrifugation is the pathogenic microorganism extract; the extracted pathogenic microorganism extract can be detected by Brucella antibody rapid detection reagent (colloidal gold method), and the specific steps are: take a certain amount of human or animal serum specimen to be tested, dilute it with physiological saline, take a reagent, tear open the outer packaging, absorb the above-mentioned detection liquid, and add it dropwise into the reagent circular hole. Positive result: 2 red precipitation lines appear at "C" (control) and "T" (test) in the reagent display window, indicating that the Brucella antibody test is positive; negative result: only 1 red precipitation line appears at "C" in the reagent display window, indicating that the Brucella antibody test is negative; reagent failure: no red precipitation line appears at "C" in the reagent display window, regardless of whether a precipitation line appears at "T", it is judged as reagent failure.

[0005] In the above, the serum needs to be extracted separately, then diluted, and then added to the reagent circular wells. The above steps need to be completed step by step by the staff, which is time-consuming and laborious. In order to increase the accuracy of the test, multiple reagents need to be used for testing. This step also requires manual operation, and for multiple test tubes, the efficiency is low.

[0006] To this end, the present application proposes an integrated device for extracting and detecting Brucella pathogenic microorganisms. Summary of the invention

[0007] The purpose of the present invention is to solve the above technical problems and to propose an integrated device for extracting and detecting Brucella pathogenic microorganisms.

[0008] In order to achieve the above object, the present invention adopts the following technical solutions: The invention relates to an integrated device for extracting and detecting Brucella pathogenic microorganisms, comprising a frame, wherein the inner bottom of the frame is rotatably connected to a first power shaft, a second power shaft and a third power shaft, the first power shaft, the second power shaft and the third power shaft are driven to rotate by a driving mechanism, a sleeve is sleeved on the first power shaft and is rotatably connected to the first power shaft, a detection disk is fixedly connected to the upper end of the sleeve, a plurality of detection slots are provided on the detection disk, the first power shaft passes through the detection disk and the frame and is rotatably connected to the detection disk, a vertical rod is passed through the upper end of the frame and is slidably connected to the detection disk and can be reset, a centrifuge cylinder for extracting pathogenic microorganisms is fixed to the upper end of the second power shaft, a detachable upper cover is installed on the centrifuge cylinder, two L-shaped plates are installed on the inner top of the frame, a conveying cylinder is installed on the two L-shaped plates, a sealing piston connected to the vertical rod is slidably connected in the conveying cylinder, a medicine box is installed on one of the L-shaped plates, and the two conveying cylinders are respectively connected to the medicine box and the centrifuge cylinder.

[0009] Preferably, the driving mechanism includes a motor fixed on a frame, the output end of the motor is fixedly connected to a driving shaft, a first one-way bearing and a second one-way bearing are fixed on the driving shaft, a first transmission wheel is fixed on the first one-way bearing and the second power shaft, the two first transmission wheels are connected by a first transmission belt, a second transmission wheel is installed on the second one-way bearing and the third power shaft, the two second transmission wheels are connected by a second transmission belt, a first gear and a second gear are fixed on the sleeve and the first power shaft respectively, a first toothless gear and a second toothless gear are fixed on the third power shaft, the first toothless gear and the second toothless gear are meshed with the first gear and the second gear respectively.

[0010] Preferably, a connecting plate is fixed on the vertical rod, a first spring is fixed to the upper end of the connecting plate, the upper end of the first spring is fixedly connected to the frame, and the first spring is sleeved on the outside of the vertical rod.

[0011] Preferably, a second spring is fixed to the lower end of the sealing piston, the lower end of the second spring is fixedly connected to the conveying cylinder, and a pull rod is fixedly connected to the upper end of the sealing piston, and the pull rod is fixedly connected to the connecting disk.

[0012] Preferably, it also includes a connecting mechanism, which includes two short tubes respectively installed on the bottom of the two conveying cylinders, each of the two short tubes is equipped with a first one-way valve, each of the lower ends of the two short tubes is equipped with a hose, each of the upper ends of the two hoses is hingedly connected to a connecting rod, the connecting rod is hingedly connected to the vertical rod, and the bottom of the two conveying cylinders are respectively equipped with a first liquid inlet pipe and a second liquid inlet pipe, the first liquid inlet pipe is connected to the centrifuge cylinder, the second liquid inlet pipe is connected to the medicine box, and the first liquid inlet pipe and the second liquid inlet pipe are both equipped with a second one-way valve.

[0013] Preferably, a rotating joint is fixed to the other end of the first liquid inlet pipe, a metal tube is fixed to the bottom of the rotating joint, the metal tube passes through the upper cover and is fixedly connected to the upper cover, a rubber tube is fixed to the lower end of the metal tube, and a porous metal ball connected to the rubber tube is fixed to the bottom of the rubber tube.

[0014] Preferably, the first one-way valve only allows the fluid in the delivery cylinder to flow into the short tube.

[0015] Preferably, the second one-way valve only allows the fluid to flow into the delivery cylinder through the first liquid inlet pipe and the second liquid inlet pipe.

[0016] Preferably, a connecting plate is fixed to the upper end of the first power shaft, an arc-shaped plate is fixed on the connecting plate, both ends of the arc-shaped plate are provided with inclined surfaces, and a plurality of first convex strips are fixed to the bottom of the arc-shaped plate, a second convex strip is fixed to the upper end of the vertical rod, and the first convex strips and the second convex strips are located on the same circumference.

[0017] Compared with the prior art, the present invention has the following beneficial effects: 1. Under the drive of the first transmission wheel, the second power shaft rotates, and the rotation of the second power shaft drives the centrifuge cylinder and the upper cover to rotate, so that the pathogenic microorganism solution can be centrifuged, and finally the liquid is layered, and the serum is in the upper layer, thereby realizing the extraction of pathogenic microorganism serum.

[0018] 2. When the centrifuge cylinder and the upper cover rotate, due to the centrifugal force, the middle of the pathogenic microorganism solution is concave, and the rotation of the upper cover drives the metal tube, rubber tube and porous metal ball to rotate. Due to the centrifugal force, the porous metal ball drives the rubber tube to move and is almost in a horizontal position, so that the rubber tube and the porous metal ball can be separated from the pathogenic microorganism solution, and the rubber tube and the porous metal ball can be thrown out, so as to avoid the pathogenic microorganism solution remaining in the rubber tube and the porous metal ball from being unable to be effectively centrifuged, affecting subsequent detection.

[0019] 3. After centrifugation is completed, the motor stops working, and the rubber tube and the porous metal ball are reset under the action of gravity. At this time, the porous metal ball is located in the serum liquid for subsequent serum extraction.

[0020] 4. The motor reverses to drive the second one-way bearing to drive the second transmission wheel to rotate. When the first toothless gear is not engaged with the first gear, one of the detection slots on the detection disk is opposite to the hose, that is, the detection slot is located directly below the detection slot, so as to transport serum and saline.

[0021] 5. The vertical rod moves downward, driving the connecting plate downward, which squeezes the two pull rods downward, and the pull rods move downward to squeeze the sealing piston downward. The sealing piston moves downward to squeeze the serum and saline in the two conveying cylinders into the short tube, and finally discharge them through the hose; the vertical rod moves downward, driving the connecting rod downward, so that the two hoses are tilted downward to discharge the serum and saline.

[0022] 6. The serum and saline discharged through the hose collide with each other and fall into the plastic funnel. Since the serum and saline flow in the plastic funnel, the serum and saline are diluted and mixed.

[0023] 7. The first convex strip abuts against the second convex strip and moves relative to each other, so that the vertical rod shakes in a small range, that is, vibrates. The vibration of the vertical rod is transmitted to the connecting rod and the hose, and finally the hose vibrates, so that the residual serum and saline in the hose can be completely discharged to avoid dripping onto the test plate and contaminating the test plate.

[0024] In summary, the present invention realizes automatic centrifugal stratification of pathogenic microorganism solutions, effectively extracts serum, can mix serum with physiological saline for dilution, and directly discharges it into a plastic funnel, simplifies the operation process, and quickly and accurately completes the preliminary detection of pathogenic microorganisms. The designed vibration mechanism ensures the complete discharge of residues in the hose, prevents contamination, improves the reusability of the equipment and the continuity of detection, and enhances the accuracy of detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic structural diagram of an integrated device for extracting and detecting Brucella pathogenic microorganisms proposed by the present invention.

[0026] Figure 2 This is a schematic diagram of a Brucella pathogenic microorganism extraction and detection integrated device proposed by the present invention after a drive box is removed; Figure 3 This is a schematic diagram of the structure of a driving mechanism in an integrated device for extracting and detecting Brucella pathogenic microorganisms proposed by the present invention; Figure 4 This is a schematic diagram of the structure of the motor in the integrated device for extracting and detecting Brucella pathogenic microorganisms proposed by the present invention; Figure 5 This is a schematic diagram of the structure of the conveying cylinder in the integrated device for extracting and detecting Brucella pathogenic microorganisms proposed by the present invention; Figure 6 This is a cross-sectional view of a conveying cylinder in an integrated device for extracting and detecting Brucella pathogenic microorganisms proposed by the present invention; Figure 7 This is a disassembled diagram of a centrifuge and an upper cover in an integrated device for extracting and detecting Brucella pathogenic microorganisms proposed by the present invention; Figure 8 This is a schematic structural diagram of the arc plate in an integrated device for extraction and detection of Brucella pathogenic microorganisms proposed by the present invention.

[0027] In the figure: 1 frame, 2 detection plate, 3 detection slot, 4 cross frame, 5 sleeve, 6 first power shaft, 7 second power shaft, 8 centrifugal cylinder, 9 cover, 10 first liquid inlet pipe, 11 vertical rod, 12 second convex strip, 13 connecting plate, 14 arc plate, 15 connecting plate, 16 motor, 17 third power shaft, 18 medicine box, 19 drive shaft, 20 first transmission wheel, 21 first gear, 22 second gear, 23 first toothless gear, 24 second toothless gear, 25 first Transmission belt, 26 second transmission belt, 27 second transmission wheel, 28 first one-way bearing, 29 second one-way bearing, 30 first spring, 31 L-type plate, 32 conveying cylinder, 33 pull rod, 34 hose, 35 connecting rod, 36 first one-way valve, 37 second liquid inlet pipe, 38 second one-way valve, 39 rubber tube, 40 porous metal ball, 41 sealing piston, 42 second spring, 43 inclined surface, 44 first convex strip, 45 metal tube, 46 rotary joint, 47 short tube. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0029] Reference Figure 1-Figure 8 , an integrated device for extracting and detecting Brucella pathogenic microorganisms, comprising a frame 1, the frame 1 being in the shape of a rectangular frame; the inner bottom of the frame 1 is rotatably connected with a first power shaft 6, a second power shaft 7 and a third power shaft 17, the first power shaft 6, the second power shaft 7 and the third power shaft 17 are driven to rotate by a driving mechanism, the driving mechanism comprising a motor 16 fixed to the frame 1, the output end of the motor 16 being fixedly connected with a driving shaft 19, wherein a driving box is installed on the frame 1, the motor 16, the third power shaft 17, etc. are located in the driving box, such as Figure 1 shown.

[0030] A first one-way bearing 28 and a second one-way bearing 29 are fixed on the driving shaft 19, a first transmission wheel 20 is fixed on the first one-way bearing 28 and the second power shaft 7, the two first transmission wheels 20 are connected by a first transmission belt 25, a second transmission wheel 27 is installed on the second one-way bearing 29 and the third power shaft 17, the two second transmission wheels 27 are connected by a second transmission belt 26, a first gear 21 and a second gear 22 are fixed on the sleeve 5 and the first power shaft 6, respectively, a first toothless gear 23 and a second toothless gear 24 are fixed on the third power shaft 17, the first toothless gear 23 and the second toothless gear 24 are meshed with the first gear 21 and the second gear 22, respectively.

[0031] The first power shaft 6 is sleeved with a sleeve 5 rotatably connected thereto, a cross frame 4 is fixed on the frame 1, the sleeve 5 passes through the cross frame 4 and is fixedly connected thereto; the upper end of the sleeve 5 is fixedly connected to the detection disk 2, and the detection disk 2 is provided with a plurality of detection slots 3, the first power shaft 6 passes through the detection disk 2 and the frame 1 and is rotatably connected thereto, the upper end of the frame 1 is penetrated by a vertical rod 11 slidably connected thereto and capable of being reset, a connecting disk 15 is fixed on the vertical rod 11, a first spring 30 is fixed to the upper end of the connecting disk 15, the upper end of the first spring 30 is fixedly connected to the frame 1, and the first spring 30 is sleeved on the outside of the vertical rod 11.

[0032] A centrifuge cylinder 8 for extracting pathogenic microorganisms is fixed to the upper end of the second power shaft 7, and a detachable upper cover 9 is installed on the centrifuge cylinder 8. Two L-shaped plates 31 are installed on the inner top of the frame 1, and a conveying cylinder 32 is installed on the two L-shaped plates 31. A sealing piston 41 connected to the vertical rod 11 is slidably connected in the conveying cylinder 32, and a second spring 42 is fixed to the lower end of the sealing piston 41, and the lower end of the second spring 42 is fixedly connected to the conveying cylinder 32. A pull rod 33 is fixedly connected to the upper end of the sealing piston 41, and the pull rod 33 is fixedly connected to the connecting plate 15.

[0033] A medicine box 18 is installed on one of the L-shaped plates 31, and two conveying cylinders 32 are respectively connected to the medicine box 18 and the centrifugal cylinder 8, and also include a connecting mechanism, which includes two short tubes 47 respectively installed on the bottom of the two conveying cylinders 32. The two short tubes 47 are both installed with a first one-way valve 36, and the first one-way valve 36 only allows the fluid in the conveying cylinder 32 to flow into the short tube 47.

[0034] A hose 34 is installed at the lower end of the two short tubes 47, and a connecting rod 35 is hingedly connected to the upper end of the two hoses 34. The connecting rod 35 is hingedly connected to the vertical rod 11. The bottom of the two conveying cylinders 32 are respectively installed with a first liquid inlet pipe 10 and a second liquid inlet pipe 37. The first liquid inlet pipe 10 is connected to the centrifugal cylinder 8, and a rotating joint 46 is fixed to the other end of the first liquid inlet pipe 10. A metal tube 45 is fixed to the bottom of the rotating joint 46. The metal tube 45 passes through the upper cover 9 and is fixedly connected thereto. A rubber tube 39 is fixed to the lower end of the metal tube 45, and a porous metal ball 40 connected thereto is fixed to the bottom of the rubber tube 39.

[0035] The second liquid inlet pipe 37 is connected to the medicine box 18 , and the first liquid inlet pipe 10 and the second liquid inlet pipe 37 are both installed with a second one-way valve 38 , which only allows the fluid to flow through the first liquid inlet pipe 10 and the second liquid inlet pipe 37 into the delivery cylinder 32 .

[0036] In addition, a connecting plate 13 is fixed to the upper end of the first power shaft 6, and an arc-shaped plate 14 is fixed on the connecting plate 13. Both ends of the arc-shaped plate 14 are provided with inclined surfaces 43, and a plurality of first convex strips 44 are fixed to the bottom of the arc-shaped plate 14. A second convex strip 12 is fixed to the upper end of the vertical rod 11, and the first convex strips 44 and the second convex strips 12 are located on the same circumference.

[0037] When the present invention is used, multiple reagents are taken and the outer packaging is torn open (a plastic funnel can be installed in the reagent circular hole to collect the diluent, so as to facilitate entry into the reagent), and then placed in the detection tank 3, and then the pathogenic microorganism solution is added to the centrifugal cylinder 8, and then the upper cover 9 is covered, and the motor 16 is started. The motor 16 drives the drive shaft 19 to rotate. Under the action of the first one-way bearing 28 and the second one-way bearing 29, the second transmission wheel 27 and the drive shaft 19 rotate relative to each other. The rotation of the first one-way bearing 28 drives the first transmission wheel 20 to rotate. Under the drive of the first transmission belt 25, the second power shaft 7 rotates, and the rotation of the second power shaft 7 drives the centrifugal cylinder 8 and the upper cover 9 to rotate, so that the pathogenic microorganism solution can be centrifuged, and finally the liquid is layered, and the serum is in the upper layer, so that the pathogenic microorganism serum is extracted; When the centrifugal cylinder 8 and the upper cover 9 rotate, the middle of the pathogenic microorganism solution is concave due to the centrifugal force, and the upper cover 9 rotates to drive the metal tube 45, the rubber tube 39 and the porous metal ball 40 to rotate. Due to the centrifugal force, the porous metal ball 40 drives the rubber tube 39 to move and is almost in a horizontal position, so that the rubber tube 39 and the porous metal ball 40 can be separated from the pathogenic microorganism solution, and the rubber tube 39 and the porous metal ball 40 can be thrown out, so as to avoid the pathogenic microorganism solution remaining in the rubber tube 39 and the porous metal ball 40 from being effectively centrifuged, which affects the subsequent detection; After centrifugation is completed, the motor 16 stops working, and the rubber tube 39 and the porous metal ball 40 are reset under the action of gravity. At this time, the porous metal ball 40 is located in the serum liquid; Then, the motor 16 is started to reverse. Under the action of the first one-way bearing 28 and the second one-way bearing 29, the driving shaft 19 rotates to drive the second one-way bearing 29 and the second transmission wheel 27 to rotate. Under the transmission of the second transmission belt 26, the third power shaft 17 rotates. The third power shaft 17 rotates to drive the first toothless gear 23 and the second toothless gear 24. When the first toothless gear 23 is meshed with the first gear 21, the first toothless gear 23 is meshed with the first gear 21 to drive the sleeve 5 and the detection disk 2 to rotate. When the first toothless gear 23 is not meshed with the first gear 21, one of the detection slots 3 on the detection disk 2 is opposite to the hose 34, that is, the detection slot 3 is located directly below the hose 34. Figure 2 As shown, there are six detection slots 3, so the detection disk 2 rotates by 60° each time; As the third power shaft 17 rotates, the third power shaft 17 drives the second toothless gear 24 to rotate and mesh with the second gear 22. Each rotation angle is 180°. The rotation of the second gear 22 drives the first power shaft 6 to rotate, and then drives the connecting plate 13, the arc plate 14 and the first convex strip 44 to rotate. As the arc plate 14 rotates, the inclined surface 43 first abuts against the vertical rod 11, and can drive the vertical rod 11 to move downward under pressure. Finally, the second convex strip 12 slides against the bottom of the arc plate 14. The vertical rod 11 moves downward to drive the connecting plate 15 to move downward, and the connecting plate 15 moves downward to squeeze the two pull rods 33 to move downward, and the pull rods 33 move downward to squeeze the sealing piston 41 to move downward. The sealing piston 41 moves downward to squeeze the serum and saline in the two conveying cylinders 32 into the short tube 47, and finally discharge them through the hose 34; wherein, the vertical rod 11 moves downward to drive the connecting rod 35 to move downward, so that the two hoses 34 are tilted downward to discharge the serum and saline; The serum and saline discharged through the hose 34 collide with each other and fall into the plastic funnel and enter the reagent circular hole. Since the serum and saline flow in the plastic funnel, the serum and saline are mixed and diluted; Positive result: Two red precipitation lines appear in the reagent display window "C" (control) and "T" (test), indicating that the Brucella antibody test is positive.

[0038] Negative result: Only one red precipitation line appears at "C" in the reagent display window, indicating that the Brucella antibody test is negative.

[0039] Reagent failure: If no red precipitation line appears at "C" in the reagent display window, the reagent is judged to be failed regardless of whether a precipitation line appears at "T".

[0040] As the arc plate 14 rotates, the first convex strip 44 and the second convex strip 12 abut against each other and move relative to each other, so that the vertical rod 11 shakes in a small range, that is, vibrates. The vibration of the vertical rod 11 is transmitted to the connecting rod 35 and the hose 34, and finally the hose 34 vibrates, so that the residual serum and physiological saline in the hose 34 can be completely discharged to avoid dripping on the test disk 2 and causing contamination to the test disk 2. When the arc plate 14 is separated from the vertical rod 11, the connection plate 15 and the sealing piston 41 can be reset under the action of the first spring 30 and the second spring 42, and the serum can be sucked into the delivery cylinder 32 through the porous metal ball 40, the rubber tube 39, the metal tube 45 and the first liquid inlet tube 10, and the physiological saline in the medicine box 18 can be sucked into the delivery cylinder 32 through the second liquid inlet tube 37 for next use; When the second toothless gear 24 is not meshed with the second gear 22, the arc plate 14 is reset, and when the first toothless gear 23 is meshed with the first gear 21, the detection disk 2 is driven again to rotate 60° and remain stationary. By repeating the above steps, the serum can be tested again to understand whether the animal is sick. Through multiple tests, the accuracy of the test can be ensured.

[0041] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. An integrated device for extracting and detecting Brucella pathogenic microorganisms, comprising a frame (1), characterized in that: The inner bottom of the frame (1) is rotatably connected to a first power shaft (6), a second power shaft (7) and a third power shaft (17); the first power shaft (6), the second power shaft (7) and the third power shaft (17) are driven to rotate by a driving mechanism; a sleeve (5) is sleeved on the first power shaft (6) and is rotatably connected to the first power shaft; the upper end of the sleeve (5) is fixedly connected to a detection disk (2); the detection disk (2) is provided with a plurality of detection slots (3); the first power shaft (6) passes through the detection disk (2) and the frame (1) and is rotatably connected thereto; the upper end of the frame (1) passes through a sleeve (5) and is slidably connected thereto; The second power shaft (7) is provided with a centrifugal cylinder (8) for extracting pathogenic microorganisms fixed to its upper end, and a detachable upper cover (9) is installed on the centrifugal cylinder (8). Two L-shaped plates (31) are installed on the inner top of the frame (1), and a conveying cylinder (32) is installed on each of the two L-shaped plates (31). A sealing piston (41) connected to the vertical rod (11) is slidably connected in the conveying cylinder (32), and a medicine box (18) is installed on one of the L-shaped plates (31). The two conveying cylinders (32) are respectively connected to the medicine box (18) and the centrifugal cylinder (8).

2. The integrated device for extracting and detecting Brucella pathogenic microorganisms according to claim 1, characterized in that: The driving mechanism comprises a motor (16) fixed on a frame (1); an output end of the motor (16) is fixedly connected to a driving shaft (19); a first one-way bearing (28) and a second one-way bearing (29) are fixed on the driving shaft (19); a first transmission wheel (20) is fixed on the first one-way bearing (28) and the second power shaft (7); the two first transmission wheels (20) are connected via a first transmission belt (25); a second transmission wheel (27) is installed on the second one-way bearing (29) and the third power shaft (17); the two second transmission wheels (27) are connected via a second transmission belt (26); a first gear (21) and a second gear (22) are fixed on the sleeve (5) and the first power shaft (6), respectively; a first toothless gear (23) and a second toothless gear (24) are fixed on the third power shaft (17); the first toothless gear (23) and the second toothless gear (24) are meshed with the first gear (21) and the second gear (22), respectively.

3. The integrated device for extracting and detecting Brucella pathogenic microorganisms according to claim 1, characterized in that: A connecting plate (15) is fixed on the vertical rod (11), a first spring (30) is fixed on the upper end of the connecting plate (15), the upper end of the first spring (30) is fixedly connected to the frame (1), and the first spring (30) is sleeved on the outside of the vertical rod (11).

4. The integrated device for extracting and detecting Brucella pathogenic microorganisms according to claim 3, characterized in that: A second spring (42) is fixed to the lower end of the sealing piston (41), and the lower end of the second spring (42) is fixedly connected to the conveying cylinder (32). A pull rod (33) is fixedly connected to the upper end of the sealing piston (41), and the pull rod (33) is fixedly connected to the connecting plate (15).

5. The integrated device for extracting and detecting Brucella pathogenic microorganisms according to claim 1, characterized in that: The invention also comprises a connection mechanism, wherein the connection mechanism comprises two short tubes (47) respectively mounted on the bottom of the two conveying cylinders (32), the two short tubes (47) are each mounted with a first one-way valve (36), the lower ends of the two short tubes (47) are each mounted with a hose (34), the upper ends of the two hoses (34) are each hingedly connected with a connecting rod (35), the connecting rod (35) is hingedly connected to a vertical rod (11), the bottoms of the two conveying cylinders (32) are respectively mounted with a first liquid inlet pipe (10) and a second liquid inlet pipe (37), the first liquid inlet pipe (10) is connected to the centrifugal cylinder (8), the second liquid inlet pipe (37) is connected to the medicine box (18), and the first liquid inlet pipe (10) and the second liquid inlet pipe (37) are each mounted with a second one-way valve (38).

6. The integrated device for extracting and detecting Brucella pathogenic microorganisms according to claim 5, characterized in that: A rotating joint (46) is fixed to the other end of the first liquid inlet pipe (10), a metal tube (45) is fixed to the bottom of the rotating joint (46), the metal tube (45) passes through the upper cover (9) and is fixedly connected to the upper cover (9), a rubber tube (39) is fixed to the lower end of the metal tube (45), and a porous metal ball (40) connected to the rubber tube (39) is fixed to the bottom of the rubber tube (39).

7. The integrated device for extracting and detecting Brucella pathogenic microorganisms according to claim 5, characterized in that: The first one-way valve (36) only allows the fluid in the delivery cylinder (32) to flow into the short tube (47).

8. The integrated device for extracting and detecting Brucella pathogenic microorganisms according to claim 5, characterized in that: The second one-way valve (38) only allows the fluid to flow into the delivery cylinder (32) through the first liquid inlet pipe (10) and the second liquid inlet pipe (37).

9. The integrated device for extracting and detecting Brucella pathogenic microorganisms according to claim 1, characterized in that: A connecting plate (13) is fixed to the upper end of the first power shaft (6), an arc-shaped plate (14) is fixed to the connecting plate (13), both ends of the arc-shaped plate (14) are provided with inclined surfaces (43), and a plurality of first convex strips (44) are fixed to the bottom of the arc-shaped plate (14), a second convex strip (12) is fixed to the upper end of the vertical rod (11), and the first convex strip (44) and the second convex strip (12) are located on the same circumference.

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