Cell cryopreservation rack

By designing a cell freezing shelf including a sealing cover assembly, an eight-sided storage assembly and an access assembly, the problems of inconvenience and safety hazards of traditional freezing shelf in low temperature environments are solved, and an efficient and safe cell freezing process is achieved.

CN119706062AInactive Publication Date: 2025-03-28ANHUI NORMAL UNIV
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Patent Information

Application Number
CN202510217109.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-26
Publication Date
2025-03-28
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

Traditional cell freezing shelves are inconvenient to operate in low temperature environments and are prone to frostbite. The process of picking up and putting the freezing storage ducts is laborious and has great safety risks.

Method used

A cell freezing shelf including a sealing cover assembly, an eight-sided storage assembly and an access assembly were designed. The sealing cover assembly is bolted to connect the eight-side storage assembly, and the insulation assembly is placed in the middle of the eight-side storage assembly, and the access assembly is connected to the sliding inside. The design adopts mechanical structures such as cover doors, support plates and servo motors, which achieve convenient access and efficient low-temperature environment maintenance.

Benefits of technology

It improves the efficiency and safety of cell freezing, reduces the volatility of liquid nitrogen, avoids the risk of frostbite, and ensures the safety and integrity of cell samples during the access process through precise clamping and pushing mechanisms.

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Abstract

The invention discloses a cell cryopreservation rack, and belongs to the technical field of cell cryopreservation, the cell cryopreservation rack comprises a sealing cover assembly, the bottom end of the sealing cover assembly is fixedly connected with a plurality of octahedral storage assemblies, and the octahedral storage assemblies are arranged in an up-and-down connected array; a plurality of heat preservation assemblies are placed in the middle of the octahedral storage assembly, a storing and taking assembly is slidably connected into the octahedral storage assembly in a penetrating mode, and a plurality of mounting plates are fixedly connected between the sealing cover assembly and the octahedral storage assemblies through bolts. Through mutual cooperation of the octahedral storage assembly and the heat preservation assembly, efficient storage and temperature control of the cell cryopreservation tubes are achieved, the space is fully utilized, more cell cryopreservation tubes can be orderly stored in the limited space, the storage density is further improved, the octahedral storage assembly and the heat preservation assembly are perfectly combined, and the storage efficiency is improved. Not only is the storage efficiency of the cell samples improved, but also the long-term storage quality of the cell samples is effectively guaranteed.
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Description

Technical Field

[0001] The invention belongs to the technical field of cell freezing, and in particular relates to a cell freezing rack. Background Art

[0002] Cryopreservation racks are important equipment for cryopreservation of cells and related biological samples. The background technology mainly involves the principles and requirements of cell cryopreservation. Cryopreservation of cells is a technology that achieves long-term preservation of cells by placing them at extremely low temperatures to slow down their metabolic activities. In the field of modern biomedicine, cell cryopreservation technology is widely used in stem cell research, tissue engineering, regenerative medicine, organ transplantation, and assisted reproduction. In recent years, with the development of biological sample banks and the advancement of cell therapy technology, the technical requirements for cell cryopreservation racks are also constantly increasing.

[0003] During the cell freezing process, special cryopreservation racks are required to store cryotubes containing cells. Traditional cryopreservation racks are mostly drawer-type structures. Although they can hold a large number of cryotubes, there are some problems during operation. For example, operators are prone to frostbite when taking and placing cryotubes in a low-temperature environment. In addition, traditional cryopreservation racks are mostly directly hung in liquid nitrogen tanks. When they need to be taken out, the entire cryopreservation rack needs to be lifted out. This process is not only laborious, but may also cause liquid nitrogen to splash, causing safety hazards. Therefore, how to optimize the design of cryopreservation racks to improve the efficiency and safety of cell freezing has become an important direction of related technical research. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the disadvantages of the above-mentioned prior art and provide a cell freezing rack.

[0005] The technical solution adopted to solve the above technical problems is: a cell freezing rack, including a sealing cover assembly, a plurality of eight-sided storage assemblies are fixedly connected to the bottom end of the sealing cover assembly, and the plurality of eight-sided storage assemblies are arranged in an array connected up and down, a plurality of insulation assemblies are placed in the middle of the eight-sided storage assembly, and a storage and access assembly is slidably connected inside the eight-sided storage assembly, and a plurality of mounting plates are fixedly connected between the sealing cover assembly and each eight-sided storage assembly by bolts.

[0006] Furthermore, a plurality of support plates are slidably connected to the surface of the eight-sided storage component, a plurality of cover doors are rotatably connected to the surface of the eight-sided storage component, and the plurality of support plates and cover doors are located on each surface of the eight-sided storage component.

[0007] Through the above technical solution, the setting of the cover door can close the opening of the eight-sided storage assembly when not in use, reduce the volatilization of liquid nitrogen, maintain a low-temperature environment, and further improve the efficiency of cell cryopreservation. The sliding and rotating connection between the cover door and the support plate allows the operator to easily open or close the cover door and adjust the height of the support plate in a low-temperature environment, thereby facilitating the storage and access of cryopreserved tubes and avoiding the frostbite problem that may be caused by traditional cryopreservation racks when taking out and placing cryopreserved tubes.

[0008] Furthermore, the heat preservation component includes an open box body, two C-shaped plates are slidably connected through one side of the open box body, and a storage box is slidably connected to the inner side of the C-shaped plate.

[0009] Through the above technical solution, the design of the C-type plate can fit tightly against the outer wall of the storage box to form an effective insulation layer, further reducing the transfer of heat and improving the stability and efficiency of cell cryopreservation. The setting of the storage box not only provides a safe storage space for cells, but also facilitates the operator to quickly take out or put in cell samples when needed. At the same time, the sliding connection between the C-type plate and the storage box allows the operator to easily adjust the position of the storage box in a low-temperature environment, thereby facilitating the storage and access of cell samples, further improving the convenience and safety of operation.

[0010] Furthermore, the open box body has a side surface through which the C-shaped plate is provided, and the upper and lower ends thereof are rotatably connected with baffles, one end of the baffles is rotatably fitted with the surface of the storage box, and the upper and lower end surfaces of the inner side of the open box body are respectively provided with first slide grooves.

[0011] Through the above technical solution, the design of the baffle can further increase the sealing between the C-type plate and the open box body, and one end of the baffle is in rotational contact with the surface of the storage box, making it less likely to shake. At the same time, the setting of the first slide groove provides a guide for the sliding of the C-type plate, making the C-type plate smoother and less likely to get stuck when sliding, which not only improves the insulation effect of cell cryopreservation, but also further improves the convenience and stability of operation.

[0012] Furthermore, two first protrusions are fixedly connected to the outer sides of both ends of the C-shaped plate, and the two first protrusions are slidably connected to the first sliding grooves. Second protrusions are fixedly connected to the inner sides of both ends of the C-shaped plate.

[0013] Through the above technical solution, the meshing sliding connection between the two first protrusions and the first slide groove ensures the stable sliding of the C-type plate in the open box body, while providing precise guiding effect, avoiding the deviation or shaking of the C-type plate during the sliding process, making the storage and access of cell samples more efficient and convenient.

[0014] Furthermore, second slide grooves are respectively provided on the upper and lower end surfaces of the storage box, the second slide grooves are meshed and slidably connected with the second protrusions, a folding handle is rotatably connected to the middle of the side surface of the storage box, a plurality of partition cabinets are provided in the middle of the storage box, test tube fixing racks are placed inside the plurality of partition cabinets, a baffle is rotatably connected to the middle of one side of the partition cabinet close to the opening, both ends of the baffle are rotatably connected to the two side surfaces of the storage box, and buckles are provided on both sides of the partition cabinet.

[0015] Through the above technical solution, the meshing sliding connection between the second protrusion and the second slide groove further enhances the stability of the C-type plate in the storage box, ensures that it always maintains the correct position during the sliding process, and effectively prevents the risk of cell sample damage caused by shaking or offset. The design of the folding handle makes it easier for users to carry or move the storage box, and it can be folded and put away when not in use, saving space, while avoiding collision and damage to the handle during transportation. The setting of several partition cabinets not only realizes the classified storage of cell samples, but also improves the utilization rate of the internal space of the storage box, making the management of cell samples more orderly and efficient. The test tube fixing rack placed on the inside of each partition cabinet can firmly fix the test tube to prevent the test tube from breaking or leaking due to shaking during storage or transportation. The rotation connection between the two ends of the baffle and the two side surfaces of the storage box enables users to open or close the baffle more flexibly when accessing cell samples, improving the convenience of operation. The setting of the buckle further ensures the stability of the baffle when closed, preventing the baffle from accidentally opening due to external factors, thereby ensuring the safe storage of cell samples.

[0016] Furthermore, an operating panel is installed on the outer ring area of ​​the top surface of the sealing cover assembly, a sealing door is rotatably connected to the middle part of the top end of the sealing cover assembly, a threaded ring is fixedly connected to the outer ring area of ​​the bottom end of the sealing cover assembly, a connecting sleeve is fixedly connected to the bottom end of the sealing cover assembly, and the connecting sleeve is fixedly connected to the single eight-sided storage assembly through a mounting plate.

[0017] Through the above technical solution, the rotating connection design of the sealed door ensures its stability and sealing during the opening and closing process, effectively preventing external air and pollutants from entering the storage box, thereby ensuring the purity and survival rate of cell samples. The threaded ring can be tightly connected to the liquid nitrogen tank to prevent the risk of cell sample damage due to poor sealing. The connection sleeve is fixedly connected to a single eight-sided storage component through the mounting plate, which not only enhances the structural stability of the entire cell freezing rack, but also ensures the independence of each storage component, allowing users to independently manage and operate each storage component, improving the flexibility and efficiency of cell sample management.

[0018] Furthermore, the eight-sided storage assembly includes an eight-sided frame, the bottom end of which is fixedly connected to an octagonal frame, each side surface of the eight-sided frame is respectively provided with a placement opening, the top of the placement opening is rotatably connected to the cover door, and the upper and lower ends of the eight-sided frame are respectively provided with a plurality of mounting grooves, and a plurality of the eight-sided storage assemblies are fixedly connected to the bolt structure of the mounting plate through the mounting grooves, and the bottom end of the eight-sided storage assembly located at the bottom is fixedly connected to a sealing bottom plate.

[0019] Through the above technical solution, the design of the eight-sided storage component fully considers the needs and convenience of cell sample storage. The structure of the eight-sided rack enables each storage component to fully utilize the space and improve the storage density. The fixed connection of the octagonal rack enhances the stability of the entire storage component and prevents structural deformation or damage caused by external forces. The placement port is convenient for users to take and place cell samples, and the rotating connection design of the cover door ensures its stability and sealing during the opening and closing process, effectively preventing the volatilization of liquid nitrogen and the contamination of cell samples. The bolt structure of the mounting groove and the mounting plate is fixedly connected, which not only facilitates users to flexibly adjust the number and position of storage components according to actual needs, but also ensures the structural stability and safety of the entire cell freezing rack. The fixed connection of the sealed bottom plate effectively prevents the leakage of liquid nitrogen and ensures the storage environment of cell samples.

[0020] Furthermore, two square grooves are provided at the bottom ends of several of the placement openings, the square grooves are slidably connected to the support plate, a first gear is rotatably connected through the bottom end of the square groove, a transmission shaft is fixedly connected between the two first gears located on the same surface, the transmission shaft is rotatably connected to the octagonal frame, one end of the transmission shaft is rotatably connected to a servo motor, and a power output end of the servo motor is fixedly connected to the transmission shaft.

[0021] Through the above technical solution, the design of the two square grooves further improves the convenience of taking and placing cell samples. The cooperation of the first gear and the transmission shaft enables the two square grooves and the support plates thereon to move synchronously when the servo motor is started, which not only improves the degree of automation of the operation, but also ensures the stability and accuracy of the movement of the support plate. The fixed connection between the power output end of the servo motor and the transmission shaft ensures the effective transmission of power, making the entire movement process controllable and stable. Therefore, users can perform cell sample storage and access operations more efficiently and accurately, greatly improving work efficiency and the convenience of sample management.

[0022] Furthermore, the support plate includes a placement plate, the placement plate is fitted with the insulation component, the bottom end of the placement plate is fixedly connected to two sliding bars, the sliding bars are meshed and slidably connected with the square grooves, the sliding bar is provided with a tooth groove at the end away from the placement plate, the tooth groove is meshed and transmission connected with the first gear, one end of the sliding bar is fixedly connected to a limiting plate, and the limiting plate is slidably fitted with the inner surface of the octagonal frame.

[0023] Through the above technical scheme, the meshing sliding connection between the slide bar and the square groove not only enhances the stability of the support plate movement, but also enables the support plate to move smoothly along the preset path, avoiding potential damage to the cell sample due to shaking. The meshing transmission connection between the tooth groove and the first gear ensures the accuracy and synchronization of the support plate movement. When the servo motor drives the transmission shaft to rotate, the first gear drives the slide bar and the placement plate thereon to move smoothly. This precise transmission mechanism greatly improves the accuracy and reliability of the cell sample access operation. The sliding fit between the limit plate and the inner surface of the octahedral frame further limits the movement range of the support plate to prevent it from moving out of the preset position due to excessive movement, thereby ensuring the stability and safety of the entire cell freezing rack.

[0024] The cam is connected with a third gear at the middle of the telescopic end of the hydraulic push rod, and a transmission belt is meshed and connected between the second gear and the third gear. The bottom end of the third gear is fixedly connected to the medium-sized plate, the telescopic end of the hydraulic push rod is slidably connected to the medium-sized plate, and the bottom end of the telescopic end of the medium-sized plate is rotatably connected to a push block, and the two ends of the push block are rotatably connected to the first push strip, and the ends of the two ends of the medium-sized plate close to the inside are rotatably connected to the second push strip, and the two ends of the medium-sized plate are rotatably connected to the third push strip on the side away from the second push strip. The middle part of the second push strip is rotatably connected to the end of the first push strip away from the push block, and the second push strip and the third push strip are rotatably connected to a splint at one end away from the medium plate, and the splint is frictionally fitted with the surface of the insulation component.

[0025] Through the above technical solution, the cell cryopreservation tube can be accurately clamped and pushed. When in use, the operator can pull the handle to pull the entire storage and retrieval assembly out of the insulation assembly for easy operation. Start the drive motor, and its power output end drives the second gear to rotate, and the third gear rotates synchronously through the action of the transmission belt. Since the third gear is slidably connected to the telescopic end of the hydraulic push rod, the rotation of the third gear will be converted into the horizontal movement of the medium plate. During the movement of the medium plate, through the cooperation of the push block and the first push bar, and the linkage of the second push bar and the third push bar, the clamping plate can approach the inner wall of the insulation assembly, thereby clamping the cell cryopreservation tube placed in the insulation assembly. The telescopic function of the hydraulic push rod further adjusts the clamping force between the clamping plate and the cell cryopreservation tube, ensuring that the cell cryopreservation tube will neither slip nor be damaged during the storage and retrieval process. This design not only improves the storage and retrieval efficiency of the cell cryopreservation tube, but also ensures the safety and integrity of the cell sample.

[0026] The beneficial effects of the present invention are as follows: 1. The present invention realizes the rapid and safe storage and retrieval of cell cryopreservation tubes through an innovative mechanical structure design. The operator can easily pull out the storage and retrieval component from the insulation component by the convenient operation of pulling the handle. This design greatly simplifies the storage and retrieval process and improves work efficiency.

[0027] 2. The present invention achieves precise clamping of cell cryopreservation tubes by utilizing the synergistic effect of the drive motor, gear transmission and hydraulic push rod. This clamping method is not only stable and reliable, but also can be flexibly adjusted according to different sizes and shapes of cell cryopreservation tubes, ensuring the absolute safety of cell samples during storage and access. In addition, the telescopic function of the hydraulic push rod provides a fine adjustment space for the clamping force, effectively avoiding the risk of cell cryopreservation tubes being damaged due to over-tight clamping or slipping due to over-loose clamping.

[0028] 3. The present invention realizes efficient storage and temperature control of cell cryopreservation tubes through the cooperation of the eight-sided storage component and the heat preservation component. The design of the eight-sided storage component makes full use of the space, so that more cell cryopreservation tubes can be stored in a limited space in an orderly manner, further improving the storage density. The heat preservation component adopts efficient heat preservation materials to ensure that the cell cryopreservation tubes can maintain a stable low-temperature environment during storage, which is crucial for maintaining the activity and integrity of cell samples. The perfect combination of the two not only improves the storage efficiency of cell samples, but also effectively guarantees the long-term preservation quality of cell samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 is a first structural schematic diagram of the present invention; Figure 2 is a second structural schematic diagram of the present invention; Figure 3 is a first structural schematic diagram of the thermal insulation assembly of the present invention; Figure 4 is a second structural schematic diagram of the thermal insulation assembly of the present invention; Figure 5 It is a schematic diagram of the open box structure of the present invention; Figure 6 It is a schematic diagram of the C-type plate structure of the present invention; Figure 7 It is a schematic diagram of the storage box structure of the present invention; Figure 8 It is a schematic diagram of the first structure of the octahedral frame of the present invention; Fig. 9 It is a second structural schematic diagram of the octahedral frame of the present invention; Fig.10 It is a schematic diagram of the support plate structure of the present invention; Fig.11 is a schematic diagram of the access component structure of the present invention from a first perspective; Fig.12 It is a schematic diagram of the access component structure from a second viewing angle of the present invention.

[0030] Figure numerals: 1, sealing cover assembly; 101, operation panel; 102, sealing door; 103, threaded ring; 104, connecting sleeve; 2, eight-sided storage assembly; 201, eight-sided rack; 202, placement port; 203, octagonal rack; 204, installation slot; 205, square slot; 206, first gear; 207, transmission shaft; 208, servo motor; 3, insulation assembly; 301, opening box; 302, stop bar; 303, first slide slot; 4, support plate; 401, placement plate; 402, slide bar; 403, limit plate; 404, tooth slot; 5, cover door; 6, access assembly; 601, octagonal plate; 602, handle; 603, drive motor; 604, hydraulic push rod; 605, transmission belt; 606, second gear; 607, third gear; 608, medium-sized plate; 609, clamping plate; 610, push block; 611, first push strip; 612, second push strip; 613, third push strip; 7, mounting plate; 8, C-type plate; 801, first protrusion; 802, second protrusion; 9, storage box; 901, folding handle; 902, baffle; 903, buckle; 904, second slide; 905, partition cabinet; 10, test tube fixing rack; 11, sealing bottom plate. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0032] like Figures 1 to 12 As shown, a cell freezing rack of the present embodiment includes a sealing cover assembly 1, a plurality of eight-sided storage assemblies 2 are fixedly connected to the bottom end of the sealing cover assembly 1, the plurality of eight-sided storage assemblies 2 are arranged in an array connected up and down, a plurality of insulation assemblies 3 are placed in the middle of the eight-sided storage assembly 2, a storage and access assembly 6 is slidably connected through the inside of the eight-sided storage assembly 2, a plurality of mounting plates 7 are fixedly connected between the sealing cover assembly 1 and each eight-sided storage assembly 2 by bolts, and the required eight-sided rack 201 is installed according to the size of the liquid nitrogen tank and the required storage quantity, thereby further improving the storage density.

[0033] like Figure 1 to Figure 2As shown, a plurality of support plates 4 are slidably connected to the surface of the eight-sided storage component 2, and a plurality of cover doors 5 are rotatably connected to the surface of the eight-sided storage component 2. The plurality of support plates 4 and the cover doors 5 are located on each surface of the eight-sided storage component 2. The setting of the cover doors 5 can close the opening of the eight-sided storage component 2 when not in use, thereby reducing the volatilization of liquid nitrogen, maintaining a low-temperature environment, and further improving the efficiency of cell freezing.

[0034] like Figure 2 As shown, an operation panel 101 is installed on the outer ring area of ​​the top surface of the sealing cover assembly 1, a sealing door 102 is rotatably connected to the middle part of the top end of the sealing cover assembly 1, a threaded ring 103 is fixedly connected to the outer ring area of ​​the bottom end of the sealing cover assembly 1, and a connecting sleeve 104 is fixedly connected to the bottom end of the sealing cover assembly 1. The connecting sleeve 104 is fixedly connected to the single eight-sided storage assembly 2 through the mounting plate 7. The connecting sleeve 104 is fixedly connected to the single eight-sided storage assembly 2 through the mounting plate 7, which not only enhances the structural stability of the entire cell freezing rack, but also ensures the independence of each storage assembly, so that the user can manage and operate each storage assembly independently, thereby improving the flexibility and efficiency of cell sample management.

[0035] like Figure 3 As shown, the insulation component 3 includes an open box body 301, and two C-shaped plates 8 are slidably connected to one side of the open box body 301. A storage box 9 is slidably connected to the inner side of the C-shaped plate 8. The design of the C-shaped plate 8 can fit tightly against the outer wall of the storage box 9 to form an effective insulation layer, further reducing the transfer of heat and improving the stability and efficiency of cell freezing.

[0036] like Figures 3 to 6 As shown, two first protrusions 801 are fixedly connected to the outer sides of both ends of the C-shaped plate 8, and the two first protrusions 801 are meshed and slidably connected with the first slide groove 303. Second protrusions 802 are fixedly connected to the inner sides of both ends of the C-shaped plate 8. The meshing and sliding connection of the two first protrusions 801 with the first slide groove 303 ensures the stable sliding of the C-shaped plate 8 in the open box 301, and at the same time provides a precise guiding effect, avoiding the deviation or shaking of the C-shaped plate 8 during the sliding process, making the storage and access of cell samples more efficient and convenient.

[0037] like Figures 3 to 7 As shown, the open box body 301 passes through one side surface of which the C-shaped plate 8 is provided, and the upper and lower ends thereof are rotatably connected with baffle bars 302, and one end of the baffle bar 302 is rotatably fitted with the surface of the storage box 9, and the upper and lower end surfaces of the inner side of the open box body 301 are respectively provided with first slide grooves 303, and the setting of the first slide grooves 303 provides guidance for the sliding of the C-shaped plate 8, so that the C-shaped plate 8 slides more smoothly and is not prone to jamming, which not only improves the insulation effect of cell cryopreservation, but also further improves the convenience and stability of operation.

[0038] like Figures 3 to 7 As shown, second slide grooves 904 are respectively provided on the upper and lower end surfaces of the storage box 9, and the second slide grooves 904 are meshed and slidably connected with the second protrusion 802. A folding handle 901 is rotatably connected to the middle of the side surface of the storage box 9, and a plurality of partition cabinets 905 are provided in the middle of the storage box 9. Test tube fixing racks 10 are placed inside the plurality of partition cabinets 905. A baffle 902 is rotatably connected to the middle of one side of the partition cabinet 905 close to the opening, and both ends of the baffle 902 are rotatably connected to the two side surfaces of the storage box 9. Buckles 903 are provided on both sides of the partition cabinet 905. The setting of the buckle 903 further ensures the stability of the baffle 902 when it is closed, prevents the baffle 902 from being accidentally opened due to external factors, and thereby ensures the safe storage of cell samples.

[0039] like Figures 8 to 9 As shown, the eight-sided storage assembly 2 includes an eight-sided frame 201, the bottom end of the eight-sided frame 201 is fixedly connected to an octagonal frame 203, each side surface of the eight-sided frame 201 is respectively provided with a placement port 202, the top of the placement port 202 is rotatably connected to the cover door 5, and the upper and lower ends of the eight-sided frame 201 are respectively provided with a plurality of mounting grooves 204, and a plurality of eight-sided storage assemblies 2 are fixedly connected to the bolt structure of the mounting plate 7 through the mounting grooves 204, and the bottom end of the eight-sided storage assembly 2 located at the bottom is fixedly connected to the sealing bottom plate 11, which effectively prevents the volatilization of liquid nitrogen and the contamination of cell samples. The mounting groove 204 is fixedly connected to the bolt structure of the mounting plate 7, which is not only convenient for users to flexibly adjust the number and position of storage assemblies according to actual needs, but also ensures the structural stability and safety of the entire cell freezing rack, and the fixed connection of the sealing bottom plate 11 effectively prevents the leakage of liquid nitrogen and ensures the storage environment of cell samples.

[0040] like Figures 8 to 9 As shown, two square grooves 205 are provided at the bottom of the plurality of placement openings 202, and the square grooves 205 are slidably connected to the support plate 4, and a first gear 206 is rotatably connected to the bottom of the square grooves 205, and a transmission shaft 207 is fixedly connected between the two first gears 206 located on the same surface, and the transmission shaft 207 is rotatably connected to the octagonal frame 201, and a servo motor 208 is rotatably connected to one end of the transmission shaft 207, and a power output end of the servo motor 208 is fixedly connected to the transmission shaft 207. The design of the two square grooves 205 further improves the convenience of taking and placing cell samples, and the cooperation of the first gear 206 and the transmission shaft 207 enables the two square grooves 205 and the support plate 4 thereon to move synchronously when the servo motor 208 is started, which not only improves the degree of automation of the operation, but also ensures the stability and accuracy of the movement of the support plate 4.

[0041] like Fig.10As shown, the support plate 4 includes a placement plate 401, which is fitted with the insulation component 3. The bottom end of the placement plate 401 is fixedly connected to two slide bars 402, which are meshed and slidably connected with the square groove 205. The slide bar 402 is provided with a tooth groove 404 at the end away from the placement plate 401, and the tooth groove 404 is meshed and transmission-connected with the first gear 206. One end of the slide bar 402 is fixedly connected to a limit plate 403, and the limit plate 403 is slidably fitted with the inner surface of the octahedral frame 201. When the servo motor 208 drives the transmission shaft 207 to rotate, the first gear 206 will drive the slide bar 402 and the support plate 4 thereon to move smoothly. This precise transmission mechanism greatly improves the accuracy and reliability of the cell sample access operation.

[0042] like Figure 11 to Figure 12 As shown, the storage and retrieval assembly 6 includes an octagonal plate 601, a handle 602 is fixedly connected to the middle of the top of the octagonal plate 601, a driving motor 603 and a hydraulic push rod 604 are fixedly connected to the middle of the octagonal plate 601, a second gear 606 is fixedly connected to the power output end of the driving motor 603, a third gear 607 is slidably connected to the middle of the telescopic end of the hydraulic push rod 604, a transmission belt 605 is meshed and driven between the second gear 606 and the third gear 607, a medium-sized plate 608 is fixedly connected to the bottom of the third gear 607, and the telescopic end of the hydraulic push rod 604 is connected to the medium-sized plate 608. Through the sliding connection, the bottom end of the telescopic end of the medium-sized plate 608 is rotatably connected to a push block 610, and both ends of the push block 610 are rotatably connected to the first push strip 611, and the two ends of the medium-sized plate 608 close to the inside are rotatably connected to the second push strip 612, and the two ends of the medium-sized plate 608 are rotatably connected to the side away from the second push strip 612. The third push strip 613 is rotatably connected, and the middle part of the second push strip 612 is rotatably connected to the end of the first push strip 611 away from the push block 610, and the second push strip 612 and the third push strip 613 are rotatably connected to the end away from the medium-sized plate 608 with a splint 609, and the splint 609 is frictionally fitted with the surface of the insulation component 3.

[0043] The working principle of this embodiment is as follows: The staff installs the eight-sided storage assembly 2 at the bottom of the sealing cover assembly 1 through several installation plates 7, and installs the required eight-sided rack 201 according to the size of the liquid nitrogen tank and the required storage quantity. Finally, the sealing bottom plate 11 is installed at the bottom of the eight-sided rack 201 at the bottom to achieve the sealing of the device. Subsequently, the threaded ring 103 at the bottom of the sealing cover assembly 1 and the threaded structure at the top of the liquid nitrogen tank are installed and fixed to achieve the liquid nitrogen storage effect. The staff inserts each cell storage test tube into the test tube holder 10, and then places the test tube holder 10 on each partition cabinet 905, and then rotates the baffle 902 to fit with the surface of the storage box 9. At this time, the buckle 903 presses the baffle 902 and fixes it to prevent the test tube from falling off. Since the second protrusion 802 on the inner side of the C-type plate 8 engages and slides with the second slide groove 904 at both ends of the storage box 9, the storage box 9 is rotated to rotate it into the C-type plate 8, and the first protrusion 801 on the outer side of the C-type plate 8 engages and slides with the first slide groove 303 inside the insulation component 3, so that the C-type plate 8 and the storage box 9 enter the open box body 301, and the baffle 302 is moved to perform a limiting operation to prevent the C-type plate 8 and the storage box 9 from falling out, thereby ensuring the safety of the cells.

[0044] When it is necessary to access the cell cryopreservation tube, first lift the octagonal plate 601 through the handle 602, start the drive motor 603, and the power output end of the drive motor 603 drives the second gear 606 to rotate. The second gear 606 drives the third gear 607 to rotate through the meshing transmission of the transmission belt 605. When the third gear 607 rotates, it drives the medium-sized plate 608 to move up and down along the telescopic end of the hydraulic push rod 604. At the same time, the medium-sized plate 608 is connected to the first push bar 611 through the second push bar 612 at both ends, and the first push bar 611 is connected to the push block 610 through rotation, so that the push block 610 can rotate along the telescopic end of the medium-sized plate 608, thereby pushing the first push bar 611 to expand to both sides. When the first push bar 611 is expanded, it drives the second push bar 612 to rotate. One end of the strip 612 moves away from the medium plate 608, and the other end of the second push strip 612 drives the clamp 609 to move away from the insulation component 3, so that the clamp 609 is separated from the surface of the insulation component 3. At this time, the cell cryopreservation tube can be placed in the insulation component 3, and the drive motor 603 is started again to reverse, so that the clamp 609 moves toward the insulation component 3 and frictionally fits, so as to clamp and fix the cell cryopreservation tube. At the same time, the hydraulic push rod 604 is started, and the telescopic end of the hydraulic push rod 604 pushes the medium plate 608 to move downward. The medium plate 608 is connected to the clamp 609 through the rotation of the third push strip 613, which pushes the clamp 609 to further clamp and fix the cell cryopreservation tube, thereby ensuring the stability of the cell cryopreservation tube during the storage and retrieval process.

[0045] The storage and retrieval component 6 that clamps the insulation component 3 is placed into the eight-sided storage component 2 along the opened sealing door 102. At this time, the support plate 4 without the insulation component 3 is smoothly slid along the square groove 205 to an empty position in the eight-sided frame 201 of the device through the first gear 206 driven by the servo motor 208 at the bottom. During this process, the cover door 5 on the top of the placement port 202 falls down to close the opened placement port 202 to prevent liquid nitrogen and cold air from entering. At this time, the bottom of the insulation component 3 contacts the placement plate 401 of the support plate 4. At this time, the storage and retrieval component 6 releases the insulation component 3, and the insulation component 3 falls smoothly onto the support plate 4. Then the servo motor 208 reverses, and the support plate 4 drives the insulation component 3 to move toward the outside of the eight-sided frame 201, and the insulation component 3 pushes the cover door 5 open, and the insulation component 3 contacts the cold air formed by the liquid nitrogen for frozen storage.

[0046] The above description is only a preferred embodiment of the present invention and is not intended to limit the protection scope of the present invention.

Claims

1. A cell freezing rack, comprising a sealing cover assembly (1), characterized in that: The bottom end of the sealing cover assembly (1) is fixedly connected to a plurality of eight-sided storage assemblies (2), the plurality of eight-sided storage assemblies (2) are arranged in an array connected up and down, a plurality of heat preservation assemblies (3) are placed in the middle of the eight-sided storage assembly (2), a storage and access assembly (6) is slidably connected to the inside of the eight-sided storage assembly (2), and a plurality of mounting plates (7) are fixedly connected between the sealing cover assembly (1) and each of the eight-sided storage assemblies (2) by bolts; A plurality of support plates (4) are slidably connected to the surface of the eight-sided storage component (2), and a plurality of cover doors (5) are rotatably connected to the surface of the eight-sided storage component (2), and the plurality of support plates (4) and cover doors (5) are located on each surface of the eight-sided storage component (2); The heat preservation assembly (3) comprises an open box body (301), two C-shaped plates (8) are slidably connected to one side of the open box body (301), and a storage box (9) is slidably connected to the inner side of the C-shaped plate (8); The open box (301) has a surface on one side through which the C-shaped plate (8) is provided, and the upper and lower ends thereof are rotatably connected to stop bars (302), one end of the stop bar (302) is rotatably fitted to the surface of the storage box (9), and the upper and lower end surfaces of the inner side of the open box (301) are respectively provided with first slide grooves (303).

2. A cell freezing rack according to claim 1, characterized in that: Two first protrusions (801) are fixedly connected to the outer sides of both ends of the C-shaped plate (8), and the two first protrusions (801) are both engaged and slidably connected with the first sliding groove (303). Second protrusions (802) are fixedly connected to the inner sides of both ends of the C-shaped plate (8).

3. A cell freezing rack according to claim 1, characterized in that: The upper and lower end surfaces of the storage box (9) are respectively provided with second slide grooves (904), the second slide grooves (904) are meshed and slidably connected with the second protrusions (802), the storage box (9) is rotatably connected with a folding handle (901) penetrating the middle of the side surface, a plurality of partition cabinets (905) are provided in the middle of the storage box (9), a test tube fixing rack (10) is placed inside the plurality of partition cabinets (905), a baffle (902) is rotatably connected to the middle of one side of the partition cabinet (905) close to the opening, the two ends of the baffle (902) are rotatably connected to the two side surfaces of the storage box (9), and buckles (903) are provided on both sides of the partition cabinet (905).

4. A cell freezing rack according to claim 1, characterized in that: An operating panel (101) is installed on the outer ring area of ​​the top surface of the sealing cover assembly (1), a sealing door (102) is rotatably connected to the middle part of the top of the sealing cover assembly (1), a threaded ring (103) is fixedly connected to the outer ring area of ​​the bottom end of the sealing cover assembly (1), and a connecting sleeve (104) is fixedly connected to the bottom end of the sealing cover assembly (1), and the connecting sleeve (104) is fixedly connected to the single eight-sided storage assembly (2) via a mounting plate (7).

5. A cell freezing rack according to claim 1, characterized in that: The eight-sided storage assembly (2) comprises an eight-sided frame (201), the bottom end of the eight-sided frame (201) is fixedly connected to an octagonal frame (203), each side surface of the eight-sided frame (201) is respectively provided with a placement opening (202), the top end of the placement opening (202) is rotatably connected to a cover door (5), and the upper and lower ends of the eight-sided frame (201) are respectively provided with a plurality of mounting grooves (204), and a plurality of the eight-sided storage assemblies (2) are fixedly connected to a bolt structure of a mounting plate (7) via the mounting grooves (204), and the bottom end of the eight-sided storage assembly (2) at the bottommost end is fixedly connected to a sealing bottom plate (11).

6. A cell freezing rack according to claim 5, characterized in that: Two square grooves (205) are provided at the bottom ends of a plurality of the placement openings (202), the square grooves (205) are slidably connected to the support plate (4), a first gear (206) is rotatably connected to the bottom end of the square groove (205), a transmission shaft (207) is fixedly connected between two first gears (206) located on the same surface, the transmission shaft (207) is rotatably connected to the octagonal frame (201), one end of the transmission shaft (207) is rotatably connected to a servo motor (208), and a power output end of the servo motor (208) is fixedly connected to the transmission shaft (207).

7. A cell freezing rack according to claim 1, characterized in that: The support plate (4) comprises a placement plate (401), the placement plate (401) is fitted with the heat preservation component (3), the bottom end of the placement plate (401) is fixedly connected to two slide bars (402), the slide bars (402) are meshed and slidably connected with the square grooves (205), a tooth groove (404) is provided at one end of the slide bar (402) away from the placement plate (401), the tooth groove (404) is meshed and transmission-connected with the first gear (206), one end of the slide bar (402) is fixedly connected to a limit plate (403), and the limit plate (403) is slidably fitted with the inner surface of the octagonal frame (201).

8. A cell freezing rack according to claim 1, characterized in that: The storage and retrieval assembly (6) comprises an octagonal plate (601), a handle (602) is fixedly connected to the middle of the top of the octagonal plate (601), a driving motor (603) and a hydraulic push rod (604) are fixedly connected through the middle of the octagonal plate (601), a second gear (606) is fixedly connected to the power output end of the driving motor (603), a third gear (607) is slidably connected through the middle of the telescopic end of the hydraulic push rod (604), a transmission belt (605) is meshed and transmission-connected between the second gear (606) and the third gear (607), a middle plate (608) is fixedly connected to the bottom of the third gear (607), and the telescopic end of the hydraulic push rod (604) and the middle plate (608) are penetrated. The middle part of the second push strip (612) is rotatably connected to the end of the first push strip (611) away from the push strip (610); the second push strip (612) and the third push strip (613) are rotatably connected to the surface of the heat preservation component (3); the middle part of the second push strip (612) is rotatably connected to the end of the first push strip (611) away from the push strip (610); the second push strip (612) and the third push strip (613) are rotatably connected to the end of the medium plate (608) away from the push strip (610); the second push strip (612) and the third push strip (613) are rotatably connected to the splint (609); the splint (609) is frictionally fitted with the surface of the heat preservation component (3).

Citation Information

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