Anti-inflammatory and anti-allergic Li medicine and preparation method thereof

Abstract

The invention discloses an anti-inflammatory and anti-allergic Li medicine and a preparation method thereof, and the process is as follows: on the basis of an original Li medicine prescription, Yaja pine pollen is newly added, and the anti-inflammatory and anti-allergic Li medicine is developed. The anti-inflammatory, anti-allergic and itching-relieving experiments are carried out on the Li medicine, and the results fully prove that the Yaja pine pollen, the anti-inflammatory and anti-allergic traditional Li medicine and the Li medicine compounded by the Yaja pine pollen and the traditional Li medicine have the anti-allergic, anti-inflammatory and itching-relieving effects. In addition, the effect of the anti-inflammatory and anti-allergic Li medicine is better than that of the independently used Yaja pine pollen and the traditional Li medicine prescription, so that the development space and way are broadened for protecting and inheriting the proved recipe of the Li medicine.

Description

Technical Field

[0001] The invention relates to an anti-inflammatory and anti-allergic Li medicine and a preparation method thereof, and belongs to the field of ethnic medicine. Background Art

[0002] The prescription is composed of four Li medicinal materials: Lantana camara, Artemisia dahurica, Artemisia dafeng and Artemisia ulmoides.

[0003] Hainan Li medicine is an important part of the Chinese nation's treasure house and a great treasure of the country's traditional ethnic medicine. The Li culture has no written language, only language, and many Li medicine secrets can only rely on oral transmission from ancestors. With the development of the times, many prescriptions are facing the risk of being lost. Therefore, the excavation, inheritance and improvement of Li medicine prescriptions is undoubtedly a rescue excavation and protection of Li medicine.

[0004] The traditional Li medicine prescription for anti-inflammatory and anti-allergic uses the external application method according to the symptoms when administering. The above four fresh leaves or the whole plant are mashed and applied to the affected area, or fresh (dry) branches and leaves or roots are boiled in water and washed with water. This method is suitable for skin itching and eczema. For those with skin erosion, an appropriate amount of the four Li medicine dry powder can be sprinkled on the affected area for treatment. However, in the actual application process, it was found that the dosage and quality of the external application and lotion were difficult to control, it was inconvenient to carry and use, it was easy to mold, and it was not easy to preserve, which limited its use. In addition, it was proved by experiments that the original prescription also had shortcomings in efficacy. Therefore, improvements were made to the above shortcomings. On the basis of the original traditional Li medicine prescription for anti-inflammatory and anti-allergic, Yajia pine pollen was added, and two dosage forms were developed using modern pharmaceutical production technology. The first is a press-type cream, which can be applied to the affected area when used. The cream has good stability, sustained release and moisturizing properties, is clean and hygienic, and is easy to carry, which significantly improves the efficacy. The second is to make Yajia Songhua Powder, which is suitable for infant eczema, thus improving the shortcomings of the original Li medicine prescription.

[0005] Pinus massoniana var. hainanensis WC Cheng & LK Fu is a variant of Pinus massoniana, mainly distributed in Hainan Province. Pinus massoniana pollen has a long history of application in the Li ethnic group-inhabited areas of Hainan. It is mainly used for external use and is suitable for children's eczema, skin itching and ulcers. Pinus massoniana pollen has anti-inflammatory, analgesic, antibacterial and anti-allergic pharmacological activities. Its main functions are drying dampness and astringency, and it is used for traumatic bleeding and impetigo. Pinus massoniana pollen has anti-allergic, anti-inflammatory and antipruritic effects. Therefore, Pinus massoniana pollen is compounded with traditional Li medicine prescriptions that can resist inflammation and allergy, and an anti-inflammatory and anti-allergic Li medicine is developed. According to literature search, there is no report on the use of Pinus massoniana pollen in medicine.

[0006] Lantana is a plant of the genus Lantana in the Verbenaceae family, Lantana camara L., also known as five-color plum, Ruyi grass, seven-change flower, and stinky grass. Its roots are cool in nature and sweet in taste, its leaves are cold in nature and bitter and pungent in taste, its flowers are cool in nature and sweet and light in taste, and it enters the lung and liver meridians. It has the effects of clearing away heat and detoxifying, dispersing blood stasis and reducing swelling, and dispelling wind and relieving itching. Its chemical components include volatile oils, triterpenes, flavonoids, and phenylethanol glycosides.

[0007] Euphorbia biahirta, also known as Euphorbia biahirta, Euphorbia biahirta, is the dried whole herb of Euphorbia biahirta L., Euphorbiaceae. It tastes spicy, sour, cool in nature, and slightly toxic. It enters the lung, bladder, and large intestine meridians. It is distributed in Guangdong, Hainan, Yunnan, and other places in my country. It has the effects of promoting dampness and relieving itching, clearing away heat and detoxifying, and promoting lactation. It is mainly used to treat eczema, dermatitis, skin itching, lung abscess, and breast abscess. Euphorbia biahirta mainly contains flavonoids, coumarins, lignans, triterpenes, steroids, tannins, alkaloids, and other chemical components.

[0008] Artemisia balsamifera, also known as Blumea balsamifera, is the dried aerial part of the Asteraceae plant Blumea balsamifera. It has the effects of warming the middle and activating blood circulation, regulating menstruation, and removing wind and dampness. This medicinal material mainly contains volatile oil (monoterpenoid), flavonoids, and sesquiterpenoid components.

[0009] Embelia burm is the dried leaves of Embelia laeta (L.) Mez and E. ribes Burm, plants of the genus Embelia Burm. f. of the family Myrsinaceae. Embelia burm leaves can treat vitamin C deficiency, anemia, etc. They can also be mashed and applied to bruises and swellings, and the juice can be applied to itchy skin. Embelia burm is rich in quinones, flavonoids, steroids, phenolic acids, volatile oils and other ingredients. Summary of the invention

[0010] In view of this, the present invention provides an anti-inflammatory and anti-allergic Li medicine and a preparation method, which facilitates the subsequent excavation and exploration of traditional Li medicine prescriptions, and also broadens the development space and path for the protection and inheritance of Li medicine prescriptions.

[0011] In order to achieve the above purpose, the following technical solutions are adopted:

[0012] An anti-inflammatory and anti-allergic Li medicine and a preparation method thereof, comprising the following steps:

[0013] Preparation of the Herba Lysimachiae Extract

[0014] Lantana extract: Take 100g of dried Lantana root or leaf and crush it with a grinder. Place the crushed medicinal material in a reflux extraction tank and add 50% (V / V) ethanol for reflux extraction at 70°C for a total of 3 times, each time for 1 hour. Add 500 ml of ethanol for the first time, 300 ml for the second time, and 200 ml for the third time. After combining the three extracts, distill and recover the ethanol under reduced pressure, concentrate the extract, and finally freeze-dry the extract to obtain the extract.

[0015] Extract of Da Fei Yang: The whole Da Fei Yang herb is collected and dried in the shade, crushed by a grinder, and the obtained plant powder is stored in a sealed bag in a light-shielded place for later use. Weigh 100g of the sample, place it in a reflux extraction tank, add 50% (V / V) ethanol and reflux extract 3 times at 70℃, reflux for 1h each time. Add 500ml of the above ethanol for the first time, 300ml for the second time, and 200ml for the third time. Combine the three extracts, distill and recover ethanol under reduced pressure, concentrate the extract, and finally freeze-dry the extract to obtain the extract.

[0016] Artemisia argyi extract: Weigh the dried whole herb of Artemisia argyi and crush it. Place 100g of the obtained powder in a reflux extraction tank. Add 50% (V / V) ethanol and reflux for extraction 3 times at 70°C, reflux for 1h each time. Add 500ml 3L of the above ethanol for the first time, 300ml for the second time, and 200ml for the third time. Combine the three extracts, distill and recover the ethanol under reduced pressure, concentrate the extract, and finally freeze-dry the extract to obtain the extract.

[0017] Extract of Atractylodes lancea: Take 100g of dried Atractylodes lancea leaves, crush them, place them in a reflux extraction tank, add 50% (V / V) ethanol and reflux for 3 times at 70°C, reflux for 1h each time. Add 500ml of the above ethanol for the first time, 300ml for the second time, and 200ml for the third time. Combine the three extracts, distill and recover ethanol under reduced pressure, concentrate the extract, and finally freeze-dry the extract to obtain the extract.

[0018] Preparation of pine pollen

[0019] Pine pollen: Unbroken pine pollen was collected from Hainan Bawangling National Nature Reserve and was identified as pine pollen by Researcher Wang Yong from the School of Pharmacy of Hainan Medical University.

[0020] Method: Weigh 3g of unbroken wall Pinus japonici pollen sample and put it into a 50mL grinding tube (so that the sample volume accounts for about 1 / 3 of the total volume of the grinding tube), then put in the grinding glass ball, cover the tube tightly, and after balancing, put the grinding tube and the grinding tool into a -20℃ refrigerator. After the sample and the grinding tool are cooled to -20℃, put the sample and the grinding tool into a grinder for grinding. Under the conditions of grinding frequency of 58Hz, grinding time of 290s and grinding ball diameter of 4mm, the wall breaking rate of Pinus japonici pollen can reach (93.88±0.78)%, and finally Pinus japonici pollen is obtained.

[0021] In order to explore the composition of pine pollen, Beijing Zhongke Baice Testing Technology Co., Ltd. was commissioned to conduct testing:

[0022] 1. Detection of volatile components of Yakapine pollen: Gas chromatography-mass spectrometry (GCMS), Agilent, 7890B-5977B Weigh 0.382g of all samples into a headspace bottle. Solid phase microextraction (solid phase microextraction fiber: DVB / CAR / PDMS (LabTech)) was performed at 45°C, with a shaking time of 15min, an adsorption time of 30min, and a desorption test at 250°C for 5min. Column: DB-5ms+DG 30 mx 0.25 mm x 0.2.

[0023] The volatile components of pine pollen were tested by Beijing Zhongke Baice Testing Technology Co., Ltd. (report number: ZKBC240524-394):.

[0025]

[0026]

[0027] The conclusion is: it contains ingredients that clear heat, relieve pain and inhibit bacteria.

[0028] 2. Test results of non-volatile components of P. jasminoides pollen.

[0029] 1. Dry Sample Extraction

[0030] (1) The biological samples were placed in a freeze dryer (Scientz-100F) and vacuum-frozen for 63 h;

[0031] (2) Grinding (30 Hz, 1.5 min) into powder using a grinder (MM 400, Retsch);

[0032] (3) Weigh 50 mg of sample powder using an electronic balance (MS105DM), add 1200 μL of -20°C precooled 70% methanol water internal standard extract (if less than 50 mg, add 1200 μL of extractant for every 50 mg of sample). The internal standard extract is prepared by dissolving 1 mg of standard in 1 mL of 70% methanol water to prepare a 1000 μg / mL standard stock solution, and the 1000 μg / mL stock solution is further diluted with 70% methanol to prepare a 250 μg / mL internal standard solution;

[0033] (4) Vortex once every 30 minutes for 30 seconds each time, for a total of 6 times;

[0034] (5) After centrifugation (12,000 rpm, 3 min), aspirate the supernatant, filter the sample using a microporous filter membrane (0.22 μm pore size), and store it in a sample injection bottle for UPLC-MS / MS analysis.

[0035] 2. Chromatographic conditions

[0036] (1) Chromatographic column: Waters ACQUITY Premier HSS T3 Column 1.8 µm, 2.1 mm * 100 mm

[0037] (2) Mobile phase A: 0.1% formic acid / water; Mobile phase B: 0.1% formic acid / acetonitrile

[0038] (3) Instrument column temperature: 40 ° C; flow rate: 0.4 mL / min; injection volume: 4 μ.

[0039]

[0040] 3. Mass spectrometry conditions

[0041]

[0043] .

[0044]

[0045]

[0046]

[0047]

[0048]

[0049]

[0050] The conclusion is: there are many anti-inflammatory, antibacterial and anti-allergic ingredients.

[0051] Preparation of an anti-inflammatory and anti-allergic medicine

[0052] Implementation Plan 1

[0053] Jacaranda pollen 60%, Lantana camara extract 15%, Artemisia selengensis extract 10%, Artemisia argyi extract 10%, Atractylodes lancea extract 5%,

[0054] The total weight reaches 100%, and the above drugs are ground into powder, fully mixed and made into tablets, each tablet is 1g, and 2 tablets are taken orally before each meal, 3 times a day.

[0055] Implementation Plan 2

[0056] 60% jasmine pollen, 15% lantana extract, 10% cyperus rotundus extract, 10% wormwood extract, 5% sour vine extract

[0057] The total weight reaches 100g, and the above medicines are ground into powder, soaked in rice wine and filtered to make a spray, 20g per bottle, use an appropriate amount, spray on the patient's lesions several times a day.

[0058] Implementation Plan 3

[0059] 60g of pine pollen, 15g of Lantana camara extract, 10g of Artemisia selengensis extract, 10g of Artemisia selengensis extract, 5% of Atractylodes lancea extract

[0060] The total weight reaches 100g, and the above drugs are ground into powder, mixed thoroughly and evenly to make a powder. Apply an appropriate amount of the powder to the affected area 3 times a day.

[0061] Implementation Plan 4

[0062] 60g of pine pollen, 15g of Lantana camara extract, 10g of Artemisia selengensis extract, 10g of Artemisia selengensis extract, 5g of Atractylodes lancea extract

[0063] The total weight reaches 100g, and the above drugs are ground and mixed evenly to make powder, and then 100g of ordinary honey is added to fully blend, and evenly divided into 50 portions to make honey pills, each weighing 4g. The patient takes 1 pill orally before each meal, 3 times a day.

[0064] Implementation Plan 5

[0065] 60g of pine pollen, 15g of Lantana camara extract, 10g of Artemisia selengensis extract, 10g of Artemisia selengensis extract, 5g of Atractylodes lancea extract

[0066] The total weight reaches 100g, and the above-mentioned drugs are ground into powder, mixed thoroughly and evenly, made into powder, and loaded into medicinal glutinous rice capsules. The net weight of each capsule is 0.5g. The patient takes 4 capsules orally before each meal, 3 times a day.

[0067] Implementation Plan 6

[0068] 60g of pine pollen, 15g of Lantana camara extract, 10g of Artemisia selengensis extract, 10g of Artemisia selengensis extract, 5g of Atractylodes lancea extract

[0069] The total weight reaches 100g, and the above medicines are ground into powder, mixed thoroughly and evenly, made into granules, packed in small medicinal paper bags and sealed. The net weight of each bag is 2g. The patient takes 1 bag before each meal, 3 times a day.

[0070] Implementation Plan 7

[0071] According to the following weight ratio: 60g of pine pollen, 15g of Lantana camara extract, 10g of Artemisia selengensis extract, 10g of Artemisia selengensis extract, 5g of Atractylodes lancea extract

[0072] The above-mentioned drugs are ground into fine powder, mixed thoroughly and evenly, and made into tinctures, creams, gels, ointments, jasmine powder, plasters and other external preparations.

[0073] It can be seen from the above technical scheme that compared with the prior art, the present invention discloses an anti-inflammatory and anti-allergic Li medicine and a preparation method. On the basis of the traditional anti-inflammatory and anti-allergic Li medicine prescription, yagapine pollen was added to develop a Li medicine. After anti-allergic, anti-inflammatory and antipruritic experiments on mice, the results showed that the anti-inflammatory and anti-allergic effects of the traditional Li medicine prescription and yagapine pollen were better than those of the traditional Li medicine prescription and yagapine pollen. This provides scientific data for the protection and inheritance of Li medicine prescriptions, secondary innovation and the construction of Li medicine brands. BRIEF DESCRIPTION OF THE DRAWINGS

[0074] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0075] Figure 1 Comparison of mouse ear swelling for anti-allergic test results

[0076] Figure 2 Anti-inflammatory test results DETAILED DESCRIPTION

[0077] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the implementation cases described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0078] Experimental instruments and equipment

[0079] Model: 800A Fang Taitai multifunctional pulverizer Dongguan Fang Tai Electric Co., Ltd., refrigerator (Qingdao Haier Co., Ltd.), BCD-26TS digital display constant temperature water bath (Jintan Shenglan Instrument Co., Ltd.), KW-1000DC electric constant temperature blast drying oven (Shanghai Jinghong Experimental Equipment Co., Ltd.), DHG-9030AXinyi-48 high-throughput tissue grinder (with freezing module) (Ningbo Xinyi Ultrasonic Equipment Co., Ltd.), WH-3 micro vortex mixer (Haihuxi Analytical Instrument Factory Co., Ltd.), E220G biological microscope (McOdie Industrial Group China Co., Ltd.), PGX-280A-12HM ultrapure water machine (Sichuan Youpu Ultrapure Technology Co., Ltd.), B0510C rotary evaporator (Chongqing Yamato Technology Co., Ltd.), Junde brand freeze dryer FD-503, heat reflux device, FA1204G balance (Changzhou Wantai Balance Instrument Co., Ltd.), KQ-B electronic temperature control universal furnace (Tianjin Test Instrument Co., Ltd.).

[0080] Experimental consumables and chemical reagents

[0081] Hydrocortisone butyrate cream was produced by Zhejiang Xianju Pharmaceutical Co., Ltd. with a batch number of ML2316. Compound dexamethasone acetate cream was produced by Hubei Ketian Pharmaceutical Co., Ltd. with a product batch number of 230210. 1-Chloro-2.4-dinitrochlorobenzene was purchased from Beijing Bailingwei Technology Co., Ltd. with a product number of 443475. 4-Aminopyridine (4-AP) and acetone were provided by Hainan Tropical Ocean University, and xylene was purchased from Guangdong Fine Chemical Engineering Technology Research and Development Center with a batch number of 2310242; Li Danlan Herbal Antibacterial Gel was produced by Li Danlan (Hainan) Biomedical Technology Co., Ltd. with a batch number of HM24-03Y02D1. Unbroken wall pine pollen was collected from Bawangling, Hainan Province. Pine pollen, traditional Li medicine for anti-inflammatory and anti-allergic effects, anti-inflammatory and anti-allergic Li medicine, sacha inchi oil, and distilled water were all made in the laboratory.

[0082] Experimental methods

[0083] (1) Drug preparation

[0084] Hydrocortisone butyrate cream: Accurately weigh 0.12 g and apply 0.12 g to each mouse.

[0085] Compound dexamethasone acetate cream: accurately weigh 0.12 g and apply 0.12 g to each mouse.

[0086] Lidanlan Herbal Antibacterial Gel: Accurately weigh 0.12g and apply 0.12g to each mouse.

[0087] Pine pollen: weigh 0.6g accurately and place it in a 100mL volumetric flask, add purified water to the mark, dissolve thoroughly and shake well. Apply 0.2mL (containing 1.2mg) to each mouse.

[0088] Traditional Li medicine for anti-inflammatory and anti-allergic effects: Accurately weigh 0.6 g and place in a 100 mL volumetric flask, add purified water to the mark, fully dissolve and shake well. Apply 0.2 mL (containing 1.2 mg) to each mouse.

[0089] Anti-inflammatory and anti-allergic medicine: Accurately weigh 0.6g and place it in a 100mL volumetric flask, add purified water to the mark, fully dissolve and shake well. Apply 0.2mL (containing 1.2mg) to each mouse.

[0090] (2) Experimental animals: ICR mice were purchased from Hunan Slake Jingda Experimental Animal Co., Ltd. (SPF grade), license number SYXk(Xiang)2019-0004; depilatory cream was purchased from Shenzhen Mengguang Cosmetics Co., Ltd., batch number: 2023082305; 8 mm hole puncher, funnel, flask, beaker, glass rod, gauze, filter paper, measuring cylinder, mouse cage.

[0091] Experimental methods

[0092] 1. Anti-allergic test:

[0093] 1. Modeling experiment: 60 healthy mice were randomly divided into 5 groups, including 12 model control groups, 12 positive control groups, 12 pine pollen experimental groups, 12 anti-inflammatory and anti-allergic traditional Li medicine control groups, and 12 anti-inflammatory and anti-allergic Li medicine experimental groups. The abdomen of the mice in each group was depilated by about 2.5 cm × 2.5 cm. After depilation, the abdomen of the mice was sensitized once with 50 μL 7% 1-chloro-2,4-dinitrobenzene-acetone-saturnea inchi oil solution (3:1, V / V), and sensitization was repeated the next day. On the 6th, 9th, 10th, 13th, and 16th days of the experiment, 20 μL 0.5% 1-chloro-2,4-dinitrobenzene-acetone-saturnea inchi oil solution was used to stimulate the inside and outside of the right ear of the mice multiple times. After 10 days, the appearance of redness, swelling, hypertrophy, and other pathological symptoms on the right ear of the mice was used as the standard for successful modeling.

[0094] 2. Experiments after successful modeling: The mice that failed to model were eliminated, and 50 mice with successful modeling were divided into 5 groups: A, 10 positive control groups, hydrocortisone butyrate cream was applied to the right ears of the mice; B, 10 model groups, distilled water was applied to the right ears of the mice; C, 10 yagapine pollen experimental groups, yagapine pollen was applied to the right ears of the mice; D, 10 traditional Li medicine control groups for anti-inflammatory and anti-allergic treatment, traditional Li medicine was applied to the right ears of the mice; C, 10 anti-inflammatory and anti-allergic Li medicine experimental groups, Li medicine was applied to the right ears of the mice. Another 10 mice were selected as the normal control group, and distilled water was applied to the right ears of the mice. The above groups were applied once in the morning and once in the afternoon every day for 7 consecutive days. 30 minutes after the last administration, except for the normal control group, the other groups were applied with 0.5% 1-chloro-2,4-dinitrobenzene-acetone-sacha inchi oil to stimulate allergic reactions in the right ears of the mice. After 20 hours, the mice were killed by dislocation, the auricles of both ears were cut off, and the round ear pieces were punched in the same part of the left and right ears with an 8 mm puncher. The weight was weighed using an electronic balance, and the thickness of the middle part of the left and right ears of the mice was measured to calculate the ear swelling. The experiment was repeated 3 times in parallel. The formula for calculating ear swelling (%) is: (right ear thickness-left ear thickness) × 100%.

[0095] Anti-allergic test results:

[0096] Table 1 Comparison of mouse ear swelling ( ,%, n=10)

[0097]

[0098] Compared with the normal control group, # P<0.05 or ## P<0.01; compared with the model group, * P<0.05 or ** P<0.01.

[0099] Conclusion: Compared with the model group, the positive control group (hydrocortisone butyrate cream), jasmine pollen experimental group, anti-inflammatory and anti-allergic traditional Li medicine control group, and anti-inflammatory and anti-allergic Li medicine experimental group, the ear swelling of mice after administration at the 1st, 2nd, and 3rd stimulation (10th, 13th, and 16th days) was significantly reduced.

[0100] 2. Anti-inflammatory Experiment

[0101] 60 healthy mice were divided into 6 groups: (1) 10 normal control group, distilled water was applied to the right ears; (2) 10 model control group, xylene was applied to the front and back surfaces of the right ears to induce inflammation (0.2 mL / mouse); (3) 10 positive control group, xylene was applied to the front and back surfaces of the right ears to induce inflammation (0.2 mL / mouse); (4) 10 yakapine pollen experimental group, xylene was applied to the front and back surfaces of the right ears to induce inflammation (0.2 mL / mouse); (5) 10 anti-inflammatory and anti-allergic traditional Li medicine control group, xylene was applied to the front and back surfaces of the right ears to induce inflammation (0.2 mL / mouse); (6) 10 anti-inflammatory and anti-allergic Li medicine experimental group, xylene was applied to the front and back surfaces of the right ears to induce inflammation (0.2 mL / mouse). After 1, 2, and 3 hours of inflammation, the corresponding dose of drugs were applied to the right ears of each group, and distilled water was applied to the normal control group and model group. After 4 hours, the mice were killed by dislocation of the neck and the left and right ears were cut along the base line of the auricle. The round ear pieces were removed from the same part of the left and right ears with an 8mm puncher at the same time, and then weighed on an analytical balance. The weight difference between the two ear pieces was taken as the degree of inflammation swelling, and the swelling inhibition rate was calculated. Inhibition rate % = (control group - drug group) / model control group swelling × 100%.

[0102] Table 2 Xylene-induced ear swelling experiment in mice ( , n=10)

[0103]

[0104] Note: a Compared with the normal control group, P<0.05; b Compared with the model group, P<0.05.

[0105] The effects of the Yakapine pollen experimental group, the anti-inflammatory and anti-allergic traditional Li medicine control group, and the anti-inflammatory and anti-allergic Li medicine experimental group on xylene-induced ear swelling in mice were studied. Compared with the normal control group, the ear swelling in the model control group was significantly increased, and the difference was statistically significant (P<0.05), indicating that the xylene-induced inflammation model was successfully established. The ear swelling of mice in each drug-treated group was significantly lower than that in the model control group, and the differences were statistically significant (P<0.05). Compared with the normal control group, there was no statistically significant difference. This shows that the Yakapine pollen experimental group, the anti-inflammatory and anti-allergic traditional Li medicine control group, and the anti-inflammatory and anti-allergic Li medicine experimental group can inhibit the ear swelling of mice caused by xylene and restore the ear swelling to normal. Among them, the effect of the anti-inflammatory and anti-allergic Li medicine experimental group was better than that of the Yakapine pollen and anti-inflammatory and anti-allergic traditional Li medicine control groups, but still lower than that of the positive control group.

[0106] 3. Anti-itching Experiment

[0107] Sixty healthy mice, half male and half female, weighing 18-22 g, were randomly divided into six groups: (1) normal control group (2) model control group (3) positive control group (compound dexamethasone acetate cream) (4) pine pollen experimental group (5) anti-inflammatory and anti-allergic traditional Li medicine control group (6) anti-inflammatory and anti-allergic Li medicine experimental group, 10 mice in each group. The back hair (2.5 cm × 2.5 cm) was removed with depilatory cream, and the test drugs were treated according to the following steps: (1) distilled water was applied to the normal control group; (2) distilled water was applied to the model control group; (3) compound dexamethasone cream was applied to the positive control group; (4) pine pollen experimental group was applied with pine pollen; (5) anti-inflammatory and anti-allergic traditional Li medicine control group was applied with traditional Li medicine; (6) anti-inflammatory and anti-allergic Li medicine experimental group was applied with Li medicine. Each group was treated once a day for 7 days.

[0108] Itch: 40 minutes after the last administration, 4-AP (0.02% in saline) solution was injected subcutaneously at the back of the neck of the mice at a dose of 0.01 ml / g. The mice in the normal control group were injected with saline, while the mice in the model group, positive control group, yakapine pollen experimental group, anti-inflammatory and anti-allergic traditional Li medicine control group, and anti-inflammatory and anti-allergic Li medicine experimental group were all injected with 4-AP.

[0109] Observation of itch reaction: The number of licking behaviors of mice was recorded within 10 minutes after the start of observation. Licking behavior refers to the action of mice repeatedly turning their heads and licking the backs on both sides. Continuous licking with a short pause was counted as one lick. The latency, duration and number of licking of the mice's licking reaction were observed, and the inhibition rate of the reaction was calculated. The calculation formula of the inhibition rate was: inhibition rate = (number of licking in the model control group - number of licking in the drug group) / number of licking in the model control group × 100%.

[0110] Table 3 Antipruritic test ( , n=10)

[0111]

[0112] Note: Compared with the model control group, * P<0.05, ** P<0.01.

[0113] Compared with the model control group, the number of licking times of mice in the positive control group, the pine pollen experimental group, the anti-inflammatory and anti-allergic traditional Li medicine control group, and the anti-inflammatory and anti-allergic Li medicine experimental group within 30 minutes was significantly reduced (P<0.05 or P<0.01). In addition, the number of scratching times of mice in the positive control group, the pine pollen experimental group, the anti-inflammatory and anti-allergic traditional Li medicine control group, and the anti-inflammatory and anti-allergic Li medicine experimental group within 30 minutes was also significantly reduced (P<0.01).

Claims

1. An anti-inflammatory and anti-allergic medicine, characterized in that: The specific prescription composition is as follows: 60% pine pollen, 15% lantana extract, 10% cyperus rotundus extract, 10% wormwood extract, and 5% sour vine extract, wherein the extracts are all ethanol extracts.

2. The method for preparing the Li medicine according to claim 1, characterized in that: The extracts are extracted using ethanol solution, reflux leaching under heating conditions, ethanol recovery, and freeze-drying the extracts.

3. The Li medicine according to claim 1 or the Li medicine obtained by the preparation method according to claim 2, characterized in that: The above-mentioned Li medicine can be made into one or more of tablets, capsules, powders, dispersible tablets, gels, creams, sprays, patches, plasters, injections, oral solutions, tinctures, and ointments.

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