Method for in-vitro induction of tulip tree cluster buds and regeneration seedlings

By using the apical buds or axillary buds cut from tender branches as explants on North American lemons and induced culture in specific culture media, an ex vivo regeneration system was established, which solved the problems of low reproduction rate and difficulty in rooting of lemons, and achieved efficient clump bud induction and rooting rate improvement.

CN119699190AActive Publication Date: 2025-03-28SHANDONG ACADEMY OF AGRICULTURAL SCIENCES +3
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Patent Information

Application Number
CN202510164240.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-14
Publication Date
2025-03-28
Estimated Expiration
2045-02-14

AI Technical Summary

Technical Problem

The reproduction rate of plants of the genus genus sac is low, the seed fruiting rate is low, the germination rate is low, and the cutting rooting rate is also low. The rapid reproduction of tissue culture has problems such as easy browning of explants, endophyte contamination, low reproduction coefficient, and difficulty in rooting, which affects the establishment and application of an efficient and rapid breeding system.

Method used

The apical bud or axillary bud was cut as explants by using North American lemon lemons. After disinfection, the induction culture medium, bud elongation culture medium and rooting culture medium were carried out to establish an ex vivo regeneration system.

Benefits of technology

Effectively remove endophytic contamination in plants, improve the induction and elongation of clustered buds, enhance the rooting ability, and the rooting rate can reach 73.3%, solving the problems of difficulty in reproduction and rooting in the existing technology, and providing technical support for the efficient reproduction of lemonacea plants.

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Abstract

The invention discloses a method for in-vitro induction of tulip tree cluster buds and regeneration seedlings. The method comprises the following steps: firstly, cutting 3-4cm of a plant material containing terminal buds or axillary buds from a liriodendron twig, disinfecting, removing plant endophyte, then cutting 0.5-1cm of the terminal buds or axillary buds as explants, then putting the explants on a cluster bud induction culture medium for induction culture for about 30 days, then cutting off the induced cluster buds, and putting the induced cluster buds on an elongation culture medium for culture; when the buds extend to be rootless seedlings with the length of 2-3 cm, the seedlings are transferred to a rooting culture medium for rooting induction, and the seedlings can root after being cultured in the rooting culture medium for about 40 days. According to the method, terminal buds and axillary buds are used as explants, growth of endophytes is effectively controlled, a cluster bud induction culture medium, a bud elongation culture medium and a rooting culture medium are screened out, an in-vitro regeneration system is established, and technical support is provided for propagation of liriodendron plants.
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